Rroxscaffold_1G00075160

NB-ARC domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000540
Physical Location & Seq
Reverse (-)
95939332 .. 95941839
2508 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00075160.1

Sequence Viewer

Length: 2379 bp
ATGGTTCAGACTTTGACGGAGCAAGTGTTCACAGCTCTAATGAACCAGGCTGAATTTGCACTCGACTTCAGCGGCCAGTTTGAGCAGTTGAAGACGGGGCTTGATCTCACCAAGGCCTTACTGTCAGACACGGAGAGTCTCAACCACAAGAACAACACTATCAAGACAGCATTATTTGATCTAAGGGAAGTCATCTACAAAGCCGACGATGTACTCACCGATTGCTTGGTTAGAGATGAATACAGAAAGGAGGGATCCTGTTCCGGCTCCTTGCTTCACGATCCATTCTTCCTGCATCGTACAGGAAAGAAATTGAAAGACATCAATTTGCGTATGAAGGAGATAGAGAAGACCTTGGGCAGGTTTTTGAGGGCACCGGACAGCATCCATAGAGAAGATGCATACCAAGTTAGGGTGATGGTGTCACAGGACTGGAATCCAACTGAGATAATTGGGTTGGATAGCGATGTGGAGAAGATTAAGGGTTGGATTTTTGACACCAGCAATGTACTTCACCATGTAGGCATTGTGGGAATGGGTGGCTTGGGCAAAACCACCATTGCTCAAAAGATATTTCATGATCTAGAAGTAGTAGGCCACTTTCACAAGCTGATATGGGTGTGTGTTTCGCAATCATTCAGCGCTGAGCGGATTATGAGGAGCATCCTAGAAGGTCTAGGAGAAAACGTCTCAGGAGCCGGTGTTACTCAGATTTTGAGCAGAATTCAGCAAGTACTTAAAGATAAGAGCTGTCTGATTGTCATGGATGATGTGTGGAATCACACAGATGTTGATTGGTGGACCAACCTCTGTTCTGTTATACCCAAACAAAGCAGCTGCATCATAGTTACAACTAGAAATGAAGAAGCTGCAATTGGTATGGGAGTTGAAAGCTCACGAATCCATCGTCCGAAGACTCTTAATGATGAGGAAAGCTGGTGTTTATTCTCCAAGTTTGCATTTCCTACAAGCAAGGGATTGTGCCCGGAGGACAGGCTTGAGAAATTAGGAAAAGAACTCTTGAAGAAGTGTGGTGGACTTCCACTCGCAATCAAGACTATAGGATCCTTGTTGGCATCAAAGATCGACTACCCTTCACAGTGGACACGAGTTCTGGAGAGCTTTCATTCTTTGACAACCGAGGGAAAAACTAGCTCACTAAGCTCTGTTATGGCCTGTTTGCGATTGAGCTATGATGAACTTCCGCCTGGTTTGAAGCAATGCCTGCTGTGTTTCTCCATCTATCCTGAAGACTACGAGATACATGCTGATCAGTTAATCTACTGGTGGATCGGAGAAGGCCTCGTGCAAAGCAAGGGCTCAAGAACAGCAGTAGAAGTTGGATATGAGTATCTCACAGTACTAATCAGCAGATGCTTGGTTGAAATTGTGCAGAGGAGAGGATTTGATGGGAGAGTCTACAAGTGCAAAATACATGATATGGTGCGGGAACTGATAATATTGATTGCAGAAGAGGAGGAATTTTGCGGCTTCGACGAACGAGGCAGACAAGAATTGACAGCAAAGACTAGGTGGTTATGCTTTATTGATGAAATGGATGAAAAGTCCTTGAAACGTAGTTCAAAGCTTAGGGCATTGTTGCTGATGTCAAGCCGCGGATTTGACTTTGAAAGGAATTCGGGGTTTTTGCTGCGATCCCTCAGAATGTTGGATTTATCAAACTGCACTGTGGATGAGCATTGTGTGGAGGATCTCTTCAGCTGGATCAGCTCCCTTAAGCGCTTAGCATCTCTGAACTTAAGTGGTATTCAAGCCCTGAAAGAAGTGCCCTCTTCAATTCATAAACTCCTCAACCTCCAACTTTTGCTTTTAACAGGTTGCAGCAATCTAGTAAAAATACACCCTTCCATCACCAATTTGAAGAAGTTGATTATTTTGGACCTAGGCGGGTGTCCAATCCAATACTTGCCTCAGGGATTGGGAAGGCTCTCTTATCTTCAAGAGCTCTCCGGATTCAAGGTCCTTAGAAGGCTCAATGTTCTAGCTATTGATGCAGAAGATTGTAGAAATAACAATGTTTTGGAAATGCTAGAAGGGCTGACCCCTCCTCCAAGCCTCCAAGAGCTATATCTTAGGAATTACAAGAAGGAAACTTTGCCCAACTGGGTTAATCCTGGACAACTTTCACGATTGCAGTATCTTTGCATCGAGAATGGAGACCTTGTCAAGCTAGGCAGTGGTCAAACCACCTGGAATCTTGAAGGCCTATGTCTCAAGTACTTGATGAGGCTAGAGGTAGACTGGAAGGACTTGGAGAAGGATATGCCTGTACTTCACTACATGGAGATCAGTCATTGTTACAACTTGACGGATTTCCCTTGTTCGGTTATGGAACCTGGGGTCTGGAGAAAGCCTTAA

Protein Analysis

792

Amino Acids

90.09

Weight (kDa)

6.08

Isoelectric Point (pI)

40.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 1 - 87 7.2e-13 Rx N-terminal domain
NB-ARC PF00931 155 - 324 2.8e-40 NB-ARC domain
AAA_22 PF13401 176 - 258 4.2e-06 AAA domain
WHD_DRP PF23559 414 - 486 6e-19 Disease resistance protein Winged helix domain
LRR_14 PF23598 552 - 661 5.6e-11 Leucine-rich repeat region
LRR_14 PF23598 662 - 724 6.5e-06 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 673 - 729 1.2e-07 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0000540)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G50950 AT3G50950
fragaria_vesca FvH4_1g12710 FvH4_1g12710 FvH4_3g00820 FvH4_3g00820 FvH4_3g00820 FvH4_3g00820
malus_domestica MD02G1141100.v1.1 MD10G1333100.v1.1
prunus_persica Prupe.4G009300_v2.0.a1 Prupe.4G009300_v2.0.a1 Prupe.4G009300_v2.0.a1 Prupe.6G150700_v2.0.a1 Prupe.7G160100_v2.0.a1 Prupe.7G160100_v2.0.a1
pyrus_communis pycom02g11110 pycom10g28240 pycom10g28340 pycom10g28360 pycom10g28450
rosa_chinensis RchiOBHm_Chr2g0100711 RchiOBHm_Chr3g0485861 RchiOBHm_Chr4g0391451 RchiOBHm_Chr4g0391591 RchiOBHm_Chr4g0391601 RchiOBHm_Chr4g0391681 RchiOBHm_Chr5g0001141 RchiOBHm_Chr5g0001221
rosa_laevigata RLG00000017001
rosa_multiflora Rmu_co8260007.1_g000001 Rmu_co8260103.1_g000001 Rmu_co8519155.1_g000001 Rmu_sc0000060.1_g000050 Rmu_sc0000255.1_g000036 Rmu_sc0001174.1_g000023 Rmu_sc0001174.1_g000025 Rmu_sc0001659.1_g000009 Rmu_sc0001706.1_g000009 Rmu_sc0001706.1_g000024 Rmu_sc0001706.1_g000035 Rmu_sc0004189.1_g000018 Rmu_sc0004189.1_g000030 Rmu_sc0004862.1_g000002 Rmu_sc0004876.1_g000038 Rmu_sc0023741.1_g000004
rosa_roxburghii Rroxscaffold_1G00075100 Rroxscaffold_1G00075160 Rroxscaffold_1G00075210 Rroxscaffold_5G00337160 Rroxscaffold_5G00386590 Rroxscaffold_6G00396300
rosa_rugosa Rorug03G0336200 Rorug03G0336300 Rorug04G0389400
rosa_samantha Rh2AG140400 Rh2BG144100 Rh2CG145400 Rh2DG144900 Rh3AG271200 Rh4AG038000 Rh4AG038700 Rh4BG032000 Rh4BG032800 Rh4CG041100 Rh4CG041300 Rh4DG035500 Rh4DG035600 Rh5AG008800 Rh5AG009100 Rh5BG010900 Rh5BG011500 Rh5BG011600 Rh5BG012000 Rh5CG009400 Rh5CG010000 Rh5DG009800
rosa_wichuraiana Rw2G010940 Rw4G002950 Rw4G002990 Rw4G003000 Rw5G000880 Rw5G000950 Rw5G000960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 351
AccB1I GGYRCC 1 cut(s) 373
AccBSI CCGCTC 1 cut(s) 649
AccI GTMKAC 2 cut(s) 1419, 2259
AccII CGCG 1 cut(s) 1617
AccIII TCCGGA 1 cut(s) 1970
AciI CCGC 8 cut(s) 72, 649, 1205, 1447, 1488, 1615, 1617, 1908
AclWI GGATC 9 cut(s) 249, 262, 275, 1059, 1072, 1298, 1650, 1719, 1733
AcoI YGGCCR 1 cut(s) 73
AcsI RAATTY 4 cut(s) 53, 723, 1481, 1636
AcuI CTGAAG 3 cut(s) 52, 1269, 1702
AfaI GTAC 7 cut(s) 213, 301, 510, 735, 1362, 2240, 2292
AfeI AGCGCT 2 cut(s) 643, 1742
AfiI CCNNNNNNNGG 3 cut(s) 360, 412, 2344
AflII CTTAAG 2 cut(s) 1736, 1759
AjnI CCWGG 5 cut(s) 45, 1207, 2134, 2210, 2356
AjuI GAANNNNNNNTTGG 2 cut(s) 858, 890
AloI GAACNNNNNNTCC 2 cut(s) 11, 43
Alw21I GWGCWC 1 cut(s) 1968
Alw26I GTCTC 4 cut(s) 143, 694, 2172, 2237
AlwI GGATC 9 cut(s) 249, 262, 275, 1059, 1072, 1298, 1650, 1719, 1733
Aor13HI TCCGGA 1 cut(s) 1970
Aor51HI AGCGCT 2 cut(s) 643, 1742
AoxI GGCC 6 cut(s) 73, 114, 595, 1173, 1300, 2224
ApeKI GCWGC 5 cut(s) 834, 837, 869, 1651, 1842
ApoI RAATTY 4 cut(s) 53, 723, 1481, 1636
ArsI GACNNNNNNTTYG 2 cut(s) 892, 924
AspA2I CCTAGG 1 cut(s) 1903
AspLEI GCGC 2 cut(s) 644, 1743
AspS9I GGNCC 3 cut(s) 801, 1900, 1981
AsuC2I CCSGG 1 cut(s) 986
AsuHPI GGTGA 5 cut(s) 100, 208, 427, 506, 1864
AvaII GGWCC 3 cut(s) 801, 1900, 1981
AvrII CCTAGG 1 cut(s) 1903
AxyI CCTNAGG 1 cut(s) 1932
BaeGI GKGCMC 3 cut(s) 376, 986, 1791
BamHI GGATCC 2 cut(s) 254, 1064
BanI GGYRCC 1 cut(s) 373
BanII GRGCYC 2 cut(s) 1322, 1968
BauI CACGAG 2 cut(s) 1107, 1304
BbsI GAAGAC 4 cut(s) 98, 356, 920, 1257
Bbv12I GWGCWC 1 cut(s) 1968
BbvI GCAGC 5 cut(s) 824, 846, 856, 1638, 1854
BccI CCATC 5 cut(s) 412, 912, 1247, 1403, 1877
BcgI CGANNNNNNTGC 2 cut(s) 1247, 1281
BciT130I CCWGG 5 cut(s) 47, 1209, 2136, 2212, 2358
BclI TGATCA 1 cut(s) 1270
BcnI CCSGG 1 cut(s) 986
BcoDI GTCTC 4 cut(s) 143, 694, 2172, 2237
BfmI CTRYAG 1 cut(s) 1059
BfoI RGCGCY 2 cut(s) 645, 1744
BfrI CTTAAG 2 cut(s) 1736, 1759
BfuAI ACCTGC 1 cut(s) 351
BisI GCNGC 8 cut(s) 73, 835, 838, 870, 1489, 1615, 1652, 1843
BlnI CCTAGG 1 cut(s) 1903
BlpI GCTNAGC 2 cut(s) 645, 1744
BlsI GCNGC 8 cut(s) 74, 836, 839, 871, 1490, 1616, 1653, 1844
BmcAI AGTACT 3 cut(s) 735, 1362, 2240
Bme1390I CCNGG 6 cut(s) 47, 986, 1209, 2136, 2212, 2358
Bme18I GGWCC 3 cut(s) 801, 1900, 1981
BmgT120I GGNCC 3 cut(s) 801, 1900, 1981
BmiI GGNNCC 6 cut(s) 256, 268, 375, 697, 1066, 2355
BmrFI CCNGG 6 cut(s) 47, 986, 1209, 2136, 2212, 2358
BmrI ACTGGG 1 cut(s) 2134
BmuI ACTGGG 1 cut(s) 2134
BpiI GAAGAC 4 cut(s) 98, 356, 920, 1257
BplI GAGNNNNNCTC 2 cut(s) 1287, 1319
BpmI CTGGAG 1 cut(s) 1136
Bpu10I CCTNAGC 1 cut(s) 1589
Bpu1102I GCTNAGC 2 cut(s) 645, 1744
BpuEI CTTGAG 3 cut(s) 1019, 1306, 2219
BpuMI CCSGG 1 cut(s) 986
BsaI GGTCTC 1 cut(s) 2172
BsaJI CCNNGG 6 cut(s) 111, 354, 1140, 1615, 1903, 2357
BsaWI WCCGGW 2 cut(s) 376, 1970
Bsc4I CCNNNNNNNGG 3 cut(s) 360, 412, 2344
Bse118I RCCGGY 1 cut(s) 698
Bse1I ACTGG 5 cut(s) 76, 437, 1289, 2129, 2267
Bse21I CCTNAGG 1 cut(s) 1932
Bse3DI GCAATG 3 cut(s) 511, 558, 1226
BseAI TCCGGA 1 cut(s) 1970
BseBI CCWGG 5 cut(s) 47, 1209, 2136, 2212, 2358
BseDI CCNNGG 6 cut(s) 111, 354, 1140, 1615, 1903, 2357
BseGI GGATG 5 cut(s) 384, 663, 772, 1564, 1699
BseLI CCNNNNNNNGG 3 cut(s) 360, 412, 2344
BseMI GCAATG 3 cut(s) 511, 558, 1226
BseMII CTCAG 6 cut(s) 435, 636, 705, 722, 1675, 1946
BseNI ACTGG 5 cut(s) 76, 437, 1289, 2129, 2267
BseRI GAGGAG 5 cut(s) 673, 1411, 1490, 1799, 2058
BseSI GKGCMC 3 cut(s) 376, 986, 1791
BseXI GCAGC 5 cut(s) 824, 846, 856, 1638, 1854
BsgI GTGCAG 2 cut(s) 1412, 1669
Bsh1236I CGCG 1 cut(s) 1617
BshFI GGCC 6 cut(s) 75, 116, 597, 1175, 1302, 2226
BshNI GGYRCC 1 cut(s) 373
BsiHKAI GWGCWC 1 cut(s) 1968
BsiSI CCGG 5 cut(s) 264, 377, 699, 986, 1971
BslI CCNNNNNNNGG 3 cut(s) 360, 412, 2344
BsmAI GTCTC 4 cut(s) 143, 694, 2172, 2237
BsmBI CGTCTC 1 cut(s) 694
BsnI GGCC 6 cut(s) 75, 116, 597, 1175, 1302, 2226
Bso31I GGTCTC 1 cut(s) 2172
Bsp1286I GDGCHC 5 cut(s) 376, 986, 1322, 1791, 1968
Bsp13I TCCGGA 1 cut(s) 1970
Bsp1720I GCTNAGC 2 cut(s) 645, 1744
BspACI CCGC 8 cut(s) 72, 649, 1205, 1447, 1488, 1615, 1617, 1908
BspANI GGCC 6 cut(s) 75, 116, 597, 1175, 1302, 2226
BspCNI CTCAG 6 cut(s) 436, 637, 704, 721, 1674, 1945
BspEI TCCGGA 1 cut(s) 1970
BspFNI CGCG 1 cut(s) 1617
BspHI TCATGA 1 cut(s) 577
BspLI GGNNCC 6 cut(s) 256, 268, 375, 697, 1066, 2355
BspMI ACCTGC 1 cut(s) 351
BspPI GGATC 9 cut(s) 249, 262, 275, 1059, 1072, 1298, 1650, 1719, 1733
BspT107I GGYRCC 1 cut(s) 373
BspTI CTTAAG 2 cut(s) 1736, 1759
BspTNI GGTCTC 1 cut(s) 2172
BsrBI CCGCTC 1 cut(s) 649
BsrDI GCAATG 3 cut(s) 511, 558, 1226
BsrFI RCCGGY 1 cut(s) 698
BsrI ACTGG 5 cut(s) 76, 437, 1289, 2129, 2267
BssAI RCCGGY 1 cut(s) 698
BssECI CCNNGG 6 cut(s) 111, 354, 1140, 1615, 1903, 2357
BssSI CACGAG 2 cut(s) 1107, 1304
BssT1I CCWWGG 3 cut(s) 111, 354, 1903
Bst2BI CACGAG 2 cut(s) 1107, 1304
Bst2UI CCWGG 5 cut(s) 47, 1209, 2136, 2212, 2358
Bst4CI ACNGT 4 cut(s) 123, 1101, 1360, 1690
Bst6I CTCTTC 3 cut(s) 1467, 1721, 1798
BstAFI CTTAAG 2 cut(s) 1736, 1759
BstAPI GCANNNNNTGC 1 cut(s) 1225
BstC8I GCNNGC 1 cut(s) 1226
BstDSI CCRYGG 1 cut(s) 1615
BstENI CCTNNNNNAGG 1 cut(s) 358
BstF5I GGATG 5 cut(s) 384, 663, 772, 1564, 1699
BstFNI CGCG 1 cut(s) 1617
BstH2I RGCGCY 2 cut(s) 645, 1744
BstHHI GCGC 2 cut(s) 644, 1743
BstMAI GTCTC 4 cut(s) 143, 694, 2172, 2237
BstMWI GCNNNNNNNGC 6 cut(s) 56, 1161, 1225, 1728, 2012, 2056
BstNI CCWGG 5 cut(s) 47, 1209, 2136, 2212, 2358
BstNSI RCATGY 1 cut(s) 1268
BstSCI CCNGG 6 cut(s) 45, 984, 1207, 2134, 2210, 2356
BstSFI CTRYAG 1 cut(s) 1059
BstSLI GKGCMC 3 cut(s) 376, 986, 1791
BstUI CGCG 1 cut(s) 1617
BstV1I GCAGC 5 cut(s) 824, 846, 856, 1638, 1854
BstV2I GAAGAC 4 cut(s) 98, 356, 920, 1257
BstX2I RGATCY 3 cut(s) 254, 1064, 1711
BstYI RGATCY 3 cut(s) 254, 1064, 1711
Bsu36I CCTNAGG 1 cut(s) 1932
BsuRI GGCC 6 cut(s) 75, 116, 597, 1175, 1302, 2226
BtgI CCRYGG 1 cut(s) 1615
BtgZI GCGATG 1 cut(s) 480
BtsCI GGATG 5 cut(s) 384, 663, 772, 1564, 1699
BtsI GCAGTG 1 cut(s) 2203
BtsIMutI CAGTG 3 cut(s) 1106, 1686, 2203
BveI ACCTGC 1 cut(s) 351
Cac8I GCNNGC 1 cut(s) 1226
CciI TCATGA 1 cut(s) 577
CfoI GCGC 2 cut(s) 644, 1743
Cfr10I RCCGGY 1 cut(s) 698
Cfr13I GGNCC 3 cut(s) 801, 1900, 1981
Cfr42I CCGCGG 1 cut(s) 1618
Csp6I GTAC 7 cut(s) 212, 300, 509, 734, 1361, 2239, 2291
CviAII CATG 6 cut(s) 518, 578, 763, 1265, 1436, 2302
CviQI GTAC 7 cut(s) 212, 300, 509, 734, 1361, 2239, 2291
EaeI YGGCCR 1 cut(s) 73
Eam1104I CTCTTC 3 cut(s) 1467, 1721, 1798
EarI CTCTTC 3 cut(s) 1467, 1721, 1798
EciI GGCGGA 1 cut(s) 1194
Ecl136II GAGCTC 1 cut(s) 1966
Eco130I CCWWGG 3 cut(s) 111, 354, 1903
Eco147I AGGCCT 3 cut(s) 116, 1302, 2226
Eco24I GRGCYC 2 cut(s) 1322, 1968
Eco31I GGTCTC 1 cut(s) 2172
Eco47I GGWCC 3 cut(s) 801, 1900, 1981
Eco47III AGCGCT 2 cut(s) 643, 1742
Eco53kI GAGCTC 1 cut(s) 1966
Eco57I CTGAAG 3 cut(s) 52, 1269, 1702
Eco81I CCTNAGG 1 cut(s) 1932
EcoICRI GAGCTC 1 cut(s) 1966
EcoNI CCTNNNNNAGG 1 cut(s) 358
EcoO109I RGGNCCY 1 cut(s) 1981
EcoRI GAATTC 2 cut(s) 723, 1636
EcoRII CCWGG 5 cut(s) 45, 1207, 2134, 2210, 2356
EcoT14I CCWWGG 3 cut(s) 111, 354, 1903
EcoT22I ATGCAT 1 cut(s) 403
EcoT38I GRGCYC 2 cut(s) 1322, 1968
ErhI CCWWGG 3 cut(s) 111, 354, 1903
Esp3I CGTCTC 1 cut(s) 694
FaeI CATG 6 cut(s) 521, 581, 766, 1268, 1439, 2305
FalI AAGNNNNNCTT 2 cut(s) 1936, 1968
FatI CATG 6 cut(s) 517, 577, 762, 1264, 1435, 2301
FauI CCCGC 2 cut(s) 1440, 1901
FbaI TGATCA 1 cut(s) 1270
FblI GTMKAC 2 cut(s) 1419, 2259
Fnu4HI GCNGC 8 cut(s) 73, 835, 838, 870, 1489, 1615, 1652, 1843
FokI GGATG 5 cut(s) 371, 650, 779, 1571, 1706
FriOI GRGCYC 2 cut(s) 1322, 1968
Fsp4HI GCNGC 8 cut(s) 73, 835, 838, 870, 1489, 1615, 1652, 1843
GlaI GCGC 2 cut(s) 643, 1742
GluI GCNGC 8 cut(s) 73, 835, 838, 870, 1489, 1615, 1652, 1843
GsuI CTGGAG 1 cut(s) 1136
HaeII RGCGCY 2 cut(s) 645, 1744
HaeIII GGCC 6 cut(s) 75, 116, 597, 1175, 1302, 2226
HapII CCGG 5 cut(s) 264, 377, 699, 986, 1971
HhaI GCGC 2 cut(s) 644, 1743
Hin1II CATG 6 cut(s) 521, 581, 766, 1268, 1439, 2305
Hin6I GCGC 2 cut(s) 642, 1741
HinP1I GCGC 2 cut(s) 642, 1741
HindIII AAGCTT 1 cut(s) 1586
HinfI GANTC 8 cut(s) 136, 436, 778, 900, 916, 1416, 1974, 2215
HpaII CCGG 5 cut(s) 264, 377, 699, 986, 1971
HphI GGTGA 5 cut(s) 100, 208, 427, 506, 1864
Hpy166II GTNNAC 6 cut(s) 30, 801, 1037, 1104, 1420, 2260
Hpy188I TCNGA 8 cut(s) 9, 127, 711, 756, 912, 1295, 1664, 1755
Hpy8I GTNNAC 6 cut(s) 30, 801, 1037, 1104, 1420, 2260
Hpy99I CGWCG 2 cut(s) 209, 1499
HpyCH4III ACNGT 4 cut(s) 123, 1101, 1360, 1690
HpyCH4IV ACGT 2 cut(s) 687, 1576
HpyF10VI GCNNNNNNNGC 6 cut(s) 56, 1161, 1225, 1728, 2012, 2056
HpySE526I ACGT 2 cut(s) 687, 1576
Hsp92II CATG 6 cut(s) 521, 581, 766, 1268, 1439, 2305
HspAI GCGC 2 cut(s) 642, 1741
Kpn2I TCCGGA 1 cut(s) 1970
Ksp22I TGATCA 1 cut(s) 1270
KspI CCGCGG 1 cut(s) 1618
LmnI GCTCC 5 cut(s) 19, 272, 660, 695, 1736
Lsp1109I GCAGC 5 cut(s) 824, 846, 856, 1638, 1854
MaeII ACGT 2 cut(s) 687, 1576
MaeIII GTNAC 4 cut(s) 423, 703, 847, 2318
MbiI CCGCTC 1 cut(s) 649
MfeI CAATTG 1 cut(s) 873
MflI RGATCY 3 cut(s) 254, 1064, 1711
MhlI GDGCHC 5 cut(s) 376, 986, 1322, 1791, 1968
MlyI GAGTC 3 cut(s) 145, 910, 1425
MmeI TCCRAC 6 cut(s) 438, 464, 467, 1321, 1650, 1843
Mph1103I ATGCAT 1 cut(s) 403
MroI TCCGGA 1 cut(s) 1970
MseI TTAA 9 cut(s) 480, 738, 921, 1277, 1737, 1760, 1832, 2130, 2377
MslI CAYNNNNRTG 1 cut(s) 786
MspA1I CMGCKG 4 cut(s) 72, 837, 1617, 1722
MspCI CTTAAG 2 cut(s) 1736, 1759
MspI CCGG 5 cut(s) 264, 377, 699, 986, 1971
MspR9I CCNGG 6 cut(s) 47, 986, 1209, 2136, 2212, 2358
MunI CAATTG 1 cut(s) 873
MvaI CCWGG 5 cut(s) 47, 1209, 2136, 2212, 2358
MvnI CGCG 1 cut(s) 1617
MwoI GCNNNNNNNGC 6 cut(s) 56, 1161, 1225, 1728, 2012, 2056
NciI CCSGG 1 cut(s) 986
NlaIII CATG 6 cut(s) 521, 581, 766, 1268, 1439, 2305
NlaIV GGNNCC 6 cut(s) 256, 268, 375, 697, 1066, 2355
NmuCI GTSAC 1 cut(s) 423
NsiI ATGCAT 1 cut(s) 403
NspI RCATGY 1 cut(s) 1268
PagI TCATGA 1 cut(s) 577
PceI AGGCCT 3 cut(s) 116, 1302, 2226
PcsI WCGNNNNNNNCGW 1 cut(s) 904
PfeI GAWTC 5 cut(s) 436, 778, 900, 1974, 2215
PflFI GACNNNGTC 1 cut(s) 2183
PfoI TCCNGGA 1 cut(s) 2134
PkrI GCNGC 8 cut(s) 74, 836, 839, 871, 1490, 1616, 1653, 1844
PleI GAGTC 3 cut(s) 144, 910, 1424
PpsI GAGTC 3 cut(s) 144, 910, 1424
PpuMI RGGWCCY 1 cut(s) 1981
Psp124BI GAGCTC 1 cut(s) 1968
Psp5II RGGWCCY 1 cut(s) 1981
Psp6I CCWGG 5 cut(s) 45, 1207, 2134, 2210, 2356
PspGI CCWGG 5 cut(s) 45, 1207, 2134, 2210, 2356
PspN4I GGNNCC 6 cut(s) 256, 268, 375, 697, 1066, 2355
PspPI GGNCC 3 cut(s) 801, 1900, 1981
PspPPI RGGWCCY 1 cut(s) 1981
PsuI RGATCY 3 cut(s) 254, 1064, 1711
PsyI GACNNNGTC 1 cut(s) 2183
PvuII CAGCTG 2 cut(s) 837, 1722
RsaI GTAC 7 cut(s) 213, 301, 510, 735, 1362, 2240, 2292
RsaNI GTAC 7 cut(s) 212, 300, 509, 734, 1361, 2239, 2291
RseI CAYNNNNRTG 1 cut(s) 786
SacI GAGCTC 1 cut(s) 1968
SacII CCGCGG 1 cut(s) 1618
SaqAI TTAA 9 cut(s) 480, 738, 921, 1277, 1737, 1760, 1832, 2130, 2377
SatI GCNGC 8 cut(s) 73, 835, 838, 870, 1489, 1615, 1652, 1843
Sau96I GGNCC 3 cut(s) 801, 1900, 1981
ScaI AGTACT 3 cut(s) 735, 1362, 2240
SchI GAGTC 3 cut(s) 145, 910, 1425
ScrFI CCNGG 6 cut(s) 47, 986, 1209, 2136, 2212, 2358
SduI GDGCHC 5 cut(s) 376, 986, 1322, 1791, 1968
SfcI CTRYAG 1 cut(s) 1059
Sfr303I CCGCGG 1 cut(s) 1618
SgrBI CCGCGG 1 cut(s) 1618
SinI GGWCC 3 cut(s) 801, 1900, 1981
SmiMI CAYNNNNRTG 1 cut(s) 786
SmlI CTYRAG 5 cut(s) 998, 1321, 1736, 1759, 2234
SmoI CTYRAG 5 cut(s) 998, 1321, 1736, 1759, 2234
SseBI AGGCCT 3 cut(s) 116, 1302, 2226
SsiI CCGC 8 cut(s) 72, 649, 1205, 1447, 1488, 1615, 1617, 1908
SspI AATATT 1 cut(s) 1461
SstI GAGCTC 1 cut(s) 1968
StuI AGGCCT 3 cut(s) 116, 1302, 2226
StyD4I CCNGG 6 cut(s) 45, 984, 1207, 2134, 2210, 2356
StyI CCWWGG 3 cut(s) 111, 354, 1903
TaaI ACNGT 4 cut(s) 123, 1101, 1360, 1690
TaiI ACGT 2 cut(s) 690, 1579
TaqI TCGA 4 cut(s) 63, 1086, 1494, 2169
TatI WGTACW 6 cut(s) 211, 508, 733, 1360, 2238, 2290
TauI GCSGC 3 cut(s) 75, 1491, 1617
TfiI GAWTC 5 cut(s) 436, 778, 900, 1974, 2215
Tru1I TTAA 9 cut(s) 480, 738, 921, 1277, 1737, 1760, 1832, 2130, 2377
Tru9I TTAA 9 cut(s) 480, 738, 921, 1277, 1737, 1760, 1832, 2130, 2377
TscAI CASTG 3 cut(s) 1106, 1693, 2203
TseFI GTSAC 1 cut(s) 423
TseI GCWGC 5 cut(s) 834, 837, 869, 1651, 1842
Tsp45I GTSAC 1 cut(s) 423
TspGWI ACGGA 3 cut(s) 32, 146, 2345
TspRI CASTG 3 cut(s) 1106, 1693, 2203
Tth111I GACNNNGTC 1 cut(s) 2183
Vha464I CTTAAG 2 cut(s) 1736, 1759
VpaK11BI GGWCC 3 cut(s) 801, 1900, 1981
XagI CCTNNNNNAGG 1 cut(s) 358
XapI RAATTY 4 cut(s) 53, 723, 1481, 1636
XbaI TCTAGA 1 cut(s) 583
XceI RCATGY 1 cut(s) 1268
XmaJI CCTAGG 1 cut(s) 1903
XmiI GTMKAC 2 cut(s) 1419, 2259
ZrmI AGTACT 3 cut(s) 735, 1362, 2240
Zsp2I ATGCAT 1 cut(s) 403
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.