Rh5BG011600

NB-ARC domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0000540
Physical Location & Seq
Forward (+)
829688 .. 830587
900 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG011600.1

Sequence Viewer

Length: 900 bp
ATGTCAAGCCGCAGATTTGACTTTGAAAGGAATTCGGGGTTTCTGCGATCCCTCAGAATGTTGGACCTATCAAACTGCACTGTGGATGAGCATTGTGTGGAGGATCTCTTCAGCTGGATCAGCTCCCTTAAGCGCTTGGCATCTCTGAACTTAAGTGGTATTCAAGCCCTGAAAGAAGTGCCTTCTTCAATTCATAAACTCCTCAACCTCCAACTTTTACTTTTAACAGGTTGCAGCAATCTAGTAAAAATACACCATTCCATCACTAATTTGAAGAAGCTGATTATTTTGGACCTAGGCGGGTGTCCAATCCAATACTTGCCCCAGGGATTGGGAAGGCTTTCTTATCTTCAAGAGCTCTCCGGATTCAAGGTTGTGAGTCAGCCTAGAAAGCCAAGTTTCCGGCTTCTTGAGATTAGGGAATTGATCAACCTGAGAGTACTGCGAATACATGTAAGCAGTACCACAGTTATCGCAGACAATGAACTTGATGTCCTCTCTCAGCTTAGAAGGCTCAATGTTCTAGCTATTGATGCAGAAGATTGTAGAAATAACAATGTTTTGGAAATGCTAGAAGAGCTGACCCCTCCTCCAAGCCTCCAAGAGCTATATCTTAGGAATTACAAGAAGGAAACTCTGCCCAACTGGGTTAATCCTGGACAACTTTCACGATTGCAGTATCTTTGCATCGAGAATGGAGACCTTGTCAAGCTAAGCAGTGGTCAAACCACCTGGAATCTTGAAGGCCTGTGTCTCAAGTACTTGATGAGGCTAGAGGTAGACTGGAAGGACTTGGAGAAGGATATGCCTGTACTTCACTACATGGAGATCAGTCATTGTTACAACTTGACGGATTTCCCATGTTCGGTTATGGAACCTGGGGTCTGGAGAAAGCATTAA

Protein Analysis

299

Amino Acids

34.43

Weight (kDa)

7.07

Isoelectric Point (pI)

48.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 16 - 231 5.8e-28 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 151 - 240 3.2e-09 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000540)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G50950 AT3G50950
fragaria_vesca FvH4_1g12710 FvH4_1g12710 FvH4_3g00820 FvH4_3g00820 FvH4_3g00820 FvH4_3g00820
malus_domestica MD02G1141100.v1.1 MD10G1333100.v1.1
prunus_persica Prupe.4G009300_v2.0.a1 Prupe.4G009300_v2.0.a1 Prupe.4G009300_v2.0.a1 Prupe.6G150700_v2.0.a1 Prupe.7G160100_v2.0.a1 Prupe.7G160100_v2.0.a1
pyrus_communis pycom02g11110 pycom10g28240 pycom10g28340 pycom10g28360 pycom10g28450
rosa_chinensis RchiOBHm_Chr2g0100711 RchiOBHm_Chr3g0485861 RchiOBHm_Chr4g0391451 RchiOBHm_Chr4g0391591 RchiOBHm_Chr4g0391601 RchiOBHm_Chr4g0391681 RchiOBHm_Chr5g0001141 RchiOBHm_Chr5g0001221
rosa_laevigata RLG00000017001
rosa_multiflora Rmu_co8260007.1_g000001 Rmu_co8260103.1_g000001 Rmu_co8519155.1_g000001 Rmu_sc0000060.1_g000050 Rmu_sc0000255.1_g000036 Rmu_sc0001174.1_g000023 Rmu_sc0001174.1_g000025 Rmu_sc0001659.1_g000009 Rmu_sc0001706.1_g000009 Rmu_sc0001706.1_g000024 Rmu_sc0001706.1_g000035 Rmu_sc0004189.1_g000018 Rmu_sc0004189.1_g000030 Rmu_sc0004862.1_g000002 Rmu_sc0004876.1_g000038 Rmu_sc0023741.1_g000004
rosa_roxburghii Rroxscaffold_1G00075100 Rroxscaffold_1G00075160 Rroxscaffold_1G00075210 Rroxscaffold_5G00337160 Rroxscaffold_5G00386590 Rroxscaffold_6G00396300
rosa_rugosa Rorug03G0336200 Rorug03G0336300 Rorug04G0389400
rosa_samantha Rh2AG140400 Rh2BG144100 Rh2CG145400 Rh2DG144900 Rh3AG271200 Rh4AG038000 Rh4AG038700 Rh4BG032000 Rh4BG032800 Rh4CG041100 Rh4CG041300 Rh4DG035500 Rh4DG035600 Rh5AG008800 Rh5AG009100 Rh5BG010900 Rh5BG011500 Rh5BG011600 Rh5BG012000 Rh5CG009400 Rh5CG010000 Rh5DG009800
rosa_wichuraiana Rw2G010940 Rw4G002950 Rw4G002990 Rw4G003000 Rw5G000880 Rw5G000950 Rw5G000960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 331
AccI GTMKAC 1 cut(s) 780
AccIII TCCGGA 1 cut(s) 362
AciI CCGC 2 cut(s) 10, 300
AclWI GGATC 3 cut(s) 42, 111, 125
AcsI RAATTY 1 cut(s) 31
AcuI CTGAAG 1 cut(s) 94
AfaI GTAC 4 cut(s) 441, 463, 761, 813
AfeI AGCGCT 1 cut(s) 134
AfiI CCNNNNNNNGG 2 cut(s) 331, 865
AflII CTTAAG 2 cut(s) 128, 151
AflIII ACRYGT 1 cut(s) 451
AgsI TTSAA 7 cut(s) 26, 164, 189, 274, 353, 370, 743
AjnI CCWGG 4 cut(s) 324, 655, 731, 877
AloI GAACNNNNNNTCC 2 cut(s) 477, 509
AluBI AGCT 9 cut(s) 114, 123, 280, 358, 505, 527, 580, 607, 712
AluI AGCT 9 cut(s) 114, 123, 280, 358, 505, 527, 580, 607, 712
Alw21I GWGCWC 1 cut(s) 360
Alw26I GTCTC 2 cut(s) 693, 758
AlwI GGATC 3 cut(s) 42, 111, 125
Aor13HI TCCGGA 1 cut(s) 362
Aor51HI AGCGCT 1 cut(s) 134
AoxI GGCC 1 cut(s) 745
ApeKI GCWGC 1 cut(s) 234
ApoI RAATTY 1 cut(s) 31
Asp700I GAANNNNTTC 1 cut(s) 340
AspA2I CCTAGG 1 cut(s) 295
AspLEI GCGC 1 cut(s) 135
AspS9I GGNCC 2 cut(s) 64, 292
AvaII GGWCC 2 cut(s) 64, 292
AvrII CCTAGG 1 cut(s) 295
BanII GRGCYC 1 cut(s) 360
Bbv12I GWGCWC 1 cut(s) 360
BbvI GCAGC 1 cut(s) 246
BccI CCATC 1 cut(s) 269
BciT130I CCWGG 4 cut(s) 326, 657, 733, 879
BclI TGATCA 1 cut(s) 426
BcoDI GTCTC 2 cut(s) 693, 758
BfaI CTAG 6 cut(s) 242, 296, 387, 524, 572, 773
BfoI RGCGCY 1 cut(s) 136
BfrI CTTAAG 2 cut(s) 128, 151
BisI GCNGC 2 cut(s) 10, 235
BlnI CCTAGG 1 cut(s) 295
BlpI GCTNAGC 1 cut(s) 713
BlsI GCNGC 2 cut(s) 11, 236
BmcAI AGTACT 2 cut(s) 441, 761
Bme1390I CCNGG 4 cut(s) 326, 657, 733, 879
Bme18I GGWCC 2 cut(s) 64, 292
BmgT120I GGNCC 2 cut(s) 64, 292
BmiI GGNNCC 1 cut(s) 876
BmrFI CCNGG 4 cut(s) 326, 657, 733, 879
BmrI ACTGGG 1 cut(s) 655
BmsI GCATC 3 cut(s) 149, 523, 696
BmuI ACTGGG 1 cut(s) 655
Bpu1102I GCTNAGC 1 cut(s) 713
BpuEI CTTGAG 2 cut(s) 431, 740
BsaI GGTCTC 1 cut(s) 693
BsaJI CCNNGG 4 cut(s) 295, 324, 325, 878
BsaWI WCCGGW 1 cut(s) 362
BsaXI ACNNNNNCTCC 4 cut(s) 574, 604, 690, 720
Bsc4I CCNNNNNNNGG 2 cut(s) 331, 865
Bse1I ACTGG 2 cut(s) 650, 788
BseAI TCCGGA 1 cut(s) 362
BseBI CCWGG 4 cut(s) 326, 657, 733, 879
BseDI CCNNGG 4 cut(s) 295, 324, 325, 878
BseGI GGATG 1 cut(s) 91
BseLI CCNNNNNNNGG 2 cut(s) 331, 865
BseMII CTCAG 3 cut(s) 67, 425, 515
BseNI ACTGG 2 cut(s) 650, 788
BseRI GAGGAG 2 cut(s) 191, 579
BseXI GCAGC 1 cut(s) 246
BsgI GTGCAG 1 cut(s) 61
BshFI GGCC 1 cut(s) 747
BsiHKAI GWGCWC 1 cut(s) 360
BsiSI CCGG 2 cut(s) 363, 403
BslI CCNNNNNNNGG 2 cut(s) 331, 865
BsmAI GTCTC 2 cut(s) 693, 758
BsnI GGCC 1 cut(s) 747
Bso31I GGTCTC 1 cut(s) 693
Bsp1286I GDGCHC 1 cut(s) 360
Bsp13I TCCGGA 1 cut(s) 362
Bsp143I GATC 5 cut(s) 47, 103, 117, 426, 828
Bsp1720I GCTNAGC 1 cut(s) 713
BspACI CCGC 2 cut(s) 10, 300
BspANI GGCC 1 cut(s) 747
BspCNI CTCAG 3 cut(s) 66, 426, 514
BspEI TCCGGA 1 cut(s) 362
BspLI GGNNCC 1 cut(s) 876
BspPI GGATC 3 cut(s) 42, 111, 125
BspQI GCTCTTC 1 cut(s) 570
BspTI CTTAAG 2 cut(s) 128, 151
BspTNI GGTCTC 1 cut(s) 693
BsrI ACTGG 2 cut(s) 650, 788
BssECI CCNNGG 4 cut(s) 295, 324, 325, 878
BssMI GATC 5 cut(s) 47, 103, 117, 426, 828
BssT1I CCWWGG 1 cut(s) 295
Bst2UI CCWGG 4 cut(s) 326, 657, 733, 879
Bst4CI ACNGT 2 cut(s) 82, 469
Bst6I CTCTTC 2 cut(s) 113, 570
BstAFI CTTAAG 2 cut(s) 128, 151
BstDEI CTNAG 6 cut(s) 53, 434, 501, 506, 614, 713
BstF5I GGATG 1 cut(s) 91
BstH2I RGCGCY 1 cut(s) 136
BstHHI GCGC 1 cut(s) 135
BstKTI GATC 5 cut(s) 50, 106, 120, 429, 831
BstMAI GTCTC 2 cut(s) 693, 758
BstMBI GATC 5 cut(s) 47, 103, 117, 426, 828
BstMWI GCNNNNNNNGC 5 cut(s) 120, 391, 511, 533, 577
BstNI CCWGG 4 cut(s) 326, 657, 733, 879
BstNSI RCATGY 1 cut(s) 455
BstSCI CCNGG 4 cut(s) 324, 655, 731, 877
BstV1I GCAGC 1 cut(s) 246
BstX2I RGATCY 1 cut(s) 103
BstYI RGATCY 1 cut(s) 103
BsuRI GGCC 1 cut(s) 747
BtsCI GGATG 1 cut(s) 91
BtsI GCAGTG 1 cut(s) 724
BtsIMutI CAGTG 2 cut(s) 78, 724
CfoI GCGC 1 cut(s) 135
Cfr13I GGNCC 2 cut(s) 64, 292
Csp6I GTAC 4 cut(s) 440, 462, 760, 812
CviAII CATG 3 cut(s) 452, 823, 861
CviQI GTAC 4 cut(s) 440, 462, 760, 812
DdeI CTNAG 6 cut(s) 53, 434, 501, 506, 614, 713
DpnI GATC 5 cut(s) 49, 105, 119, 428, 830
DpnII GATC 5 cut(s) 47, 103, 117, 426, 828
Eam1104I CTCTTC 2 cut(s) 113, 570
EarI CTCTTC 2 cut(s) 113, 570
Ecl136II GAGCTC 1 cut(s) 358
Eco130I CCWWGG 1 cut(s) 295
Eco147I AGGCCT 1 cut(s) 747
Eco24I GRGCYC 1 cut(s) 360
Eco31I GGTCTC 1 cut(s) 693
Eco47I GGWCC 2 cut(s) 64, 292
Eco47III AGCGCT 1 cut(s) 134
Eco53kI GAGCTC 1 cut(s) 358
Eco57I CTGAAG 1 cut(s) 94
EcoICRI GAGCTC 1 cut(s) 358
EcoRI GAATTC 1 cut(s) 31
EcoRII CCWGG 4 cut(s) 324, 655, 731, 877
EcoT14I CCWWGG 1 cut(s) 295
EcoT38I GRGCYC 1 cut(s) 360
ErhI CCWWGG 1 cut(s) 295
FaeI CATG 3 cut(s) 455, 826, 864
FaiI YATR 7 cut(s) 195, 453, 610, 806, 824, 862, 872
FalI AAGNNNNNCTT 2 cut(s) 328, 360
FatI CATG 3 cut(s) 451, 822, 860
FauI CCCGC 1 cut(s) 293
FbaI TGATCA 1 cut(s) 426
FblI GTMKAC 1 cut(s) 780
Fnu4HI GCNGC 2 cut(s) 10, 235
FokI GGATG 1 cut(s) 98
FriOI GRGCYC 1 cut(s) 360
Fsp4HI GCNGC 2 cut(s) 10, 235
FspBI CTAG 6 cut(s) 242, 296, 387, 524, 572, 773
GlaI GCGC 1 cut(s) 134
GluI GCNGC 2 cut(s) 10, 235
HaeII RGCGCY 1 cut(s) 136
HaeIII GGCC 1 cut(s) 747
HapII CCGG 2 cut(s) 363, 403
HhaI GCGC 1 cut(s) 135
Hin1II CATG 3 cut(s) 455, 826, 864
Hin6I GCGC 1 cut(s) 133
HinP1I GCGC 1 cut(s) 133
HinfI GANTC 3 cut(s) 366, 379, 736
HpaII CCGG 2 cut(s) 363, 403
Hpy166II GTNNAC 1 cut(s) 781
Hpy188I TCNGA 2 cut(s) 56, 147
Hpy188III TCNNGA 7 cut(s) 353, 363, 410, 669, 691, 740, 886
Hpy8I GTNNAC 1 cut(s) 781
HpyAV CCTTC 7 cut(s) 192, 330, 504, 622, 737, 781, 793
HpyCH4III ACNGT 2 cut(s) 82, 469
HpyCH4V TGCA 5 cut(s) 78, 234, 536, 676, 687
HpyF10VI GCNNNNNNNGC 5 cut(s) 120, 391, 511, 533, 577
HpyF3I CTNAG 6 cut(s) 53, 434, 501, 506, 614, 713
Hsp92II CATG 3 cut(s) 455, 826, 864
HspAI GCGC 1 cut(s) 133
Kpn2I TCCGGA 1 cut(s) 362
Ksp22I TGATCA 1 cut(s) 426
Kzo9I GATC 5 cut(s) 47, 103, 117, 426, 828
LguI GCTCTTC 1 cut(s) 570
LmnI GCTCC 1 cut(s) 128
Lsp1109I GCAGC 1 cut(s) 246
LweI GCATC 3 cut(s) 149, 523, 696
MaeI CTAG 6 cut(s) 242, 296, 387, 524, 572, 773
MaeIII GTNAC 1 cut(s) 839
MalI GATC 5 cut(s) 49, 105, 119, 428, 830
MboI GATC 5 cut(s) 47, 103, 117, 426, 828
MboII GAAGA 6 cut(s) 100, 177, 286, 341, 551, 587
MflI RGATCY 1 cut(s) 103
MhlI GDGCHC 1 cut(s) 360
MluCI AATT 5 cut(s) 31, 189, 268, 422, 619
MlyI GAGTC 1 cut(s) 388
MmeI TCCRAC 2 cut(s) 42, 235
MroI TCCGGA 1 cut(s) 362
MroXI GAANNNNTTC 1 cut(s) 340
MseI TTAA 5 cut(s) 129, 152, 224, 651, 898
MspA1I CMGCKG 1 cut(s) 114
MspCI CTTAAG 2 cut(s) 128, 151
MspI CCGG 2 cut(s) 363, 403
MspR9I CCNGG 4 cut(s) 326, 657, 733, 879
MvaI CCWGG 4 cut(s) 326, 657, 733, 879
MwoI GCNNNNNNNGC 5 cut(s) 120, 391, 511, 533, 577
NdeII GATC 5 cut(s) 47, 103, 117, 426, 828
NlaIII CATG 3 cut(s) 455, 826, 864
NlaIV GGNNCC 1 cut(s) 876
NspI RCATGY 1 cut(s) 455
PasI CCCWGGG 1 cut(s) 325
PceI AGGCCT 1 cut(s) 747
PciI ACATGT 1 cut(s) 451
PciSI GCTCTTC 1 cut(s) 570
PdmI GAANNNNTTC 1 cut(s) 340
PfeI GAWTC 2 cut(s) 366, 736
PflFI GACNNNGTC 1 cut(s) 704
PflMI CCANNNNNTGG 1 cut(s) 331
PfoI TCCNGGA 1 cut(s) 655
PkrI GCNGC 2 cut(s) 11, 236
PleI GAGTC 1 cut(s) 387
PpsI GAGTC 1 cut(s) 387
PscI ACATGT 1 cut(s) 451
Psp124BI GAGCTC 1 cut(s) 360
Psp6I CCWGG 4 cut(s) 324, 655, 731, 877
PspGI CCWGG 4 cut(s) 324, 655, 731, 877
PspN4I GGNNCC 1 cut(s) 876
PspPI GGNCC 2 cut(s) 64, 292
PsuI RGATCY 1 cut(s) 103
PsyI GACNNNGTC 1 cut(s) 704
PvuII CAGCTG 1 cut(s) 114
RsaI GTAC 4 cut(s) 441, 463, 761, 813
RsaNI GTAC 4 cut(s) 440, 462, 760, 812
SacI GAGCTC 1 cut(s) 360
SapI GCTCTTC 1 cut(s) 570
SaqAI TTAA 5 cut(s) 129, 152, 224, 651, 898
SatI GCNGC 2 cut(s) 10, 235
Sau3AI GATC 5 cut(s) 47, 103, 117, 426, 828
Sau96I GGNCC 2 cut(s) 64, 292
ScaI AGTACT 2 cut(s) 441, 761
SchI GAGTC 1 cut(s) 388
ScrFI CCNGG 4 cut(s) 326, 657, 733, 879
SduI GDGCHC 1 cut(s) 360
SfaNI GCATC 3 cut(s) 149, 523, 696
SinI GGWCC 2 cut(s) 64, 292
SmlI CTYRAG 4 cut(s) 128, 151, 410, 755
SmoI CTYRAG 4 cut(s) 128, 151, 410, 755
Sse9I AATT 5 cut(s) 31, 189, 268, 422, 619
SseBI AGGCCT 1 cut(s) 747
SsiI CCGC 2 cut(s) 10, 300
SspMI CTAG 6 cut(s) 242, 296, 387, 524, 572, 773
SstI GAGCTC 1 cut(s) 360
StuI AGGCCT 1 cut(s) 747
StyD4I CCNGG 4 cut(s) 324, 655, 731, 877
StyI CCWWGG 1 cut(s) 295
TaaI ACNGT 2 cut(s) 82, 469
TaqI TCGA 1 cut(s) 690
TasI AATT 5 cut(s) 31, 189, 268, 422, 619
TatI WGTACW 3 cut(s) 439, 759, 811
TauI GCSGC 1 cut(s) 12
TfiI GAWTC 2 cut(s) 366, 736
Tru1I TTAA 5 cut(s) 129, 152, 224, 651, 898
Tru9I TTAA 5 cut(s) 129, 152, 224, 651, 898
TscAI CASTG 2 cut(s) 85, 724
TseI GCWGC 1 cut(s) 234
TspDTI ATGAA 2 cut(s) 182, 498
TspGWI ACGGA 1 cut(s) 866
TspRI CASTG 2 cut(s) 85, 724
Tth111I GACNNNGTC 1 cut(s) 704
Van91I CCANNNNNTGG 1 cut(s) 331
Vha464I CTTAAG 2 cut(s) 128, 151
VpaK11BI GGWCC 2 cut(s) 64, 292
XapI RAATTY 1 cut(s) 31
XceI RCATGY 1 cut(s) 455
XmaJI CCTAGG 1 cut(s) 295
XmiI GTMKAC 1 cut(s) 780
XmnI GAANNNNTTC 1 cut(s) 340
XspI CTAG 6 cut(s) 242, 296, 387, 524, 572, 773
ZrmI AGTACT 2 cut(s) 441, 761
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.