Rroxscaffold_4G00292450

Protein NRT1 PTR FAMILY

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000296
Physical Location & Seq
Forward (+)
12752030 .. 12762641
10612 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00292450.1

Sequence Viewer

Length: 1344 bp
ATGTTGACCCTAATAGCAATTATCCCTTCCTTGAGGCCCTCACCATGTTCAATTGGCTCACTTATGACATGTCAAGCTCCATCAAAGCTTCAATACTCAGTCCTATATGCAGCATTATCACTAGTTTCACTAGGACTTGGAGGCACCCGCTTCACAATTGCAACCATGGGAGCAGACCAATTCAAAAATCCGAATGATCAAGAGGTTTTCTTTAATTGGTATTTTGTGGCTTTGTATATGTCCAGTGTCTTTAGCGGCACTGCCATCGTATATATTCAGGACAACGTGGGATGGGGATTAGGGTTTGGAATTTGCTGCATTGCAAATGGTCTGGGATTGGGGGTGTTCTTATTGGGGAAACGATTTTATGAGCAAGTAAAACCAAAGGGGAGTCCATTCTTGGGCATAGCACGAGTATTGGTTGCAGCAATTCGGAAGAGGAAGATGTCATCAGAAACTTCAACAGAGCCTGATTACTATTATGGCGCAGGAGCAATGACTGACAATCCACCAACTAAAAGTATCAGATTCCTGAACTGTGCAGCCTTGAAAAGTGAACAAGACAAACAATTTGTAGGCTCGTATGGAAACAGATGGAGGCTATGCACTGTGAAAGAAGTAGAAGACCTCAAAACCCTAATTAAGATCATGCCGCTGTGGTCTACTGGCATCTTCCTAAGCACACCAATAGGCATTTTAAGCAGCCTCACTATACTCCAAGCCCTAACCATGGATAGACACCTTGGTCCACACTTCAAAATCCCTGCTTCTTCATTCCTAGTGTTCGCCCTCTTAGCCACAGCCATCTCCATCTTTATAATTGACCGCTTCATACTCCTCAAGTACAAGAATCTCACTGGACGAACTTTAACACCGACCCAGCGAATAGGGATCGGTCACATCATCAACATTATTTGTCTAATTGGGGCTGCCCTAATCGAAAGAAGACGACTTGGAGTGGCAAGATCACATAGCCTTATGAACCAACCTGGTTCAGAGGTGCCCATGTCCGCCTTTTGGCTTGTGGCACCGTTATCTGTTATGGGAATCGGTGAGGCATTTCATTTCCCTGGACAAGTTTCGTTGTGCTATCAAGAATTTCCAAAATCATTGAGAAGTACATCCACTGCAATGATCTCATTATTACTTGGAATCGGGTATTATCTTAGCACTGCAGTCACTGATTTGGTAGACAAGGCTACGAATTGGTTGCCAGATGATATAAATCAAGGGCGGTTGGATAATGTCTTTTGGATGTTGGTAGTGATTGGGGTGGTGAATTTTGGTTACTACATAACTTGTGCTAAGTTTTTTGTGTCCCAAAAACAAAATGTTGGGCAATAA

Protein Analysis

447

Amino Acids

49.18

Weight (kDa)

9.36

Isoelectric Point (pI)

37.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTR2 PF00854 2 - 403 1.3e-64 POT family
MFS_1 PF07690 32 - 387 5e-09 Major Facilitator Superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000296)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G45650 AT3G45650 AT3G45660 AT3G45660 AT3G45660 AT3G45680 AT3G45690 AT3G45700 AT3G45710 AT3G45720
fragaria_vesca FvH4_3g33820 FvH4_3g33850 FvH4_3g33880 FvH4_3g33880 FvH4_3g33880 FvH4_3g33880 FvH4_3g33880 FvH4_3g33880 FvH4_3g33880 FvH4_3g33880 FvH4_3g45870 FvH4_7g20320 FvH4_7g20340 FvH4_7g20350 FvH4_7g20350
malus_domestica MD01G1112600.v1.1 MD03G1108700.v1.1 MD03G1108900.v1.1 MD03G1109100.v1.1 MD03G1109300.v1.1 MD07G1180500.v1.1 MD11G1121600.v1.1 MD11G1122000.v1.1 MD11G1122200.v1.1 MD11G1122300.v1.1 MD11G1122500.v1.1
prunus_persica Prupe.2G219800_v2.0.a1 Prupe.2G219800_v2.0.a1 Prupe.2G219900_v2.0.a1 Prupe.3G138100_v2.0.a1 Prupe.4G208900_v2.0.a1 Prupe.6G091800_v2.0.a1 Prupe.6G091800_v2.0.a1 Prupe.6G091900_v2.0.a1
pyrus_communis pycom01g14110 pycom03g08880 pycom03g08900 pycom03g08910 pycom07g17370 pycom11g10350 pycom11g10360
rosa_chinensis RchiOBHm_Chr1g0364271 RchiOBHm_Chr1g0364281 RchiOBHm_Chr1g0364291 RchiOBHm_Chr1g0364311 RchiOBHm_Chr5g0061391 RchiOBHm_Chr5g0061441
rosa_laevigata RLG00000014217 RLG00000027496 RLG00000027497 RLG00000027498 RLG00000035463 RLG00000035473 RLG00000035475 RLG00000035477
rosa_multiflora Rmu_co8497605.1_g000001 Rmu_sc0000671.1_g000002 Rmu_sc0003643.1_g000004 Rmu_sc0006608.1_g000010 Rmu_sc0006640.1_g000001 Rmu_sc0007470.1_g000001 Rmu_sc0015455.1_g000001 Rmu_sc0024335.1_g000001 Rmu_sc0031872.1_g000001 Rmu_sc0035434.1_g000001 Rmu_sc0035434.1_g000002
rosa_roxburghii Rroxscaffold_1G00019240 Rroxscaffold_1G00019270 Rroxscaffold_1G00019280 Rroxscaffold_4G00292440 Rroxscaffold_4G00292450 Rroxscaffold_4G00292460 Rroxscaffold_4G00292470
rosa_rugosa Rorug01G0315400 Rorug01G0315400 Rorug05G0340000 Rorug05G0340100 Rorug05G0340200 Rorug05G0340300
rosa_samantha Rh1AG322600 Rh1AG322700 Rh1AG322800 Rh1AG322900 Rh1AG323000 Rh1BG285500 Rh1BG285600 Rh1CG302100 Rh1CG302200 Rh1CG302300 Rh1DG317000 Rh1DG317100 Rh5AG402200 Rh5AG402300 Rh5AG402400 Rh5AG402600 Rh5BG414600 Rh5BG414700 Rh5BG414900 Rh5BG415100 Rh5CG439300 Rh5CG439400 Rh5CG439600 Rh5CG439800 Rh5DG429100 Rh5DG429500 Rh5DG429700
rosa_wichuraiana Rw1G028580 Rw1G028610 Rw1G028620 Rw5G037740 Rw5G037860 Rw5G037870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 818
AasI GACNNNNNNGTC 1 cut(s) 744
AccB1I GGYRCC 3 cut(s) 143, 1000, 1027
AccI GTMKAC 2 cut(s) 662, 1191
AciI CCGC 6 cut(s) 148, 255, 653, 826, 1011, 1234
AclWI GGATC 1 cut(s) 899
AcsI RAATTY 3 cut(s) 309, 1097, 1279
AfaI GTAC 2 cut(s) 845, 1120
AfiI CCNNNNNNNGG 3 cut(s) 401, 730, 1017
AflIII ACRYGT 1 cut(s) 68
AgsI TTSAA 6 cut(s) 51, 92, 184, 462, 550, 757
AhlI ACTAGT 1 cut(s) 121
AjnI CCWGG 2 cut(s) 988, 1069
AluBI AGCT 2 cut(s) 77, 88
AluI AGCT 2 cut(s) 77, 88
AlwI GGATC 1 cut(s) 899
AlwNI CAGNNNCTG 2 cut(s) 470, 1181
AoxI GGCC 1 cut(s) 35
ApeKI GCWGC 6 cut(s) 110, 315, 425, 542, 702, 929
ApoI RAATTY 3 cut(s) 309, 1097, 1279
ArsI GACNNNNNNTTYG 2 cut(s) 554, 586
AspLEI GCGC 1 cut(s) 488
AspS9I GGNCC 2 cut(s) 36, 746
AsuHPI GGTGA 3 cut(s) 33, 1064, 1288
AvaII GGWCC 1 cut(s) 746
BaeGI GKGCMC 1 cut(s) 1005
BanI GGYRCC 3 cut(s) 143, 1000, 1027
BauI CACGAG 1 cut(s) 411
BbsI GAAGAC 2 cut(s) 630, 952
BbvI GCAGC 6 cut(s) 122, 302, 437, 554, 714, 916
BccI CCATC 6 cut(s) 88, 272, 285, 588, 812, 818
BcgI CGANNNNNNTGC 4 cut(s) 247, 281, 1192, 1226
BciT130I CCWGG 2 cut(s) 990, 1071
BclI TGATCA 1 cut(s) 196
BcuI ACTAGT 1 cut(s) 121
BfaI CTAG 3 cut(s) 122, 131, 779
BfmI CTRYAG 1 cut(s) 1173
BisI GCNGC 8 cut(s) 111, 256, 316, 426, 543, 653, 703, 930
BlsI GCNGC 8 cut(s) 112, 257, 317, 427, 544, 654, 704, 931
Bme1390I CCNGG 2 cut(s) 990, 1071
Bme18I GGWCC 1 cut(s) 746
BmgT120I GGNCC 2 cut(s) 36, 746
BmiI GGNNCC 3 cut(s) 145, 1002, 1029
BmrFI CCNGG 2 cut(s) 990, 1071
BmsI GCATC 1 cut(s) 678
BpiI GAAGAC 2 cut(s) 630, 952
Bpu10I CCTNAGC 1 cut(s) 677
BpuEI CTTGAG 2 cut(s) 52, 824
BsaBI GATNNNNATC 1 cut(s) 1224
BsaJI CCNNGG 4 cut(s) 165, 729, 742, 1069
Bsc4I CCNNNNNNNGG 3 cut(s) 401, 730, 1017
Bse1I ACTGG 3 cut(s) 243, 670, 862
Bse3DI GCAATG 3 cut(s) 318, 501, 1137
Bse8I GATNNNNATC 1 cut(s) 1224
BseBI CCWGG 2 cut(s) 990, 1071
BseDI CCNNGG 4 cut(s) 165, 729, 742, 1069
BseGI GGATG 3 cut(s) 296, 1121, 1260
BseJI GATNNNNATC 1 cut(s) 1224
BseLI CCNNNNNNNGG 3 cut(s) 401, 730, 1017
BseMI GCAATG 3 cut(s) 318, 501, 1137
BseMII CTCAG 1 cut(s) 111
BseNI ACTGG 3 cut(s) 243, 670, 862
BseRI GAGGAG 1 cut(s) 827
BseSI GKGCMC 1 cut(s) 1005
BseXI GCAGC 6 cut(s) 122, 302, 437, 554, 714, 916
BseYI CCCAGC 1 cut(s) 879
BsgI GTGCAG 1 cut(s) 561
BshFI GGCC 1 cut(s) 37
BshNI GGYRCC 3 cut(s) 143, 1000, 1027
BslFI GGGAC 1 cut(s) 1303
BslI CCNNNNNNNGG 3 cut(s) 401, 730, 1017
BsmFI GGGAC 1 cut(s) 1303
BsnI GGCC 1 cut(s) 37
Bsp1286I GDGCHC 1 cut(s) 1005
Bsp143I GATC 5 cut(s) 196, 645, 891, 965, 1134
Bsp19I CCATGG 2 cut(s) 165, 729
BspACI CCGC 6 cut(s) 148, 255, 653, 826, 1011, 1234
BspANI GGCC 1 cut(s) 37
BspCNI CTCAG 1 cut(s) 110
BspLI GGNNCC 3 cut(s) 145, 1002, 1029
BspMAI CTGCAG 1 cut(s) 1177
BspPI GGATC 1 cut(s) 899
BspT107I GGYRCC 3 cut(s) 143, 1000, 1027
BsrDI GCAATG 3 cut(s) 318, 501, 1137
BsrI ACTGG 3 cut(s) 243, 670, 862
BssECI CCNNGG 4 cut(s) 165, 729, 742, 1069
BssMI GATC 5 cut(s) 196, 645, 891, 965, 1134
BssSI CACGAG 1 cut(s) 411
BssT1I CCWWGG 3 cut(s) 165, 729, 742
Bst2BI CACGAG 1 cut(s) 411
Bst2UI CCWGG 2 cut(s) 990, 1071
Bst4CI ACNGT 3 cut(s) 539, 610, 1032
Bst6I CTCTTC 1 cut(s) 431
BstDEI CTNAG 5 cut(s) 97, 677, 793, 1166, 1305
BstDSI CCRYGG 2 cut(s) 165, 729
BstF5I GGATG 3 cut(s) 296, 1121, 1260
BstHHI GCGC 1 cut(s) 488
BstKTI GATC 5 cut(s) 199, 648, 894, 968, 1137
BstMBI GATC 5 cut(s) 196, 645, 891, 965, 1134
BstMWI GCNNNNNNNGC 2 cut(s) 699, 794
BstNI CCWGG 2 cut(s) 990, 1071
BstNSI RCATGY 1 cut(s) 72
BstSCI CCNGG 2 cut(s) 988, 1069
BstSFI CTRYAG 1 cut(s) 1173
BstSLI GKGCMC 1 cut(s) 1005
BstV1I GCAGC 6 cut(s) 122, 302, 437, 554, 714, 916
BstV2I GAAGAC 2 cut(s) 630, 952
BsuRI GGCC 1 cut(s) 37
BtgI CCRYGG 2 cut(s) 165, 729
BtsCI GGATG 3 cut(s) 296, 1121, 1260
BtsI GCAGTG 3 cut(s) 258, 1125, 1170
BtsIMutI CAGTG 7 cut(s) 250, 258, 606, 855, 1125, 1170, 1179
CaiI CAGNNNCTG 2 cut(s) 470, 1181
CfoI GCGC 1 cut(s) 488
Cfr13I GGNCC 2 cut(s) 36, 746
CsiI ACCWGGT 1 cut(s) 988
Csp6I GTAC 2 cut(s) 844, 1119
CviAII CATG 6 cut(s) 45, 69, 166, 649, 730, 1006
CviQI GTAC 2 cut(s) 844, 1119
DdeI CTNAG 5 cut(s) 97, 677, 793, 1166, 1305
DpnI GATC 5 cut(s) 198, 647, 893, 967, 1136
DpnII GATC 5 cut(s) 196, 645, 891, 965, 1134
DrdI GACNNNNNNGTC 1 cut(s) 744
DseDI GACNNNNNNGTC 1 cut(s) 744
Eam1104I CTCTTC 1 cut(s) 431
EarI CTCTTC 1 cut(s) 431
EciI GGCGGA 1 cut(s) 1000
Eco130I CCWWGG 3 cut(s) 165, 729, 742
Eco47I GGWCC 1 cut(s) 746
EcoO109I RGGNCCY 1 cut(s) 36
EcoRII CCWGG 2 cut(s) 988, 1069
EcoT14I CCWWGG 3 cut(s) 165, 729, 742
ErhI CCWWGG 3 cut(s) 165, 729, 742
FaeI CATG 6 cut(s) 48, 72, 169, 652, 733, 1009
FaqI GGGAC 1 cut(s) 1303
FatI CATG 6 cut(s) 44, 68, 165, 648, 729, 1005
FauI CCCGC 1 cut(s) 155
FbaI TGATCA 1 cut(s) 196
FblI GTMKAC 2 cut(s) 662, 1191
Fnu4HI GCNGC 8 cut(s) 111, 256, 316, 426, 543, 653, 703, 930
FokI GGATG 3 cut(s) 303, 1108, 1267
Fsp4HI GCNGC 8 cut(s) 111, 256, 316, 426, 543, 653, 703, 930
FspBI CTAG 3 cut(s) 122, 131, 779
GlaI GCGC 1 cut(s) 487
GluI GCNGC 8 cut(s) 111, 256, 316, 426, 543, 653, 703, 930
GsaI CCCAGC 1 cut(s) 883
HaeIII GGCC 1 cut(s) 37
HhaI GCGC 1 cut(s) 488
Hin1II CATG 6 cut(s) 48, 72, 169, 652, 733, 1009
Hin6I GCGC 1 cut(s) 486
HinP1I GCGC 1 cut(s) 486
HincII GTYRAC 1 cut(s) 6
HindII GTYRAC 1 cut(s) 6
HindIII AAGCTT 1 cut(s) 86
HinfI GANTC 5 cut(s) 391, 528, 850, 1047, 1152
HphI GGTGA 3 cut(s) 33, 1064, 1288
Hpy166II GTNNAC 5 cut(s) 6, 557, 663, 749, 1192
Hpy188I TCNGA 5 cut(s) 192, 435, 454, 527, 997
Hpy188III TCNNGA 4 cut(s) 200, 278, 532, 1094
Hpy8I GTNNAC 5 cut(s) 6, 557, 663, 749, 1192
HpyAV CCTTC 1 cut(s) 36
HpyCH4III ACNGT 3 cut(s) 539, 610, 1032
HpyCH4IV ACGT 1 cut(s) 285
HpyCH4V TGCA 9 cut(s) 110, 161, 318, 323, 425, 542, 606, 1130, 1175
HpyF10VI GCNNNNNNNGC 2 cut(s) 699, 794
HpyF3I CTNAG 5 cut(s) 97, 677, 793, 1166, 1305
HpySE526I ACGT 1 cut(s) 285
Hsp92II CATG 6 cut(s) 48, 72, 169, 652, 733, 1009
HspAI GCGC 1 cut(s) 486
Ksp22I TGATCA 1 cut(s) 196
Kzo9I GATC 5 cut(s) 196, 645, 891, 965, 1134
LmnI GCTCC 3 cut(s) 82, 170, 491
Lsp1109I GCAGC 6 cut(s) 122, 302, 437, 554, 714, 916
LweI GCATC 1 cut(s) 678
MabI ACCWGGT 1 cut(s) 988
MaeI CTAG 3 cut(s) 122, 131, 779
MaeII ACGT 1 cut(s) 285
MaeIII GTNAC 3 cut(s) 896, 1177, 1286
MalI GATC 5 cut(s) 198, 647, 893, 967, 1136
MboI GATC 5 cut(s) 196, 645, 891, 965, 1134
MboII GAAGA 6 cut(s) 448, 454, 635, 664, 762, 957
MfeI CAATTG 2 cut(s) 51, 156
MhlI GDGCHC 1 cut(s) 1005
MlyI GAGTC 1 cut(s) 400
MmeI TCCRAC 1 cut(s) 1218
MseI TTAA 4 cut(s) 213, 642, 698, 869
MslI CAYNNNNRTG 1 cut(s) 1130
MspA1I CMGCKG 1 cut(s) 655
MspR9I CCNGG 2 cut(s) 990, 1071
MunI CAATTG 2 cut(s) 51, 156
MvaI CCWGG 2 cut(s) 990, 1071
MwoI GCNNNNNNNGC 2 cut(s) 699, 794
NcoI CCATGG 2 cut(s) 165, 729
NdeII GATC 5 cut(s) 196, 645, 891, 965, 1134
NlaIII CATG 6 cut(s) 48, 72, 169, 652, 733, 1009
NlaIV GGNNCC 3 cut(s) 145, 1002, 1029
NmuCI GTSAC 2 cut(s) 896, 1177
NspI RCATGY 1 cut(s) 72
PciI ACATGT 1 cut(s) 68
PfeI GAWTC 4 cut(s) 528, 850, 1047, 1152
PkrI GCNGC 8 cut(s) 112, 257, 317, 427, 544, 654, 704, 931
PleI GAGTC 1 cut(s) 399
PpsI GAGTC 1 cut(s) 399
PscI ACATGT 1 cut(s) 68
PsiI TTATAA 1 cut(s) 818
Psp6I CCWGG 2 cut(s) 988, 1069
PspFI CCCAGC 1 cut(s) 879
PspGI CCWGG 2 cut(s) 988, 1069
PspN4I GGNNCC 3 cut(s) 145, 1002, 1029
PspPI GGNCC 2 cut(s) 36, 746
PstI CTGCAG 1 cut(s) 1177
PstNI CAGNNNCTG 2 cut(s) 470, 1181
RsaI GTAC 2 cut(s) 845, 1120
RsaNI GTAC 2 cut(s) 844, 1119
RseI CAYNNNNRTG 1 cut(s) 1130
SaqAI TTAA 4 cut(s) 213, 642, 698, 869
SatI GCNGC 8 cut(s) 111, 256, 316, 426, 543, 653, 703, 930
Sau3AI GATC 5 cut(s) 196, 645, 891, 965, 1134
Sau96I GGNCC 2 cut(s) 36, 746
SchI GAGTC 1 cut(s) 400
ScrFI CCNGG 2 cut(s) 990, 1071
SduI GDGCHC 1 cut(s) 1005
SetI ASST 8 cut(s) 79, 90, 207, 288, 630, 744, 991, 1002
SexAI ACCWGGT 1 cut(s) 988
SfaNI GCATC 1 cut(s) 678
SfcI CTRYAG 1 cut(s) 1173
SinI GGWCC 1 cut(s) 746
SmiMI CAYNNNNRTG 1 cut(s) 1130
SmlI CTYRAG 2 cut(s) 31, 839
SmoI CTYRAG 2 cut(s) 31, 839
SpeI ACTAGT 1 cut(s) 121
SsiI CCGC 6 cut(s) 148, 255, 653, 826, 1011, 1234
SspMI CTAG 3 cut(s) 122, 131, 779
StyD4I CCNGG 2 cut(s) 988, 1069
StyI CCWWGG 3 cut(s) 165, 729, 742
TaaI ACNGT 3 cut(s) 539, 610, 1032
TaiI ACGT 1 cut(s) 288
TaqI TCGA 1 cut(s) 939
TaqII GACCGA 1 cut(s) 884
TatI WGTACW 2 cut(s) 843, 1118
TauI GCSGC 2 cut(s) 258, 655
TfiI GAWTC 4 cut(s) 528, 850, 1047, 1152
Tru1I TTAA 4 cut(s) 213, 642, 698, 869
Tru9I TTAA 4 cut(s) 213, 642, 698, 869
TscAI CASTG 7 cut(s) 250, 265, 613, 862, 1132, 1177, 1186
TseFI GTSAC 2 cut(s) 896, 1177
TseI GCWGC 6 cut(s) 110, 315, 425, 542, 702, 929
Tsp45I GTSAC 2 cut(s) 896, 1177
TspDTI ATGAA 4 cut(s) 762, 820, 995, 1052
TspRI CASTG 7 cut(s) 250, 265, 613, 862, 1132, 1177, 1186
VpaK11BI GGWCC 1 cut(s) 746
XapI RAATTY 3 cut(s) 309, 1097, 1279
XceI RCATGY 1 cut(s) 72
XmiI GTMKAC 2 cut(s) 662, 1191
XspI CTAG 3 cut(s) 122, 131, 779
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.