Rh5BG011500

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0000540
Physical Location & Seq
Forward (+)
824120 .. 825700
1581 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG011500.1

Sequence Viewer

Length: 1446 bp
ATGGTCCAGACTCTAACGCAGCAAGTGTTCACAGCTCTAATGAACCAGGCTGAATTTGCACTCGACTTCAGCGGCCAGTTTGAGCAGTTGAAGACAGGGCTTGATCTCACCAGGGCCTTACTTGCAGACACGGAGAGTCTCAACCACAAGAACAACACTGTCAAGGCAGCATTGTTTGACTTAAGGGAAGTCATCTACAAAGCAGACGATGTACTCACTGATTGCTTGGTTAGAGATGAATACAGAAAGGATGGATCTTGTTCCGGTTCCTGGCTTCACGATCCATTCTTCCTGCATCGTACAGGAAAGAAATTGAGAGACATCAATTTGCGTATGAAGGAGATAGAGAAGACCTTGGGCCGGTTTTTGAGGGCACCAGACAGCATCCATAGAGAAGATGCATACCAAGTTAGAGTGATGGTGTCACAGGACTGGAATCCAACTGAGATAATTGGATTGGATAGCGATGTGGAGAAGATTAAGGGTTGGATTTTCGACACCAGCAACGCTGAGCGGATTATGAGGAGCATCCTAGAAGGTCTAGGAGAAAATGTCTCTGGAGCAAATGTTACTCAGATTTTGAGCAGAATCCAGCAAGTACTTAAAGATAAGAGCTGTTTGATTGTCATGGATGATGTGTGGAATCACACAGAATTTGATTGGTGGACCAATCTCTGCTCCGTTATACCCAAACAAAGCAGCTGCATCATAGTTACAACTAGAAATGAAGAAGCTGCAATTGGTATGGGAGTTGAAAGCTCACGAATCCACCGTCCCAAGACTCTTAATGATGAGGAAAGCTGGTGTTTATTCTCGAAGTTTGCATTTCCTACAAGCAAGGGATTGTGCCCGGAGGACAGGATTGAGAAATTAGGAAAAGAACTCTTGAAGAAGTGTGGTGGACTCCCGCTTGCAATTAAGACTATAGGATCCTTGTTGGCATCAAAGATCGACTCCCCTTCTCAGTGGAGGCGAGTTCTAGAGAGCTTTCATGCTTTGACAACCGAGGGAAAAACTAGCTCAGTTATGGCCTCTTTGCGATTGAGCTATGATGAACTCCCGCCTGGTCTGAAGCAATGCCTGCTGTGTTTCTCCATCTATCCTGAAGACTATGAGATACGTGCTGATCAGTTGATCCACTGGTGGATTGGAGAAGGCATCGTGCAAAGCAAGGGCTCAAGAACAGCAGTAGAAGTGGGATATGAGTATCTCGCAGAACTAATCAGCAGATGCTTGGTTGAAATTGTGCAGAGGAGAGGATTTGATGGGAGAGTCTACAAGTGCAAGATACACGATATGGTGCGGGAACTGATAATAATGATTGCAGAGGAAGAGGAGTTTTGCGGCTTTGACGAACGAGGTAGACAGGAATTGACAGCAAAGACTAGGTGGTTATGCTTTATTGATGAAATGGAGGAGAAGTCCTTGAAACGTAGTTCAAAGTGA

Protein Analysis

481

Amino Acids

55.06

Weight (kDa)

5.68

Isoelectric Point (pI)

44.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 1 - 86 1.4e-13 Rx N-terminal domain
NB-ARC PF00931 167 - 279 2.5e-19 NB-ARC domain
WHD_DRP PF23559 366 - 438 2.7e-20 Disease resistance protein Winged helix domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000540)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G50950 AT3G50950
fragaria_vesca FvH4_1g12710 FvH4_1g12710 FvH4_3g00820 FvH4_3g00820 FvH4_3g00820 FvH4_3g00820
malus_domestica MD02G1141100.v1.1 MD10G1333100.v1.1
prunus_persica Prupe.4G009300_v2.0.a1 Prupe.4G009300_v2.0.a1 Prupe.4G009300_v2.0.a1 Prupe.6G150700_v2.0.a1 Prupe.7G160100_v2.0.a1 Prupe.7G160100_v2.0.a1
pyrus_communis pycom02g11110 pycom10g28240 pycom10g28340 pycom10g28360 pycom10g28450
rosa_chinensis RchiOBHm_Chr2g0100711 RchiOBHm_Chr3g0485861 RchiOBHm_Chr4g0391451 RchiOBHm_Chr4g0391591 RchiOBHm_Chr4g0391601 RchiOBHm_Chr4g0391681 RchiOBHm_Chr5g0001141 RchiOBHm_Chr5g0001221
rosa_laevigata RLG00000017001
rosa_multiflora Rmu_co8260007.1_g000001 Rmu_co8260103.1_g000001 Rmu_co8519155.1_g000001 Rmu_sc0000060.1_g000050 Rmu_sc0000255.1_g000036 Rmu_sc0001174.1_g000023 Rmu_sc0001174.1_g000025 Rmu_sc0001659.1_g000009 Rmu_sc0001706.1_g000009 Rmu_sc0001706.1_g000024 Rmu_sc0001706.1_g000035 Rmu_sc0004189.1_g000018 Rmu_sc0004189.1_g000030 Rmu_sc0004862.1_g000002 Rmu_sc0004876.1_g000038 Rmu_sc0023741.1_g000004
rosa_roxburghii Rroxscaffold_1G00075100 Rroxscaffold_1G00075160 Rroxscaffold_1G00075210 Rroxscaffold_5G00337160 Rroxscaffold_5G00386590 Rroxscaffold_6G00396300
rosa_rugosa Rorug03G0336200 Rorug03G0336300 Rorug04G0389400
rosa_samantha Rh2AG140400 Rh2BG144100 Rh2CG145400 Rh2DG144900 Rh3AG271200 Rh4AG038000 Rh4AG038700 Rh4BG032000 Rh4BG032800 Rh4CG041100 Rh4CG041300 Rh4DG035500 Rh4DG035600 Rh5AG008800 Rh5AG009100 Rh5BG010900 Rh5BG011500 Rh5BG011600 Rh5BG012000 Rh5CG009400 Rh5CG010000 Rh5DG009800
rosa_wichuraiana Rw2G010940 Rw4G002950 Rw4G002990 Rw4G003000 Rw5G000880 Rw5G000950 Rw5G000960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 373
AccBSI CCGCTC 1 cut(s) 514
AccI GTMKAC 2 cut(s) 1275, 1363
AciI CCGC 6 cut(s) 72, 514, 908, 1061, 1303, 1344
AclWI GGATC 5 cut(s) 262, 275, 924, 937, 1129
AcoI YGGCCR 1 cut(s) 73
AcsI RAATTY 2 cut(s) 53, 653
AcuI CTGAAG 3 cut(s) 52, 1091, 1125
AfaI GTAC 3 cut(s) 213, 301, 600
AfiI CCNNNNNNNGG 2 cut(s) 270, 360
AflII CTTAAG 1 cut(s) 181
AgsI TTSAA 6 cut(s) 91, 755, 889, 1241, 1429, 1440
AjnI CCWGG 4 cut(s) 45, 110, 269, 1063
AjuI GAANNNNNNNTTGG 2 cut(s) 723, 755
AluBI AGCT 9 cut(s) 35, 615, 702, 734, 759, 801, 987, 1020, 1047
AluI AGCT 9 cut(s) 35, 615, 702, 734, 759, 801, 987, 1020, 1047
Alw26I GTCTC 3 cut(s) 143, 312, 559
AlwI GGATC 5 cut(s) 262, 275, 924, 937, 1129
AoxI GGCC 4 cut(s) 73, 114, 358, 1029
ApeKI GCWGC 5 cut(s) 19, 167, 699, 702, 734
ApoI RAATTY 2 cut(s) 53, 653
ArsI GACNNNNNNTTYG 2 cut(s) 757, 789
AspS9I GGNCC 4 cut(s) 4, 114, 358, 666
AsuC2I CCSGG 1 cut(s) 851
AsuHPI GGTGA 1 cut(s) 100
AvaII GGWCC 2 cut(s) 4, 666
BaeGI GKGCMC 2 cut(s) 376, 851
BamHI GGATCC 1 cut(s) 929
BanI GGYRCC 1 cut(s) 373
BanII GRGCYC 1 cut(s) 1178
BbsI GAAGAC 3 cut(s) 98, 356, 1113
BbvI GCAGC 5 cut(s) 31, 179, 689, 711, 721
BccI CCATC 4 cut(s) 245, 412, 1103, 1259
BciT130I CCWGG 4 cut(s) 47, 112, 271, 1065
BclI TGATCA 1 cut(s) 1126
BcnI CCSGG 1 cut(s) 851
BcoDI GTCTC 3 cut(s) 143, 312, 559
BfaI CTAG 6 cut(s) 533, 542, 720, 980, 1017, 1386
BfmI CTRYAG 1 cut(s) 924
BfrI CTTAAG 1 cut(s) 181
BisI GCNGC 7 cut(s) 20, 73, 168, 700, 703, 735, 1345
BlpI GCTNAGC 1 cut(s) 510
BlsI GCNGC 7 cut(s) 21, 74, 169, 701, 704, 736, 1346
BmcAI AGTACT 1 cut(s) 600
Bme1390I CCNGG 5 cut(s) 47, 112, 271, 851, 1065
Bme18I GGWCC 2 cut(s) 4, 666
BmgT120I GGNCC 4 cut(s) 4, 114, 358, 666
BmiI GGNNCC 3 cut(s) 268, 375, 931
BmrFI CCNGG 5 cut(s) 47, 112, 271, 851, 1065
BmsI GCATC 8 cut(s) 304, 388, 393, 537, 714, 950, 1167, 1220
BpiI GAAGAC 3 cut(s) 98, 356, 1113
BpmI CTGGAG 1 cut(s) 579
Bpu1102I GCTNAGC 1 cut(s) 510
BpuEI CTTGAG 1 cut(s) 1162
BpuMI CCSGG 1 cut(s) 851
BsaAI YACGTR 1 cut(s) 1121
BsaJI CCNNGG 3 cut(s) 111, 354, 1005
BsaWI WCCGGW 1 cut(s) 263
BsaXI ACNNNNNCTCC 6 cut(s) 537, 552, 567, 582, 1406, 1436
Bsc4I CCNNNNNNNGG 2 cut(s) 270, 360
Bse118I RCCGGY 1 cut(s) 360
Bse1I ACTGG 3 cut(s) 76, 437, 1145
Bse3DI GCAATG 1 cut(s) 1082
BseBI CCWGG 4 cut(s) 47, 112, 271, 1065
BseDI CCNNGG 3 cut(s) 111, 354, 1005
BseGI GGATG 4 cut(s) 256, 384, 528, 637
BseLI CCNNNNNNNGG 2 cut(s) 270, 360
BseMI GCAATG 1 cut(s) 1082
BseMII CTCAG 5 cut(s) 435, 501, 587, 977, 1035
BseNI ACTGG 3 cut(s) 76, 437, 1145
BseRI GAGGAG 4 cut(s) 538, 1267, 1349, 1430
BseSI GKGCMC 2 cut(s) 376, 851
BseXI GCAGC 5 cut(s) 31, 179, 689, 711, 721
BsgI GTGCAG 1 cut(s) 1268
BshFI GGCC 4 cut(s) 75, 116, 360, 1031
BshNI GGYRCC 1 cut(s) 373
BsiSI CCGG 3 cut(s) 264, 361, 851
BslFI GGGAC 1 cut(s) 759
BslI CCNNNNNNNGG 2 cut(s) 270, 360
BsmAI GTCTC 3 cut(s) 143, 312, 559
BsmFI GGGAC 1 cut(s) 759
BsnI GGCC 4 cut(s) 75, 116, 360, 1031
Bsp1286I GDGCHC 3 cut(s) 376, 851, 1178
Bsp143I GATC 7 cut(s) 103, 254, 280, 929, 948, 1126, 1134
Bsp1720I GCTNAGC 1 cut(s) 510
BspACI CCGC 6 cut(s) 72, 514, 908, 1061, 1303, 1344
BspANI GGCC 4 cut(s) 75, 116, 360, 1031
BspCNI CTCAG 5 cut(s) 436, 502, 586, 976, 1034
BspLI GGNNCC 3 cut(s) 268, 375, 931
BspPI GGATC 5 cut(s) 262, 275, 924, 937, 1129
BspT107I GGYRCC 1 cut(s) 373
BspTI CTTAAG 1 cut(s) 181
BsrBI CCGCTC 1 cut(s) 514
BsrDI GCAATG 1 cut(s) 1082
BsrFI RCCGGY 1 cut(s) 360
BsrI ACTGG 3 cut(s) 76, 437, 1145
BssAI RCCGGY 1 cut(s) 360
BssECI CCNNGG 3 cut(s) 111, 354, 1005
BssMI GATC 7 cut(s) 103, 254, 280, 929, 948, 1126, 1134
BssT1I CCWWGG 1 cut(s) 354
Bst2UI CCWGG 4 cut(s) 47, 112, 271, 1065
Bst4CI ACNGT 2 cut(s) 160, 773
Bst6I CTCTTC 1 cut(s) 1326
BstAFI CTTAAG 1 cut(s) 181
BstAPI GCANNNNNTGC 1 cut(s) 1081
BstBAI YACGTR 1 cut(s) 1121
BstC8I GCNNGC 2 cut(s) 912, 1082
BstDEI CTNAG 5 cut(s) 444, 510, 573, 963, 1021
BstF5I GGATG 4 cut(s) 256, 384, 528, 637
BstKTI GATC 7 cut(s) 106, 257, 283, 932, 951, 1129, 1137
BstMAI GTCTC 3 cut(s) 143, 312, 559
BstMBI GATC 7 cut(s) 103, 254, 280, 929, 948, 1126, 1134
BstMWI GCNNNNNNNGC 3 cut(s) 56, 122, 1081
BstNI CCWGG 4 cut(s) 47, 112, 271, 1065
BstSCI CCNGG 5 cut(s) 45, 110, 269, 849, 1063
BstSFI CTRYAG 1 cut(s) 924
BstSLI GKGCMC 2 cut(s) 376, 851
BstV1I GCAGC 5 cut(s) 31, 179, 689, 711, 721
BstV2I GAAGAC 3 cut(s) 98, 356, 1113
BstX2I RGATCY 2 cut(s) 254, 929
BstYI RGATCY 2 cut(s) 254, 929
BsuRI GGCC 4 cut(s) 75, 116, 360, 1031
BtgZI GCGATG 1 cut(s) 480
BtsCI GGATG 4 cut(s) 256, 384, 528, 637
BtsIMutI CAGTG 4 cut(s) 156, 216, 971, 1138
Cac8I GCNNGC 2 cut(s) 912, 1082
Cfr10I RCCGGY 1 cut(s) 360
Cfr13I GGNCC 4 cut(s) 4, 114, 358, 666
Csp6I GTAC 3 cut(s) 212, 300, 599
CviAII CATG 2 cut(s) 628, 992
CviQI GTAC 3 cut(s) 212, 300, 599
DdeI CTNAG 5 cut(s) 444, 510, 573, 963, 1021
DpnI GATC 7 cut(s) 105, 256, 282, 931, 950, 1128, 1136
DpnII GATC 7 cut(s) 103, 254, 280, 929, 948, 1126, 1134
EaeI YGGCCR 1 cut(s) 73
Eam1104I CTCTTC 1 cut(s) 1326
EarI CTCTTC 1 cut(s) 1326
Eco130I CCWWGG 1 cut(s) 354
Eco24I GRGCYC 1 cut(s) 1178
Eco47I GGWCC 2 cut(s) 4, 666
Eco57I CTGAAG 3 cut(s) 52, 1091, 1125
EcoO109I RGGNCCY 1 cut(s) 114
EcoRII CCWGG 4 cut(s) 45, 110, 269, 1063
EcoT14I CCWWGG 1 cut(s) 354
EcoT22I ATGCAT 1 cut(s) 403
EcoT38I GRGCYC 1 cut(s) 1178
ErhI CCWWGG 1 cut(s) 354
FaeI CATG 2 cut(s) 631, 995
FaqI GGGAC 1 cut(s) 759
FatI CATG 2 cut(s) 627, 991
FauI CCCGC 3 cut(s) 915, 1068, 1296
FbaI TGATCA 1 cut(s) 1126
FblI GTMKAC 2 cut(s) 1275, 1363
Fnu4HI GCNGC 7 cut(s) 20, 73, 168, 700, 703, 735, 1345
FokI GGATG 4 cut(s) 263, 371, 515, 644
FriOI GRGCYC 1 cut(s) 1178
Fsp4HI GCNGC 7 cut(s) 20, 73, 168, 700, 703, 735, 1345
FspBI CTAG 6 cut(s) 533, 542, 720, 980, 1017, 1386
GluI GCNGC 7 cut(s) 20, 73, 168, 700, 703, 735, 1345
GsuI CTGGAG 1 cut(s) 579
HaeIII GGCC 4 cut(s) 75, 116, 360, 1031
HapII CCGG 3 cut(s) 264, 361, 851
Hin1II CATG 2 cut(s) 631, 995
HpaII CCGG 3 cut(s) 264, 361, 851
HphI GGTGA 1 cut(s) 100
Hpy166II GTNNAC 5 cut(s) 30, 666, 902, 1276, 1364
Hpy188I TCNGA 2 cut(s) 576, 1071
Hpy188III TCNNGA 9 cut(s) 7, 278, 558, 762, 814, 886, 980, 1103, 1179
Hpy8I GTNNAC 5 cut(s) 30, 666, 902, 1276, 1364
HpyAV CCTTC 4 cut(s) 331, 530, 969, 1148
HpyCH4III ACNGT 2 cut(s) 160, 773
HpyCH4IV ACGT 2 cut(s) 1120, 1432
HpyF10VI GCNNNNNNNGC 3 cut(s) 56, 122, 1081
HpyF3I CTNAG 5 cut(s) 444, 510, 573, 963, 1021
HpySE526I ACGT 2 cut(s) 1120, 1432
Hsp92II CATG 2 cut(s) 631, 995
Ksp22I TGATCA 1 cut(s) 1126
Kzo9I GATC 7 cut(s) 103, 254, 280, 929, 948, 1126, 1134
LmnI GCTCC 3 cut(s) 525, 560, 683
Lsp1109I GCAGC 5 cut(s) 31, 179, 689, 711, 721
LweI GCATC 8 cut(s) 304, 388, 393, 537, 714, 950, 1167, 1220
MaeI CTAG 6 cut(s) 533, 542, 720, 980, 1017, 1386
MaeII ACGT 2 cut(s) 1120, 1432
MaeIII GTNAC 3 cut(s) 423, 568, 712
MalI GATC 7 cut(s) 105, 256, 282, 931, 950, 1128, 1136
MbiI CCGCTC 1 cut(s) 514
MboI GATC 7 cut(s) 103, 254, 280, 929, 948, 1126, 1134
MboII GAAGA 9 cut(s) 103, 280, 361, 407, 487, 740, 901, 1118, 1343
MfeI CAATTG 1 cut(s) 738
MflI RGATCY 2 cut(s) 254, 929
MhlI GDGCHC 3 cut(s) 376, 851, 1178
MlyI GAGTC 6 cut(s) 4, 145, 775, 897, 947, 1281
MmeI TCCRAC 2 cut(s) 464, 467
Mph1103I ATGCAT 1 cut(s) 403
MseI TTAA 5 cut(s) 182, 480, 603, 786, 918
MspA1I CMGCKG 2 cut(s) 72, 702
MspCI CTTAAG 1 cut(s) 181
MspI CCGG 3 cut(s) 264, 361, 851
MspR9I CCNGG 5 cut(s) 47, 112, 271, 851, 1065
MunI CAATTG 1 cut(s) 738
MvaI CCWGG 4 cut(s) 47, 112, 271, 1065
MwoI GCNNNNNNNGC 3 cut(s) 56, 122, 1081
NciI CCSGG 1 cut(s) 851
NdeII GATC 7 cut(s) 103, 254, 280, 929, 948, 1126, 1134
NlaIII CATG 2 cut(s) 631, 995
NlaIV GGNNCC 3 cut(s) 268, 375, 931
NmuCI GTSAC 1 cut(s) 423
NsiI ATGCAT 1 cut(s) 403
PcsI WCGNNNNNNNCGW 1 cut(s) 769
PfeI GAWTC 4 cut(s) 436, 588, 643, 765
PkrI GCNGC 7 cut(s) 21, 74, 169, 701, 704, 736, 1346
PleI GAGTC 6 cut(s) 4, 144, 775, 897, 947, 1280
PpsI GAGTC 6 cut(s) 4, 144, 775, 897, 947, 1280
Ppu21I YACGTR 1 cut(s) 1121
Psp6I CCWGG 4 cut(s) 45, 110, 269, 1063
PspGI CCWGG 4 cut(s) 45, 110, 269, 1063
PspN4I GGNNCC 3 cut(s) 268, 375, 931
PspPI GGNCC 4 cut(s) 4, 114, 358, 666
PsuI RGATCY 2 cut(s) 254, 929
PvuII CAGCTG 1 cut(s) 702
RsaI GTAC 3 cut(s) 213, 301, 600
RsaNI GTAC 3 cut(s) 212, 300, 599
SaqAI TTAA 5 cut(s) 182, 480, 603, 786, 918
SatI GCNGC 7 cut(s) 20, 73, 168, 700, 703, 735, 1345
Sau3AI GATC 7 cut(s) 103, 254, 280, 929, 948, 1126, 1134
Sau96I GGNCC 4 cut(s) 4, 114, 358, 666
ScaI AGTACT 1 cut(s) 600
SchI GAGTC 6 cut(s) 4, 145, 775, 897, 947, 1281
ScrFI CCNGG 5 cut(s) 47, 112, 271, 851, 1065
SduI GDGCHC 3 cut(s) 376, 851, 1178
SfaNI GCATC 8 cut(s) 304, 388, 393, 537, 714, 950, 1167, 1220
SfcI CTRYAG 1 cut(s) 924
SinI GGWCC 2 cut(s) 4, 666
SmlI CTYRAG 2 cut(s) 181, 1177
SmoI CTYRAG 2 cut(s) 181, 1177
SsiI CCGC 6 cut(s) 72, 514, 908, 1061, 1303, 1344
SspMI CTAG 6 cut(s) 533, 542, 720, 980, 1017, 1386
StyD4I CCNGG 5 cut(s) 45, 110, 269, 849, 1063
StyI CCWWGG 1 cut(s) 354
TaaI ACNGT 2 cut(s) 160, 773
TaiI ACGT 2 cut(s) 1123, 1435
TaqI TCGA 4 cut(s) 63, 495, 815, 951
TatI WGTACW 2 cut(s) 211, 598
TauI GCSGC 2 cut(s) 75, 1347
TfiI GAWTC 4 cut(s) 436, 588, 643, 765
Tru1I TTAA 5 cut(s) 182, 480, 603, 786, 918
Tru9I TTAA 5 cut(s) 182, 480, 603, 786, 918
TscAI CASTG 4 cut(s) 163, 223, 971, 1145
TseFI GTSAC 1 cut(s) 423
TseI GCWGC 5 cut(s) 19, 167, 699, 702, 734
Tsp45I GTSAC 1 cut(s) 423
TspDTI ATGAA 7 cut(s) 56, 252, 350, 741, 980, 1068, 1422
TspGWI ACGGA 2 cut(s) 146, 670
TspRI CASTG 4 cut(s) 163, 223, 971, 1145
Vha464I CTTAAG 1 cut(s) 181
VpaK11BI GGWCC 2 cut(s) 4, 666
XapI RAATTY 2 cut(s) 53, 653
XbaI TCTAGA 1 cut(s) 979
XcmI CCANNNNNNNNNTGG 1 cut(s) 1145
XmiI GTMKAC 2 cut(s) 1275, 1363
XspI CTAG 6 cut(s) 533, 542, 720, 980, 1017, 1386
ZrmI AGTACT 1 cut(s) 600
Zsp2I ATGCAT 1 cut(s) 403
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.