Rh5AG009100

NB-ARC domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0000540
Physical Location & Seq
Reverse (-)
628345 .. 630864
2520 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG009100.1

Sequence Viewer

Length: 2520 bp
ATGTCGTTAATAGTGGAGGCTATGGTCCAGACTTTAACGGAGCAAGTGTTCACAGCTCTAATGAACCAGGCTGAATTTGCACTTGACTTCAGCGGCCAGTTTGAGCAATTGAAGACAGGGCTTGATCTCACCAAGGCCTTACTTGCAGACACGGAGAGTCTCAACCACAAGAACAACATTGTCAAGGCAGCATTGTTTGAGCTAAGGGAAGTCATCTACGAAGCCGACAATGTACTCACTGATTGCTTGGTTAGAGATGAATACAGAAAGGACTGGTCTTGCTCCGGCTCATTGTTGCACGATCCGTTCTTCTTGCATCGGACAGGCAAGAAGTTGAGAGACATAAATTTGCGTATGAAGCAGATAGAGAAGACATTGGGCCGCTTTTTGAGGGCACCGGACAGCATCCATAGAGCAGATGCATACCAAGTTAGAGTAATGGTGTCACAGGACTGGAATCCAACTGAGATAATTGGGTTGGATAGCGATGTGGAGAAGATTAAGGGTTGGATATTTTTTGACACCAGCAAGGTACTTCACCACGTAGGCATTGTGGGAATGGGTGGCTTGGGCAAAACCACCATTGCTCAAAAGATATTTCATGATCTAGAAGTAGTAGGCCACTTTCACAAGCTGATATGGGTGTGTGTTTCGCAATCATTCAGCGCTGAAAGGATTATGAGGAGCATCCTAGAAGGTCTAGGAGAAAATGTCTCTGGAGCCGGTGTTACTCAGATTTTGAGCAGAATTCAGCGAGTACTTAAAGATAAGAGCTGTCTGATTGTCATGGATGATGTATGGAATCACACAGAATTTGATTGGTGGACCAACCTCTGCTCCGTTATACCTAAACAAAGCAGCTGCATCATAGTTACAACTAGAAATGAAGAAGCTGCAATTGGTATGGGAGTTGAAAGCTCACGAATCCACCGTCCGAAGACTCTTAATGATGAGGAAAGCTGGTGTTTATTCTCCAAATTTGCATTTCCTACAAGCAAGGGATTGTGCCCGGAGGACAGGCTGGAGAAATTAGGAAAAGAACTCTTGAAGAAGTGTGGTGGACTTCCACTCGCAATCAAGACTATAGGATCCTTGTTGGCATCAAAGATCGACTCCCCTTCTCAGTGGAGAAGAGTTCTGGAGAGCTTTCATGCTTTGACAACCGAGGGAAAAACTAGCTCAGTTATGGCCTCTTTGCGATTGAGCTATGATGAACTCCCGCCTGGTCTGAAGCAATGCTTGTTGTGTTTCTCCATCTATCCTGAAGACTATGAGACACGTGCTGATCAGTTAATCCACTGGTGGATTGGAGAAGGCCTCGTGCAAAGTAAGGGCTCAAGAACAGCAGTAGAAGTAGGATATGAGTATCTAGGGGAACTAATCAGCAGATGTTTGGTTGAAATTGTGCAGAGGAGAGGATTTGATGGGAGAGTCTACAAGTGCAAGATACACGATATGGTACGGGAACTGATAATAATGATTGCAGAGGAAGAGGAATTTTGCGGCTTCGACGAACGAGGTAGACAGGAATTGACAGCAAAGACTAGGTGGTTATGCTTTATTGATGAAATGGAGGAGAAGTCCTTGAAACGTAGTTCAAAGCTCAGGGCACTGTTGCTGATGTCAAGCCGCAAATTTGACTTTGAAAGGAATTCGGGGTTTCTGCGATCCCTCAGAATGTTGGATTTGTCAAACTGCACTGTGGACGAGCATTGTGTGGAGGATCTCTTCAGCTGGGTCAGCTCCCTTAAGCGCTTGGCATCTCTGAACTTAAGTGGTATTCAAGCCCTGAAAGAAGTGCCCTCTTCAATTCATAAACTCCTGAACCTCCAACTTTTGCTTTTAACAGGCTGCAGCAATCTAGTAAAAATACACCCTTCCATCACTAATTTGAAGAAGCTGATTATTTTGGACCTGGGCGGGTGTCCAATCCAATATTTGCCTCAGGGATTGGGAAGGCTTTCTTATCTTCAAGAGCTCTCTGGATTCAAGGTTGTGAGTCAGCCTAGAAAGCCAAGTTTCCGGCTTCTTGAGATTAGGGAATTGATCAACCTGAGAGTGTTGCGAATACATATAAGCAGTACCACAGTTATCGCAGATAATGAACTTGATGTCCTCTCTCAGCTTAGAAGGCTCAATGTTCTAGCTATTGATGCAGAAGATTGTAGAAATAACAATGTTTTGGAAATGCTAGAAGGGCTGACCCCTCCTCCAAGCCTCCAAGAGCTATATCTTAGGAATTACAAGAAGGAAACTCTGCCCAACTGGGTTAATCCTGGACAACTTTCACGATTGCAGTATCTTTGCATCGAGAATGGAGACCTTGTCAAGCTAAGCAGTGGTCAAACCACCTGGAATCTTGAAGGCCTGTGTCTCAAGTACCTGATGAGGCTAGAGGTAGACTGGAAGGACTTGGAGAAGGATATGCCTGTACTTCACTACATGGAGATCAGTCATTGTTACAACTTGACGGATTTCCCTTGTTCGGTTATGGAACCTGGGGTCTGGAGAAAGCATTAA

Protein Analysis

839

Amino Acids

95.76

Weight (kDa)

6.52

Isoelectric Point (pI)

43.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 8 - 94 4.2e-12 Rx N-terminal domain
NB-ARC PF00931 162 - 332 2.9e-39 NB-ARC domain
AAA_22 PF13401 184 - 266 5.6e-06 AAA domain
WHD_DRP PF23559 419 - 491 3.4e-19 Disease resistance protein Winged helix domain
LRR_14 PF23598 551 - 771 1.1e-27 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 692 - 780 1.1e-08 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000540)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G50950 AT3G50950
fragaria_vesca FvH4_1g12710 FvH4_1g12710 FvH4_3g00820 FvH4_3g00820 FvH4_3g00820 FvH4_3g00820
malus_domestica MD02G1141100.v1.1 MD10G1333100.v1.1
prunus_persica Prupe.4G009300_v2.0.a1 Prupe.4G009300_v2.0.a1 Prupe.4G009300_v2.0.a1 Prupe.6G150700_v2.0.a1 Prupe.7G160100_v2.0.a1 Prupe.7G160100_v2.0.a1
pyrus_communis pycom02g11110 pycom10g28240 pycom10g28340 pycom10g28360 pycom10g28450
rosa_chinensis RchiOBHm_Chr2g0100711 RchiOBHm_Chr3g0485861 RchiOBHm_Chr4g0391451 RchiOBHm_Chr4g0391591 RchiOBHm_Chr4g0391601 RchiOBHm_Chr4g0391681 RchiOBHm_Chr5g0001141 RchiOBHm_Chr5g0001221
rosa_laevigata RLG00000017001
rosa_multiflora Rmu_co8260007.1_g000001 Rmu_co8260103.1_g000001 Rmu_co8519155.1_g000001 Rmu_sc0000060.1_g000050 Rmu_sc0000255.1_g000036 Rmu_sc0001174.1_g000023 Rmu_sc0001174.1_g000025 Rmu_sc0001659.1_g000009 Rmu_sc0001706.1_g000009 Rmu_sc0001706.1_g000024 Rmu_sc0001706.1_g000035 Rmu_sc0004189.1_g000018 Rmu_sc0004189.1_g000030 Rmu_sc0004862.1_g000002 Rmu_sc0004876.1_g000038 Rmu_sc0023741.1_g000004
rosa_roxburghii Rroxscaffold_1G00075100 Rroxscaffold_1G00075160 Rroxscaffold_1G00075210 Rroxscaffold_5G00337160 Rroxscaffold_5G00386590 Rroxscaffold_6G00396300
rosa_rugosa Rorug03G0336200 Rorug03G0336300 Rorug04G0389400
rosa_samantha Rh2AG140400 Rh2BG144100 Rh2CG145400 Rh2DG144900 Rh3AG271200 Rh4AG038000 Rh4AG038700 Rh4BG032000 Rh4BG032800 Rh4CG041100 Rh4CG041300 Rh4DG035500 Rh4DG035600 Rh5AG008800 Rh5AG009100 Rh5BG010900 Rh5BG011500 Rh5BG011600 Rh5BG012000 Rh5CG009400 Rh5CG010000 Rh5DG009800
rosa_wichuraiana Rw2G010940 Rw4G002950 Rw4G002990 Rw4G003000 Rw5G000880 Rw5G000950 Rw5G000960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 394
AccI GTMKAC 3 cut(s) 1434, 1522, 2400
AciI CCGC 6 cut(s) 93, 382, 1220, 1503, 1630, 1920
AclWI GGATC 5 cut(s) 296, 1083, 1096, 1662, 1731
AcoI YGGCCR 1 cut(s) 94
AcsI RAATTY 8 cut(s) 74, 346, 747, 812, 977, 1496, 1634, 1651
AcuI CTGAAG 4 cut(s) 73, 1250, 1284, 1714
AcvI CACGTG 1 cut(s) 1280
AfaI GTAC 7 cut(s) 234, 534, 759, 1461, 2083, 2381, 2433
AfeI AGCGCT 2 cut(s) 667, 1754
AfiI CCNNNNNNNGG 1 cut(s) 2485
AflII CTTAAG 2 cut(s) 1748, 1771
AflIII ACRYGT 1 cut(s) 1277
AjnI CCWGG 6 cut(s) 66, 1222, 1914, 2275, 2351, 2497
AjuI GAANNNNNNNTTGG 2 cut(s) 882, 914
AloI GAACNNNNNNTCC 4 cut(s) 32, 64, 2097, 2129
Alw21I GWGCWC 1 cut(s) 1980
Alw26I GTCTC 6 cut(s) 164, 333, 718, 1268, 2313, 2378
AlwI GGATC 5 cut(s) 296, 1083, 1096, 1662, 1731
Aor51HI AGCGCT 2 cut(s) 667, 1754
AoxI GGCC 7 cut(s) 94, 135, 379, 619, 1188, 1315, 2365
ApeKI GCWGC 6 cut(s) 188, 858, 861, 893, 1851, 1854
ApoI RAATTY 8 cut(s) 74, 346, 747, 812, 977, 1496, 1634, 1651
ArsI GACNNNNNNTTYG 2 cut(s) 916, 948
Asp700I GAANNNNTTC 1 cut(s) 1960
AspLEI GCGC 2 cut(s) 668, 1755
AspS9I GGNCC 4 cut(s) 25, 379, 825, 1912
AsuC2I CCSGG 1 cut(s) 1010
AsuHPI GGTGA 2 cut(s) 121, 530
AvaII GGWCC 3 cut(s) 25, 825, 1912
AxyI CCTNAGG 1 cut(s) 1944
BaeGI GKGCMC 4 cut(s) 397, 1010, 1612, 1803
BamHI GGATCC 1 cut(s) 1088
BanI GGYRCC 1 cut(s) 394
BanII GRGCYC 2 cut(s) 1337, 1980
BauI CACGAG 1 cut(s) 1319
BbrPI CACGTG 1 cut(s) 1280
BbsI GAAGAC 4 cut(s) 119, 377, 944, 1272
Bbv12I GWGCWC 1 cut(s) 1980
BbvI GCAGC 6 cut(s) 200, 848, 870, 880, 1838, 1866
BccI CCATC 3 cut(s) 1262, 1418, 1889
BciT130I CCWGG 6 cut(s) 68, 1224, 1916, 2277, 2353, 2499
BclI TGATCA 2 cut(s) 1285, 2046
BcnI CCSGG 1 cut(s) 1010
BcoDI GTCTC 6 cut(s) 164, 333, 718, 1268, 2313, 2378
BfmI CTRYAG 2 cut(s) 1083, 1852
BfoI RGCGCY 2 cut(s) 669, 1756
BfrI CTTAAG 2 cut(s) 1748, 1771
BlpI GCTNAGC 1 cut(s) 2333
BmcAI AGTACT 1 cut(s) 759
Bme1390I CCNGG 7 cut(s) 68, 1010, 1224, 1916, 2277, 2353, 2499
Bme18I GGWCC 3 cut(s) 25, 825, 1912
BmgT120I GGNCC 4 cut(s) 25, 379, 825, 1912
BmiI GGNNCC 4 cut(s) 396, 721, 1090, 2496
BmrFI CCNGG 7 cut(s) 68, 1010, 1224, 1916, 2277, 2353, 2499
BmrI ACTGGG 1 cut(s) 2275
BmsI GCATC 9 cut(s) 325, 409, 414, 696, 873, 1109, 1769, 2143, 2316
BmuI ACTGGG 1 cut(s) 2275
BpiI GAAGAC 4 cut(s) 119, 377, 944, 1272
BplI GAGNNNNNCTC 2 cut(s) 1302, 1334
BpmI CTGGAG 3 cut(s) 738, 1043, 1160
Bpu10I CCTNAGC 2 cut(s) 203, 1604
Bpu1102I GCTNAGC 1 cut(s) 2333
BpuEI CTTGAG 3 cut(s) 1321, 2051, 2360
BpuMI CCSGG 1 cut(s) 1010
BsaAI YACGTR 2 cut(s) 544, 1280
BsaI GGTCTC 1 cut(s) 2313
BsaJI CCNNGG 4 cut(s) 132, 1164, 1915, 2498
BsaWI WCCGGW 1 cut(s) 397
Bsc4I CCNNNNNNNGG 1 cut(s) 2485
Bse118I RCCGGY 1 cut(s) 722
Bse1I ACTGG 6 cut(s) 97, 278, 458, 1304, 2270, 2408
Bse21I CCTNAGG 1 cut(s) 1944
Bse3DI GCAATG 2 cut(s) 582, 1241
BseBI CCWGG 6 cut(s) 68, 1224, 1916, 2277, 2353, 2499
BseDI CCNNGG 4 cut(s) 132, 1164, 1915, 2498
BseGI GGATG 3 cut(s) 405, 687, 796
BseLI CCNNNNNNNGG 1 cut(s) 2485
BseMI GCAATG 2 cut(s) 582, 1241
BseMII CTCAG 9 cut(s) 456, 746, 1136, 1194, 1618, 1687, 1958, 2045, 2135
BseNI ACTGG 6 cut(s) 97, 278, 458, 1304, 2270, 2408
BseRI GAGGAG 4 cut(s) 697, 1426, 1589, 2199
BseSI GKGCMC 4 cut(s) 397, 1010, 1612, 1803
BseXI GCAGC 6 cut(s) 200, 848, 870, 880, 1838, 1866
BseYI CCCAGC 1 cut(s) 1734
BsgI GTGCAG 2 cut(s) 1427, 1681
BshFI GGCC 7 cut(s) 96, 137, 381, 621, 1190, 1317, 2367
BshNI GGYRCC 1 cut(s) 394
BsiHKAI GWGCWC 1 cut(s) 1980
BsiSI CCGG 5 cut(s) 285, 398, 723, 1010, 2023
BslI CCNNNNNNNGG 1 cut(s) 2485
BsmAI GTCTC 6 cut(s) 164, 333, 718, 1268, 2313, 2378
BsnI GGCC 7 cut(s) 96, 137, 381, 621, 1190, 1317, 2367
Bso31I GGTCTC 1 cut(s) 2313
Bsp1286I GDGCHC 6 cut(s) 397, 1010, 1337, 1612, 1803, 1980
Bsp1720I GCTNAGC 1 cut(s) 2333
BspACI CCGC 6 cut(s) 93, 382, 1220, 1503, 1630, 1920
BspANI GGCC 7 cut(s) 96, 137, 381, 621, 1190, 1317, 2367
BspCNI CTCAG 9 cut(s) 457, 745, 1135, 1193, 1617, 1686, 1957, 2046, 2134
BspHI TCATGA 1 cut(s) 601
BspLI GGNNCC 4 cut(s) 396, 721, 1090, 2496
BspMAI CTGCAG 1 cut(s) 1856
BspPI GGATC 5 cut(s) 296, 1083, 1096, 1662, 1731
BspT107I GGYRCC 1 cut(s) 394
BspTI CTTAAG 2 cut(s) 1748, 1771
BspTNI GGTCTC 1 cut(s) 2313
BsrDI GCAATG 2 cut(s) 582, 1241
BsrFI RCCGGY 1 cut(s) 722
BsrI ACTGG 6 cut(s) 97, 278, 458, 1304, 2270, 2408
BssAI RCCGGY 1 cut(s) 722
BssECI CCNNGG 4 cut(s) 132, 1164, 1915, 2498
BssSI CACGAG 1 cut(s) 1319
BssT1I CCWWGG 1 cut(s) 132
Bst2BI CACGAG 1 cut(s) 1319
Bst2UI CCWGG 6 cut(s) 68, 1224, 1916, 2277, 2353, 2499
Bst4CI ACNGT 4 cut(s) 932, 1614, 1702, 2089
Bst6I CTCTTC 4 cut(s) 1126, 1485, 1733, 1810
BstAFI CTTAAG 2 cut(s) 1748, 1771
BstBAI YACGTR 2 cut(s) 544, 1280
BstF5I GGATG 3 cut(s) 405, 687, 796
BstH2I RGCGCY 2 cut(s) 669, 1756
BstHHI GCGC 2 cut(s) 668, 1755
BstMAI GTCTC 6 cut(s) 164, 333, 718, 1268, 2313, 2378
BstMWI GCNNNNNNNGC 8 cut(s) 77, 143, 358, 1740, 2011, 2131, 2153, 2197
BstNI CCWGG 6 cut(s) 68, 1224, 1916, 2277, 2353, 2499
BstSCI CCNGG 7 cut(s) 66, 1008, 1222, 1914, 2275, 2351, 2497
BstSFI CTRYAG 2 cut(s) 1083, 1852
BstSLI GKGCMC 4 cut(s) 397, 1010, 1612, 1803
BstV1I GCAGC 6 cut(s) 200, 848, 870, 880, 1838, 1866
BstV2I GAAGAC 4 cut(s) 119, 377, 944, 1272
BstX2I RGATCY 2 cut(s) 1088, 1723
BstYI RGATCY 2 cut(s) 1088, 1723
Bsu36I CCTNAGG 1 cut(s) 1944
BsuRI GGCC 7 cut(s) 96, 137, 381, 621, 1190, 1317, 2367
BtgZI GCGATG 1 cut(s) 501
BtsCI GGATG 3 cut(s) 405, 687, 796
BtsI GCAGTG 1 cut(s) 2344
BtsIMutI CAGTG 6 cut(s) 237, 1130, 1297, 1610, 1698, 2344
CciI TCATGA 1 cut(s) 601
CfoI GCGC 2 cut(s) 668, 1755
Cfr10I RCCGGY 1 cut(s) 722
Cfr13I GGNCC 4 cut(s) 25, 379, 825, 1912
Csp6I GTAC 7 cut(s) 233, 533, 758, 1460, 2082, 2380, 2432
CviAII CATG 4 cut(s) 602, 787, 1151, 2443
CviQI GTAC 7 cut(s) 233, 533, 758, 1460, 2082, 2380, 2432
EaeI YGGCCR 1 cut(s) 94
Eam1104I CTCTTC 4 cut(s) 1126, 1485, 1733, 1810
EarI CTCTTC 4 cut(s) 1126, 1485, 1733, 1810
Ecl136II GAGCTC 1 cut(s) 1978
Eco130I CCWWGG 1 cut(s) 132
Eco147I AGGCCT 3 cut(s) 137, 1317, 2367
Eco24I GRGCYC 2 cut(s) 1337, 1980
Eco31I GGTCTC 1 cut(s) 2313
Eco47I GGWCC 3 cut(s) 25, 825, 1912
Eco47III AGCGCT 2 cut(s) 667, 1754
Eco53kI GAGCTC 1 cut(s) 1978
Eco57I CTGAAG 4 cut(s) 73, 1250, 1284, 1714
Eco72I CACGTG 1 cut(s) 1280
Eco81I CCTNAGG 1 cut(s) 1944
EcoICRI GAGCTC 1 cut(s) 1978
EcoRI GAATTC 2 cut(s) 747, 1651
EcoRII CCWGG 6 cut(s) 66, 1222, 1914, 2275, 2351, 2497
EcoT14I CCWWGG 1 cut(s) 132
EcoT22I ATGCAT 1 cut(s) 424
EcoT38I GRGCYC 2 cut(s) 1337, 1980
ErhI CCWWGG 1 cut(s) 132
FaeI CATG 4 cut(s) 605, 790, 1154, 2446
FalI AAGNNNNNCTT 4 cut(s) 1223, 1255, 1948, 1980
FatI CATG 4 cut(s) 601, 786, 1150, 2442
FauI CCCGC 2 cut(s) 1227, 1913
FbaI TGATCA 2 cut(s) 1285, 2046
FblI GTMKAC 3 cut(s) 1434, 1522, 2400
FokI GGATG 3 cut(s) 392, 674, 803
FriOI GRGCYC 2 cut(s) 1337, 1980
GlaI GCGC 2 cut(s) 667, 1754
GsaI CCCAGC 1 cut(s) 1738
GsuI CTGGAG 3 cut(s) 738, 1043, 1160
HaeII RGCGCY 2 cut(s) 669, 1756
HaeIII GGCC 7 cut(s) 96, 137, 381, 621, 1190, 1317, 2367
HapII CCGG 5 cut(s) 285, 398, 723, 1010, 2023
HhaI GCGC 2 cut(s) 668, 1755
Hin1II CATG 4 cut(s) 605, 790, 1154, 2446
Hin6I GCGC 2 cut(s) 666, 1753
HinP1I GCGC 2 cut(s) 666, 1753
HpaII CCGG 5 cut(s) 285, 398, 723, 1010, 2023
HphI GGTGA 2 cut(s) 121, 530
Hpy166II GTNNAC 7 cut(s) 51, 825, 1061, 1435, 1523, 1705, 2401
Hpy188I TCNGA 7 cut(s) 321, 735, 780, 936, 1230, 1676, 1767
Hpy8I GTNNAC 7 cut(s) 51, 825, 1061, 1435, 1523, 1705, 2401
Hpy99I CGWCG 1 cut(s) 1514
HpyCH4III ACNGT 4 cut(s) 932, 1614, 1702, 2089
HpyCH4IV ACGT 3 cut(s) 543, 1279, 1591
HpyF10VI GCNNNNNNNGC 8 cut(s) 77, 143, 358, 1740, 2011, 2131, 2153, 2197
HpySE526I ACGT 3 cut(s) 543, 1279, 1591
Hsp92II CATG 4 cut(s) 605, 790, 1154, 2446
HspAI GCGC 2 cut(s) 666, 1753
Ksp22I TGATCA 2 cut(s) 1285, 2046
LmnI GCTCC 6 cut(s) 40, 287, 684, 719, 842, 1748
Lsp1109I GCAGC 6 cut(s) 200, 848, 870, 880, 1838, 1866
LweI GCATC 9 cut(s) 325, 409, 414, 696, 873, 1109, 1769, 2143, 2316
MaeII ACGT 3 cut(s) 543, 1279, 1591
MaeIII GTNAC 4 cut(s) 444, 727, 871, 2459
MfeI CAATTG 2 cut(s) 107, 897
MflI RGATCY 2 cut(s) 1088, 1723
MhlI GDGCHC 6 cut(s) 397, 1010, 1337, 1612, 1803, 1980
MlyI GAGTC 5 cut(s) 166, 934, 1106, 1440, 2008
MmeI TCCRAC 5 cut(s) 459, 485, 488, 1662, 1855
Mph1103I ATGCAT 1 cut(s) 424
MroXI GAANNNNTTC 1 cut(s) 1960
MspA1I CMGCKG 3 cut(s) 93, 861, 1734
MspCI CTTAAG 2 cut(s) 1748, 1771
MspI CCGG 5 cut(s) 285, 398, 723, 1010, 2023
MspR9I CCNGG 7 cut(s) 68, 1010, 1224, 1916, 2277, 2353, 2499
MunI CAATTG 2 cut(s) 107, 897
MvaI CCWGG 6 cut(s) 68, 1224, 1916, 2277, 2353, 2499
MwoI GCNNNNNNNGC 8 cut(s) 77, 143, 358, 1740, 2011, 2131, 2153, 2197
NciI CCSGG 1 cut(s) 1010
NlaIII CATG 4 cut(s) 605, 790, 1154, 2446
NlaIV GGNNCC 4 cut(s) 396, 721, 1090, 2496
NmuCI GTSAC 1 cut(s) 444
NsiI ATGCAT 1 cut(s) 424
PagI TCATGA 1 cut(s) 601
PceI AGGCCT 3 cut(s) 137, 1317, 2367
PcsI WCGNNNNNNNCGW 1 cut(s) 928
PdmI GAANNNNTTC 1 cut(s) 1960
PfeI GAWTC 5 cut(s) 457, 802, 924, 1986, 2356
PflFI GACNNNGTC 1 cut(s) 2324
PfoI TCCNGGA 1 cut(s) 2275
PleI GAGTC 5 cut(s) 165, 934, 1106, 1439, 2007
PmaCI CACGTG 1 cut(s) 1280
PmlI CACGTG 1 cut(s) 1280
PpsI GAGTC 5 cut(s) 165, 934, 1106, 1439, 2007
Ppu21I YACGTR 2 cut(s) 544, 1280
Psp124BI GAGCTC 1 cut(s) 1980
Psp6I CCWGG 6 cut(s) 66, 1222, 1914, 2275, 2351, 2497
PspCI CACGTG 1 cut(s) 1280
PspFI CCCAGC 1 cut(s) 1734
PspGI CCWGG 6 cut(s) 66, 1222, 1914, 2275, 2351, 2497
PspN4I GGNNCC 4 cut(s) 396, 721, 1090, 2496
PspPI GGNCC 4 cut(s) 25, 379, 825, 1912
PstI CTGCAG 1 cut(s) 1856
PsuI RGATCY 2 cut(s) 1088, 1723
PsyI GACNNNGTC 1 cut(s) 2324
PvuII CAGCTG 2 cut(s) 861, 1734
RsaI GTAC 7 cut(s) 234, 534, 759, 1461, 2083, 2381, 2433
RsaNI GTAC 7 cut(s) 233, 533, 758, 1460, 2082, 2380, 2432
SacI GAGCTC 1 cut(s) 1980
Sau96I GGNCC 4 cut(s) 25, 379, 825, 1912
ScaI AGTACT 1 cut(s) 759
SchI GAGTC 5 cut(s) 166, 934, 1106, 1440, 2008
ScrFI CCNGG 7 cut(s) 68, 1010, 1224, 1916, 2277, 2353, 2499
SduI GDGCHC 6 cut(s) 397, 1010, 1337, 1612, 1803, 1980
SfaNI GCATC 9 cut(s) 325, 409, 414, 696, 873, 1109, 1769, 2143, 2316
SfcI CTRYAG 2 cut(s) 1083, 1852
SinI GGWCC 3 cut(s) 25, 825, 1912
SmlI CTYRAG 5 cut(s) 1336, 1748, 1771, 2030, 2375
SmoI CTYRAG 5 cut(s) 1336, 1748, 1771, 2030, 2375
SseBI AGGCCT 3 cut(s) 137, 1317, 2367
SsiI CCGC 6 cut(s) 93, 382, 1220, 1503, 1630, 1920
SspI AATATT 1 cut(s) 1937
SstI GAGCTC 1 cut(s) 1980
StuI AGGCCT 3 cut(s) 137, 1317, 2367
StyD4I CCNGG 7 cut(s) 66, 1008, 1222, 1914, 2275, 2351, 2497
StyI CCWWGG 1 cut(s) 132
TaaI ACNGT 4 cut(s) 932, 1614, 1702, 2089
TaiI ACGT 3 cut(s) 546, 1282, 1594
TaqI TCGA 3 cut(s) 1110, 1509, 2310
TatI WGTACW 3 cut(s) 232, 757, 2431
TauI GCSGC 4 cut(s) 96, 384, 1506, 1632
TfiI GAWTC 5 cut(s) 457, 802, 924, 1986, 2356
TscAI CASTG 6 cut(s) 244, 1130, 1304, 1617, 1705, 2344
TseFI GTSAC 1 cut(s) 444
TseI GCWGC 6 cut(s) 188, 858, 861, 893, 1851, 1854
Tsp45I GTSAC 1 cut(s) 444
TspGWI ACGGA 5 cut(s) 53, 167, 294, 829, 2486
TspRI CASTG 6 cut(s) 244, 1130, 1304, 1617, 1705, 2344
Tth111I GACNNNGTC 1 cut(s) 2324
Vha464I CTTAAG 2 cut(s) 1748, 1771
VpaK11BI GGWCC 3 cut(s) 25, 825, 1912
XapI RAATTY 8 cut(s) 74, 346, 747, 812, 977, 1496, 1634, 1651
XbaI TCTAGA 1 cut(s) 607
XcmI CCANNNNNNNNNTGG 1 cut(s) 1304
XmiI GTMKAC 3 cut(s) 1434, 1522, 2400
XmnI GAANNNNTTC 1 cut(s) 1960
ZrmI AGTACT 1 cut(s) 759
Zsp2I ATGCAT 1 cut(s) 424
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.