Rh4BG032000

NB-ARC domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0000540
Physical Location & Seq
Forward (+)
5729601 .. 5733458
3858 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG032000.1

Sequence Viewer

Length: 2511 bp
ATGTCGTTAGTAGTGGAGGCTATGGTCAAGACTCTAACGGAGCATGTGTTCACAGCTCTAATGAACCAGGCTGAATTTGCACTTGACTTCAGCGGCCAGTTTGAGCAGTTGAAGACAGGGCTTGATCTCACCAAGGCCTTACTTGCAGACACGGAGAGTCTCAACCACAAGAACAACATTGTCAAGGCAGCATTATTTGAGCTGAGAGAAGTCATCTACAAAGCCGACGATGTACTCACTGATTGCTTGGTTAGAGACGAATACAGAAAGGATGGGTCTTGTTCTGGCTCCTTGTTTCACGATTCATTCTTCCTGCATCGTACGGGAAAGAAATTGAAAGATATCAATTTGCGTATGAAGGAGATAGAGCAGACCTTGGGCAAGTTTTTGAGGGCTCCTGATAACATCCATAGAGAAGATGCATACCAAGTTAGGGTGATGGTTTCACAGGACTGGAATCCAACTGAGATAATTGGGTTGGATAGCGATGTGGAGAAGATTAAGGGTTGGCTTTTCGACACTAGCAAGGTACTTTCCCACGTAGGCATTGTGGGAATGGGAGGCTTGGGGAAAACCACCATTGCTCAAAAGATATTTCATGATCTAGAAGTAGTAGGCCACTTTCACAAGCTGATATGGGTGTGTGTTTCGCAATCATTCAGTGCTGATCGGATTATGAGGAGCATCCTAGAAGGTCTAGGAGAAAATGCCTCCGGAGCTGGTGTTACTCAGATTTTGAGCAGAATTCAGCAAGTACTTAAAGATAAGAGCTGTCTGATTGTCATGGATGATGTGTGGAATCAAAAAGATGTTGATTGGTGGACCAACCTCTACTCTGTTATACCCAAACAAAGCAGCTGCATCATCATTACAACTAGAAATGAAGAAGCTGTAATTGGTATGGGAGTTGAAAGCTCACGAATCCACCGTCCCAAGACTCTTAATGATGAGGAAAGCTGGCGCTTGTTCTCCAAGTTTGCATTTCCTGCAAGCAAGGGATTGTGCCCGGAGGAAAGGCTTGAGAAATTAGGAAAAGAACTCTTGAAGAAGTGTGGTGGACTCCCACTCGCAATCAAGACTATAGGATCCTTGTTGGCATCAAAGGTCGACTCCCCTTCTCAGTGGAGACGAGTTCTGGAGAGCTTTCATGCTTTGACAACAGAGGGAAAAACTAGCTCAGTTATGGCCTCTTTGCGATTGAGCTATGATGAACTCCCGCCTGGTCTGAAGCAATGCCTGCTGTGTTTCTCCATCTATCCCGAAGACTATGAGATACGTGCTGATCAGTTAATCCATTGGTGGATTGGAGAAGGCCTCGTGCAAAGCAAGGGCTCAAGAACAGCAGTAGAAGTGGGATATGAGTATCTTGCGGAACTGATAAGCAGATGCTTGGTTGAAATTGTGCAGCAGAGAGGTTTTGATGGGAGAGTCTACAAGTGCAAGATTCATGATATGGTCCGGGAACTGATAATAATGATTGCAGAGGAGGAGGAATTATGCTGCTTTGATGAACAAGGCAGACAGAAATTGACAGCAAATACTAGGTGGTTATGCTTTATTGATGAAATGGAGGAGAAGTCCATGAAAAGTAGTTCAAAGCTCAGGGCATTGTTGATGATGTCAAGCCGCAGATTTGACTTTGAAAGGAATTCTTTGCGATCCCTCAGAATGTTGGATTTATCAAACTGCACTGTGGATGAGCATTGTGTGAAGGATCTCTTCAGCTGGATCAGCTCCCTTAAGCGCTTAGCATCTTTGAACTTAAGTGGGATTCAGGCTCTGAAAGAACTGCCATCTTCAATTCATAAACTCCTCAACCTCCAACTTTTACTTCTAACAGGTTGCAGCAATCTAGAAAAAATACACCCTTCCATCACTAATTTGAAGAAGCTGATTATTTTGGACCTAGGCGGGTGTCCAATCCAATACTTACCTCAGGGATTGGGAAGGCTTTCTTATCTTCAAGAGTTCTCCGGATTCAAGATTGTGAGTCAGCCTAGAATGCCATGTTTCCGGCTTCTTGAGATTAGGGAACTGATCAACCTGAGAGTACTGCGAATACATGTAAGCAGTACCACAGTTATTGCAGACAATGAACTTGATGTCCTCTCTCAGCTTCGAAGGCTCAATGTTCTGGCTATCGACGCAGAAGATTGTAGAAATAACAATGTTTTGGAAATGCTAGAAAGGCTGACCCCTCCTCCAAGCCTCCAAGAGCTGTATCTTAGGAATTACAAGAAGGAAACTCTCCCTAGCTGGGTTAATCCCGGACAGCTTTCAAGATTGCAGTATCTTTGCATCGAGAATGGAGACCTTGTCAAGCTAAGTAGTGGTCAAACCACCTGGAACCTTGAAGGCCTGTGTCTCAAGTACTTGATGAGGCTAGAGGTAGACTGGAAGGACTTGGAGAAGGATATGCCTGTACTCCACTACATGGAGATCAGTCATTGTTACAACTTGAAGGATTTCCCATGTTCAGTTATGGAACCAGGGGTCTGGAGAAAGAATTAA

Protein Analysis

836

Amino Acids

95.44

Weight (kDa)

6.48

Isoelectric Point (pI)

43.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 9 - 94 2e-12 Rx N-terminal domain
NB-ARC PF00931 162 - 331 1.6e-40 NB-ARC domain
AAA_22 PF13401 183 - 265 2.2e-06 AAA domain
WHD_DRP PF23559 418 - 490 2.2e-20 Disease resistance protein Winged helix domain
LRR_14 PF23598 547 - 768 1.3e-27 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 688 - 777 1.4e-08 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000540)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G50950 AT3G50950
fragaria_vesca FvH4_1g12710 FvH4_1g12710 FvH4_3g00820 FvH4_3g00820 FvH4_3g00820 FvH4_3g00820
malus_domestica MD02G1141100.v1.1 MD10G1333100.v1.1
prunus_persica Prupe.4G009300_v2.0.a1 Prupe.4G009300_v2.0.a1 Prupe.4G009300_v2.0.a1 Prupe.6G150700_v2.0.a1 Prupe.7G160100_v2.0.a1 Prupe.7G160100_v2.0.a1
pyrus_communis pycom02g11110 pycom10g28240 pycom10g28340 pycom10g28360 pycom10g28450
rosa_chinensis RchiOBHm_Chr2g0100711 RchiOBHm_Chr3g0485861 RchiOBHm_Chr4g0391451 RchiOBHm_Chr4g0391591 RchiOBHm_Chr4g0391601 RchiOBHm_Chr4g0391681 RchiOBHm_Chr5g0001141 RchiOBHm_Chr5g0001221
rosa_laevigata RLG00000017001
rosa_multiflora Rmu_co8260007.1_g000001 Rmu_co8260103.1_g000001 Rmu_co8519155.1_g000001 Rmu_sc0000060.1_g000050 Rmu_sc0000255.1_g000036 Rmu_sc0001174.1_g000023 Rmu_sc0001174.1_g000025 Rmu_sc0001659.1_g000009 Rmu_sc0001706.1_g000009 Rmu_sc0001706.1_g000024 Rmu_sc0001706.1_g000035 Rmu_sc0004189.1_g000018 Rmu_sc0004189.1_g000030 Rmu_sc0004862.1_g000002 Rmu_sc0004876.1_g000038 Rmu_sc0023741.1_g000004
rosa_roxburghii Rroxscaffold_1G00075100 Rroxscaffold_1G00075160 Rroxscaffold_1G00075210 Rroxscaffold_5G00337160 Rroxscaffold_5G00386590 Rroxscaffold_6G00396300
rosa_rugosa Rorug03G0336200 Rorug03G0336300 Rorug04G0389400
rosa_samantha Rh2AG140400 Rh2BG144100 Rh2CG145400 Rh2DG144900 Rh3AG271200 Rh4AG038000 Rh4AG038700 Rh4BG032000 Rh4BG032800 Rh4CG041100 Rh4CG041300 Rh4DG035500 Rh4DG035600 Rh5AG008800 Rh5AG009100 Rh5BG010900 Rh5BG011500 Rh5BG011600 Rh5BG012000 Rh5CG009400 Rh5CG010000 Rh5DG009800
rosa_wichuraiana Rw2G010940 Rw4G002950 Rw4G002990 Rw4G003000 Rw5G000880 Rw5G000950 Rw5G000960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 2434
AccI GTMKAC 3 cut(s) 1107, 1431, 2391
AccIII TCCGGA 2 cut(s) 713, 1973
AciI CCGC 5 cut(s) 93, 1217, 1370, 1627, 1911
AclWI GGATC 5 cut(s) 1080, 1093, 1653, 1722, 1736
AcoI YGGCCR 1 cut(s) 94
AcsI RAATTY 3 cut(s) 74, 744, 1648
AcuI CTGAAG 3 cut(s) 73, 1247, 1705
AfaI GTAC 8 cut(s) 234, 322, 531, 756, 2052, 2074, 2372, 2424
AfeI AGCGCT 1 cut(s) 1745
AfiI CCNNNNNNNGG 3 cut(s) 433, 2257, 2434
AflII CTTAAG 2 cut(s) 1739, 1762
AflIII ACRYGT 1 cut(s) 2062
AjnI CCWGG 4 cut(s) 66, 1219, 2342, 2488
AjuI GAANNNNNNNTTGG 4 cut(s) 879, 911, 1815, 1847
AloI GAACNNNNNNTCC 4 cut(s) 32, 64, 2088, 2120
Alw26I GTCTC 5 cut(s) 164, 249, 1120, 2304, 2369
AlwI GGATC 5 cut(s) 1080, 1093, 1653, 1722, 1736
AlwNI CAGNNNCTG 1 cut(s) 1780
Aor13HI TCCGGA 2 cut(s) 713, 1973
Aor51HI AGCGCT 1 cut(s) 1745
AoxI GGCC 6 cut(s) 94, 135, 616, 1185, 1312, 2356
ApeKI GCWGC 6 cut(s) 188, 855, 858, 1405, 1500, 1845
ApoI RAATTY 3 cut(s) 74, 744, 1648
ArsI GACNNNNNNTTYG 2 cut(s) 913, 945
Asp700I GAANNNNTTC 2 cut(s) 1951, 2465
AspA2I CCTAGG 1 cut(s) 1906
AspLEI GCGC 2 cut(s) 963, 1746
AspS9I GGNCC 3 cut(s) 822, 1456, 1903
AsuC2I CCSGG 3 cut(s) 1007, 1460, 2268
AsuHPI GGTGA 2 cut(s) 121, 448
AsuII TTCGAA 1 cut(s) 2119
AvaII GGWCC 3 cut(s) 822, 1456, 1903
AvrII CCTAGG 1 cut(s) 1906
AxyI CCTNAGG 1 cut(s) 1935
BaeGI GKGCMC 1 cut(s) 1007
BamHI GGATCC 1 cut(s) 1085
BanII GRGCYC 2 cut(s) 397, 1334
BauI CACGAG 1 cut(s) 1316
BbsI GAAGAC 2 cut(s) 119, 1269
BbvI GCAGC 6 cut(s) 200, 845, 867, 1417, 1487, 1857
BccI CCATC 6 cut(s) 266, 433, 1259, 1415, 1801, 1880
BciT130I CCWGG 4 cut(s) 68, 1221, 2344, 2490
BclI TGATCA 2 cut(s) 1282, 2037
BcnI CCSGG 3 cut(s) 1007, 1460, 2268
BcoDI GTCTC 5 cut(s) 164, 249, 1120, 2304, 2369
BfmI CTRYAG 1 cut(s) 1080
BfoI RGCGCY 2 cut(s) 964, 1747
BfrI CTTAAG 2 cut(s) 1739, 1762
BisI GCNGC 8 cut(s) 94, 189, 856, 859, 1406, 1501, 1627, 1846
BlnI CCTAGG 1 cut(s) 1906
BlpI GCTNAGC 1 cut(s) 1747
BlsI GCNGC 8 cut(s) 95, 190, 857, 860, 1407, 1502, 1628, 1847
BmcAI AGTACT 3 cut(s) 756, 2052, 2372
Bme1390I CCNGG 7 cut(s) 68, 1007, 1221, 1460, 2268, 2344, 2490
Bme18I GGWCC 3 cut(s) 822, 1456, 1903
BmgT120I GGNCC 3 cut(s) 822, 1456, 1903
BmiI GGNNCC 5 cut(s) 289, 396, 1087, 2348, 2487
BmrFI CCNGG 7 cut(s) 68, 1007, 1221, 1460, 2268, 2344, 2490
BmsI GCATC 8 cut(s) 325, 409, 693, 870, 1106, 1376, 1760, 2307
BpiI GAAGAC 2 cut(s) 119, 1269
BplI GAGNNNNNCTC 2 cut(s) 1299, 1331
BpmI CTGGAG 1 cut(s) 1157
Bpu10I CCTNAGC 1 cut(s) 1601
Bpu1102I GCTNAGC 1 cut(s) 1747
Bpu14I TTCGAA 1 cut(s) 2119
BpuEI CTTGAG 4 cut(s) 1040, 1318, 2042, 2351
BpuMI CCSGG 3 cut(s) 1007, 1460, 2268
BsaAI YACGTR 2 cut(s) 541, 1277
BsaI GGTCTC 1 cut(s) 2304
BsaJI CCNNGG 4 cut(s) 132, 375, 1906, 2489
BsaWI WCCGGW 2 cut(s) 713, 1973
Bsc4I CCNNNNNNNGG 3 cut(s) 433, 2257, 2434
Bse1I ACTGG 3 cut(s) 97, 458, 2399
Bse21I CCTNAGG 1 cut(s) 1935
Bse3DI GCAATG 2 cut(s) 579, 1238
BseAI TCCGGA 2 cut(s) 713, 1973
BseBI CCWGG 4 cut(s) 68, 1221, 2344, 2490
BseDI CCNNGG 4 cut(s) 132, 375, 1906, 2489
BseGI GGATG 5 cut(s) 277, 405, 684, 793, 1702
BseLI CCNNNNNNNGG 3 cut(s) 433, 2257, 2434
BseMI GCAATG 2 cut(s) 579, 1238
BseNI ACTGG 3 cut(s) 97, 458, 2399
BseRI GAGGAG 6 cut(s) 694, 1499, 1502, 1586, 1802, 2190
BseSI GKGCMC 1 cut(s) 1007
BseXI GCAGC 6 cut(s) 200, 845, 867, 1417, 1487, 1857
BseYI CCCAGC 1 cut(s) 2256
BsgI GTGCAG 2 cut(s) 1424, 1672
BshFI GGCC 6 cut(s) 96, 137, 618, 1187, 1314, 2358
BsiSI CCGG 6 cut(s) 714, 1007, 1459, 1974, 2014, 2268
BsiWI CGTACG 1 cut(s) 320
BslFI GGGAC 1 cut(s) 915
BslI CCNNNNNNNGG 3 cut(s) 433, 2257, 2434
BsmAI GTCTC 5 cut(s) 164, 249, 1120, 2304, 2369
BsmBI CGTCTC 2 cut(s) 249, 1120
BsmFI GGGAC 1 cut(s) 915
BsmI GAATGC 1 cut(s) 2007
BsnI GGCC 6 cut(s) 96, 137, 618, 1187, 1314, 2358
Bso31I GGTCTC 1 cut(s) 2304
Bsp119I TTCGAA 1 cut(s) 2119
Bsp1286I GDGCHC 3 cut(s) 397, 1007, 1334
Bsp13I TCCGGA 2 cut(s) 713, 1973
Bsp1720I GCTNAGC 1 cut(s) 1747
BspACI CCGC 5 cut(s) 93, 1217, 1370, 1627, 1911
BspANI GGCC 6 cut(s) 96, 137, 618, 1187, 1314, 2358
BspEI TCCGGA 2 cut(s) 713, 1973
BspHI TCATGA 2 cut(s) 598, 1447
BspLI GGNNCC 5 cut(s) 289, 396, 1087, 2348, 2487
BspPI GGATC 5 cut(s) 1080, 1093, 1653, 1722, 1736
BspT104I TTCGAA 1 cut(s) 2119
BspTI CTTAAG 2 cut(s) 1739, 1762
BspTNI GGTCTC 1 cut(s) 2304
BsrDI GCAATG 2 cut(s) 579, 1238
BsrI ACTGG 3 cut(s) 97, 458, 2399
BssECI CCNNGG 4 cut(s) 132, 375, 1906, 2489
BssSI CACGAG 1 cut(s) 1316
BssT1I CCWWGG 3 cut(s) 132, 375, 1906
Bst2BI CACGAG 1 cut(s) 1316
Bst2UI CCWGG 4 cut(s) 68, 1221, 2344, 2490
Bst4CI ACNGT 3 cut(s) 929, 1693, 2080
Bst6I CTCTTC 1 cut(s) 1724
BstAFI CTTAAG 2 cut(s) 1739, 1762
BstAPI GCANNNNNTGC 2 cut(s) 986, 1237
BstBAI YACGTR 2 cut(s) 541, 1277
BstBI TTCGAA 1 cut(s) 2119
BstC8I GCNNGC 3 cut(s) 959, 991, 1238
BstF5I GGATG 5 cut(s) 277, 405, 684, 793, 1702
BstH2I RGCGCY 2 cut(s) 964, 1747
BstHHI GCGC 2 cut(s) 963, 1746
BstMAI GTCTC 5 cut(s) 164, 249, 1120, 2304, 2369
BstNI CCWGG 4 cut(s) 68, 1221, 2344, 2490
BstNSI RCATGY 2 cut(s) 47, 2066
BstSCI CCNGG 7 cut(s) 66, 1005, 1219, 1458, 2266, 2342, 2488
BstSFI CTRYAG 1 cut(s) 1080
BstSLI GKGCMC 1 cut(s) 1007
BstV1I GCAGC 6 cut(s) 200, 845, 867, 1417, 1487, 1857
BstV2I GAAGAC 2 cut(s) 119, 1269
BstX2I RGATCY 2 cut(s) 1085, 1714
BstXI CCANNNNNNTGG 1 cut(s) 2496
BstYI RGATCY 2 cut(s) 1085, 1714
Bsu36I CCTNAGG 1 cut(s) 1935
BsuRI GGCC 6 cut(s) 96, 137, 618, 1187, 1314, 2358
BtgZI GCGATG 1 cut(s) 501
BtsCI GGATG 5 cut(s) 277, 405, 684, 793, 1702
BtsIMutI CAGTG 4 cut(s) 237, 667, 1127, 1689
Cac8I GCNNGC 3 cut(s) 959, 991, 1238
CaiI CAGNNNCTG 1 cut(s) 1780
CciI TCATGA 2 cut(s) 598, 1447
CfoI GCGC 2 cut(s) 963, 1746
Cfr13I GGNCC 3 cut(s) 822, 1456, 1903
CseI GACGC 1 cut(s) 2153
Csp6I GTAC 8 cut(s) 233, 321, 530, 755, 2051, 2073, 2371, 2423
CspCI CAANNNNNGTGG 2 cut(s) 2065, 2100
CviQI GTAC 8 cut(s) 233, 321, 530, 755, 2051, 2073, 2371, 2423
EaeI YGGCCR 1 cut(s) 94
Eam1104I CTCTTC 1 cut(s) 1724
EarI CTCTTC 1 cut(s) 1724
Eco130I CCWWGG 3 cut(s) 132, 375, 1906
Eco147I AGGCCT 3 cut(s) 137, 1314, 2358
Eco24I GRGCYC 2 cut(s) 397, 1334
Eco31I GGTCTC 1 cut(s) 2304
Eco32I GATATC 1 cut(s) 343
Eco47I GGWCC 3 cut(s) 822, 1456, 1903
Eco47III AGCGCT 1 cut(s) 1745
Eco57I CTGAAG 3 cut(s) 73, 1247, 1705
Eco81I CCTNAGG 1 cut(s) 1935
EcoRI GAATTC 2 cut(s) 744, 1648
EcoRII CCWGG 4 cut(s) 66, 1219, 2342, 2488
EcoRV GATATC 1 cut(s) 343
EcoT14I CCWWGG 3 cut(s) 132, 375, 1906
EcoT22I ATGCAT 1 cut(s) 424
EcoT38I GRGCYC 2 cut(s) 397, 1334
ErhI CCWWGG 3 cut(s) 132, 375, 1906
Esp3I CGTCTC 2 cut(s) 249, 1120
FalI AAGNNNNNCTT 6 cut(s) 1636, 1668, 1703, 1735, 1939, 1971
FaqI GGGAC 1 cut(s) 915
FauI CCCGC 2 cut(s) 1224, 1904
FbaI TGATCA 2 cut(s) 1282, 2037
FblI GTMKAC 3 cut(s) 1107, 1431, 2391
Fnu4HI GCNGC 8 cut(s) 94, 189, 856, 859, 1406, 1501, 1627, 1846
FokI GGATG 5 cut(s) 284, 392, 671, 800, 1709
FriOI GRGCYC 2 cut(s) 397, 1334
Fsp4HI GCNGC 8 cut(s) 94, 189, 856, 859, 1406, 1501, 1627, 1846
GlaI GCGC 2 cut(s) 962, 1745
GluI GCNGC 8 cut(s) 94, 189, 856, 859, 1406, 1501, 1627, 1846
GsaI CCCAGC 1 cut(s) 2260
GsuI CTGGAG 1 cut(s) 1157
HaeII RGCGCY 2 cut(s) 964, 1747
HaeIII GGCC 6 cut(s) 96, 137, 618, 1187, 1314, 2358
HapII CCGG 6 cut(s) 714, 1007, 1459, 1974, 2014, 2268
HgaI GACGC 1 cut(s) 2153
HhaI GCGC 2 cut(s) 963, 1746
Hin6I GCGC 2 cut(s) 961, 1744
HinP1I GCGC 2 cut(s) 961, 1744
HincII GTYRAC 1 cut(s) 1108
HindII GTYRAC 1 cut(s) 1108
HpaII CCGG 6 cut(s) 714, 1007, 1459, 1974, 2014, 2268
HphI GGTGA 2 cut(s) 121, 448
Hpy166II GTNNAC 6 cut(s) 51, 822, 1058, 1108, 1432, 2392
Hpy188I TCNGA 6 cut(s) 672, 732, 777, 1227, 1667, 1782
Hpy8I GTNNAC 6 cut(s) 51, 822, 1058, 1108, 1432, 2392
Hpy99I CGWCG 2 cut(s) 230, 2147
HpyCH4III ACNGT 3 cut(s) 929, 1693, 2080
HpyCH4IV ACGT 2 cut(s) 540, 1276
HpySE526I ACGT 2 cut(s) 540, 1276
HspAI GCGC 2 cut(s) 961, 1744
Kpn2I TCCGGA 2 cut(s) 713, 1973
Ksp22I TGATCA 2 cut(s) 1282, 2037
LmnI GCTCC 6 cut(s) 40, 293, 400, 681, 716, 1739
Lsp1109I GCAGC 6 cut(s) 200, 845, 867, 1417, 1487, 1857
LweI GCATC 8 cut(s) 325, 409, 693, 870, 1106, 1376, 1760, 2307
MaeII ACGT 2 cut(s) 540, 1276
MaeIII GTNAC 2 cut(s) 724, 2450
MflI RGATCY 2 cut(s) 1085, 1714
MhlI GDGCHC 3 cut(s) 397, 1007, 1334
MlyI GAGTC 7 cut(s) 25, 166, 931, 1053, 1103, 1437, 1999
MmeI TCCRAC 4 cut(s) 459, 485, 1653, 1846
Mph1103I ATGCAT 1 cut(s) 424
MroI TCCGGA 2 cut(s) 713, 1973
MroXI GAANNNNTTC 2 cut(s) 1951, 2465
MseI TTAA 8 cut(s) 501, 759, 942, 1289, 1740, 1763, 2262, 2509
MspA1I CMGCKG 3 cut(s) 93, 858, 1725
MspCI CTTAAG 2 cut(s) 1739, 1762
MspI CCGG 6 cut(s) 714, 1007, 1459, 1974, 2014, 2268
MspR9I CCNGG 7 cut(s) 68, 1007, 1221, 1460, 2268, 2344, 2490
Mva1269I GAATGC 1 cut(s) 2007
MvaI CCWGG 4 cut(s) 68, 1221, 2344, 2490
NciI CCSGG 3 cut(s) 1007, 1460, 2268
NlaIV GGNNCC 5 cut(s) 289, 396, 1087, 2348, 2487
NsiI ATGCAT 1 cut(s) 424
NspI RCATGY 2 cut(s) 47, 2066
NspV TTCGAA 1 cut(s) 2119
PagI TCATGA 2 cut(s) 598, 1447
PceI AGGCCT 3 cut(s) 137, 1314, 2358
PciI ACATGT 1 cut(s) 2062
PcsI WCGNNNNNNNCGW 1 cut(s) 925
PctI GAATGC 1 cut(s) 2007
PdmI GAANNNNTTC 2 cut(s) 1951, 2465
PfeI GAWTC 7 cut(s) 302, 457, 799, 921, 1444, 1771, 1977
Pfl23II CGTACG 1 cut(s) 320
PflFI GACNNNGTC 1 cut(s) 2315
PflMI CCANNNNNTGG 1 cut(s) 2434
PfoI TCCNGGA 2 cut(s) 1458, 2266
PkrI GCNGC 8 cut(s) 95, 190, 857, 860, 1407, 1502, 1628, 1847
PleI GAGTC 7 cut(s) 25, 165, 931, 1053, 1103, 1436, 1998
PpsI GAGTC 7 cut(s) 25, 165, 931, 1053, 1103, 1436, 1998
Ppu21I YACGTR 2 cut(s) 541, 1277
PscI ACATGT 1 cut(s) 2062
Psp6I CCWGG 4 cut(s) 66, 1219, 2342, 2488
PspFI CCCAGC 1 cut(s) 2256
PspGI CCWGG 4 cut(s) 66, 1219, 2342, 2488
PspLI CGTACG 1 cut(s) 320
PspN4I GGNNCC 5 cut(s) 289, 396, 1087, 2348, 2487
PspPI GGNCC 3 cut(s) 822, 1456, 1903
PstNI CAGNNNCTG 1 cut(s) 1780
PsuI RGATCY 2 cut(s) 1085, 1714
PsyI GACNNNGTC 1 cut(s) 2315
PvuII CAGCTG 2 cut(s) 858, 1725
RsaI GTAC 8 cut(s) 234, 322, 531, 756, 2052, 2074, 2372, 2424
RsaNI GTAC 8 cut(s) 233, 321, 530, 755, 2051, 2073, 2371, 2423
SalI GTCGAC 1 cut(s) 1106
SaqAI TTAA 8 cut(s) 501, 759, 942, 1289, 1740, 1763, 2262, 2509
SatI GCNGC 8 cut(s) 94, 189, 856, 859, 1406, 1501, 1627, 1846
Sau96I GGNCC 3 cut(s) 822, 1456, 1903
ScaI AGTACT 3 cut(s) 756, 2052, 2372
SchI GAGTC 7 cut(s) 25, 166, 931, 1053, 1103, 1437, 1999
ScrFI CCNGG 7 cut(s) 68, 1007, 1221, 1460, 2268, 2344, 2490
SduI GDGCHC 3 cut(s) 397, 1007, 1334
SfaNI GCATC 8 cut(s) 325, 409, 693, 870, 1106, 1376, 1760, 2307
SfcI CTRYAG 1 cut(s) 1080
SfuI TTCGAA 1 cut(s) 2119
SinI GGWCC 3 cut(s) 822, 1456, 1903
SmlI CTYRAG 6 cut(s) 1019, 1333, 1739, 1762, 2021, 2366
SmoI CTYRAG 6 cut(s) 1019, 1333, 1739, 1762, 2021, 2366
SseBI AGGCCT 3 cut(s) 137, 1314, 2358
SsiI CCGC 5 cut(s) 93, 1217, 1370, 1627, 1911
StuI AGGCCT 3 cut(s) 137, 1314, 2358
StyD4I CCNGG 7 cut(s) 66, 1005, 1219, 1458, 2266, 2342, 2488
StyI CCWWGG 3 cut(s) 132, 375, 1906
TaaI ACNGT 3 cut(s) 929, 1693, 2080
TaiI ACGT 2 cut(s) 543, 1279
TaqI TCGA 5 cut(s) 516, 1107, 2119, 2142, 2301
TatI WGTACW 5 cut(s) 232, 754, 2050, 2370, 2422
TauI GCSGC 2 cut(s) 96, 1629
TfiI GAWTC 7 cut(s) 302, 457, 799, 921, 1444, 1771, 1977
Tru1I TTAA 8 cut(s) 501, 759, 942, 1289, 1740, 1763, 2262, 2509
Tru9I TTAA 8 cut(s) 501, 759, 942, 1289, 1740, 1763, 2262, 2509
TscAI CASTG 4 cut(s) 244, 667, 1127, 1696
TseI GCWGC 6 cut(s) 188, 855, 858, 1405, 1500, 1845
TspGWI ACGGA 2 cut(s) 53, 167
TspRI CASTG 4 cut(s) 244, 667, 1127, 1696
Tth111I GACNNNGTC 1 cut(s) 2315
Van91I CCANNNNNTGG 1 cut(s) 2434
Vha464I CTTAAG 2 cut(s) 1739, 1762
VpaK11BI GGWCC 3 cut(s) 822, 1456, 1903
XapI RAATTY 3 cut(s) 74, 744, 1648
XbaI TCTAGA 2 cut(s) 604, 1852
XceI RCATGY 2 cut(s) 47, 2066
XcmI CCANNNNNNNNNTGG 1 cut(s) 1301
XmaJI CCTAGG 1 cut(s) 1906
XmiI GTMKAC 3 cut(s) 1107, 1431, 2391
XmnI GAANNNNTTC 2 cut(s) 1951, 2465
ZrmI AGTACT 3 cut(s) 756, 2052, 2372
Zsp2I ATGCAT 1 cut(s) 424
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.