RchiOBHm_Chr4g0391591

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0000540
Physical Location & Seq
Forward (+)
6969729 .. 6971538
1810 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ36441

Sequence Viewer

Length: 1764 bp
ATGTCGTTAGTAGTGGAGGCTATGGTCAAGACTCTAACGGAGCATGTGTTCACAGCTCTAATGAACCAGGCTGAATTTGCACTTGACTTCAGCGGCCAGTTTGAGCAGTTGAAGACAGGGCTTGATCTCACCAAGGCCTTACTTGCAGACACGGAGAGTCTCAACCACAAGAACAACATTGTCAAGGCAGCATTATTTGAGCTGAGAGAAGTCATCTACAAAGCCGACGATGTACTCACTGATTGCTTGGTTAGAGACGAATACAGAAAGGATGGATCTTGTTCCGGCTCCTTGTTTCACGATCCATTCTTCCTGCATCGTACGGGAAAGAAATTGAAAGACATCAATTTGCGTATGAAGGAGATAGAGCAGACCTTGGGCAAGTTTTTGAGGGCTCCTGATAACATCCATAGAGAAGATGCATACCAAGTTAGGGTGATGGTTTCACAGGACTGGAATCCAACTGAGATAATTGGGTTGGATAGCGATGTGGAGAAGATTAAGGGTTGGCTTTTCGACACTAGCAAGGTACTTTCCCACGTAGGCATTGTGGGAATGGGAGGCTTGGGGAAAACCACCATTGCTCAAGAGATATTTCATGATCTAGAAGTAATTATGAGGAGCATCCTAGAAGGTCTAGGAGAAAATGCCTCCGGAGCTGGTGTTACTCAGATTTTGAGCAGAATTCAGCAAGTACTTAAGGATAAGAGCTGTCTGATTGTCATGGATGATGTGTGGAATCAAACAGATGTTGATTGGTGGACCAACCTCTGCTCTGTTATACCCAAACAAAGCAGCTGCATCATCCTTACAACTAGAAATGAAGAAGCTGTAATTGGTATGGGAGTTGAAAGCTCACGAATCCACCGTCCCAAGACTCTTAATGATGAGGAAAGCTGGCGCTTGTTCTCCAAGTTTGCATTTCCTACAAGCAAGGGATTGTGCCCGGAGGACAGGCTTGAGAAATTAGGAAAAGAACTCTTGAAGAAGTGTGGTGGACTCCCACTCGCAATCAAGACTATAGGATCCTTGTTGGCATCAAAGGTCGACTCCCCTTCTCAGTGGAGACGAGTTCTGGAGAGCTTTCATGCTTTGACAACAGAGGGAAAAACTAGCTCAGTTATGGCCTCTTTGCGATTGAGCTATGATGAACTCCCGCCTGGTCTGAAGCAATGCCTGCTGTGTTTCTCCATCTATCCTGAAGACTATGAGATACGTGCTGATCAGTTAATCCATTGGTGGATTGGAGAAGGCCTCGTGCAAAGCAAGGGCTCAAGAACAGCAGTAGAAGTGGGATATGAGTATCTTGCGGAACTGATAAGCAGATGCTTGGTTGAAATTGTGAAGCAGAGAGGTTTTGATGGGAGAGTCTACAAGTGCAAGATTCATGATATGGTCCGGGAACTGATAATAATGATTACAGAGGAGGAGGAATTATGCTGCTTTGACAAACAAGGCAGACAGAAATTGACAGCAAATACTAGGTGGCTATGCTTTATTGATGAAATGGAGGAGAAGTCCTTGAAACGTAGTTCAAAGCTCAGGGCATTGTTGATGATGTCAAGCCGCAGATTTGACTTTGAAAGGAATTCTCTGCGATCCCTCAGAATGTTGGATTTATCAAACTGCAATGTGGATGAGCATTATGTGAAGGATCTCTTCAGCTGGATCAGCTCCCTTAAGCGCTTAGCATCTTTGAACTTAAGTGGTATTCAGGCTCTGAAAGAAGTGCCATCTTCAATTCATAAACTCCTCAACCTCTAA

Protein Analysis

587

Amino Acids

66.76

Weight (kDa)

6.28

Isoelectric Point (pI)

44.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 9 - 93 1.3e-12 Rx N-terminal domain
NB-ARC PF00931 162 - 205 1.3e-08 NB-ARC domain
NB-ARC PF00931 205 - 311 6.3e-18 NB-ARC domain
WHD_DRP PF23559 398 - 470 1.4e-20 Disease resistance protein Winged helix domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000540)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G50950 AT3G50950
fragaria_vesca FvH4_1g12710 FvH4_1g12710 FvH4_3g00820 FvH4_3g00820 FvH4_3g00820 FvH4_3g00820
malus_domestica MD02G1141100.v1.1 MD10G1333100.v1.1
prunus_persica Prupe.4G009300_v2.0.a1 Prupe.4G009300_v2.0.a1 Prupe.4G009300_v2.0.a1 Prupe.6G150700_v2.0.a1 Prupe.7G160100_v2.0.a1 Prupe.7G160100_v2.0.a1
pyrus_communis pycom02g11110 pycom10g28240 pycom10g28340 pycom10g28360 pycom10g28450
rosa_chinensis RchiOBHm_Chr2g0100711 RchiOBHm_Chr3g0485861 RchiOBHm_Chr4g0391451 RchiOBHm_Chr4g0391591 RchiOBHm_Chr4g0391601 RchiOBHm_Chr4g0391681 RchiOBHm_Chr5g0001141 RchiOBHm_Chr5g0001221
rosa_laevigata RLG00000017001
rosa_multiflora Rmu_co8260007.1_g000001 Rmu_co8260103.1_g000001 Rmu_co8519155.1_g000001 Rmu_sc0000060.1_g000050 Rmu_sc0000255.1_g000036 Rmu_sc0001174.1_g000023 Rmu_sc0001174.1_g000025 Rmu_sc0001659.1_g000009 Rmu_sc0001706.1_g000009 Rmu_sc0001706.1_g000024 Rmu_sc0001706.1_g000035 Rmu_sc0004189.1_g000018 Rmu_sc0004189.1_g000030 Rmu_sc0004862.1_g000002 Rmu_sc0004876.1_g000038 Rmu_sc0023741.1_g000004
rosa_roxburghii Rroxscaffold_1G00075100 Rroxscaffold_1G00075160 Rroxscaffold_1G00075210 Rroxscaffold_5G00337160 Rroxscaffold_5G00386590 Rroxscaffold_6G00396300
rosa_rugosa Rorug03G0336200 Rorug03G0336300 Rorug04G0389400
rosa_samantha Rh2AG140400 Rh2BG144100 Rh2CG145400 Rh2DG144900 Rh3AG271200 Rh4AG038000 Rh4AG038700 Rh4BG032000 Rh4BG032800 Rh4CG041100 Rh4CG041300 Rh4DG035500 Rh4DG035600 Rh5AG008800 Rh5AG009100 Rh5BG010900 Rh5BG011500 Rh5BG011600 Rh5BG012000 Rh5CG009400 Rh5CG010000 Rh5DG009800
rosa_wichuraiana Rw2G010940 Rw4G002950 Rw4G002990 Rw4G003000 Rw5G000880 Rw5G000950 Rw5G000960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 1047, 1371
AccIII TCCGGA 1 cut(s) 653
AciI CCGC 4 cut(s) 93, 1157, 1310, 1567
AclWI GGATC 7 cut(s) 283, 296, 1020, 1033, 1593, 1662, 1676
AcoI YGGCCR 1 cut(s) 94
AcsI RAATTY 3 cut(s) 74, 684, 1588
AcuI CTGAAG 4 cut(s) 73, 1187, 1221, 1645
AfaI GTAC 4 cut(s) 234, 322, 531, 696
AfeI AGCGCT 1 cut(s) 1685
AfiI CCNNNNNNNGG 1 cut(s) 433
AflII CTTAAG 3 cut(s) 698, 1679, 1702
AjnI CCWGG 2 cut(s) 66, 1159
AjuI GAANNNNNNNTTGG 2 cut(s) 819, 851
AloI GAACNNNNNNTCC 2 cut(s) 32, 64
Alw26I GTCTC 3 cut(s) 164, 249, 1060
AlwI GGATC 7 cut(s) 283, 296, 1020, 1033, 1593, 1662, 1676
AlwNI CAGNNNCTG 1 cut(s) 1720
Aor13HI TCCGGA 1 cut(s) 653
Aor51HI AGCGCT 1 cut(s) 1685
AoxI GGCC 4 cut(s) 94, 135, 1125, 1252
ApeKI GCWGC 4 cut(s) 188, 795, 798, 1440
ApoI RAATTY 3 cut(s) 74, 684, 1588
ArsI GACNNNNNNTTYG 2 cut(s) 853, 885
AspLEI GCGC 2 cut(s) 903, 1686
AspS9I GGNCC 2 cut(s) 762, 1396
AsuC2I CCSGG 2 cut(s) 947, 1400
AsuHPI GGTGA 2 cut(s) 121, 448
AvaII GGWCC 2 cut(s) 762, 1396
BaeGI GKGCMC 1 cut(s) 947
BamHI GGATCC 1 cut(s) 1025
BanII GRGCYC 2 cut(s) 397, 1274
BauI CACGAG 1 cut(s) 1256
BbsI GAAGAC 2 cut(s) 119, 1209
BbvI GCAGC 4 cut(s) 200, 785, 807, 1427
BccI CCATC 5 cut(s) 266, 433, 1199, 1355, 1741
BciT130I CCWGG 2 cut(s) 68, 1161
BclI TGATCA 1 cut(s) 1222
BcnI CCSGG 2 cut(s) 947, 1400
BcoDI GTCTC 3 cut(s) 164, 249, 1060
BfaI CTAG 7 cut(s) 522, 605, 629, 638, 816, 1113, 1482
BfmI CTRYAG 1 cut(s) 1020
BfoI RGCGCY 2 cut(s) 904, 1687
BfrI CTTAAG 3 cut(s) 698, 1679, 1702
BisI GCNGC 6 cut(s) 94, 189, 796, 799, 1441, 1567
BlpI GCTNAGC 1 cut(s) 1687
BlsI GCNGC 6 cut(s) 95, 190, 797, 800, 1442, 1568
BmcAI AGTACT 1 cut(s) 696
Bme1390I CCNGG 4 cut(s) 68, 947, 1161, 1400
Bme18I GGWCC 2 cut(s) 762, 1396
BmgT120I GGNCC 2 cut(s) 762, 1396
BmiI GGNNCC 3 cut(s) 289, 396, 1027
BmrFI CCNGG 4 cut(s) 68, 947, 1161, 1400
BmsI GCATC 7 cut(s) 325, 409, 633, 810, 1046, 1316, 1700
BpiI GAAGAC 2 cut(s) 119, 1209
BplI GAGNNNNNCTC 2 cut(s) 1239, 1271
BpmI CTGGAG 1 cut(s) 1097
Bpu10I CCTNAGC 1 cut(s) 1541
Bpu1102I GCTNAGC 1 cut(s) 1687
BpuEI CTTGAG 3 cut(s) 570, 980, 1258
BpuMI CCSGG 2 cut(s) 947, 1400
BsaAI YACGTR 2 cut(s) 541, 1217
BsaJI CCNNGG 2 cut(s) 132, 375
BsaWI WCCGGW 1 cut(s) 653
BsaXI ACNNNNNCTCC 6 cut(s) 32, 62, 648, 678, 1502, 1532
Bsc4I CCNNNNNNNGG 1 cut(s) 433
Bse1I ACTGG 2 cut(s) 97, 458
Bse3DI GCAATG 3 cut(s) 579, 1178, 1636
BseAI TCCGGA 1 cut(s) 653
BseBI CCWGG 2 cut(s) 68, 1161
BseDI CCNNGG 2 cut(s) 132, 375
BseGI GGATG 6 cut(s) 277, 405, 624, 733, 804, 1642
BseLI CCNNNNNNNGG 1 cut(s) 433
BseMI GCAATG 3 cut(s) 579, 1178, 1636
BseMII CTCAG 7 cut(s) 194, 456, 683, 1073, 1131, 1555, 1618
BseNI ACTGG 2 cut(s) 97, 458
BseRI GAGGAG 5 cut(s) 634, 1439, 1442, 1526, 1742
BseSI GKGCMC 1 cut(s) 947
BseXI GCAGC 4 cut(s) 200, 785, 807, 1427
BshFI GGCC 4 cut(s) 96, 137, 1127, 1254
BsiSI CCGG 4 cut(s) 285, 654, 947, 1399
BsiWI CGTACG 1 cut(s) 320
BslFI GGGAC 1 cut(s) 855
BslI CCNNNNNNNGG 1 cut(s) 433
BsmAI GTCTC 3 cut(s) 164, 249, 1060
BsmBI CGTCTC 2 cut(s) 249, 1060
BsmFI GGGAC 1 cut(s) 855
BsnI GGCC 4 cut(s) 96, 137, 1127, 1254
Bsp1286I GDGCHC 3 cut(s) 397, 947, 1274
Bsp13I TCCGGA 1 cut(s) 653
Bsp143I GATC 9 cut(s) 124, 275, 301, 601, 1025, 1222, 1598, 1654, 1668
Bsp1720I GCTNAGC 1 cut(s) 1687
BspACI CCGC 4 cut(s) 93, 1157, 1310, 1567
BspANI GGCC 4 cut(s) 96, 137, 1127, 1254
BspCNI CTCAG 7 cut(s) 195, 457, 682, 1072, 1130, 1554, 1617
BspEI TCCGGA 1 cut(s) 653
BspHI TCATGA 2 cut(s) 598, 1387
BspLI GGNNCC 3 cut(s) 289, 396, 1027
BspPI GGATC 7 cut(s) 283, 296, 1020, 1033, 1593, 1662, 1676
BspTI CTTAAG 3 cut(s) 698, 1679, 1702
BsrDI GCAATG 3 cut(s) 579, 1178, 1636
BsrI ACTGG 2 cut(s) 97, 458
BssECI CCNNGG 2 cut(s) 132, 375
BssMI GATC 9 cut(s) 124, 275, 301, 601, 1025, 1222, 1598, 1654, 1668
BssSI CACGAG 1 cut(s) 1256
BssT1I CCWWGG 2 cut(s) 132, 375
Bst2BI CACGAG 1 cut(s) 1256
Bst2UI CCWGG 2 cut(s) 68, 1161
Bst4CI ACNGT 1 cut(s) 869
Bst6I CTCTTC 1 cut(s) 1664
BstAFI CTTAAG 3 cut(s) 698, 1679, 1702
BstAPI GCANNNNNTGC 1 cut(s) 1177
BstBAI YACGTR 2 cut(s) 541, 1217
BstC8I GCNNGC 2 cut(s) 899, 1178
BstDEI CTNAG 8 cut(s) 203, 465, 669, 1059, 1117, 1541, 1604, 1687
BstF5I GGATG 6 cut(s) 277, 405, 624, 733, 804, 1642
BstH2I RGCGCY 2 cut(s) 904, 1687
BstHHI GCGC 2 cut(s) 903, 1686
BstKTI GATC 9 cut(s) 127, 278, 304, 604, 1028, 1225, 1601, 1657, 1671
BstMAI GTCTC 3 cut(s) 164, 249, 1060
BstMBI GATC 9 cut(s) 124, 275, 301, 601, 1025, 1222, 1598, 1654, 1668
BstMWI GCNNNNNNNGC 5 cut(s) 77, 143, 656, 1177, 1671
BstNI CCWGG 2 cut(s) 68, 1161
BstNSI RCATGY 1 cut(s) 47
BstSCI CCNGG 4 cut(s) 66, 945, 1159, 1398
BstSFI CTRYAG 1 cut(s) 1020
BstSLI GKGCMC 1 cut(s) 947
BstV1I GCAGC 4 cut(s) 200, 785, 807, 1427
BstV2I GAAGAC 2 cut(s) 119, 1209
BstX2I RGATCY 3 cut(s) 275, 1025, 1654
BstYI RGATCY 3 cut(s) 275, 1025, 1654
BsuRI GGCC 4 cut(s) 96, 137, 1127, 1254
BtgZI GCGATG 1 cut(s) 501
BtsCI GGATG 6 cut(s) 277, 405, 624, 733, 804, 1642
BtsIMutI CAGTG 2 cut(s) 237, 1067
Cac8I GCNNGC 2 cut(s) 899, 1178
CaiI CAGNNNCTG 1 cut(s) 1720
CciI TCATGA 2 cut(s) 598, 1387
CfoI GCGC 2 cut(s) 903, 1686
Cfr13I GGNCC 2 cut(s) 762, 1396
Csp6I GTAC 4 cut(s) 233, 321, 530, 695
CviAII CATG 5 cut(s) 44, 599, 724, 1088, 1388
CviQI GTAC 4 cut(s) 233, 321, 530, 695
DdeI CTNAG 8 cut(s) 203, 465, 669, 1059, 1117, 1541, 1604, 1687
DpnI GATC 9 cut(s) 126, 277, 303, 603, 1027, 1224, 1600, 1656, 1670
DpnII GATC 9 cut(s) 124, 275, 301, 601, 1025, 1222, 1598, 1654, 1668
EaeI YGGCCR 1 cut(s) 94
Eam1104I CTCTTC 1 cut(s) 1664
EarI CTCTTC 1 cut(s) 1664
Eco130I CCWWGG 2 cut(s) 132, 375
Eco147I AGGCCT 2 cut(s) 137, 1254
Eco24I GRGCYC 2 cut(s) 397, 1274
Eco47I GGWCC 2 cut(s) 762, 1396
Eco47III AGCGCT 1 cut(s) 1685
Eco57I CTGAAG 4 cut(s) 73, 1187, 1221, 1645
EcoRI GAATTC 2 cut(s) 684, 1588
EcoRII CCWGG 2 cut(s) 66, 1159
EcoT14I CCWWGG 2 cut(s) 132, 375
EcoT22I ATGCAT 1 cut(s) 424
EcoT38I GRGCYC 2 cut(s) 397, 1274
ErhI CCWWGG 2 cut(s) 132, 375
Esp3I CGTCTC 2 cut(s) 249, 1060
FaeI CATG 5 cut(s) 47, 602, 727, 1091, 1391
FalI AAGNNNNNCTT 2 cut(s) 1643, 1675
FaqI GGGAC 1 cut(s) 855
FatI CATG 5 cut(s) 43, 598, 723, 1087, 1387
FauI CCCGC 1 cut(s) 1164
FbaI TGATCA 1 cut(s) 1222
FblI GTMKAC 2 cut(s) 1047, 1371
Fnu4HI GCNGC 6 cut(s) 94, 189, 796, 799, 1441, 1567
FokI GGATG 6 cut(s) 284, 392, 611, 740, 791, 1649
FriOI GRGCYC 2 cut(s) 397, 1274
Fsp4HI GCNGC 6 cut(s) 94, 189, 796, 799, 1441, 1567
FspBI CTAG 7 cut(s) 522, 605, 629, 638, 816, 1113, 1482
GlaI GCGC 2 cut(s) 902, 1685
GluI GCNGC 6 cut(s) 94, 189, 796, 799, 1441, 1567
GsuI CTGGAG 1 cut(s) 1097
HaeII RGCGCY 2 cut(s) 904, 1687
HaeIII GGCC 4 cut(s) 96, 137, 1127, 1254
HapII CCGG 4 cut(s) 285, 654, 947, 1399
HhaI GCGC 2 cut(s) 903, 1686
Hin1II CATG 5 cut(s) 47, 602, 727, 1091, 1391
Hin6I GCGC 2 cut(s) 901, 1684
HinP1I GCGC 2 cut(s) 901, 1684
HincII GTYRAC 1 cut(s) 1048
HindII GTYRAC 1 cut(s) 1048
HpaII CCGG 4 cut(s) 285, 654, 947, 1399
HphI GGTGA 2 cut(s) 121, 448
Hpy166II GTNNAC 5 cut(s) 51, 762, 998, 1048, 1372
Hpy188I TCNGA 5 cut(s) 672, 717, 1167, 1607, 1722
Hpy8I GTNNAC 5 cut(s) 51, 762, 998, 1048, 1372
Hpy99I CGWCG 1 cut(s) 230
HpyAV CCTTC 5 cut(s) 352, 626, 1065, 1244, 1645
HpyCH4III ACNGT 1 cut(s) 869
HpyCH4IV ACGT 3 cut(s) 540, 1216, 1528
HpyCH4V TGCA 9 cut(s) 80, 146, 316, 422, 801, 920, 1261, 1380, 1629
HpyF10VI GCNNNNNNNGC 5 cut(s) 77, 143, 656, 1177, 1671
HpyF3I CTNAG 8 cut(s) 203, 465, 669, 1059, 1117, 1541, 1604, 1687
HpySE526I ACGT 3 cut(s) 540, 1216, 1528
Hsp92II CATG 5 cut(s) 47, 602, 727, 1091, 1391
HspAI GCGC 2 cut(s) 901, 1684
Kpn2I TCCGGA 1 cut(s) 653
Ksp22I TGATCA 1 cut(s) 1222
Kzo9I GATC 9 cut(s) 124, 275, 301, 601, 1025, 1222, 1598, 1654, 1668
LmnI GCTCC 6 cut(s) 40, 293, 400, 621, 656, 1679
Lsp1109I GCAGC 4 cut(s) 200, 785, 807, 1427
LweI GCATC 7 cut(s) 325, 409, 633, 810, 1046, 1316, 1700
MaeI CTAG 7 cut(s) 522, 605, 629, 638, 816, 1113, 1482
MaeII ACGT 3 cut(s) 540, 1216, 1528
MaeIII GTNAC 1 cut(s) 664
MalI GATC 9 cut(s) 126, 277, 303, 603, 1027, 1224, 1600, 1656, 1670
MboI GATC 9 cut(s) 124, 275, 301, 601, 1025, 1222, 1598, 1654, 1668
MboII GAAGA 9 cut(s) 124, 301, 428, 508, 836, 997, 1214, 1651, 1728
MflI RGATCY 3 cut(s) 275, 1025, 1654
MhlI GDGCHC 3 cut(s) 397, 947, 1274
MlyI GAGTC 6 cut(s) 25, 166, 871, 993, 1043, 1377
MmeI TCCRAC 3 cut(s) 459, 485, 1593
Mph1103I ATGCAT 1 cut(s) 424
MroI TCCGGA 1 cut(s) 653
MseI TTAA 6 cut(s) 501, 699, 882, 1229, 1680, 1703
MspA1I CMGCKG 3 cut(s) 93, 798, 1665
MspCI CTTAAG 3 cut(s) 698, 1679, 1702
MspI CCGG 4 cut(s) 285, 654, 947, 1399
MspR9I CCNGG 4 cut(s) 68, 947, 1161, 1400
MvaI CCWGG 2 cut(s) 68, 1161
MwoI GCNNNNNNNGC 5 cut(s) 77, 143, 656, 1177, 1671
NciI CCSGG 2 cut(s) 947, 1400
NdeII GATC 9 cut(s) 124, 275, 301, 601, 1025, 1222, 1598, 1654, 1668
NlaIII CATG 5 cut(s) 47, 602, 727, 1091, 1391
NlaIV GGNNCC 3 cut(s) 289, 396, 1027
NsiI ATGCAT 1 cut(s) 424
NspI RCATGY 1 cut(s) 47
PagI TCATGA 2 cut(s) 598, 1387
PceI AGGCCT 2 cut(s) 137, 1254
PcsI WCGNNNNNNNCGW 1 cut(s) 865
PfeI GAWTC 4 cut(s) 457, 739, 861, 1384
Pfl23II CGTACG 1 cut(s) 320
PfoI TCCNGGA 1 cut(s) 1398
PkrI GCNGC 6 cut(s) 95, 190, 797, 800, 1442, 1568
PleI GAGTC 6 cut(s) 25, 165, 871, 993, 1043, 1376
PpsI GAGTC 6 cut(s) 25, 165, 871, 993, 1043, 1376
Ppu21I YACGTR 2 cut(s) 541, 1217
Psp6I CCWGG 2 cut(s) 66, 1159
PspGI CCWGG 2 cut(s) 66, 1159
PspLI CGTACG 1 cut(s) 320
PspN4I GGNNCC 3 cut(s) 289, 396, 1027
PspPI GGNCC 2 cut(s) 762, 1396
PstNI CAGNNNCTG 1 cut(s) 1720
PsuI RGATCY 3 cut(s) 275, 1025, 1654
PvuII CAGCTG 2 cut(s) 798, 1665
RsaI GTAC 4 cut(s) 234, 322, 531, 696
RsaNI GTAC 4 cut(s) 233, 321, 530, 695
SalI GTCGAC 1 cut(s) 1046
SaqAI TTAA 6 cut(s) 501, 699, 882, 1229, 1680, 1703
SatI GCNGC 6 cut(s) 94, 189, 796, 799, 1441, 1567
Sau3AI GATC 9 cut(s) 124, 275, 301, 601, 1025, 1222, 1598, 1654, 1668
Sau96I GGNCC 2 cut(s) 762, 1396
ScaI AGTACT 1 cut(s) 696
SchI GAGTC 6 cut(s) 25, 166, 871, 993, 1043, 1377
ScrFI CCNGG 4 cut(s) 68, 947, 1161, 1400
SduI GDGCHC 3 cut(s) 397, 947, 1274
SfaNI GCATC 7 cut(s) 325, 409, 633, 810, 1046, 1316, 1700
SfcI CTRYAG 1 cut(s) 1020
SinI GGWCC 2 cut(s) 762, 1396
SmlI CTYRAG 6 cut(s) 585, 698, 959, 1273, 1679, 1702
SmoI CTYRAG 6 cut(s) 585, 698, 959, 1273, 1679, 1702
SseBI AGGCCT 2 cut(s) 137, 1254
SsiI CCGC 4 cut(s) 93, 1157, 1310, 1567
SspMI CTAG 7 cut(s) 522, 605, 629, 638, 816, 1113, 1482
StuI AGGCCT 2 cut(s) 137, 1254
StyD4I CCNGG 4 cut(s) 66, 945, 1159, 1398
StyI CCWWGG 2 cut(s) 132, 375
TaaI ACNGT 1 cut(s) 869
TaiI ACGT 3 cut(s) 543, 1219, 1531
TaqI TCGA 2 cut(s) 516, 1047
TatI WGTACW 2 cut(s) 232, 694
TauI GCSGC 2 cut(s) 96, 1569
TfiI GAWTC 4 cut(s) 457, 739, 861, 1384
Tru1I TTAA 6 cut(s) 501, 699, 882, 1229, 1680, 1703
Tru9I TTAA 6 cut(s) 501, 699, 882, 1229, 1680, 1703
TscAI CASTG 2 cut(s) 244, 1067
TseI GCWGC 4 cut(s) 188, 795, 798, 1440
TspDTI ATGAA 9 cut(s) 77, 371, 587, 837, 1076, 1164, 1376, 1518, 1733
TspGWI ACGGA 2 cut(s) 53, 167
TspRI CASTG 2 cut(s) 244, 1067
Vha464I CTTAAG 3 cut(s) 698, 1679, 1702
VpaK11BI GGWCC 2 cut(s) 762, 1396
XapI RAATTY 3 cut(s) 74, 684, 1588
XbaI TCTAGA 1 cut(s) 604
XceI RCATGY 1 cut(s) 47
XcmI CCANNNNNNNNNTGG 1 cut(s) 1241
XmiI GTMKAC 2 cut(s) 1047, 1371
XspI CTAG 7 cut(s) 522, 605, 629, 638, 816, 1113, 1482
ZrmI AGTACT 1 cut(s) 696
Zsp2I ATGCAT 1 cut(s) 424
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.