Rh5BG010900

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0000540
Physical Location & Seq
Forward (+)
748997 .. 751765
2769 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG010900.1

Sequence Viewer

Length: 2616 bp
ATGGAGACGCTGTTAAGTCCTATGGTTCAAGTTGTACTGGACAAAGTATCTACGACAATGTTGAACAGAGCTCAAGTCATAGCAAAGTTTGGAGACGAGTTTGAGCAGCTGAAGGAAGGGTTAATAACTACGAGGGACCTACTCGCCGACTCGATGAATCTTCAGAGCAAGTATGACAACGTCAAGGGTGCCGTGAGCCAACTAAGGGAAGTAATTGATGAAGCTGACGATGTATTGACAGATTGCCTGGTTCGAGACATATACAAGGAAAATAAGTCCTCCCTACGTTTCTGGTTTCATGATCCGGGATTCTCAAGTCGAGTGGGCAAGCAGTTGGTAGATATCAATTCGAAAATGGATTCGGCACGTAGTATCTTGGGAAAACATTTGACAATTGCAAACTCTACAGCGCCTGATAGAACCCATCATGGTGAAAGCGATGCAAACCAGGGTGAAAGTGAAAGGGAATTCATGTCACGCAATTACGTCCCGCCTGTGACCTACGGATTGGATAAGGACATTAAAAGTTTAAAAGAGTGGATGTTTTGCAGTACCAAGGGGAAACTCGACTTTGTGGGCATTGTGGGGATGGGGGGGTTGGGCAAAACCACCATTGCCAAGAAGGTCTTTACTGATACAGAAGTCATATCTCACTTTAACAAGATGATCTGGGTGTCTGTTTCACAGAACTTTAGTATGAAGCGGATTCTGAAGTGCATGCTGGAAAAGGCAAATCTGCAAGCACCGGATGTTTCCGAATCTGATGACATGTTCACCAGCCTTAGACAAGGACTTAAAGATCAATCCTATCTCATTGTCATGGATGATGTGTGGCCTCATAAAAATTTAAATAGTTTTTGGACTAACCTCTGCGACTCACTACCAACAAAAATTGATAAAAGCAGCTGCATCATAATTACTACAAGGGATCGAGACGTTGCAAGAGTTATGGTGCACCAAGACTCACAAATACTTGAGCCCCCTACTCTGAATAACAGGGACAGCTGGTCTTTGTTCAGCGGATTTGCATTTCGATCAACCAAGGGAGAATGCCCGGATGAGAGCTTTGAGAAGGAAGGACAGGCTATCGTGAAGAGATGTGGTGGGCTTCCACTGGCACTCAAGACATTAGGAGGTTATTTGGCCAGGGAGAGTCGTCCTTCTGAGTGGACCAAGACAAAAGAAAAATTTCATTCATTGATGACTACCGATGGAAAAACAGAATCACTTATGCTTTGTCTACAAGTGAGCTATGATGAACTTCCAAACGATATGAAGCAATGCCTATTGTGTCTCTCCGTTTATCCTGAAGACTTTGAGATACACGCTGATCAGCTAATTCACTGGTGGTTTGGAGAGGGCCTCATCCGGGGCAAGAAAGGCGACTCTAAAACAGCACTAGAAGTGGGCTACGAAAATCTGGCAAAACTAGCCAATAGATGCCTATTGGAAGTTGTGGAGCGCCGAGGCTATGATGGAAGGATTATTAAGTTCAAAATACACGATATGGTACGGGAGTTAATAATCAGGATTGCAGAAAAAGAGAAATTTTGCAGCTTCAGCGAAGAATGTATGCAGATAGGGATTGAAGACTCTCGGTGGTTATGTCTCACTGAAGAGATGGCAGCTGAGGTTAAGAAGCCATGGATCAAAAGAAAGTTAAAGATGAGGTCATTTTTACTGATGTCAAGCCCTTCATTGGTTGACTTCAGCAAGAACTTTGTGTTGTTGGAGTCACTCAGGGTATTGGACTTGTCTTTCTCGAAGTTAGATGTGATTATTGTTGAAGATAGCAACACGGCCTCTTCGGGGATTTGTGTAGAGAAACTCTTCGACATGATCACTTCTCTGAAACGCCTAGCATGTCTAAACCTCAGTGGAGTTCAGAAACTATTAGAACTGCCATCTTCAATTGAGAAACTCCTTAACCTCCAGGTCTTGGTATTGAATGGATGCAGAAATTTAGGCAAAATACACGCCAAAATCACAAGTTTGAAGAGGCTTGTTGTCTTGGACATTGGAACATGTCCCCTCGAGCACCTGCCTCGGGACCTAGGGAGCCTCTCTTATCTTCAAGAACTCTCTGGATTTAAGGTGGTAAGTCGGGCTAAAACACGGGGTTGCACCCTTCTTGAGCTTGGAAAACTGGAGCAGCTAAGAGTTCTTCGAATTACAATAGGTGATGTGGCTGAAATCTCCGAAAAAGAAAAGAAGAGCCTATCAAGTCTCCAGAACCTCAAGGTTCTAGCTATTGATGCTGCAGATACATGCAATCAAGAAGATCTGGAAATGATAGATGAGCTGGAGTTGCCTCGAAAGATAGAGGAGCTGTTTCTCAGGAATTACCGCTCCCGAAAAATGCCAAACTGGTTTAGTCCCTTGAGGCTTCCTAAATTGCATTATCTTTCTATTGAGGATTGCGACGTTGAAAGCCTGAAGGACAACTGCTGTTGGAAAATTGAAGGTTTGTATTTAAAATTGTTAAATATGTTAGAGGTAGACTGGAATGACCTGAAGAGGGTTGTGCCAGCATTACGCTACATGGAGATTAGCAGATGTTCCAAATTGAAAAACTTTCCCATCGACGTTGTCAATCCAGGGGTTTGGAGAAAGTGA

Protein Analysis

871

Amino Acids

99.27

Weight (kDa)

6.92

Isoelectric Point (pI)

44.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 8 - 98 7.8e-09 Rx N-terminal domain
NB-ARC PF00931 172 - 347 1.6e-37 NB-ARC domain
WHD_DRP PF23559 434 - 508 3.7e-14 Disease resistance protein Winged helix domain
LRR_14 PF23598 572 - 805 6.8e-25 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 712 - 813 3.9e-10 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000540)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G50950 AT3G50950
fragaria_vesca FvH4_1g12710 FvH4_1g12710 FvH4_3g00820 FvH4_3g00820 FvH4_3g00820 FvH4_3g00820
malus_domestica MD02G1141100.v1.1 MD10G1333100.v1.1
prunus_persica Prupe.4G009300_v2.0.a1 Prupe.4G009300_v2.0.a1 Prupe.4G009300_v2.0.a1 Prupe.6G150700_v2.0.a1 Prupe.7G160100_v2.0.a1 Prupe.7G160100_v2.0.a1
pyrus_communis pycom02g11110 pycom10g28240 pycom10g28340 pycom10g28360 pycom10g28450
rosa_chinensis RchiOBHm_Chr2g0100711 RchiOBHm_Chr3g0485861 RchiOBHm_Chr4g0391451 RchiOBHm_Chr4g0391591 RchiOBHm_Chr4g0391601 RchiOBHm_Chr4g0391681 RchiOBHm_Chr5g0001141 RchiOBHm_Chr5g0001221
rosa_laevigata RLG00000017001
rosa_multiflora Rmu_co8260007.1_g000001 Rmu_co8260103.1_g000001 Rmu_co8519155.1_g000001 Rmu_sc0000060.1_g000050 Rmu_sc0000255.1_g000036 Rmu_sc0001174.1_g000023 Rmu_sc0001174.1_g000025 Rmu_sc0001659.1_g000009 Rmu_sc0001706.1_g000009 Rmu_sc0001706.1_g000024 Rmu_sc0001706.1_g000035 Rmu_sc0004189.1_g000018 Rmu_sc0004189.1_g000030 Rmu_sc0004862.1_g000002 Rmu_sc0004876.1_g000038 Rmu_sc0023741.1_g000004
rosa_roxburghii Rroxscaffold_1G00075100 Rroxscaffold_1G00075160 Rroxscaffold_1G00075210 Rroxscaffold_5G00337160 Rroxscaffold_5G00386590 Rroxscaffold_6G00396300
rosa_rugosa Rorug03G0336200 Rorug03G0336300 Rorug04G0389400
rosa_samantha Rh2AG140400 Rh2BG144100 Rh2CG145400 Rh2DG144900 Rh3AG271200 Rh4AG038000 Rh4AG038700 Rh4BG032000 Rh4BG032800 Rh4CG041100 Rh4CG041300 Rh4DG035500 Rh4DG035600 Rh5AG008800 Rh5AG009100 Rh5BG010900 Rh5BG011500 Rh5BG011600 Rh5BG012000 Rh5CG009400 Rh5CG010000 Rh5DG009800
rosa_wichuraiana Rw2G010940 Rw4G002950 Rw4G002990 Rw4G003000 Rw5G000880 Rw5G000950 Rw5G000960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2049
Acc36I ACCTGC 1 cut(s) 2049
AccB1I GGYRCC 1 cut(s) 188
AccB7I CCANNNNNTGG 1 cut(s) 1939
AccBSI CCGCTC 1 cut(s) 2349
AccI GTMKAC 2 cut(s) 1240, 2499
AciI CCGC 4 cut(s) 491, 703, 1020, 2347
AclWI GGATC 3 cut(s) 296, 936, 1655
AcoI YGGCCR 1 cut(s) 1143
AcsI RAATTY 5 cut(s) 467, 844, 1187, 1547, 1960
AcuI CTGAAG 9 cut(s) 131, 146, 731, 1329, 1543, 1635, 1693, 2456, 2534
AfaI GTAC 3 cut(s) 36, 553, 1512
AfiI CCNNNNNNNGG 7 cut(s) 205, 1369, 1699, 1809, 1939, 2047, 2518
AflIII ACRYGT 2 cut(s) 768, 2024
AhdI GACNNNNNGTC 1 cut(s) 1006
AjnI CCWGG 5 cut(s) 246, 447, 1145, 1932, 2596
Alw21I GWGCWC 3 cut(s) 73, 957, 2040
Alw26I GTCTC 6 cut(s) 87, 249, 927, 1298, 1613, 2231
Alw44I GTGCAC 1 cut(s) 953
AlwI GGATC 3 cut(s) 296, 936, 1655
AlwNI CAGNNNCTG 1 cut(s) 413
Ama87I CYCGRG 2 cut(s) 2033, 2046
AoxI GGCC 4 cut(s) 833, 1143, 1360, 1800
ApaLI GTGCAC 1 cut(s) 953
ApeKI GCWGC 7 cut(s) 106, 903, 906, 1554, 1625, 2152, 2258
ApoI RAATTY 5 cut(s) 467, 844, 1187, 1547, 1960
Asp700I GAANNNNTTC 2 cut(s) 1188, 1829
AspA2I CCTAGG 1 cut(s) 2053
AspLEI GCGC 2 cut(s) 412, 1464
AspS9I GGNCC 4 cut(s) 136, 1170, 1360, 2050
AsuC2I CCSGG 3 cut(s) 306, 1055, 1370
AsuHPI GGTGA 4 cut(s) 443, 464, 766, 2192
AsuII TTCGAA 2 cut(s) 350, 2167
AvaI CYCGRG 2 cut(s) 2033, 2046
AvaII GGWCC 3 cut(s) 136, 1170, 2050
AvrII CCTAGG 1 cut(s) 2053
BaeGI GKGCMC 1 cut(s) 957
BaeI ACNNNNGTAYC 4 cut(s) 627, 660, 1502, 1535
BalI TGGCCA 1 cut(s) 1145
BanI GGYRCC 1 cut(s) 188
BanII GRGCYC 2 cut(s) 73, 981
BbsI GAAGAC 2 cut(s) 1317, 1596
Bbv12I GWGCWC 3 cut(s) 73, 957, 2040
BbvCI CCTCAGC 1 cut(s) 1629
BbvI GCAGC 7 cut(s) 118, 893, 915, 1566, 1637, 2164, 2245
BccI CCATC 7 cut(s) 432, 583, 1205, 1469, 1615, 1912, 2588
BceAI ACGGC 2 cut(s) 176, 1815
BcgI CGANNNNNNTGC 2 cut(s) 2027, 2061
BciT130I CCWGG 5 cut(s) 248, 449, 1147, 1934, 2598
BclI TGATCA 2 cut(s) 1330, 1839
BcnI CCSGG 3 cut(s) 306, 1055, 1370
BcoDI GTCTC 6 cut(s) 87, 249, 927, 1298, 1613, 2231
BfaI CTAG 5 cut(s) 1400, 1430, 1859, 2054, 2246
BfmI CTRYAG 2 cut(s) 405, 2259
BfoI RGCGCY 2 cut(s) 413, 1465
BfuAI ACCTGC 1 cut(s) 2049
BglII AGATCT 1 cut(s) 2281
BisI GCNGC 7 cut(s) 107, 904, 907, 1555, 1626, 2153, 2259
BlnI CCTAGG 1 cut(s) 2053
BlsI GCNGC 7 cut(s) 108, 905, 908, 1556, 1627, 2154, 2260
Bme1390I CCNGG 8 cut(s) 248, 306, 449, 1055, 1147, 1370, 1934, 2598
Bme18I GGWCC 3 cut(s) 136, 1170, 2050
BmeRI GACNNNNNGTC 1 cut(s) 1006
BmeT110I CYCGRG 2 cut(s) 2033, 2046
BmgT120I GGNCC 4 cut(s) 136, 1170, 1360, 2050
BmiI GGNNCC 4 cut(s) 137, 190, 2051, 2060
BmrFI CCNGG 8 cut(s) 248, 306, 449, 1055, 1147, 1370, 1934, 2598
BmsI GCATC 5 cut(s) 430, 918, 1430, 1943, 2245
BpiI GAAGAC 2 cut(s) 1317, 1596
BplI GAGNNNNNCTC 2 cut(s) 1347, 1379
BpmI CTGGAG 4 cut(s) 1916, 2168, 2213, 2324
Bpu10I CCTNAGC 1 cut(s) 1629
Bpu14I TTCGAA 2 cut(s) 350, 2167
BpuEI CTTGAG 7 cut(s) 57, 298, 995, 1106, 2153, 2222, 2401
BpuMI CCSGG 3 cut(s) 306, 1055, 1370
BsaAI YACGTR 1 cut(s) 368
BsaWI WCCGGW 1 cut(s) 745
BsaXI ACNNNNNCTCC 1 cut(s) 26
Bsc4I CCNNNNNNNGG 7 cut(s) 205, 1369, 1699, 1809, 1939, 2047, 2518
Bse1I ACTGG 6 cut(s) 42, 1119, 1349, 2151, 2372, 2507
Bse3DI GCAATG 2 cut(s) 612, 1286
BseBI CCWGG 5 cut(s) 248, 449, 1147, 1934, 2598
BseGI GGATG 7 cut(s) 546, 594, 754, 829, 1063, 1365, 1958
BseLI CCNNNNNNNGG 7 cut(s) 205, 1369, 1699, 1809, 1939, 2047, 2518
BseMI GCAATG 2 cut(s) 612, 1286
BseMII CTCAG 5 cut(s) 1155, 1620, 1753, 1888, 2350
BseNI ACTGG 6 cut(s) 42, 1119, 1349, 2151, 2372, 2507
BseRI GAGGAG 1 cut(s) 2339
BseSI GKGCMC 1 cut(s) 957
BseXI GCAGC 7 cut(s) 118, 893, 915, 1566, 1637, 2164, 2245
BshFI GGCC 4 cut(s) 835, 1145, 1362, 1802
BshNI GGYRCC 1 cut(s) 188
BsiHKAI GWGCWC 3 cut(s) 73, 957, 2040
BsiHKCI CYCGRG 2 cut(s) 2033, 2046
BsiSI CCGG 4 cut(s) 305, 746, 1055, 1369
BslFI GGGAC 6 cut(s) 149, 473, 1013, 2013, 2063, 2361
BslI CCNNNNNNNGG 7 cut(s) 205, 1369, 1699, 1809, 1939, 2047, 2518
BsmAI GTCTC 6 cut(s) 87, 249, 927, 1298, 1613, 2231
BsmBI CGTCTC 2 cut(s) 87, 927
BsmFI GGGAC 6 cut(s) 149, 473, 1013, 2013, 2063, 2361
BsmI GAATGC 1 cut(s) 1055
BsnI GGCC 4 cut(s) 835, 1145, 1362, 1802
BsoBI CYCGRG 2 cut(s) 2033, 2046
Bsp119I TTCGAA 2 cut(s) 350, 2167
Bsp1286I GDGCHC 4 cut(s) 73, 957, 981, 2040
Bsp143I GATC 9 cut(s) 301, 666, 799, 928, 1034, 1330, 1647, 1839, 2281
Bsp19I CCATGG 1 cut(s) 1643
BspACI CCGC 4 cut(s) 491, 703, 1020, 2347
BspANI GGCC 4 cut(s) 835, 1145, 1362, 1802
BspCNI CTCAG 5 cut(s) 1156, 1621, 1752, 1887, 2349
BspHI TCATGA 1 cut(s) 298
BspLI GGNNCC 4 cut(s) 137, 190, 2051, 2060
BspMAI CTGCAG 1 cut(s) 2263
BspMI ACCTGC 1 cut(s) 2049
BspPI GGATC 3 cut(s) 296, 936, 1655
BspQI GCTCTTC 1 cut(s) 2207
BspT104I TTCGAA 2 cut(s) 350, 2167
BspT107I GGYRCC 1 cut(s) 188
BsrBI CCGCTC 1 cut(s) 2349
BsrDI GCAATG 2 cut(s) 612, 1286
BsrI ACTGG 6 cut(s) 42, 1119, 1349, 2151, 2372, 2507
BssMI GATC 9 cut(s) 301, 666, 799, 928, 1034, 1330, 1647, 1839, 2281
BssT1I CCWWGG 4 cut(s) 555, 1041, 1643, 2053
Bst2UI CCWGG 5 cut(s) 248, 449, 1147, 1934, 2598
Bst6I CTCTTC 7 cut(s) 1088, 1611, 1810, 1835, 1991, 2207, 2510
BstBAI YACGTR 1 cut(s) 368
BstBI TTCGAA 2 cut(s) 350, 2167
BstC8I GCNNGC 4 cut(s) 329, 719, 741, 2529
BstDEI CTNAG 8 cut(s) 203, 782, 1164, 1629, 1739, 1874, 2156, 2336
BstDSI CCRYGG 1 cut(s) 1643
BstF5I GGATG 7 cut(s) 546, 594, 754, 829, 1063, 1365, 1958
BstH2I RGCGCY 2 cut(s) 413, 1465
BstHHI GCGC 2 cut(s) 412, 1464
BstKTI GATC 9 cut(s) 304, 669, 802, 931, 1037, 1333, 1650, 1842, 2284
BstMAI GTCTC 6 cut(s) 87, 249, 927, 1298, 1613, 2231
BstMBI GATC 9 cut(s) 301, 666, 799, 928, 1034, 1330, 1647, 1839, 2281
BstMWI GCNNNNNNNGC 5 cut(s) 1380, 1430, 1560, 2255, 2308
BstNI CCWGG 5 cut(s) 248, 449, 1147, 1934, 2598
BstNSI RCATGY 5 cut(s) 721, 772, 1866, 2028, 2271
BstSCI CCNGG 8 cut(s) 246, 304, 447, 1053, 1145, 1368, 1932, 2596
BstSFI CTRYAG 2 cut(s) 405, 2259
BstSLI GKGCMC 1 cut(s) 957
BstV1I GCAGC 7 cut(s) 118, 893, 915, 1566, 1637, 2164, 2245
BstV2I GAAGAC 2 cut(s) 1317, 1596
BstX2I RGATCY 1 cut(s) 2281
BstXI CCANNNNNNTGG 1 cut(s) 2604
BstYI RGATCY 1 cut(s) 2281
BsuRI GGCC 4 cut(s) 835, 1145, 1362, 1802
BtgI CCRYGG 1 cut(s) 1643
BtgZI GCGATG 1 cut(s) 453
BtsCI GGATG 7 cut(s) 546, 594, 754, 829, 1063, 1365, 1958
BtsIMutI CAGTG 4 cut(s) 1112, 1342, 1611, 1882
BveI ACCTGC 1 cut(s) 2049
Cac8I GCNNGC 4 cut(s) 329, 719, 741, 2529
CaiI CAGNNNCTG 1 cut(s) 413
CciI TCATGA 1 cut(s) 298
CfoI GCGC 2 cut(s) 412, 1464
Cfr13I GGNCC 4 cut(s) 136, 1170, 1360, 2050
CseI GACGC 1 cut(s) 16
Csp6I GTAC 3 cut(s) 35, 552, 1511
CspCI CAANNNNNGTGG 2 cut(s) 303, 338
CviQI GTAC 3 cut(s) 35, 552, 1511
DdeI CTNAG 8 cut(s) 203, 782, 1164, 1629, 1739, 1874, 2156, 2336
DpnI GATC 9 cut(s) 303, 668, 801, 930, 1036, 1332, 1649, 1841, 2283
DpnII GATC 9 cut(s) 301, 666, 799, 928, 1034, 1330, 1647, 1839, 2281
DraI TTTAAA 3 cut(s) 531, 849, 2475
DriI GACNNNNNGTC 1 cut(s) 1006
EaeI YGGCCR 1 cut(s) 1143
Eam1104I CTCTTC 7 cut(s) 1088, 1611, 1810, 1835, 1991, 2207, 2510
Eam1105I GACNNNNNGTC 1 cut(s) 1006
EarI CTCTTC 7 cut(s) 1088, 1611, 1810, 1835, 1991, 2207, 2510
Ecl136II GAGCTC 1 cut(s) 71
Eco130I CCWWGG 4 cut(s) 555, 1041, 1643, 2053
Eco24I GRGCYC 2 cut(s) 73, 981
Eco32I GATATC 1 cut(s) 343
Eco47I GGWCC 3 cut(s) 136, 1170, 2050
Eco53kI GAGCTC 1 cut(s) 71
Eco57I CTGAAG 9 cut(s) 131, 146, 731, 1329, 1543, 1635, 1693, 2456, 2534
Eco88I CYCGRG 2 cut(s) 2033, 2046
EcoICRI GAGCTC 1 cut(s) 71
EcoO109I RGGNCCY 3 cut(s) 136, 1360, 2050
EcoRI GAATTC 1 cut(s) 467
EcoRII CCWGG 5 cut(s) 246, 447, 1145, 1932, 2596
EcoRV GATATC 1 cut(s) 343
EcoT14I CCWWGG 4 cut(s) 555, 1041, 1643, 2053
EcoT38I GRGCYC 2 cut(s) 73, 981
ErhI CCWWGG 4 cut(s) 555, 1041, 1643, 2053
Esp3I CGTCTC 2 cut(s) 87, 927
FalI AAGNNNNNCTT 2 cut(s) 611, 643
FaqI GGGAC 6 cut(s) 149, 473, 1013, 2013, 2063, 2361
FauI CCCGC 1 cut(s) 498
FbaI TGATCA 2 cut(s) 1330, 1839
FblI GTMKAC 2 cut(s) 1240, 2499
Fnu4HI GCNGC 7 cut(s) 107, 904, 907, 1555, 1626, 2153, 2259
FokI GGATG 7 cut(s) 553, 601, 761, 836, 1070, 1352, 1965
FriOI GRGCYC 2 cut(s) 73, 981
Fsp4HI GCNGC 7 cut(s) 107, 904, 907, 1555, 1626, 2153, 2259
FspBI CTAG 5 cut(s) 1400, 1430, 1859, 2054, 2246
GlaI GCGC 2 cut(s) 411, 1463
GluI GCNGC 7 cut(s) 107, 904, 907, 1555, 1626, 2153, 2259
GsuI CTGGAG 4 cut(s) 1916, 2168, 2213, 2324
HaeII RGCGCY 2 cut(s) 413, 1465
HaeIII GGCC 4 cut(s) 835, 1145, 1362, 1802
HapII CCGG 4 cut(s) 305, 746, 1055, 1369
HgaI GACGC 1 cut(s) 16
HhaI GCGC 2 cut(s) 412, 1464
Hin6I GCGC 2 cut(s) 410, 1462
HinP1I GCGC 2 cut(s) 410, 1462
HincII GTYRAC 1 cut(s) 1705
HindII GTYRAC 1 cut(s) 1705
HpaII CCGG 4 cut(s) 305, 746, 1055, 1369
HphI GGTGA 4 cut(s) 443, 464, 766, 2192
Hpy166II GTNNAC 6 cut(s) 774, 955, 1170, 1241, 1705, 2500
Hpy188I TCNGA 9 cut(s) 165, 711, 757, 763, 990, 1165, 1851, 1887, 2200
Hpy8I GTNNAC 6 cut(s) 774, 955, 1170, 1241, 1705, 2500
Hpy99I CGWCG 2 cut(s) 2426, 2588
HpyCH4IV ACGT 7 cut(s) 180, 286, 367, 486, 936, 2424, 2586
HpyF10VI GCNNNNNNNGC 5 cut(s) 1380, 1430, 1560, 2255, 2308
HpyF3I CTNAG 8 cut(s) 203, 782, 1164, 1629, 1739, 1874, 2156, 2336
HpySE526I ACGT 7 cut(s) 180, 286, 367, 486, 936, 2424, 2586
HspAI GCGC 2 cut(s) 410, 1462
Ksp22I TGATCA 2 cut(s) 1330, 1839
Kzo9I GATC 9 cut(s) 301, 666, 799, 928, 1034, 1330, 1647, 1839, 2281
LguI GCTCTTC 1 cut(s) 2207
LmnI GCTCC 5 cut(s) 1459, 2058, 2149, 2326, 2354
Lsp1109I GCAGC 7 cut(s) 118, 893, 915, 1566, 1637, 2164, 2245
LweI GCATC 5 cut(s) 430, 918, 1430, 1943, 2245
MaeI CTAG 5 cut(s) 1400, 1430, 1859, 2054, 2246
MaeII ACGT 7 cut(s) 180, 286, 367, 486, 936, 2424, 2586
MaeIII GTNAC 3 cut(s) 474, 496, 1734
MalI GATC 9 cut(s) 303, 668, 801, 930, 1036, 1332, 1649, 1841, 2283
MbiI CCGCTC 1 cut(s) 2349
MboI GATC 9 cut(s) 301, 666, 799, 928, 1034, 1330, 1647, 1839, 2281
MfeI CAATTG 2 cut(s) 393, 1911
MflI RGATCY 1 cut(s) 2281
MhlI GDGCHC 4 cut(s) 73, 957, 981, 2040
MlsI TGGCCA 1 cut(s) 1145
MluNI TGGCCA 1 cut(s) 1145
MlyI GAGTC 7 cut(s) 143, 869, 956, 1162, 1379, 1586, 1742
MmeI TCCRAC 2 cut(s) 1710, 2432
Mox20I TGGCCA 1 cut(s) 1145
MroXI GAANNNNTTC 2 cut(s) 1188, 1829
MscI TGGCCA 1 cut(s) 1145
MslI CAYNNNNRTG 2 cut(s) 429, 818
Msp20I TGGCCA 1 cut(s) 1145
MspA1I CMGCKG 5 cut(s) 109, 906, 1005, 1020, 1628
MspI CCGG 4 cut(s) 305, 746, 1055, 1369
MspR9I CCNGG 8 cut(s) 248, 306, 449, 1055, 1147, 1370, 1934, 2598
MunI CAATTG 2 cut(s) 393, 1911
Mva1269I GAATGC 1 cut(s) 1055
MvaI CCWGG 5 cut(s) 248, 449, 1147, 1934, 2598
MwoI GCNNNNNNNGC 5 cut(s) 1380, 1430, 1560, 2255, 2308
NciI CCSGG 3 cut(s) 306, 1055, 1370
NcoI CCATGG 1 cut(s) 1643
NdeII GATC 9 cut(s) 301, 666, 799, 928, 1034, 1330, 1647, 1839, 2281
NlaIV GGNNCC 4 cut(s) 137, 190, 2051, 2060
NmeAIII GCCGAG 1 cut(s) 1490
NmuCI GTSAC 3 cut(s) 474, 496, 1734
NspI RCATGY 5 cut(s) 721, 772, 1866, 2028, 2271
NspV TTCGAA 2 cut(s) 350, 2167
PaeI GCATGC 1 cut(s) 721
PaeR7I CTCGAG 1 cut(s) 2033
PagI TCATGA 1 cut(s) 298
PaqCI CACCTGC 1 cut(s) 2049
PciI ACATGT 2 cut(s) 768, 2024
PciSI GCTCTTC 1 cut(s) 2207
PctI GAATGC 1 cut(s) 1055
PdmI GAANNNNTTC 2 cut(s) 1188, 1829
PfeI GAWTC 6 cut(s) 157, 309, 359, 706, 758, 1223
PflFI GACNNNGTC 2 cut(s) 179, 2588
PflMI CCANNNNNTGG 1 cut(s) 1939
PfoI TCCNGGA 1 cut(s) 304
PkrI GCNGC 7 cut(s) 108, 905, 908, 1556, 1627, 2154, 2260
PleI GAGTC 7 cut(s) 143, 869, 956, 1161, 1379, 1586, 1741
PpsI GAGTC 7 cut(s) 143, 869, 956, 1161, 1379, 1586, 1741
Ppu21I YACGTR 1 cut(s) 368
PpuMI RGGWCCY 2 cut(s) 136, 2050
PscI ACATGT 2 cut(s) 768, 2024
Psp124BI GAGCTC 1 cut(s) 73
Psp5II RGGWCCY 2 cut(s) 136, 2050
Psp6I CCWGG 5 cut(s) 246, 447, 1145, 1932, 2596
PspGI CCWGG 5 cut(s) 246, 447, 1145, 1932, 2596
PspN4I GGNNCC 4 cut(s) 137, 190, 2051, 2060
PspPI GGNCC 4 cut(s) 136, 1170, 1360, 2050
PspPPI RGGWCCY 2 cut(s) 136, 2050
PspXI VCTCGAGB 1 cut(s) 2033
PstI CTGCAG 1 cut(s) 2263
PstNI CAGNNNCTG 1 cut(s) 413
PsuI RGATCY 1 cut(s) 2281
PsyI GACNNNGTC 2 cut(s) 179, 2588
PvuII CAGCTG 4 cut(s) 109, 906, 1005, 1628
RsaI GTAC 3 cut(s) 36, 553, 1512
RsaNI GTAC 3 cut(s) 35, 552, 1511
RseI CAYNNNNRTG 2 cut(s) 429, 818
SacI GAGCTC 1 cut(s) 73
SapI GCTCTTC 1 cut(s) 2207
SatI GCNGC 7 cut(s) 107, 904, 907, 1555, 1626, 2153, 2259
Sau3AI GATC 9 cut(s) 301, 666, 799, 928, 1034, 1330, 1647, 1839, 2281
Sau96I GGNCC 4 cut(s) 136, 1170, 1360, 2050
SchI GAGTC 7 cut(s) 143, 869, 956, 1162, 1379, 1586, 1742
ScrFI CCNGG 8 cut(s) 248, 306, 449, 1055, 1147, 1370, 1934, 2598
SduI GDGCHC 4 cut(s) 73, 957, 981, 2040
SfaNI GCATC 5 cut(s) 430, 918, 1430, 1943, 2245
SfcI CTRYAG 2 cut(s) 405, 2259
Sfr274I CTCGAG 1 cut(s) 2033
SfuI TTCGAA 2 cut(s) 350, 2167
SinI GGWCC 3 cut(s) 136, 1170, 2050
SlaI CTCGAG 1 cut(s) 2033
SmiI ATTTAAAT 1 cut(s) 849
SmiMI CAYNNNNRTG 2 cut(s) 429, 818
SmlI CTYRAG 8 cut(s) 72, 313, 974, 1121, 2033, 2132, 2237, 2380
SmoI CTYRAG 8 cut(s) 72, 313, 974, 1121, 2033, 2132, 2237, 2380
SphI GCATGC 1 cut(s) 721
SsiI CCGC 4 cut(s) 491, 703, 1020, 2347
SspMI CTAG 5 cut(s) 1400, 1430, 1859, 2054, 2246
SstI GAGCTC 1 cut(s) 73
StyD4I CCNGG 8 cut(s) 246, 304, 447, 1053, 1145, 1368, 1932, 2596
StyI CCWWGG 4 cut(s) 555, 1041, 1643, 2053
SwaI ATTTAAAT 1 cut(s) 849
TaiI ACGT 7 cut(s) 183, 289, 370, 489, 939, 2427, 2589
TatI WGTACW 1 cut(s) 34
TfiI GAWTC 6 cut(s) 157, 309, 359, 706, 758, 1223
TscAI CASTG 4 cut(s) 1119, 1349, 1618, 1882
TseFI GTSAC 3 cut(s) 474, 496, 1734
TseI GCWGC 7 cut(s) 106, 903, 906, 1554, 1625, 2152, 2258
Tsp45I GTSAC 3 cut(s) 474, 496, 1734
TspGWI ACGGA 2 cut(s) 519, 1288
TspRI CASTG 4 cut(s) 1119, 1349, 1618, 1882
Tth111I GACNNNGTC 2 cut(s) 179, 2588
Van91I CCANNNNNTGG 1 cut(s) 1939
VneI GTGCAC 1 cut(s) 953
VpaK11BI GGWCC 3 cut(s) 136, 1170, 2050
XapI RAATTY 5 cut(s) 467, 844, 1187, 1547, 1960
XceI RCATGY 5 cut(s) 721, 772, 1866, 2028, 2271
XhoI CTCGAG 1 cut(s) 2033
XmaJI CCTAGG 1 cut(s) 2053
XmiI GTMKAC 2 cut(s) 1240, 2499
XmnI GAANNNNTTC 2 cut(s) 1188, 1829
XspI CTAG 5 cut(s) 1400, 1430, 1859, 2054, 2246
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.