RchiOBHm_Chr4g0391451

NB-ARC domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0000540
Physical Location & Seq
Forward (+)
6886575 .. 6888857
2283 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ36427

Sequence Viewer

Length: 2157 bp
ATGAAGGAGATAGAGCAGACCTTGGGCAAGTTTTTGAGGGCTCCTGATAACATCCATAGAGAAGATGCATACCAAGTTAGGGTGATGGTTTCACAGGACTGGAATCCAACTGAGATAATTGGGTTGGATAGCGATGTGGAGAAGATTAAGGGTTGGCTTTTCGACACTAGCAAGGTACTTTCCCACGTAGGCATTGTGGGAATGGGAGGCTTGGGGAAAACCACCATTGCTCAAAAGATATTTCATGATCTAGAAGTAGTAGGCCACTTTCACAAGCTGATATGGGTGTGTGTTTCGCAATCATTCAGTGCTGATCGGATTATGAGGAGCATCCTAGAAGGTCTAGGAGAAAATGCCTCCGGAGCTGGTGTTACTCAGATTTTGAGCAGAATTCAGCAAGTACTTAAAGATAAGAGCTGTCTGATTGTCATGGATGATGTGTGGAATCAAAAAGATGTTGATTGGTGGACCAACCTCTACTCTGTTATACCCAAACAAAGCAGCTGCATCATCATTACAACTAGAAATGAAGAAGCTGTAATTGGTATGGGAGTTGAAAGCTCACGAATCCACCGTCCCAAGACTCTTAATGATGAGGAAAGCTGGCGCTTGTTCTCCAAGTTTGCATTTCCTGCAAGCAAGGGATTGTGCCCGGAGGAAAGGCTTGAGAAATTAGGAAAAGAACTCTTGAAGAAGTGTGGTGGACTCCCACTCGCAATCAAGACTATAGGATCCTTGTTGGCATCAAAGGTCGACTCCCCTTCTCAGTGGAGACGAGTTCTGGAGAGCTTTCATGCTTTGACAACAGAGGGAAAAACTAGCTCAGTTATGGCCTCTTTGCGATTGAGCTATGATGAACTCCCGCCTGGTCTGAAGCAATGCCTGCTGTGTTTCTCCATCTATCCCGAAGACTATGAGATACGTGCTGATCAGTTAATCCATTGGTGGATTGGAGAAGGCCTCGTGCAAAGCAAGGGCTCAAGAACAGCAGTAGAAGTGGGATATGAGTATCTTGCGGAACTGATAAGCAGATGCTTGGTTGAAATTGTGCAGCAGAGAGGTTTTGATGGGAGAGTCTACAAGTGCAAGATTCATGATATGGTCCGGGAACTGATAATAATGATTGCAGAGGAGGAGGAATTATGCTGCTTTGATGAACAAGGCAGACAGAAATTGACAGCAAATACTAGGTGGTTATGCTTTATTGATGAAATGGAGGAGAAGTCCATGAAAAGTAGTTCAAAGCTCAGGGCATTGTTGATGATGTCAAGCCGCAGATTTGACTTTGAAAGGAATTCTTTGCGATCCCTCAGAATGTTGGATTTATCAAACTGCACTGTGGATGAGCATTGTGTGAAGGATCTCTTCAGCTGGATCAGCTCCCTTAAGCGCTTAGCATCTTTGAACTTAAGTGGGATTCAGGCTCTGAAAGAACTGCCATCTTCAATTCATAAACTCCTCAACCTCCAACTTTTACTTCTAACAGGTTGCAGCAATCTAGAAAAAATACACCCTTCCATCACTAATTTGAAGAAGCTGATTATTTTGGACCTAGGCGGGTGTCCAATCCAATACTTACCTCAGGGATTGGGAAGGCTTTCTTATCTTCAAGAGTTCTCCGGATTCAAGATTGTGAGTCAGCCTAGAATGCCATGTTTCCGGCTTCTTGAGATTAGGGAACTGATCAACCTGAGAGTACTGCGAATACATGTAAGCAGTACCACAGTTATTGCAGACAATGAACTTGATGTCCTCTCTCAGCTTCGAAGGCTCAATGTTCTGGCTATCGACGCAGAAGATTGTAGAAATAACAATGTTTTGGAAATGCTAGAAAGGCTGACCCCTCCTCCAAGCCTCCAAGAGCTGTATCTTAGGAATTACAAGAAGGAAACTCTCCCTAGCTGGGTTAATCCCGGACAGCTTTCAAGATTGCAGTATCTTTGCATCGAGAATGGAGACCTTGTCAAGCTAAGTAGTGGTCAAACCACCTGGAACCTTGAAGGCCTGTGTCTCAAGTACTTGATGAGGCTAGAGGTAGACTGGAAGGACTTGGAGAAGGATATGCCTGTACTCCACTACATGGAGATCAGTCATTGTTACAACTTGAAGGATTTCCCATGTTCAGTTATGGAACCAGGGGTCTGGAGAAAGAATTAA

Protein Analysis

718

Amino Acids

82.08

Weight (kDa)

6.75

Isoelectric Point (pI)

46.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 44 - 213 1.2e-40 NB-ARC domain
AAA_22 PF13401 65 - 147 1.8e-06 AAA domain
WHD_DRP PF23559 300 - 372 1.8e-20 Disease resistance protein Winged helix domain
LRR_14 PF23598 429 - 650 9.8e-28 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 570 - 659 1.2e-08 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000540)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G50950 AT3G50950
fragaria_vesca FvH4_1g12710 FvH4_1g12710 FvH4_3g00820 FvH4_3g00820 FvH4_3g00820 FvH4_3g00820
malus_domestica MD02G1141100.v1.1 MD10G1333100.v1.1
prunus_persica Prupe.4G009300_v2.0.a1 Prupe.4G009300_v2.0.a1 Prupe.4G009300_v2.0.a1 Prupe.6G150700_v2.0.a1 Prupe.7G160100_v2.0.a1 Prupe.7G160100_v2.0.a1
pyrus_communis pycom02g11110 pycom10g28240 pycom10g28340 pycom10g28360 pycom10g28450
rosa_chinensis RchiOBHm_Chr2g0100711 RchiOBHm_Chr3g0485861 RchiOBHm_Chr4g0391451 RchiOBHm_Chr4g0391591 RchiOBHm_Chr4g0391601 RchiOBHm_Chr4g0391681 RchiOBHm_Chr5g0001141 RchiOBHm_Chr5g0001221
rosa_laevigata RLG00000017001
rosa_multiflora Rmu_co8260007.1_g000001 Rmu_co8260103.1_g000001 Rmu_co8519155.1_g000001 Rmu_sc0000060.1_g000050 Rmu_sc0000255.1_g000036 Rmu_sc0001174.1_g000023 Rmu_sc0001174.1_g000025 Rmu_sc0001659.1_g000009 Rmu_sc0001706.1_g000009 Rmu_sc0001706.1_g000024 Rmu_sc0001706.1_g000035 Rmu_sc0004189.1_g000018 Rmu_sc0004189.1_g000030 Rmu_sc0004862.1_g000002 Rmu_sc0004876.1_g000038 Rmu_sc0023741.1_g000004
rosa_roxburghii Rroxscaffold_1G00075100 Rroxscaffold_1G00075160 Rroxscaffold_1G00075210 Rroxscaffold_5G00337160 Rroxscaffold_5G00386590 Rroxscaffold_6G00396300
rosa_rugosa Rorug03G0336200 Rorug03G0336300 Rorug04G0389400
rosa_samantha Rh2AG140400 Rh2BG144100 Rh2CG145400 Rh2DG144900 Rh3AG271200 Rh4AG038000 Rh4AG038700 Rh4BG032000 Rh4BG032800 Rh4CG041100 Rh4CG041300 Rh4DG035500 Rh4DG035600 Rh5AG008800 Rh5AG009100 Rh5BG010900 Rh5BG011500 Rh5BG011600 Rh5BG012000 Rh5CG009400 Rh5CG010000 Rh5DG009800
rosa_wichuraiana Rw2G010940 Rw4G002950 Rw4G002990 Rw4G003000 Rw5G000880 Rw5G000950 Rw5G000960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 2080
AccI GTMKAC 3 cut(s) 753, 1077, 2037
AccIII TCCGGA 2 cut(s) 359, 1619
AciI CCGC 4 cut(s) 863, 1016, 1273, 1557
AclWI GGATC 5 cut(s) 726, 739, 1299, 1368, 1382
AcsI RAATTY 2 cut(s) 390, 1294
AcuI CTGAAG 2 cut(s) 893, 1351
AfaI GTAC 6 cut(s) 177, 402, 1698, 1720, 2018, 2070
AfeI AGCGCT 1 cut(s) 1391
AfiI CCNNNNNNNGG 3 cut(s) 79, 1903, 2080
AflII CTTAAG 2 cut(s) 1385, 1408
AflIII ACRYGT 1 cut(s) 1708
AjnI CCWGG 3 cut(s) 865, 1988, 2134
AjuI GAANNNNNNNTTGG 4 cut(s) 525, 557, 1461, 1493
AloI GAACNNNNNNTCC 2 cut(s) 1734, 1766
Alw26I GTCTC 3 cut(s) 766, 1950, 2015
AlwI GGATC 5 cut(s) 726, 739, 1299, 1368, 1382
AlwNI CAGNNNCTG 1 cut(s) 1426
Aor13HI TCCGGA 2 cut(s) 359, 1619
Aor51HI AGCGCT 1 cut(s) 1391
AoxI GGCC 4 cut(s) 262, 831, 958, 2002
ApeKI GCWGC 5 cut(s) 501, 504, 1051, 1146, 1491
ApoI RAATTY 2 cut(s) 390, 1294
ArsI GACNNNNNNTTYG 2 cut(s) 559, 591
Asp700I GAANNNNTTC 2 cut(s) 1597, 2111
AspA2I CCTAGG 1 cut(s) 1552
AspLEI GCGC 2 cut(s) 609, 1392
AspS9I GGNCC 3 cut(s) 468, 1102, 1549
AsuC2I CCSGG 3 cut(s) 653, 1106, 1914
AsuHPI GGTGA 1 cut(s) 94
AsuII TTCGAA 1 cut(s) 1765
AvaII GGWCC 3 cut(s) 468, 1102, 1549
AvrII CCTAGG 1 cut(s) 1552
AxyI CCTNAGG 1 cut(s) 1581
BaeGI GKGCMC 1 cut(s) 653
BamHI GGATCC 1 cut(s) 731
BanII GRGCYC 2 cut(s) 43, 980
BauI CACGAG 1 cut(s) 962
BbsI GAAGAC 1 cut(s) 915
BbvI GCAGC 5 cut(s) 491, 513, 1063, 1133, 1503
BccI CCATC 5 cut(s) 79, 905, 1061, 1447, 1526
BciT130I CCWGG 3 cut(s) 867, 1990, 2136
BclI TGATCA 2 cut(s) 928, 1683
BcnI CCSGG 3 cut(s) 653, 1106, 1914
BcoDI GTCTC 3 cut(s) 766, 1950, 2015
BfmI CTRYAG 1 cut(s) 726
BfoI RGCGCY 2 cut(s) 610, 1393
BfrI CTTAAG 2 cut(s) 1385, 1408
BisI GCNGC 6 cut(s) 502, 505, 1052, 1147, 1273, 1492
BlnI CCTAGG 1 cut(s) 1552
BlpI GCTNAGC 1 cut(s) 1393
BlsI GCNGC 6 cut(s) 503, 506, 1053, 1148, 1274, 1493
BmcAI AGTACT 3 cut(s) 402, 1698, 2018
Bme1390I CCNGG 6 cut(s) 653, 867, 1106, 1914, 1990, 2136
Bme18I GGWCC 3 cut(s) 468, 1102, 1549
BmgT120I GGNCC 3 cut(s) 468, 1102, 1549
BmiI GGNNCC 4 cut(s) 42, 733, 1994, 2133
BmrFI CCNGG 6 cut(s) 653, 867, 1106, 1914, 1990, 2136
BmsI GCATC 7 cut(s) 55, 339, 516, 752, 1022, 1406, 1953
BpiI GAAGAC 1 cut(s) 915
BplI GAGNNNNNCTC 2 cut(s) 945, 977
BpmI CTGGAG 1 cut(s) 803
Bpu10I CCTNAGC 1 cut(s) 1247
Bpu1102I GCTNAGC 1 cut(s) 1393
Bpu14I TTCGAA 1 cut(s) 1765
BpuEI CTTGAG 4 cut(s) 686, 964, 1688, 1997
BpuMI CCSGG 3 cut(s) 653, 1106, 1914
BsaAI YACGTR 2 cut(s) 187, 923
BsaI GGTCTC 1 cut(s) 1950
BsaJI CCNNGG 3 cut(s) 21, 1552, 2135
BsaWI WCCGGW 2 cut(s) 359, 1619
BsaXI ACNNNNNCTCC 8 cut(s) 354, 384, 1208, 1238, 1831, 1861, 1947, 1977
Bsc4I CCNNNNNNNGG 3 cut(s) 79, 1903, 2080
Bse1I ACTGG 2 cut(s) 104, 2045
Bse21I CCTNAGG 1 cut(s) 1581
Bse3DI GCAATG 2 cut(s) 225, 884
BseAI TCCGGA 2 cut(s) 359, 1619
BseBI CCWGG 3 cut(s) 867, 1990, 2136
BseDI CCNNGG 3 cut(s) 21, 1552, 2135
BseGI GGATG 4 cut(s) 51, 330, 439, 1348
BseLI CCNNNNNNNGG 3 cut(s) 79, 1903, 2080
BseMI GCAATG 2 cut(s) 225, 884
BseMII CTCAG 9 cut(s) 102, 389, 779, 837, 1261, 1324, 1595, 1682, 1772
BseNI ACTGG 2 cut(s) 104, 2045
BseRI GAGGAG 6 cut(s) 340, 1145, 1148, 1232, 1448, 1836
BseSI GKGCMC 1 cut(s) 653
BseXI GCAGC 5 cut(s) 491, 513, 1063, 1133, 1503
BseYI CCCAGC 1 cut(s) 1902
BsgI GTGCAG 2 cut(s) 1070, 1318
BshFI GGCC 4 cut(s) 264, 833, 960, 2004
BsiSI CCGG 6 cut(s) 360, 653, 1105, 1620, 1660, 1914
BslFI GGGAC 1 cut(s) 561
BslI CCNNNNNNNGG 3 cut(s) 79, 1903, 2080
BsmAI GTCTC 3 cut(s) 766, 1950, 2015
BsmBI CGTCTC 1 cut(s) 766
BsmFI GGGAC 1 cut(s) 561
BsmI GAATGC 1 cut(s) 1653
BsnI GGCC 4 cut(s) 264, 833, 960, 2004
Bso31I GGTCTC 1 cut(s) 1950
Bsp119I TTCGAA 1 cut(s) 1765
Bsp1286I GDGCHC 3 cut(s) 43, 653, 980
Bsp13I TCCGGA 2 cut(s) 359, 1619
Bsp143I GATC 9 cut(s) 247, 313, 731, 928, 1304, 1360, 1374, 1683, 2085
Bsp1720I GCTNAGC 1 cut(s) 1393
BspACI CCGC 4 cut(s) 863, 1016, 1273, 1557
BspANI GGCC 4 cut(s) 264, 833, 960, 2004
BspCNI CTCAG 9 cut(s) 103, 388, 778, 836, 1260, 1323, 1594, 1683, 1771
BspEI TCCGGA 2 cut(s) 359, 1619
BspHI TCATGA 2 cut(s) 244, 1093
BspLI GGNNCC 4 cut(s) 42, 733, 1994, 2133
BspPI GGATC 5 cut(s) 726, 739, 1299, 1368, 1382
BspT104I TTCGAA 1 cut(s) 1765
BspTI CTTAAG 2 cut(s) 1385, 1408
BspTNI GGTCTC 1 cut(s) 1950
BsrDI GCAATG 2 cut(s) 225, 884
BsrI ACTGG 2 cut(s) 104, 2045
BssECI CCNNGG 3 cut(s) 21, 1552, 2135
BssMI GATC 9 cut(s) 247, 313, 731, 928, 1304, 1360, 1374, 1683, 2085
BssSI CACGAG 1 cut(s) 962
BssT1I CCWWGG 2 cut(s) 21, 1552
Bst2BI CACGAG 1 cut(s) 962
Bst2UI CCWGG 3 cut(s) 867, 1990, 2136
Bst4CI ACNGT 3 cut(s) 575, 1339, 1726
Bst6I CTCTTC 1 cut(s) 1370
BstAFI CTTAAG 2 cut(s) 1385, 1408
BstAPI GCANNNNNTGC 2 cut(s) 632, 883
BstBAI YACGTR 2 cut(s) 187, 923
BstBI TTCGAA 1 cut(s) 1765
BstC8I GCNNGC 3 cut(s) 605, 637, 884
BstF5I GGATG 4 cut(s) 51, 330, 439, 1348
BstH2I RGCGCY 2 cut(s) 610, 1393
BstHHI GCGC 2 cut(s) 609, 1392
BstKTI GATC 9 cut(s) 250, 316, 734, 931, 1307, 1363, 1377, 1686, 2088
BstMAI GTCTC 3 cut(s) 766, 1950, 2015
BstMBI GATC 9 cut(s) 247, 313, 731, 928, 1304, 1360, 1374, 1683, 2085
BstMWI GCNNNNNNNGC 8 cut(s) 362, 632, 883, 1377, 1648, 1768, 1790, 1834
BstNI CCWGG 3 cut(s) 867, 1990, 2136
BstNSI RCATGY 1 cut(s) 1712
BstSCI CCNGG 6 cut(s) 651, 865, 1104, 1912, 1988, 2134
BstSFI CTRYAG 1 cut(s) 726
BstSLI GKGCMC 1 cut(s) 653
BstV1I GCAGC 5 cut(s) 491, 513, 1063, 1133, 1503
BstV2I GAAGAC 1 cut(s) 915
BstX2I RGATCY 2 cut(s) 731, 1360
BstXI CCANNNNNNTGG 1 cut(s) 2142
BstYI RGATCY 2 cut(s) 731, 1360
Bsu36I CCTNAGG 1 cut(s) 1581
BsuRI GGCC 4 cut(s) 264, 833, 960, 2004
BtgZI GCGATG 1 cut(s) 147
BtsCI GGATG 4 cut(s) 51, 330, 439, 1348
BtsIMutI CAGTG 3 cut(s) 313, 773, 1335
Cac8I GCNNGC 3 cut(s) 605, 637, 884
CaiI CAGNNNCTG 1 cut(s) 1426
CciI TCATGA 2 cut(s) 244, 1093
CfoI GCGC 2 cut(s) 609, 1392
Cfr13I GGNCC 3 cut(s) 468, 1102, 1549
CseI GACGC 1 cut(s) 1799
Csp6I GTAC 6 cut(s) 176, 401, 1697, 1719, 2017, 2069
CspCI CAANNNNNGTGG 2 cut(s) 1711, 1746
CviAII CATG 9 cut(s) 245, 430, 794, 1094, 1228, 1653, 1709, 2080, 2118
CviQI GTAC 6 cut(s) 176, 401, 1697, 1719, 2017, 2069
DpnI GATC 9 cut(s) 249, 315, 733, 930, 1306, 1362, 1376, 1685, 2087
DpnII GATC 9 cut(s) 247, 313, 731, 928, 1304, 1360, 1374, 1683, 2085
Eam1104I CTCTTC 1 cut(s) 1370
EarI CTCTTC 1 cut(s) 1370
Eco130I CCWWGG 2 cut(s) 21, 1552
Eco147I AGGCCT 2 cut(s) 960, 2004
Eco24I GRGCYC 2 cut(s) 43, 980
Eco31I GGTCTC 1 cut(s) 1950
Eco47I GGWCC 3 cut(s) 468, 1102, 1549
Eco47III AGCGCT 1 cut(s) 1391
Eco57I CTGAAG 2 cut(s) 893, 1351
Eco81I CCTNAGG 1 cut(s) 1581
EcoRI GAATTC 2 cut(s) 390, 1294
EcoRII CCWGG 3 cut(s) 865, 1988, 2134
EcoT14I CCWWGG 2 cut(s) 21, 1552
EcoT22I ATGCAT 1 cut(s) 70
EcoT38I GRGCYC 2 cut(s) 43, 980
ErhI CCWWGG 2 cut(s) 21, 1552
Esp3I CGTCTC 1 cut(s) 766
FaeI CATG 9 cut(s) 248, 433, 797, 1097, 1231, 1656, 1712, 2083, 2121
FalI AAGNNNNNCTT 6 cut(s) 1282, 1314, 1349, 1381, 1585, 1617
FaqI GGGAC 1 cut(s) 561
FatI CATG 9 cut(s) 244, 429, 793, 1093, 1227, 1652, 1708, 2079, 2117
FauI CCCGC 2 cut(s) 870, 1550
FbaI TGATCA 2 cut(s) 928, 1683
FblI GTMKAC 3 cut(s) 753, 1077, 2037
Fnu4HI GCNGC 6 cut(s) 502, 505, 1052, 1147, 1273, 1492
FokI GGATG 4 cut(s) 38, 317, 446, 1355
FriOI GRGCYC 2 cut(s) 43, 980
Fsp4HI GCNGC 6 cut(s) 502, 505, 1052, 1147, 1273, 1492
GlaI GCGC 2 cut(s) 608, 1391
GluI GCNGC 6 cut(s) 502, 505, 1052, 1147, 1273, 1492
GsaI CCCAGC 1 cut(s) 1906
GsuI CTGGAG 1 cut(s) 803
HaeII RGCGCY 2 cut(s) 610, 1393
HaeIII GGCC 4 cut(s) 264, 833, 960, 2004
HapII CCGG 6 cut(s) 360, 653, 1105, 1620, 1660, 1914
HgaI GACGC 1 cut(s) 1799
HhaI GCGC 2 cut(s) 609, 1392
Hin1II CATG 9 cut(s) 248, 433, 797, 1097, 1231, 1656, 1712, 2083, 2121
Hin6I GCGC 2 cut(s) 607, 1390
HinP1I GCGC 2 cut(s) 607, 1390
HincII GTYRAC 1 cut(s) 754
HindII GTYRAC 1 cut(s) 754
HpaII CCGG 6 cut(s) 360, 653, 1105, 1620, 1660, 1914
HphI GGTGA 1 cut(s) 94
Hpy166II GTNNAC 5 cut(s) 468, 704, 754, 1078, 2038
Hpy188I TCNGA 6 cut(s) 318, 378, 423, 873, 1313, 1428
Hpy8I GTNNAC 5 cut(s) 468, 704, 754, 1078, 2038
Hpy99I CGWCG 1 cut(s) 1793
HpyCH4III ACNGT 3 cut(s) 575, 1339, 1726
HpyCH4IV ACGT 2 cut(s) 186, 922
HpyF10VI GCNNNNNNNGC 8 cut(s) 362, 632, 883, 1377, 1648, 1768, 1790, 1834
HpySE526I ACGT 2 cut(s) 186, 922
Hsp92II CATG 9 cut(s) 248, 433, 797, 1097, 1231, 1656, 1712, 2083, 2121
HspAI GCGC 2 cut(s) 607, 1390
Kpn2I TCCGGA 2 cut(s) 359, 1619
Ksp22I TGATCA 2 cut(s) 928, 1683
Kzo9I GATC 9 cut(s) 247, 313, 731, 928, 1304, 1360, 1374, 1683, 2085
LmnI GCTCC 4 cut(s) 46, 327, 362, 1385
Lsp1109I GCAGC 5 cut(s) 491, 513, 1063, 1133, 1503
LweI GCATC 7 cut(s) 55, 339, 516, 752, 1022, 1406, 1953
MaeII ACGT 2 cut(s) 186, 922
MaeIII GTNAC 2 cut(s) 370, 2096
MalI GATC 9 cut(s) 249, 315, 733, 930, 1306, 1362, 1376, 1685, 2087
MboI GATC 9 cut(s) 247, 313, 731, 928, 1304, 1360, 1374, 1683, 2085
MflI RGATCY 2 cut(s) 731, 1360
MhlI GDGCHC 3 cut(s) 43, 653, 980
MlyI GAGTC 5 cut(s) 577, 699, 749, 1083, 1645
MmeI TCCRAC 4 cut(s) 105, 131, 1299, 1492
Mph1103I ATGCAT 1 cut(s) 70
MroI TCCGGA 2 cut(s) 359, 1619
MroXI GAANNNNTTC 2 cut(s) 1597, 2111
MseI TTAA 8 cut(s) 147, 405, 588, 935, 1386, 1409, 1908, 2155
MspA1I CMGCKG 2 cut(s) 504, 1371
MspCI CTTAAG 2 cut(s) 1385, 1408
MspI CCGG 6 cut(s) 360, 653, 1105, 1620, 1660, 1914
MspR9I CCNGG 6 cut(s) 653, 867, 1106, 1914, 1990, 2136
Mva1269I GAATGC 1 cut(s) 1653
MvaI CCWGG 3 cut(s) 867, 1990, 2136
MwoI GCNNNNNNNGC 8 cut(s) 362, 632, 883, 1377, 1648, 1768, 1790, 1834
NciI CCSGG 3 cut(s) 653, 1106, 1914
NdeII GATC 9 cut(s) 247, 313, 731, 928, 1304, 1360, 1374, 1683, 2085
NlaIII CATG 9 cut(s) 248, 433, 797, 1097, 1231, 1656, 1712, 2083, 2121
NlaIV GGNNCC 4 cut(s) 42, 733, 1994, 2133
NsiI ATGCAT 1 cut(s) 70
NspI RCATGY 1 cut(s) 1712
NspV TTCGAA 1 cut(s) 1765
PagI TCATGA 2 cut(s) 244, 1093
PceI AGGCCT 2 cut(s) 960, 2004
PciI ACATGT 1 cut(s) 1708
PcsI WCGNNNNNNNCGW 1 cut(s) 571
PctI GAATGC 1 cut(s) 1653
PdmI GAANNNNTTC 2 cut(s) 1597, 2111
PfeI GAWTC 6 cut(s) 103, 445, 567, 1090, 1417, 1623
PflFI GACNNNGTC 1 cut(s) 1961
PflMI CCANNNNNTGG 1 cut(s) 2080
PfoI TCCNGGA 2 cut(s) 1104, 1912
PkrI GCNGC 6 cut(s) 503, 506, 1053, 1148, 1274, 1493
PleI GAGTC 5 cut(s) 577, 699, 749, 1082, 1644
PpsI GAGTC 5 cut(s) 577, 699, 749, 1082, 1644
Ppu21I YACGTR 2 cut(s) 187, 923
PscI ACATGT 1 cut(s) 1708
Psp6I CCWGG 3 cut(s) 865, 1988, 2134
PspFI CCCAGC 1 cut(s) 1902
PspGI CCWGG 3 cut(s) 865, 1988, 2134
PspN4I GGNNCC 4 cut(s) 42, 733, 1994, 2133
PspPI GGNCC 3 cut(s) 468, 1102, 1549
PstNI CAGNNNCTG 1 cut(s) 1426
PsuI RGATCY 2 cut(s) 731, 1360
PsyI GACNNNGTC 1 cut(s) 1961
PvuII CAGCTG 2 cut(s) 504, 1371
RsaI GTAC 6 cut(s) 177, 402, 1698, 1720, 2018, 2070
RsaNI GTAC 6 cut(s) 176, 401, 1697, 1719, 2017, 2069
SalI GTCGAC 1 cut(s) 752
SaqAI TTAA 8 cut(s) 147, 405, 588, 935, 1386, 1409, 1908, 2155
SatI GCNGC 6 cut(s) 502, 505, 1052, 1147, 1273, 1492
Sau3AI GATC 9 cut(s) 247, 313, 731, 928, 1304, 1360, 1374, 1683, 2085
Sau96I GGNCC 3 cut(s) 468, 1102, 1549
ScaI AGTACT 3 cut(s) 402, 1698, 2018
SchI GAGTC 5 cut(s) 577, 699, 749, 1083, 1645
ScrFI CCNGG 6 cut(s) 653, 867, 1106, 1914, 1990, 2136
SduI GDGCHC 3 cut(s) 43, 653, 980
SfaNI GCATC 7 cut(s) 55, 339, 516, 752, 1022, 1406, 1953
SfcI CTRYAG 1 cut(s) 726
SfuI TTCGAA 1 cut(s) 1765
SinI GGWCC 3 cut(s) 468, 1102, 1549
SmlI CTYRAG 6 cut(s) 665, 979, 1385, 1408, 1667, 2012
SmoI CTYRAG 6 cut(s) 665, 979, 1385, 1408, 1667, 2012
SseBI AGGCCT 2 cut(s) 960, 2004
SsiI CCGC 4 cut(s) 863, 1016, 1273, 1557
StuI AGGCCT 2 cut(s) 960, 2004
StyD4I CCNGG 6 cut(s) 651, 865, 1104, 1912, 1988, 2134
StyI CCWWGG 2 cut(s) 21, 1552
TaaI ACNGT 3 cut(s) 575, 1339, 1726
TaiI ACGT 2 cut(s) 189, 925
TaqI TCGA 5 cut(s) 162, 753, 1765, 1788, 1947
TatI WGTACW 4 cut(s) 400, 1696, 2016, 2068
TauI GCSGC 1 cut(s) 1275
TfiI GAWTC 6 cut(s) 103, 445, 567, 1090, 1417, 1623
Tru1I TTAA 8 cut(s) 147, 405, 588, 935, 1386, 1409, 1908, 2155
Tru9I TTAA 8 cut(s) 147, 405, 588, 935, 1386, 1409, 1908, 2155
TscAI CASTG 3 cut(s) 313, 773, 1342
TseI GCWGC 5 cut(s) 501, 504, 1051, 1146, 1491
TspRI CASTG 3 cut(s) 313, 773, 1342
Tth111I GACNNNGTC 1 cut(s) 1961
Van91I CCANNNNNTGG 1 cut(s) 2080
Vha464I CTTAAG 2 cut(s) 1385, 1408
VpaK11BI GGWCC 3 cut(s) 468, 1102, 1549
XapI RAATTY 2 cut(s) 390, 1294
XbaI TCTAGA 2 cut(s) 250, 1498
XceI RCATGY 1 cut(s) 1712
XcmI CCANNNNNNNNNTGG 1 cut(s) 947
XmaJI CCTAGG 1 cut(s) 1552
XmiI GTMKAC 3 cut(s) 753, 1077, 2037
XmnI GAANNNNTTC 2 cut(s) 1597, 2111
ZrmI AGTACT 3 cut(s) 402, 1698, 2018
Zsp2I ATGCAT 1 cut(s) 70
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.