Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0000999

Physical Location & Seq

Genomic Coordinates Forward (+)

3459039 .. 3465974

Gene Structure 6936 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1049200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3126 bp

ATGACGGATACGACGGACGACATAGCGGAGGAAATCTCCTTCCAGAGCTTCGAAGACGACTGCAGAATGCTCGGCAGCCTTCTGCAGGAGGTGTTGCAGCGAGAGGTCCGAAGCCAAATCATGGAGAAAGTCGAAAGGATCCGAATCCTCGCTCAGAGTGCTTGTAATATGAGGAATGCGGGGATAGAGGACGTTGCGGAGGTGCTGGAGAAGCAACTGGCGTCGGAAATATCAAAGATGAGCTTGGAGGAGGCCTTGATCCTCGCTCGTACTTTCAGCCACTACCTTAATTTGATGGGCATTGCCGAAACCCATCACAGGGTTCGCAAGCAGCGAAATGACTCGAATGAGGCATCTCTATCGAAATCTTGTGACGATATATTTAATCAGCTTGTTCATGGTGGTGTTTCCCCAGACGAGCTTTATAATACTGTTTGCAAGCAGGAGGTTGAAATTGTTCTTACTGCACATCCTACACAAATTAATCGTCGCACCTTACAATACAAACATATAAGAATTGCTCATCTTTTAGACTACAAGCACCGACCTGATCTTGGCGATGAAGACAAAGAAATGCTGATTGAAGATCTGGTGAGAGAAATCACTTCAATATGGCAAACAGATGAGCTTAGGCGCCATAAACCTACGCCGGTTGATGAAGCTAGGGCAGGTTTGAACATTGTGGAGCAATCCCTTTGGAAAGCTGTACCTCATTATCTACGTCGAGTCAGCAATGCTTTGAAGAAGCATACTGGAAAACCACTTCCATTAACTTGCACACCTATAAAATTTGGGTCTTGGATGGGGGGTGACAGAGATGGAAATCCCAATGTAACAGCAAAGGTCACAAAAGATGTCTCACTTTTATCTAAGTGGATGGCTATTGATCTCTACATTCGAGAACTTGATAACCTCAAATTTGAGCTATCCATGAACCGTTGCAGTGATCGACTGTCAAGGCTGGCAGATGAAATTCTAGAGCAAGAAACTTCATCTGAGGATCGCCATGAGAATTGGCATCAGTACTTTGGTAGACATCAGTCGAAGCAACAAGGTGCGGCTCTTCCATCACAACTTCCAGCCAGAGCTGATCAACCCTCGTGCACAGACTGCATTCCTCGAATTGAAATTCCGAGGGCTAATTACCTGCTGAACCATCAGGACGGTCAGGACTCTCCAATGTCAAATCCTTCATCCCAGAATCCTTTTCGCAATGGGCATACAATACCAAATGGATCTTCAGGGTCTCCCCCTTCTAATTCAAGTCGACTTCTTAACCAAAGGAAAATGTTTGCAGAGTCCCAGATTGGAAGGTCTAGTTTCCAAAAGCTTTTAGAGCCAAGGCCTCCTCAGAGATCTGGAATTGCTCCGTATAGAGTGGTTCTTGGTAATGTAAAGGATAAGCTTATGAAGACACGAACACGTTTGGAGCTTCTTCTTGAAAATCTTCCTTGTGACTATGACCCATGGGATTGCTATGACACTACAGATCAACTTTTAGAACCGCTGCTCCTATGCTACGAGTCTTTGCAATCATGTGGATCTGGGGTGCTAGCTGACGGTCGGCTTGCTGATCTGATCCGAAGAGTTGCTACGTTTGGGATGGTACTAATGAAGCTCGACTTGCGTCAGGAATCTGGTAGGCATGCTGAAACACTTGATGCTGTTACCAAATATTTGGATATGGGTACATACAGTGAGTGGGATGAAGAAAAGAAACTCAAATTTCTTACTAAAGAGCTCAAAGGGAAGAGACCACTGGTTCCAAAAAGCATGGAGGTTGGTCCTGATGTTAAGGAAGTACTTGATACCTTCCACATTGCTGCTGAGCTGGGGAGTGATTCCCTTGGAGCCTATGTGATTTCTATGGCGTCAAGCGCAAGTGATGTTCTCGCGGTCGAGCTGTTGCAGAAAGATGCACGACTTGCTGTTAGTGGGGGGCTAGGAGGGCCGTGTCCTGGTGGAACGCTGCGGGTGGTTCCTTTGTTTGAAACTGTGAAGGACTTGAGAGAAGCTGGCTCGGCGATCAGAAAATTATTATCCATTGATTGGTACCGGGATCACATAATCAAGAACCATAATGGCCTTCAAGAGGTGATGGTTGGATATTCCGATTCTGGTAAAGATGCTGGGCGTTTTACTGCAGCATGGGAACTTTACAAAGCCCAAGAGGATGTTGTGGCCGCATGTAATGAATTCGGTATTAAGGTTACCCTGTTCCACGGACGAGGAGGAAGTATTGGTCGTGGAGGTGGCCCTACATATCTTGCCATTCAATCCCAACCGCCTGGTTCTGTAATGGGTACCCTACGGTCAACTGAGCAAGGAGAAATGGTTCAGGCAAAATTTGGGCTGCCACAAACTGCTGTCAGGCAGCTAGAAATATACACAACAGCTGTGCTGCTAGCAACCATACGCCCTCCACACTCACCTCGTGAAGAAAAGTGGCGTAACCTCATGGAGGAAATCTCAAAAATCAGTTGCCAGAACTACCGGAGCGTAGTCTATGAAAATCCAGAATTCCTTGCCTACTTCCATGAGGCTACACCTCAGGCTGAGCTTGGCTTCCTCAACATAGGAAGCCGCCCCACAAGAAGAAAGAGCTCCACAGGAATCGGACATCTTCGTGCAATTCCATGGGTGTTTGCATGGACCCAAACCAGATTTGTTCTTCCGTCATGGCTCGGAGTTGGAGCAGGTTTAAGGGGGGTTTGCGAGAAGGGACACACCGAAGACTTAAAAGCCATGTACAAAGAATGGCCTTTCTTTCAATGTACCCTTGACCTTATAGAGATGATTTTGGGGAAGGCAGACGCTCCTATAGCCAAACACTACGACGAAGTCCTTGTCTCAGAAAGCAGGCGACATCTTGGTTCCGAGCTGCGAAAGGAGCTCTTGACTACAGAGAAGTATGTATTGGTGGTTAGTGGGCACGAGAAACTATCTGAGAATAATCGGAGCTTGAGGAAGCTGATTGAAAGCAGGCTCCCCTTTCTCAATCCTATGAACATGTTGCAGGTTGAGGTACTCAAGAGACTGAGATGCGATGACGATAACAATAAACTCCGGGATGCATTGCTCATCACAATAAATGGGATCGCCGCAGGGATGAGAAACACAGGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004611 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006082 GO:0006091 GO:0006099 GO:0006101 GO:0008150 GO:0008152 GO:0008964 GO:0009060 GO:0009987 GO:0015980 GO:0016829 GO:0016830 GO:0016831 GO:0016999 GO:0017144 GO:0019752 GO:0043436 GO:0044237 GO:0044238 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0045333 GO:0055114 GO:0071704 GO:0072350

KEGG Pathways

Metabolic & Signaling

ko00620 ko00680 ko00710 ko00720 ko01100 ko01120 ko01200 map00620 map00680 map00710 map00720 map01100 map01120 map01200

Pfam Domains

Protein Families

PEPcase

Protein Analysis

1042

Amino Acids

117.26

Weight (kDa)

6.51

Isoelectric Point (pI)

52.76

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PEPcase	PF00311	18 - 333	3.7e-85	Phosphoenolpyruvate carboxylase
PEPcase	PF00311	454 - 1041	1.1e-228	Phosphoenolpyruvate carboxylase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000999)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G68750
fragaria_vesca	FvH4_3g20961 FvH4_4g28350 FvH4_4g28350 FvH4_4g28350 FvH4_4g28350 FvH4_4g28350 FvH4_4g28350 FvH4_4g28350 FvH4_4g28350 FvH4_4g28350 FvH4_4g28350 FvH4_4g28350
malus_domestica	MD13G1049200.v1.1 MD16G1050300.v1.1
prunus_persica	Prupe.1G302700_v2.0.a1 Prupe.1G302700_v2.0.a1
pyrus_communis	pycom16g04410
rosa_chinensis	RchiOBHm_Chr1g0330331 RchiOBHm_Chr1g0355581 RchiOBHm_Chr1g0379911 RchiOBHm_Chr4g0407141 RchiOBHm_Chr4g0436201 RchiOBHm_Chr5g0035501 RchiOBHm_Chr5g0035601 RchiOBHm_Chr5g0035681 RchiOBHm_Chr5g0035861 RchiOBHm_Chr5g0063691 RchiOBHm_Chr6g0312331
rosa_laevigata	RLG00000006529 RLG00000033624 RLG00000033642 RLG00000033654
rosa_multiflora	Rmu_sc0000084.1_g000022 Rmu_sc0006173.1_g000008 Rmu_sc0007205.1_g000021 Rmu_sc0014259.1_g000014
rosa_roxburghii	Rroxscaffold_1G00021870 Rroxscaffold_1G00044830 Rroxscaffold_1G00044890 Rroxscaffold_1G00044970 Rroxscaffold_3G00231670 Rroxscaffold_5G00377400 Rroxscaffold_7G00185100
rosa_rugosa	Rorug03G0294000 Rorug04G0292500 Rorug04G0292600 Rorug04G0292600
rosa_samantha	Rh4AG346800 Rh4BG355700 Rh4CG370400 Rh4DG349400 Rh5AG245000 Rh5BG244800 Rh5CG276300 Rh5CG276800 Rh5DG253900 Rh5DG464900 Rh6BG126500
rosa_wichuraiana	Rw1G008440 Rw4G030310 Rw5G022500

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	426
Acc36I	ACCTGC	4 cut(s)	659, 1155, 2687, 3007
Acc65I	GGTACC	2 cut(s)	2052, 2303
AccB1I	GGYRCC	3 cut(s)	633, 2052, 2303
AccB7I	CCANNNNNTGG	2 cut(s)	121, 2049
AccI	GTMKAC	2 cut(s)	1033, 1267
AccII	CGCG	1 cut(s)	1895
AclWI	GGATC	9 cut(s)	133, 146, 253, 1008, 1243, 1549, 1573, 2067, 3104
AcoI	YGGCCR	1 cut(s)	2181
AcsI	RAATTY	8 cut(s)	788, 917, 972, 1128, 1724, 2195, 2345, 2519
AcuI	CTGAAG	1 cut(s)	1224
AcyI	GRCGYC	3 cut(s)	221, 634, 1871
AdeI	CACNNNGTG	1 cut(s)	2435
AfiI	CCNNNNNNNGG	9 cut(s)	85, 121, 318, 319, 554, 1958, 2049, 2092, 2461
AflIII	ACRYGT	2 cut(s)	1421, 3009
AjnI	CCWGG	2 cut(s)	1957, 2287
AjuI	GAANNNNNNNTTGG	4 cut(s)	1222, 1254, 2900, 2932
AloI	GAACNNNNNNTCC	4 cut(s)	1494, 1526, 2319, 2351
Alw21I	GWGCWC	4 cut(s)	1106, 1743, 2606, 2895
Alw26I	GTCTC	5 cut(s)	862, 1251, 1747, 2854, 3028
Alw44I	GTGCAC	1 cut(s)	1102
AlwI	GGATC	9 cut(s)	133, 146, 253, 1008, 1243, 1549, 1573, 2067, 3104
AoxI	GGCC	7 cut(s)	252, 1343, 1949, 2083, 2181, 2254, 2759
ApaLI	GTGCAC	1 cut(s)	1102
ApoI	RAATTY	8 cut(s)	788, 917, 972, 1128, 1724, 2195, 2345, 2519
ArsI	GACNNNNNNTTYG	4 cut(s)	472, 504, 2832, 2864
AseI	ATTAAT	1 cut(s)	483
Asp700I	GAANNNNTTC	3 cut(s)	456, 1446, 2334
Asp718I	GGTACC	2 cut(s)	2052, 2303
AspLEI	GCGC	2 cut(s)	636, 1880
AspS9I	GGNCC	5 cut(s)	106, 1784, 1949, 2255, 2652
AsuC2I	CCSGG	2 cut(s)	2057, 3068
AsuHPI	GGTGA	4 cut(s)	604, 821, 2107, 2421
AsuII	TTCGAA	1 cut(s)	51
AsuNHI	GCTAGC	2 cut(s)	1552, 2404
AvaII	GGWCC	3 cut(s)	106, 1784, 2652
AxyI	CCTNAGG	1 cut(s)	2550
BaeGI	GKGCMC	2 cut(s)	1106, 2934
BamHI	GGATCC	1 cut(s)	138
BanI	GGYRCC	3 cut(s)	633, 2052, 2303
BanII	GRGCYC	3 cut(s)	1743, 2606, 2895
BarI	GAAGNNNNNNTAC	2 cut(s)	1576, 1608
BauI	CACGAG	3 cut(s)	1099, 2433, 2933
BbsI	GAAGAC	4 cut(s)	60, 570, 1418, 2739
Bbv12I	GWGCWC	4 cut(s)	1106, 1743, 2606, 2895
BccI	CCATC	9 cut(s)	289, 321, 796, 812, 871, 1075, 1164, 1597, 2092
BceAI	ACGGC	1 cut(s)	1936
BcgI	CGANNNNNNTGC	4 cut(s)	52, 86, 342, 376
BciT130I	CCWGG	2 cut(s)	1959, 2289
BclI	TGATCA	1 cut(s)	1090
BcnI	CCSGG	2 cut(s)	2057, 3068
BcoDI	GTCTC	5 cut(s)	862, 1251, 1747, 2854, 3028
BfaI	CTAG	7 cut(s)	663, 977, 1317, 1553, 1943, 2378, 2405
BfmI	CTRYAG	6 cut(s)	61, 83, 1485, 2142, 2820, 2901
BfoI	RGCGCY	1 cut(s)	637
BfuAI	ACCTGC	4 cut(s)	659, 1155, 2687, 3007
BglII	AGATCT	2 cut(s)	586, 1355
BlpI	GCTNAGC	2 cut(s)	1827, 2556
BmcAI	AGTACT	2 cut(s)	1025, 1803
Bme1390I	CCNGG	4 cut(s)	1959, 2057, 2289, 3068
Bme18I	GGWCC	3 cut(s)	106, 1784, 2652
BmgT120I	GGNCC	5 cut(s)	106, 1784, 1949, 2255, 2652
BmrFI	CCNGG	4 cut(s)	1959, 2057, 2289, 3068
BmsI	GCATC	7 cut(s)	362, 1027, 1651, 1906, 2116, 3032, 3061
BmtI	GCTAGC	2 cut(s)	1556, 2408
BoxI	GACNNNNGTC	2 cut(s)	1039, 1626
BpiI	GAAGAC	4 cut(s)	60, 570, 1418, 2739
BplI	GAGNNNNNCTC	4 cut(s)	20, 52, 2453, 2485
BpmI	CTGGAG	1 cut(s)	227
Bpu10I	CCTNAGC	1 cut(s)	629
Bpu1102I	GCTNAGC	2 cut(s)	1827, 2556
Bpu14I	TTCGAA	1 cut(s)	51
BpuEI	CTTGAG	3 cut(s)	2026, 2983, 3014
BpuMI	CCSGG	2 cut(s)	2057, 3068
BsaBI	GATNNNNATC	2 cut(s)	143, 822
BsaHI	GRCGYC	3 cut(s)	221, 634, 1871
BsaI	GGTCTC	2 cut(s)	1251, 1747
BsaJI	CCNNGG	6 cut(s)	1133, 1340, 1466, 1846, 2221, 2636
BsaWI	WCCGGW	1 cut(s)	2493
BsaXI	ACNNNNNCTCC	6 cut(s)	1494, 1524, 1842, 1872, 2319, 2349
Bsc4I	CCNNNNNNNGG	9 cut(s)	85, 121, 318, 319, 554, 1958, 2049, 2092, 2461
Bse118I	RCCGGY	1 cut(s)	649
Bse1I	ACTGG	3 cut(s)	222, 757, 1764
Bse21I	CCTNAGG	1 cut(s)	2550
Bse3DI	GCAATG	5 cut(s)	300, 739, 1219, 1818, 3074
Bse8I	GATNNNNATC	2 cut(s)	143, 822
BseBI	CCWGG	2 cut(s)	1959, 2289
BseDI	CCNNGG	6 cut(s)	1133, 1340, 1466, 1846, 2221, 2636
BseGI	GGATG	9 cut(s)	469, 807, 882, 1193, 1608, 1711, 2179, 3076, 3114
BseJI	GATNNNNATC	2 cut(s)	143, 822
BseLI	CCNNNNNNNGG	9 cut(s)	85, 121, 318, 319, 554, 1958, 2049, 2092, 2461
BseMI	GCAATG	5 cut(s)	300, 739, 1219, 1818, 3074
BseNI	ACTGG	3 cut(s)	222, 757, 1764
BseRI	GAGGAG	3 cut(s)	263, 1338, 2244
BseSI	GKGCMC	2 cut(s)	1106, 2934
BseYI	CCCAGC	2 cut(s)	1831, 2129
BsgI	GTGCAG	1 cut(s)	450
Bsh1236I	CGCG	1 cut(s)	1895
Bsh1285I	CGRYCG	2 cut(s)	1564, 1899
BshFI	GGCC	7 cut(s)	254, 1345, 1951, 2085, 2183, 2256, 2761
BshNI	GGYRCC	3 cut(s)	633, 2052, 2303
BsiEI	CGRYCG	2 cut(s)	1564, 1899
BsiHKAI	GWGCWC	4 cut(s)	1106, 1743, 2606, 2895
BsiSI	CCGG	4 cut(s)	650, 2056, 2494, 3067
BslFI	GGGAC	2 cut(s)	1285, 2736
BslI	CCNNNNNNNGG	9 cut(s)	85, 121, 318, 319, 554, 1958, 2049, 2092, 2461
BsmAI	GTCTC	5 cut(s)	862, 1251, 1747, 2854, 3028
BsmFI	GGGAC	2 cut(s)	1285, 2736
BsmI	GAATGC	3 cut(s)	72, 181, 1113
BsnI	GGCC	7 cut(s)	254, 1345, 1951, 2085, 2183, 2256, 2761
Bso31I	GGTCTC	2 cut(s)	1251, 1747
Bsp119I	TTCGAA	1 cut(s)	51
Bsp1286I	GDGCHC	5 cut(s)	1106, 1743, 2606, 2895, 2934
Bsp1407I	TGTACA	1 cut(s)	2748
Bsp1720I	GCTNAGC	2 cut(s)	1827, 2556
Bsp19I	CCATGG	2 cut(s)	1466, 2636
BspANI	GGCC	7 cut(s)	254, 1345, 1951, 2085, 2183, 2256, 2761
BspFNI	CGCG	1 cut(s)	1895
BspMAI	CTGCAG	3 cut(s)	65, 87, 2146
BspMI	ACCTGC	4 cut(s)	659, 1155, 2687, 3007
BspOI	GCTAGC	2 cut(s)	1556, 2408
BspPI	GGATC	9 cut(s)	133, 146, 253, 1008, 1243, 1549, 1573, 2067, 3104
BspQI	GCTCTTC	1 cut(s)	1068
BspT104I	TTCGAA	1 cut(s)	51
BspT107I	GGYRCC	3 cut(s)	633, 2052, 2303
BspTNI	GGTCTC	2 cut(s)	1251, 1747
BsrDI	GCAATG	5 cut(s)	300, 739, 1219, 1818, 3074
BsrFI	RCCGGY	1 cut(s)	649
BsrGI	TGTACA	1 cut(s)	2748
BsrI	ACTGG	3 cut(s)	222, 757, 1764
BssAI	RCCGGY	1 cut(s)	649
BssECI	CCNNGG	6 cut(s)	1133, 1340, 1466, 1846, 2221, 2636
BssNI	GRCGYC	3 cut(s)	221, 634, 1871
BssSI	CACGAG	3 cut(s)	1099, 2433, 2933
BssT1I	CCWWGG	4 cut(s)	1340, 1466, 1846, 2636
Bst2BI	CACGAG	3 cut(s)	1099, 2433, 2933
Bst2UI	CCWGG	2 cut(s)	1959, 2289
Bst4CI	ACNGT	8 cut(s)	433, 938, 954, 1166, 1562, 1697, 1996, 2313
Bst6I	CTCTTC	3 cut(s)	1068, 1579, 1745
BstACI	GRCGYC	3 cut(s)	221, 634, 1871
BstAPI	GCANNNNNTGC	2 cut(s)	1110, 1925
BstAUI	TGTACA	1 cut(s)	2748
BstBI	TTCGAA	1 cut(s)	51
BstDSI	CCRYGG	3 cut(s)	1466, 2221, 2636
BstEII	GGTNACC	1 cut(s)	2209
BstENI	CCTNNNNNAGG	3 cut(s)	83, 2090, 2459
BstF5I	GGATG	9 cut(s)	469, 807, 882, 1193, 1608, 1711, 2179, 3076, 3114
BstFNI	CGCG	1 cut(s)	1895
BstH2I	RGCGCY	1 cut(s)	637
BstHHI	GCGC	2 cut(s)	636, 1880
BstMAI	GTCTC	5 cut(s)	862, 1251, 1747, 2854, 3028
BstMCI	CGRYCG	2 cut(s)	1564, 1899
BstNI	CCWGG	2 cut(s)	1959, 2289
BstNSI	RCATGY	3 cut(s)	1649, 2190, 3013
BstPAI	GACNNNNGTC	2 cut(s)	1039, 1626
BstPI	GGTNACC	1 cut(s)	2209
BstSCI	CCNGG	4 cut(s)	1957, 2055, 2287, 3066
BstSFI	CTRYAG	6 cut(s)	61, 83, 1485, 2142, 2820, 2901
BstSLI	GKGCMC	2 cut(s)	1106, 2934
BstUI	CGCG	1 cut(s)	1895
BstV2I	GAAGAC	4 cut(s)	60, 570, 1418, 2739
BstX2I	RGATCY	5 cut(s)	138, 586, 1235, 1355, 1541
BstXI	CCANNNNNNTGG	2 cut(s)	1678, 2288
BstYI	RGATCY	5 cut(s)	138, 586, 1235, 1355, 1541
Bsu36I	CCTNAGG	1 cut(s)	2550
BsuRI	GGCC	7 cut(s)	254, 1345, 1951, 2085, 2183, 2256, 2761
BtgI	CCRYGG	3 cut(s)	1466, 2221, 2636
BtgZI	GCGATG	2 cut(s)	573, 3060
BtsCI	GGATG	9 cut(s)	469, 807, 882, 1193, 1608, 1711, 2179, 3076, 3114
BtsI	GCAGTG	1 cut(s)	949
BtsIMutI	CAGTG	3 cut(s)	949, 1702, 1757
BveI	ACCTGC	4 cut(s)	659, 1155, 2687, 3007
CfoI	GCGC	2 cut(s)	636, 1880
Cfr10I	RCCGGY	1 cut(s)	649
Cfr13I	GGNCC	5 cut(s)	106, 1784, 1949, 2255, 2652
CseI	GACGC	4 cut(s)	210, 1616, 1860, 2822
DinI	GGCGCC	1 cut(s)	635
DraIII	CACNNNGTG	1 cut(s)	2435
EaeI	YGGCCR	1 cut(s)	2181
Eam1104I	CTCTTC	3 cut(s)	1068, 1579, 1745
EarI	CTCTTC	3 cut(s)	1068, 1579, 1745
Ecl136II	GAGCTC	3 cut(s)	1741, 2604, 2893
Eco130I	CCWWGG	4 cut(s)	1340, 1466, 1846, 2636
Eco147I	AGGCCT	2 cut(s)	254, 1345
Eco24I	GRGCYC	3 cut(s)	1743, 2606, 2895
Eco31I	GGTCTC	2 cut(s)	1251, 1747
Eco47I	GGWCC	3 cut(s)	106, 1784, 2652
Eco53kI	GAGCTC	3 cut(s)	1741, 2604, 2893
Eco57I	CTGAAG	1 cut(s)	1224
Eco81I	CCTNAGG	1 cut(s)	2550
Eco91I	GGTNACC	1 cut(s)	2209
EcoICRI	GAGCTC	3 cut(s)	1741, 2604, 2893
EcoNI	CCTNNNNNAGG	3 cut(s)	83, 2090, 2459
EcoO65I	GGTNACC	1 cut(s)	2209
EcoRI	GAATTC	2 cut(s)	2195, 2519
EcoRII	CCWGG	2 cut(s)	1957, 2287
EcoT14I	CCWWGG	4 cut(s)	1340, 1466, 1846, 2636
EcoT22I	ATGCAT	1 cut(s)	3076
EcoT38I	GRGCYC	3 cut(s)	1743, 2606, 2895
EgeI	GGCGCC	1 cut(s)	635
EheI	GGCGCC	1 cut(s)	635
ErhI	CCWWGG	4 cut(s)	1340, 1466, 1846, 2636
FalI	AAGNNNNNCTT	6 cut(s)	227, 259, 1607, 1639, 2879, 2911
FaqI	GGGAC	2 cut(s)	1285, 2736
FauI	CCCGC	2 cut(s)	172, 1965
FbaI	TGATCA	1 cut(s)	1090
FblI	GTMKAC	2 cut(s)	1033, 1267
FokI	GGATG	9 cut(s)	456, 814, 889, 1180, 1615, 1718, 2186, 3083, 3121
FriOI	GRGCYC	3 cut(s)	1743, 2606, 2895
FspBI	CTAG	7 cut(s)	663, 977, 1317, 1553, 1943, 2378, 2405
GlaI	GCGC	2 cut(s)	635, 1879
GsaI	CCCAGC	2 cut(s)	1835, 2133
GsuI	CTGGAG	1 cut(s)	227
HaeII	RGCGCY	1 cut(s)	637
HaeIII	GGCC	7 cut(s)	254, 1345, 1951, 2085, 2183, 2256, 2761
HapII	CCGG	4 cut(s)	650, 2056, 2494, 3067
HgaI	GACGC	4 cut(s)	210, 1616, 1860, 2822
HhaI	GCGC	2 cut(s)	636, 1880
Hin1I	GRCGYC	3 cut(s)	221, 634, 1871
Hin6I	GCGC	2 cut(s)	634, 1878
HinP1I	GCGC	2 cut(s)	634, 1878
HincII	GTYRAC	2 cut(s)	1268, 2316
HindII	GTYRAC	2 cut(s)	1268, 2316
HindIII	AAGCTT	2 cut(s)	1328, 1403
HpaII	CCGG	4 cut(s)	650, 2056, 2494, 3067
HphI	GGTGA	4 cut(s)	604, 821, 2107, 2421
Hpy166II	GTNNAC	4 cut(s)	1034, 1104, 1268, 2316
Hpy8I	GTNNAC	4 cut(s)	1034, 1104, 1268, 2316
Hpy99I	CGWCG	5 cut(s)	16, 226, 492, 726, 2840
HpyCH4III	ACNGT	8 cut(s)	433, 938, 954, 1166, 1562, 1697, 1996, 2313
HpyCH4IV	ACGT	4 cut(s)	192, 721, 1423, 1595
HpySE526I	ACGT	4 cut(s)	192, 721, 1423, 1595
Hsp92I	GRCGYC	3 cut(s)	221, 634, 1871
HspAI	GCGC	2 cut(s)	634, 1878
KasI	GGCGCC	1 cut(s)	633
KpnI	GGTACC	2 cut(s)	2056, 2307
Ksp22I	TGATCA	1 cut(s)	1090
LguI	GCTCTTC	1 cut(s)	1068
LweI	GCATC	7 cut(s)	362, 1027, 1651, 1906, 2116, 3032, 3061
MaeI	CTAG	7 cut(s)	663, 977, 1317, 1553, 1943, 2378, 2405
MaeII	ACGT	4 cut(s)	192, 721, 1423, 1595
MaeIII	GTNAC	8 cut(s)	371, 809, 832, 844, 1454, 1666, 2209, 2450
MflI	RGATCY	5 cut(s)	138, 586, 1235, 1355, 1541
MhlI	GDGCHC	5 cut(s)	1106, 1743, 2606, 2895, 2934
Mly113I	GGCGCC	1 cut(s)	634
MlyI	GAGTC	5 cut(s)	335, 735, 1166, 1307, 1532
MmeI	TCCRAC	3 cut(s)	204, 2083, 2671
Mph1103I	ATGCAT	1 cut(s)	3076
MroXI	GAANNNNTTC	3 cut(s)	456, 1446, 2334
MseI	TTAA	9 cut(s)	288, 384, 483, 770, 1275, 1794, 2205, 2702, 2738
MslI	CAYNNNNRTG	2 cut(s)	402, 2625
MspA1I	CMGCKG	2 cut(s)	1507, 2396
MspI	CCGG	4 cut(s)	650, 2056, 2494, 3067
MspR9I	CCNGG	4 cut(s)	1959, 2057, 2289, 3068
Mva1269I	GAATGC	3 cut(s)	72, 181, 1113
MvaI	CCWGG	2 cut(s)	1959, 2289
MvnI	CGCG	1 cut(s)	1895
NarI	GGCGCC	1 cut(s)	634
NciI	CCSGG	2 cut(s)	2057, 3068
NcoI	CCATGG	2 cut(s)	1466, 2636
NheI	GCTAGC	2 cut(s)	1552, 2404
NmeAIII	GCCGAG	2 cut(s)	51, 2000
NmuCI	GTSAC	4 cut(s)	371, 809, 844, 1454
NsiI	ATGCAT	1 cut(s)	3076
NspI	RCATGY	3 cut(s)	1649, 2190, 3013
NspV	TTCGAA	1 cut(s)	51
PaeI	GCATGC	1 cut(s)	1649
PceI	AGGCCT	2 cut(s)	254, 1345
PciI	ACATGT	1 cut(s)	3009
PciSI	GCTCTTC	1 cut(s)	1068
PcsI	WCGNNNNNNNCGW	1 cut(s)	331
PctI	GAATGC	3 cut(s)	72, 181, 1113
PdmI	GAANNNNTTC	3 cut(s)	456, 1446, 2334
PfeI	GAWTC	6 cut(s)	144, 1201, 1634, 1841, 2114, 2613
PflFI	GACNNNGTC	1 cut(s)	2840
PflMI	CCANNNNNTGG	2 cut(s)	121, 2049
PfoI	TCCNGGA	1 cut(s)	3066
PleI	GAGTC	5 cut(s)	335, 734, 1166, 1306, 1531
PluTI	GGCGCC	1 cut(s)	637
PpsI	GAGTC	5 cut(s)	335, 734, 1166, 1306, 1531
PscI	ACATGT	1 cut(s)	3009
PshAI	GACNNNNGTC	2 cut(s)	1039, 1626
PshBI	ATTAAT	1 cut(s)	483
PsiI	TTATAA	1 cut(s)	426
Psp124BI	GAGCTC	3 cut(s)	1743, 2606, 2895
Psp6I	CCWGG	2 cut(s)	1957, 2287
PspEI	GGTNACC	1 cut(s)	2209
PspFI	CCCAGC	2 cut(s)	1831, 2129
PspGI	CCWGG	2 cut(s)	1957, 2287
PspPI	GGNCC	5 cut(s)	106, 1784, 1949, 2255, 2652
PstI	CTGCAG	3 cut(s)	65, 87, 2146
PsuI	RGATCY	5 cut(s)	138, 586, 1235, 1355, 1541
PsyI	GACNNNGTC	1 cut(s)	2840
PvuII	CAGCTG	1 cut(s)	2396
RseI	CAYNNNNRTG	2 cut(s)	402, 2625
SacI	GAGCTC	3 cut(s)	1743, 2606, 2895
SalI	GTCGAC	1 cut(s)	1266
SapI	GCTCTTC	1 cut(s)	1068
SaqAI	TTAA	9 cut(s)	288, 384, 483, 770, 1275, 1794, 2205, 2702, 2738
Sau96I	GGNCC	5 cut(s)	106, 1784, 1949, 2255, 2652
ScaI	AGTACT	2 cut(s)	1025, 1803
SchI	GAGTC	5 cut(s)	335, 735, 1166, 1307, 1532
ScrFI	CCNGG	4 cut(s)	1959, 2057, 2289, 3068
SduI	GDGCHC	5 cut(s)	1106, 1743, 2606, 2895, 2934
SfaNI	GCATC	7 cut(s)	362, 1027, 1651, 1906, 2116, 3032, 3061
SfcI	CTRYAG	6 cut(s)	61, 83, 1485, 2142, 2820, 2901
SfoI	GGCGCC	1 cut(s)	635
SfuI	TTCGAA	1 cut(s)	51
SinI	GGWCC	3 cut(s)	106, 1784, 2652
SmiMI	CAYNNNNRTG	2 cut(s)	402, 2625
SmlI	CTYRAG	3 cut(s)	2005, 2962, 3029
SmoI	CTYRAG	3 cut(s)	2005, 2962, 3029
SphI	GCATGC	1 cut(s)	1649
SseBI	AGGCCT	2 cut(s)	254, 1345
SspDI	GGCGCC	1 cut(s)	633
SspI	AATATT	1 cut(s)	1676
SspMI	CTAG	7 cut(s)	663, 977, 1317, 1553, 1943, 2378, 2405
SstI	GAGCTC	3 cut(s)	1743, 2606, 2895
StuI	AGGCCT	2 cut(s)	254, 1345
StyD4I	CCNGG	4 cut(s)	1957, 2055, 2287, 3066
StyI	CCWWGG	4 cut(s)	1340, 1466, 1846, 2636
TaaI	ACNGT	8 cut(s)	433, 938, 954, 1166, 1562, 1697, 1996, 2313
TaiI	ACGT	4 cut(s)	195, 724, 1426, 1598
TatI	WGTACW	3 cut(s)	1023, 1801, 2748
TauI	GCSGC	4 cut(s)	1061, 2186, 2586, 3104
TfiI	GAWTC	6 cut(s)	144, 1201, 1634, 1841, 2114, 2613
Tru1I	TTAA	9 cut(s)	288, 384, 483, 770, 1275, 1794, 2205, 2702, 2738
Tru9I	TTAA	9 cut(s)	288, 384, 483, 770, 1275, 1794, 2205, 2702, 2738
TscAI	CASTG	3 cut(s)	949, 1702, 1764
TseFI	GTSAC	4 cut(s)	371, 809, 844, 1454
Tsp45I	GTSAC	4 cut(s)	371, 809, 844, 1454
TspGWI	ACGGA	5 cut(s)	20, 29, 1359, 2238, 2664
TspRI	CASTG	3 cut(s)	949, 1702, 1764
Tth111I	GACNNNGTC	1 cut(s)	2840
Van91I	CCANNNNNTGG	2 cut(s)	121, 2049
VneI	GTGCAC	1 cut(s)	1102
VpaK11BI	GGWCC	3 cut(s)	106, 1784, 2652
VspI	ATTAAT	1 cut(s)	483
XagI	CCTNNNNNAGG	3 cut(s)	83, 2090, 2459
XapI	RAATTY	8 cut(s)	788, 917, 972, 1128, 1724, 2195, 2345, 2519
XbaI	TCTAGA	1 cut(s)	976
XceI	RCATGY	3 cut(s)	1649, 2190, 3013
XmiI	GTMKAC	2 cut(s)	1033, 1267
XmnI	GAANNNNTTC	3 cut(s)	456, 1446, 2334
XspI	CTAG	7 cut(s)	663, 977, 1317, 1553, 1943, 2378, 2405
ZrmI	AGTACT	2 cut(s)	1025, 1803
Zsp2I	ATGCAT	1 cut(s)	3076

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.