Rw4G003000

NB-ARC domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0000540
Physical Location & Seq
Forward (+)
6005575 .. 6008085
2511 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G003000.1

Sequence Viewer

Length: 2475 bp
ATGTCGTTAGTAGTGGAGGCTATGGTCACGACTCTAACGGAGCATGTGTTCACAGCTCTAATCAACCAGGCTGAATTTGCACTTGACTTCAGCGGCCAGTTTGAGCAGTTGAAGACAGGGCTTGATCTCACCAAGGCCTTACTTGCAGACACGGAGAGTCTCAACCACAAGAACAACATTGTCAAGGCAGCATTATTTGAGCTGAGAGAAGTCATCTACAAAGCCGACGATGTACTCACTGATTGCTTGGTTAGAGATGAATACAGAAAGGATAGATCTTGTTCCGGCTCCTTGTTTCACGATCCATTCTTCCTGCATCGTACAGGAAAGAAATTGAAAGACATTAATTTGCGTATGAAGGAGATAGAGCAGACCTTGGGCAAGTTTTTGAGGGCTCCTGATAACATACATAGAGAAGATGCTTACCAAGTTAGGGTGATGGTTTCACAGGACTGGAATCCAACTGAGATAATTGGGTTGGATAGCGATGTGGAGAAGATTAAGGGTTGGCTTTTCGACACTAGCAAGGTACTTTCCCACGTAGGCATTGTGGGAATGGGAGGCTTGGGGAAAACCACCATTGCTCAAAAGATATTTCATGATCTAGAAGTAGTAGGCCACTTTCACAAGCTGATATGGGTGTGTGTTTCGCAATCATTCAGTGCTGATCGGATTATGAGGAGCATCCTAGAAGGTCTAGGAGAAAATGCCTCTGGAGCTGGTGTTACTCAGATTTTGAGCAGAATTCAGCAAGTACTTACAGATAAGAGCTGTCTGATTGTCATGGATGATGTGTGGAATCAAACAGATGTTGATTGGTGGACCAACCTCTGCTCTGTCATACCCAAACAAAGCAGCTGCATCATCGTTACAACTAGAAATGAAGAAGCTGTAATTGGTATGGGAGTTGAAAGCTCACGAATCCACCGTCCCAAGACTCTTAATGATGAGGAAAGCTGGCGCTTGTTCTCCAAGTTTGCATTTCCTACAAGCAAGGGATTGTGCCCGGAGGACAGGCTTGAGAAATTAGGAAAAGAACTCTTGAAGAAGTGTGGTGGACTCCCACTCGCAATCAAGACTATAGGATCCTTGTTGGCATCAAAGGTCGACTCCCCTTCTCAGTGGAGACGAGTTCTTGAGAGCTTTCATGCTTTGACAACAGAGGGAAAAACTAGCTCAGTTATGGCCTCTTTGCGATTGAGCTATGATGAACTCCCGCCTGGTCTGAAGCAATGCCTGCTGTGTTTCTCCATCTATCCTGAAGACTATGAGATACGTGCTGATCAGTTAATCCATTGGTGGATTGGAGAAGGCCTCGTGCAAAGCAAGGGCTCAAGGACAGCAGTAGAAGTGGGATATGAGTATCTTGCGGAACTGATAAGCAGATGCTTGGTTGAAATTGTGCAGCACAGAGGATTTGATGGGAGAGTCTACAAGTGCAAGATTCATGATATGGTCCGGGAACTGATAATAATGATTGCAGAGGAGGAGGAATTATGCGGCTTTGACGAACAAGGCAGACAGAAATTGACAGCAAATACTAGGTGGTTATGCTTTATTGATGAAATGGAGGAGAAGTCATTGAAACGTAGTTCAAAGCTCAGGGCATTGTTGATGATGTCAAGTCGCAGATTTGACTTTGAAAGGAATTCTCTGCGATCCCTCAGAATGTTGGATTTATCAAACTGCACTGTGGATGAGCATTGTCGCTTAGCAACTTTGAACTTAAGTGGTATTCAGGCTCTGAAAGAAGTGCCATCTTCAATTCATAAACTCCTCAACCTCCAACTATTACTTCTAACAGGTTGCAGCAATCTAGAAAAAATACACCCTTCCATCACTAATTTGAAGAAGCTAATTATTTTGGACCTAGGCGGGAGTCCAATCCAATACTTACCTCAGGGATTGGGAAGGCTTTCTTATCTTCAAGAGTTCTCTGGATTCAAGATTGTGAGTCAGCCTAGAATGCCATGTTTCCGGCTTCTTGAGATTAGGGAACTTATCAATCTGAGAGTACTGCGAATACATGTAAGCAGTACCACAGTTATTGCAGACAATGAACTTGATGTCCTCTCTCAGCTTAGAAGGCTCAATGTTCTGGCTATCGACGCAGAAGATTGTAGAAATAACAATGTTTTAGAAATGCTAGAAAGGCTGACCCCTCCTCCAAGCCTCCAAGAGTTGTATCTTAGGAACTACAAGAAGGAAACTCTGCCTAGCTGGGTTAATCCCGGACAGCTTTCAAGATTGCAGTATCTTTGCATCGAGAATGGAGACCTTGTCAAGCTAAGTAGTGGTCAAACCACCTGGAACCTTGAAGGCCTGTGTCTCAAGTACTTGATGAGGCTAGAGGTAGACTGGAAGGACTTGGAGAAGGATATGCCTGTACTCCGCTACATGGAGATCAGTCATTGTTACAACCTGAAGGATTTCCCATGTTCAGTTATGGAACCTGGGGTCTGGAGAAAGAATTAA

Protein Analysis

824

Amino Acids

94.01

Weight (kDa)

6.39

Isoelectric Point (pI)

43.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 9 - 95 1.3e-12 Rx N-terminal domain
NB-ARC PF00931 162 - 331 2.6e-40 NB-ARC domain
AAA_22 PF13401 183 - 265 5e-06 AAA domain
WHD_DRP PF23559 418 - 490 2.7e-20 Disease resistance protein Winged helix domain
LRR_14 PF23598 547 - 756 1e-24 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 676 - 765 2.6e-08 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000540)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G50950 AT3G50950
fragaria_vesca FvH4_1g12710 FvH4_1g12710 FvH4_3g00820 FvH4_3g00820 FvH4_3g00820 FvH4_3g00820
malus_domestica MD02G1141100.v1.1 MD10G1333100.v1.1
prunus_persica Prupe.4G009300_v2.0.a1 Prupe.4G009300_v2.0.a1 Prupe.4G009300_v2.0.a1 Prupe.6G150700_v2.0.a1 Prupe.7G160100_v2.0.a1 Prupe.7G160100_v2.0.a1
pyrus_communis pycom02g11110 pycom10g28240 pycom10g28340 pycom10g28360 pycom10g28450
rosa_chinensis RchiOBHm_Chr2g0100711 RchiOBHm_Chr3g0485861 RchiOBHm_Chr4g0391451 RchiOBHm_Chr4g0391591 RchiOBHm_Chr4g0391601 RchiOBHm_Chr4g0391681 RchiOBHm_Chr5g0001141 RchiOBHm_Chr5g0001221
rosa_laevigata RLG00000017001
rosa_multiflora Rmu_co8260007.1_g000001 Rmu_co8260103.1_g000001 Rmu_co8519155.1_g000001 Rmu_sc0000060.1_g000050 Rmu_sc0000255.1_g000036 Rmu_sc0001174.1_g000023 Rmu_sc0001174.1_g000025 Rmu_sc0001659.1_g000009 Rmu_sc0001706.1_g000009 Rmu_sc0001706.1_g000024 Rmu_sc0001706.1_g000035 Rmu_sc0004189.1_g000018 Rmu_sc0004189.1_g000030 Rmu_sc0004862.1_g000002 Rmu_sc0004876.1_g000038 Rmu_sc0023741.1_g000004
rosa_roxburghii Rroxscaffold_1G00075100 Rroxscaffold_1G00075160 Rroxscaffold_1G00075210 Rroxscaffold_5G00337160 Rroxscaffold_5G00386590 Rroxscaffold_6G00396300
rosa_rugosa Rorug03G0336200 Rorug03G0336300 Rorug04G0389400
rosa_samantha Rh2AG140400 Rh2BG144100 Rh2CG145400 Rh2DG144900 Rh3AG271200 Rh4AG038000 Rh4AG038700 Rh4BG032000 Rh4BG032800 Rh4CG041100 Rh4CG041300 Rh4DG035500 Rh4DG035600 Rh5AG008800 Rh5AG009100 Rh5BG010900 Rh5BG011500 Rh5BG011600 Rh5BG012000 Rh5CG009400 Rh5CG010000 Rh5DG009800
rosa_wichuraiana Rw2G010940 Rw4G002950 Rw4G002990 Rw4G003000 Rw5G000880 Rw5G000950 Rw5G000960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 3 cut(s) 1107, 1431, 2355
AciI CCGC 6 cut(s) 93, 1217, 1370, 1500, 1875, 2392
AclWI GGATC 4 cut(s) 296, 1080, 1093, 1653
AcoI YGGCCR 1 cut(s) 94
AcsI RAATTY 3 cut(s) 74, 744, 1648
AcuI CTGAAG 4 cut(s) 73, 1247, 1281, 2444
AfaI GTAC 8 cut(s) 234, 322, 531, 756, 2016, 2038, 2336, 2388
AfiI CCNNNNNNNGG 2 cut(s) 433, 2398
AflII CTTAAG 1 cut(s) 1726
AflIII ACRYGT 1 cut(s) 2026
AjnI CCWGG 4 cut(s) 66, 1219, 2306, 2452
AjuI GAANNNNNNNTTGG 4 cut(s) 879, 911, 1779, 1811
AloI GAACNNNNNNTCC 4 cut(s) 32, 64, 2052, 2084
Alw26I GTCTC 4 cut(s) 164, 1120, 2268, 2333
AlwI GGATC 4 cut(s) 296, 1080, 1093, 1653
AlwNI CAGNNNCTG 1 cut(s) 1744
AoxI GGCC 6 cut(s) 94, 135, 616, 1185, 1312, 2320
ApeKI GCWGC 5 cut(s) 188, 855, 858, 1405, 1809
ApoI RAATTY 3 cut(s) 74, 744, 1648
ArsI GACNNNNNNTTYG 2 cut(s) 913, 945
AseI ATTAAT 1 cut(s) 345
Asp700I GAANNNNTTC 2 cut(s) 1915, 2429
AspA2I CCTAGG 1 cut(s) 1870
AspLEI GCGC 1 cut(s) 963
AspS9I GGNCC 3 cut(s) 822, 1456, 1867
AsuC2I CCSGG 3 cut(s) 1007, 1460, 2232
AsuHPI GGTGA 2 cut(s) 121, 448
AvaII GGWCC 3 cut(s) 822, 1456, 1867
AvrII CCTAGG 1 cut(s) 1870
AxyI CCTNAGG 1 cut(s) 1899
BaeGI GKGCMC 1 cut(s) 1007
BamHI GGATCC 1 cut(s) 1085
BanII GRGCYC 2 cut(s) 397, 1334
BauI CACGAG 1 cut(s) 1316
BbsI GAAGAC 2 cut(s) 119, 1269
BbvI GCAGC 5 cut(s) 200, 845, 867, 1417, 1821
BccI CCATC 5 cut(s) 433, 1259, 1415, 1765, 1844
BciT130I CCWGG 4 cut(s) 68, 1221, 2308, 2454
BclI TGATCA 1 cut(s) 1282
BcnI CCSGG 3 cut(s) 1007, 1460, 2232
BcoDI GTCTC 4 cut(s) 164, 1120, 2268, 2333
BfmI CTRYAG 1 cut(s) 1080
BfoI RGCGCY 1 cut(s) 964
BfrI CTTAAG 1 cut(s) 1726
BglII AGATCT 1 cut(s) 275
BisI GCNGC 7 cut(s) 94, 189, 856, 859, 1406, 1501, 1810
BlnI CCTAGG 1 cut(s) 1870
BlpI GCTNAGC 1 cut(s) 1711
BlsI GCNGC 7 cut(s) 95, 190, 857, 860, 1407, 1502, 1811
BmcAI AGTACT 3 cut(s) 756, 2016, 2336
Bme1390I CCNGG 7 cut(s) 68, 1007, 1221, 1460, 2232, 2308, 2454
Bme18I GGWCC 3 cut(s) 822, 1456, 1867
BmgT120I GGNCC 3 cut(s) 822, 1456, 1867
BmiI GGNNCC 5 cut(s) 289, 396, 1087, 2312, 2451
BmrFI CCNGG 7 cut(s) 68, 1007, 1221, 1460, 2232, 2308, 2454
BmsI GCATC 7 cut(s) 325, 409, 693, 870, 1106, 1376, 2271
BpiI GAAGAC 2 cut(s) 119, 1269
BplI GAGNNNNNCTC 2 cut(s) 1299, 1331
BpmI CTGGAG 1 cut(s) 735
Bpu10I CCTNAGC 1 cut(s) 1601
Bpu1102I GCTNAGC 1 cut(s) 1711
BpuEI CTTGAG 5 cut(s) 1040, 1157, 1318, 2006, 2315
BpuMI CCSGG 3 cut(s) 1007, 1460, 2232
BsaAI YACGTR 2 cut(s) 541, 1277
BsaI GGTCTC 1 cut(s) 2268
BsaJI CCNNGG 4 cut(s) 132, 375, 1870, 2453
Bsc4I CCNNNNNNNGG 2 cut(s) 433, 2398
Bse1I ACTGG 3 cut(s) 97, 458, 2363
Bse21I CCTNAGG 1 cut(s) 1899
Bse3DI GCAATG 2 cut(s) 579, 1238
BseBI CCWGG 4 cut(s) 68, 1221, 2308, 2454
BseDI CCNNGG 4 cut(s) 132, 375, 1870, 2453
BseGI GGATG 3 cut(s) 684, 793, 1702
BseLI CCNNNNNNNGG 2 cut(s) 433, 2398
BseMI GCAATG 2 cut(s) 579, 1238
BseNI ACTGG 3 cut(s) 97, 458, 2363
BseRI GAGGAG 6 cut(s) 694, 1499, 1502, 1586, 1766, 2154
BseSI GKGCMC 1 cut(s) 1007
BseXI GCAGC 5 cut(s) 200, 845, 867, 1417, 1821
BseYI CCCAGC 1 cut(s) 2220
BsgI GTGCAG 2 cut(s) 1424, 1672
BshFI GGCC 6 cut(s) 96, 137, 618, 1187, 1314, 2322
BsiSI CCGG 5 cut(s) 285, 1007, 1459, 1978, 2232
BslFI GGGAC 1 cut(s) 915
BslI CCNNNNNNNGG 2 cut(s) 433, 2398
BsmAI GTCTC 4 cut(s) 164, 1120, 2268, 2333
BsmBI CGTCTC 1 cut(s) 1120
BsmFI GGGAC 1 cut(s) 915
BsmI GAATGC 1 cut(s) 1971
BsnI GGCC 6 cut(s) 96, 137, 618, 1187, 1314, 2322
Bso31I GGTCTC 1 cut(s) 2268
Bsp1286I GDGCHC 3 cut(s) 397, 1007, 1334
Bsp143I GATC 9 cut(s) 124, 275, 301, 601, 667, 1085, 1282, 1658, 2403
Bsp1720I GCTNAGC 1 cut(s) 1711
BspACI CCGC 6 cut(s) 93, 1217, 1370, 1500, 1875, 2392
BspANI GGCC 6 cut(s) 96, 137, 618, 1187, 1314, 2322
BspHI TCATGA 2 cut(s) 598, 1447
BspLI GGNNCC 5 cut(s) 289, 396, 1087, 2312, 2451
BspPI GGATC 4 cut(s) 296, 1080, 1093, 1653
BspTI CTTAAG 1 cut(s) 1726
BspTNI GGTCTC 1 cut(s) 2268
BsrDI GCAATG 2 cut(s) 579, 1238
BsrI ACTGG 3 cut(s) 97, 458, 2363
BssECI CCNNGG 4 cut(s) 132, 375, 1870, 2453
BssMI GATC 9 cut(s) 124, 275, 301, 601, 667, 1085, 1282, 1658, 2403
BssSI CACGAG 1 cut(s) 1316
BssT1I CCWWGG 3 cut(s) 132, 375, 1870
Bst2BI CACGAG 1 cut(s) 1316
Bst2UI CCWGG 4 cut(s) 68, 1221, 2308, 2454
Bst4CI ACNGT 3 cut(s) 929, 1693, 2044
BstAFI CTTAAG 1 cut(s) 1726
BstAPI GCANNNNNTGC 1 cut(s) 1237
BstBAI YACGTR 2 cut(s) 541, 1277
BstC8I GCNNGC 2 cut(s) 959, 1238
BstF5I GGATG 3 cut(s) 684, 793, 1702
BstH2I RGCGCY 1 cut(s) 964
BstHHI GCGC 1 cut(s) 963
BstKTI GATC 9 cut(s) 127, 278, 304, 604, 670, 1088, 1285, 1661, 2406
BstMAI GTCTC 4 cut(s) 164, 1120, 2268, 2333
BstMBI GATC 9 cut(s) 124, 275, 301, 601, 667, 1085, 1282, 1658, 2403
BstMWI GCNNNNNNNGC 8 cut(s) 77, 143, 716, 1237, 1966, 2086, 2108, 2152
BstNI CCWGG 4 cut(s) 68, 1221, 2308, 2454
BstNSI RCATGY 2 cut(s) 47, 2030
BstSCI CCNGG 7 cut(s) 66, 1005, 1219, 1458, 2230, 2306, 2452
BstSFI CTRYAG 1 cut(s) 1080
BstSLI GKGCMC 1 cut(s) 1007
BstV1I GCAGC 5 cut(s) 200, 845, 867, 1417, 1821
BstV2I GAAGAC 2 cut(s) 119, 1269
BstX2I RGATCY 2 cut(s) 275, 1085
BstYI RGATCY 2 cut(s) 275, 1085
Bsu36I CCTNAGG 1 cut(s) 1899
BsuRI GGCC 6 cut(s) 96, 137, 618, 1187, 1314, 2322
BtgZI GCGATG 1 cut(s) 501
BtsCI GGATG 3 cut(s) 684, 793, 1702
BtsIMutI CAGTG 4 cut(s) 237, 667, 1127, 1689
Cac8I GCNNGC 2 cut(s) 959, 1238
CaiI CAGNNNCTG 1 cut(s) 1744
CciI TCATGA 2 cut(s) 598, 1447
CfoI GCGC 1 cut(s) 963
Cfr13I GGNCC 3 cut(s) 822, 1456, 1867
CseI GACGC 1 cut(s) 2117
Csp6I GTAC 8 cut(s) 233, 321, 530, 755, 2015, 2037, 2335, 2387
CspCI CAANNNNNGTGG 2 cut(s) 2029, 2064
CviAII CATG 9 cut(s) 44, 599, 784, 1148, 1448, 1971, 2027, 2398, 2436
CviQI GTAC 8 cut(s) 233, 321, 530, 755, 2015, 2037, 2335, 2387
DpnI GATC 9 cut(s) 126, 277, 303, 603, 669, 1087, 1284, 1660, 2405
DpnII GATC 9 cut(s) 124, 275, 301, 601, 667, 1085, 1282, 1658, 2403
EaeI YGGCCR 1 cut(s) 94
Eco130I CCWWGG 3 cut(s) 132, 375, 1870
Eco147I AGGCCT 3 cut(s) 137, 1314, 2322
Eco24I GRGCYC 2 cut(s) 397, 1334
Eco31I GGTCTC 1 cut(s) 2268
Eco47I GGWCC 3 cut(s) 822, 1456, 1867
Eco57I CTGAAG 4 cut(s) 73, 1247, 1281, 2444
Eco81I CCTNAGG 1 cut(s) 1899
EcoRI GAATTC 2 cut(s) 744, 1648
EcoRII CCWGG 4 cut(s) 66, 1219, 2306, 2452
EcoT14I CCWWGG 3 cut(s) 132, 375, 1870
EcoT38I GRGCYC 2 cut(s) 397, 1334
ErhI CCWWGG 3 cut(s) 132, 375, 1870
Esp3I CGTCTC 1 cut(s) 1120
FaeI CATG 9 cut(s) 47, 602, 787, 1151, 1451, 1974, 2030, 2401, 2439
FalI AAGNNNNNCTT 2 cut(s) 1903, 1935
FaqI GGGAC 1 cut(s) 915
FatI CATG 9 cut(s) 43, 598, 783, 1147, 1447, 1970, 2026, 2397, 2435
FauI CCCGC 2 cut(s) 1224, 1868
FbaI TGATCA 1 cut(s) 1282
FblI GTMKAC 3 cut(s) 1107, 1431, 2355
Fnu4HI GCNGC 7 cut(s) 94, 189, 856, 859, 1406, 1501, 1810
FokI GGATG 3 cut(s) 671, 800, 1709
FriOI GRGCYC 2 cut(s) 397, 1334
Fsp4HI GCNGC 7 cut(s) 94, 189, 856, 859, 1406, 1501, 1810
GlaI GCGC 1 cut(s) 962
GluI GCNGC 7 cut(s) 94, 189, 856, 859, 1406, 1501, 1810
GsaI CCCAGC 1 cut(s) 2224
GsuI CTGGAG 1 cut(s) 735
HaeII RGCGCY 1 cut(s) 964
HaeIII GGCC 6 cut(s) 96, 137, 618, 1187, 1314, 2322
HapII CCGG 5 cut(s) 285, 1007, 1459, 1978, 2232
HgaI GACGC 1 cut(s) 2117
HhaI GCGC 1 cut(s) 963
Hin1II CATG 9 cut(s) 47, 602, 787, 1151, 1451, 1974, 2030, 2401, 2439
Hin6I GCGC 1 cut(s) 961
HinP1I GCGC 1 cut(s) 961
HincII GTYRAC 1 cut(s) 1108
HindII GTYRAC 1 cut(s) 1108
HpaII CCGG 5 cut(s) 285, 1007, 1459, 1978, 2232
HphI GGTGA 2 cut(s) 121, 448
Hpy166II GTNNAC 6 cut(s) 51, 822, 1058, 1108, 1432, 2356
Hpy188I TCNGA 7 cut(s) 672, 732, 777, 1227, 1667, 1746, 2010
Hpy8I GTNNAC 6 cut(s) 51, 822, 1058, 1108, 1432, 2356
Hpy99I CGWCG 2 cut(s) 230, 2111
HpyCH4III ACNGT 3 cut(s) 929, 1693, 2044
HpyCH4IV ACGT 3 cut(s) 540, 1276, 1588
HpyF10VI GCNNNNNNNGC 8 cut(s) 77, 143, 716, 1237, 1966, 2086, 2108, 2152
HpySE526I ACGT 3 cut(s) 540, 1276, 1588
Hsp92II CATG 9 cut(s) 47, 602, 787, 1151, 1451, 1974, 2030, 2401, 2439
HspAI GCGC 1 cut(s) 961
Ksp22I TGATCA 1 cut(s) 1282
Kzo9I GATC 9 cut(s) 124, 275, 301, 601, 667, 1085, 1282, 1658, 2403
LmnI GCTCC 5 cut(s) 40, 293, 400, 681, 716
Lsp1109I GCAGC 5 cut(s) 200, 845, 867, 1417, 1821
LweI GCATC 7 cut(s) 325, 409, 693, 870, 1106, 1376, 2271
MaeII ACGT 3 cut(s) 540, 1276, 1588
MaeIII GTNAC 4 cut(s) 25, 724, 868, 2414
MalI GATC 9 cut(s) 126, 277, 303, 603, 669, 1087, 1284, 1660, 2405
MboI GATC 9 cut(s) 124, 275, 301, 601, 667, 1085, 1282, 1658, 2403
MflI RGATCY 2 cut(s) 275, 1085
MhlI GDGCHC 3 cut(s) 397, 1007, 1334
MlyI GAGTC 8 cut(s) 25, 166, 931, 1053, 1103, 1437, 1888, 1963
MmeI TCCRAC 4 cut(s) 459, 485, 1653, 1810
MroXI GAANNNNTTC 2 cut(s) 1915, 2429
MseI TTAA 7 cut(s) 345, 501, 942, 1289, 1727, 2226, 2473
MspA1I CMGCKG 2 cut(s) 93, 858
MspCI CTTAAG 1 cut(s) 1726
MspI CCGG 5 cut(s) 285, 1007, 1459, 1978, 2232
MspR9I CCNGG 7 cut(s) 68, 1007, 1221, 1460, 2232, 2308, 2454
Mva1269I GAATGC 1 cut(s) 1971
MvaI CCWGG 4 cut(s) 68, 1221, 2308, 2454
MwoI GCNNNNNNNGC 8 cut(s) 77, 143, 716, 1237, 1966, 2086, 2108, 2152
NciI CCSGG 3 cut(s) 1007, 1460, 2232
NdeII GATC 9 cut(s) 124, 275, 301, 601, 667, 1085, 1282, 1658, 2403
NlaIII CATG 9 cut(s) 47, 602, 787, 1151, 1451, 1974, 2030, 2401, 2439
NlaIV GGNNCC 5 cut(s) 289, 396, 1087, 2312, 2451
NmuCI GTSAC 1 cut(s) 25
NspI RCATGY 2 cut(s) 47, 2030
PagI TCATGA 2 cut(s) 598, 1447
PceI AGGCCT 3 cut(s) 137, 1314, 2322
PciI ACATGT 1 cut(s) 2026
PcsI WCGNNNNNNNCGW 1 cut(s) 925
PctI GAATGC 1 cut(s) 1971
PdmI GAANNNNTTC 2 cut(s) 1915, 2429
PfeI GAWTC 5 cut(s) 457, 799, 921, 1444, 1941
PflFI GACNNNGTC 1 cut(s) 2279
PfoI TCCNGGA 2 cut(s) 1458, 2230
PkrI GCNGC 7 cut(s) 95, 190, 857, 860, 1407, 1502, 1811
PleI GAGTC 8 cut(s) 25, 165, 931, 1053, 1103, 1436, 1887, 1962
PpsI GAGTC 8 cut(s) 25, 165, 931, 1053, 1103, 1436, 1887, 1962
Ppu21I YACGTR 2 cut(s) 541, 1277
PscI ACATGT 1 cut(s) 2026
PshBI ATTAAT 1 cut(s) 345
Psp6I CCWGG 4 cut(s) 66, 1219, 2306, 2452
PspFI CCCAGC 1 cut(s) 2220
PspGI CCWGG 4 cut(s) 66, 1219, 2306, 2452
PspN4I GGNNCC 5 cut(s) 289, 396, 1087, 2312, 2451
PspPI GGNCC 3 cut(s) 822, 1456, 1867
PstNI CAGNNNCTG 1 cut(s) 1744
PsuI RGATCY 2 cut(s) 275, 1085
PsyI GACNNNGTC 1 cut(s) 2279
PvuII CAGCTG 1 cut(s) 858
RsaI GTAC 8 cut(s) 234, 322, 531, 756, 2016, 2038, 2336, 2388
RsaNI GTAC 8 cut(s) 233, 321, 530, 755, 2015, 2037, 2335, 2387
SalI GTCGAC 1 cut(s) 1106
SaqAI TTAA 7 cut(s) 345, 501, 942, 1289, 1727, 2226, 2473
SatI GCNGC 7 cut(s) 94, 189, 856, 859, 1406, 1501, 1810
Sau3AI GATC 9 cut(s) 124, 275, 301, 601, 667, 1085, 1282, 1658, 2403
Sau96I GGNCC 3 cut(s) 822, 1456, 1867
ScaI AGTACT 3 cut(s) 756, 2016, 2336
SchI GAGTC 8 cut(s) 25, 166, 931, 1053, 1103, 1437, 1888, 1963
ScrFI CCNGG 7 cut(s) 68, 1007, 1221, 1460, 2232, 2308, 2454
SduI GDGCHC 3 cut(s) 397, 1007, 1334
SfaNI GCATC 7 cut(s) 325, 409, 693, 870, 1106, 1376, 2271
SfcI CTRYAG 1 cut(s) 1080
SinI GGWCC 3 cut(s) 822, 1456, 1867
SmlI CTYRAG 6 cut(s) 1019, 1136, 1333, 1726, 1985, 2330
SmoI CTYRAG 6 cut(s) 1019, 1136, 1333, 1726, 1985, 2330
SseBI AGGCCT 3 cut(s) 137, 1314, 2322
SsiI CCGC 6 cut(s) 93, 1217, 1370, 1500, 1875, 2392
StuI AGGCCT 3 cut(s) 137, 1314, 2322
StyD4I CCNGG 7 cut(s) 66, 1005, 1219, 1458, 2230, 2306, 2452
StyI CCWWGG 3 cut(s) 132, 375, 1870
TaaI ACNGT 3 cut(s) 929, 1693, 2044
TaiI ACGT 3 cut(s) 543, 1279, 1591
TaqI TCGA 4 cut(s) 516, 1107, 2106, 2265
TatI WGTACW 5 cut(s) 232, 754, 2014, 2334, 2386
TauI GCSGC 2 cut(s) 96, 1503
TfiI GAWTC 5 cut(s) 457, 799, 921, 1444, 1941
Tru1I TTAA 7 cut(s) 345, 501, 942, 1289, 1727, 2226, 2473
Tru9I TTAA 7 cut(s) 345, 501, 942, 1289, 1727, 2226, 2473
TscAI CASTG 4 cut(s) 244, 667, 1127, 1696
TseFI GTSAC 1 cut(s) 25
TseI GCWGC 5 cut(s) 188, 855, 858, 1405, 1809
Tsp45I GTSAC 1 cut(s) 25
TspGWI ACGGA 2 cut(s) 53, 167
TspRI CASTG 4 cut(s) 244, 667, 1127, 1696
Tth111I GACNNNGTC 1 cut(s) 2279
Vha464I CTTAAG 1 cut(s) 1726
VpaK11BI GGWCC 3 cut(s) 822, 1456, 1867
VspI ATTAAT 1 cut(s) 345
XapI RAATTY 3 cut(s) 74, 744, 1648
XbaI TCTAGA 2 cut(s) 604, 1816
XceI RCATGY 2 cut(s) 47, 2030
XcmI CCANNNNNNNNNTGG 1 cut(s) 1301
XmaJI CCTAGG 1 cut(s) 1870
XmiI GTMKAC 3 cut(s) 1107, 1431, 2355
XmnI GAANNNNTTC 2 cut(s) 1915, 2429
ZrmI AGTACT 3 cut(s) 756, 2016, 2336
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.