Rh4CG041100

NB-ARC domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0000540
Physical Location & Seq
Forward (+)
8267361 .. 8378248
110888 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG041100.1

Sequence Viewer

Length: 2514 bp
ATGTCGTTAATAGTGGGGGCTATGGTCCAGACTCTAACGGAGCATGTGTTCACAGCTCTAATGAACCAGGCTGAATTTGCACTTGACTTCAGCGGCCAGTTTGAGCAGTTGAAGACGGGGCTTGATCTCACCAAGGCGTTACTTGCAGACACGGAGAGTCTCAACCACAAGAACAACATTGTCAAGGCAGCATTATTTGAGCTGAGAGAAGTTATCTACAAAGCCGACGATGTACTCACTGCAGATTGCTTGGTTAGAGACGAATACAGAAAGGATGGATCTTGTTCCGGCTCCTTGTTTCACGATCCATTCTTCCTGCATCGTACGGGAAAGAAATTGAAAGACATCAATTTGCGTATGAAGGAGATAGAGCAGACCTTGGGCAAGTTTTTGAGGGCTCCTGATAACATCCATGGAGAAGATGCATACCAAGTTAGGGTGATGGTTTCACAGGACTGGAATCCAACTGAGATAATTGGGTTGGATAGCGATGTGGAGAAGATTAAGGGTTGGCTTTTCGACACTAGCAAGGTACTTTCCCACGTAGGCATTGTGGGAATGGGAGGCTTGGGGAAAACCACCATTGCTCAAAAGATATTTCATGATCTAGAAGTAGTAGACCACTTTCACAAGCTGATATGGGTGTGTGTTTCACAATCATTCAGTGCTGATCGGATTATGAGGAGCATACTAGAAGGTCTAGGAGAAAATGTCTCCGGAGCTGGTGTTACTCAGATTTTGAGCAGAATTCAGCAAGTACTTGAAGATAAGAGTTGTTTGATTGTCATGGATGATGTGTGGAATCAAACAGATATTGATTGGTGGACCAACCTCTGCTCTGTTATACCCAAACAACGCAGCTGCATCATCGTTACAACTAGAAATGAAGAAGCTGTAATTGGTATGGGAGTTGAAAGTTCACGAATCCACCGTCCCAAAACTCTTAATGTTGAGGAAAGCTGGCGCTTGTTCTCCAAGTTTGCATTTCCTACAAGCAAGGGATTGTGCCAGGAAGATAGGCTTGAGAAATTAGGAAAAGAACTCTTGAAGAAGTGTGGTGGACTCCCACTCGCAATCAAGACTATAGGATCCTTGTTGGCATCAAAGGTCGACTCCCCTTCTCAGTGGAGACGAGTTCTGGAGAGCTTTCATGCTTTGACAACAGAGGGAAAAACTAGCTCAGTTATGGCCTCTTTGCGATTGAGCTATGATGAACTCCCGCCCGGTCTGAAGCAATGCCTGCTGTGTTTCTCCATCTATCCAGAAGACTATGAGATACGTGCTGATCAGTTAATCCATTGGTGGATTGGAGAAGGCCTCGTGCAAAGCAAGGGCTCAAGAACAGCAGTAGAAGTGGGATATGAGTATCTTGCGGAACTGATAAGCAGATGCTTGGTTGAAATTGTGCAGCAGAGAGGATTTGATGGGAGAGTCTACAAGTGCAAGATTCATGATATGGTCCGGGAACTGATAATAATGATTGCAGAGGAGGAGGAATTATGCGGCTTTGACGAACAAGGCAGACAGAAATTGACGGCAAATACTAGGTGGCTATGCTTTATTGATGAAATGGAGGAGAAGTCCTTGAAACGTAGTTCAAAGCTCAGGGCATTGTTGATGATGTCAAGCCGCAGATTTGACTTTGAAAGGAATTCTTTACGATCCCTCAGAATGTTGGATTTATCAAACTGCACTGTGGATGAGCATTGTGTGAAGGATCTCTTCAGCTGGATCAGCTCCCTTAAGCGCTTAGCATCTTTGAACTTAAGTGGTATTCAGGCTCTGAAAGAACTGCCATCTTCAATTCATAAACTCCTCAACCTCCAACTTTTACTTCTAACAGGTTGCAGCAATCTAGAAAAAATACACCCTTCCATCACTAATTTGAAGAAGCTGATTATTTTGGACCTAGGCGGGTGTCCAATCCAATACTTACCTCAGGGATTGGGAAGGCTTTCTTATCTTCAAGAGTTCTCCGGATTCAAGATTGTGAGTCAGCCCAGAATGCCATGTTTCCGGCTTCTTGAGATTAGGGAACTGATCAACCTGAGAGTACTGCGAATACATGTAAGCAGTACCACAGTTATTGCAGACAATGAACTTGATGTCCTCTCTCAGCTTCGAAGGCTCAATGTTCTGGCTATCGACGCAGAAGATTGTAGAAATAACAATGTTTTGGAAATGCTAGAAAGGCTGACCCCTCCTCCAAGCCTCCAAGAGCTGTATCTTAGGAATTACAAGAAGGAAACTCTGCCTAGCTGGGTTAATCCCGGACAGCTTTCAAGATTGCAGTATCTTTGCATCGAGAATGGAGACCTTGTCAAGCTAAGTAGTGGTCAAACCTCCTGGAACCTTGAAGGCCTGTGTCTCAAGTACTTGATGAGGCTAGAGGTAGACTGGAAGGACTTGGAGAAGGATACGCCTGTACTCCACTACATGGAGATCAGTCATTGTTACAACTTGAAGGATTTCCCATGTTCAGTTATGGAACCAGGGGTCTGGAGAAAGAATTAA

Protein Analysis

837

Amino Acids

95.43

Weight (kDa)

6.3

Isoelectric Point (pI)

41.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 8 - 80 2.8e-10 Rx N-terminal domain
NB-ARC PF00931 163 - 332 8.1e-40 NB-ARC domain
AAA_22 PF13401 184 - 266 4.3e-06 AAA domain
WHD_DRP PF23559 419 - 491 2.2e-20 Disease resistance protein Winged helix domain
LRR_14 PF23598 548 - 769 2e-27 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 689 - 778 1.7e-08 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000540)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G50950 AT3G50950
fragaria_vesca FvH4_1g12710 FvH4_1g12710 FvH4_3g00820 FvH4_3g00820 FvH4_3g00820 FvH4_3g00820
malus_domestica MD02G1141100.v1.1 MD10G1333100.v1.1
prunus_persica Prupe.4G009300_v2.0.a1 Prupe.4G009300_v2.0.a1 Prupe.4G009300_v2.0.a1 Prupe.6G150700_v2.0.a1 Prupe.7G160100_v2.0.a1 Prupe.7G160100_v2.0.a1
pyrus_communis pycom02g11110 pycom10g28240 pycom10g28340 pycom10g28360 pycom10g28450
rosa_chinensis RchiOBHm_Chr2g0100711 RchiOBHm_Chr3g0485861 RchiOBHm_Chr4g0391451 RchiOBHm_Chr4g0391591 RchiOBHm_Chr4g0391601 RchiOBHm_Chr4g0391681 RchiOBHm_Chr5g0001141 RchiOBHm_Chr5g0001221
rosa_laevigata RLG00000017001
rosa_multiflora Rmu_co8260007.1_g000001 Rmu_co8260103.1_g000001 Rmu_co8519155.1_g000001 Rmu_sc0000060.1_g000050 Rmu_sc0000255.1_g000036 Rmu_sc0001174.1_g000023 Rmu_sc0001174.1_g000025 Rmu_sc0001659.1_g000009 Rmu_sc0001706.1_g000009 Rmu_sc0001706.1_g000024 Rmu_sc0001706.1_g000035 Rmu_sc0004189.1_g000018 Rmu_sc0004189.1_g000030 Rmu_sc0004862.1_g000002 Rmu_sc0004876.1_g000038 Rmu_sc0023741.1_g000004
rosa_roxburghii Rroxscaffold_1G00075100 Rroxscaffold_1G00075160 Rroxscaffold_1G00075210 Rroxscaffold_5G00337160 Rroxscaffold_5G00386590 Rroxscaffold_6G00396300
rosa_rugosa Rorug03G0336200 Rorug03G0336300 Rorug04G0389400
rosa_samantha Rh2AG140400 Rh2BG144100 Rh2CG145400 Rh2DG144900 Rh3AG271200 Rh4AG038000 Rh4AG038700 Rh4BG032000 Rh4BG032800 Rh4CG041100 Rh4CG041300 Rh4DG035500 Rh4DG035600 Rh5AG008800 Rh5AG009100 Rh5BG010900 Rh5BG011500 Rh5BG011600 Rh5BG012000 Rh5CG009400 Rh5CG010000 Rh5DG009800
rosa_wichuraiana Rw2G010940 Rw4G002950 Rw4G002990 Rw4G003000 Rw5G000880 Rw5G000950 Rw5G000960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 2437
AccI GTMKAC 4 cut(s) 618, 1110, 1434, 2394
AccIII TCCGGA 2 cut(s) 716, 1976
AciI CCGC 6 cut(s) 93, 1220, 1373, 1503, 1630, 1914
AclWI GGATC 7 cut(s) 286, 299, 1083, 1096, 1656, 1725, 1739
AcoI YGGCCR 1 cut(s) 94
AcsI RAATTY 3 cut(s) 74, 747, 1651
AcuI CTGAAG 3 cut(s) 73, 1250, 1708
AfaI GTAC 8 cut(s) 234, 325, 534, 759, 2055, 2077, 2375, 2427
AfeI AGCGCT 1 cut(s) 1748
AfiI CCNNNNNNNGG 2 cut(s) 436, 2437
AflII CTTAAG 2 cut(s) 1742, 1765
AflIII ACRYGT 1 cut(s) 2065
AjnI CCWGG 4 cut(s) 66, 1008, 2345, 2491
AjuI GAANNNNNNNTTGG 4 cut(s) 882, 914, 1818, 1850
AloI GAACNNNNNNTCC 4 cut(s) 32, 64, 2091, 2123
Alw26I GTCTC 6 cut(s) 164, 252, 718, 1123, 2307, 2372
AlwI GGATC 7 cut(s) 286, 299, 1083, 1096, 1656, 1725, 1739
AlwNI CAGNNNCTG 1 cut(s) 1783
Aor13HI TCCGGA 2 cut(s) 716, 1976
Aor51HI AGCGCT 1 cut(s) 1748
AoxI GGCC 4 cut(s) 94, 1188, 1315, 2359
ApeKI GCWGC 5 cut(s) 188, 858, 861, 1408, 1848
ApoI RAATTY 3 cut(s) 74, 747, 1651
ArsI GACNNNNNNTTYG 2 cut(s) 916, 948
Asp700I GAANNNNTTC 2 cut(s) 1954, 2468
AspA2I CCTAGG 1 cut(s) 1909
AspLEI GCGC 2 cut(s) 966, 1749
AspS9I GGNCC 4 cut(s) 25, 825, 1459, 1906
AsuC2I CCSGG 3 cut(s) 1224, 1463, 2271
AsuHPI GGTGA 2 cut(s) 121, 451
AsuII TTCGAA 1 cut(s) 2122
AvaII GGWCC 4 cut(s) 25, 825, 1459, 1906
AvrII CCTAGG 1 cut(s) 1909
AxyI CCTNAGG 1 cut(s) 1938
BaeI ACNNNNGTAYC 2 cut(s) 2409, 2442
BamHI GGATCC 1 cut(s) 1088
BanII GRGCYC 2 cut(s) 400, 1337
BauI CACGAG 1 cut(s) 1319
BbsI GAAGAC 2 cut(s) 119, 1272
BbvI GCAGC 5 cut(s) 200, 848, 870, 1420, 1860
BccI CCATC 6 cut(s) 269, 436, 1262, 1418, 1804, 1883
BceAI ACGGC 1 cut(s) 1551
BciT130I CCWGG 4 cut(s) 68, 1010, 2347, 2493
BciVI GTATCC 1 cut(s) 2410
BclI TGATCA 2 cut(s) 1285, 2040
BcnI CCSGG 3 cut(s) 1224, 1463, 2271
BcoDI GTCTC 6 cut(s) 164, 252, 718, 1123, 2307, 2372
BfmI CTRYAG 2 cut(s) 240, 1083
BfoI RGCGCY 2 cut(s) 967, 1750
BfrI CTTAAG 2 cut(s) 1742, 1765
BfuI GTATCC 1 cut(s) 2410
BisI GCNGC 8 cut(s) 94, 189, 859, 862, 1409, 1504, 1630, 1849
BlnI CCTAGG 1 cut(s) 1909
BlpI GCTNAGC 1 cut(s) 1750
BlsI GCNGC 8 cut(s) 95, 190, 860, 863, 1410, 1505, 1631, 1850
BmcAI AGTACT 3 cut(s) 759, 2055, 2375
Bme1390I CCNGG 7 cut(s) 68, 1010, 1224, 1463, 2271, 2347, 2493
Bme18I GGWCC 4 cut(s) 25, 825, 1459, 1906
BmgT120I GGNCC 4 cut(s) 25, 825, 1459, 1906
BmiI GGNNCC 5 cut(s) 292, 399, 1090, 2351, 2490
BmrFI CCNGG 7 cut(s) 68, 1010, 1224, 1463, 2271, 2347, 2493
BmsI GCATC 7 cut(s) 328, 412, 873, 1109, 1379, 1763, 2310
BpiI GAAGAC 2 cut(s) 119, 1272
BplI GAGNNNNNCTC 2 cut(s) 1302, 1334
BpmI CTGGAG 1 cut(s) 1160
Bpu10I CCTNAGC 1 cut(s) 1604
Bpu1102I GCTNAGC 1 cut(s) 1750
Bpu14I TTCGAA 1 cut(s) 2122
BpuEI CTTGAG 4 cut(s) 1043, 1321, 2045, 2354
BpuMI CCSGG 3 cut(s) 1224, 1463, 2271
BsaAI YACGTR 2 cut(s) 544, 1280
BsaI GGTCTC 1 cut(s) 2307
BsaJI CCNNGG 5 cut(s) 132, 378, 412, 1909, 2492
BsaWI WCCGGW 2 cut(s) 716, 1976
Bsc4I CCNNNNNNNGG 2 cut(s) 436, 2437
Bse1I ACTGG 3 cut(s) 97, 461, 2402
Bse21I CCTNAGG 1 cut(s) 1938
Bse3DI GCAATG 2 cut(s) 582, 1241
BseAI TCCGGA 2 cut(s) 716, 1976
BseBI CCWGG 4 cut(s) 68, 1010, 2347, 2493
BseDI CCNNGG 5 cut(s) 132, 378, 412, 1909, 2492
BseGI GGATG 4 cut(s) 280, 408, 796, 1705
BseLI CCNNNNNNNGG 2 cut(s) 436, 2437
BseMI GCAATG 2 cut(s) 582, 1241
BseNI ACTGG 3 cut(s) 97, 461, 2402
BseRI GAGGAG 6 cut(s) 697, 1502, 1505, 1589, 1805, 2193
BseXI GCAGC 5 cut(s) 200, 848, 870, 1420, 1860
BseYI CCCAGC 1 cut(s) 2259
BsgI GTGCAG 2 cut(s) 1427, 1675
BshFI GGCC 4 cut(s) 96, 1190, 1317, 2361
BsiSI CCGG 7 cut(s) 288, 717, 1224, 1462, 1977, 2017, 2271
BsiWI CGTACG 1 cut(s) 323
BslFI GGGAC 1 cut(s) 918
BslI CCNNNNNNNGG 2 cut(s) 436, 2437
BsmAI GTCTC 6 cut(s) 164, 252, 718, 1123, 2307, 2372
BsmBI CGTCTC 2 cut(s) 252, 1123
BsmFI GGGAC 1 cut(s) 918
BsmI GAATGC 1 cut(s) 2010
BsnI GGCC 4 cut(s) 96, 1190, 1317, 2361
Bso31I GGTCTC 1 cut(s) 2307
Bsp119I TTCGAA 1 cut(s) 2122
Bsp1286I GDGCHC 2 cut(s) 400, 1337
Bsp13I TCCGGA 2 cut(s) 716, 1976
Bsp1720I GCTNAGC 1 cut(s) 1750
Bsp19I CCATGG 1 cut(s) 412
BspACI CCGC 6 cut(s) 93, 1220, 1373, 1503, 1630, 1914
BspANI GGCC 4 cut(s) 96, 1190, 1317, 2361
BspEI TCCGGA 2 cut(s) 716, 1976
BspHI TCATGA 2 cut(s) 601, 1450
BspLI GGNNCC 5 cut(s) 292, 399, 1090, 2351, 2490
BspMAI CTGCAG 1 cut(s) 244
BspPI GGATC 7 cut(s) 286, 299, 1083, 1096, 1656, 1725, 1739
BspT104I TTCGAA 1 cut(s) 2122
BspTI CTTAAG 2 cut(s) 1742, 1765
BspTNI GGTCTC 1 cut(s) 2307
BsrDI GCAATG 2 cut(s) 582, 1241
BsrI ACTGG 3 cut(s) 97, 461, 2402
BssECI CCNNGG 5 cut(s) 132, 378, 412, 1909, 2492
BssSI CACGAG 1 cut(s) 1319
BssT1I CCWWGG 4 cut(s) 132, 378, 412, 1909
Bst2BI CACGAG 1 cut(s) 1319
Bst2UI CCWGG 4 cut(s) 68, 1010, 2347, 2493
Bst4CI ACNGT 3 cut(s) 932, 1696, 2083
Bst6I CTCTTC 1 cut(s) 1727
BstAFI CTTAAG 2 cut(s) 1742, 1765
BstAPI GCANNNNNTGC 1 cut(s) 1240
BstBAI YACGTR 2 cut(s) 544, 1280
BstBI TTCGAA 1 cut(s) 2122
BstC8I GCNNGC 2 cut(s) 962, 1241
BstDSI CCRYGG 1 cut(s) 412
BstF5I GGATG 4 cut(s) 280, 408, 796, 1705
BstH2I RGCGCY 2 cut(s) 967, 1750
BstHHI GCGC 2 cut(s) 966, 1749
BstMAI GTCTC 6 cut(s) 164, 252, 718, 1123, 2307, 2372
BstMWI GCNNNNNNNGC 8 cut(s) 77, 143, 1240, 1734, 2005, 2125, 2147, 2191
BstNI CCWGG 4 cut(s) 68, 1010, 2347, 2493
BstNSI RCATGY 2 cut(s) 47, 2069
BstSCI CCNGG 7 cut(s) 66, 1008, 1222, 1461, 2269, 2345, 2491
BstSFI CTRYAG 2 cut(s) 240, 1083
BstV1I GCAGC 5 cut(s) 200, 848, 870, 1420, 1860
BstV2I GAAGAC 2 cut(s) 119, 1272
BstX2I RGATCY 3 cut(s) 278, 1088, 1717
BstXI CCANNNNNNTGG 1 cut(s) 2499
BstYI RGATCY 3 cut(s) 278, 1088, 1717
Bsu36I CCTNAGG 1 cut(s) 1938
BsuI GTATCC 1 cut(s) 2410
BsuRI GGCC 4 cut(s) 96, 1190, 1317, 2361
BtgI CCRYGG 1 cut(s) 412
BtgZI GCGATG 1 cut(s) 504
BtsCI GGATG 4 cut(s) 280, 408, 796, 1705
BtsI GCAGTG 1 cut(s) 237
BtsIMutI CAGTG 4 cut(s) 237, 670, 1130, 1692
Cac8I GCNNGC 2 cut(s) 962, 1241
CaiI CAGNNNCTG 1 cut(s) 1783
CciI TCATGA 2 cut(s) 601, 1450
CfoI GCGC 2 cut(s) 966, 1749
Cfr13I GGNCC 4 cut(s) 25, 825, 1459, 1906
CseI GACGC 1 cut(s) 2156
Csp6I GTAC 8 cut(s) 233, 324, 533, 758, 2054, 2076, 2374, 2426
CspCI CAANNNNNGTGG 2 cut(s) 2068, 2103
CviQI GTAC 8 cut(s) 233, 324, 533, 758, 2054, 2076, 2374, 2426
EaeI YGGCCR 1 cut(s) 94
Eam1104I CTCTTC 1 cut(s) 1727
EarI CTCTTC 1 cut(s) 1727
Eco130I CCWWGG 4 cut(s) 132, 378, 412, 1909
Eco147I AGGCCT 2 cut(s) 1317, 2361
Eco24I GRGCYC 2 cut(s) 400, 1337
Eco31I GGTCTC 1 cut(s) 2307
Eco47I GGWCC 4 cut(s) 25, 825, 1459, 1906
Eco47III AGCGCT 1 cut(s) 1748
Eco57I CTGAAG 3 cut(s) 73, 1250, 1708
Eco81I CCTNAGG 1 cut(s) 1938
EcoRI GAATTC 2 cut(s) 747, 1651
EcoRII CCWGG 4 cut(s) 66, 1008, 2345, 2491
EcoT14I CCWWGG 4 cut(s) 132, 378, 412, 1909
EcoT22I ATGCAT 1 cut(s) 427
EcoT38I GRGCYC 2 cut(s) 400, 1337
ErhI CCWWGG 4 cut(s) 132, 378, 412, 1909
Esp3I CGTCTC 2 cut(s) 252, 1123
FalI AAGNNNNNCTT 8 cut(s) 1005, 1037, 1639, 1671, 1706, 1738, 1942, 1974
FaqI GGGAC 1 cut(s) 918
FauI CCCGC 2 cut(s) 1227, 1907
FbaI TGATCA 2 cut(s) 1285, 2040
FblI GTMKAC 4 cut(s) 618, 1110, 1434, 2394
Fnu4HI GCNGC 8 cut(s) 94, 189, 859, 862, 1409, 1504, 1630, 1849
FokI GGATG 4 cut(s) 287, 395, 803, 1712
FriOI GRGCYC 2 cut(s) 400, 1337
Fsp4HI GCNGC 8 cut(s) 94, 189, 859, 862, 1409, 1504, 1630, 1849
GlaI GCGC 2 cut(s) 965, 1748
GluI GCNGC 8 cut(s) 94, 189, 859, 862, 1409, 1504, 1630, 1849
GsaI CCCAGC 1 cut(s) 2263
GsuI CTGGAG 1 cut(s) 1160
HaeII RGCGCY 2 cut(s) 967, 1750
HaeIII GGCC 4 cut(s) 96, 1190, 1317, 2361
HapII CCGG 7 cut(s) 288, 717, 1224, 1462, 1977, 2017, 2271
HgaI GACGC 1 cut(s) 2156
HhaI GCGC 2 cut(s) 966, 1749
Hin6I GCGC 2 cut(s) 964, 1747
HinP1I GCGC 2 cut(s) 964, 1747
HincII GTYRAC 1 cut(s) 1111
HindII GTYRAC 1 cut(s) 1111
HpaII CCGG 7 cut(s) 288, 717, 1224, 1462, 1977, 2017, 2271
HphI GGTGA 2 cut(s) 121, 451
Hpy166II GTNNAC 8 cut(s) 51, 619, 825, 920, 1061, 1111, 1435, 2395
Hpy188I TCNGA 5 cut(s) 675, 735, 1230, 1670, 1785
Hpy8I GTNNAC 8 cut(s) 51, 619, 825, 920, 1061, 1111, 1435, 2395
Hpy99I CGWCG 2 cut(s) 230, 2150
HpyCH4III ACNGT 3 cut(s) 932, 1696, 2083
HpyCH4IV ACGT 3 cut(s) 543, 1279, 1591
HpyF10VI GCNNNNNNNGC 8 cut(s) 77, 143, 1240, 1734, 2005, 2125, 2147, 2191
HpySE526I ACGT 3 cut(s) 543, 1279, 1591
HspAI GCGC 2 cut(s) 964, 1747
Kpn2I TCCGGA 2 cut(s) 716, 1976
Ksp22I TGATCA 2 cut(s) 1285, 2040
LmnI GCTCC 6 cut(s) 40, 296, 403, 684, 719, 1742
Lsp1109I GCAGC 5 cut(s) 200, 848, 870, 1420, 1860
LweI GCATC 7 cut(s) 328, 412, 873, 1109, 1379, 1763, 2310
MaeII ACGT 3 cut(s) 543, 1279, 1591
MaeIII GTNAC 4 cut(s) 138, 727, 871, 2453
MflI RGATCY 3 cut(s) 278, 1088, 1717
MhlI GDGCHC 2 cut(s) 400, 1337
MlyI GAGTC 6 cut(s) 25, 166, 1056, 1106, 1440, 2002
MmeI TCCRAC 4 cut(s) 462, 488, 1656, 1849
Mph1103I ATGCAT 1 cut(s) 427
MroI TCCGGA 2 cut(s) 716, 1976
MroXI GAANNNNTTC 2 cut(s) 1954, 2468
MseI TTAA 8 cut(s) 8, 504, 945, 1292, 1743, 1766, 2265, 2512
MspA1I CMGCKG 3 cut(s) 93, 861, 1728
MspCI CTTAAG 2 cut(s) 1742, 1765
MspI CCGG 7 cut(s) 288, 717, 1224, 1462, 1977, 2017, 2271
MspR9I CCNGG 7 cut(s) 68, 1010, 1224, 1463, 2271, 2347, 2493
Mva1269I GAATGC 1 cut(s) 2010
MvaI CCWGG 4 cut(s) 68, 1010, 2347, 2493
MwoI GCNNNNNNNGC 8 cut(s) 77, 143, 1240, 1734, 2005, 2125, 2147, 2191
NciI CCSGG 3 cut(s) 1224, 1463, 2271
NcoI CCATGG 1 cut(s) 412
NlaIV GGNNCC 5 cut(s) 292, 399, 1090, 2351, 2490
NsiI ATGCAT 1 cut(s) 427
NspI RCATGY 2 cut(s) 47, 2069
NspV TTCGAA 1 cut(s) 2122
PagI TCATGA 2 cut(s) 601, 1450
PceI AGGCCT 2 cut(s) 1317, 2361
PciI ACATGT 1 cut(s) 2065
PcsI WCGNNNNNNNCGW 1 cut(s) 928
PctI GAATGC 1 cut(s) 2010
PdmI GAANNNNTTC 2 cut(s) 1954, 2468
PfeI GAWTC 5 cut(s) 460, 802, 924, 1447, 1980
Pfl23II CGTACG 1 cut(s) 323
PflFI GACNNNGTC 1 cut(s) 2318
PflMI CCANNNNNTGG 1 cut(s) 2437
PfoI TCCNGGA 3 cut(s) 1461, 2269, 2345
PkrI GCNGC 8 cut(s) 95, 190, 860, 863, 1410, 1505, 1631, 1850
PleI GAGTC 6 cut(s) 25, 165, 1056, 1106, 1439, 2001
PpsI GAGTC 6 cut(s) 25, 165, 1056, 1106, 1439, 2001
Ppu21I YACGTR 2 cut(s) 544, 1280
PscI ACATGT 1 cut(s) 2065
Psp6I CCWGG 4 cut(s) 66, 1008, 2345, 2491
PspFI CCCAGC 1 cut(s) 2259
PspGI CCWGG 4 cut(s) 66, 1008, 2345, 2491
PspLI CGTACG 1 cut(s) 323
PspN4I GGNNCC 5 cut(s) 292, 399, 1090, 2351, 2490
PspPI GGNCC 4 cut(s) 25, 825, 1459, 1906
PstI CTGCAG 1 cut(s) 244
PstNI CAGNNNCTG 1 cut(s) 1783
PsuI RGATCY 3 cut(s) 278, 1088, 1717
PsyI GACNNNGTC 1 cut(s) 2318
PvuII CAGCTG 2 cut(s) 861, 1728
RsaI GTAC 8 cut(s) 234, 325, 534, 759, 2055, 2077, 2375, 2427
RsaNI GTAC 8 cut(s) 233, 324, 533, 758, 2054, 2076, 2374, 2426
SalI GTCGAC 1 cut(s) 1109
SaqAI TTAA 8 cut(s) 8, 504, 945, 1292, 1743, 1766, 2265, 2512
SatI GCNGC 8 cut(s) 94, 189, 859, 862, 1409, 1504, 1630, 1849
Sau96I GGNCC 4 cut(s) 25, 825, 1459, 1906
ScaI AGTACT 3 cut(s) 759, 2055, 2375
SchI GAGTC 6 cut(s) 25, 166, 1056, 1106, 1440, 2002
ScrFI CCNGG 7 cut(s) 68, 1010, 1224, 1463, 2271, 2347, 2493
SduI GDGCHC 2 cut(s) 400, 1337
SfaNI GCATC 7 cut(s) 328, 412, 873, 1109, 1379, 1763, 2310
SfcI CTRYAG 2 cut(s) 240, 1083
SfuI TTCGAA 1 cut(s) 2122
SinI GGWCC 4 cut(s) 25, 825, 1459, 1906
SmlI CTYRAG 6 cut(s) 1022, 1336, 1742, 1765, 2024, 2369
SmoI CTYRAG 6 cut(s) 1022, 1336, 1742, 1765, 2024, 2369
SseBI AGGCCT 2 cut(s) 1317, 2361
SsiI CCGC 6 cut(s) 93, 1220, 1373, 1503, 1630, 1914
StuI AGGCCT 2 cut(s) 1317, 2361
StyD4I CCNGG 7 cut(s) 66, 1008, 1222, 1461, 2269, 2345, 2491
StyI CCWWGG 4 cut(s) 132, 378, 412, 1909
TaaI ACNGT 3 cut(s) 932, 1696, 2083
TaiI ACGT 3 cut(s) 546, 1282, 1594
TaqI TCGA 5 cut(s) 519, 1110, 2122, 2145, 2304
TatI WGTACW 5 cut(s) 232, 757, 2053, 2373, 2425
TauI GCSGC 3 cut(s) 96, 1506, 1632
TfiI GAWTC 5 cut(s) 460, 802, 924, 1447, 1980
Tru1I TTAA 8 cut(s) 8, 504, 945, 1292, 1743, 1766, 2265, 2512
Tru9I TTAA 8 cut(s) 8, 504, 945, 1292, 1743, 1766, 2265, 2512
TscAI CASTG 4 cut(s) 244, 670, 1130, 1699
TseI GCWGC 5 cut(s) 188, 858, 861, 1408, 1848
TspGWI ACGGA 2 cut(s) 53, 167
TspRI CASTG 4 cut(s) 244, 670, 1130, 1699
Tth111I GACNNNGTC 1 cut(s) 2318
Van91I CCANNNNNTGG 1 cut(s) 2437
Vha464I CTTAAG 2 cut(s) 1742, 1765
VpaK11BI GGWCC 4 cut(s) 25, 825, 1459, 1906
XapI RAATTY 3 cut(s) 74, 747, 1651
XbaI TCTAGA 2 cut(s) 607, 1855
XceI RCATGY 2 cut(s) 47, 2069
XcmI CCANNNNNNNNNTGG 1 cut(s) 1304
XmaJI CCTAGG 1 cut(s) 1909
XmiI GTMKAC 4 cut(s) 618, 1110, 1434, 2394
XmnI GAANNNNTTC 2 cut(s) 1954, 2468
ZrmI AGTACT 3 cut(s) 759, 2055, 2375
Zsp2I ATGCAT 1 cut(s) 427
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.