Rw4G002990

NB-ARC domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0000540
Physical Location & Seq
Forward (+)
5942044 .. 5944554
2511 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G002990.1

Sequence Viewer

Length: 2424 bp
ATGTCGTTAATAGTGGGGGCTATGGTCCAGACTCTAACGGAGCATGTGTTCACAGCTCTAATGAACCAGGCTGAATTTGCACTTGACTTCAGCGGCCAGTTTGAGCAGTTGAAGACGGGGCTTGATGTCACCAAGGCGTTACTTGCAGACACGGAGAGTCTAAACCACAAGAACAACATTGTCAAGGCAGCAGTATTTGAGCTGAGAGAAGTTATCTACAAAGCCGACTATGTACTCACTGATTGCTTGGTTAGAGACGTATACAGAAAGGATGGATCTTGTTCCGGCTCCTTGTTTCACGATCCATTCTTCCTGCATCGTACGGGAAAGAAATTGAAAGACATCAATTTGCGTATGAAGGAGATAGAGCAGACCTTGGGCAAGGTGATGGTTTCACAGGACTGGAATCCAACTGAGATAATTGGGTTGGATAGCGATGTGGAGAAGATTAAGGGTTGGCTTTTCGACACTAGCAAGGTACTTTCCCACGTAGGCATTGTGGGAATGGGAGGCTTGGGGAAAACCACCATTGCTCAAAAGATATTTCATGATCTAGAAGTAGTAGACCACTTTCACAAGCTGATATGGGTGTGTGTTTCACAATCATTCAGTGCTGATCGGATTATGAGGAGCATACTAGAAGGTCTAGGAGAAAATGTCTCCGGAGCTGGTGTTACTCAGATTTTGAGCAGAATTCAGCAAGTACTTGAAGATAAGAGTTGTTTGATTGTCATGGATGATGTGTGGAATCAAACAGATATTGATTGGTGGACCAACCTCTGCTCTGTTATACCCAAACAACGCAGCTGCATCATCGTTACAACTAGAAATGAAGAAGCTGTAATTGGTATGGGAGTTGAAAGCTCACGAATCCACCGTCCCAAGACTCTTAATGATGAGGAAAGCTGGCGCTTGTTCTCCAAGTTTGCATTTCCTACAAGCAAGGGATTGTGCCCAGAAGATAGGCTTGAGAAATTAGGAAAAGAACTCTTGAAGAAGTGTGGTGGACTCCCACTCGCAATCAAGACTATAGGATCCTTGTTGGCATCAAAGGTCGACTCCCCTTCTCAGTGGAGACGAGTTCTGGAGAGCTTTCATGCTTTGACAACAGAGGGAAAAACTAGCTCAGTTATGGCCTCTTTGCGATTGAGCTATGATGAACTCCCGCCCGGTCTGAAGCAATGCCTGCTGTGTTTCTCCATCTATCCAGAAGACTATGAGATACGTGCTGATCAGTTAATCCATTGGTGGATTGGAGAAGGCCTCGTGCAAAGCAAGGGCTCAAGAACAGCAGTAGAAGTGGGATATGAGTATCTTGCGGAACTGATAAGCAGATGCTTGGTTGAAATTGTGCAGCAGAGAGGATTTGATGGGAGAGTCTACAAGTGCAAGATTCATGATATGGTCCGGGAACTGATAATAATGATTGCAGAGGAGGAGGAATTATGCGGCTTTGACGAACAAGGCAGACAGAAATTGACGGCAAATACTAGGTGGCTATGCTTTATTGATGAAATGGAGGAGAAGTCCTTGAAACGTAGTTCAAAGCTCAGGGCATTGTTGATGATGTCAAGCCGCAGATTTGACTTTGAAAGGAATTCTTTACGATCCCTCAGAATGTTGGATTTATCAAACTGCACTGTGGATGAGCATTGTCGCTTAGCATCTTTGAACTTAAGTGGTATTCAGGCTCTGAAAGAACTGCCATCTTCAATTCATAAACTCCTCAACCTCCAACTTTTACTTCTAACAGGTTGCAGCAATCTAGAAAAAATACACCCTTCCATCACTAATTTGAAGAAGCTGATTATTTTGGACCTAGGCGGGTGTCCAATCCAATACTTACCTCAGGGATTGGGAAGGCTTTCTTATCTTCAAGAGTTCTCCGGATTCAAGATTGTGAGTCAGCCCAGAATGCCATGTTTCCGGCTTCTTGAGATTAGGGAACTGATCAACCTGAGAGTACTGCGAATACATGTAAGCAGTACCACAGTTATTGCAGACAATGAACTTGATGTCCTCTCTCAGCTTCGAAGGCTCAATGTTCTGGCTATCGACGCAGAAGATTGTAGAAATAACAATGTTTTGGAAATGCTAGAAAGGCTGACCCCTCCTCCAAGCCTCCAAGAGCTGTATCTTAGGAATTACAAGAAGGAAACTCTGCCTAGCTGGGTTAATCCCGGACAGCTTTCAAGATTGCAGTATCTTTGCATCGAGAATGGAGACCTTGTCAAGCTAAGTAGTGGTCAAACCTCCTGGAACCTTGAAGGCCTGTGTCTCAAGTACTTGATGAGGCTAGAGGTAGACTGGAAGGACTTGGAGAAGGATACGCCTGTACTCCACTACATGGAGATCAGTCATTGTTACAACTTGAAGGATTTCCCATGTTCAGTTATGGAACCAGGGGTCTGGAGAAAGAATTAA

Protein Analysis

807

Amino Acids

91.94

Weight (kDa)

6.36

Isoelectric Point (pI)

41.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 8 - 91 1.6e-10 Rx N-terminal domain
NB-ARC PF00931 145 - 314 2.1e-40 NB-ARC domain
AAA_22 PF13401 166 - 248 4.1e-06 AAA domain
WHD_DRP PF23559 401 - 473 2.1e-20 Disease resistance protein Winged helix domain
LRR_14 PF23598 530 - 739 3.6e-25 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 659 - 747 1.7e-08 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000540)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G50950 AT3G50950
fragaria_vesca FvH4_1g12710 FvH4_1g12710 FvH4_3g00820 FvH4_3g00820 FvH4_3g00820 FvH4_3g00820
malus_domestica MD02G1141100.v1.1 MD10G1333100.v1.1
prunus_persica Prupe.4G009300_v2.0.a1 Prupe.4G009300_v2.0.a1 Prupe.4G009300_v2.0.a1 Prupe.6G150700_v2.0.a1 Prupe.7G160100_v2.0.a1 Prupe.7G160100_v2.0.a1
pyrus_communis pycom02g11110 pycom10g28240 pycom10g28340 pycom10g28360 pycom10g28450
rosa_chinensis RchiOBHm_Chr2g0100711 RchiOBHm_Chr3g0485861 RchiOBHm_Chr4g0391451 RchiOBHm_Chr4g0391591 RchiOBHm_Chr4g0391601 RchiOBHm_Chr4g0391681 RchiOBHm_Chr5g0001141 RchiOBHm_Chr5g0001221
rosa_laevigata RLG00000017001
rosa_multiflora Rmu_co8260007.1_g000001 Rmu_co8260103.1_g000001 Rmu_co8519155.1_g000001 Rmu_sc0000060.1_g000050 Rmu_sc0000255.1_g000036 Rmu_sc0001174.1_g000023 Rmu_sc0001174.1_g000025 Rmu_sc0001659.1_g000009 Rmu_sc0001706.1_g000009 Rmu_sc0001706.1_g000024 Rmu_sc0001706.1_g000035 Rmu_sc0004189.1_g000018 Rmu_sc0004189.1_g000030 Rmu_sc0004862.1_g000002 Rmu_sc0004876.1_g000038 Rmu_sc0023741.1_g000004
rosa_roxburghii Rroxscaffold_1G00075100 Rroxscaffold_1G00075160 Rroxscaffold_1G00075210 Rroxscaffold_5G00337160 Rroxscaffold_5G00386590 Rroxscaffold_6G00396300
rosa_rugosa Rorug03G0336200 Rorug03G0336300 Rorug04G0389400
rosa_samantha Rh2AG140400 Rh2BG144100 Rh2CG145400 Rh2DG144900 Rh3AG271200 Rh4AG038000 Rh4AG038700 Rh4BG032000 Rh4BG032800 Rh4CG041100 Rh4CG041300 Rh4DG035500 Rh4DG035600 Rh5AG008800 Rh5AG009100 Rh5BG010900 Rh5BG011500 Rh5BG011600 Rh5BG012000 Rh5CG009400 Rh5CG010000 Rh5DG009800
rosa_wichuraiana Rw2G010940 Rw4G002950 Rw4G002990 Rw4G003000 Rw5G000880 Rw5G000950 Rw5G000960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 2347
AccI GTMKAC 5 cut(s) 261, 564, 1056, 1380, 2304
AccIII TCCGGA 2 cut(s) 662, 1886
AciI CCGC 6 cut(s) 93, 1166, 1319, 1449, 1576, 1824
AclWI GGATC 5 cut(s) 283, 296, 1029, 1042, 1602
AcoI YGGCCR 1 cut(s) 94
AcsI RAATTY 3 cut(s) 74, 693, 1597
AcuI CTGAAG 2 cut(s) 73, 1196
AfaI GTAC 8 cut(s) 234, 322, 480, 705, 1965, 1987, 2285, 2337
AfiI CCNNNNNNNGG 1 cut(s) 2347
AflII CTTAAG 1 cut(s) 1675
AflIII ACRYGT 1 cut(s) 1975
AjnI CCWGG 3 cut(s) 66, 2255, 2401
AjuI GAANNNNNNNTTGG 4 cut(s) 828, 860, 1728, 1760
AloI GAACNNNNNNTCC 4 cut(s) 32, 64, 2001, 2033
Alw26I GTCTC 5 cut(s) 249, 664, 1069, 2217, 2282
AlwI GGATC 5 cut(s) 283, 296, 1029, 1042, 1602
AlwNI CAGNNNCTG 1 cut(s) 1693
Aor13HI TCCGGA 2 cut(s) 662, 1886
AoxI GGCC 4 cut(s) 94, 1134, 1261, 2269
ApeKI GCWGC 5 cut(s) 188, 804, 807, 1354, 1758
ApoI RAATTY 3 cut(s) 74, 693, 1597
ArsI GACNNNNNNTTYG 2 cut(s) 862, 894
Asp700I GAANNNNTTC 2 cut(s) 1864, 2378
AspA2I CCTAGG 1 cut(s) 1819
AspLEI GCGC 1 cut(s) 912
AspS9I GGNCC 4 cut(s) 25, 771, 1405, 1816
AsuC2I CCSGG 3 cut(s) 1170, 1409, 2181
AsuHPI GGTGA 2 cut(s) 121, 397
AsuII TTCGAA 1 cut(s) 2032
AvaII GGWCC 4 cut(s) 25, 771, 1405, 1816
AvrII CCTAGG 1 cut(s) 1819
AxyI CCTNAGG 1 cut(s) 1848
BaeGI GKGCMC 1 cut(s) 956
BaeI ACNNNNGTAYC 2 cut(s) 2319, 2352
BamHI GGATCC 1 cut(s) 1034
BanII GRGCYC 1 cut(s) 1283
BauI CACGAG 1 cut(s) 1265
BbsI GAAGAC 2 cut(s) 119, 1218
BbvI GCAGC 5 cut(s) 200, 794, 816, 1366, 1770
BccI CCATC 6 cut(s) 266, 382, 1208, 1364, 1714, 1793
BceAI ACGGC 1 cut(s) 1497
BciT130I CCWGG 3 cut(s) 68, 2257, 2403
BciVI GTATCC 1 cut(s) 2320
BclI TGATCA 2 cut(s) 1231, 1950
BcnI CCSGG 3 cut(s) 1170, 1409, 2181
BcoDI GTCTC 5 cut(s) 249, 664, 1069, 2217, 2282
BfmI CTRYAG 1 cut(s) 1029
BfoI RGCGCY 1 cut(s) 913
BfrI CTTAAG 1 cut(s) 1675
BfuI GTATCC 1 cut(s) 2320
BisI GCNGC 8 cut(s) 94, 189, 805, 808, 1355, 1450, 1576, 1759
BlnI CCTAGG 1 cut(s) 1819
BlpI GCTNAGC 1 cut(s) 1660
BlsI GCNGC 8 cut(s) 95, 190, 806, 809, 1356, 1451, 1577, 1760
BmcAI AGTACT 3 cut(s) 705, 1965, 2285
Bme1390I CCNGG 6 cut(s) 68, 1170, 1409, 2181, 2257, 2403
Bme18I GGWCC 4 cut(s) 25, 771, 1405, 1816
BmgT120I GGNCC 4 cut(s) 25, 771, 1405, 1816
BmiI GGNNCC 4 cut(s) 289, 1036, 2261, 2400
BmrFI CCNGG 6 cut(s) 68, 1170, 1409, 2181, 2257, 2403
BmsI GCATC 6 cut(s) 325, 819, 1055, 1325, 1673, 2220
BpiI GAAGAC 2 cut(s) 119, 1218
BplI GAGNNNNNCTC 2 cut(s) 1248, 1280
BpmI CTGGAG 1 cut(s) 1106
Bpu10I CCTNAGC 1 cut(s) 1550
Bpu1102I GCTNAGC 1 cut(s) 1660
Bpu14I TTCGAA 1 cut(s) 2032
BpuEI CTTGAG 4 cut(s) 989, 1267, 1955, 2264
BpuMI CCSGG 3 cut(s) 1170, 1409, 2181
BsaAI YACGTR 2 cut(s) 490, 1226
BsaI GGTCTC 1 cut(s) 2217
BsaJI CCNNGG 4 cut(s) 132, 375, 1819, 2402
BsaWI WCCGGW 2 cut(s) 662, 1886
Bsc4I CCNNNNNNNGG 1 cut(s) 2347
Bse1I ACTGG 3 cut(s) 97, 407, 2312
Bse21I CCTNAGG 1 cut(s) 1848
Bse3DI GCAATG 2 cut(s) 528, 1187
BseAI TCCGGA 2 cut(s) 662, 1886
BseBI CCWGG 3 cut(s) 68, 2257, 2403
BseDI CCNNGG 4 cut(s) 132, 375, 1819, 2402
BseGI GGATG 3 cut(s) 277, 742, 1651
BseLI CCNNNNNNNGG 1 cut(s) 2347
BseMI GCAATG 2 cut(s) 528, 1187
BseNI ACTGG 3 cut(s) 97, 407, 2312
BseRI GAGGAG 6 cut(s) 643, 1448, 1451, 1535, 1715, 2103
BseSI GKGCMC 1 cut(s) 956
BseXI GCAGC 5 cut(s) 200, 794, 816, 1366, 1770
BseYI CCCAGC 1 cut(s) 2169
BsgI GTGCAG 2 cut(s) 1373, 1621
BshFI GGCC 4 cut(s) 96, 1136, 1263, 2271
BsiSI CCGG 7 cut(s) 285, 663, 1170, 1408, 1887, 1927, 2181
BsiWI CGTACG 1 cut(s) 320
BslFI GGGAC 1 cut(s) 864
BslI CCNNNNNNNGG 1 cut(s) 2347
BsmAI GTCTC 5 cut(s) 249, 664, 1069, 2217, 2282
BsmBI CGTCTC 2 cut(s) 249, 1069
BsmFI GGGAC 1 cut(s) 864
BsmI GAATGC 1 cut(s) 1920
BsnI GGCC 4 cut(s) 96, 1136, 1263, 2271
Bso31I GGTCTC 1 cut(s) 2217
Bsp119I TTCGAA 1 cut(s) 2032
Bsp1286I GDGCHC 2 cut(s) 956, 1283
Bsp13I TCCGGA 2 cut(s) 662, 1886
Bsp143I GATC 9 cut(s) 275, 301, 550, 616, 1034, 1231, 1607, 1950, 2352
Bsp1720I GCTNAGC 1 cut(s) 1660
BspACI CCGC 6 cut(s) 93, 1166, 1319, 1449, 1576, 1824
BspANI GGCC 4 cut(s) 96, 1136, 1263, 2271
BspEI TCCGGA 2 cut(s) 662, 1886
BspHI TCATGA 2 cut(s) 547, 1396
BspLI GGNNCC 4 cut(s) 289, 1036, 2261, 2400
BspPI GGATC 5 cut(s) 283, 296, 1029, 1042, 1602
BspT104I TTCGAA 1 cut(s) 2032
BspTI CTTAAG 1 cut(s) 1675
BspTNI GGTCTC 1 cut(s) 2217
BsrDI GCAATG 2 cut(s) 528, 1187
BsrI ACTGG 3 cut(s) 97, 407, 2312
BssECI CCNNGG 4 cut(s) 132, 375, 1819, 2402
BssMI GATC 9 cut(s) 275, 301, 550, 616, 1034, 1231, 1607, 1950, 2352
BssNAI GTATAC 1 cut(s) 262
BssSI CACGAG 1 cut(s) 1265
BssT1I CCWWGG 3 cut(s) 132, 375, 1819
Bst1107I GTATAC 1 cut(s) 262
Bst2BI CACGAG 1 cut(s) 1265
Bst2UI CCWGG 3 cut(s) 68, 2257, 2403
Bst4CI ACNGT 3 cut(s) 878, 1642, 1993
BstAFI CTTAAG 1 cut(s) 1675
BstAPI GCANNNNNTGC 1 cut(s) 1186
BstBAI YACGTR 2 cut(s) 490, 1226
BstBI TTCGAA 1 cut(s) 2032
BstC8I GCNNGC 2 cut(s) 908, 1187
BstF5I GGATG 3 cut(s) 277, 742, 1651
BstH2I RGCGCY 1 cut(s) 913
BstHHI GCGC 1 cut(s) 912
BstKTI GATC 9 cut(s) 278, 304, 553, 619, 1037, 1234, 1610, 1953, 2355
BstMAI GTCTC 5 cut(s) 249, 664, 1069, 2217, 2282
BstMBI GATC 9 cut(s) 275, 301, 550, 616, 1034, 1231, 1607, 1950, 2352
BstMWI GCNNNNNNNGC 7 cut(s) 77, 143, 1186, 1915, 2035, 2057, 2101
BstNI CCWGG 3 cut(s) 68, 2257, 2403
BstNSI RCATGY 2 cut(s) 47, 1979
BstSCI CCNGG 6 cut(s) 66, 1168, 1407, 2179, 2255, 2401
BstSFI CTRYAG 1 cut(s) 1029
BstSLI GKGCMC 1 cut(s) 956
BstV1I GCAGC 5 cut(s) 200, 794, 816, 1366, 1770
BstV2I GAAGAC 2 cut(s) 119, 1218
BstX2I RGATCY 2 cut(s) 275, 1034
BstXI CCANNNNNNTGG 1 cut(s) 2409
BstYI RGATCY 2 cut(s) 275, 1034
BstZ17I GTATAC 1 cut(s) 262
Bsu36I CCTNAGG 1 cut(s) 1848
BsuI GTATCC 1 cut(s) 2320
BsuRI GGCC 4 cut(s) 96, 1136, 1263, 2271
BtgZI GCGATG 1 cut(s) 450
BtsCI GGATG 3 cut(s) 277, 742, 1651
BtsIMutI CAGTG 4 cut(s) 237, 616, 1076, 1638
Cac8I GCNNGC 2 cut(s) 908, 1187
CaiI CAGNNNCTG 1 cut(s) 1693
CciI TCATGA 2 cut(s) 547, 1396
CfoI GCGC 1 cut(s) 912
Cfr13I GGNCC 4 cut(s) 25, 771, 1405, 1816
CseI GACGC 1 cut(s) 2066
Csp6I GTAC 8 cut(s) 233, 321, 479, 704, 1964, 1986, 2284, 2336
CspCI CAANNNNNGTGG 2 cut(s) 1978, 2013
CviAII CATG 9 cut(s) 44, 548, 733, 1097, 1397, 1920, 1976, 2347, 2385
CviQI GTAC 8 cut(s) 233, 321, 479, 704, 1964, 1986, 2284, 2336
DpnI GATC 9 cut(s) 277, 303, 552, 618, 1036, 1233, 1609, 1952, 2354
DpnII GATC 9 cut(s) 275, 301, 550, 616, 1034, 1231, 1607, 1950, 2352
EaeI YGGCCR 1 cut(s) 94
Eco130I CCWWGG 3 cut(s) 132, 375, 1819
Eco147I AGGCCT 2 cut(s) 1263, 2271
Eco24I GRGCYC 1 cut(s) 1283
Eco31I GGTCTC 1 cut(s) 2217
Eco47I GGWCC 4 cut(s) 25, 771, 1405, 1816
Eco57I CTGAAG 2 cut(s) 73, 1196
Eco81I CCTNAGG 1 cut(s) 1848
EcoRI GAATTC 2 cut(s) 693, 1597
EcoRII CCWGG 3 cut(s) 66, 2255, 2401
EcoT14I CCWWGG 3 cut(s) 132, 375, 1819
EcoT38I GRGCYC 1 cut(s) 1283
ErhI CCWWGG 3 cut(s) 132, 375, 1819
Esp3I CGTCTC 2 cut(s) 249, 1069
FaeI CATG 9 cut(s) 47, 551, 736, 1100, 1400, 1923, 1979, 2350, 2388
FalI AAGNNNNNCTT 6 cut(s) 951, 983, 1585, 1617, 1852, 1884
FaqI GGGAC 1 cut(s) 864
FatI CATG 9 cut(s) 43, 547, 732, 1096, 1396, 1919, 1975, 2346, 2384
FauI CCCGC 2 cut(s) 1173, 1817
FbaI TGATCA 2 cut(s) 1231, 1950
FblI GTMKAC 5 cut(s) 261, 564, 1056, 1380, 2304
Fnu4HI GCNGC 8 cut(s) 94, 189, 805, 808, 1355, 1450, 1576, 1759
FokI GGATG 3 cut(s) 284, 749, 1658
FriOI GRGCYC 1 cut(s) 1283
Fsp4HI GCNGC 8 cut(s) 94, 189, 805, 808, 1355, 1450, 1576, 1759
GlaI GCGC 1 cut(s) 911
GluI GCNGC 8 cut(s) 94, 189, 805, 808, 1355, 1450, 1576, 1759
GsaI CCCAGC 1 cut(s) 2173
GsuI CTGGAG 1 cut(s) 1106
HaeII RGCGCY 1 cut(s) 913
HaeIII GGCC 4 cut(s) 96, 1136, 1263, 2271
HapII CCGG 7 cut(s) 285, 663, 1170, 1408, 1887, 1927, 2181
HgaI GACGC 1 cut(s) 2066
HhaI GCGC 1 cut(s) 912
Hin1II CATG 9 cut(s) 47, 551, 736, 1100, 1400, 1923, 1979, 2350, 2388
Hin6I GCGC 1 cut(s) 910
HinP1I GCGC 1 cut(s) 910
HincII GTYRAC 1 cut(s) 1057
HindII GTYRAC 1 cut(s) 1057
HpaII CCGG 7 cut(s) 285, 663, 1170, 1408, 1887, 1927, 2181
HphI GGTGA 2 cut(s) 121, 397
Hpy166II GTNNAC 8 cut(s) 51, 262, 565, 771, 1007, 1057, 1381, 2305
Hpy188I TCNGA 5 cut(s) 621, 681, 1176, 1616, 1695
Hpy8I GTNNAC 8 cut(s) 51, 262, 565, 771, 1007, 1057, 1381, 2305
Hpy99I CGWCG 1 cut(s) 2060
HpyCH4III ACNGT 3 cut(s) 878, 1642, 1993
HpyCH4IV ACGT 4 cut(s) 258, 489, 1225, 1537
HpyF10VI GCNNNNNNNGC 7 cut(s) 77, 143, 1186, 1915, 2035, 2057, 2101
HpySE526I ACGT 4 cut(s) 258, 489, 1225, 1537
Hsp92II CATG 9 cut(s) 47, 551, 736, 1100, 1400, 1923, 1979, 2350, 2388
HspAI GCGC 1 cut(s) 910
Kpn2I TCCGGA 2 cut(s) 662, 1886
Ksp22I TGATCA 2 cut(s) 1231, 1950
Kzo9I GATC 9 cut(s) 275, 301, 550, 616, 1034, 1231, 1607, 1950, 2352
LmnI GCTCC 4 cut(s) 40, 293, 630, 665
Lsp1109I GCAGC 5 cut(s) 200, 794, 816, 1366, 1770
LweI GCATC 6 cut(s) 325, 819, 1055, 1325, 1673, 2220
MaeII ACGT 4 cut(s) 258, 489, 1225, 1537
MaeIII GTNAC 5 cut(s) 127, 138, 673, 817, 2363
MalI GATC 9 cut(s) 277, 303, 552, 618, 1036, 1233, 1609, 1952, 2354
MboI GATC 9 cut(s) 275, 301, 550, 616, 1034, 1231, 1607, 1950, 2352
MflI RGATCY 2 cut(s) 275, 1034
MhlI GDGCHC 2 cut(s) 956, 1283
MlyI GAGTC 7 cut(s) 25, 166, 880, 1002, 1052, 1386, 1912
MmeI TCCRAC 4 cut(s) 408, 434, 1602, 1759
MroI TCCGGA 2 cut(s) 662, 1886
MroXI GAANNNNTTC 2 cut(s) 1864, 2378
MseI TTAA 7 cut(s) 8, 450, 891, 1238, 1676, 2175, 2422
MspA1I CMGCKG 2 cut(s) 93, 807
MspCI CTTAAG 1 cut(s) 1675
MspI CCGG 7 cut(s) 285, 663, 1170, 1408, 1887, 1927, 2181
MspR9I CCNGG 6 cut(s) 68, 1170, 1409, 2181, 2257, 2403
Mva1269I GAATGC 1 cut(s) 1920
MvaI CCWGG 3 cut(s) 68, 2257, 2403
MwoI GCNNNNNNNGC 7 cut(s) 77, 143, 1186, 1915, 2035, 2057, 2101
NciI CCSGG 3 cut(s) 1170, 1409, 2181
NdeII GATC 9 cut(s) 275, 301, 550, 616, 1034, 1231, 1607, 1950, 2352
NlaIII CATG 9 cut(s) 47, 551, 736, 1100, 1400, 1923, 1979, 2350, 2388
NlaIV GGNNCC 4 cut(s) 289, 1036, 2261, 2400
NmuCI GTSAC 1 cut(s) 127
NspI RCATGY 2 cut(s) 47, 1979
NspV TTCGAA 1 cut(s) 2032
PagI TCATGA 2 cut(s) 547, 1396
PceI AGGCCT 2 cut(s) 1263, 2271
PciI ACATGT 1 cut(s) 1975
PcsI WCGNNNNNNNCGW 1 cut(s) 874
PctI GAATGC 1 cut(s) 1920
PdmI GAANNNNTTC 2 cut(s) 1864, 2378
PfeI GAWTC 5 cut(s) 406, 748, 870, 1393, 1890
Pfl23II CGTACG 1 cut(s) 320
PflFI GACNNNGTC 1 cut(s) 2228
PflMI CCANNNNNTGG 1 cut(s) 2347
PfoI TCCNGGA 3 cut(s) 1407, 2179, 2255
PkrI GCNGC 8 cut(s) 95, 190, 806, 809, 1356, 1451, 1577, 1760
PleI GAGTC 7 cut(s) 25, 165, 880, 1002, 1052, 1385, 1911
PpsI GAGTC 7 cut(s) 25, 165, 880, 1002, 1052, 1385, 1911
Ppu21I YACGTR 2 cut(s) 490, 1226
PscI ACATGT 1 cut(s) 1975
Psp6I CCWGG 3 cut(s) 66, 2255, 2401
PspFI CCCAGC 1 cut(s) 2169
PspGI CCWGG 3 cut(s) 66, 2255, 2401
PspLI CGTACG 1 cut(s) 320
PspN4I GGNNCC 4 cut(s) 289, 1036, 2261, 2400
PspPI GGNCC 4 cut(s) 25, 771, 1405, 1816
PstNI CAGNNNCTG 1 cut(s) 1693
PsuI RGATCY 2 cut(s) 275, 1034
PsyI GACNNNGTC 1 cut(s) 2228
PvuII CAGCTG 1 cut(s) 807
RsaI GTAC 8 cut(s) 234, 322, 480, 705, 1965, 1987, 2285, 2337
RsaNI GTAC 8 cut(s) 233, 321, 479, 704, 1964, 1986, 2284, 2336
SalI GTCGAC 1 cut(s) 1055
SaqAI TTAA 7 cut(s) 8, 450, 891, 1238, 1676, 2175, 2422
SatI GCNGC 8 cut(s) 94, 189, 805, 808, 1355, 1450, 1576, 1759
Sau3AI GATC 9 cut(s) 275, 301, 550, 616, 1034, 1231, 1607, 1950, 2352
Sau96I GGNCC 4 cut(s) 25, 771, 1405, 1816
ScaI AGTACT 3 cut(s) 705, 1965, 2285
SchI GAGTC 7 cut(s) 25, 166, 880, 1002, 1052, 1386, 1912
ScrFI CCNGG 6 cut(s) 68, 1170, 1409, 2181, 2257, 2403
SduI GDGCHC 2 cut(s) 956, 1283
SfaNI GCATC 6 cut(s) 325, 819, 1055, 1325, 1673, 2220
SfcI CTRYAG 1 cut(s) 1029
SfuI TTCGAA 1 cut(s) 2032
SinI GGWCC 4 cut(s) 25, 771, 1405, 1816
SmlI CTYRAG 5 cut(s) 968, 1282, 1675, 1934, 2279
SmoI CTYRAG 5 cut(s) 968, 1282, 1675, 1934, 2279
SseBI AGGCCT 2 cut(s) 1263, 2271
SsiI CCGC 6 cut(s) 93, 1166, 1319, 1449, 1576, 1824
StuI AGGCCT 2 cut(s) 1263, 2271
StyD4I CCNGG 6 cut(s) 66, 1168, 1407, 2179, 2255, 2401
StyI CCWWGG 3 cut(s) 132, 375, 1819
TaaI ACNGT 3 cut(s) 878, 1642, 1993
TaiI ACGT 4 cut(s) 261, 492, 1228, 1540
TaqI TCGA 5 cut(s) 465, 1056, 2032, 2055, 2214
TatI WGTACW 5 cut(s) 232, 703, 1963, 2283, 2335
TauI GCSGC 3 cut(s) 96, 1452, 1578
TfiI GAWTC 5 cut(s) 406, 748, 870, 1393, 1890
Tru1I TTAA 7 cut(s) 8, 450, 891, 1238, 1676, 2175, 2422
Tru9I TTAA 7 cut(s) 8, 450, 891, 1238, 1676, 2175, 2422
TscAI CASTG 4 cut(s) 244, 616, 1076, 1645
TseFI GTSAC 1 cut(s) 127
TseI GCWGC 5 cut(s) 188, 804, 807, 1354, 1758
Tsp45I GTSAC 1 cut(s) 127
TspGWI ACGGA 2 cut(s) 53, 167
TspRI CASTG 4 cut(s) 244, 616, 1076, 1645
Tth111I GACNNNGTC 1 cut(s) 2228
Van91I CCANNNNNTGG 1 cut(s) 2347
Vha464I CTTAAG 1 cut(s) 1675
VpaK11BI GGWCC 4 cut(s) 25, 771, 1405, 1816
XapI RAATTY 3 cut(s) 74, 693, 1597
XbaI TCTAGA 2 cut(s) 553, 1765
XceI RCATGY 2 cut(s) 47, 1979
XcmI CCANNNNNNNNNTGG 1 cut(s) 1250
XmaJI CCTAGG 1 cut(s) 1819
XmiI GTMKAC 5 cut(s) 261, 564, 1056, 1380, 2304
XmnI GAANNNNTTC 2 cut(s) 1864, 2378
ZrmI AGTACT 3 cut(s) 705, 1965, 2285
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.