Belongs to the disease resistance NB-LRR family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 1476 |
| Acc16I | TGCGCA | 1 cut(s) | 699 |
| AccB1I | GGYRCC | 2 cut(s) | 188, 673 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 1189, 1936 |
| AccBSI | CCGCTC | 1 cut(s) | 2346 |
| AccI | GTMKAC | 2 cut(s) | 1228, 2496 |
| AccII | CGCG | 1 cut(s) | 873 |
| AccIII | TCCGGA | 1 cut(s) | 1356 |
| AciI | CCGC | 5 cut(s) | 703, 871, 1445, 2344, 2597 |
| AclWI | GGATC | 6 cut(s) | 165, 296, 629, 1643, 2623, 2653 |
| AcoI | YGGCCR | 2 cut(s) | 54, 1137 |
| AcsI | RAATTY | 6 cut(s) | 467, 1178, 1470, 1535, 1715, 1957 |
| AcuI | CTGAAG | 7 cut(s) | 131, 146, 731, 1317, 1623, 1788, 2114 |
| AcyI | GRCGYC | 1 cut(s) | 1628 |
| AfaI | GTAC | 4 cut(s) | 36, 52, 460, 553 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 1189, 1806, 1936 |
| AflIII | ACRYGT | 2 cut(s) | 768, 2021 |
| AjnI | CCWGG | 2 cut(s) | 246, 1929 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 611, 643 |
| Alw21I | GWGCWC | 3 cut(s) | 73, 719, 2037 |
| Alw26I | GTCTC | 7 cut(s) | 480, 1160, 1286, 1601, 1619, 2050, 2374 |
| Alw44I | GTGCAC | 1 cut(s) | 715 |
| AlwI | GGATC | 6 cut(s) | 165, 296, 629, 1643, 2623, 2653 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 413 |
| Ama87I | CYCGRG | 3 cut(s) | 490, 1452, 2043 |
| Aor13HI | TCCGGA | 1 cut(s) | 1356 |
| AoxI | GGCC | 3 cut(s) | 54, 1137, 1348 |
| ApaLI | GTGCAC | 1 cut(s) | 715 |
| ApeKI | GCWGC | 9 cut(s) | 106, 700, 903, 906, 976, 1613, 1791, 2149, 2255 |
| ApoI | RAATTY | 6 cut(s) | 467, 1178, 1470, 1535, 1715, 1957 |
| AseI | ATTAAT | 1 cut(s) | 1508 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 626, 1826 |
| AspA2I | CCTAGG | 1 cut(s) | 2050 |
| AspLEI | GCGC | 2 cut(s) | 412, 700 |
| AspS9I | GGNCC | 5 cut(s) | 17, 955, 1161, 1348, 2047 |
| AsuC2I | CCSGG | 3 cut(s) | 306, 491, 492 |
| AsuHPI | GGTGA | 4 cut(s) | 443, 766, 1699, 2630 |
| AsuII | TTCGAA | 1 cut(s) | 2164 |
| AvaI | CYCGRG | 3 cut(s) | 490, 1452, 2043 |
| AvaII | GGWCC | 4 cut(s) | 17, 955, 1161, 2047 |
| AvrII | CCTAGG | 1 cut(s) | 2050 |
| BaeGI | GKGCMC | 2 cut(s) | 719, 1672 |
| BalI | TGGCCA | 1 cut(s) | 1139 |
| BanI | GGYRCC | 2 cut(s) | 188, 673 |
| BanII | GRGCYC | 1 cut(s) | 73 |
| BbsI | GAAGAC | 3 cut(s) | 99, 1305, 2146 |
| Bbv12I | GWGCWC | 3 cut(s) | 73, 719, 2037 |
| BbvCI | CCTCAGC | 2 cut(s) | 1617, 1664 |
| BbvI | GCAGC | 9 cut(s) | 118, 712, 893, 915, 988, 1625, 1803, 2161, 2242 |
| BccI | CCATC | 4 cut(s) | 148, 583, 1457, 1909 |
| BceAI | ACGGC | 2 cut(s) | 69, 1615 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 2024, 2058 |
| BciT130I | CCWGG | 2 cut(s) | 248, 1931 |
| BclI | TGATCA | 2 cut(s) | 1318, 1836 |
| BcnI | CCSGG | 3 cut(s) | 306, 491, 492 |
| BcoDI | GTCTC | 7 cut(s) | 480, 1160, 1286, 1601, 1619, 2050, 2374 |
| BfaI | CTAG | 7 cut(s) | 924, 1325, 1388, 1418, 1856, 2051, 2243 |
| BfmI | CTRYAG | 3 cut(s) | 405, 2256, 2443 |
| BfoI | RGCGCY | 1 cut(s) | 413 |
| BisI | GCNGC | 9 cut(s) | 107, 701, 904, 907, 977, 1614, 1792, 2150, 2256 |
| BlnI | CCTAGG | 1 cut(s) | 2050 |
| BlsI | GCNGC | 9 cut(s) | 108, 702, 905, 908, 978, 1615, 1793, 2151, 2257 |
| BmcAI | AGTACT | 1 cut(s) | 460 |
| Bme1390I | CCNGG | 5 cut(s) | 248, 306, 491, 492, 1931 |
| Bme18I | GGWCC | 4 cut(s) | 17, 955, 1161, 2047 |
| BmeT110I | CYCGRG | 3 cut(s) | 490, 1452, 2043 |
| BmgT120I | GGNCC | 5 cut(s) | 17, 955, 1161, 1348, 2047 |
| BmiI | GGNNCC | 3 cut(s) | 190, 675, 2048 |
| BmrFI | CCNGG | 5 cut(s) | 248, 306, 491, 492, 1931 |
| BmsI | GCATC | 4 cut(s) | 531, 918, 1940, 2242 |
| BpiI | GAAGAC | 3 cut(s) | 99, 1305, 2146 |
| BpmI | CTGGAG | 3 cut(s) | 1913, 2210, 2321 |
| Bpu10I | CCTNAGC | 2 cut(s) | 1617, 1664 |
| Bpu14I | TTCGAA | 1 cut(s) | 2164 |
| BpuEI | CTTGAG | 5 cut(s) | 57, 214, 2051, 2219, 2398 |
| BpuMI | CCSGG | 3 cut(s) | 306, 491, 492 |
| BsaAI | YACGTR | 1 cut(s) | 368 |
| BsaHI | GRCGYC | 1 cut(s) | 1628 |
| BsaI | GGTCTC | 1 cut(s) | 1160 |
| BsaWI | WCCGGW | 2 cut(s) | 745, 1356 |
| BsaXI | ACNNNNNCTCC | 3 cut(s) | 26, 2596, 2626 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 1189, 1806, 1936 |
| Bse1I | ACTGG | 7 cut(s) | 42, 97, 1113, 1337, 2369, 2504, 2576 |
| Bse3DI | GCAATG | 1 cut(s) | 612 |
| BseAI | TCCGGA | 1 cut(s) | 1356 |
| BseBI | CCWGG | 2 cut(s) | 248, 1931 |
| BseGI | GGATG | 7 cut(s) | 546, 594, 754, 829, 1353, 1955, 2587 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 1189, 1806, 1936 |
| BseMI | GCAATG | 1 cut(s) | 612 |
| BseMII | CTCAG | 5 cut(s) | 1608, 1655, 1750, 1885, 2347 |
| BseNI | ACTGG | 7 cut(s) | 42, 97, 1113, 1337, 2369, 2504, 2576 |
| BseRI | GAGGAG | 1 cut(s) | 2336 |
| BseSI | GKGCMC | 2 cut(s) | 719, 1672 |
| BseXI | GCAGC | 9 cut(s) | 118, 712, 893, 915, 988, 1625, 1803, 2161, 2242 |
| BseYI | CCCAGC | 1 cut(s) | 1005 |
| Bsh1236I | CGCG | 1 cut(s) | 873 |
| BshFI | GGCC | 3 cut(s) | 56, 1139, 1350 |
| BshNI | GGYRCC | 2 cut(s) | 188, 673 |
| BsiHKAI | GWGCWC | 3 cut(s) | 73, 719, 2037 |
| BsiHKCI | CYCGRG | 3 cut(s) | 490, 1452, 2043 |
| BsiSI | CCGG | 4 cut(s) | 305, 491, 746, 1357 |
| BslFI | GGGAC | 2 cut(s) | 2010, 2060 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 1189, 1806, 1936 |
| BsmAI | GTCTC | 7 cut(s) | 480, 1160, 1286, 1601, 1619, 2050, 2374 |
| BsmBI | CGTCTC | 1 cut(s) | 1619 |
| BsmFI | GGGAC | 2 cut(s) | 2010, 2060 |
| BsnI | GGCC | 3 cut(s) | 56, 1139, 1350 |
| Bso31I | GGTCTC | 1 cut(s) | 1160 |
| BsoBI | CYCGRG | 3 cut(s) | 490, 1452, 2043 |
| Bsp119I | TTCGAA | 1 cut(s) | 2164 |
| Bsp1286I | GDGCHC | 4 cut(s) | 73, 719, 1672, 2037 |
| Bsp13I | TCCGGA | 1 cut(s) | 1356 |
| Bsp19I | CCATGG | 1 cut(s) | 2641 |
| BspACI | CCGC | 5 cut(s) | 703, 871, 1445, 2344, 2597 |
| BspANI | GGCC | 3 cut(s) | 56, 1139, 1350 |
| BspCNI | CTCAG | 5 cut(s) | 1609, 1656, 1749, 1884, 2346 |
| BspEI | TCCGGA | 1 cut(s) | 1356 |
| BspFNI | CGCG | 1 cut(s) | 873 |
| BspHI | TCATGA | 1 cut(s) | 298 |
| BspLI | GGNNCC | 3 cut(s) | 190, 675, 2048 |
| BspMAI | CTGCAG | 2 cut(s) | 2260, 2447 |
| BspPI | GGATC | 6 cut(s) | 165, 296, 629, 1643, 2623, 2653 |
| BspQI | GCTCTTC | 1 cut(s) | 2204 |
| BspT104I | TTCGAA | 1 cut(s) | 2164 |
| BspT107I | GGYRCC | 2 cut(s) | 188, 673 |
| BspTNI | GGTCTC | 1 cut(s) | 1160 |
| BsrBI | CCGCTC | 1 cut(s) | 2346 |
| BsrDI | GCAATG | 1 cut(s) | 612 |
| BsrI | ACTGG | 7 cut(s) | 42, 97, 1113, 1337, 2369, 2504, 2576 |
| BssNI | GRCGYC | 1 cut(s) | 1628 |
| BssT1I | CCWWGG | 5 cut(s) | 375, 555, 1140, 2050, 2641 |
| Bst2UI | CCWGG | 2 cut(s) | 248, 1931 |
| Bst4CI | ACNGT | 3 cut(s) | 431, 892, 1123 |
| Bst6I | CTCTTC | 6 cut(s) | 990, 1807, 1832, 1988, 2204, 2507 |
| BstACI | GRCGYC | 1 cut(s) | 1628 |
| BstBAI | YACGTR | 1 cut(s) | 368 |
| BstBI | TTCGAA | 1 cut(s) | 2164 |
| BstC8I | GCNNGC | 5 cut(s) | 329, 577, 719, 741, 2526 |
| BstDEI | CTNAG | 9 cut(s) | 203, 1617, 1664, 1736, 1871, 2106, 2153, 2333, 2649 |
| BstDSI | CCRYGG | 2 cut(s) | 1631, 2641 |
| BstEII | GGTNACC | 1 cut(s) | 1045 |
| BstF5I | GGATG | 7 cut(s) | 546, 594, 754, 829, 1353, 1955, 2587 |
| BstFNI | CGCG | 1 cut(s) | 873 |
| BstH2I | RGCGCY | 1 cut(s) | 413 |
| BstHHI | GCGC | 2 cut(s) | 412, 700 |
| BstMAI | GTCTC | 7 cut(s) | 480, 1160, 1286, 1601, 1619, 2050, 2374 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 1076, 1368, 1418, 1650, 2252, 2305 |
| BstNI | CCWGG | 2 cut(s) | 248, 1931 |
| BstNSI | RCATGY | 4 cut(s) | 772, 1316, 1863, 2025 |
| BstPI | GGTNACC | 1 cut(s) | 1045 |
| BstSCI | CCNGG | 5 cut(s) | 246, 304, 489, 490, 1929 |
| BstSFI | CTRYAG | 3 cut(s) | 405, 2256, 2443 |
| BstSLI | GKGCMC | 2 cut(s) | 719, 1672 |
| BstUI | CGCG | 1 cut(s) | 873 |
| BstV1I | GCAGC | 9 cut(s) | 118, 712, 893, 915, 988, 1625, 1803, 2161, 2242 |
| BstV2I | GAAGAC | 3 cut(s) | 99, 1305, 2146 |
| BstX2I | RGATCY | 2 cut(s) | 157, 2645 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 154 |
| BstYI | RGATCY | 2 cut(s) | 157, 2645 |
| BsuRI | GGCC | 3 cut(s) | 56, 1139, 1350 |
| BtgI | CCRYGG | 2 cut(s) | 1631, 2641 |
| BtgZI | GCGATG | 1 cut(s) | 167 |
| BtsCI | GGATG | 7 cut(s) | 546, 594, 754, 829, 1353, 1955, 2587 |
| BtsI | GCAGTG | 1 cut(s) | 1274 |
| BtsIMutI | CAGTG | 6 cut(s) | 1106, 1274, 1330, 1599, 1879, 2583 |
| Cac8I | GCNNGC | 5 cut(s) | 329, 577, 719, 741, 2526 |
| CaiI | CAGNNNCTG | 1 cut(s) | 413 |
| CciI | TCATGA | 1 cut(s) | 298 |
| CfoI | GCGC | 2 cut(s) | 412, 700 |
| Cfr13I | GGNCC | 5 cut(s) | 17, 955, 1161, 1348, 2047 |
| Cfr9I | CCCGGG | 1 cut(s) | 490 |
| CseI | GACGC | 2 cut(s) | 16, 1636 |
| Csp6I | GTAC | 4 cut(s) | 35, 51, 459, 552 |
| CviQI | GTAC | 4 cut(s) | 35, 51, 459, 552 |
| DdeI | CTNAG | 9 cut(s) | 203, 1617, 1664, 1736, 1871, 2106, 2153, 2333, 2649 |
| DraI | TTTAAA | 2 cut(s) | 313, 2472 |
| EaeI | YGGCCR | 2 cut(s) | 54, 1137 |
| Eam1104I | CTCTTC | 6 cut(s) | 990, 1807, 1832, 1988, 2204, 2507 |
| EarI | CTCTTC | 6 cut(s) | 990, 1807, 1832, 1988, 2204, 2507 |
| Ecl136II | GAGCTC | 1 cut(s) | 71 |
| Eco130I | CCWWGG | 5 cut(s) | 375, 555, 1140, 2050, 2641 |
| Eco24I | GRGCYC | 1 cut(s) | 73 |
| Eco31I | GGTCTC | 1 cut(s) | 1160 |
| Eco32I | GATATC | 1 cut(s) | 343 |
| Eco47I | GGWCC | 4 cut(s) | 17, 955, 1161, 2047 |
| Eco53kI | GAGCTC | 1 cut(s) | 71 |
| Eco57I | CTGAAG | 7 cut(s) | 131, 146, 731, 1317, 1623, 1788, 2114 |
| Eco88I | CYCGRG | 3 cut(s) | 490, 1452, 2043 |
| Eco91I | GGTNACC | 1 cut(s) | 1045 |
| EcoICRI | GAGCTC | 1 cut(s) | 71 |
| EcoO109I | RGGNCCY | 3 cut(s) | 17, 1348, 2047 |
| EcoO65I | GGTNACC | 1 cut(s) | 1045 |
| EcoRI | GAATTC | 1 cut(s) | 467 |
| EcoRII | CCWGG | 2 cut(s) | 246, 1929 |
| EcoRV | GATATC | 1 cut(s) | 343 |
| EcoT14I | CCWWGG | 5 cut(s) | 375, 555, 1140, 2050, 2641 |
| EcoT22I | ATGCAT | 1 cut(s) | 1955 |
| EcoT38I | GRGCYC | 1 cut(s) | 73 |
| ErhI | CCWWGG | 5 cut(s) | 375, 555, 1140, 2050, 2641 |
| Esp3I | CGTCTC | 1 cut(s) | 1619 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 611, 643 |
| FaqI | GGGAC | 2 cut(s) | 2010, 2060 |
| FbaI | TGATCA | 2 cut(s) | 1318, 1836 |
| FblI | GTMKAC | 2 cut(s) | 1228, 2496 |
| Fnu4HI | GCNGC | 9 cut(s) | 107, 701, 904, 907, 977, 1614, 1792, 2150, 2256 |
| FokI | GGATG | 7 cut(s) | 553, 601, 761, 836, 1340, 1962, 2594 |
| FriOI | GRGCYC | 1 cut(s) | 73 |
| Fsp4HI | GCNGC | 9 cut(s) | 107, 701, 904, 907, 977, 1614, 1792, 2150, 2256 |
| FspBI | CTAG | 7 cut(s) | 924, 1325, 1388, 1418, 1856, 2051, 2243 |
| FspI | TGCGCA | 1 cut(s) | 699 |
| GlaI | GCGC | 2 cut(s) | 411, 699 |
| GluI | GCNGC | 9 cut(s) | 107, 701, 904, 907, 977, 1614, 1792, 2150, 2256 |
| GsaI | CCCAGC | 1 cut(s) | 1009 |
| GsuI | CTGGAG | 3 cut(s) | 1913, 2210, 2321 |
| HaeII | RGCGCY | 1 cut(s) | 413 |
| HaeIII | GGCC | 3 cut(s) | 56, 1139, 1350 |
| HapII | CCGG | 4 cut(s) | 305, 491, 746, 1357 |
| HgaI | GACGC | 2 cut(s) | 16, 1636 |
| HhaI | GCGC | 2 cut(s) | 412, 700 |
| Hin1I | GRCGYC | 1 cut(s) | 1628 |
| Hin6I | GCGC | 2 cut(s) | 410, 698 |
| HinP1I | GCGC | 2 cut(s) | 410, 698 |
| HincII | GTYRAC | 1 cut(s) | 123 |
| HindII | GTYRAC | 1 cut(s) | 123 |
| HindIII | AAGCTT | 1 cut(s) | 804 |
| HinfI | GANTC | 9 cut(s) | 706, 758, 875, 962, 1147, 1211, 1580, 1730, 2058 |
| HpaI | GTTAAC | 1 cut(s) | 123 |
| HpaII | CCGG | 4 cut(s) | 305, 491, 746, 1357 |
| HphI | GGTGA | 4 cut(s) | 443, 766, 1699, 2630 |
| Hpy166II | GTNNAC | 9 cut(s) | 123, 717, 774, 955, 1161, 1229, 1291, 1330, 2497 |
| Hpy8I | GTNNAC | 9 cut(s) | 123, 717, 774, 955, 1161, 1229, 1291, 1330, 2497 |
| Hpy99I | CGWCG | 1 cut(s) | 2423 |
| HpyAV | CCTTC | 4 cut(s) | 1057, 1701, 2135, 2453 |
| HpyCH4III | ACNGT | 3 cut(s) | 431, 892, 1123 |
| HpyCH4IV | ACGT | 4 cut(s) | 286, 367, 1302, 2421 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 1076, 1368, 1418, 1650, 2252, 2305 |
| HpyF3I | CTNAG | 9 cut(s) | 203, 1617, 1664, 1736, 1871, 2106, 2153, 2333, 2649 |
| HpySE526I | ACGT | 4 cut(s) | 286, 367, 1302, 2421 |
| Hsp92I | GRCGYC | 1 cut(s) | 1628 |
| HspAI | GCGC | 2 cut(s) | 410, 698 |
| Kpn2I | TCCGGA | 1 cut(s) | 1356 |
| Ksp22I | TGATCA | 2 cut(s) | 1318, 1836 |
| KspAI | GTTAAC | 1 cut(s) | 123 |
| LguI | GCTCTTC | 1 cut(s) | 2204 |
| LmnI | GCTCC | 3 cut(s) | 1359, 2323, 2351 |
| Lsp1109I | GCAGC | 9 cut(s) | 118, 712, 893, 915, 988, 1625, 1803, 2161, 2242 |
| LweI | GCATC | 4 cut(s) | 531, 918, 1940, 2242 |
| MaeI | CTAG | 7 cut(s) | 924, 1325, 1388, 1418, 1856, 2051, 2243 |
| MaeII | ACGT | 4 cut(s) | 286, 367, 1302, 2421 |
| MaeIII | GTNAC | 4 cut(s) | 256, 496, 1045, 1731 |
| MbiI | CCGCTC | 1 cut(s) | 2346 |
| MfeI | CAATTG | 1 cut(s) | 1908 |
| MflI | RGATCY | 2 cut(s) | 157, 2645 |
| MhlI | GDGCHC | 4 cut(s) | 73, 719, 1672, 2037 |
| MlsI | TGGCCA | 1 cut(s) | 1139 |
| MluNI | TGGCCA | 1 cut(s) | 1139 |
| MlyI | GAGTC | 5 cut(s) | 869, 956, 1156, 1739, 2052 |
| MmeI | TCCRAC | 4 cut(s) | 1277, 1707, 2429, 2583 |
| Mox20I | TGGCCA | 1 cut(s) | 1139 |
| Mph1103I | ATGCAT | 1 cut(s) | 1955 |
| MroI | TCCGGA | 1 cut(s) | 1356 |
| MroXI | GAANNNNTTC | 2 cut(s) | 626, 1826 |
| MscI | TGGCCA | 1 cut(s) | 1139 |
| MslI | CAYNNNNRTG | 2 cut(s) | 429, 818 |
| Msp20I | TGGCCA | 1 cut(s) | 1139 |
| MspA1I | CMGCKG | 5 cut(s) | 109, 703, 906, 1005, 1616 |
| MspI | CCGG | 4 cut(s) | 305, 491, 746, 1357 |
| MspR9I | CCNGG | 5 cut(s) | 248, 306, 491, 492, 1931 |
| MunI | CAATTG | 1 cut(s) | 1908 |
| MvaI | CCWGG | 2 cut(s) | 248, 1931 |
| MvnI | CGCG | 1 cut(s) | 873 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 1076, 1368, 1418, 1650, 2252, 2305 |
| NciI | CCSGG | 3 cut(s) | 306, 491, 492 |
| NcoI | CCATGG | 1 cut(s) | 2641 |
| NlaIV | GGNNCC | 3 cut(s) | 190, 675, 2048 |
| NmuCI | GTSAC | 2 cut(s) | 496, 1731 |
| NsbI | TGCGCA | 1 cut(s) | 699 |
| NsiI | ATGCAT | 1 cut(s) | 1955 |
| NspI | RCATGY | 4 cut(s) | 772, 1316, 1863, 2025 |
| NspV | TTCGAA | 1 cut(s) | 2164 |
| PagI | TCATGA | 1 cut(s) | 298 |
| PciI | ACATGT | 2 cut(s) | 768, 2021 |
| PciSI | GCTCTTC | 1 cut(s) | 2204 |
| PdmI | GAANNNNTTC | 2 cut(s) | 626, 1826 |
| PfeI | GAWTC | 4 cut(s) | 706, 758, 1211, 1580 |
| PflFI | GACNNNGTC | 1 cut(s) | 179 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 1189, 1936 |
| PfoI | TCCNGGA | 1 cut(s) | 304 |
| PkrI | GCNGC | 9 cut(s) | 108, 702, 905, 908, 978, 1615, 1793, 2151, 2257 |
| PleI | GAGTC | 5 cut(s) | 869, 956, 1155, 1738, 2052 |
| PpsI | GAGTC | 5 cut(s) | 869, 956, 1155, 1738, 2052 |
| Ppu21I | YACGTR | 1 cut(s) | 368 |
| PpuMI | RGGWCCY | 2 cut(s) | 17, 2047 |
| PscI | ACATGT | 2 cut(s) | 768, 2021 |
| PshBI | ATTAAT | 1 cut(s) | 1508 |
| PsiI | TTATAA | 1 cut(s) | 1476 |
| Psp124BI | GAGCTC | 1 cut(s) | 73 |
| Psp5II | RGGWCCY | 2 cut(s) | 17, 2047 |
| Psp6I | CCWGG | 2 cut(s) | 246, 1929 |
| PspEI | GGTNACC | 1 cut(s) | 1045 |
| PspFI | CCCAGC | 1 cut(s) | 1005 |
| PspGI | CCWGG | 2 cut(s) | 246, 1929 |
| PspN4I | GGNNCC | 3 cut(s) | 190, 675, 2048 |
| PspPI | GGNCC | 5 cut(s) | 17, 955, 1161, 1348, 2047 |
| PspPPI | RGGWCCY | 2 cut(s) | 17, 2047 |
| PstI | CTGCAG | 2 cut(s) | 2260, 2447 |
| PstNI | CAGNNNCTG | 1 cut(s) | 413 |
| PsuI | RGATCY | 2 cut(s) | 157, 2645 |
| PsyI | GACNNNGTC | 1 cut(s) | 179 |
| PvuII | CAGCTG | 4 cut(s) | 109, 906, 1005, 1616 |
| RsaI | GTAC | 4 cut(s) | 36, 52, 460, 553 |
| RsaNI | GTAC | 4 cut(s) | 35, 51, 459, 552 |
| RseI | CAYNNNNRTG | 2 cut(s) | 429, 818 |
| SacI | GAGCTC | 1 cut(s) | 73 |
| SapI | GCTCTTC | 1 cut(s) | 2204 |
| SatI | GCNGC | 9 cut(s) | 107, 701, 904, 907, 977, 1614, 1792, 2150, 2256 |
| Sau96I | GGNCC | 5 cut(s) | 17, 955, 1161, 1348, 2047 |
| ScaI | AGTACT | 1 cut(s) | 460 |
| SchI | GAGTC | 5 cut(s) | 869, 956, 1156, 1739, 2052 |
| ScrFI | CCNGG | 5 cut(s) | 248, 306, 491, 492, 1931 |
| SduI | GDGCHC | 4 cut(s) | 73, 719, 1672, 2037 |
| SfaNI | GCATC | 4 cut(s) | 531, 918, 1940, 2242 |
| SfcI | CTRYAG | 3 cut(s) | 405, 2256, 2443 |
| SfuI | TTCGAA | 1 cut(s) | 2164 |
| SinI | GGWCC | 4 cut(s) | 17, 955, 1161, 2047 |
| SmaI | CCCGGG | 1 cut(s) | 492 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 429, 818 |
| SmlI | CTYRAG | 5 cut(s) | 72, 193, 2030, 2234, 2377 |
| SmoI | CTYRAG | 5 cut(s) | 72, 193, 2030, 2234, 2377 |
| SsiI | CCGC | 5 cut(s) | 703, 871, 1445, 2344, 2597 |
| SspMI | CTAG | 7 cut(s) | 924, 1325, 1388, 1418, 1856, 2051, 2243 |
| SstI | GAGCTC | 1 cut(s) | 73 |
| StyD4I | CCNGG | 5 cut(s) | 246, 304, 489, 490, 1929 |
| StyI | CCWWGG | 5 cut(s) | 375, 555, 1140, 2050, 2641 |
| TaaI | ACNGT | 3 cut(s) | 431, 892, 1123 |
| TaiI | ACGT | 4 cut(s) | 289, 370, 1305, 2424 |
| TaqI | TCGA | 6 cut(s) | 253, 319, 567, 1760, 1830, 2164 |
| TaqII | GACCGA | 1 cut(s) | 1178 |
| TatI | WGTACW | 2 cut(s) | 34, 458 |
| TfiI | GAWTC | 4 cut(s) | 706, 758, 1211, 1580 |
| TscAI | CASTG | 6 cut(s) | 1113, 1274, 1337, 1606, 1879, 2583 |
| TseFI | GTSAC | 2 cut(s) | 496, 1731 |
| TseI | GCWGC | 9 cut(s) | 106, 700, 903, 906, 976, 1613, 1791, 2149, 2255 |
| Tsp45I | GTSAC | 2 cut(s) | 496, 1731 |
| TspGWI | ACGGA | 2 cut(s) | 700, 1276 |
| TspMI | CCCGGG | 1 cut(s) | 490 |
| TspRI | CASTG | 6 cut(s) | 1113, 1274, 1337, 1606, 1879, 2583 |
| Tth111I | GACNNNGTC | 1 cut(s) | 179 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 1189, 1936 |
| VneI | GTGCAC | 1 cut(s) | 715 |
| VpaK11BI | GGWCC | 4 cut(s) | 17, 955, 1161, 2047 |
| VspI | ATTAAT | 1 cut(s) | 1508 |
| XapI | RAATTY | 6 cut(s) | 467, 1178, 1470, 1535, 1715, 1957 |
| XceI | RCATGY | 4 cut(s) | 772, 1316, 1863, 2025 |
| XmaI | CCCGGG | 1 cut(s) | 490 |
| XmaJI | CCTAGG | 1 cut(s) | 2050 |
| XmiI | GTMKAC | 2 cut(s) | 1228, 2496 |
| XmnI | GAANNNNTTC | 2 cut(s) | 626, 1826 |
| XspI | CTAG | 7 cut(s) | 924, 1325, 1388, 1418, 1856, 2051, 2243 |
| ZrmI | AGTACT | 1 cut(s) | 460 |
| Zsp2I | ATGCAT | 1 cut(s) | 1955 |