Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0000296

Physical Location & Seq

Genomic Coordinates Forward (+)

11243604 .. 11246153

Gene Structure 2550 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1122200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1656 bp

ATGGATGCATCACTCCCCATGGACAGTGAAGGACAAATGGTGGATGCTGGTGGTAAACGTGGTGGTTGGATCATCTTTTTATTCATCATAGGAACTTTAATGTGCTTGACACTTGCTGCTGGAGGATGGCAAGCAAATCTGGTTGTGTATCTGATTGAGGAGTTTAACGTGAAGAGCATTGATGCTACTCAGGTGTCAAATGTTGTCAATGGTTGCACTAGTTTATTTCCTATCATTGGAGCAATTGTTGCAGACTCTTTCTTTGGCTGTTTCCCTGTCATCCTGATCTCGTCTTGTGGATTGGTCCTATTCACTTTGACTGCAACTCTTGATTCCTTGAGGCCCCCTTCATGTGAGAATGGATCTAGCTTGTGCGAAACACCATCAAAAGTTCAGTTTTCAGTCCTATATGCTAGTATAACGCTGGGATCAATTGGACTGGGAGGCACTCGCTTTACTATAGCAACAATGGGAGCAAACCAATTTGACAAGCCTAAAAATCAAGGGATTTTCTTTAACTGGTTTTTCTTTGCCATGTATACGGCGACTGTTGTAAGTTCTACTGTCGTTGTGTATATTGAGGACAGTGTCAGTTGGGGGTTGGGATTTGGCCTATCTGCTCTTGTTAATTTGATTGGTCTGGTCGTATTCTCATCTGGAATCCATTTCTATCGTCGTGATAAGCCACAAGGTAGCCCATTTGTGAATATAGCTCGTGTTGTTGTTGCTAGTATCCGGAAATGGAATGTTAAGCTCACATCTCGAAGGGAGGATTACCACTACCACAATGATGGAGAGGCAAAGACAACTCCCGCAGCACCTAAAAAGAGTTTCAGATTCCTGAACAGCGCAGCCCTGGAAACAGAAGGGGACTTGAAGCCTGACGGCTCAATTGCAAGGCCATGGAGGTTGTGCACACTCCAGCAAGTAGAAGATCTCAAAACTATCATTAGAATTTTCCCCGTGTGGTCTTCCAGCATATTTATAAGTACCCCAATAGCGATTCAAATGAGCTTAACGGTCCTGCAAGCTCTAAAGATGGACCGTCACATTGGATCACATTTCAAAGTCCCGGTTGGATCTGTCCTAGTCGTGGTTACAATCGCCTCTTGCATCGCTGTCCCAATAATTGATCGTATCCTATATCCCGGCTGGCAGAAACTGACCCATAAATCTCCGACTCCCCTCCAAAGACTAGGAATTGGTCATGTCCTAAATATCACAGGCATGGCTGTTTCCGCATTGGTCGAGTCAAAGCGTCTCAAAACAGCTCACGGTTCCAAGGTGCCAATGTTGGTCATGTGGTTATTCCCACAGTTGGTTTTGGTGGGCATCGGTGAAGCATTTCATTTCCCGGGGGAACTTCAGTTTTGCTATCAAGAATTTCCAGCGTCACTTCGTACCACGGCGACTGCAATGGTTGCGATGATAATAGCCATTGCTTTTTACTTGAGCACGGCGCTGGTTGATCTTTTTCGGAGGGTTACAGAATGGCTGCCGGATGATATAAACGAAGGGAGGATAGACAATGTGTACTGGGTTGTGGTTGTGATAGGTGTGCTCAACTTTGGTTATTTTTTGTTATGTGCCAAGTTGTACAAGTATCAGAATGTTAAGGGAGTGGAAGATAGTAGCTCCTCAGTCTCTGAAGGTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0006811 GO:0006820 GO:0006821 GO:0006950 GO:0006970 GO:0008150 GO:0008509 GO:0009268 GO:0009605 GO:0009607 GO:0009624 GO:0009628 GO:0009651 GO:0009987 GO:0010447 GO:0010542 GO:0015075 GO:0015103 GO:0015108 GO:0015112 GO:0015291 GO:0015318 GO:0015513 GO:0015562 GO:0015698 GO:0015706 GO:0016020 GO:0022804 GO:0022857 GO:0033554 GO:0034220 GO:0042221 GO:0043207 GO:0044464 GO:0050896 GO:0051179 GO:0051234 GO:0051704 GO:0051707 GO:0051716 GO:0055085 GO:0070887 GO:0071214 GO:0071470 GO:0071472 GO:0071705 GO:0071944 GO:0098656 GO:0098660 GO:0098661 GO:0104004 GO:1902074 GO:1902075 GO:1902476

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PTR2

Protein Analysis

552

Amino Acids

60.37

Weight (kDa)

8.43

Isoelectric Point (pI)

42.25

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MFS_1	PF07690	35 - 482	1.5e-11	Major Facilitator Superfamily
PTR2	PF00854	95 - 497	4.5e-68	POT family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000296)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G45650 AT3G45650 AT3G45660 AT3G45660 AT3G45660 AT3G45680 AT3G45690 AT3G45700 AT3G45710 AT3G45720
fragaria_vesca	FvH4_3g33820 FvH4_3g33850 FvH4_3g33880 FvH4_3g33880 FvH4_3g33880 FvH4_3g33880 FvH4_3g33880 FvH4_3g33880 FvH4_3g33880 FvH4_3g33880 FvH4_3g45870 FvH4_7g20320 FvH4_7g20340 FvH4_7g20350 FvH4_7g20350
malus_domestica	MD01G1112600.v1.1 MD03G1108700.v1.1 MD03G1108900.v1.1 MD03G1109100.v1.1 MD03G1109300.v1.1 MD07G1180500.v1.1 MD11G1121600.v1.1 MD11G1122000.v1.1 MD11G1122200.v1.1 MD11G1122300.v1.1 MD11G1122500.v1.1
prunus_persica	Prupe.2G219800_v2.0.a1 Prupe.2G219800_v2.0.a1 Prupe.2G219900_v2.0.a1 Prupe.3G138100_v2.0.a1 Prupe.4G208900_v2.0.a1 Prupe.6G091800_v2.0.a1 Prupe.6G091800_v2.0.a1 Prupe.6G091900_v2.0.a1
pyrus_communis	pycom01g14110 pycom03g08880 pycom03g08900 pycom03g08910 pycom07g17370 pycom11g10350 pycom11g10360
rosa_chinensis	RchiOBHm_Chr1g0364271 RchiOBHm_Chr1g0364281 RchiOBHm_Chr1g0364291 RchiOBHm_Chr1g0364311 RchiOBHm_Chr5g0061391 RchiOBHm_Chr5g0061441
rosa_laevigata	RLG00000014217 RLG00000027496 RLG00000027497 RLG00000027498 RLG00000035463 RLG00000035473 RLG00000035475 RLG00000035477
rosa_multiflora	Rmu_co8497605.1_g000001 Rmu_sc0000671.1_g000002 Rmu_sc0003643.1_g000004 Rmu_sc0006608.1_g000010 Rmu_sc0006640.1_g000001 Rmu_sc0007470.1_g000001 Rmu_sc0015455.1_g000001 Rmu_sc0024335.1_g000001 Rmu_sc0031872.1_g000001 Rmu_sc0035434.1_g000001 Rmu_sc0035434.1_g000002
rosa_roxburghii	Rroxscaffold_1G00019240 Rroxscaffold_1G00019270 Rroxscaffold_1G00019280 Rroxscaffold_4G00292440 Rroxscaffold_4G00292450 Rroxscaffold_4G00292460 Rroxscaffold_4G00292470
rosa_rugosa	Rorug01G0315400 Rorug01G0315400 Rorug05G0340000 Rorug05G0340100 Rorug05G0340200 Rorug05G0340300
rosa_samantha	Rh1AG322600 Rh1AG322700 Rh1AG322800 Rh1AG322900 Rh1AG323000 Rh1BG285500 Rh1BG285600 Rh1CG302100 Rh1CG302200 Rh1CG302300 Rh1DG317000 Rh1DG317100 Rh5AG402200 Rh5AG402300 Rh5AG402400 Rh5AG402600 Rh5BG414600 Rh5BG414700 Rh5BG414900 Rh5BG415100 Rh5CG439300 Rh5CG439400 Rh5CG439600 Rh5CG439800 Rh5DG429100 Rh5DG429500 Rh5DG429700
rosa_wichuraiana	Rw1G028580 Rw1G028610 Rw1G028620 Rw5G037740 Rw5G037860 Rw5G037870

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	986
AccB1I	GGYRCC	1 cut(s)	1285
AccI	GTMKAC	1 cut(s)	539
AccIII	TCCGGA	1 cut(s)	735
AciI	CCGC	2 cut(s)	813, 1239
AclWI	GGATC	5 cut(s)	77, 370, 436, 1063, 1087
AcsI	RAATTY	2 cut(s)	954, 1382
AcuI	CTGAAG	1 cut(s)	1349
AfaI	GTAC	4 cut(s)	991, 1402, 1535, 1598
AfiI	CCNNNNNNNGG	3 cut(s)	236, 1093, 1318
AgsI	TTSAA	3 cut(s)	877, 1007, 1066
AhlI	ACTAGT	1 cut(s)	218
AjnI	CCWGG	1 cut(s)	855
AluBI	AGCT	7 cut(s)	369, 713, 754, 1014, 1031, 1271, 1635
AluI	AGCT	7 cut(s)	369, 713, 754, 1014, 1031, 1271, 1635
Alw21I	GWGCWC	3 cut(s)	917, 1457, 1563
Alw26I	GTCTC	2 cut(s)	1265, 1648
Alw44I	GTGCAC	1 cut(s)	913
AlwI	GGATC	5 cut(s)	77, 370, 436, 1063, 1087
AlwNI	CAGNNNCTG	2 cut(s)	1162, 1646
Ama87I	CYCGRG	1 cut(s)	1354
Aor13HI	TCCGGA	1 cut(s)	735
AoxI	GGCC	3 cut(s)	341, 610, 899
ApaLI	GTGCAC	1 cut(s)	913
ApeKI	GCWGC	4 cut(s)	116, 815, 851, 1495
ApoI	RAATTY	2 cut(s)	954, 1382
ArsI	GACNNNNNNTTYG	2 cut(s)	245, 277
Asp700I	GAANNNNTTC	1 cut(s)	1344
AspLEI	GCGC	2 cut(s)	851, 1462
AspS9I	GGNCC	4 cut(s)	304, 342, 1021, 1042
AsuC2I	CCSGG	4 cut(s)	1073, 1149, 1355, 1356
AsuHPI	GGTGA	1 cut(s)	1349
AvaI	CYCGRG	1 cut(s)	1354
AvaII	GGWCC	3 cut(s)	304, 1021, 1042
BaeGI	GKGCMC	1 cut(s)	917
BanI	GGYRCC	1 cut(s)	1285
BauI	CACGAG	1 cut(s)	714
BbsI	GAAGAC	1 cut(s)	963
Bbv12I	GWGCWC	3 cut(s)	917, 1457, 1563
BbvI	GCAGC	4 cut(s)	103, 827, 863, 1482
BccI	CCATC	4 cut(s)	120, 391, 785, 1033
BceAI	ACGGC	4 cut(s)	558, 901, 1422, 1473
BciT130I	CCWGG	1 cut(s)	857
BciVI	GTATCC	2 cut(s)	743, 1148
BcnI	CCSGG	4 cut(s)	1073, 1149, 1355, 1356
BcoDI	GTCTC	2 cut(s)	1265, 1648
BcuI	ACTAGT	1 cut(s)	218
BfaI	CTAG	6 cut(s)	219, 366, 414, 729, 1088, 1196
BfmI	CTRYAG	1 cut(s)	459
BfoI	RGCGCY	1 cut(s)	1463
BfuI	GTATCC	2 cut(s)	743, 1148
BglII	AGATCT	1 cut(s)	934
BisI	GCNGC	4 cut(s)	117, 816, 852, 1496
BlsI	GCNGC	4 cut(s)	118, 817, 853, 1497
Bme1390I	CCNGG	5 cut(s)	857, 1073, 1149, 1355, 1356
Bme18I	GGWCC	3 cut(s)	304, 1021, 1042
BmeT110I	CYCGRG	1 cut(s)	1354
BmgT120I	GGNCC	4 cut(s)	304, 342, 1021, 1042
BmiI	GGNNCC	3 cut(s)	344, 1279, 1287
BmrFI	CCNGG	5 cut(s)	857, 1073, 1149, 1355, 1356
BmrI	ACTGGG	2 cut(s)	449, 1546
BmsI	GCATC	5 cut(s)	17, 34, 172, 1122, 1341
BmuI	ACTGGG	2 cut(s)	449, 1546
BpiI	GAAGAC	1 cut(s)	963
BpmI	CTGGAG	2 cut(s)	141, 905
BpuEI	CTTGAG	2 cut(s)	358, 1471
BpuMI	CCSGG	4 cut(s)	1073, 1149, 1355, 1356
BsaJI	CCNNGG	7 cut(s)	18, 855, 902, 1281, 1354, 1355, 1404
BsaWI	WCCGGW	1 cut(s)	735
BsaXI	ACNNNNNCTCC	2 cut(s)	231, 261
Bsc4I	CCNNNNNNNGG	3 cut(s)	236, 1093, 1318
Bse1I	ACTGG	3 cut(s)	444, 524, 1541
Bse3DI	GCAATG	2 cut(s)	1422, 1437
BseAI	TCCGGA	1 cut(s)	735
BseBI	CCWGG	1 cut(s)	857
BseDI	CCNNGG	7 cut(s)	18, 855, 902, 1281, 1354, 1355, 1404
BseGI	GGATG	5 cut(s)	10, 49, 131, 279, 1507
BseLI	CCNNNNNNNGG	3 cut(s)	236, 1093, 1318
BseMI	GCAATG	2 cut(s)	1422, 1437
BseMII	CTCAG	2 cut(s)	203, 1653
BseNI	ACTGG	3 cut(s)	444, 524, 1541
BseRI	GAGGAG	2 cut(s)	173, 1627
BseSI	GKGCMC	1 cut(s)	917
BseXI	GCAGC	4 cut(s)	103, 827, 863, 1482
BseYI	CCCAGC	1 cut(s)	424
BshFI	GGCC	3 cut(s)	343, 612, 901
BshNI	GGYRCC	1 cut(s)	1285
BsiHKAI	GWGCWC	3 cut(s)	917, 1457, 1563
BsiHKCI	CYCGRG	1 cut(s)	1354
BsiSI	CCGG	5 cut(s)	736, 1073, 1149, 1355, 1499
BslFI	GGGAC	3 cut(s)	884, 1055, 1106
BslI	CCNNNNNNNGG	3 cut(s)	236, 1093, 1318
BsmAI	GTCTC	2 cut(s)	1265, 1648
BsmBI	CGTCTC	1 cut(s)	1265
BsmFI	GGGAC	3 cut(s)	884, 1055, 1106
BsnI	GGCC	3 cut(s)	343, 612, 901
BsoBI	CYCGRG	1 cut(s)	1354
Bsp1286I	GDGCHC	3 cut(s)	917, 1457, 1563
Bsp13I	TCCGGA	1 cut(s)	735
Bsp1407I	TGTACA	1 cut(s)	1596
Bsp143I	GATC	9 cut(s)	69, 285, 362, 428, 934, 1055, 1079, 1132, 1468
Bsp19I	CCATGG	2 cut(s)	18, 902
BspACI	CCGC	2 cut(s)	813, 1239
BspANI	GGCC	3 cut(s)	343, 612, 901
BspCNI	CTCAG	2 cut(s)	202, 1652
BspEI	TCCGGA	1 cut(s)	735
BspLI	GGNNCC	3 cut(s)	344, 1279, 1287
BspPI	GGATC	5 cut(s)	77, 370, 436, 1063, 1087
BspQI	GCTCTTC	1 cut(s)	167
BspT107I	GGYRCC	1 cut(s)	1285
BsrDI	GCAATG	2 cut(s)	1422, 1437
BsrGI	TGTACA	1 cut(s)	1596
BsrI	ACTGG	3 cut(s)	444, 524, 1541
BssECI	CCNNGG	7 cut(s)	18, 855, 902, 1281, 1354, 1355, 1404
BssMI	GATC	9 cut(s)	69, 285, 362, 428, 934, 1055, 1079, 1132, 1468
BssNAI	GTATAC	1 cut(s)	540
BssSI	CACGAG	1 cut(s)	714
BssT1I	CCWWGG	3 cut(s)	18, 902, 1281
Bst1107I	GTATAC	1 cut(s)	540
Bst2BI	CACGAG	1 cut(s)	714
Bst2UI	CCWGG	1 cut(s)	857
Bst4CI	ACNGT	8 cut(s)	26, 550, 565, 587, 1021, 1046, 1277, 1317
Bst6I	CTCTTC	1 cut(s)	167
BstAPI	GCANNNNNTGC	2 cut(s)	248, 1421
BstAUI	TGTACA	1 cut(s)	1596
BstC8I	GCNNGC	3 cut(s)	132, 1029, 1154
BstDEI	CTNAG	2 cut(s)	189, 1639
BstDSI	CCRYGG	3 cut(s)	18, 902, 1404
BstF5I	GGATG	5 cut(s)	10, 49, 131, 279, 1507
BstH2I	RGCGCY	1 cut(s)	1463
BstHHI	GCGC	2 cut(s)	851, 1462
BstKTI	GATC	9 cut(s)	72, 288, 365, 431, 937, 1058, 1082, 1135, 1471
BstMAI	GTCTC	2 cut(s)	1265, 1648
BstMBI	GATC	9 cut(s)	69, 285, 362, 428, 934, 1055, 1079, 1132, 1468
BstMWI	GCNNNNNNNGC	3 cut(s)	248, 1238, 1421
BstNI	CCWGG	1 cut(s)	857
BstSCI	CCNGG	5 cut(s)	855, 1071, 1147, 1353, 1354
BstSFI	CTRYAG	1 cut(s)	459
BstSLI	GKGCMC	1 cut(s)	917
BstV1I	GCAGC	4 cut(s)	103, 827, 863, 1482
BstV2I	GAAGAC	1 cut(s)	963
BstX2I	RGATCY	3 cut(s)	362, 934, 1079
BstXI	CCANNNNNNTGG	1 cut(s)	791
BstYI	RGATCY	3 cut(s)	362, 934, 1079
BstZ17I	GTATAC	1 cut(s)	540
BsuI	GTATCC	2 cut(s)	743, 1148
BsuRI	GGCC	3 cut(s)	343, 612, 901
BtgI	CCRYGG	3 cut(s)	18, 902, 1404
BtgZI	GCGATG	2 cut(s)	1099, 1439
BtsCI	GGATG	5 cut(s)	10, 49, 131, 279, 1507
BtsIMutI	CAGTG	2 cut(s)	31, 592
Cac8I	GCNNGC	3 cut(s)	132, 1029, 1154
CaiI	CAGNNNCTG	2 cut(s)	1162, 1646
CfoI	GCGC	2 cut(s)	851, 1462
Cfr13I	GGNCC	4 cut(s)	304, 342, 1021, 1042
Cfr9I	CCCGGG	1 cut(s)	1354
CseI	GACGC	2 cut(s)	1247, 1380
Csp6I	GTAC	4 cut(s)	990, 1401, 1534, 1597
CviAII	CATG	7 cut(s)	19, 351, 535, 903, 1208, 1228, 1300
CviQI	GTAC	4 cut(s)	990, 1401, 1534, 1597
DdeI	CTNAG	2 cut(s)	189, 1639
DpnI	GATC	9 cut(s)	71, 287, 364, 430, 936, 1057, 1081, 1134, 1470
DpnII	GATC	9 cut(s)	69, 285, 362, 428, 934, 1055, 1079, 1132, 1468
Eam1104I	CTCTTC	1 cut(s)	167
EarI	CTCTTC	1 cut(s)	167
Eco130I	CCWWGG	3 cut(s)	18, 902, 1281
Eco47I	GGWCC	3 cut(s)	304, 1021, 1042
Eco57I	CTGAAG	1 cut(s)	1349
Eco88I	CYCGRG	1 cut(s)	1354
EcoO109I	RGGNCCY	1 cut(s)	342
EcoRII	CCWGG	1 cut(s)	855
EcoT14I	CCWWGG	3 cut(s)	18, 902, 1281
EcoT22I	ATGCAT	1 cut(s)	10
ErhI	CCWWGG	3 cut(s)	18, 902, 1281
Esp3I	CGTCTC	1 cut(s)	1265
FaeI	CATG	7 cut(s)	22, 354, 538, 906, 1211, 1231, 1303
FaqI	GGGAC	3 cut(s)	884, 1055, 1106
FatI	CATG	7 cut(s)	18, 350, 534, 902, 1207, 1227, 1299
FauI	CCCGC	1 cut(s)	820
FblI	GTMKAC	1 cut(s)	539
Fnu4HI	GCNGC	4 cut(s)	117, 816, 852, 1496
FokI	GGATG	5 cut(s)	17, 56, 138, 266, 1514
Fsp4HI	GCNGC	4 cut(s)	117, 816, 852, 1496
FspBI	CTAG	6 cut(s)	219, 366, 414, 729, 1088, 1196
GlaI	GCGC	2 cut(s)	850, 1461
GluI	GCNGC	4 cut(s)	117, 816, 852, 1496
GsaI	CCCAGC	1 cut(s)	428
GsuI	CTGGAG	2 cut(s)	141, 905
HaeII	RGCGCY	1 cut(s)	1463
HaeIII	GGCC	3 cut(s)	343, 612, 901
HapII	CCGG	5 cut(s)	736, 1073, 1149, 1355, 1499
HgaI	GACGC	2 cut(s)	1247, 1380
HhaI	GCGC	2 cut(s)	851, 1462
Hin1II	CATG	7 cut(s)	22, 354, 538, 906, 1211, 1231, 1303
Hin6I	GCGC	2 cut(s)	849, 1460
HinP1I	GCGC	2 cut(s)	849, 1460
HinfI	GANTC	7 cut(s)	254, 332, 660, 837, 1003, 1180, 1250
HpaII	CCGG	5 cut(s)	736, 1073, 1149, 1355, 1499
HphI	GGTGA	1 cut(s)	1349
Hpy166II	GTNNAC	4 cut(s)	56, 540, 915, 1534
Hpy188I	TCNGA	6 cut(s)	153, 836, 1179, 1479, 1608, 1648
Hpy188III	TCNNGA	8 cut(s)	283, 329, 657, 677, 736, 762, 841, 1379
Hpy8I	GTNNAC	4 cut(s)	56, 540, 915, 1534
Hpy99I	CGWCG	1 cut(s)	678
HpyAV	CCTTC	6 cut(s)	23, 357, 759, 860, 1508, 1643
HpyCH4III	ACNGT	8 cut(s)	26, 550, 565, 587, 1021, 1046, 1277, 1317
HpyCH4IV	ACGT	2 cut(s)	58, 168
HpyCH4V	TGCA	9 cut(s)	8, 216, 251, 323, 896, 915, 1027, 1113, 1415
HpyF10VI	GCNNNNNNNGC	3 cut(s)	248, 1238, 1421
HpyF3I	CTNAG	2 cut(s)	189, 1639
HpySE526I	ACGT	2 cut(s)	58, 168
Hsp92II	CATG	7 cut(s)	22, 354, 538, 906, 1211, 1231, 1303
HspAI	GCGC	2 cut(s)	849, 1460
Kpn2I	TCCGGA	1 cut(s)	735
Kzo9I	GATC	9 cut(s)	69, 285, 362, 428, 934, 1055, 1079, 1132, 1468
LguI	GCTCTTC	1 cut(s)	167
LmnI	GCTCC	3 cut(s)	239, 473, 1640
Lsp1109I	GCAGC	4 cut(s)	103, 827, 863, 1482
LweI	GCATC	5 cut(s)	17, 34, 172, 1122, 1341
MaeI	CTAG	6 cut(s)	219, 366, 414, 729, 1088, 1196
MaeII	ACGT	2 cut(s)	58, 168
MaeIII	GTNAC	4 cut(s)	1046, 1096, 1392, 1483
MalI	GATC	9 cut(s)	71, 287, 364, 430, 936, 1057, 1081, 1134, 1470
MboI	GATC	9 cut(s)	69, 285, 362, 428, 934, 1055, 1079, 1132, 1468
MboII	GAAGA	4 cut(s)	184, 944, 963, 1637
MfeI	CAATTG	3 cut(s)	243, 432, 891
MflI	RGATCY	3 cut(s)	362, 934, 1079
MhlI	GDGCHC	3 cut(s)	917, 1457, 1563
MluCI	AATT	9 cut(s)	243, 432, 482, 628, 891, 954, 1128, 1200, 1382
MlyI	GAGTC	3 cut(s)	248, 1174, 1259
MmeI	TCCRAC	3 cut(s)	47, 1057, 1202
Mph1103I	ATGCAT	1 cut(s)	10
MroI	TCCGGA	1 cut(s)	735
MroXI	GAANNNNTTC	1 cut(s)	1344
MseI	TTAA	7 cut(s)	98, 165, 516, 627, 750, 1016, 1614
MslI	CAYNNNNRTG	2 cut(s)	789, 1226
MspI	CCGG	5 cut(s)	736, 1073, 1149, 1355, 1499
MspR9I	CCNGG	5 cut(s)	857, 1073, 1149, 1355, 1356
MunI	CAATTG	3 cut(s)	243, 432, 891
MvaI	CCWGG	1 cut(s)	857
MwoI	GCNNNNNNNGC	3 cut(s)	248, 1238, 1421
NciI	CCSGG	4 cut(s)	1073, 1149, 1355, 1356
NcoI	CCATGG	2 cut(s)	18, 902
NdeII	GATC	9 cut(s)	69, 285, 362, 428, 934, 1055, 1079, 1132, 1468
NlaIII	CATG	7 cut(s)	22, 354, 538, 906, 1211, 1231, 1303
NlaIV	GGNNCC	3 cut(s)	344, 1279, 1287
NmuCI	GTSAC	2 cut(s)	1046, 1392
NsiI	ATGCAT	1 cut(s)	10
PciSI	GCTCTTC	1 cut(s)	167
PdmI	GAANNNNTTC	1 cut(s)	1344
PfeI	GAWTC	4 cut(s)	332, 660, 837, 1003
PflFI	GACNNNGTC	1 cut(s)	587
PkrI	GCNGC	4 cut(s)	118, 817, 853, 1497
PleI	GAGTC	3 cut(s)	248, 1174, 1258
PpsI	GAGTC	3 cut(s)	248, 1174, 1258
PsiI	TTATAA	1 cut(s)	986
Psp6I	CCWGG	1 cut(s)	855
PspFI	CCCAGC	1 cut(s)	424
PspGI	CCWGG	1 cut(s)	855
PspN4I	GGNNCC	3 cut(s)	344, 1279, 1287
PspPI	GGNCC	4 cut(s)	304, 342, 1021, 1042
PstNI	CAGNNNCTG	2 cut(s)	1162, 1646
PsuI	RGATCY	3 cut(s)	362, 934, 1079
PsyI	GACNNNGTC	1 cut(s)	587
RsaI	GTAC	4 cut(s)	991, 1402, 1535, 1598
RsaNI	GTAC	4 cut(s)	990, 1401, 1534, 1597
RseI	CAYNNNNRTG	2 cut(s)	789, 1226
SapI	GCTCTTC	1 cut(s)	167
SaqAI	TTAA	7 cut(s)	98, 165, 516, 627, 750, 1016, 1614
SatI	GCNGC	4 cut(s)	117, 816, 852, 1496
Sau3AI	GATC	9 cut(s)	69, 285, 362, 428, 934, 1055, 1079, 1132, 1468
Sau96I	GGNCC	4 cut(s)	304, 342, 1021, 1042
SchI	GAGTC	3 cut(s)	248, 1174, 1259
ScrFI	CCNGG	5 cut(s)	857, 1073, 1149, 1355, 1356
SduI	GDGCHC	3 cut(s)	917, 1457, 1563
SfaNI	GCATC	5 cut(s)	17, 34, 172, 1122, 1341
SfcI	CTRYAG	1 cut(s)	459
SinI	GGWCC	3 cut(s)	304, 1021, 1042
SmaI	CCCGGG	1 cut(s)	1356
SmiMI	CAYNNNNRTG	2 cut(s)	789, 1226
SmlI	CTYRAG	2 cut(s)	337, 1450
SmoI	CTYRAG	2 cut(s)	337, 1450
SpeI	ACTAGT	1 cut(s)	218
Sse9I	AATT	9 cut(s)	243, 432, 482, 628, 891, 954, 1128, 1200, 1382
SsiI	CCGC	2 cut(s)	813, 1239
SspMI	CTAG	6 cut(s)	219, 366, 414, 729, 1088, 1196
StyD4I	CCNGG	5 cut(s)	855, 1071, 1147, 1353, 1354
StyI	CCWWGG	3 cut(s)	18, 902, 1281
TaaI	ACNGT	8 cut(s)	26, 550, 565, 587, 1021, 1046, 1277, 1317
TaiI	ACGT	2 cut(s)	61, 171
TaqI	TCGA	2 cut(s)	763, 1248
TasI	AATT	9 cut(s)	243, 432, 482, 628, 891, 954, 1128, 1200, 1382
TatI	WGTACW	2 cut(s)	1533, 1596
TfiI	GAWTC	4 cut(s)	332, 660, 837, 1003
Tru1I	TTAA	7 cut(s)	98, 165, 516, 627, 750, 1016, 1614
Tru9I	TTAA	7 cut(s)	98, 165, 516, 627, 750, 1016, 1614
TscAI	CASTG	2 cut(s)	31, 592
TseFI	GTSAC	2 cut(s)	1046, 1392
TseI	GCWGC	4 cut(s)	116, 815, 851, 1495
Tsp45I	GTSAC	2 cut(s)	1046, 1392
TspDTI	ATGAA	3 cut(s)	73, 339, 1337
TspMI	CCCGGG	1 cut(s)	1354
TspRI	CASTG	2 cut(s)	31, 592
Tth111I	GACNNNGTC	1 cut(s)	587
VneI	GTGCAC	1 cut(s)	913
VpaK11BI	GGWCC	3 cut(s)	304, 1021, 1042
XapI	RAATTY	2 cut(s)	954, 1382
XmaI	CCCGGG	1 cut(s)	1354
XmiI	GTMKAC	1 cut(s)	539
XmnI	GAANNNNTTC	1 cut(s)	1344
XspI	CTAG	6 cut(s)	219, 366, 414, 729, 1088, 1196
Zsp2I	ATGCAT	1 cut(s)	10

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.