Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4A

Orthogroup ID

OG0000999

Physical Location & Seq

Genomic Coordinates Forward (+)

65514076 .. 65522969

Gene Structure 8894 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4AG346800.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3093 bp

ATGACGGACACGACGGACGATATCGCCGAGGAAATCTCGTTCCAGAGCTTCGAAGACGATTGTAAAATGCTCGGCAATCTTCTCCTGGAGGTTTTGCAGCGAGAAGTCGGAGCTCAGATGAGGGAGAAAGTCGACCGCTACAGAACCCTCGCTCAGAGTGCTTGTAGTATGAGGCTAGCGGGGATAGAGCACATGGCGGAGCTGCTGGAGAAGCAGCTAGCGTCCGACATTTCGAATATGAGCTTAGAGGAGGCACTCACTCTCGCTCGTGCTTTCGGCCATTACCTCAATTTGATGGGCATTGCTGAAGTTCATCATAGGGTTCGTAGACAAAAGAATGTGGCATCCCTATCAAAATCTTGTGATGATATTTTTTATCAGCTTCTGCAGCGTGGAGTTTCCCCGGACCAGCTTTACGAGACTGTTTGCAAGCAGGAGGTTGAGATTGTTCTTACTGCACATCCTACACAAATTAATCGCCGAACCTTACAATACAAACATATCAAACTTTCTCATCTTTTAGATTTAAAAGACCGACCTGATCTTACTACTGAAGAGAGAGATATGGTGATTGAAGATATGGTGCGAGAGATTACTTCTATATGGCAGACAGATGAGCTTAGGCGCCATAAACCCACACCAGTTGATGAAGCTAGTGCAGGTTTGAACATTGTGGAGCAGTCCCTTTGGAAAGCTGTACCTCATTATTTACGTCGTGTCAGCAATGCTTTAAAGAAGCACACTGGAAAATCACTTCCACTGAAGTGCACACCAATCAGGTTTGGGTCTTGGATGGGGGGTGATAGAGATGGAAATCCCAATGTGACAGCAAAGGTCACAAAAGATGTCTCACTTTTATCTAGGTGGATGACTATTGATCTCTATATTCGAGAAGTGGATAGCCTGAAATTTGAACTATCCATGAATAGGTGCAGCAATAGCTTGTCAAAACTGGCACATGAGATCCTAGAGCAAGAATCTGCATCTGAGGATCACCATAGTTGGGATCATCAGTGTTATAGTAGAAATCAGGTAAAGCAACAAGCTGCGGCACTTCCAACACAACTTCCAGCTAGAGCTGATCAACCCTCTTGCACTGAATGCAATGTTGGTCATTCTCACTATCCCAGAATTGAAATTCCTCGAACTGGGCTACTCAATCAGCAGGATGGTCCGGACTCTCCAAGATCTCCAATGGCAAGGTCTCCATCCCAGAACTCTCTTCGTAATGCTAGTCTCATTGCCAAGAGGAAAATGTTTGCTGAGTCCCAGGTTGGAAGGTCCAGTTTCCAGAAGCTTTTGGAGCCAAAGCCCACTAAGAGATCTGCAATTGCTCCTTATAGAATTGTTCTTGGTAATATAAAAGACAAGCTTATGAAGACACGAAGACGATTGGAGCTTCTTCTAGAGGATCTGCCTTGCGATTTTAATCTATCGGAGTATTATGACACATCAGATGAACTTATAGAACCACTGATGACATGCCATGACTCTTTGCAATCATGTGGATCTGGGATCCTAGCTGATGGTCGGCTTGCTGATTTGATCCGAAGAGTTGCTACCTTTGGGATGGTATTAATGAAGCTCGACTTGCGTCAGGAATCTGGTAGACATGTTGAAACATTTGATGCAATTACCAAATATTTGGATATGGGCACATACAGTGAGTGGGATGAAGAAAAGAAACTGGAATTTCTAACAAGAGAGCTCAAAGGAAAAAGACCGCTAGTCTCACCTAGCATGGAGGTTGCTTCTGATGTTAAAGAAGTACTGGATACCTTCCGCATTGCTGCTGAGCTAGGGAGTGATTCGCTTGGAGCATATGTGATTTCTATGGCTTCTAACGCAAGTGATGTCCTTGCTGTGGAGCTTTTGCAAAAAGATGCACGACTTGCCGTTAGTGGAGAAATAGGAAAGCCATGTCCTGGTGGAACGCTGCGTGTGGTTCCTTTGTTTGAAACTGTGAAGGACTTGAGAGGAGCTGGCTCAGTGATCAGGAAATTATTGTCCATTGATTGGTACAGGGAGCACATCATCAAGAACCATAATGGGCATCAAGAGGTGATGGTTGGATACTCTGATTCTGGAAAAGATGCAGGGCGTTTTACTGCTGCATGGGAACTTTACAAAGCTCAAGAGGATGTTGTAGCTGCATGCAATGAGTATGATATCAAAGTTACCCTGTTTCATGGGAGAGGAGGAAGTATTGGTCGTGGAGGGGGCCCTACATATCTGGCCATTCAATCCCAGCCGCCTGGCTCTGTGATGGGTACTCTACGATCAACTGAGCAAGGAGAAATGGTACAGGCAAAATTTGGGCTGCCACAGATTGCTGTCAGACAGCTAGAGATATACACAACAGCTGTGCTGCTTGCAACGTTGCGTCCTCCACACCCTCCTCGAGAACAAAAGTGGCGTAACCTCATGGAGGAGATCTCAAAAATCAGTTGTCAGAATTATAGGAGCGTAGTCTACGAAAATCCAGAATTCCTTACTTACTTCCAGGAGGCTACACCCCAGGCTGAGCTTGGCTTCCTCAACATAGGAAGCCGCCCCACAAGAAGAAAGAGCTCTACAGGAATTGGTCATCTTCGGGCAATTCCATGGGTGTTTGCATGGACTCAAACCAGATTTGTTCTTCCAGCTTGGCTTGGAGTTGGACCAGGTTTAAGGGGGGTTTGTGAGAAGGGACACACTGAAGACTTGAAAGCCATGTACAAAGAATGGCCTTTCTTCCAGTCCACCATTGACCTCATTGAGATGGTTTTAGGGAAGGCGGACGTTCCTATATCCAAACACTATGATGAAGTTCTCGTCTCAAACAGCAGGCGCCATCTTGGTGATGAACTGAGAATGGAGCTGGTAGCGACAGAGAAATTGGTGTTGGTGGTTAGTGGACATGAGAAACTAGCTGCTAATAATCGGATCTTGAGGAGGCTGATCGAGAGCAGGCTTGCCTATCTCAATCCCATGAACTTATTGCAGGTTGAGGTACTTAAGAGATTGAGAAGCGATGATGATAATAAACAACTCAGAGATGCCTTGCTAATCACAATCAACGGGATCGCTGCAGGGATGAGGAATACAGGCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004611 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006082 GO:0006091 GO:0006099 GO:0006101 GO:0008150 GO:0008152 GO:0008964 GO:0009060 GO:0009987 GO:0015980 GO:0016829 GO:0016830 GO:0016831 GO:0016999 GO:0017144 GO:0019752 GO:0043436 GO:0044237 GO:0044238 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0045333 GO:0055114 GO:0071704 GO:0072350

KEGG Pathways

Metabolic & Signaling

ko00620 ko00680 ko00710 ko00720 ko01100 ko01120 ko01200 map00620 map00680 map00710 map00720 map01100 map01120 map01200

Pfam Domains

Protein Families

PEPcase

Protein Analysis

1030

Amino Acids

116.21

Weight (kDa)

7.0

Isoelectric Point (pI)

53.94

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PEPcase	PF00311	18 - 331	9.9e-84	Phosphoenolpyruvate carboxylase
PEPcase	PF00311	437 - 1030	1.2e-231	Phosphoenolpyruvate carboxylase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000999)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G68750
fragaria_vesca	FvH4_3g20961 FvH4_4g28350 FvH4_4g28350 FvH4_4g28350 FvH4_4g28350 FvH4_4g28350 FvH4_4g28350 FvH4_4g28350 FvH4_4g28350 FvH4_4g28350 FvH4_4g28350 FvH4_4g28350
malus_domestica	MD13G1049200.v1.1 MD16G1050300.v1.1
prunus_persica	Prupe.1G302700_v2.0.a1 Prupe.1G302700_v2.0.a1
pyrus_communis	pycom16g04410
rosa_chinensis	RchiOBHm_Chr1g0330331 RchiOBHm_Chr1g0355581 RchiOBHm_Chr1g0379911 RchiOBHm_Chr4g0407141 RchiOBHm_Chr4g0436201 RchiOBHm_Chr5g0035501 RchiOBHm_Chr5g0035601 RchiOBHm_Chr5g0035681 RchiOBHm_Chr5g0035861 RchiOBHm_Chr5g0063691 RchiOBHm_Chr6g0312331
rosa_laevigata	RLG00000006529 RLG00000033624 RLG00000033642 RLG00000033654
rosa_multiflora	Rmu_sc0000084.1_g000022 Rmu_sc0006173.1_g000008 Rmu_sc0007205.1_g000021 Rmu_sc0014259.1_g000014
rosa_roxburghii	Rroxscaffold_1G00021870 Rroxscaffold_1G00044830 Rroxscaffold_1G00044890 Rroxscaffold_1G00044970 Rroxscaffold_3G00231670 Rroxscaffold_5G00377400 Rroxscaffold_7G00185100
rosa_rugosa	Rorug03G0294000 Rorug04G0292500 Rorug04G0292600 Rorug04G0292600
rosa_samantha	Rh4AG346800 Rh4BG355700 Rh4CG370400 Rh4DG349400 Rh5AG245000 Rh5BG244800 Rh5CG276300 Rh5CG276800 Rh5DG253900 Rh5DG464900 Rh6BG126500
rosa_wichuraiana	Rw1G008440 Rw4G030310 Rw5G022500

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	650, 2974
AccB1I	GGYRCC	2 cut(s)	624, 2829
AccB7I	CCANNNNNTGG	2 cut(s)	1925, 2016
AccI	GTMKAC	4 cut(s)	132, 328, 1609, 2472
AccIII	TCCGGA	1 cut(s)	1174
AciI	CCGC	9 cut(s)	136, 179, 197, 1049, 1724, 1783, 2252, 2551, 2777
AclI	AACGTT	1 cut(s)	2378
AcoI	YGGCCR	2 cut(s)	277, 2235
AcsI	RAATTY	5 cut(s)	908, 1137, 1691, 2312, 2486
AcuI	CTGAAG	4 cut(s)	327, 573, 782, 2718
AcyI	GRCGYC	2 cut(s)	625, 2830
AfaI	GTAC	7 cut(s)	699, 1770, 2021, 2272, 2304, 2717, 2994
AfiI	CCNNNNNNNGG	7 cut(s)	927, 1003, 1148, 1864, 1925, 2016, 2428
AflII	CTTAAG	1 cut(s)	2996
AflIII	ACRYGT	1 cut(s)	1612
AgsI	TTSAA	8 cut(s)	575, 667, 914, 1136, 1619, 1958, 2243, 2707
AhdI	GACNNNNNGTC	1 cut(s)	1727
AjnI	CCWGG	7 cut(s)	84, 1269, 1924, 2254, 2502, 2517, 2662
AjuI	GAANNNNNNNTTGG	4 cut(s)	1092, 1124, 2867, 2899
AleI	CACNNNNGTG	1 cut(s)	763
AloI	GAACNNNNNNTCC	2 cut(s)	23, 55
Alw21I	GWGCWC	6 cut(s)	115, 192, 770, 1710, 2031, 2573
Alw26I	GTCTC	6 cut(s)	413, 853, 1209, 1241, 1735, 2821
Alw44I	GTGCAC	1 cut(s)	766
AlwNI	CAGNNNCTG	1 cut(s)	385
Ama87I	CYCGRG	1 cut(s)	2400
Aor13HI	TCCGGA	1 cut(s)	1174
AoxI	GGCC	4 cut(s)	277, 2221, 2235, 2726
ApaI	GGGCCC	1 cut(s)	2225
ApaLI	GTGCAC	1 cut(s)	766
ApoI	RAATTY	5 cut(s)	908, 1137, 1691, 2312, 2486
AseI	ATTAAT	2 cut(s)	474, 1577
AspLEI	GCGC	2 cut(s)	627, 2832
AspS9I	GGNCC	6 cut(s)	406, 1172, 1281, 2221, 2222, 2660
AsuC2I	CCSGG	1 cut(s)	404
AsuHPI	GGTGA	6 cut(s)	580, 812, 986, 1725, 2074, 2852
AsuII	TTCGAA	2 cut(s)	51, 233
AsuNHI	GCTAGC	2 cut(s)	175, 217
AvaI	CYCGRG	1 cut(s)	2400
AvaII	GGWCC	4 cut(s)	406, 1172, 1281, 2660
BaeGI	GKGCMC	3 cut(s)	770, 1658, 2225
BalI	TGGCCA	1 cut(s)	2237
BamHI	GGATCC	1 cut(s)	1515
BanI	GGYRCC	2 cut(s)	624, 2829
BanII	GRGCYC	4 cut(s)	115, 1710, 2225, 2573
BarI	GAAGNNNNNNTAC	2 cut(s)	1543, 1575
BauI	CACGAG	1 cut(s)	267
BbsI	GAAGAC	4 cut(s)	60, 1385, 1393, 2706
Bbv12I	GWGCWC	6 cut(s)	115, 192, 770, 1710, 2031, 2573
BceAI	ACGGC	1 cut(s)	1880
BciT130I	CCWGG	7 cut(s)	86, 1271, 1926, 2256, 2504, 2519, 2664
BciVI	GTATCC	2 cut(s)	1768, 2066
BclI	TGATCA	2 cut(s)	1081, 1992
BcnI	CCSGG	1 cut(s)	404
BcoDI	GTCTC	6 cut(s)	413, 853, 1209, 1241, 1735, 2821
BfmI	CTRYAG	4 cut(s)	139, 386, 2574, 3069
BfoI	RGCGCY	2 cut(s)	628, 2833
BfrI	CTTAAG	1 cut(s)	2996
BfuAI	ACCTGC	2 cut(s)	650, 2974
BfuI	GTATCC	2 cut(s)	1768, 2066
BglII	AGATCT	3 cut(s)	1187, 1322, 2433
BlpI	GCTNAGC	2 cut(s)	1794, 2523
BmcAI	AGTACT	1 cut(s)	1770
Bme1390I	CCNGG	8 cut(s)	86, 404, 1271, 1926, 2256, 2504, 2519, 2664
Bme18I	GGWCC	4 cut(s)	406, 1172, 1281, 2660
BmeRI	GACNNNNNGTC	1 cut(s)	1727
BmeT110I	CYCGRG	1 cut(s)	2400
BmgT120I	GGNCC	6 cut(s)	406, 1172, 1281, 2221, 2222, 2660
BmiI	GGNNCC	7 cut(s)	626, 1305, 1517, 1947, 2222, 2223, 2831
BmrFI	CCNGG	8 cut(s)	86, 404, 1271, 1926, 2256, 2504, 2519, 2664
BmrI	ACTGGG	1 cut(s)	1158
BmsI	GCATC	7 cut(s)	353, 992, 1618, 1873, 2062, 2083, 3028
BmtI	GCTAGC	2 cut(s)	179, 221
BmuI	ACTGGG	1 cut(s)	1158
BoxI	GACNNNNGTC	1 cut(s)	1593
BpiI	GAAGAC	4 cut(s)	60, 1385, 1393, 2706
BplI	GAGNNNNNCTC	4 cut(s)	20, 52, 2420, 2452
BpmI	CTGGAG	2 cut(s)	107, 227
Bpu10I	CCTNAGC	1 cut(s)	620
Bpu1102I	GCTNAGC	2 cut(s)	1794, 2523
Bpu14I	TTCGAA	2 cut(s)	51, 233
BpuEI	CTTGAG	3 cut(s)	1993, 2118, 2950
BpuMI	CCSGG	1 cut(s)	404
BsaBI	GATNNNNATC	2 cut(s)	813, 1428
BsaHI	GRCGYC	2 cut(s)	625, 2830
BsaI	GGTCTC	1 cut(s)	1209
BsaJI	CCNNGG	5 cut(s)	27, 402, 1269, 2517, 2603
BsaWI	WCCGGW	1 cut(s)	1174
BsaXI	ACNNNNNCTCC	4 cut(s)	1809, 1839, 2286, 2316
Bsc4I	CCNNNNNNNGG	7 cut(s)	927, 1003, 1148, 1864, 1925, 2016, 2428
Bse1I	ACTGG	8 cut(s)	641, 748, 957, 1153, 1284, 1692, 1776, 2737
Bse3DI	GCAATG	6 cut(s)	300, 730, 1111, 1239, 1785, 2164
Bse8I	GATNNNNATC	2 cut(s)	813, 1428
BseAI	TCCGGA	1 cut(s)	1174
BseBI	CCWGG	7 cut(s)	86, 1271, 1926, 2256, 2504, 2519, 2664
BseDI	CCNNGG	5 cut(s)	27, 402, 1269, 2517, 2603
BseJI	GATNNNNATC	2 cut(s)	813, 1428
BseLI	CCNNNNNNNGG	7 cut(s)	927, 1003, 1148, 1864, 1925, 2016, 2428
BseMI	GCAATG	6 cut(s)	300, 730, 1111, 1239, 1785, 2164
BseNI	ACTGG	8 cut(s)	641, 748, 957, 1153, 1284, 1692, 1776, 2737
BseRI	GAGGAG	6 cut(s)	263, 1992, 2211, 2388, 2444, 2947
BseSI	GKGCMC	3 cut(s)	770, 1658, 2225
BseYI	CCCAGC	1 cut(s)	2247
BsgI	GTGCAG	3 cut(s)	441, 678, 952
Bsh1285I	CGRYCG	1 cut(s)	136
BshFI	GGCC	4 cut(s)	279, 2223, 2237, 2728
BshNI	GGYRCC	2 cut(s)	624, 2829
BsiEI	CGRYCG	1 cut(s)	136
BsiHKAI	GWGCWC	6 cut(s)	115, 192, 770, 1710, 2031, 2573
BsiHKCI	CYCGRG	1 cut(s)	2400
BsiSI	CCGG	2 cut(s)	404, 1175
BslFI	GGGAC	3 cut(s)	667, 1252, 2703
BslI	CCNNNNNNNGG	7 cut(s)	927, 1003, 1148, 1864, 1925, 2016, 2428
BsmAI	GTCTC	6 cut(s)	413, 853, 1209, 1241, 1735, 2821
BsmBI	CGTCTC	1 cut(s)	2821
BsmFI	GGGAC	3 cut(s)	667, 1252, 2703
BsmI	GAATGC	1 cut(s)	1106
BsnI	GGCC	4 cut(s)	279, 2223, 2237, 2728
Bso31I	GGTCTC	1 cut(s)	1209
BsoBI	CYCGRG	1 cut(s)	2400
Bsp119I	TTCGAA	2 cut(s)	51, 233
Bsp120I	GGGCCC	1 cut(s)	2221
Bsp1286I	GDGCHC	8 cut(s)	115, 192, 770, 1658, 1710, 2031, 2225, 2573
Bsp13I	TCCGGA	1 cut(s)	1174
Bsp1407I	TGTACA	1 cut(s)	2715
Bsp1720I	GCTNAGC	2 cut(s)	1794, 2523
Bsp19I	CCATGG	1 cut(s)	2603
BspACI	CCGC	9 cut(s)	136, 179, 197, 1049, 1724, 1783, 2252, 2551, 2777
BspANI	GGCC	4 cut(s)	279, 2223, 2237, 2728
BspEI	TCCGGA	1 cut(s)	1174
BspLI	GGNNCC	7 cut(s)	626, 1305, 1517, 1947, 2222, 2223, 2831
BspMAI	CTGCAG	2 cut(s)	390, 3073
BspMI	ACCTGC	2 cut(s)	650, 2974
BspOI	GCTAGC	2 cut(s)	179, 221
BspT104I	TTCGAA	2 cut(s)	51, 233
BspT107I	GGYRCC	2 cut(s)	624, 2829
BspTI	CTTAAG	1 cut(s)	2996
BspTNI	GGTCTC	1 cut(s)	1209
BsrDI	GCAATG	6 cut(s)	300, 730, 1111, 1239, 1785, 2164
BsrGI	TGTACA	1 cut(s)	2715
BsrI	ACTGG	8 cut(s)	641, 748, 957, 1153, 1284, 1692, 1776, 2737
BssECI	CCNNGG	5 cut(s)	27, 402, 1269, 2517, 2603
BssNI	GRCGYC	2 cut(s)	625, 2830
BssSI	CACGAG	1 cut(s)	267
BssT1I	CCWWGG	1 cut(s)	2603
Bst2BI	CACGAG	1 cut(s)	267
Bst2UI	CCWGG	7 cut(s)	86, 1271, 1926, 2256, 2504, 2519, 2664
Bst4CI	ACNGT	3 cut(s)	424, 1664, 1963
Bst6I	CTCTTC	3 cut(s)	549, 1227, 1546
BstACI	GRCGYC	2 cut(s)	625, 2830
BstAFI	CTTAAG	1 cut(s)	2996
BstAPI	GCANNNNNTGC	2 cut(s)	1101, 1892
BstAUI	TGTACA	1 cut(s)	2715
BstBI	TTCGAA	2 cut(s)	51, 233
BstDSI	CCRYGG	1 cut(s)	2603
BstENI	CCTNNNNNAGG	1 cut(s)	2426
BstH2I	RGCGCY	2 cut(s)	628, 2833
BstHHI	GCGC	2 cut(s)	627, 2832
BstMAI	GTCTC	6 cut(s)	413, 853, 1209, 1241, 1735, 2821
BstMCI	CGRYCG	1 cut(s)	136
BstMWI	GCNNNNNNNGC	9 cut(s)	158, 211, 388, 939, 1101, 1303, 1591, 1844, 1892
BstNI	CCWGG	7 cut(s)	86, 1271, 1926, 2256, 2504, 2519, 2664
BstNSI	RCATGY	3 cut(s)	1485, 1616, 2157
BstPAI	GACNNNNGTC	1 cut(s)	1593
BstSCI	CCNGG	8 cut(s)	84, 402, 1269, 1924, 2254, 2502, 2517, 2662
BstSFI	CTRYAG	4 cut(s)	139, 386, 2574, 3069
BstSLI	GKGCMC	3 cut(s)	770, 1658, 2225
BstV2I	GAAGAC	4 cut(s)	60, 1385, 1393, 2706
BstX2I	RGATCY	8 cut(s)	963, 1187, 1322, 1411, 1508, 1515, 2433, 2925
BstXI	CCANNNNNNTGG	2 cut(s)	1645, 2255
BstYI	RGATCY	8 cut(s)	963, 1187, 1322, 1411, 1508, 1515, 2433, 2925
BsuI	GTATCC	2 cut(s)	1768, 2066
BsuRI	GGCC	4 cut(s)	279, 2223, 2237, 2728
BtgI	CCRYGG	1 cut(s)	2603
BtgZI	GCGATG	1 cut(s)	3027
BtsIMutI	CAGTG	8 cut(s)	741, 758, 1019, 1095, 1472, 1669, 1995, 2694
BveI	ACCTGC	2 cut(s)	650, 2974
CaiI	CAGNNNCTG	1 cut(s)	385
CfoI	GCGC	2 cut(s)	627, 2832
Cfr13I	GGNCC	6 cut(s)	406, 1172, 1281, 2221, 2222, 2660
CseI	GACGC	3 cut(s)	210, 1583, 2372
CsiI	ACCWGGT	1 cut(s)	2662
Csp6I	GTAC	7 cut(s)	698, 1769, 2020, 2271, 2303, 2716, 2993
CspCI	CAANNNNNGTGG	2 cut(s)	625, 660
CviQI	GTAC	7 cut(s)	698, 1769, 2020, 2271, 2303, 2716, 2993
DinI	GGCGCC	2 cut(s)	626, 2831
DraI	TTTAAA	2 cut(s)	528, 732
DriI	GACNNNNNGTC	1 cut(s)	1727
EaeI	YGGCCR	2 cut(s)	277, 2235
Eam1104I	CTCTTC	3 cut(s)	549, 1227, 1546
Eam1105I	GACNNNNNGTC	1 cut(s)	1727
EarI	CTCTTC	3 cut(s)	549, 1227, 1546
EciI	GGCGGA	2 cut(s)	212, 2792
Ecl136II	GAGCTC	3 cut(s)	113, 1708, 2571
Eco130I	CCWWGG	1 cut(s)	2603
Eco24I	GRGCYC	4 cut(s)	115, 1710, 2225, 2573
Eco31I	GGTCTC	1 cut(s)	1209
Eco32I	GATATC	2 cut(s)	22, 2170
Eco47I	GGWCC	4 cut(s)	406, 1172, 1281, 2660
Eco53kI	GAGCTC	3 cut(s)	113, 1708, 2571
Eco57I	CTGAAG	4 cut(s)	327, 573, 782, 2718
Eco88I	CYCGRG	1 cut(s)	2400
EcoICRI	GAGCTC	3 cut(s)	113, 1708, 2571
EcoNI	CCTNNNNNAGG	1 cut(s)	2426
EcoO109I	RGGNCCY	2 cut(s)	2221, 2222
EcoRI	GAATTC	1 cut(s)	2486
EcoRII	CCWGG	7 cut(s)	84, 1269, 1924, 2254, 2502, 2517, 2662
EcoRV	GATATC	2 cut(s)	22, 2170
EcoT14I	CCWWGG	1 cut(s)	2603
EcoT38I	GRGCYC	4 cut(s)	115, 1710, 2225, 2573
EgeI	GGCGCC	2 cut(s)	626, 2831
EheI	GGCGCC	2 cut(s)	626, 2831
ErhI	CCWWGG	1 cut(s)	2603
Esp3I	CGTCTC	1 cut(s)	2821
FalI	AAGNNNNNCTT	4 cut(s)	1356, 1388, 1574, 1606
FaqI	GGGAC	3 cut(s)	667, 1252, 2703
FauI	CCCGC	1 cut(s)	172
FauNDI	CATATG	1 cut(s)	1822
FbaI	TGATCA	2 cut(s)	1081, 1992
FblI	GTMKAC	4 cut(s)	132, 328, 1609, 2472
FriOI	GRGCYC	4 cut(s)	115, 1710, 2225, 2573
GlaI	GCGC	2 cut(s)	626, 2831
GsaI	CCCAGC	1 cut(s)	2251
GsuI	CTGGAG	2 cut(s)	107, 227
HaeII	RGCGCY	2 cut(s)	628, 2833
HaeIII	GGCC	4 cut(s)	279, 2223, 2237, 2728
HapII	CCGG	2 cut(s)	404, 1175
HgaI	GACGC	3 cut(s)	210, 1583, 2372
HhaI	GCGC	2 cut(s)	627, 2832
Hin1I	GRCGYC	2 cut(s)	625, 2830
Hin6I	GCGC	2 cut(s)	625, 2830
HinP1I	GCGC	2 cut(s)	625, 2830
HincII	GTYRAC	1 cut(s)	133
HindII	GTYRAC	1 cut(s)	133
HindIII	AAGCTT	2 cut(s)	1295, 1370
HinfI	GANTC	8 cut(s)	977, 1178, 1265, 1490, 1601, 1808, 2081, 2620
HpaII	CCGG	2 cut(s)	404, 1175
HphI	GGTGA	6 cut(s)	580, 812, 986, 1725, 2074, 2852
Hpy166II	GTNNAC	7 cut(s)	133, 329, 768, 1610, 2473, 2742, 2897
Hpy8I	GTNNAC	7 cut(s)	133, 329, 768, 1610, 2473, 2742, 2897
Hpy99I	CGWCG	2 cut(s)	16, 717
HpyAV	CCTTC	5 cut(s)	1272, 1789, 1960, 2680, 2767
HpyCH4III	ACNGT	3 cut(s)	424, 1664, 1963
HpyCH4IV	ACGT	3 cut(s)	712, 2378, 2781
HpyF10VI	GCNNNNNNNGC	9 cut(s)	158, 211, 388, 939, 1101, 1303, 1591, 1844, 1892
HpySE526I	ACGT	3 cut(s)	712, 2378, 2781
Hsp92I	GRCGYC	2 cut(s)	625, 2830
HspAI	GCGC	2 cut(s)	625, 2830
KasI	GGCGCC	2 cut(s)	624, 2829
Kpn2I	TCCGGA	1 cut(s)	1174
Ksp22I	TGATCA	2 cut(s)	1081, 1992
LweI	GCATC	7 cut(s)	353, 992, 1618, 1873, 2062, 2083, 3028
MabI	ACCWGGT	1 cut(s)	2662
MaeII	ACGT	3 cut(s)	712, 2378, 2781
MaeIII	GTNAC	4 cut(s)	823, 835, 2176, 2417
MfeI	CAATTG	1 cut(s)	1329
MflI	RGATCY	8 cut(s)	963, 1187, 1322, 1411, 1508, 1515, 2433, 2925
MhlI	GDGCHC	8 cut(s)	115, 192, 770, 1658, 1710, 2031, 2225, 2573
MlsI	TGGCCA	1 cut(s)	2237
MluNI	TGGCCA	1 cut(s)	2237
Mly113I	GGCGCC	2 cut(s)	625, 2830
MlyI	GAGTC	4 cut(s)	1172, 1274, 1484, 2614
MmeI	TCCRAC	6 cut(s)	88, 249, 1082, 1255, 2050, 2638
Mox20I	TGGCCA	1 cut(s)	2237
MroI	TCCGGA	1 cut(s)	1174
MscI	TGGCCA	1 cut(s)	2237
MseI	TTAA	8 cut(s)	474, 527, 731, 1428, 1577, 1761, 2669, 2997
MslI	CAYNNNNRTG	4 cut(s)	763, 2759, 2802, 2838
Msp20I	TGGCCA	1 cut(s)	2237
MspA1I	CMGCKG	1 cut(s)	2363
MspCI	CTTAAG	1 cut(s)	2996
MspI	CCGG	2 cut(s)	404, 1175
MspR9I	CCNGG	8 cut(s)	86, 404, 1271, 1926, 2256, 2504, 2519, 2664
MunI	CAATTG	1 cut(s)	1329
Mva1269I	GAATGC	1 cut(s)	1106
MvaI	CCWGG	7 cut(s)	86, 1271, 1926, 2256, 2504, 2519, 2664
MwoI	GCNNNNNNNGC	9 cut(s)	158, 211, 388, 939, 1101, 1303, 1591, 1844, 1892
NarI	GGCGCC	2 cut(s)	625, 2830
NciI	CCSGG	1 cut(s)	404
NcoI	CCATGG	1 cut(s)	2603
NdeI	CATATG	1 cut(s)	1822
NheI	GCTAGC	2 cut(s)	175, 217
NlaIV	GGNNCC	7 cut(s)	626, 1305, 1517, 1947, 2222, 2223, 2831
NmeAIII	GCCGAG	2 cut(s)	51, 52
NmuCI	GTSAC	2 cut(s)	823, 835
NspI	RCATGY	3 cut(s)	1485, 1616, 2157
NspV	TTCGAA	2 cut(s)	51, 233
OliI	CACNNNNGTG	1 cut(s)	763
PaeI	GCATGC	1 cut(s)	2157
PaeR7I	CTCGAG	1 cut(s)	2400
PciI	ACATGT	1 cut(s)	1612
PcsI	WCGNNNNNNNCGW	1 cut(s)	24
PctI	GAATGC	1 cut(s)	1106
PfeI	GAWTC	4 cut(s)	977, 1601, 1808, 2081
PflMI	CCANNNNNTGG	2 cut(s)	1925, 2016
PfoI	TCCNGGA	2 cut(s)	84, 2502
PleI	GAGTC	4 cut(s)	1172, 1273, 1484, 2614
PluTI	GGCGCC	2 cut(s)	628, 2833
PpsI	GAGTC	4 cut(s)	1172, 1273, 1484, 2614
PscI	ACATGT	1 cut(s)	1612
PshAI	GACNNNNGTC	1 cut(s)	1593
PshBI	ATTAAT	2 cut(s)	474, 1577
Psp124BI	GAGCTC	3 cut(s)	115, 1710, 2573
Psp1406I	AACGTT	1 cut(s)	2378
Psp6I	CCWGG	7 cut(s)	84, 1269, 1924, 2254, 2502, 2517, 2662
PspFI	CCCAGC	1 cut(s)	2247
PspGI	CCWGG	7 cut(s)	84, 1269, 1924, 2254, 2502, 2517, 2662
PspN4I	GGNNCC	7 cut(s)	626, 1305, 1517, 1947, 2222, 2223, 2831
PspOMI	GGGCCC	1 cut(s)	2221
PspPI	GGNCC	6 cut(s)	406, 1172, 1281, 2221, 2222, 2660
PstI	CTGCAG	2 cut(s)	390, 3073
PstNI	CAGNNNCTG	1 cut(s)	385
PsuI	RGATCY	8 cut(s)	963, 1187, 1322, 1411, 1508, 1515, 2433, 2925
PvuII	CAGCTG	1 cut(s)	2363
RsaI	GTAC	7 cut(s)	699, 1770, 2021, 2272, 2304, 2717, 2994
RsaNI	GTAC	7 cut(s)	698, 1769, 2020, 2271, 2303, 2716, 2993
RseI	CAYNNNNRTG	4 cut(s)	763, 2759, 2802, 2838
SacI	GAGCTC	3 cut(s)	115, 1710, 2573
SalI	GTCGAC	1 cut(s)	131
SaqAI	TTAA	8 cut(s)	474, 527, 731, 1428, 1577, 1761, 2669, 2997
Sau96I	GGNCC	6 cut(s)	406, 1172, 1281, 2221, 2222, 2660
ScaI	AGTACT	1 cut(s)	1770
SchI	GAGTC	4 cut(s)	1172, 1274, 1484, 2614
ScrFI	CCNGG	8 cut(s)	86, 404, 1271, 1926, 2256, 2504, 2519, 2664
SduI	GDGCHC	8 cut(s)	115, 192, 770, 1658, 1710, 2031, 2225, 2573
SexAI	ACCWGGT	1 cut(s)	2662
SfaNI	GCATC	7 cut(s)	353, 992, 1618, 1873, 2062, 2083, 3028
SfcI	CTRYAG	4 cut(s)	139, 386, 2574, 3069
SfoI	GGCGCC	2 cut(s)	626, 2831
Sfr274I	CTCGAG	1 cut(s)	2400
SfuI	TTCGAA	2 cut(s)	51, 233
SinI	GGWCC	4 cut(s)	406, 1172, 1281, 2660
SlaI	CTCGAG	1 cut(s)	2400
SmiMI	CAYNNNNRTG	4 cut(s)	763, 2759, 2802, 2838
SmlI	CTYRAG	5 cut(s)	1972, 2133, 2400, 2929, 2996
SmoI	CTYRAG	5 cut(s)	1972, 2133, 2400, 2929, 2996
SphI	GCATGC	1 cut(s)	2157
SsiI	CCGC	9 cut(s)	136, 179, 197, 1049, 1724, 1783, 2252, 2551, 2777
SspDI	GGCGCC	2 cut(s)	624, 2829
SspI	AATATT	1 cut(s)	1643
SstI	GAGCTC	3 cut(s)	115, 1710, 2573
StyD4I	CCNGG	8 cut(s)	84, 402, 1269, 1924, 2254, 2502, 2517, 2662
StyI	CCWWGG	1 cut(s)	2603
TaaI	ACNGT	3 cut(s)	424, 1664, 1963
TaiI	ACGT	3 cut(s)	715, 2381, 2784
TaqI	TCGA	8 cut(s)	51, 132, 233, 889, 1144, 1587, 2401, 2943
TaqII	GACCGA	1 cut(s)	549
TatI	WGTACW	2 cut(s)	1768, 2715
TauI	GCSGC	3 cut(s)	1052, 2254, 2553
TfiI	GAWTC	4 cut(s)	977, 1601, 1808, 2081
Tru1I	TTAA	8 cut(s)	474, 527, 731, 1428, 1577, 1761, 2669, 2997
Tru9I	TTAA	8 cut(s)	474, 527, 731, 1428, 1577, 1761, 2669, 2997
TscAI	CASTG	8 cut(s)	748, 765, 1019, 1102, 1479, 1669, 1995, 2701
TseFI	GTSAC	2 cut(s)	823, 835
Tsp45I	GTSAC	2 cut(s)	823, 835
TspGWI	ACGGA	2 cut(s)	20, 29
TspRI	CASTG	8 cut(s)	748, 765, 1019, 1102, 1479, 1669, 1995, 2701
Van91I	CCANNNNNTGG	2 cut(s)	1925, 2016
Vha464I	CTTAAG	1 cut(s)	2996
VneI	GTGCAC	1 cut(s)	766
VpaK11BI	GGWCC	4 cut(s)	406, 1172, 1281, 2660
VspI	ATTAAT	2 cut(s)	474, 1577
XagI	CCTNNNNNAGG	1 cut(s)	2426
XapI	RAATTY	5 cut(s)	908, 1137, 1691, 2312, 2486
XbaI	TCTAGA	1 cut(s)	1405
XceI	RCATGY	3 cut(s)	1485, 1616, 2157
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2525
XhoI	CTCGAG	1 cut(s)	2400
XmiI	GTMKAC	4 cut(s)	132, 328, 1609, 2472
ZrmI	AGTACT	1 cut(s)	1770

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.