Rh5BG012000

NB-ARC domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0000540
Physical Location & Seq
Forward (+)
905669 .. 909589
3921 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG012000.1

Sequence Viewer

Length: 2517 bp
ATGTCGTTAATAGTGGAGGCTATGGTCCAGACTCTAACGGAGCAAGTGTTCACAGCTCTAATGAACCAGGCTGAGTTTGCACTCGACTTCAGCGGCCAGTTTGAGCAGTTGAAGACAGGGCTTGATCTCACCAGGGCCTTACTTGCAGACACGGAGAGTCTCAACCACAAGAACAACACTGTCAAGGCAGCATTGTTTGACTTAAGGGAAGTCATCTACAAAGCAGACGATGTACTCACTGATTGCTTGGTTAGAGATGAATACAGAAAGGATGGATCTTGTTCCGGTTCCTGGCTTCACGATCCATTCTTCCTGCATCGTACAGGAAAGAAATTGAGAGACATCAATTTGCGTATGAAGGAGATAGAGAAGACCTTGGGCCGGTTTTTGAGGGCACCAGACAGCATCCATAGAGAAGATGCATACCAAGTTAGAGTGATGGTGTCACAGGACTGGAATCCAACTGAGATAATTGGGTTGGATAGCGATGTGGAGAAGATTAAGGGTTGGATTTTCGACACCAGCAAGTTACTTCACCACGTAGGCATTGTGGGCATGGGAGGCCTTGGGAAAACCACCATTGCTCAAAAGATATTTCATGATCTACAAGTTGTAGGCCACTTTCACAAGCTGATATGGGTGTGTGTTTCGCAATCATTCAGCGCTGAGCGGATTATGAGGAGCATCCTAGAAGGTCTAGGAGAAAATGTCTCTGGAGCAAATGTTACTCAGATTTTGAGCAGAATTCAGCAAGTACTTAAAGATAAGAGCTGTTTGATTGTCATGGATGATGTGTGGAATCACACAGAATTTGATTGGTGGACCAATCTCTGCTCCGTTATACCCAAACAAAGCAGCTGCATCATAGTTACAACTAGAAATGAAGAAGCTGCAATTGGTATGGGAGTTGAAAGCTCACGAATCCACCGTCCCAAGACTCTTAATGATGAGGAAAGCTGGTGTTTATTCTCGAAGTTTGCATTTCCTACAAGCAAGGGATTGTGCCCGGAGGACAGGATTGAGAAATTAGGAAAAGAACTCTTGAAGAAGTGTGGTGGACTCCCGCTTGCAATTAAGACTATAGGATCCTTGTTGGCATCAAAGATCGACTCCCCTTCTCAGTGGAGGCGAGTTCTAGAGAGCTTTCATGCTTTGACAACCGAGGGAAAAACTAGCTCAGTTATCGCCTCTTTGCGATTGAGCTATGATGAACTCCCGCCTGGTCTGAAGCAATGCCTGCTGTGTTTCTCCATCTATCCTGAAGACTATGAGATACGTGCTGATCAGTTGATCCACTGGTGGATTGGAGAAGGCATCGTGCAAAGCAAGGGCTCAAGAACAGCAGTAGAAGTGGGATATGAGTATCTCGCAGAACTAATCAGCAGATGCTTGGTTGAAATTGTGCAGAGGAGAGGATTTGATGGGAGAGTCTACAAGTGCAAGATACACGATATGGTGCGGGAACTGATAATAATGATTGCAGAGGAAGAGGAGTTTTGCGGCTTTGACGAACGAGGCAGACAGGAATTGACAGCAAAGACTAGGTGGTTATGCTTTATTGATGAAATGGAGGAGAAGTCCTTGAAACGTAGTTCAAAGCTCAGGGCATTGTTGCTGATGTCAAGCCGCAGATTTGACTTTGAAAGGAATTCGGGGTTTCTGCGATCCCTCAGAATGTTGGATCTATCAAACTGCACTGTGGATGAGCATTGTGTGGAGGATCTCTTCAGCTGGATCAGCTCCCTTAAGCGCTTGGCATCTCTGAACTTAAGTGGTATTCAAGCCCTGAAAGAAGTGCCCTCTTCAATTCATAAACTCCTCAACCTCCAACTTTTGCTTTTAACAGGTTGCAGCAATCTAGTAAAAATACACCCTTCCATCACTAATTTGAAGAAGCTGATTATTTTGGACCTAGGCGGGTGTCCAATCCAATACTTGCCTCAGGGATTGGGAAGGCTTTCTTATCTTCAAGAGCTCTCTGGATTCAAGGTTGTGAGTCAGCCTAGAAAGCCAAGTTTCCGGCTTCTTGAGATTAGAGAATTGATCAACCTGAGAGTACTAAGAATACATGTAAGCAGTACCACAGTTATCGCAGACAATGAACTTGATGTCCTCTCTCAGCTTAGAAGGCTCAATGTTCTAGCTATTGATGCAGAAGATTGTAGAAATAACAATGTTTTGGAAATGCTAGAAGGGCTGACCCCTCCTCCAAGCCTCCAAGAGCTATATCTTAGGAATTACAAGAAGGAAACTTTGCCCAACTGGGTTAATCCTGGACAACTTTCGCGATTGCAGTATCTTTGCATCGAGAATGGAGACCTTGTCAAGCTAGGCAGTGGTCAAACCACCTGGAATCTTGAAGGCCTATGTCTCAAGTACTTGATGAGGCTAGAGGTAGACTGGAAGGACTTGGAGAAGGATATGCCTGTACTTCACTACATGGAGATCAGTCATTGTTACAACTTGACGGATTTCCCTTGTTCGGTTATGGAACCTGGGGTCTGGAGAAAGCATTAA

Protein Analysis

838

Amino Acids

95.67

Weight (kDa)

6.43

Isoelectric Point (pI)

44.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 8 - 93 5e-13 Rx N-terminal domain
NB-ARC PF00931 162 - 331 2.7e-40 NB-ARC domain
AAA_22 PF13401 183 - 265 1.2e-06 AAA domain
WHD_DRP PF23559 418 - 490 5.8e-20 Disease resistance protein Winged helix domain
LRR_14 PF23598 553 - 770 8.4e-28 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 690 - 774 1e-08 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000540)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G50950 AT3G50950
fragaria_vesca FvH4_1g12710 FvH4_1g12710 FvH4_3g00820 FvH4_3g00820 FvH4_3g00820 FvH4_3g00820
malus_domestica MD02G1141100.v1.1 MD10G1333100.v1.1
prunus_persica Prupe.4G009300_v2.0.a1 Prupe.4G009300_v2.0.a1 Prupe.4G009300_v2.0.a1 Prupe.6G150700_v2.0.a1 Prupe.7G160100_v2.0.a1 Prupe.7G160100_v2.0.a1
pyrus_communis pycom02g11110 pycom10g28240 pycom10g28340 pycom10g28360 pycom10g28450
rosa_chinensis RchiOBHm_Chr2g0100711 RchiOBHm_Chr3g0485861 RchiOBHm_Chr4g0391451 RchiOBHm_Chr4g0391591 RchiOBHm_Chr4g0391601 RchiOBHm_Chr4g0391681 RchiOBHm_Chr5g0001141 RchiOBHm_Chr5g0001221
rosa_laevigata RLG00000017001
rosa_multiflora Rmu_co8260007.1_g000001 Rmu_co8260103.1_g000001 Rmu_co8519155.1_g000001 Rmu_sc0000060.1_g000050 Rmu_sc0000255.1_g000036 Rmu_sc0001174.1_g000023 Rmu_sc0001174.1_g000025 Rmu_sc0001659.1_g000009 Rmu_sc0001706.1_g000009 Rmu_sc0001706.1_g000024 Rmu_sc0001706.1_g000035 Rmu_sc0004189.1_g000018 Rmu_sc0004189.1_g000030 Rmu_sc0004862.1_g000002 Rmu_sc0004876.1_g000038 Rmu_sc0023741.1_g000004
rosa_roxburghii Rroxscaffold_1G00075100 Rroxscaffold_1G00075160 Rroxscaffold_1G00075210 Rroxscaffold_5G00337160 Rroxscaffold_5G00386590 Rroxscaffold_6G00396300
rosa_rugosa Rorug03G0336200 Rorug03G0336300 Rorug04G0389400
rosa_samantha Rh2AG140400 Rh2BG144100 Rh2CG145400 Rh2DG144900 Rh3AG271200 Rh4AG038000 Rh4AG038700 Rh4BG032000 Rh4BG032800 Rh4CG041100 Rh4CG041300 Rh4DG035500 Rh4DG035600 Rh5AG008800 Rh5AG009100 Rh5BG010900 Rh5BG011500 Rh5BG011600 Rh5BG012000 Rh5CG009400 Rh5CG010000 Rh5DG009800
rosa_wichuraiana Rw2G010940 Rw4G002950 Rw4G002990 Rw4G003000 Rw5G000880 Rw5G000950 Rw5G000960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 394
AccBSI CCGCTC 1 cut(s) 670
AccI GTMKAC 2 cut(s) 1431, 2397
AccII CGCG 1 cut(s) 2287
AciI CCGC 8 cut(s) 93, 670, 1064, 1217, 1459, 1500, 1627, 1917
AclWI GGATC 9 cut(s) 283, 296, 1080, 1093, 1285, 1659, 1689, 1728, 1742
AcoI YGGCCR 1 cut(s) 94
AcsI RAATTY 3 cut(s) 744, 809, 1648
AcuI CTGAAG 4 cut(s) 73, 1247, 1281, 1711
AfaI GTAC 7 cut(s) 234, 322, 756, 2058, 2080, 2378, 2430
AfeI AGCGCT 2 cut(s) 664, 1751
AfiI CCNNNNNNNGG 3 cut(s) 291, 381, 2482
AflII CTTAAG 3 cut(s) 202, 1745, 1768
AflIII ACRYGT 1 cut(s) 2068
AjnI CCWGG 7 cut(s) 66, 131, 290, 1219, 2272, 2348, 2494
AjuI GAANNNNNNNTTGG 2 cut(s) 879, 911
AloI GAACNNNNNNTCC 4 cut(s) 32, 64, 2094, 2126
Alw21I GWGCWC 1 cut(s) 1977
Alw26I GTCTC 5 cut(s) 164, 333, 715, 2310, 2375
AlwI GGATC 9 cut(s) 283, 296, 1080, 1093, 1285, 1659, 1689, 1728, 1742
Aor51HI AGCGCT 2 cut(s) 664, 1751
AoxI GGCC 6 cut(s) 94, 135, 379, 562, 616, 2362
ApeKI GCWGC 5 cut(s) 188, 855, 858, 890, 1851
ApoI RAATTY 3 cut(s) 744, 809, 1648
ArsI GACNNNNNNTTYG 2 cut(s) 913, 945
Asp700I GAANNNNTTC 1 cut(s) 1957
AspA2I CCTAGG 1 cut(s) 1912
AspLEI GCGC 2 cut(s) 665, 1752
AspS9I GGNCC 5 cut(s) 25, 135, 379, 822, 1909
AsuC2I CCSGG 1 cut(s) 1007
AsuHPI GGTGA 2 cut(s) 121, 527
AvaII GGWCC 3 cut(s) 25, 822, 1909
AvrII CCTAGG 1 cut(s) 1912
AxyI CCTNAGG 1 cut(s) 1941
BaeGI GKGCMC 3 cut(s) 397, 1007, 1800
BamHI GGATCC 1 cut(s) 1085
BanI GGYRCC 1 cut(s) 394
BanII GRGCYC 2 cut(s) 1334, 1977
BbsI GAAGAC 3 cut(s) 119, 377, 1269
Bbv12I GWGCWC 1 cut(s) 1977
BbvI GCAGC 5 cut(s) 200, 845, 867, 877, 1863
BccI CCATC 5 cut(s) 266, 433, 1259, 1415, 1886
BciT130I CCWGG 7 cut(s) 68, 133, 292, 1221, 2274, 2350, 2496
BclI TGATCA 2 cut(s) 1282, 2043
BcnI CCSGG 1 cut(s) 1007
BcoDI GTCTC 5 cut(s) 164, 333, 715, 2310, 2375
BfmI CTRYAG 1 cut(s) 1080
BfoI RGCGCY 2 cut(s) 666, 1753
BfrI CTTAAG 3 cut(s) 202, 1745, 1768
BisI GCNGC 8 cut(s) 94, 189, 856, 859, 891, 1501, 1627, 1852
BlnI CCTAGG 1 cut(s) 1912
BlpI GCTNAGC 1 cut(s) 666
BlsI GCNGC 8 cut(s) 95, 190, 857, 860, 892, 1502, 1628, 1853
BmcAI AGTACT 3 cut(s) 756, 2058, 2378
Bme1390I CCNGG 8 cut(s) 68, 133, 292, 1007, 1221, 2274, 2350, 2496
Bme18I GGWCC 3 cut(s) 25, 822, 1909
BmgT120I GGNCC 5 cut(s) 25, 135, 379, 822, 1909
BmiI GGNNCC 4 cut(s) 289, 396, 1087, 2493
BmrFI CCNGG 8 cut(s) 68, 133, 292, 1007, 1221, 2274, 2350, 2496
BmrI ACTGGG 1 cut(s) 2272
BmuI ACTGGG 1 cut(s) 2272
BpiI GAAGAC 3 cut(s) 119, 377, 1269
BpmI CTGGAG 1 cut(s) 735
Bpu10I CCTNAGC 1 cut(s) 1601
Bpu1102I GCTNAGC 1 cut(s) 666
BpuEI CTTGAG 3 cut(s) 1318, 2048, 2357
BpuMI CCSGG 1 cut(s) 1007
BsaAI YACGTR 2 cut(s) 541, 1277
BsaI GGTCTC 1 cut(s) 2310
BsaJI CCNNGG 6 cut(s) 132, 375, 565, 1161, 1912, 2495
BsaWI WCCGGW 1 cut(s) 284
Bsc4I CCNNNNNNNGG 3 cut(s) 291, 381, 2482
Bse118I RCCGGY 1 cut(s) 381
Bse1I ACTGG 5 cut(s) 97, 458, 1301, 2267, 2405
Bse21I CCTNAGG 1 cut(s) 1941
Bse3DI GCAATG 2 cut(s) 579, 1238
BseBI CCWGG 7 cut(s) 68, 133, 292, 1221, 2274, 2350, 2496
BseDI CCNNGG 6 cut(s) 132, 375, 565, 1161, 1912, 2495
BseGI GGATG 5 cut(s) 277, 405, 684, 793, 1708
BseLI CCNNNNNNNGG 3 cut(s) 291, 381, 2482
BseMI GCAATG 2 cut(s) 579, 1238
BseNI ACTGG 5 cut(s) 97, 458, 1301, 2267, 2405
BseRI GAGGAG 6 cut(s) 694, 1423, 1505, 1586, 1808, 2196
BseSI GKGCMC 3 cut(s) 397, 1007, 1800
BseXI GCAGC 5 cut(s) 200, 845, 867, 877, 1863
BsgI GTGCAG 2 cut(s) 1424, 1678
Bsh1236I CGCG 1 cut(s) 2287
BshFI GGCC 6 cut(s) 96, 137, 381, 564, 618, 2364
BshNI GGYRCC 1 cut(s) 394
BsiHKAI GWGCWC 1 cut(s) 1977
BsiSI CCGG 4 cut(s) 285, 382, 1007, 2020
BslFI GGGAC 1 cut(s) 915
BslI CCNNNNNNNGG 3 cut(s) 291, 381, 2482
BsmAI GTCTC 5 cut(s) 164, 333, 715, 2310, 2375
BsmFI GGGAC 1 cut(s) 915
BsnI GGCC 6 cut(s) 96, 137, 381, 564, 618, 2364
Bso31I GGTCTC 1 cut(s) 2310
Bsp1286I GDGCHC 5 cut(s) 397, 1007, 1334, 1800, 1977
Bsp1720I GCTNAGC 1 cut(s) 666
Bsp68I TCGCGA 1 cut(s) 2287
BspACI CCGC 8 cut(s) 93, 670, 1064, 1217, 1459, 1500, 1627, 1917
BspANI GGCC 6 cut(s) 96, 137, 381, 564, 618, 2364
BspFNI CGCG 1 cut(s) 2287
BspHI TCATGA 1 cut(s) 598
BspLI GGNNCC 4 cut(s) 289, 396, 1087, 2493
BspPI GGATC 9 cut(s) 283, 296, 1080, 1093, 1285, 1659, 1689, 1728, 1742
BspT107I GGYRCC 1 cut(s) 394
BspTI CTTAAG 3 cut(s) 202, 1745, 1768
BspTNI GGTCTC 1 cut(s) 2310
BsrBI CCGCTC 1 cut(s) 670
BsrDI GCAATG 2 cut(s) 579, 1238
BsrFI RCCGGY 1 cut(s) 381
BsrI ACTGG 5 cut(s) 97, 458, 1301, 2267, 2405
BssAI RCCGGY 1 cut(s) 381
BssECI CCNNGG 6 cut(s) 132, 375, 565, 1161, 1912, 2495
BssT1I CCWWGG 3 cut(s) 375, 565, 1912
Bst2UI CCWGG 7 cut(s) 68, 133, 292, 1221, 2274, 2350, 2496
Bst4CI ACNGT 4 cut(s) 181, 929, 1699, 2086
Bst6I CTCTTC 3 cut(s) 1482, 1730, 1807
BstAFI CTTAAG 3 cut(s) 202, 1745, 1768
BstAPI GCANNNNNTGC 1 cut(s) 1237
BstBAI YACGTR 2 cut(s) 541, 1277
BstC8I GCNNGC 2 cut(s) 1068, 1238
BstF5I GGATG 5 cut(s) 277, 405, 684, 793, 1708
BstFNI CGCG 1 cut(s) 2287
BstH2I RGCGCY 2 cut(s) 666, 1753
BstHHI GCGC 2 cut(s) 665, 1752
BstMAI GTCTC 5 cut(s) 164, 333, 715, 2310, 2375
BstNI CCWGG 7 cut(s) 68, 133, 292, 1221, 2274, 2350, 2496
BstNSI RCATGY 1 cut(s) 2072
BstSCI CCNGG 8 cut(s) 66, 131, 290, 1005, 1219, 2272, 2348, 2494
BstSFI CTRYAG 1 cut(s) 1080
BstSLI GKGCMC 3 cut(s) 397, 1007, 1800
BstUI CGCG 1 cut(s) 2287
BstV1I GCAGC 5 cut(s) 200, 845, 867, 877, 1863
BstV2I GAAGAC 3 cut(s) 119, 377, 1269
BstX2I RGATCY 4 cut(s) 275, 1085, 1681, 1720
BstYI RGATCY 4 cut(s) 275, 1085, 1681, 1720
Bsu36I CCTNAGG 1 cut(s) 1941
BsuRI GGCC 6 cut(s) 96, 137, 381, 564, 618, 2364
BtgZI GCGATG 1 cut(s) 501
BtsCI GGATG 5 cut(s) 277, 405, 684, 793, 1708
BtsI GCAGTG 1 cut(s) 2341
BtsIMutI CAGTG 6 cut(s) 177, 237, 1127, 1294, 1695, 2341
BtuMI TCGCGA 1 cut(s) 2287
Cac8I GCNNGC 2 cut(s) 1068, 1238
CciI TCATGA 1 cut(s) 598
CfoI GCGC 2 cut(s) 665, 1752
Cfr10I RCCGGY 1 cut(s) 381
Cfr13I GGNCC 5 cut(s) 25, 135, 379, 822, 1909
Csp6I GTAC 7 cut(s) 233, 321, 755, 2057, 2079, 2377, 2429
CviAII CATG 6 cut(s) 556, 599, 784, 1148, 2069, 2440
CviQI GTAC 7 cut(s) 233, 321, 755, 2057, 2079, 2377, 2429
EaeI YGGCCR 1 cut(s) 94
Eam1104I CTCTTC 3 cut(s) 1482, 1730, 1807
EarI CTCTTC 3 cut(s) 1482, 1730, 1807
Ecl136II GAGCTC 1 cut(s) 1975
Eco130I CCWWGG 3 cut(s) 375, 565, 1912
Eco147I AGGCCT 2 cut(s) 564, 2364
Eco24I GRGCYC 2 cut(s) 1334, 1977
Eco31I GGTCTC 1 cut(s) 2310
Eco47I GGWCC 3 cut(s) 25, 822, 1909
Eco47III AGCGCT 2 cut(s) 664, 1751
Eco53kI GAGCTC 1 cut(s) 1975
Eco57I CTGAAG 4 cut(s) 73, 1247, 1281, 1711
Eco81I CCTNAGG 1 cut(s) 1941
EcoICRI GAGCTC 1 cut(s) 1975
EcoO109I RGGNCCY 1 cut(s) 135
EcoRI GAATTC 2 cut(s) 744, 1648
EcoRII CCWGG 7 cut(s) 66, 131, 290, 1219, 2272, 2348, 2494
EcoT14I CCWWGG 3 cut(s) 375, 565, 1912
EcoT22I ATGCAT 1 cut(s) 424
EcoT38I GRGCYC 2 cut(s) 1334, 1977
ErhI CCWWGG 3 cut(s) 375, 565, 1912
FaeI CATG 6 cut(s) 559, 602, 787, 1151, 2072, 2443
FalI AAGNNNNNCTT 2 cut(s) 1945, 1977
FaqI GGGAC 1 cut(s) 915
FatI CATG 6 cut(s) 555, 598, 783, 1147, 2068, 2439
FauI CCCGC 4 cut(s) 1071, 1224, 1452, 1910
FbaI TGATCA 2 cut(s) 1282, 2043
FblI GTMKAC 2 cut(s) 1431, 2397
Fnu4HI GCNGC 8 cut(s) 94, 189, 856, 859, 891, 1501, 1627, 1852
FokI GGATG 5 cut(s) 284, 392, 671, 800, 1715
FriOI GRGCYC 2 cut(s) 1334, 1977
Fsp4HI GCNGC 8 cut(s) 94, 189, 856, 859, 891, 1501, 1627, 1852
GlaI GCGC 2 cut(s) 664, 1751
GluI GCNGC 8 cut(s) 94, 189, 856, 859, 891, 1501, 1627, 1852
GsuI CTGGAG 1 cut(s) 735
HaeII RGCGCY 2 cut(s) 666, 1753
HaeIII GGCC 6 cut(s) 96, 137, 381, 564, 618, 2364
HapII CCGG 4 cut(s) 285, 382, 1007, 2020
HhaI GCGC 2 cut(s) 665, 1752
Hin1II CATG 6 cut(s) 559, 602, 787, 1151, 2072, 2443
Hin6I GCGC 2 cut(s) 663, 1750
HinP1I GCGC 2 cut(s) 663, 1750
HpaII CCGG 4 cut(s) 285, 382, 1007, 2020
HphI GGTGA 2 cut(s) 121, 527
Hpy166II GTNNAC 5 cut(s) 51, 822, 1058, 1432, 2398
Hpy188I TCNGA 4 cut(s) 732, 1227, 1673, 1764
Hpy8I GTNNAC 5 cut(s) 51, 822, 1058, 1432, 2398
HpyCH4III ACNGT 4 cut(s) 181, 929, 1699, 2086
HpyCH4IV ACGT 3 cut(s) 540, 1276, 1588
HpySE526I ACGT 3 cut(s) 540, 1276, 1588
Hsp92II CATG 6 cut(s) 559, 602, 787, 1151, 2072, 2443
HspAI GCGC 2 cut(s) 663, 1750
Ksp22I TGATCA 2 cut(s) 1282, 2043
LmnI GCTCC 5 cut(s) 40, 681, 716, 839, 1745
Lsp1109I GCAGC 5 cut(s) 200, 845, 867, 877, 1863
MaeII ACGT 3 cut(s) 540, 1276, 1588
MaeIII GTNAC 5 cut(s) 444, 528, 724, 868, 2456
MbiI CCGCTC 1 cut(s) 670
MfeI CAATTG 1 cut(s) 894
MflI RGATCY 4 cut(s) 275, 1085, 1681, 1720
MhlI GDGCHC 5 cut(s) 397, 1007, 1334, 1800, 1977
MlyI GAGTC 7 cut(s) 25, 166, 931, 1053, 1103, 1437, 2005
MmeI TCCRAC 5 cut(s) 459, 485, 488, 1659, 1852
Mph1103I ATGCAT 1 cut(s) 424
MroXI GAANNNNTTC 1 cut(s) 1957
MspA1I CMGCKG 3 cut(s) 93, 858, 1731
MspCI CTTAAG 3 cut(s) 202, 1745, 1768
MspI CCGG 4 cut(s) 285, 382, 1007, 2020
MspR9I CCNGG 8 cut(s) 68, 133, 292, 1007, 1221, 2274, 2350, 2496
MunI CAATTG 1 cut(s) 894
MvaI CCWGG 7 cut(s) 68, 133, 292, 1221, 2274, 2350, 2496
MvnI CGCG 1 cut(s) 2287
NciI CCSGG 1 cut(s) 1007
NlaIII CATG 6 cut(s) 559, 602, 787, 1151, 2072, 2443
NlaIV GGNNCC 4 cut(s) 289, 396, 1087, 2493
NmuCI GTSAC 1 cut(s) 444
NruI TCGCGA 1 cut(s) 2287
NsiI ATGCAT 1 cut(s) 424
NspI RCATGY 1 cut(s) 2072
PagI TCATGA 1 cut(s) 598
PceI AGGCCT 2 cut(s) 564, 2364
PciI ACATGT 1 cut(s) 2068
PcsI WCGNNNNNNNCGW 1 cut(s) 925
PdmI GAANNNNTTC 1 cut(s) 1957
PfeI GAWTC 5 cut(s) 457, 799, 921, 1983, 2353
PflFI GACNNNGTC 1 cut(s) 2321
PfoI TCCNGGA 1 cut(s) 2272
PkrI GCNGC 8 cut(s) 95, 190, 857, 860, 892, 1502, 1628, 1853
PleI GAGTC 7 cut(s) 25, 165, 931, 1053, 1103, 1436, 2004
PpsI GAGTC 7 cut(s) 25, 165, 931, 1053, 1103, 1436, 2004
Ppu21I YACGTR 2 cut(s) 541, 1277
PscI ACATGT 1 cut(s) 2068
Psp124BI GAGCTC 1 cut(s) 1977
Psp6I CCWGG 7 cut(s) 66, 131, 290, 1219, 2272, 2348, 2494
PspGI CCWGG 7 cut(s) 66, 131, 290, 1219, 2272, 2348, 2494
PspN4I GGNNCC 4 cut(s) 289, 396, 1087, 2493
PspPI GGNCC 5 cut(s) 25, 135, 379, 822, 1909
PsuI RGATCY 4 cut(s) 275, 1085, 1681, 1720
PsyI GACNNNGTC 1 cut(s) 2321
PvuII CAGCTG 2 cut(s) 858, 1731
RruI TCGCGA 1 cut(s) 2287
RsaI GTAC 7 cut(s) 234, 322, 756, 2058, 2080, 2378, 2430
RsaNI GTAC 7 cut(s) 233, 321, 755, 2057, 2079, 2377, 2429
SacI GAGCTC 1 cut(s) 1977
SatI GCNGC 8 cut(s) 94, 189, 856, 859, 891, 1501, 1627, 1852
Sau96I GGNCC 5 cut(s) 25, 135, 379, 822, 1909
ScaI AGTACT 3 cut(s) 756, 2058, 2378
SchI GAGTC 7 cut(s) 25, 166, 931, 1053, 1103, 1437, 2005
ScrFI CCNGG 8 cut(s) 68, 133, 292, 1007, 1221, 2274, 2350, 2496
SduI GDGCHC 5 cut(s) 397, 1007, 1334, 1800, 1977
SfcI CTRYAG 1 cut(s) 1080
SinI GGWCC 3 cut(s) 25, 822, 1909
SmlI CTYRAG 6 cut(s) 202, 1333, 1745, 1768, 2027, 2372
SmoI CTYRAG 6 cut(s) 202, 1333, 1745, 1768, 2027, 2372
SseBI AGGCCT 2 cut(s) 564, 2364
SsiI CCGC 8 cut(s) 93, 670, 1064, 1217, 1459, 1500, 1627, 1917
SstI GAGCTC 1 cut(s) 1977
StuI AGGCCT 2 cut(s) 564, 2364
StyD4I CCNGG 8 cut(s) 66, 131, 290, 1005, 1219, 2272, 2348, 2494
StyI CCWWGG 3 cut(s) 375, 565, 1912
TaaI ACNGT 4 cut(s) 181, 929, 1699, 2086
TaiI ACGT 3 cut(s) 543, 1279, 1591
TaqI TCGA 5 cut(s) 84, 516, 971, 1107, 2307
TatI WGTACW 5 cut(s) 232, 754, 2056, 2376, 2428
TauI GCSGC 3 cut(s) 96, 1503, 1629
TfiI GAWTC 5 cut(s) 457, 799, 921, 1983, 2353
TscAI CASTG 6 cut(s) 184, 244, 1127, 1301, 1702, 2341
TseFI GTSAC 1 cut(s) 444
TseI GCWGC 5 cut(s) 188, 855, 858, 890, 1851
Tsp45I GTSAC 1 cut(s) 444
TspGWI ACGGA 4 cut(s) 53, 167, 826, 2483
TspRI CASTG 6 cut(s) 184, 244, 1127, 1301, 1702, 2341
Tth111I GACNNNGTC 1 cut(s) 2321
Vha464I CTTAAG 3 cut(s) 202, 1745, 1768
VpaK11BI GGWCC 3 cut(s) 25, 822, 1909
XapI RAATTY 3 cut(s) 744, 809, 1648
XbaI TCTAGA 1 cut(s) 1135
XceI RCATGY 1 cut(s) 2072
XcmI CCANNNNNNNNNTGG 1 cut(s) 1301
XmaJI CCTAGG 1 cut(s) 1912
XmiI GTMKAC 2 cut(s) 1431, 2397
XmnI GAANNNNTTC 1 cut(s) 1957
ZrmI AGTACT 3 cut(s) 756, 2058, 2378
Zsp2I ATGCAT 1 cut(s) 424
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.