RchiOBHm_Chr3g0485861

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0000540
Physical Location & Seq
Reverse (-)
32633949 .. 32636970
3022 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ45043

Sequence Viewer

Length: 2610 bp
ATGGAGACGCTGTTAGGTCCTATGGTTCAAGTTGTACTGGACAAGGTATGTACGGCCATGTTGAACAGAGCTCAAGTCATAGCAAACTTTGAAGACCAGTTTGAGCAGCTGAAGAAAGGGTTAACAACTACGAATGACCTACTTGCCAAAGCGATGGATCTTCAGAGCAAGTATGACGAGGTCATGGGTGCCTTGAGCCAACTAAGAGAAGTAATTGATAAAGCTGATGATGTATTGACAGATTGCCTGGTTCGAGTTACATACAAGGAAAATAAGTCCTCACTACGTTTCTGGTTTCATGATCCGGGATTTAAACTTCGAGTGGGCAAGCAGTTGAAAGATATCAATACGAAAATGGACGTGGCACGTAATACCTTGGGAGAACATTTGAAAATTGCAAACTCTACAGCGCCTGATAGAACCCATAACGGTGAAAGCCATGAAAACCAGAGTGAAAGTACTTGGGAATTCATGTCTCGGAATTACGACCCGGGTGTGACCTATGGATTGGATAATTACATCAAAAGTTTGAAAGAGTGGATGCTTGGCAGTACCAAGGGGAAACTCGACTTTGCTGGCATTGTGGGGATGGGGGGTTTGGGCAAAACCACCATTGCCAAGATGTTCTTTAATGATCCTGAAGTTGTATCTCACTTTGACAAGATGATCTGGGTGCCTGTTTCACGGAACTTTAGTGCGCAGCGGATTCTGAAGTGCATGCTGGAAAAGGCAAATCTGCAAGCACCGGATGTTTTGGAATCTGATAACATGTTCACCAGCCTTAAACGACTTAAAGATCAAGCTTATCTCATCGTCATGGATGATGTGTGGCATAATCCCAATTTATATAGTTTCTGGACTAACCTCCGCGACTCCCTACCAAAAACAGTTAAAAAAAGCAGCTGCATCATAATTACTACTAGGAACATAGAGGTTGCCAATCGTTTGGTGGACCAAGACTCACTAAAATTGCAGCCCGATACTCTGAATGAAAAGGACAGCTGGGCTTTGTTCAGCAGAGTTGCATTTCAATCAACCGAGGTTACCGAGCTGTTCAAGGAGGCAGGACGGGCTATCGTGAATAAATGTGGTGGGCTTCCACTGGCAATCAAGACAGTAGGAGGGTATTTGGCCAAGGAGAGTCTTTTTAAGTGGACCGAGACCATAGAAAAATTCCATTCATTGGCTATCAATAATGAAGAAACAGAATCACTTATGCTTTGTCTACAAGTGAGCTACAATGAACTTCCAACGGAAATGAAGCAGTGCCTATTGTGTCTCTCCGTTTACCCTGAAGACGTTGAGATACATGCTGATCAGCTACTTCACTGGTGGTTTGTAGAGGGCCTCATCCGGAGCAAGAAAGGCAACTCTAAAACAGCACTAGAATTGGGCTATGAATATCTGGCAAAACTAGCCAACAGATGCCTATTGGAAGTTGCGGAAACCCGAGGCTATGATGGAAGAATTTATAAGTTCAAAATACACGATATGATACGGGAATTAATAATCAGAATTGCAGAAAAAGAGAAATTTTGCAACTTCAACGAAGAATGTAGGCAGATACAGATTGAAGAATCTCGGTGGTTATGTATCACTGAAGATATGGCAGCTGAGGTTGAGACGCCGTGGATCAAAAGCAATTCAAGCAATTCAAAGTTGAGGGCACTTTTACTGATGTCAAGCCCTTCAATGATTGACTTCACCAAGAAATTTGTGCTGTTAGAGTCACTCAGGGTATTGGACTTTTCTTACTCCAAGTTAGATGTGATTATTGTTGAAGATAGCAGCACAGCCTCTTCAGGGATTCGTGTAGAGAAACTCTTCGACATGATCACTTCTCTGAAACGCCTAGCATGTCTAAACCTCAGTGGAGTTCAGAAACTATTAGAAGTGCCATCTTCAATTGAGAAACTCCTTAACCTCCAGGTTTTGGTATTGAATGGATGCATAAATTTAGGCAAGATACACGCCAAAATCACAAGTTTGAAGAGGCTTGTTGTCTTGGACATTGGAACATGTCCCCTTGAGCACTTGCCTCGGGACCTAGGGAGACTCTCTGATCTGCAAGAACTCTCTGGATTTAGGGTGGTAAGTCGGGCAAAGACATGGGGTTGCACCCTTCTTCAGCTTGGAAGACTAAAGCAGCTAAGAGTTCTTCGAATTACAATAGGTAATGTGGCTGAAATCTCTGAAGAAGAAAAGAAGAGCCTATCAAGTCTCCAGAACCTCAAGGTTCTAGCTATTGATGCTGCAGATACTAACAAACAAGAAAATCTGGAAATGATAGATGAGCTGGAGTTGCCTCCAAAGATAGAGGAGCTGTATCTCAGGAATTACCGCTCCCGAAAAATGCCAAACTGGTTTAGTCCCTTGAGGCTTCCTAAATTGCATTATCTTTCTATTGAGGATTGCGACGTTGAAAGCCTAAAGGACAACTACAGTTGGAAAATTGAAGGTTTGTATTTAAAATTGTTAGAGATGTTAAAGGTAGACTGGAATGACTTGAAGAGAACCATGCCAGCATTACGCTACATGGAGATTAGCAAATGTTCCAACTTGAGAAACTTTCCAATCGATGTTGTCAAGAAAGCGGTTTGGAGAAAGTGA

Protein Analysis

869

Amino Acids

99.56

Weight (kDa)

7.45

Isoelectric Point (pI)

43.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 8 - 99 8.4e-10 Rx N-terminal domain
NB-ARC PF00931 175 - 346 1.6e-33 NB-ARC domain
WHD_DRP PF23559 429 - 503 3.1e-14 Disease resistance protein Winged helix domain
LRR_14 PF23598 576 - 803 3.7e-23 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 710 - 812 3.4e-11 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000540)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G50950 AT3G50950
fragaria_vesca FvH4_1g12710 FvH4_1g12710 FvH4_3g00820 FvH4_3g00820 FvH4_3g00820 FvH4_3g00820
malus_domestica MD02G1141100.v1.1 MD10G1333100.v1.1
prunus_persica Prupe.4G009300_v2.0.a1 Prupe.4G009300_v2.0.a1 Prupe.4G009300_v2.0.a1 Prupe.6G150700_v2.0.a1 Prupe.7G160100_v2.0.a1 Prupe.7G160100_v2.0.a1
pyrus_communis pycom02g11110 pycom10g28240 pycom10g28340 pycom10g28360 pycom10g28450
rosa_chinensis RchiOBHm_Chr2g0100711 RchiOBHm_Chr3g0485861 RchiOBHm_Chr4g0391451 RchiOBHm_Chr4g0391591 RchiOBHm_Chr4g0391601 RchiOBHm_Chr4g0391681 RchiOBHm_Chr5g0001141 RchiOBHm_Chr5g0001221
rosa_laevigata RLG00000017001
rosa_multiflora Rmu_co8260007.1_g000001 Rmu_co8260103.1_g000001 Rmu_co8519155.1_g000001 Rmu_sc0000060.1_g000050 Rmu_sc0000255.1_g000036 Rmu_sc0001174.1_g000023 Rmu_sc0001174.1_g000025 Rmu_sc0001659.1_g000009 Rmu_sc0001706.1_g000009 Rmu_sc0001706.1_g000024 Rmu_sc0001706.1_g000035 Rmu_sc0004189.1_g000018 Rmu_sc0004189.1_g000030 Rmu_sc0004862.1_g000002 Rmu_sc0004876.1_g000038 Rmu_sc0023741.1_g000004
rosa_roxburghii Rroxscaffold_1G00075100 Rroxscaffold_1G00075160 Rroxscaffold_1G00075210 Rroxscaffold_5G00337160 Rroxscaffold_5G00386590 Rroxscaffold_6G00396300
rosa_rugosa Rorug03G0336200 Rorug03G0336300 Rorug04G0389400
rosa_samantha Rh2AG140400 Rh2BG144100 Rh2CG145400 Rh2DG144900 Rh3AG271200 Rh4AG038000 Rh4AG038700 Rh4BG032000 Rh4BG032800 Rh4CG041100 Rh4CG041300 Rh4DG035500 Rh4DG035600 Rh5AG008800 Rh5AG009100 Rh5BG010900 Rh5BG011500 Rh5BG011600 Rh5BG012000 Rh5CG009400 Rh5CG010000 Rh5DG009800
rosa_wichuraiana Rw2G010940 Rw4G002950 Rw4G002990 Rw4G003000 Rw5G000880 Rw5G000950 Rw5G000960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1473
Acc16I TGCGCA 1 cut(s) 699
AccB1I GGYRCC 2 cut(s) 188, 673
AccB7I CCANNNNNTGG 2 cut(s) 1183, 1933
AccBSI CCGCTC 1 cut(s) 2343
AccI GTMKAC 2 cut(s) 1225, 2493
AccII CGCG 1 cut(s) 870
AccIII TCCGGA 1 cut(s) 1353
AciI CCGC 5 cut(s) 703, 868, 1442, 2341, 2594
AclWI GGATC 4 cut(s) 165, 296, 629, 1640
AcoI YGGCCR 2 cut(s) 54, 1131
AcsI RAATTY 6 cut(s) 467, 1172, 1467, 1532, 1712, 1954
AcuI CTGAAG 9 cut(s) 131, 146, 660, 731, 1314, 1620, 1785, 2111, 2214
AcyI GRCGYC 1 cut(s) 1625
AfaI GTAC 4 cut(s) 36, 52, 460, 553
AfiI CCNNNNNNNGG 3 cut(s) 1183, 1803, 1933
AflIII ACRYGT 2 cut(s) 768, 2018
AjiI CACGTC 1 cut(s) 361
AjnI CCWGG 2 cut(s) 246, 1926
Alw21I GWGCWC 2 cut(s) 73, 2034
Alw26I GTCTC 6 cut(s) 480, 1154, 1283, 1616, 2047, 2225
AlwI GGATC 4 cut(s) 165, 296, 629, 1640
AlwNI CAGNNNCTG 1 cut(s) 413
Ama87I CYCGRG 3 cut(s) 490, 1449, 2040
Aor13HI TCCGGA 1 cut(s) 1353
AoxI GGCC 3 cut(s) 54, 1131, 1345
ApeKI GCWGC 9 cut(s) 106, 700, 900, 903, 973, 1610, 1788, 2146, 2252
ApoI RAATTY 6 cut(s) 467, 1172, 1467, 1532, 1712, 1954
AseI ATTAAT 1 cut(s) 1505
Asp700I GAANNNNTTC 1 cut(s) 1823
AspA2I CCTAGG 1 cut(s) 2047
AspLEI GCGC 2 cut(s) 412, 700
AspS9I GGNCC 5 cut(s) 17, 952, 1155, 1345, 2044
AsuC2I CCSGG 3 cut(s) 306, 491, 492
AsuHPI GGTGA 3 cut(s) 443, 766, 1696
AsuII TTCGAA 1 cut(s) 2161
AvaI CYCGRG 3 cut(s) 490, 1449, 2040
AvaII GGWCC 4 cut(s) 17, 952, 1155, 2044
AvrII CCTAGG 1 cut(s) 2047
BaeGI GKGCMC 1 cut(s) 1669
BalI TGGCCA 1 cut(s) 1133
BanI GGYRCC 2 cut(s) 188, 673
BanII GRGCYC 1 cut(s) 73
BbsI GAAGAC 3 cut(s) 99, 1302, 2143
Bbv12I GWGCWC 2 cut(s) 73, 2034
BbvCI CCTCAGC 1 cut(s) 1614
BbvI GCAGC 9 cut(s) 118, 712, 890, 912, 985, 1622, 1800, 2158, 2239
BccI CCATC 4 cut(s) 148, 583, 1454, 1906
BceAI ACGGC 2 cut(s) 69, 1612
BcgI CGANNNNNNTGC 2 cut(s) 2021, 2055
BciT130I CCWGG 2 cut(s) 248, 1928
BclI TGATCA 2 cut(s) 1315, 1833
BcnI CCSGG 3 cut(s) 306, 491, 492
BcoDI GTCTC 6 cut(s) 480, 1154, 1283, 1616, 2047, 2225
BfaI CTAG 6 cut(s) 921, 1385, 1415, 1853, 2048, 2240
BfmI CTRYAG 3 cut(s) 405, 2253, 2440
BfoI RGCGCY 1 cut(s) 413
BisI GCNGC 9 cut(s) 107, 701, 901, 904, 974, 1611, 1789, 2147, 2253
BlnI CCTAGG 1 cut(s) 2047
BlsI GCNGC 9 cut(s) 108, 702, 902, 905, 975, 1612, 1790, 2148, 2254
BmcAI AGTACT 1 cut(s) 460
Bme1390I CCNGG 5 cut(s) 248, 306, 491, 492, 1928
Bme18I GGWCC 4 cut(s) 17, 952, 1155, 2044
BmeT110I CYCGRG 3 cut(s) 490, 1449, 2040
BmgBI CACGTC 1 cut(s) 361
BmgT120I GGNCC 5 cut(s) 17, 952, 1155, 1345, 2044
BmiI GGNNCC 3 cut(s) 190, 675, 2045
BmrFI CCNGG 5 cut(s) 248, 306, 491, 492, 1928
BmsI GCATC 5 cut(s) 531, 915, 1415, 1937, 2239
BpiI GAAGAC 3 cut(s) 99, 1302, 2143
BpmI CTGGAG 3 cut(s) 1910, 2207, 2318
Bpu10I CCTNAGC 1 cut(s) 1614
Bpu14I TTCGAA 1 cut(s) 2161
BpuEI CTTGAG 6 cut(s) 57, 214, 2048, 2216, 2395, 2581
BpuMI CCSGG 3 cut(s) 306, 491, 492
Bsa29I ATCGAT 1 cut(s) 2577
BsaAI YACGTR 1 cut(s) 368
BsaHI GRCGYC 1 cut(s) 1625
BsaI GGTCTC 1 cut(s) 1154
BsaJI CCNNGG 9 cut(s) 375, 490, 555, 1038, 1134, 1450, 1628, 2039, 2047
BsaWI WCCGGW 2 cut(s) 745, 1353
BsaXI ACNNNNNCTCC 1 cut(s) 26
Bsc4I CCNNNNNNNGG 3 cut(s) 1183, 1803, 1933
Bse1I ACTGG 6 cut(s) 42, 97, 1107, 1334, 2366, 2501
Bse3DI GCAATG 1 cut(s) 612
BseAI TCCGGA 1 cut(s) 1353
BseBI CCWGG 2 cut(s) 248, 1928
BseCI ATCGAT 1 cut(s) 2577
BseDI CCNNGG 9 cut(s) 375, 490, 555, 1038, 1134, 1450, 1628, 2039, 2047
BseGI GGATG 6 cut(s) 546, 594, 754, 826, 1350, 1952
BseLI CCNNNNNNNGG 3 cut(s) 1183, 1803, 1933
BseMI GCAATG 1 cut(s) 612
BseMII CTCAG 4 cut(s) 1605, 1747, 1882, 2344
BseNI ACTGG 6 cut(s) 42, 97, 1107, 1334, 2366, 2501
BseRI GAGGAG 1 cut(s) 2333
BseSI GKGCMC 1 cut(s) 1669
BseXI GCAGC 9 cut(s) 118, 712, 890, 912, 985, 1622, 1800, 2158, 2239
BseYI CCCAGC 1 cut(s) 1002
Bsh1236I CGCG 1 cut(s) 870
BshFI GGCC 3 cut(s) 56, 1133, 1347
BshNI GGYRCC 2 cut(s) 188, 673
BshVI ATCGAT 1 cut(s) 2577
BsiHKAI GWGCWC 2 cut(s) 73, 2034
BsiHKCI CYCGRG 3 cut(s) 490, 1449, 2040
BsiSI CCGG 4 cut(s) 305, 491, 746, 1354
BslFI GGGAC 3 cut(s) 2007, 2057, 2355
BslI CCNNNNNNNGG 3 cut(s) 1183, 1803, 1933
BsmAI GTCTC 6 cut(s) 480, 1154, 1283, 1616, 2047, 2225
BsmBI CGTCTC 1 cut(s) 1616
BsmFI GGGAC 3 cut(s) 2007, 2057, 2355
BsnI GGCC 3 cut(s) 56, 1133, 1347
Bso31I GGTCTC 1 cut(s) 1154
BsoBI CYCGRG 3 cut(s) 490, 1449, 2040
Bsp119I TTCGAA 1 cut(s) 2161
Bsp1286I GDGCHC 3 cut(s) 73, 1669, 2034
Bsp13I TCCGGA 1 cut(s) 1353
Bsp143I GATC 9 cut(s) 157, 301, 634, 666, 796, 1315, 1632, 1833, 2062
BspACI CCGC 5 cut(s) 703, 868, 1442, 2341, 2594
BspANI GGCC 3 cut(s) 56, 1133, 1347
BspCNI CTCAG 4 cut(s) 1606, 1746, 1881, 2343
BspDI ATCGAT 1 cut(s) 2577
BspEI TCCGGA 1 cut(s) 1353
BspFNI CGCG 1 cut(s) 870
BspHI TCATGA 1 cut(s) 298
BspLI GGNNCC 3 cut(s) 190, 675, 2045
BspMAI CTGCAG 1 cut(s) 2257
BspPI GGATC 4 cut(s) 165, 296, 629, 1640
BspQI GCTCTTC 1 cut(s) 2201
BspT104I TTCGAA 1 cut(s) 2161
BspT107I GGYRCC 2 cut(s) 188, 673
BspTNI GGTCTC 1 cut(s) 1154
BsrBI CCGCTC 1 cut(s) 2343
BsrDI GCAATG 1 cut(s) 612
BsrI ACTGG 6 cut(s) 42, 97, 1107, 1334, 2366, 2501
BssECI CCNNGG 9 cut(s) 375, 490, 555, 1038, 1134, 1450, 1628, 2039, 2047
BssMI GATC 9 cut(s) 157, 301, 634, 666, 796, 1315, 1632, 1833, 2062
BssNI GRCGYC 1 cut(s) 1625
BssT1I CCWWGG 4 cut(s) 375, 555, 1134, 2047
Bst2UI CCWGG 2 cut(s) 248, 1928
Bst4CI ACNGT 4 cut(s) 431, 889, 1117, 2444
Bst6I CTCTTC 5 cut(s) 1804, 1829, 1985, 2201, 2504
BstACI GRCGYC 1 cut(s) 1625
BstBAI YACGTR 1 cut(s) 368
BstBI TTCGAA 1 cut(s) 2161
BstC8I GCNNGC 5 cut(s) 329, 577, 719, 741, 2523
BstDEI CTNAG 6 cut(s) 203, 1614, 1733, 1868, 2150, 2330
BstDSI CCRYGG 1 cut(s) 1628
BstEII GGTNACC 1 cut(s) 1042
BstF5I GGATG 6 cut(s) 546, 594, 754, 826, 1350, 1952
BstFNI CGCG 1 cut(s) 870
BstH2I RGCGCY 1 cut(s) 413
BstHHI GCGC 2 cut(s) 412, 700
BstKTI GATC 9 cut(s) 160, 304, 637, 669, 799, 1318, 1635, 1836, 2065
BstMAI GTCTC 6 cut(s) 480, 1154, 1283, 1616, 2047, 2225
BstMBI GATC 9 cut(s) 157, 301, 634, 666, 796, 1315, 1632, 1833, 2062
BstMWI GCNNNNNNNGC 6 cut(s) 1070, 1365, 1415, 1647, 2249, 2302
BstNI CCWGG 2 cut(s) 248, 1928
BstNSI RCATGY 5 cut(s) 721, 772, 1313, 1860, 2022
BstPI GGTNACC 1 cut(s) 1042
BstSCI CCNGG 5 cut(s) 246, 304, 489, 490, 1926
BstSFI CTRYAG 3 cut(s) 405, 2253, 2440
BstSLI GKGCMC 1 cut(s) 1669
BstUI CGCG 1 cut(s) 870
BstV1I GCAGC 9 cut(s) 118, 712, 890, 912, 985, 1622, 1800, 2158, 2239
BstV2I GAAGAC 3 cut(s) 99, 1302, 2143
BstX2I RGATCY 1 cut(s) 157
BstXI CCANNNNNNTGG 2 cut(s) 154, 946
BstYI RGATCY 1 cut(s) 157
Bsu15I ATCGAT 1 cut(s) 2577
BsuRI GGCC 3 cut(s) 56, 1133, 1347
BsuTUI ATCGAT 1 cut(s) 2577
BtgI CCRYGG 1 cut(s) 1628
BtgZI GCGATG 1 cut(s) 167
BtrI CACGTC 1 cut(s) 361
BtsCI GGATG 6 cut(s) 546, 594, 754, 826, 1350, 1952
BtsI GCAGTG 1 cut(s) 1271
BtsIMutI CAGTG 5 cut(s) 1100, 1271, 1327, 1596, 1876
Cac8I GCNNGC 5 cut(s) 329, 577, 719, 741, 2523
CaiI CAGNNNCTG 1 cut(s) 413
CciI TCATGA 1 cut(s) 298
CfoI GCGC 2 cut(s) 412, 700
Cfr13I GGNCC 5 cut(s) 17, 952, 1155, 1345, 2044
Cfr9I CCCGGG 1 cut(s) 490
ClaI ATCGAT 1 cut(s) 2577
CseI GACGC 2 cut(s) 16, 1633
Csp6I GTAC 4 cut(s) 35, 51, 459, 552
CviQI GTAC 4 cut(s) 35, 51, 459, 552
DdeI CTNAG 6 cut(s) 203, 1614, 1733, 1868, 2150, 2330
DpnI GATC 9 cut(s) 159, 303, 636, 668, 798, 1317, 1634, 1835, 2064
DpnII GATC 9 cut(s) 157, 301, 634, 666, 796, 1315, 1632, 1833, 2062
DraI TTTAAA 2 cut(s) 313, 2469
EaeI YGGCCR 2 cut(s) 54, 1131
Eam1104I CTCTTC 5 cut(s) 1804, 1829, 1985, 2201, 2504
EarI CTCTTC 5 cut(s) 1804, 1829, 1985, 2201, 2504
Ecl136II GAGCTC 1 cut(s) 71
Eco130I CCWWGG 4 cut(s) 375, 555, 1134, 2047
Eco24I GRGCYC 1 cut(s) 73
Eco31I GGTCTC 1 cut(s) 1154
Eco32I GATATC 1 cut(s) 343
Eco47I GGWCC 4 cut(s) 17, 952, 1155, 2044
Eco53kI GAGCTC 1 cut(s) 71
Eco57I CTGAAG 9 cut(s) 131, 146, 660, 731, 1314, 1620, 1785, 2111, 2214
Eco88I CYCGRG 3 cut(s) 490, 1449, 2040
Eco91I GGTNACC 1 cut(s) 1042
EcoICRI GAGCTC 1 cut(s) 71
EcoO109I RGGNCCY 3 cut(s) 17, 1345, 2044
EcoO65I GGTNACC 1 cut(s) 1042
EcoRI GAATTC 1 cut(s) 467
EcoRII CCWGG 2 cut(s) 246, 1926
EcoRV GATATC 1 cut(s) 343
EcoT14I CCWWGG 4 cut(s) 375, 555, 1134, 2047
EcoT22I ATGCAT 1 cut(s) 1952
EcoT38I GRGCYC 1 cut(s) 73
ErhI CCWWGG 4 cut(s) 375, 555, 1134, 2047
Esp3I CGTCTC 1 cut(s) 1616
FalI AAGNNNNNCTT 2 cut(s) 611, 643
FaqI GGGAC 3 cut(s) 2007, 2057, 2355
FbaI TGATCA 2 cut(s) 1315, 1833
FblI GTMKAC 2 cut(s) 1225, 2493
Fnu4HI GCNGC 9 cut(s) 107, 701, 901, 904, 974, 1611, 1789, 2147, 2253
FokI GGATG 6 cut(s) 553, 601, 761, 833, 1337, 1959
FriOI GRGCYC 1 cut(s) 73
Fsp4HI GCNGC 9 cut(s) 107, 701, 901, 904, 974, 1611, 1789, 2147, 2253
FspBI CTAG 6 cut(s) 921, 1385, 1415, 1853, 2048, 2240
FspI TGCGCA 1 cut(s) 699
GlaI GCGC 2 cut(s) 411, 699
GluI GCNGC 9 cut(s) 107, 701, 901, 904, 974, 1611, 1789, 2147, 2253
GsaI CCCAGC 1 cut(s) 1006
GsuI CTGGAG 3 cut(s) 1910, 2207, 2318
HaeII RGCGCY 1 cut(s) 413
HaeIII GGCC 3 cut(s) 56, 1133, 1347
HapII CCGG 4 cut(s) 305, 491, 746, 1354
HgaI GACGC 2 cut(s) 16, 1633
HhaI GCGC 2 cut(s) 412, 700
Hin1I GRCGYC 1 cut(s) 1625
Hin6I GCGC 2 cut(s) 410, 698
HinP1I GCGC 2 cut(s) 410, 698
HincII GTYRAC 1 cut(s) 123
HindII GTYRAC 1 cut(s) 123
HindIII AAGCTT 1 cut(s) 801
HpaI GTTAAC 1 cut(s) 123
HpaII CCGG 4 cut(s) 305, 491, 746, 1354
HphI GGTGA 3 cut(s) 443, 766, 1696
Hpy166II GTNNAC 7 cut(s) 123, 774, 952, 1155, 1226, 1288, 2494
Hpy8I GTNNAC 7 cut(s) 123, 774, 952, 1155, 1226, 1288, 2494
Hpy99I CGWCG 1 cut(s) 2420
HpyAV CCTTC 3 cut(s) 1698, 2132, 2450
HpyCH4III ACNGT 4 cut(s) 431, 889, 1117, 2444
HpyCH4IV ACGT 5 cut(s) 286, 360, 367, 1299, 2418
HpyF10VI GCNNNNNNNGC 6 cut(s) 1070, 1365, 1415, 1647, 2249, 2302
HpyF3I CTNAG 6 cut(s) 203, 1614, 1733, 1868, 2150, 2330
HpySE526I ACGT 5 cut(s) 286, 360, 367, 1299, 2418
Hsp92I GRCGYC 1 cut(s) 1625
HspAI GCGC 2 cut(s) 410, 698
Kpn2I TCCGGA 1 cut(s) 1353
Ksp22I TGATCA 2 cut(s) 1315, 1833
KspAI GTTAAC 1 cut(s) 123
Kzo9I GATC 9 cut(s) 157, 301, 634, 666, 796, 1315, 1632, 1833, 2062
LguI GCTCTTC 1 cut(s) 2201
LmnI GCTCC 3 cut(s) 1356, 2320, 2348
Lsp1109I GCAGC 9 cut(s) 118, 712, 890, 912, 985, 1622, 1800, 2158, 2239
LweI GCATC 5 cut(s) 531, 915, 1415, 1937, 2239
MaeI CTAG 6 cut(s) 921, 1385, 1415, 1853, 2048, 2240
MaeII ACGT 5 cut(s) 286, 360, 367, 1299, 2418
MaeIII GTNAC 4 cut(s) 256, 496, 1042, 1728
MalI GATC 9 cut(s) 159, 303, 636, 668, 798, 1317, 1634, 1835, 2064
MbiI CCGCTC 1 cut(s) 2343
MboI GATC 9 cut(s) 157, 301, 634, 666, 796, 1315, 1632, 1833, 2062
MfeI CAATTG 1 cut(s) 1905
MflI RGATCY 1 cut(s) 157
MhlI GDGCHC 3 cut(s) 73, 1669, 2034
MlsI TGGCCA 1 cut(s) 1133
MluNI TGGCCA 1 cut(s) 1133
MlyI GAGTC 5 cut(s) 866, 953, 1150, 1736, 2049
MmeI TCCRAC 3 cut(s) 1274, 2426, 2580
Mox20I TGGCCA 1 cut(s) 1133
Mph1103I ATGCAT 1 cut(s) 1952
MroI TCCGGA 1 cut(s) 1353
MroXI GAANNNNTTC 1 cut(s) 1823
MscI TGGCCA 1 cut(s) 1133
MslI CAYNNNNRTG 2 cut(s) 429, 815
Msp20I TGGCCA 1 cut(s) 1133
MspA1I CMGCKG 5 cut(s) 109, 703, 903, 1002, 1613
MspI CCGG 4 cut(s) 305, 491, 746, 1354
MspR9I CCNGG 5 cut(s) 248, 306, 491, 492, 1928
MunI CAATTG 1 cut(s) 1905
MvaI CCWGG 2 cut(s) 248, 1928
MvnI CGCG 1 cut(s) 870
MwoI GCNNNNNNNGC 6 cut(s) 1070, 1365, 1415, 1647, 2249, 2302
NciI CCSGG 3 cut(s) 306, 491, 492
NdeII GATC 9 cut(s) 157, 301, 634, 666, 796, 1315, 1632, 1833, 2062
NlaIV GGNNCC 3 cut(s) 190, 675, 2045
NmuCI GTSAC 2 cut(s) 496, 1728
NsbI TGCGCA 1 cut(s) 699
NsiI ATGCAT 1 cut(s) 1952
NspI RCATGY 5 cut(s) 721, 772, 1313, 1860, 2022
NspV TTCGAA 1 cut(s) 2161
PaeI GCATGC 1 cut(s) 721
PagI TCATGA 1 cut(s) 298
PciI ACATGT 2 cut(s) 768, 2018
PciSI GCTCTTC 1 cut(s) 2201
PdmI GAANNNNTTC 1 cut(s) 1823
PfeI GAWTC 5 cut(s) 706, 758, 1208, 1577, 1807
PflFI GACNNNGTC 1 cut(s) 179
PflMI CCANNNNNTGG 2 cut(s) 1183, 1933
PfoI TCCNGGA 1 cut(s) 304
PkrI GCNGC 9 cut(s) 108, 702, 902, 905, 975, 1612, 1790, 2148, 2254
PleI GAGTC 5 cut(s) 866, 953, 1149, 1735, 2049
PpsI GAGTC 5 cut(s) 866, 953, 1149, 1735, 2049
Ppu21I YACGTR 1 cut(s) 368
PpuMI RGGWCCY 2 cut(s) 17, 2044
PscI ACATGT 2 cut(s) 768, 2018
PshBI ATTAAT 1 cut(s) 1505
PsiI TTATAA 1 cut(s) 1473
Psp124BI GAGCTC 1 cut(s) 73
Psp5II RGGWCCY 2 cut(s) 17, 2044
Psp6I CCWGG 2 cut(s) 246, 1926
PspEI GGTNACC 1 cut(s) 1042
PspFI CCCAGC 1 cut(s) 1002
PspGI CCWGG 2 cut(s) 246, 1926
PspN4I GGNNCC 3 cut(s) 190, 675, 2045
PspPI GGNCC 5 cut(s) 17, 952, 1155, 1345, 2044
PspPPI RGGWCCY 2 cut(s) 17, 2044
PstI CTGCAG 1 cut(s) 2257
PstNI CAGNNNCTG 1 cut(s) 413
PsuI RGATCY 1 cut(s) 157
PsyI GACNNNGTC 1 cut(s) 179
PvuII CAGCTG 4 cut(s) 109, 903, 1002, 1613
RsaI GTAC 4 cut(s) 36, 52, 460, 553
RsaNI GTAC 4 cut(s) 35, 51, 459, 552
RseI CAYNNNNRTG 2 cut(s) 429, 815
SacI GAGCTC 1 cut(s) 73
SapI GCTCTTC 1 cut(s) 2201
SatI GCNGC 9 cut(s) 107, 701, 901, 904, 974, 1611, 1789, 2147, 2253
Sau3AI GATC 9 cut(s) 157, 301, 634, 666, 796, 1315, 1632, 1833, 2062
Sau96I GGNCC 5 cut(s) 17, 952, 1155, 1345, 2044
ScaI AGTACT 1 cut(s) 460
SchI GAGTC 5 cut(s) 866, 953, 1150, 1736, 2049
ScrFI CCNGG 5 cut(s) 248, 306, 491, 492, 1928
SduI GDGCHC 3 cut(s) 73, 1669, 2034
SfaNI GCATC 5 cut(s) 531, 915, 1415, 1937, 2239
SfcI CTRYAG 3 cut(s) 405, 2253, 2440
SfuI TTCGAA 1 cut(s) 2161
SinI GGWCC 4 cut(s) 17, 952, 1155, 2044
SmaI CCCGGG 1 cut(s) 492
SmiMI CAYNNNNRTG 2 cut(s) 429, 815
SmlI CTYRAG 6 cut(s) 72, 193, 2027, 2231, 2374, 2560
SmoI CTYRAG 6 cut(s) 72, 193, 2027, 2231, 2374, 2560
SphI GCATGC 1 cut(s) 721
SsiI CCGC 5 cut(s) 703, 868, 1442, 2341, 2594
SspMI CTAG 6 cut(s) 921, 1385, 1415, 1853, 2048, 2240
SstI GAGCTC 1 cut(s) 73
StyD4I CCNGG 5 cut(s) 246, 304, 489, 490, 1926
StyI CCWWGG 4 cut(s) 375, 555, 1134, 2047
TaaI ACNGT 4 cut(s) 431, 889, 1117, 2444
TaiI ACGT 5 cut(s) 289, 363, 370, 1302, 2421
TaqI TCGA 6 cut(s) 253, 319, 567, 1827, 2161, 2577
TaqII GACCGA 1 cut(s) 1172
TatI WGTACW 2 cut(s) 34, 458
TfiI GAWTC 5 cut(s) 706, 758, 1208, 1577, 1807
TscAI CASTG 5 cut(s) 1107, 1271, 1334, 1603, 1876
TseFI GTSAC 2 cut(s) 496, 1728
TseI GCWGC 9 cut(s) 106, 700, 900, 903, 973, 1610, 1788, 2146, 2252
Tsp45I GTSAC 2 cut(s) 496, 1728
TspDTI ATGAA 9 cut(s) 287, 456, 460, 1005, 1170, 1212, 1257, 1274, 1413
TspGWI ACGGA 3 cut(s) 700, 1268, 1273
TspMI CCCGGG 1 cut(s) 490
TspRI CASTG 5 cut(s) 1107, 1271, 1334, 1603, 1876
Tth111I GACNNNGTC 1 cut(s) 179
Van91I CCANNNNNTGG 2 cut(s) 1183, 1933
VpaK11BI GGWCC 4 cut(s) 17, 952, 1155, 2044
VspI ATTAAT 1 cut(s) 1505
XapI RAATTY 6 cut(s) 467, 1172, 1467, 1532, 1712, 1954
XceI RCATGY 5 cut(s) 721, 772, 1313, 1860, 2022
XcmI CCANNNNNNNNNTGG 1 cut(s) 946
XmaI CCCGGG 1 cut(s) 490
XmaJI CCTAGG 1 cut(s) 2047
XmiI GTMKAC 2 cut(s) 1225, 2493
XmnI GAANNNNTTC 1 cut(s) 1823
XspI CTAG 6 cut(s) 921, 1385, 1415, 1853, 2048, 2240
ZrmI AGTACT 1 cut(s) 460
Zsp2I ATGCAT 1 cut(s) 1952
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.