RchiOBHm_Chr5g0001221

NB-ARC domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000540
Physical Location & Seq
Forward (+)
650504 .. 654103
3600 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ28267

Sequence Viewer

Length: 2529 bp
ATGTCGTTAATAGTGGAGGCTATGGTCCAGACTTTGACGGAGCAAGTGTTCACAGCTCTAATGAACCAGGCTGAATTTGCACTCGACTTCAGCGGCCAGTTTGAGCAGTTGAAGACGGGGCTTGATCTCACCAAGGCCTTACTGTCAGACACGGAGAGTCTCAACCACAAGAACAACACTATCAAGACAGCATTATTTGATCTAAGGGAAGTCATCTACAAAGCCGACGATGTACTCACTGATTGCTTGGTTAGAGATGAATACAGAAAGGAGGGATCCTGTTCCGGCTCCTTGCTTCACGATCCATTCTTCCTGCATCGTACAGGAAAGAAATTGAAAGACATCAATTTGCGTATGAAGGAGATAGAGAAGACCTTGGGCCGGTTTTTGAGGGCACCGGACAGCATCCATAGAGAAGATGCATACCAAGTTAGGGTGATGGTGTCACAGGACTGGAATCCAACTGAGATAATTGGGTTGGATAGCGATGTGGAGAAGATTAAGGGTTGGATTTTTGACACCAGCAAGGTACTTCACTATGTAGGCATTGTGGGAATGGGTGGCTTGGGCAAAACCACCATTGCTCAAAAGATATTTCATGATCTAGAAGTAGTAGGCCACTTTCACAAGCTGATATGGGTGTGTGTTTCGCAATCATTCAGCGCTGAGCGGATTATGAGGAGCATCCTAGAAGGTCTAGGAGAAAACGTCTCAGGAGCCGGTGTTACTCAGATTTTGAGCAGAATTCAGCAAGTACTTAAAGATAAGAGCTGTCTGATTGTCATGGATGATGTGTGGAATCACACAGATGTTGATTGGTGGACCAACCTCTGTTCTGTTATACCCAAACAAAGCAGCTGCATCATAGTTACAACTAGAAATGAAGAAGCTGCAATTGGTATGGGAGTTGAAAGCTCACGAATCCATCGTCCGAAGACTCTTAATGATGAGGAAAGCTGGTGTTTATTCTCCAAGTTTGCATTTCCTACAAGCAAGGGATTGTGTCCGGAGGACAGGCTTGAGAAATTAGGAAAAGAACTCTTGAAGAAGTGTGGTGGACTCCCACTCGCAATCAAGACTATAGGATCCTTGTTGGCATCAAAGATCGACTACCCTTCACAGTGGACACGAGTTCTGGAGAGCTTTCATTCTTTGACAACCGAGGGAAAAACTAGCTCACTAAGCTCTGTTATGGCCTGTTTGCGATTGAGCTATGATGAACTTCCGCCTGGTTTGAAGCAATGCCTGCTGTGTTTCTCCATCTATCCTGAAGACTACGAGATACATGCTGATCAGTTAATCTACTGGTGGATAGGAGAAGGCCTCGTGCAAAGCAAGGGCTCAAGAACAGCAGTAGAAGTTGGATATGAGTATCTCACAGTACTAATCAGCAGATGCTTGGTTGAAATTGTGCAGAGGAGAGGATTTGATGGGAGAGTCTACAAGTGCAAAATACATGATATGGTGCGGGAACTGATAATATTGATTGCAGAAGAGGAGGAATTTTGCGGCTTTGACGAACGAGGCAGACAAGAATTGACAGCAAAGACTAGGTGGTTATGCTTTATTGATGAAATGGATGAAAAGTCCTTGAAACGTAGTTCAAAGCTTAGGGCATTGTTGCTGATGTCAAGCCGCGGATTTGACTTTGAAAGGAATTCGGGGTTTCTGCTGCGATCCCTCAGAATGTTGGATTTATCAAACTGCACTGTGGATGAGCATTGTGTGGAGGATCTCTTCAGCTGGATCAGCTCCCTTAAGCGCTTAGCATCTCTGAACTTAAGTGGTATTCAAGCCCTGAAAGAATTGCCCTCTTCAATTCATAAACTCCTCAACCTCCAACTTTTGCTTTTAACAGGTTGCAGCAATCTCGTAAAAATACACCCTTCCATCACTAATTTGAAGAAGTTGATTATTTTGGACCTAGGCGGATGTCCAATCCAATACTTGCCTCAGGGATTGGGAAGGCTTTCTTATCTTCAAGAGCTCTCCGGATTCAAGGTTGTGAGTCAGCCTAGAAAGCCAAGTTTCCGGCTTCTTGAGATTAGGGAATTGATCAACCTGAGAGTACTGCGAATACATGTAAGTAGTACCACAGTTATCGCAGACAATGAACTTGATGTCCTCTCTCAGCTTAGAAGGCTCAATGTTCTAGCTATTGATGCAGAAGATTGTAGAAATAACAATGTTTTGGAAATGCTAGAAGGGCTGACCCCTCCTCCAAGCCTACAAGAGCTATATCTTAGGAATTACAAGAAGGAAACTCTGCCCAACTGGGTTAATCCTGGACAACTTTCACGATTGCAGTATCTTTGCATCGAGAATGGAGACCTTGTCAAGCTAAGCAGTGGTCAAACCACCTGGAATCTTGAAGGCCTGTGTCTCAAGTACTTGATGAGGCTAGAGGTAGACTGGAACGACTTGGAGAAGGATATGCCTGTACTTCACTACATGGAGATCAGTCATTGTTACAACTTGACGGATTTCCCTTGTTCGGTTATGGAACCTGGGGTCTGGAGAAAGCATTAA

Protein Analysis

842

Amino Acids

95.89

Weight (kDa)

6.12

Isoelectric Point (pI)

42.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 8 - 94 7.8e-13 Rx N-terminal domain
NB-ARC PF00931 162 - 331 2.9e-40 NB-ARC domain
AAA_22 PF13401 183 - 265 5.3e-06 AAA domain
WHD_DRP PF23559 421 - 493 6.5e-19 Disease resistance protein Winged helix domain
LRR_14 PF23598 559 - 774 3.5e-28 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 694 - 783 8.1e-09 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000540)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G50950 AT3G50950
fragaria_vesca FvH4_1g12710 FvH4_1g12710 FvH4_3g00820 FvH4_3g00820 FvH4_3g00820 FvH4_3g00820
malus_domestica MD02G1141100.v1.1 MD10G1333100.v1.1
prunus_persica Prupe.4G009300_v2.0.a1 Prupe.4G009300_v2.0.a1 Prupe.4G009300_v2.0.a1 Prupe.6G150700_v2.0.a1 Prupe.7G160100_v2.0.a1 Prupe.7G160100_v2.0.a1
pyrus_communis pycom02g11110 pycom10g28240 pycom10g28340 pycom10g28360 pycom10g28450
rosa_chinensis RchiOBHm_Chr2g0100711 RchiOBHm_Chr3g0485861 RchiOBHm_Chr4g0391451 RchiOBHm_Chr4g0391591 RchiOBHm_Chr4g0391601 RchiOBHm_Chr4g0391681 RchiOBHm_Chr5g0001141 RchiOBHm_Chr5g0001221
rosa_laevigata RLG00000017001
rosa_multiflora Rmu_co8260007.1_g000001 Rmu_co8260103.1_g000001 Rmu_co8519155.1_g000001 Rmu_sc0000060.1_g000050 Rmu_sc0000255.1_g000036 Rmu_sc0001174.1_g000023 Rmu_sc0001174.1_g000025 Rmu_sc0001659.1_g000009 Rmu_sc0001706.1_g000009 Rmu_sc0001706.1_g000024 Rmu_sc0001706.1_g000035 Rmu_sc0004189.1_g000018 Rmu_sc0004189.1_g000030 Rmu_sc0004862.1_g000002 Rmu_sc0004876.1_g000038 Rmu_sc0023741.1_g000004
rosa_roxburghii Rroxscaffold_1G00075100 Rroxscaffold_1G00075160 Rroxscaffold_1G00075210 Rroxscaffold_5G00337160 Rroxscaffold_5G00386590 Rroxscaffold_6G00396300
rosa_rugosa Rorug03G0336200 Rorug03G0336300 Rorug04G0389400
rosa_samantha Rh2AG140400 Rh2BG144100 Rh2CG145400 Rh2DG144900 Rh3AG271200 Rh4AG038000 Rh4AG038700 Rh4BG032000 Rh4BG032800 Rh4CG041100 Rh4CG041300 Rh4DG035500 Rh4DG035600 Rh5AG008800 Rh5AG009100 Rh5BG010900 Rh5BG011500 Rh5BG011600 Rh5BG012000 Rh5CG009400 Rh5CG010000 Rh5DG009800
rosa_wichuraiana Rw2G010940 Rw4G002950 Rw4G002990 Rw4G003000 Rw5G000880 Rw5G000950 Rw5G000960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 394
AccBSI CCGCTC 1 cut(s) 670
AccI GTMKAC 2 cut(s) 1440, 2409
AccII CGCG 1 cut(s) 1638
AccIII TCCGGA 2 cut(s) 1006, 1991
AciI CCGC 8 cut(s) 93, 670, 1226, 1468, 1509, 1636, 1638, 1929
AclWI GGATC 8 cut(s) 270, 283, 296, 1080, 1093, 1671, 1740, 1754
AcoI YGGCCR 1 cut(s) 94
AcsI RAATTY 4 cut(s) 74, 744, 1502, 1657
AcuI CTGAAG 3 cut(s) 73, 1290, 1723
AfaI GTAC 9 cut(s) 234, 322, 531, 756, 1383, 2070, 2092, 2390, 2442
AfeI AGCGCT 2 cut(s) 664, 1763
AfiI CCNNNNNNNGG 3 cut(s) 381, 433, 2494
AflII CTTAAG 2 cut(s) 1757, 1780
AflIII ACRYGT 1 cut(s) 2080
AjnI CCWGG 5 cut(s) 66, 1228, 2284, 2360, 2506
AjuI GAANNNNNNNTTGG 2 cut(s) 879, 911
AloI GAACNNNNNNTCC 4 cut(s) 32, 64, 2106, 2138
Alw21I GWGCWC 1 cut(s) 1989
Alw26I GTCTC 4 cut(s) 164, 715, 2322, 2387
AlwI GGATC 8 cut(s) 270, 283, 296, 1080, 1093, 1671, 1740, 1754
Aor13HI TCCGGA 2 cut(s) 1006, 1991
Aor51HI AGCGCT 2 cut(s) 664, 1763
AoxI GGCC 7 cut(s) 94, 135, 379, 616, 1194, 1321, 2374
ApeKI GCWGC 5 cut(s) 855, 858, 890, 1672, 1863
ApoI RAATTY 4 cut(s) 74, 744, 1502, 1657
ArsI GACNNNNNNTTYG 2 cut(s) 913, 945
Asp700I GAANNNNTTC 1 cut(s) 1969
AspA2I CCTAGG 1 cut(s) 1924
AspLEI GCGC 2 cut(s) 665, 1764
AspS9I GGNCC 4 cut(s) 25, 379, 822, 1921
AsuHPI GGTGA 2 cut(s) 121, 448
AvaII GGWCC 3 cut(s) 25, 822, 1921
AvrII CCTAGG 1 cut(s) 1924
AxyI CCTNAGG 1 cut(s) 1953
BaeGI GKGCMC 1 cut(s) 397
BamHI GGATCC 2 cut(s) 275, 1085
BanI GGYRCC 1 cut(s) 394
BanII GRGCYC 2 cut(s) 1343, 1989
BauI CACGAG 2 cut(s) 1128, 1325
BbsI GAAGAC 4 cut(s) 119, 377, 941, 1278
Bbv12I GWGCWC 1 cut(s) 1989
BbvI GCAGC 5 cut(s) 845, 867, 877, 1659, 1875
BccI CCATC 5 cut(s) 433, 933, 1268, 1424, 1898
BcgI CGANNNNNNTGC 4 cut(s) 1268, 1302, 1852, 1886
BciT130I CCWGG 5 cut(s) 68, 1230, 2286, 2362, 2508
BclI TGATCA 2 cut(s) 1291, 2055
BcoDI GTCTC 4 cut(s) 164, 715, 2322, 2387
BfmI CTRYAG 1 cut(s) 1080
BfoI RGCGCY 2 cut(s) 666, 1765
BfrI CTTAAG 2 cut(s) 1757, 1780
BisI GCNGC 8 cut(s) 94, 856, 859, 891, 1510, 1636, 1673, 1864
BlnI CCTAGG 1 cut(s) 1924
BlpI GCTNAGC 3 cut(s) 666, 1765, 2342
BlsI GCNGC 8 cut(s) 95, 857, 860, 892, 1511, 1637, 1674, 1865
BmcAI AGTACT 4 cut(s) 756, 1383, 2070, 2390
Bme1390I CCNGG 5 cut(s) 68, 1230, 2286, 2362, 2508
Bme18I GGWCC 3 cut(s) 25, 822, 1921
BmgT120I GGNCC 4 cut(s) 25, 379, 822, 1921
BmiI GGNNCC 6 cut(s) 277, 289, 396, 718, 1087, 2505
BmrFI CCNGG 5 cut(s) 68, 1230, 2286, 2362, 2508
BmrI ACTGGG 1 cut(s) 2284
BmuI ACTGGG 1 cut(s) 2284
BpiI GAAGAC 4 cut(s) 119, 377, 941, 1278
BplI GAGNNNNNCTC 2 cut(s) 1308, 1340
BpmI CTGGAG 1 cut(s) 1157
Bpu10I CCTNAGC 1 cut(s) 1610
Bpu1102I GCTNAGC 3 cut(s) 666, 1765, 2342
BpuEI CTTGAG 4 cut(s) 1040, 1327, 2060, 2369
BsaI GGTCTC 1 cut(s) 2322
BsaJI CCNNGG 6 cut(s) 132, 375, 1161, 1636, 1924, 2507
BsaWI WCCGGW 3 cut(s) 397, 1006, 1991
Bsc4I CCNNNNNNNGG 3 cut(s) 381, 433, 2494
Bse118I RCCGGY 2 cut(s) 381, 719
Bse1I ACTGG 5 cut(s) 97, 458, 1310, 2279, 2417
Bse21I CCTNAGG 1 cut(s) 1953
Bse3DI GCAATG 2 cut(s) 579, 1247
BseAI TCCGGA 2 cut(s) 1006, 1991
BseBI CCWGG 5 cut(s) 68, 1230, 2286, 2362, 2508
BseDI CCNNGG 6 cut(s) 132, 375, 1161, 1636, 1924, 2507
BseGI GGATG 6 cut(s) 405, 684, 793, 1585, 1720, 1937
BseLI CCNNNNNNNGG 3 cut(s) 381, 433, 2494
BseMI GCAATG 2 cut(s) 579, 1247
BseMII CTCAG 8 cut(s) 456, 657, 726, 743, 1696, 1967, 2054, 2144
BseNI ACTGG 5 cut(s) 97, 458, 1310, 2279, 2417
BseRI GAGGAG 5 cut(s) 694, 1432, 1511, 1820, 2208
BseSI GKGCMC 1 cut(s) 397
BseXI GCAGC 5 cut(s) 845, 867, 877, 1659, 1875
BsgI GTGCAG 2 cut(s) 1433, 1690
Bsh1236I CGCG 1 cut(s) 1638
BshFI GGCC 7 cut(s) 96, 137, 381, 618, 1196, 1323, 2376
BshNI GGYRCC 1 cut(s) 394
BsiHKAI GWGCWC 1 cut(s) 1989
BsiSI CCGG 7 cut(s) 285, 382, 398, 720, 1007, 1992, 2032
BslI CCNNNNNNNGG 3 cut(s) 381, 433, 2494
BsmAI GTCTC 4 cut(s) 164, 715, 2322, 2387
BsmBI CGTCTC 1 cut(s) 715
BsnI GGCC 7 cut(s) 96, 137, 381, 618, 1196, 1323, 2376
Bso31I GGTCTC 1 cut(s) 2322
Bsp1286I GDGCHC 3 cut(s) 397, 1343, 1989
Bsp13I TCCGGA 2 cut(s) 1006, 1991
Bsp1720I GCTNAGC 3 cut(s) 666, 1765, 2342
BspACI CCGC 8 cut(s) 93, 670, 1226, 1468, 1509, 1636, 1638, 1929
BspANI GGCC 7 cut(s) 96, 137, 381, 618, 1196, 1323, 2376
BspCNI CTCAG 8 cut(s) 457, 658, 725, 742, 1695, 1966, 2055, 2143
BspEI TCCGGA 2 cut(s) 1006, 1991
BspFNI CGCG 1 cut(s) 1638
BspHI TCATGA 1 cut(s) 598
BspLI GGNNCC 6 cut(s) 277, 289, 396, 718, 1087, 2505
BspPI GGATC 8 cut(s) 270, 283, 296, 1080, 1093, 1671, 1740, 1754
BspT107I GGYRCC 1 cut(s) 394
BspTI CTTAAG 2 cut(s) 1757, 1780
BspTNI GGTCTC 1 cut(s) 2322
BsrBI CCGCTC 1 cut(s) 670
BsrDI GCAATG 2 cut(s) 579, 1247
BsrFI RCCGGY 2 cut(s) 381, 719
BsrI ACTGG 5 cut(s) 97, 458, 1310, 2279, 2417
BssAI RCCGGY 2 cut(s) 381, 719
BssECI CCNNGG 6 cut(s) 132, 375, 1161, 1636, 1924, 2507
BssSI CACGAG 2 cut(s) 1128, 1325
BssT1I CCWWGG 3 cut(s) 132, 375, 1924
Bst2BI CACGAG 2 cut(s) 1128, 1325
Bst2UI CCWGG 5 cut(s) 68, 1230, 2286, 2362, 2508
Bst4CI ACNGT 5 cut(s) 144, 1122, 1381, 1711, 2098
Bst6I CTCTTC 3 cut(s) 1488, 1742, 1819
BstAFI CTTAAG 2 cut(s) 1757, 1780
BstAPI GCANNNNNTGC 1 cut(s) 1246
BstC8I GCNNGC 1 cut(s) 1247
BstDSI CCRYGG 1 cut(s) 1636
BstF5I GGATG 6 cut(s) 405, 684, 793, 1585, 1720, 1937
BstFNI CGCG 1 cut(s) 1638
BstH2I RGCGCY 2 cut(s) 666, 1765
BstHHI GCGC 2 cut(s) 665, 1764
BstMAI GTCTC 4 cut(s) 164, 715, 2322, 2387
BstMWI GCNNNNNNNGC 8 cut(s) 77, 1182, 1246, 1749, 2020, 2140, 2162, 2206
BstNI CCWGG 5 cut(s) 68, 1230, 2286, 2362, 2508
BstNSI RCATGY 2 cut(s) 1289, 2084
BstSCI CCNGG 5 cut(s) 66, 1228, 2284, 2360, 2506
BstSFI CTRYAG 1 cut(s) 1080
BstSLI GKGCMC 1 cut(s) 397
BstUI CGCG 1 cut(s) 1638
BstV1I GCAGC 5 cut(s) 845, 867, 877, 1659, 1875
BstV2I GAAGAC 4 cut(s) 119, 377, 941, 1278
BstX2I RGATCY 3 cut(s) 275, 1085, 1732
BstYI RGATCY 3 cut(s) 275, 1085, 1732
Bsu36I CCTNAGG 1 cut(s) 1953
BsuRI GGCC 7 cut(s) 96, 137, 381, 618, 1196, 1323, 2376
BtgI CCRYGG 1 cut(s) 1636
BtgZI GCGATG 1 cut(s) 501
BtsCI GGATG 6 cut(s) 405, 684, 793, 1585, 1720, 1937
BtsI GCAGTG 1 cut(s) 2353
BtsIMutI CAGTG 4 cut(s) 237, 1127, 1707, 2353
Cac8I GCNNGC 1 cut(s) 1247
CciI TCATGA 1 cut(s) 598
CfoI GCGC 2 cut(s) 665, 1764
Cfr10I RCCGGY 2 cut(s) 381, 719
Cfr13I GGNCC 4 cut(s) 25, 379, 822, 1921
Cfr42I CCGCGG 1 cut(s) 1639
Csp6I GTAC 9 cut(s) 233, 321, 530, 755, 1382, 2069, 2091, 2389, 2441
CviAII CATG 6 cut(s) 599, 784, 1286, 1457, 2081, 2452
CviQI GTAC 9 cut(s) 233, 321, 530, 755, 1382, 2069, 2091, 2389, 2441
EaeI YGGCCR 1 cut(s) 94
Eam1104I CTCTTC 3 cut(s) 1488, 1742, 1819
EarI CTCTTC 3 cut(s) 1488, 1742, 1819
EciI GGCGGA 2 cut(s) 1215, 1944
Ecl136II GAGCTC 1 cut(s) 1987
Eco130I CCWWGG 3 cut(s) 132, 375, 1924
Eco147I AGGCCT 3 cut(s) 137, 1323, 2376
Eco24I GRGCYC 2 cut(s) 1343, 1989
Eco31I GGTCTC 1 cut(s) 2322
Eco47I GGWCC 3 cut(s) 25, 822, 1921
Eco47III AGCGCT 2 cut(s) 664, 1763
Eco53kI GAGCTC 1 cut(s) 1987
Eco57I CTGAAG 3 cut(s) 73, 1290, 1723
Eco81I CCTNAGG 1 cut(s) 1953
EcoICRI GAGCTC 1 cut(s) 1987
EcoRI GAATTC 2 cut(s) 744, 1657
EcoRII CCWGG 5 cut(s) 66, 1228, 2284, 2360, 2506
EcoT14I CCWWGG 3 cut(s) 132, 375, 1924
EcoT22I ATGCAT 1 cut(s) 424
EcoT38I GRGCYC 2 cut(s) 1343, 1989
ErhI CCWWGG 3 cut(s) 132, 375, 1924
Esp3I CGTCTC 1 cut(s) 715
FaeI CATG 6 cut(s) 602, 787, 1289, 1460, 2084, 2455
FalI AAGNNNNNCTT 2 cut(s) 1957, 1989
FatI CATG 6 cut(s) 598, 783, 1285, 1456, 2080, 2451
FauI CCCGC 1 cut(s) 1461
FbaI TGATCA 2 cut(s) 1291, 2055
FblI GTMKAC 2 cut(s) 1440, 2409
Fnu4HI GCNGC 8 cut(s) 94, 856, 859, 891, 1510, 1636, 1673, 1864
FokI GGATG 6 cut(s) 392, 671, 800, 1592, 1727, 1944
FriOI GRGCYC 2 cut(s) 1343, 1989
Fsp4HI GCNGC 8 cut(s) 94, 856, 859, 891, 1510, 1636, 1673, 1864
GlaI GCGC 2 cut(s) 664, 1763
GluI GCNGC 8 cut(s) 94, 856, 859, 891, 1510, 1636, 1673, 1864
GsuI CTGGAG 1 cut(s) 1157
HaeII RGCGCY 2 cut(s) 666, 1765
HaeIII GGCC 7 cut(s) 96, 137, 381, 618, 1196, 1323, 2376
HapII CCGG 7 cut(s) 285, 382, 398, 720, 1007, 1992, 2032
HhaI GCGC 2 cut(s) 665, 1764
Hin1II CATG 6 cut(s) 602, 787, 1289, 1460, 2084, 2455
Hin6I GCGC 2 cut(s) 663, 1762
HinP1I GCGC 2 cut(s) 663, 1762
HindIII AAGCTT 1 cut(s) 1607
HpaII CCGG 7 cut(s) 285, 382, 398, 720, 1007, 1992, 2032
HphI GGTGA 2 cut(s) 121, 448
Hpy166II GTNNAC 6 cut(s) 51, 822, 1058, 1125, 1441, 2410
Hpy188I TCNGA 6 cut(s) 148, 732, 777, 933, 1685, 1776
Hpy8I GTNNAC 6 cut(s) 51, 822, 1058, 1125, 1441, 2410
Hpy99I CGWCG 1 cut(s) 230
HpyCH4III ACNGT 5 cut(s) 144, 1122, 1381, 1711, 2098
HpyCH4IV ACGT 2 cut(s) 708, 1597
HpyF10VI GCNNNNNNNGC 8 cut(s) 77, 1182, 1246, 1749, 2020, 2140, 2162, 2206
HpySE526I ACGT 2 cut(s) 708, 1597
Hsp92II CATG 6 cut(s) 602, 787, 1289, 1460, 2084, 2455
HspAI GCGC 2 cut(s) 663, 1762
Kpn2I TCCGGA 2 cut(s) 1006, 1991
Ksp22I TGATCA 2 cut(s) 1291, 2055
KspI CCGCGG 1 cut(s) 1639
LmnI GCTCC 5 cut(s) 40, 293, 681, 716, 1757
Lsp1109I GCAGC 5 cut(s) 845, 867, 877, 1659, 1875
MaeII ACGT 2 cut(s) 708, 1597
MaeIII GTNAC 4 cut(s) 444, 724, 868, 2468
MbiI CCGCTC 1 cut(s) 670
MfeI CAATTG 1 cut(s) 894
MflI RGATCY 3 cut(s) 275, 1085, 1732
MhlI GDGCHC 3 cut(s) 397, 1343, 1989
MlyI GAGTC 5 cut(s) 166, 931, 1053, 1446, 2017
MmeI TCCRAC 6 cut(s) 459, 485, 488, 1342, 1671, 1864
Mph1103I ATGCAT 1 cut(s) 424
MroI TCCGGA 2 cut(s) 1006, 1991
MroXI GAANNNNTTC 1 cut(s) 1969
MslI CAYNNNNRTG 1 cut(s) 807
MspA1I CMGCKG 4 cut(s) 93, 858, 1638, 1743
MspCI CTTAAG 2 cut(s) 1757, 1780
MspI CCGG 7 cut(s) 285, 382, 398, 720, 1007, 1992, 2032
MspR9I CCNGG 5 cut(s) 68, 1230, 2286, 2362, 2508
MunI CAATTG 1 cut(s) 894
MvaI CCWGG 5 cut(s) 68, 1230, 2286, 2362, 2508
MvnI CGCG 1 cut(s) 1638
MwoI GCNNNNNNNGC 8 cut(s) 77, 1182, 1246, 1749, 2020, 2140, 2162, 2206
NlaIII CATG 6 cut(s) 602, 787, 1289, 1460, 2084, 2455
NlaIV GGNNCC 6 cut(s) 277, 289, 396, 718, 1087, 2505
NmuCI GTSAC 1 cut(s) 444
NsiI ATGCAT 1 cut(s) 424
NspI RCATGY 2 cut(s) 1289, 2084
PagI TCATGA 1 cut(s) 598
PceI AGGCCT 3 cut(s) 137, 1323, 2376
PciI ACATGT 1 cut(s) 2080
PcsI WCGNNNNNNNCGW 1 cut(s) 925
PdmI GAANNNNTTC 1 cut(s) 1969
PfeI GAWTC 5 cut(s) 457, 799, 921, 1995, 2365
PflFI GACNNNGTC 1 cut(s) 2333
PfoI TCCNGGA 1 cut(s) 2284
PkrI GCNGC 8 cut(s) 95, 857, 860, 892, 1511, 1637, 1674, 1865
PleI GAGTC 5 cut(s) 165, 931, 1053, 1445, 2016
PpsI GAGTC 5 cut(s) 165, 931, 1053, 1445, 2016
PscI ACATGT 1 cut(s) 2080
Psp124BI GAGCTC 1 cut(s) 1989
Psp6I CCWGG 5 cut(s) 66, 1228, 2284, 2360, 2506
PspGI CCWGG 5 cut(s) 66, 1228, 2284, 2360, 2506
PspN4I GGNNCC 6 cut(s) 277, 289, 396, 718, 1087, 2505
PspPI GGNCC 4 cut(s) 25, 379, 822, 1921
PsuI RGATCY 3 cut(s) 275, 1085, 1732
PsyI GACNNNGTC 1 cut(s) 2333
PvuII CAGCTG 2 cut(s) 858, 1743
RsaI GTAC 9 cut(s) 234, 322, 531, 756, 1383, 2070, 2092, 2390, 2442
RsaNI GTAC 9 cut(s) 233, 321, 530, 755, 1382, 2069, 2091, 2389, 2441
RseI CAYNNNNRTG 1 cut(s) 807
SacI GAGCTC 1 cut(s) 1989
SacII CCGCGG 1 cut(s) 1639
SatI GCNGC 8 cut(s) 94, 856, 859, 891, 1510, 1636, 1673, 1864
Sau96I GGNCC 4 cut(s) 25, 379, 822, 1921
ScaI AGTACT 4 cut(s) 756, 1383, 2070, 2390
SchI GAGTC 5 cut(s) 166, 931, 1053, 1446, 2017
ScrFI CCNGG 5 cut(s) 68, 1230, 2286, 2362, 2508
SduI GDGCHC 3 cut(s) 397, 1343, 1989
SfcI CTRYAG 1 cut(s) 1080
Sfr303I CCGCGG 1 cut(s) 1639
SgrBI CCGCGG 1 cut(s) 1639
SinI GGWCC 3 cut(s) 25, 822, 1921
SmiMI CAYNNNNRTG 1 cut(s) 807
SmlI CTYRAG 6 cut(s) 1019, 1342, 1757, 1780, 2039, 2384
SmoI CTYRAG 6 cut(s) 1019, 1342, 1757, 1780, 2039, 2384
SseBI AGGCCT 3 cut(s) 137, 1323, 2376
SsiI CCGC 8 cut(s) 93, 670, 1226, 1468, 1509, 1636, 1638, 1929
SspI AATATT 1 cut(s) 1482
SstI GAGCTC 1 cut(s) 1989
StuI AGGCCT 3 cut(s) 137, 1323, 2376
StyD4I CCNGG 5 cut(s) 66, 1228, 2284, 2360, 2506
StyI CCWWGG 3 cut(s) 132, 375, 1924
TaaI ACNGT 5 cut(s) 144, 1122, 1381, 1711, 2098
TaiI ACGT 2 cut(s) 711, 1600
TaqI TCGA 3 cut(s) 84, 1107, 2319
TatI WGTACW 6 cut(s) 232, 754, 1381, 2068, 2388, 2440
TauI GCSGC 3 cut(s) 96, 1512, 1638
TfiI GAWTC 5 cut(s) 457, 799, 921, 1995, 2365
TscAI CASTG 4 cut(s) 244, 1127, 1714, 2353
TseFI GTSAC 1 cut(s) 444
TseI GCWGC 5 cut(s) 855, 858, 890, 1672, 1863
Tsp45I GTSAC 1 cut(s) 444
TspGWI ACGGA 3 cut(s) 53, 167, 2495
TspRI CASTG 4 cut(s) 244, 1127, 1714, 2353
Tth111I GACNNNGTC 1 cut(s) 2333
Vha464I CTTAAG 2 cut(s) 1757, 1780
VpaK11BI GGWCC 3 cut(s) 25, 822, 1921
XapI RAATTY 4 cut(s) 74, 744, 1502, 1657
XbaI TCTAGA 1 cut(s) 604
XceI RCATGY 2 cut(s) 1289, 2084
XmaJI CCTAGG 1 cut(s) 1924
XmiI GTMKAC 2 cut(s) 1440, 2409
XmnI GAANNNNTTC 1 cut(s) 1969
ZrmI AGTACT 4 cut(s) 756, 1383, 2070, 2390
Zsp2I ATGCAT 1 cut(s) 424
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.