Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0000540

Physical Location & Seq

Genomic Coordinates Reverse (-)

41028996 .. 41035116

Gene Structure 6121 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1333100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1773 bp

ATGGAAAAATGGCTTGGAAAAATGAGATGCTTAGTTGTCGACGCAGATAAGAAACTGAAACTGTCGTCTGATCAGGAAACGGTCAAGAAATATTTGAGTGAGCTAAGGGAAGTCATTTATGAAGCCGACAACGCATTGACGGATTGCCTGATCAGTGAGGAAGAAAAGAAGAAAAAGAGGTTCTTCTCATGCTTGTTGCCTCTGGATCCGGTTTTCCCGCATGCGATGGGCAGGAGGTTGACGAAGATTAATTCTGATATGAAGAACGTGCACGACGTGTTGATCAACTATTTTAGTGCATCAGCTGCTGCACCAGATAGTAGTATCAGCCAACAAATCAGCAGCAGCTGGCGAATTGTTGGTTCACTAAACCCACATAAGACATATGGATTGGATGAGGACATAGAAAAGTTGAAAGGGTGGATACTTAATGACGACGAGAAGGAACCAGCAATGAACTACATTGGCATTGTGGGGATGGGGGGCTTGGGCAAAACCACCCTTGCTCAACAACTCTTTAACGATTCTCAAATCTTGGATCACTTTGAAAAGACAATGTGGGTCTGTGTTTCAGGAAATTTCTGCGAGCAGTGGATCCTGAAGAAGTTACTCAACAAAGTTAATGAAGAAACGTCTTCCTTGGATAGTGATGAAATTTTGCGCAGACTCATCACTGTCCTTAACGGTAAATCCTATCTCATTGTCCTGGATGATGTGTGGCCTTACAAAAACAAATCAGATTGGTTGAAAGACCTGTGCTCCCAGCTACCAACATCAGTTGGTAAATCCAGCTGCATCATTATTACCACCAGGATTGAAGACGTTGCCCAAAAAATGGTTCTTAGAAACACACAAATTCATCACCATAAACCTCTAGAGAACGAAGACAGCTGGGATTTATTCAGTGAATATGCATTTCGAAAAAGCAATGGAAAATGCCCTAGTGAGCATTATAAAGATGTCTCAGTGCATATCTTGAAGAAATGTGGTGGCCTTCCTCTGGCAATAAAGACTTTAGGAAGCTTATTGTCTGAGAAGGTTGGTTCTCCTTCAGAATGGACAGACATTTCGGACAAATTCCATGAACTCACAAAAAAAGGAAAAACTGACTCTGTCATCGACTCTCTGCAGTTAAGCTATGATGAACTTCCGCATACCAGTCTCAAGCAATGCCTATTGTGTCTCTCCATTTATCCTGAAGACTTTGAGATAGATGCGGAACAGTTAATTCACTGGTGGATTGGGGAGGGCATCGTCCGGGAAAAGGGCTCAACAACAGCTATAGAATTGGGATTTGAATATCTCGCAGAATTAGTGAACAGAAGCCTGCTGGAAGTAGTGGATCGGCGATGGTACGATGGGAAAGTTTATAAGTGCAAGATACACGATATGGTGCGGGAGGTAATTATCAAGATTGCGGGAGAGGAGGCATTTTGCATCTTCAATGAGCAAGGAAAGCTAATGCAGAAACATGAGCAAAATGAGCAAAAGCCACGATGGTTCCGTATCAGCGAAGACATGGATGATGACGAGAAGGAACTAGAAGATAATCCAAAGTTGAGGACATTTTTTCTCATGTCAAGCCCTCCTAATAACTTTGGCAAGAATTTTAGGTTCCTGGAGTCCCTCAGGGTGTTGGACTTGTCGTACTCTAAAGTTGTCGAAGATAGCAGCAGCCCGTCTCCAGGTAGAACGCACTTTAAATTCTTCATCATGCTTTCTAATTTCGATCTATTGAGTGTGTACACTTGTGTATGTGATGTTTGTGCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0008150 GO:0009605 GO:0009607 GO:0009617 GO:0009987 GO:0016020 GO:0023052 GO:0042742 GO:0043207 GO:0043621 GO:0044464 GO:0050789 GO:0050794 GO:0050829 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0065007 GO:0071944 GO:0098542

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

NB-ARC

Protein Analysis

591

Amino Acids

67.76

Weight (kDa)

5.59

Isoelectric Point (pI)

45.47

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Rx_N	PF18052	5 - 61	5.7e-06	Rx N-terminal domain
NB-ARC	PF00931	133 - 309	4.8e-39	NB-ARC domain
WHD_DRP	PF23559	397 - 469	2.2e-18	Disease resistance protein Winged helix domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000540)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G50950 AT3G50950
fragaria_vesca	FvH4_1g12710 FvH4_1g12710 FvH4_3g00820 FvH4_3g00820 FvH4_3g00820 FvH4_3g00820
malus_domestica	MD02G1141100.v1.1 MD10G1333100.v1.1
prunus_persica	Prupe.4G009300_v2.0.a1 Prupe.4G009300_v2.0.a1 Prupe.4G009300_v2.0.a1 Prupe.6G150700_v2.0.a1 Prupe.7G160100_v2.0.a1 Prupe.7G160100_v2.0.a1
pyrus_communis	pycom02g11110 pycom10g28240 pycom10g28340 pycom10g28360 pycom10g28450
rosa_chinensis	RchiOBHm_Chr2g0100711 RchiOBHm_Chr3g0485861 RchiOBHm_Chr4g0391451 RchiOBHm_Chr4g0391591 RchiOBHm_Chr4g0391601 RchiOBHm_Chr4g0391681 RchiOBHm_Chr5g0001141 RchiOBHm_Chr5g0001221
rosa_laevigata	RLG00000017001
rosa_multiflora	Rmu_co8260007.1_g000001 Rmu_co8260103.1_g000001 Rmu_co8519155.1_g000001 Rmu_sc0000060.1_g000050 Rmu_sc0000255.1_g000036 Rmu_sc0001174.1_g000023 Rmu_sc0001174.1_g000025 Rmu_sc0001659.1_g000009 Rmu_sc0001706.1_g000009 Rmu_sc0001706.1_g000024 Rmu_sc0001706.1_g000035 Rmu_sc0004189.1_g000018 Rmu_sc0004189.1_g000030 Rmu_sc0004862.1_g000002 Rmu_sc0004876.1_g000038 Rmu_sc0023741.1_g000004
rosa_roxburghii	Rroxscaffold_1G00075100 Rroxscaffold_1G00075160 Rroxscaffold_1G00075210 Rroxscaffold_5G00337160 Rroxscaffold_5G00386590 Rroxscaffold_6G00396300
rosa_rugosa	Rorug03G0336200 Rorug03G0336300 Rorug04G0389400
rosa_samantha	Rh2AG140400 Rh2BG144100 Rh2CG145400 Rh2DG144900 Rh3AG271200 Rh4AG038000 Rh4AG038700 Rh4BG032000 Rh4BG032800 Rh4CG041100 Rh4CG041300 Rh4DG035500 Rh4DG035600 Rh5AG008800 Rh5AG009100 Rh5BG010900 Rh5BG011500 Rh5BG011600 Rh5BG012000 Rh5CG009400 Rh5CG010000 Rh5DG009800
rosa_wichuraiana	Rw2G010940 Rw4G002950 Rw4G002990 Rw4G003000 Rw5G000880 Rw5G000950 Rw5G000960

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	954, 1371
Acc16I	TGCGCA	1 cut(s)	662
AccB7I	CCANNNNNTGG	1 cut(s)	835
AccI	GTMKAC	1 cut(s)	39
AciI	CCGC	5 cut(s)	218, 1151, 1217, 1396, 1418
AclWI	GGATC	6 cut(s)	200, 213, 546, 589, 602, 1350
AcsI	RAATTY	6 cut(s)	577, 654, 855, 1076, 1606, 1703
AcuI	CTGAAG	3 cut(s)	620, 1035, 1218
AdeI	CACNNNGTG	1 cut(s)	277
AfaI	GTAC	3 cut(s)	1355, 1649, 1745
AfiI	CCNNNNNNNGG	4 cut(s)	835, 1000, 1264, 1685
AflIII	ACRYGT	1 cut(s)	276
AgsI	TTSAA	7 cut(s)	415, 548, 748, 818, 979, 1298, 1444
AjiI	CACGTC	1 cut(s)	277
AjnI	CCWGG	4 cut(s)	705, 809, 1617, 1684
AjuI	GAANNNNNNNTTGG	3 cut(s)	29, 822, 854
Alw21I	GWGCWC	2 cut(s)	273, 761
Alw26I	GTCTC	4 cut(s)	967, 1166, 1187, 1686
Alw44I	GTGCAC	1 cut(s)	269
AlwI	GGATC	6 cut(s)	200, 213, 546, 589, 602, 1350
AlwNI	CAGNNNCTG	2 cut(s)	308, 348
AoxI	GGCC	2 cut(s)	719, 991
ApaLI	GTGCAC	1 cut(s)	269
ApeKI	GCWGC	7 cut(s)	305, 308, 342, 345, 792, 1671, 1674
ApoI	RAATTY	6 cut(s)	577, 654, 855, 1076, 1606, 1703
ArsI	GACNNNNNNTTYG	2 cut(s)	1051, 1083
AseI	ATTAAT	1 cut(s)	249
AspLEI	GCGC	1 cut(s)	663
AsuC2I	CCSGG	1 cut(s)	1259
AsuHPI	GGTGA	1 cut(s)	854
AsuII	TTCGAA	1 cut(s)	919
AxyI	CCTNAGG	1 cut(s)	1628
BaeGI	GKGCMC	1 cut(s)	273
BamHI	GGATCC	2 cut(s)	205, 594
BanII	GRGCYC	1 cut(s)	1271
BbsI	GAAGAC	5 cut(s)	627, 825, 891, 1206, 1521
Bbv12I	GWGCWC	2 cut(s)	273, 761
BbvI	GCAGC	7 cut(s)	292, 295, 354, 357, 779, 1683, 1686
BccI	CCATC	5 cut(s)	220, 472, 1344, 1352, 1491
BciT130I	CCWGG	4 cut(s)	707, 811, 1619, 1686
BciVI	GTATCC	1 cut(s)	417
BclI	TGATCA	3 cut(s)	70, 150, 282
BcnI	CCSGG	1 cut(s)	1259
BcoDI	GTCTC	4 cut(s)	967, 1166, 1187, 1686
BfaI	CTAG	3 cut(s)	875, 942, 1541
BfmI	CTRYAG	2 cut(s)	1127, 1281
BfuI	GTATCC	1 cut(s)	417
BisI	GCNGC	7 cut(s)	306, 309, 343, 346, 793, 1672, 1675
BlsI	GCNGC	7 cut(s)	307, 310, 344, 347, 794, 1673, 1676
Bme1390I	CCNGG	5 cut(s)	707, 811, 1259, 1619, 1686
BmgBI	CACGTC	1 cut(s)	277
BmiI	GGNNCC	5 cut(s)	207, 447, 596, 1502, 1616
BmrFI	CCNGG	5 cut(s)	707, 811, 1259, 1619, 1686
BmsI	GCATC	6 cut(s)	17, 308, 804, 1204, 1260, 1446
BpiI	GAAGAC	5 cut(s)	627, 825, 891, 1206, 1521
BpmI	CTGGAG	2 cut(s)	1640, 1668
Bpu10I	CCTNAGC	1 cut(s)	104
Bpu14I	TTCGAA	1 cut(s)	919
BpuEI	CTTGAG	1 cut(s)	1148
BpuMI	CCSGG	1 cut(s)	1259
BsaJI	CCNNGG	1 cut(s)	639
BsaWI	WCCGGW	1 cut(s)	208
BsaXI	ACNNNNNCTCC	2 cut(s)	743, 773
Bsc4I	CCNNNNNNNGG	4 cut(s)	835, 1000, 1264, 1685
Bse1I	ACTGG	2 cut(s)	1158, 1238
Bse21I	CCTNAGG	1 cut(s)	1628
Bse3DI	GCAATG	3 cut(s)	459, 934, 1175
BseBI	CCWGG	4 cut(s)	707, 811, 1619, 1686
BseDI	CCNNGG	1 cut(s)	639
BseGI	GGATG	4 cut(s)	400, 483, 715, 1528
BseLI	CCNNNNNNNGG	4 cut(s)	835, 1000, 1264, 1685
BseMI	GCAATG	3 cut(s)	459, 934, 1175
BseMII	CTCAG	3 cut(s)	978, 1023, 1642
BseNI	ACTGG	2 cut(s)	1158, 1238
BseRI	GAGGAG	1 cut(s)	1439
BseSI	GKGCMC	1 cut(s)	273
BseXI	GCAGC	7 cut(s)	292, 295, 354, 357, 779, 1683, 1686
BseYI	CCCAGC	2 cut(s)	762, 891
BsgI	GTGCAG	1 cut(s)	294
BshFI	GGCC	2 cut(s)	721, 993
BsiHKAI	GWGCWC	2 cut(s)	273, 761
BsiSI	CCGG	2 cut(s)	209, 1258
BslFI	GGGAC	1 cut(s)	1609
BslI	CCNNNNNNNGG	4 cut(s)	835, 1000, 1264, 1685
BsmAI	GTCTC	4 cut(s)	967, 1166, 1187, 1686
BsmBI	CGTCTC	1 cut(s)	1686
BsmFI	GGGAC	1 cut(s)	1609
BsnI	GGCC	2 cut(s)	721, 993
Bsp119I	TTCGAA	1 cut(s)	919
Bsp1286I	GDGCHC	3 cut(s)	273, 761, 1271
Bsp1407I	TGTACA	1 cut(s)	1743
Bsp143I	GATC	8 cut(s)	70, 150, 205, 282, 538, 594, 1342, 1729
BspACI	CCGC	5 cut(s)	218, 1151, 1217, 1396, 1418
BspANI	GGCC	2 cut(s)	721, 993
BspCNI	CTCAG	3 cut(s)	977, 1024, 1641
BspLI	GGNNCC	5 cut(s)	207, 447, 596, 1502, 1616
BspMAI	CTGCAG	1 cut(s)	1131
BspPI	GGATC	6 cut(s)	200, 213, 546, 589, 602, 1350
BspT104I	TTCGAA	1 cut(s)	919
BsrDI	GCAATG	3 cut(s)	459, 934, 1175
BsrGI	TGTACA	1 cut(s)	1743
BsrI	ACTGG	2 cut(s)	1158, 1238
BssECI	CCNNGG	1 cut(s)	639
BssMI	GATC	8 cut(s)	70, 150, 205, 282, 538, 594, 1342, 1729
BssT1I	CCWWGG	1 cut(s)	639
Bst2UI	CCWGG	4 cut(s)	707, 811, 1619, 1686
Bst4CI	ACNGT	5 cut(s)	63, 82, 676, 686, 1224
BstAPI	GCANNNNNTGC	1 cut(s)	305
BstAUI	TGTACA	1 cut(s)	1743
BstBI	TTCGAA	1 cut(s)	919
BstC8I	GCNNGC	4 cut(s)	222, 350, 587, 1328
BstDEI	CTNAG	6 cut(s)	31, 104, 842, 964, 1032, 1628
BstF5I	GGATG	4 cut(s)	400, 483, 715, 1528
BstHHI	GCGC	1 cut(s)	663
BstKTI	GATC	8 cut(s)	73, 153, 208, 285, 541, 597, 1345, 1732
BstMAI	GTCTC	4 cut(s)	967, 1166, 1187, 1686
BstMBI	GATC	8 cut(s)	70, 150, 205, 282, 538, 594, 1342, 1729
BstMWI	GCNNNNNNNGC	4 cut(s)	131, 305, 1456, 1483
BstNI	CCWGG	4 cut(s)	707, 811, 1619, 1686
BstNSI	RCATGY	1 cut(s)	224
BstSCI	CCNGG	5 cut(s)	705, 809, 1257, 1617, 1684
BstSFI	CTRYAG	2 cut(s)	1127, 1281
BstSLI	GKGCMC	1 cut(s)	273
BstV1I	GCAGC	7 cut(s)	292, 295, 354, 357, 779, 1683, 1686
BstV2I	GAAGAC	5 cut(s)	627, 825, 891, 1206, 1521
BstX2I	RGATCY	2 cut(s)	205, 594
BstYI	RGATCY	2 cut(s)	205, 594
Bsu36I	CCTNAGG	1 cut(s)	1628
BsuI	GTATCC	1 cut(s)	417
BsuRI	GGCC	2 cut(s)	721, 993
BtgZI	GCGATG	2 cut(s)	239, 1363
BtrI	CACGTC	1 cut(s)	277
BtsCI	GGATG	4 cut(s)	400, 483, 715, 1528
BtsI	GCAGTG	1 cut(s)	596
BtsIMutI	CAGTG	6 cut(s)	160, 596, 672, 910, 972, 1231
Cac8I	GCNNGC	4 cut(s)	222, 350, 587, 1328
CaiI	CAGNNNCTG	2 cut(s)	308, 348
CfoI	GCGC	1 cut(s)	663
CseI	GACGC	1 cut(s)	50
Csp6I	GTAC	3 cut(s)	1354, 1648, 1744
CspCI	CAANNNNNGTGG	2 cut(s)	796, 831
CviAII	CATG	7 cut(s)	189, 221, 1082, 1472, 1519, 1576, 1714
CviQI	GTAC	3 cut(s)	1354, 1648, 1744
DdeI	CTNAG	6 cut(s)	31, 104, 842, 964, 1032, 1628
DpnI	GATC	8 cut(s)	72, 152, 207, 284, 540, 596, 1344, 1731
DpnII	GATC	8 cut(s)	70, 150, 205, 282, 538, 594, 1342, 1729
DraI	TTTAAA	1 cut(s)	1702
DraIII	CACNNNGTG	1 cut(s)	277
Eco130I	CCWWGG	1 cut(s)	639
Eco24I	GRGCYC	1 cut(s)	1271
Eco57I	CTGAAG	3 cut(s)	620, 1035, 1218
Eco81I	CCTNAGG	1 cut(s)	1628
EcoRII	CCWGG	4 cut(s)	705, 809, 1617, 1684
EcoT14I	CCWWGG	1 cut(s)	639
EcoT22I	ATGCAT	1 cut(s)	916
EcoT38I	GRGCYC	1 cut(s)	1271
ErhI	CCWWGG	1 cut(s)	639
Esp3I	CGTCTC	1 cut(s)	1686
FaeI	CATG	7 cut(s)	192, 224, 1085, 1475, 1522, 1579, 1717
FalI	AAGNNNNNCTT	2 cut(s)	167, 199
FaqI	GGGAC	1 cut(s)	1609
FatI	CATG	7 cut(s)	188, 220, 1081, 1471, 1518, 1575, 1713
FauI	CCCGC	3 cut(s)	225, 1389, 1411
FauNDI	CATATG	1 cut(s)	385
FbaI	TGATCA	3 cut(s)	70, 150, 282
FblI	GTMKAC	1 cut(s)	39
Fnu4HI	GCNGC	7 cut(s)	306, 309, 343, 346, 793, 1672, 1675
FokI	GGATG	4 cut(s)	407, 490, 722, 1535
FriOI	GRGCYC	1 cut(s)	1271
Fsp4HI	GCNGC	7 cut(s)	306, 309, 343, 346, 793, 1672, 1675
FspBI	CTAG	3 cut(s)	875, 942, 1541
FspI	TGCGCA	1 cut(s)	662
GlaI	GCGC	1 cut(s)	662
GluI	GCNGC	7 cut(s)	306, 309, 343, 346, 793, 1672, 1675
GsaI	CCCAGC	2 cut(s)	766, 895
GsuI	CTGGAG	2 cut(s)	1640, 1668
HaeIII	GGCC	2 cut(s)	721, 993
HapII	CCGG	2 cut(s)	209, 1258
HgaI	GACGC	1 cut(s)	50
HhaI	GCGC	1 cut(s)	663
Hin1II	CATG	7 cut(s)	192, 224, 1085, 1475, 1522, 1579, 1717
Hin6I	GCGC	1 cut(s)	661
HinP1I	GCGC	1 cut(s)	661
HincII	GTYRAC	2 cut(s)	40, 240
HindII	GTYRAC	2 cut(s)	40, 240
HindIII	AAGCTT	1 cut(s)	1021
HinfI	GANTC	5 cut(s)	524, 666, 1109, 1121, 1622
HpaII	CCGG	2 cut(s)	209, 1258
HphI	GGTGA	1 cut(s)	854
Hpy166II	GTNNAC	7 cut(s)	40, 240, 271, 365, 1318, 1744, 1746
Hpy188I	TCNGA	6 cut(s)	70, 256, 739, 1033, 1054, 1072
Hpy188III	TCNNGA	9 cut(s)	74, 85, 203, 573, 598, 875, 976, 1196, 1411
Hpy8I	GTNNAC	7 cut(s)	40, 240, 271, 365, 1318, 1744, 1746
Hpy99I	CGWCG	3 cut(s)	44, 278, 440
HpyAV	CCTTC	5 cut(s)	436, 1004, 1030, 1059, 1528
HpyCH4III	ACNGT	5 cut(s)	63, 82, 676, 686, 1224
HpyCH4IV	ACGT	4 cut(s)	267, 276, 632, 822
HpyF10VI	GCNNNNNNNGC	4 cut(s)	131, 305, 1456, 1483
HpyF3I	CTNAG	6 cut(s)	31, 104, 842, 964, 1032, 1628
HpySE526I	ACGT	4 cut(s)	267, 276, 632, 822
Hsp92II	CATG	7 cut(s)	192, 224, 1085, 1475, 1522, 1579, 1717
HspAI	GCGC	1 cut(s)	661
Ksp22I	TGATCA	3 cut(s)	70, 150, 282
Kzo9I	GATC	8 cut(s)	70, 150, 205, 282, 538, 594, 1342, 1729
LmnI	GCTCC	1 cut(s)	764
Lsp1109I	GCAGC	7 cut(s)	292, 295, 354, 357, 779, 1683, 1686
LweI	GCATC	6 cut(s)	17, 308, 804, 1204, 1260, 1446
MaeI	CTAG	3 cut(s)	875, 942, 1541
MaeII	ACGT	4 cut(s)	267, 276, 632, 822
MaeIII	GTNAC	1 cut(s)	606
MalI	GATC	8 cut(s)	72, 152, 207, 284, 540, 596, 1344, 1731
MboI	GATC	8 cut(s)	70, 150, 205, 282, 538, 594, 1342, 1729
MflI	RGATCY	2 cut(s)	205, 594
MhlI	GDGCHC	3 cut(s)	273, 761, 1271
MlyI	GAGTC	4 cut(s)	660, 1103, 1115, 1631
MmeI	TCCRAC	1 cut(s)	1617
Mph1103I	ATGCAT	1 cut(s)	916
MseI	TTAA	8 cut(s)	249, 429, 519, 621, 681, 1133, 1226, 1701
MspA1I	CMGCKG	4 cut(s)	305, 348, 792, 891
MspI	CCGG	2 cut(s)	209, 1258
MspR9I	CCNGG	5 cut(s)	707, 811, 1259, 1619, 1686
MvaI	CCWGG	4 cut(s)	707, 811, 1619, 1686
MwoI	GCNNNNNNNGC	4 cut(s)	131, 305, 1456, 1483
NciI	CCSGG	1 cut(s)	1259
NdeI	CATATG	1 cut(s)	385
NdeII	GATC	8 cut(s)	70, 150, 205, 282, 538, 594, 1342, 1729
NlaIII	CATG	7 cut(s)	192, 224, 1085, 1475, 1522, 1579, 1717
NlaIV	GGNNCC	5 cut(s)	207, 447, 596, 1502, 1616
NsbI	TGCGCA	1 cut(s)	662
NsiI	ATGCAT	1 cut(s)	916
NspI	RCATGY	1 cut(s)	224
NspV	TTCGAA	1 cut(s)	919
PaeI	GCATGC	1 cut(s)	224
PcsI	WCGNNNNNNNCGW	2 cut(s)	273, 1501
PfeI	GAWTC	1 cut(s)	524
PflFI	GACNNNGTC	1 cut(s)	1112
PflMI	CCANNNNNTGG	1 cut(s)	835
PfoI	TCCNGGA	3 cut(s)	705, 1257, 1617
PkrI	GCNGC	7 cut(s)	307, 310, 344, 347, 794, 1673, 1676
PleI	GAGTC	4 cut(s)	660, 1103, 1115, 1630
PpsI	GAGTC	4 cut(s)	660, 1103, 1115, 1630
PshBI	ATTAAT	1 cut(s)	249
PsiI	TTATAA	2 cut(s)	954, 1371
Psp6I	CCWGG	4 cut(s)	705, 809, 1617, 1684
PspFI	CCCAGC	2 cut(s)	762, 891
PspGI	CCWGG	4 cut(s)	705, 809, 1617, 1684
PspN4I	GGNNCC	5 cut(s)	207, 447, 596, 1502, 1616
PstI	CTGCAG	1 cut(s)	1131
PstNI	CAGNNNCTG	2 cut(s)	308, 348
PsuI	RGATCY	2 cut(s)	205, 594
PsyI	GACNNNGTC	1 cut(s)	1112
PvuII	CAGCTG	4 cut(s)	305, 348, 792, 891
RsaI	GTAC	3 cut(s)	1355, 1649, 1745
RsaNI	GTAC	3 cut(s)	1354, 1648, 1744
SalI	GTCGAC	1 cut(s)	38
SaqAI	TTAA	8 cut(s)	249, 429, 519, 621, 681, 1133, 1226, 1701
SatI	GCNGC	7 cut(s)	306, 309, 343, 346, 793, 1672, 1675
Sau3AI	GATC	8 cut(s)	70, 150, 205, 282, 538, 594, 1342, 1729
SchI	GAGTC	4 cut(s)	660, 1103, 1115, 1631
ScrFI	CCNGG	5 cut(s)	707, 811, 1259, 1619, 1686
SduI	GDGCHC	3 cut(s)	273, 761, 1271
SfaNI	GCATC	6 cut(s)	17, 308, 804, 1204, 1260, 1446
SfcI	CTRYAG	2 cut(s)	1127, 1281
SfuI	TTCGAA	1 cut(s)	919
SmlI	CTYRAG	1 cut(s)	1163
SmoI	CTYRAG	1 cut(s)	1163
SphI	GCATGC	1 cut(s)	224
SsiI	CCGC	5 cut(s)	218, 1151, 1217, 1396, 1418
SspI	AATATT	1 cut(s)	92
SspMI	CTAG	3 cut(s)	875, 942, 1541
StyD4I	CCNGG	5 cut(s)	705, 809, 1257, 1617, 1684
StyI	CCWWGG	1 cut(s)	639
TaaI	ACNGT	5 cut(s)	63, 82, 676, 686, 1224
TaiI	ACGT	4 cut(s)	270, 279, 635, 825
TaqI	TCGA	5 cut(s)	39, 919, 1119, 1662, 1728
TatI	WGTACW	1 cut(s)	1743
TfiI	GAWTC	1 cut(s)	524
Tru1I	TTAA	8 cut(s)	249, 429, 519, 621, 681, 1133, 1226, 1701
Tru9I	TTAA	8 cut(s)	249, 429, 519, 621, 681, 1133, 1226, 1701
TscAI	CASTG	6 cut(s)	160, 596, 679, 910, 972, 1238
TseI	GCWGC	7 cut(s)	305, 308, 342, 345, 792, 1671, 1674
TspDTI	ATGAA	9 cut(s)	135, 275, 470, 639, 666, 848, 1098, 1158, 1699
TspGWI	ACGGA	2 cut(s)	155, 1493
TspRI	CASTG	6 cut(s)	160, 596, 679, 910, 972, 1238
Tth111I	GACNNNGTC	1 cut(s)	1112
Van91I	CCANNNNNTGG	1 cut(s)	835
VneI	GTGCAC	1 cut(s)	269
VspI	ATTAAT	1 cut(s)	249
XapI	RAATTY	6 cut(s)	577, 654, 855, 1076, 1606, 1703
XbaI	TCTAGA	1 cut(s)	874
XceI	RCATGY	1 cut(s)	224
XmiI	GTMKAC	1 cut(s)	39
XspI	CTAG	3 cut(s)	875, 942, 1541
Zsp2I	ATGCAT	1 cut(s)	916

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.