Rroxscaffold_5G00337160

NB-ARC domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0000540
Physical Location & Seq
Forward (+)
5204353 .. 5207718
3366 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00337160.1

Sequence Viewer

Length: 2508 bp
ATGTCATTAATAGTGGGGGCTATGGTCCAGACTCTAACGGAGCACGTGTTCACAGCTCTAATGAACCAGGCTGAATTTGCACTTGACTTCAGCGGCCAATTTGAGCAGTTGAAGACAGGGCTTGATCTCACGAAGGCCTTACTTGCAGACACGGAGAGTCTCAACCACAAGAACAACATTGTCAAGGCAGCATTATTTGAGCTGAGAGAAGTCATCTACAAGGCCGACGATGTACTCACTGATTGCTTGGTCAGAGATGAATACAGAAAGGATGGATCTTGTTCCGGCTCCTTGTTTCACGATCCATTCTTCCTGCATCGTACGGGAAAGAAATTGAAAGATATCAATTTGCGTATGAAGGAGATAGAGCAGACCTTGGGCAAGTTTTTGAGGGCTCCTGATAACATCCATAGAGAAGATGCCTACCAAGTTAGGGTAATGGTTTCACAGGACTGGAATCCAACTGAGATAATTGGGTTGGATAGCGATGTGGAGAAGATTAAGGGTTGGCTTTTCGACACTAGCCACGTACCCCACATAGGCATTGTGGGAATGGGAGGCTTGGGGAAAACCACCATTGCTCAAAAGATATTTCATGATCTAGAAGTTGTAGGCCACTTTCACAAGCTGATATGGGTGTGTGTTTCGCAATCATTCAGTGCTGATCGGATTATGAGGAGCATCCTAGAAGGTCTAGGAGAAAATGCCTCCGGAGCTGGTGTTACTCAGATTTTGAGCAGAATTCAGCAAGTACTTAAAGATAAGAGCTGTCTGATTGTCATGGATGATGTGTGGAATCAAACAGATGTTGATTGGTGGACCAACCTCTGCTCTGTTATACCCAAACAAAGCAGCTGCATCATCGTTACAACTAGAAATGAAGAAGCTGTAATTGGTATGGGAGTTGAAAGCTCACGAATCCACCGTCCCAAGACTCTTAATGATGAGGAAAGCTGGCGCTTGTTCTCCAAGTTTGCATTTCCTACTAGCAAGGGATTGTGCCCGGAGGACAGGCTTGAGAAATTAGGAAAAGAACTCTTGAAGAAGTGTGGTGGACTCCCACTCGCAATCAAGACTATAGGATCCTTGTTGGCATCAAAGGTCGACTCCCCTTCTCAGTGGAGACGAGTTCTGGAGAGCTTTCATGCTTTGACAACAGAGGGAAAAACTAGCTCAGTTATGGCCTCTTTGCGATTGAGCTATGATGAACTCCCGCCTGGTCTGAAGCAATGCCTGCTGTGTTTCTCCATCTATCCTGAAGACTATGAGATACGTGCTGATCAGTTAATCCATTGGTGGATTGGAGAAGGCCTCGTGCAAAGCAAGGGTTCAAGAACAGCAGTAGAAGTGGGATATGAGTATCTTGCGGAACTGATAAGCAGATGCTTGGTTGAAATTGTGCAGCAGAGAGGATTTGATGGGAGAGTCTACAAGTGCAAGATTCATGATATGGTCCGGGAACTGATAATAATGATTGCAGAGGAGGAGGAATTATGCGGCTTTGACGAACAAGGCAGACAGAAATTGACAGCAAATACTAGGTGGTTATGCTTTATGGATGAAATGGAGGAGAAGTCCTTGAAACGTAGTTCAAAGCTCAGGGCATTGTTGATGATGTCAAGCCGCAGATTTGATTTTGAAAGGAATTCTCTGCGATCCCTCAGAATGTTGGATTTATCAAACTGCACTGTGGATGAGCATTGTGTGAAGGATCTCTTCAGCTGGATCAGCTCCCTTAAGCGCTTAGCATCTTTGAACTTAAGTGGCATTCAGGCTCTGAAAGAAGTGCCATCTTCAATTCATAAACTCCTCAACCTCCAACTTTTACTTCTAACAGGTTGCAGCAATCTAGAAAAAATACACCCTTCCATCACTAATTTGAAGAAGCTGATTATTTTGGACCTAGGCGGGTGTCCAATCCAATACTTACCTCAGGGATTGGGAAGGCTTTTTTATCTTCAAGAGTTCTCTGGATTCAAGATTGTGAGTCAGCCTAGAATGCCATGTTTCCGGCTTCTTGAGATTAGGGAACTGATCAACCTGAGAGTACTGCGAATACATGTAAGCAGTACCACAGTTATTGCAGACAATGAACTTGATGTCCTCTCTCAGCTTAGAAGGCTCAATGTTCTGGCTATCGACGCAGAAGATTGTAGAAATAACAATGTTTTGGAAATGCTAGAAAGGCTGACCCCTCCTCCAAGCCTCCAAGAGTTGTATCTTAGGAATTACAAGAAAGAAACTCTGCCTAGTTGGGTTAATCCCGGACAGCTTTCAAGATTGCAGTATCTTTGCATTGAGAATGGAGACCTAGTCAAGCTAAGCAGTGGTCAAACCACCTGGAACCTTGAAGGCCTGTGTCTCAAGTACTTGATGAGGCTAGAGGTAGACTGGAAGGACTTGGAGAAGGATATGCCTGTACTCCACTACATGGAGGTCAGTCATTGTTACAACCTGAAGGATTTCCCATGTTCAGTTATGGAACCTGGGGTCTGGAGAAAGATTTAA

Protein Analysis

835

Amino Acids

95.28

Weight (kDa)

6.41

Isoelectric Point (pI)

43.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 8 - 93 1.7e-12 Rx N-terminal domain
NB-ARC PF00931 162 - 330 5.5e-41 NB-ARC domain
AAA_22 PF13401 180 - 264 1.6e-06 AAA domain
WHD_DRP PF23559 417 - 489 2.2e-20 Disease resistance protein Winged helix domain
LRR_14 PF23598 546 - 767 2.6e-26 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 687 - 776 1.6e-08 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000540)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G50950 AT3G50950
fragaria_vesca FvH4_1g12710 FvH4_1g12710 FvH4_3g00820 FvH4_3g00820 FvH4_3g00820 FvH4_3g00820
malus_domestica MD02G1141100.v1.1 MD10G1333100.v1.1
prunus_persica Prupe.4G009300_v2.0.a1 Prupe.4G009300_v2.0.a1 Prupe.4G009300_v2.0.a1 Prupe.6G150700_v2.0.a1 Prupe.7G160100_v2.0.a1 Prupe.7G160100_v2.0.a1
pyrus_communis pycom02g11110 pycom10g28240 pycom10g28340 pycom10g28360 pycom10g28450
rosa_chinensis RchiOBHm_Chr2g0100711 RchiOBHm_Chr3g0485861 RchiOBHm_Chr4g0391451 RchiOBHm_Chr4g0391591 RchiOBHm_Chr4g0391601 RchiOBHm_Chr4g0391681 RchiOBHm_Chr5g0001141 RchiOBHm_Chr5g0001221
rosa_laevigata RLG00000017001
rosa_multiflora Rmu_co8260007.1_g000001 Rmu_co8260103.1_g000001 Rmu_co8519155.1_g000001 Rmu_sc0000060.1_g000050 Rmu_sc0000255.1_g000036 Rmu_sc0001174.1_g000023 Rmu_sc0001174.1_g000025 Rmu_sc0001659.1_g000009 Rmu_sc0001706.1_g000009 Rmu_sc0001706.1_g000024 Rmu_sc0001706.1_g000035 Rmu_sc0004189.1_g000018 Rmu_sc0004189.1_g000030 Rmu_sc0004862.1_g000002 Rmu_sc0004876.1_g000038 Rmu_sc0023741.1_g000004
rosa_roxburghii Rroxscaffold_1G00075100 Rroxscaffold_1G00075160 Rroxscaffold_1G00075210 Rroxscaffold_5G00337160 Rroxscaffold_5G00386590 Rroxscaffold_6G00396300
rosa_rugosa Rorug03G0336200 Rorug03G0336300 Rorug04G0389400
rosa_samantha Rh2AG140400 Rh2BG144100 Rh2CG145400 Rh2DG144900 Rh3AG271200 Rh4AG038000 Rh4AG038700 Rh4BG032000 Rh4BG032800 Rh4CG041100 Rh4CG041300 Rh4DG035500 Rh4DG035600 Rh5AG008800 Rh5AG009100 Rh5BG010900 Rh5BG011500 Rh5BG011600 Rh5BG012000 Rh5CG009400 Rh5CG010000 Rh5DG009800
rosa_wichuraiana Rw2G010940 Rw4G002950 Rw4G002990 Rw4G003000 Rw5G000880 Rw5G000950 Rw5G000960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 2431
AccI GTMKAC 3 cut(s) 1104, 1428, 2388
AccIII TCCGGA 1 cut(s) 710
AciI CCGC 6 cut(s) 93, 1214, 1367, 1497, 1624, 1908
AclWI GGATC 7 cut(s) 283, 296, 1077, 1090, 1650, 1719, 1733
AcoI YGGCCR 1 cut(s) 94
AcsI RAATTY 3 cut(s) 74, 741, 1645
AcuI CTGAAG 5 cut(s) 73, 1244, 1278, 1702, 2477
AcvI CACGTG 1 cut(s) 46
AfaI GTAC 8 cut(s) 234, 322, 531, 753, 2049, 2071, 2369, 2421
AfeI AGCGCT 1 cut(s) 1742
AfiI CCNNNNNNNGG 3 cut(s) 433, 539, 2431
AflII CTTAAG 2 cut(s) 1736, 1759
AflIII ACRYGT 2 cut(s) 45, 2059
AjnI CCWGG 4 cut(s) 66, 1216, 2339, 2485
AjuI GAANNNNNNNTTGG 4 cut(s) 876, 908, 1812, 1844
AloI GAACNNNNNNTCC 4 cut(s) 32, 64, 2085, 2117
Alw21I GWGCWC 1 cut(s) 45
Alw26I GTCTC 4 cut(s) 164, 1117, 2301, 2366
AlwI GGATC 7 cut(s) 283, 296, 1077, 1090, 1650, 1719, 1733
AlwNI CAGNNNCTG 1 cut(s) 1777
Aor13HI TCCGGA 1 cut(s) 710
Aor51HI AGCGCT 1 cut(s) 1742
AoxI GGCC 7 cut(s) 94, 135, 222, 613, 1182, 1309, 2353
ApeKI GCWGC 5 cut(s) 188, 852, 855, 1402, 1842
ApoI RAATTY 3 cut(s) 74, 741, 1645
ArsI GACNNNNNNTTYG 2 cut(s) 910, 942
AseI ATTAAT 1 cut(s) 8
Asp700I GAANNNNTTC 1 cut(s) 2462
AspA2I CCTAGG 1 cut(s) 1903
AspLEI GCGC 2 cut(s) 960, 1743
AspS9I GGNCC 4 cut(s) 25, 819, 1453, 1900
AsuC2I CCSGG 3 cut(s) 1004, 1457, 2265
AvaII GGWCC 4 cut(s) 25, 819, 1453, 1900
AvrII CCTAGG 1 cut(s) 1903
AxyI CCTNAGG 1 cut(s) 1932
BaeGI GKGCMC 1 cut(s) 1004
BamHI GGATCC 1 cut(s) 1082
BanII GRGCYC 1 cut(s) 397
BauI CACGAG 1 cut(s) 1313
BbrPI CACGTG 1 cut(s) 46
BbsI GAAGAC 2 cut(s) 119, 1266
Bbv12I GWGCWC 1 cut(s) 45
BbvI GCAGC 5 cut(s) 200, 842, 864, 1414, 1854
BccI CCATC 5 cut(s) 266, 1256, 1412, 1798, 1877
BciT130I CCWGG 4 cut(s) 68, 1218, 2341, 2487
BclI TGATCA 2 cut(s) 1279, 2034
BcnI CCSGG 3 cut(s) 1004, 1457, 2265
BcoDI GTCTC 4 cut(s) 164, 1117, 2301, 2366
BfmI CTRYAG 1 cut(s) 1077
BfoI RGCGCY 2 cut(s) 961, 1744
BfrI CTTAAG 2 cut(s) 1736, 1759
BisI GCNGC 8 cut(s) 94, 189, 853, 856, 1403, 1498, 1624, 1843
BlnI CCTAGG 1 cut(s) 1903
BlpI GCTNAGC 2 cut(s) 1744, 2321
BlsI GCNGC 8 cut(s) 95, 190, 854, 857, 1404, 1499, 1625, 1844
BmcAI AGTACT 3 cut(s) 753, 2049, 2369
Bme1390I CCNGG 7 cut(s) 68, 1004, 1218, 1457, 2265, 2341, 2487
Bme18I GGWCC 4 cut(s) 25, 819, 1453, 1900
BmgT120I GGNCC 4 cut(s) 25, 819, 1453, 1900
BmiI GGNNCC 5 cut(s) 289, 396, 1084, 2345, 2484
BmrFI CCNGG 7 cut(s) 68, 1004, 1218, 1457, 2265, 2341, 2487
BmsI GCATC 7 cut(s) 325, 409, 690, 867, 1103, 1373, 1757
BpiI GAAGAC 2 cut(s) 119, 1266
BplI GAGNNNNNCTC 2 cut(s) 1296, 1328
BpmI CTGGAG 1 cut(s) 1154
Bpu10I CCTNAGC 1 cut(s) 1598
Bpu1102I GCTNAGC 2 cut(s) 1744, 2321
BpuEI CTTGAG 3 cut(s) 1037, 2039, 2348
BpuMI CCSGG 3 cut(s) 1004, 1457, 2265
BsaAI YACGTR 3 cut(s) 46, 529, 1274
BsaI GGTCTC 1 cut(s) 2301
BsaJI CCNNGG 3 cut(s) 375, 1903, 2486
BsaWI WCCGGW 1 cut(s) 710
Bsc4I CCNNNNNNNGG 3 cut(s) 433, 539, 2431
Bse1I ACTGG 2 cut(s) 458, 2396
Bse21I CCTNAGG 1 cut(s) 1932
Bse3DI GCAATG 2 cut(s) 576, 1235
BseAI TCCGGA 1 cut(s) 710
BseBI CCWGG 4 cut(s) 68, 1218, 2341, 2487
BseDI CCNNGG 3 cut(s) 375, 1903, 2486
BseGI GGATG 6 cut(s) 277, 405, 681, 790, 1564, 1699
BseLI CCNNNNNNNGG 3 cut(s) 433, 539, 2431
BseMI GCAATG 2 cut(s) 576, 1235
BseNI ACTGG 2 cut(s) 458, 2396
BseRI GAGGAG 6 cut(s) 691, 1496, 1499, 1583, 1799, 2187
BseSI GKGCMC 1 cut(s) 1004
BseXI GCAGC 5 cut(s) 200, 842, 864, 1414, 1854
BsgI GTGCAG 2 cut(s) 1421, 1669
BshFI GGCC 7 cut(s) 96, 137, 224, 615, 1184, 1311, 2355
BsiHKAI GWGCWC 1 cut(s) 45
BsiSI CCGG 6 cut(s) 285, 711, 1004, 1456, 2011, 2265
BsiWI CGTACG 1 cut(s) 320
BslFI GGGAC 1 cut(s) 912
BslI CCNNNNNNNGG 3 cut(s) 433, 539, 2431
BsmAI GTCTC 4 cut(s) 164, 1117, 2301, 2366
BsmBI CGTCTC 1 cut(s) 1117
BsmFI GGGAC 1 cut(s) 912
BsmI GAATGC 2 cut(s) 1767, 2004
BsnI GGCC 7 cut(s) 96, 137, 224, 615, 1184, 1311, 2355
Bso31I GGTCTC 1 cut(s) 2301
Bsp1286I GDGCHC 3 cut(s) 45, 397, 1004
Bsp13I TCCGGA 1 cut(s) 710
Bsp1720I GCTNAGC 2 cut(s) 1744, 2321
BspACI CCGC 6 cut(s) 93, 1214, 1367, 1497, 1624, 1908
BspANI GGCC 7 cut(s) 96, 137, 224, 615, 1184, 1311, 2355
BspEI TCCGGA 1 cut(s) 710
BspHI TCATGA 2 cut(s) 595, 1444
BspLI GGNNCC 5 cut(s) 289, 396, 1084, 2345, 2484
BspPI GGATC 7 cut(s) 283, 296, 1077, 1090, 1650, 1719, 1733
BspTI CTTAAG 2 cut(s) 1736, 1759
BspTNI GGTCTC 1 cut(s) 2301
BsrDI GCAATG 2 cut(s) 576, 1235
BsrI ACTGG 2 cut(s) 458, 2396
BssECI CCNNGG 3 cut(s) 375, 1903, 2486
BssSI CACGAG 1 cut(s) 1313
BssT1I CCWWGG 2 cut(s) 375, 1903
Bst2BI CACGAG 1 cut(s) 1313
Bst2UI CCWGG 4 cut(s) 68, 1218, 2341, 2487
Bst4CI ACNGT 3 cut(s) 926, 1690, 2077
Bst6I CTCTTC 1 cut(s) 1721
BstAFI CTTAAG 2 cut(s) 1736, 1759
BstAPI GCANNNNNTGC 1 cut(s) 1234
BstBAI YACGTR 3 cut(s) 46, 529, 1274
BstC8I GCNNGC 2 cut(s) 956, 1235
BstF5I GGATG 6 cut(s) 277, 405, 681, 790, 1564, 1699
BstH2I RGCGCY 2 cut(s) 961, 1744
BstHHI GCGC 2 cut(s) 960, 1743
BstMAI GTCTC 4 cut(s) 164, 1117, 2301, 2366
BstMWI GCNNNNNNNGC 9 cut(s) 77, 143, 713, 1234, 1728, 1999, 2119, 2141, 2185
BstNI CCWGG 4 cut(s) 68, 1218, 2341, 2487
BstNSI RCATGY 1 cut(s) 2063
BstSCI CCNGG 7 cut(s) 66, 1002, 1216, 1455, 2263, 2339, 2485
BstSFI CTRYAG 1 cut(s) 1077
BstSLI GKGCMC 1 cut(s) 1004
BstV1I GCAGC 5 cut(s) 200, 842, 864, 1414, 1854
BstV2I GAAGAC 2 cut(s) 119, 1266
BstX2I RGATCY 3 cut(s) 275, 1082, 1711
BstYI RGATCY 3 cut(s) 275, 1082, 1711
Bsu36I CCTNAGG 1 cut(s) 1932
BsuRI GGCC 7 cut(s) 96, 137, 224, 615, 1184, 1311, 2355
BtgZI GCGATG 1 cut(s) 501
BtsCI GGATG 6 cut(s) 277, 405, 681, 790, 1564, 1699
BtsI GCAGTG 1 cut(s) 2332
BtsIMutI CAGTG 5 cut(s) 237, 664, 1124, 1686, 2332
Cac8I GCNNGC 2 cut(s) 956, 1235
CaiI CAGNNNCTG 1 cut(s) 1777
CciI TCATGA 2 cut(s) 595, 1444
CfoI GCGC 2 cut(s) 960, 1743
Cfr13I GGNCC 4 cut(s) 25, 819, 1453, 1900
CseI GACGC 1 cut(s) 2150
Csp6I GTAC 8 cut(s) 233, 321, 530, 752, 2048, 2070, 2368, 2420
CspCI CAANNNNNGTGG 2 cut(s) 2062, 2097
CviAII CATG 8 cut(s) 596, 781, 1145, 1445, 2004, 2060, 2431, 2469
CviQI GTAC 8 cut(s) 233, 321, 530, 752, 2048, 2070, 2368, 2420
EaeI YGGCCR 1 cut(s) 94
Eam1104I CTCTTC 1 cut(s) 1721
EarI CTCTTC 1 cut(s) 1721
Eco130I CCWWGG 2 cut(s) 375, 1903
Eco147I AGGCCT 3 cut(s) 137, 1311, 2355
Eco24I GRGCYC 1 cut(s) 397
Eco31I GGTCTC 1 cut(s) 2301
Eco32I GATATC 1 cut(s) 343
Eco47I GGWCC 4 cut(s) 25, 819, 1453, 1900
Eco47III AGCGCT 1 cut(s) 1742
Eco57I CTGAAG 5 cut(s) 73, 1244, 1278, 1702, 2477
Eco72I CACGTG 1 cut(s) 46
Eco81I CCTNAGG 1 cut(s) 1932
EcoRI GAATTC 2 cut(s) 741, 1645
EcoRII CCWGG 4 cut(s) 66, 1216, 2339, 2485
EcoRV GATATC 1 cut(s) 343
EcoT14I CCWWGG 2 cut(s) 375, 1903
EcoT38I GRGCYC 1 cut(s) 397
ErhI CCWWGG 2 cut(s) 375, 1903
Esp3I CGTCTC 1 cut(s) 1117
FaeI CATG 8 cut(s) 599, 784, 1148, 1448, 2007, 2063, 2434, 2472
FalI AAGNNNNNCTT 2 cut(s) 1700, 1732
FaqI GGGAC 1 cut(s) 912
FatI CATG 8 cut(s) 595, 780, 1144, 1444, 2003, 2059, 2430, 2468
FauI CCCGC 2 cut(s) 1221, 1901
FbaI TGATCA 2 cut(s) 1279, 2034
FblI GTMKAC 3 cut(s) 1104, 1428, 2388
Fnu4HI GCNGC 8 cut(s) 94, 189, 853, 856, 1403, 1498, 1624, 1843
FokI GGATG 6 cut(s) 284, 392, 668, 797, 1571, 1706
FriOI GRGCYC 1 cut(s) 397
Fsp4HI GCNGC 8 cut(s) 94, 189, 853, 856, 1403, 1498, 1624, 1843
GlaI GCGC 2 cut(s) 959, 1742
GluI GCNGC 8 cut(s) 94, 189, 853, 856, 1403, 1498, 1624, 1843
GsuI CTGGAG 1 cut(s) 1154
HaeII RGCGCY 2 cut(s) 961, 1744
HaeIII GGCC 7 cut(s) 96, 137, 224, 615, 1184, 1311, 2355
HapII CCGG 6 cut(s) 285, 711, 1004, 1456, 2011, 2265
HgaI GACGC 1 cut(s) 2150
HhaI GCGC 2 cut(s) 960, 1743
Hin1II CATG 8 cut(s) 599, 784, 1148, 1448, 2007, 2063, 2434, 2472
Hin6I GCGC 2 cut(s) 958, 1741
HinP1I GCGC 2 cut(s) 958, 1741
HincII GTYRAC 1 cut(s) 1105
HindII GTYRAC 1 cut(s) 1105
HpaII CCGG 6 cut(s) 285, 711, 1004, 1456, 2011, 2265
Hpy166II GTNNAC 6 cut(s) 51, 819, 1055, 1105, 1429, 2389
Hpy188I TCNGA 7 cut(s) 254, 669, 729, 774, 1224, 1664, 1779
Hpy8I GTNNAC 6 cut(s) 51, 819, 1055, 1105, 1429, 2389
Hpy99I CGWCG 2 cut(s) 230, 2144
HpyCH4III ACNGT 3 cut(s) 926, 1690, 2077
HpyCH4IV ACGT 4 cut(s) 45, 528, 1273, 1585
HpyF10VI GCNNNNNNNGC 9 cut(s) 77, 143, 713, 1234, 1728, 1999, 2119, 2141, 2185
HpySE526I ACGT 4 cut(s) 45, 528, 1273, 1585
Hsp92II CATG 8 cut(s) 599, 784, 1148, 1448, 2007, 2063, 2434, 2472
HspAI GCGC 2 cut(s) 958, 1741
Kpn2I TCCGGA 1 cut(s) 710
Ksp22I TGATCA 2 cut(s) 1279, 2034
LmnI GCTCC 6 cut(s) 40, 293, 400, 678, 713, 1736
Lsp1109I GCAGC 5 cut(s) 200, 842, 864, 1414, 1854
LweI GCATC 7 cut(s) 325, 409, 690, 867, 1103, 1373, 1757
MaeII ACGT 4 cut(s) 45, 528, 1273, 1585
MaeIII GTNAC 3 cut(s) 721, 865, 2447
MflI RGATCY 3 cut(s) 275, 1082, 1711
MhlI GDGCHC 3 cut(s) 45, 397, 1004
MlyI GAGTC 7 cut(s) 25, 166, 928, 1050, 1100, 1434, 1996
MmeI TCCRAC 4 cut(s) 459, 485, 1650, 1843
MroI TCCGGA 1 cut(s) 710
MroXI GAANNNNTTC 1 cut(s) 2462
MseI TTAA 9 cut(s) 8, 501, 756, 939, 1286, 1737, 1760, 2259, 2506
MspA1I CMGCKG 3 cut(s) 93, 855, 1722
MspCI CTTAAG 2 cut(s) 1736, 1759
MspI CCGG 6 cut(s) 285, 711, 1004, 1456, 2011, 2265
MspR9I CCNGG 7 cut(s) 68, 1004, 1218, 1457, 2265, 2341, 2487
Mva1269I GAATGC 2 cut(s) 1767, 2004
MvaI CCWGG 4 cut(s) 68, 1218, 2341, 2487
MwoI GCNNNNNNNGC 9 cut(s) 77, 143, 713, 1234, 1728, 1999, 2119, 2141, 2185
NciI CCSGG 3 cut(s) 1004, 1457, 2265
NlaIII CATG 8 cut(s) 599, 784, 1148, 1448, 2007, 2063, 2434, 2472
NlaIV GGNNCC 5 cut(s) 289, 396, 1084, 2345, 2484
NspI RCATGY 1 cut(s) 2063
PagI TCATGA 2 cut(s) 595, 1444
PceI AGGCCT 3 cut(s) 137, 1311, 2355
PciI ACATGT 1 cut(s) 2059
PcsI WCGNNNNNNNCGW 1 cut(s) 922
PctI GAATGC 2 cut(s) 1767, 2004
PdmI GAANNNNTTC 1 cut(s) 2462
PfeI GAWTC 5 cut(s) 457, 796, 918, 1441, 1974
Pfl23II CGTACG 1 cut(s) 320
PflFI GACNNNGTC 1 cut(s) 2312
PflMI CCANNNNNTGG 1 cut(s) 2431
PfoI TCCNGGA 2 cut(s) 1455, 2263
PkrI GCNGC 8 cut(s) 95, 190, 854, 857, 1404, 1499, 1625, 1844
PleI GAGTC 7 cut(s) 25, 165, 928, 1050, 1100, 1433, 1995
PmaCI CACGTG 1 cut(s) 46
PmlI CACGTG 1 cut(s) 46
PpsI GAGTC 7 cut(s) 25, 165, 928, 1050, 1100, 1433, 1995
Ppu21I YACGTR 3 cut(s) 46, 529, 1274
PscI ACATGT 1 cut(s) 2059
PshBI ATTAAT 1 cut(s) 8
Psp6I CCWGG 4 cut(s) 66, 1216, 2339, 2485
PspCI CACGTG 1 cut(s) 46
PspGI CCWGG 4 cut(s) 66, 1216, 2339, 2485
PspLI CGTACG 1 cut(s) 320
PspN4I GGNNCC 5 cut(s) 289, 396, 1084, 2345, 2484
PspPI GGNCC 4 cut(s) 25, 819, 1453, 1900
PstNI CAGNNNCTG 1 cut(s) 1777
PsuI RGATCY 3 cut(s) 275, 1082, 1711
PsyI GACNNNGTC 1 cut(s) 2312
PvuII CAGCTG 2 cut(s) 855, 1722
RsaI GTAC 8 cut(s) 234, 322, 531, 753, 2049, 2071, 2369, 2421
RsaNI GTAC 8 cut(s) 233, 321, 530, 752, 2048, 2070, 2368, 2420
SalI GTCGAC 1 cut(s) 1103
SaqAI TTAA 9 cut(s) 8, 501, 756, 939, 1286, 1737, 1760, 2259, 2506
SatI GCNGC 8 cut(s) 94, 189, 853, 856, 1403, 1498, 1624, 1843
Sau96I GGNCC 4 cut(s) 25, 819, 1453, 1900
ScaI AGTACT 3 cut(s) 753, 2049, 2369
SchI GAGTC 7 cut(s) 25, 166, 928, 1050, 1100, 1434, 1996
ScrFI CCNGG 7 cut(s) 68, 1004, 1218, 1457, 2265, 2341, 2487
SduI GDGCHC 3 cut(s) 45, 397, 1004
SfaNI GCATC 7 cut(s) 325, 409, 690, 867, 1103, 1373, 1757
SfcI CTRYAG 1 cut(s) 1077
SinI GGWCC 4 cut(s) 25, 819, 1453, 1900
SmlI CTYRAG 5 cut(s) 1016, 1736, 1759, 2018, 2363
SmoI CTYRAG 5 cut(s) 1016, 1736, 1759, 2018, 2363
SseBI AGGCCT 3 cut(s) 137, 1311, 2355
SsiI CCGC 6 cut(s) 93, 1214, 1367, 1497, 1624, 1908
StuI AGGCCT 3 cut(s) 137, 1311, 2355
StyD4I CCNGG 7 cut(s) 66, 1002, 1216, 1455, 2263, 2339, 2485
StyI CCWWGG 2 cut(s) 375, 1903
TaaI ACNGT 3 cut(s) 926, 1690, 2077
TaiI ACGT 4 cut(s) 48, 531, 1276, 1588
TaqI TCGA 3 cut(s) 516, 1104, 2139
TatI WGTACW 5 cut(s) 232, 751, 2047, 2367, 2419
TauI GCSGC 3 cut(s) 96, 1500, 1626
TfiI GAWTC 5 cut(s) 457, 796, 918, 1441, 1974
Tru1I TTAA 9 cut(s) 8, 501, 756, 939, 1286, 1737, 1760, 2259, 2506
Tru9I TTAA 9 cut(s) 8, 501, 756, 939, 1286, 1737, 1760, 2259, 2506
TscAI CASTG 5 cut(s) 244, 664, 1124, 1693, 2332
TseI GCWGC 5 cut(s) 188, 852, 855, 1402, 1842
TspGWI ACGGA 2 cut(s) 53, 167
TspRI CASTG 5 cut(s) 244, 664, 1124, 1693, 2332
Tth111I GACNNNGTC 1 cut(s) 2312
Van91I CCANNNNNTGG 1 cut(s) 2431
Vha464I CTTAAG 2 cut(s) 1736, 1759
VpaK11BI GGWCC 4 cut(s) 25, 819, 1453, 1900
VspI ATTAAT 1 cut(s) 8
XapI RAATTY 3 cut(s) 74, 741, 1645
XbaI TCTAGA 2 cut(s) 601, 1849
XceI RCATGY 1 cut(s) 2063
XcmI CCANNNNNNNNNTGG 1 cut(s) 1298
XmaJI CCTAGG 1 cut(s) 1903
XmiI GTMKAC 3 cut(s) 1104, 1428, 2388
XmnI GAANNNNTTC 1 cut(s) 2462
ZrmI AGTACT 3 cut(s) 753, 2049, 2369
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.