Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr7

Orthogroup ID

OG0000999

Physical Location & Seq

Genomic Coordinates Forward (+)

29720875 .. 29727267

Gene Structure 6393 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000033624

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1257 bp

ATGGTAGTGAGTGGTATATCAAATATGGTAGTGAGTGGTATGTTTGATATGGGCACATGCAGTGAGTGGGATGAAGAAAAGAAACTGGAATTTCTAACAAGAGAGCTCAAAGGAAAAAGACAGCTAGTCCCGCCTAGCATGGAGGCAAGTGATGTCCTTGCTGTGGAGCTTTTGCAAAAAGATGCACGACTTGCCGTTAGTGGAGAGATAGGAAAGCTGCTGCGTGTGGTTCCTTTGTTTGAAACTGTAAAGGACTTGAGAGGAGCTGGCTCAATGATCAGGAAATTATTGTCCATTGATTGGTACAGGGAGCACATCATCAAGAACCATAACGGGCATAAAGAGATATCTATAGATGTTGTAGCTGCATGCAATGAATATGATATCAAAATTACCCTGTTTCATGGGAGAGGAGGAAGTATTGGTCGTGGAGGGGGCCCTACATATCTTGCCATTCAGTCCCAGCCGCCTGGCTCTGTGATGGAGAAGTATTCACCAATTCGCAAGTTAACAGCTCCTTATGTCTTGAACTTGGACGTTATTGATATGCTAGTGGTACTCTATGATCAACTGAGCAAGGAGAAATGGTACAGGCAAAAATTTGGGCTGCCGGAGATTGTTGTCAGACAGCTAGAGATATACACAACAGCTGCGCTGCTTACAACGTTGCGTCCACCACACCCTTCTCGAGAACAAAAGTCGGGTATCCTCATGGAGGAGATCACAAAGATCAGTTGTCAGAATTACAGAAGTGGTCTACGAAAATCCAAAATTCCTTACTTCCAGGAGGCTACACCCCAGGCTGAGCTTGGCTTCCTCAACATAGGAAGCCGCCCCTCAAGAAGAAAGAGCTCTACTGGAATTGGACATCTTCGGGCAATTCCATGGGTGTTTGCATGGACTCAAACCAGATTTGTTCTTCCAGCTTGGCTTGGAGTTGAACCAAGTTTGAGGGGGGTTTGTGGGAAGGGACACACTGAAGACTTGAAAGCAGAACATTATGAGCAAAATGGTCAAAATGCGAAGCCTAAGCCTCTCACAGTCCAGCTTCTCACTTCTATTCTTTCTTCCTCGATATATCTCTTCATCACTGTTCTCCGCTTCACCAACTCCACCCCTAATTCTCTCTACCTCTACTCCGATCTCCAAATCTCATCACAACTTCAACAGCATCTCCCTCACAATAGCGATACTTCGAGGCATCTGCCTCAGTCTCCCTCTGTTCCACCGCGCCCCTCCACTCCACTGCTCCTCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004611 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006082 GO:0006091 GO:0006099 GO:0006101 GO:0008150 GO:0008152 GO:0008964 GO:0009060 GO:0009987 GO:0015980 GO:0016829 GO:0016830 GO:0016831 GO:0016999 GO:0017144 GO:0019752 GO:0043436 GO:0044237 GO:0044238 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0045333 GO:0055114 GO:0071704 GO:0072350

KEGG Pathways

Metabolic & Signaling

ko00620 ko00680 ko00710 ko00720 ko01100 ko01120 ko01200 map00620 map00680 map00710 map00720 map01100 map01120 map01200

Pfam Domains

Protein Families

PEPcase

Protein Analysis

419

Amino Acids

46.9

Weight (kDa)

9.35

Isoelectric Point (pI)

47.44

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PEPcase	PF00311	47 - 114	6e-15	Phosphoenolpyruvate carboxylase
PEPcase	PF00311	120 - 161	1.2e-15	Phosphoenolpyruvate carboxylase
PEPcase	PF00311	198 - 329	1.8e-41	Phosphoenolpyruvate carboxylase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000999)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G68750
fragaria_vesca	FvH4_3g20961 FvH4_4g28350 FvH4_4g28350 FvH4_4g28350 FvH4_4g28350 FvH4_4g28350 FvH4_4g28350 FvH4_4g28350 FvH4_4g28350 FvH4_4g28350 FvH4_4g28350 FvH4_4g28350
malus_domestica	MD13G1049200.v1.1 MD16G1050300.v1.1
prunus_persica	Prupe.1G302700_v2.0.a1 Prupe.1G302700_v2.0.a1
pyrus_communis	pycom16g04410
rosa_chinensis	RchiOBHm_Chr1g0330331 RchiOBHm_Chr1g0355581 RchiOBHm_Chr1g0379911 RchiOBHm_Chr4g0407141 RchiOBHm_Chr4g0436201 RchiOBHm_Chr5g0035501 RchiOBHm_Chr5g0035601 RchiOBHm_Chr5g0035681 RchiOBHm_Chr5g0035861 RchiOBHm_Chr5g0063691 RchiOBHm_Chr6g0312331
rosa_laevigata	RLG00000006529 RLG00000033624 RLG00000033642 RLG00000033654
rosa_multiflora	Rmu_sc0000084.1_g000022 Rmu_sc0006173.1_g000008 Rmu_sc0007205.1_g000021 Rmu_sc0014259.1_g000014
rosa_roxburghii	Rroxscaffold_1G00021870 Rroxscaffold_1G00044830 Rroxscaffold_1G00044890 Rroxscaffold_1G00044970 Rroxscaffold_3G00231670 Rroxscaffold_5G00377400 Rroxscaffold_7G00185100
rosa_rugosa	Rorug03G0294000 Rorug04G0292500 Rorug04G0292600 Rorug04G0292600
rosa_samantha	Rh4AG346800 Rh4BG355700 Rh4CG370400 Rh4DG349400 Rh5AG245000 Rh5BG244800 Rh5CG276300 Rh5CG276800 Rh5DG253900 Rh5DG464900 Rh6BG126500
rosa_wichuraiana	Rw1G008440 Rw4G030310 Rw5G022500

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	300
AccI	GTMKAC	1 cut(s)	757
AccII	CGCG	1 cut(s)	1231
AciI	CCGC	5 cut(s)	131, 467, 832, 1099, 1229
AclI	AACGTT	1 cut(s)	665
AcsI	RAATTY	3 cut(s)	89, 599, 771
AcuI	CTGAAG	1 cut(s)	999
AfaI	GTAC	3 cut(s)	305, 558, 590
AfiI	CCNNNNNNNGG	3 cut(s)	163, 300, 715
AgsI	TTSAA	5 cut(s)	242, 529, 941, 988, 1166
AhdI	GACNNNNNGTC	1 cut(s)	125
AjnI	CCWGG	3 cut(s)	469, 783, 798
Alw21I	GWGCWC	3 cut(s)	108, 315, 854
Alw26I	GTCTC	1 cut(s)	1218
Ama87I	CYCGRG	1 cut(s)	687
AoxI	GGCC	1 cut(s)	436
ApaI	GGGCCC	1 cut(s)	440
ApeKI	GCWGC	6 cut(s)	217, 220, 365, 607, 650, 655
ApoI	RAATTY	3 cut(s)	89, 599, 771
AspLEI	GCGC	2 cut(s)	655, 1233
AspS9I	GGNCC	2 cut(s)	436, 437
AsuHPI	GGTGA	2 cut(s)	486, 1096
AvaI	CYCGRG	1 cut(s)	687
BaeGI	GKGCMC	2 cut(s)	56, 440
BanII	GRGCYC	3 cut(s)	108, 440, 854
BbsI	GAAGAC	1 cut(s)	987
Bbv12I	GWGCWC	3 cut(s)	108, 315, 854
BbvI	GCAGC	6 cut(s)	204, 207, 352, 594, 637, 642
BccI	CCATC	1 cut(s)	475
BceAI	ACGGC	1 cut(s)	179
BcgI	CGANNNNNNTGC	2 cut(s)	1186, 1220
BciT130I	CCWGG	3 cut(s)	471, 785, 800
BciVI	GTATCC	1 cut(s)	716
BclI	TGATCA	2 cut(s)	276, 565
BcoDI	GTCTC	1 cut(s)	1218
BfaI	CTAG	5 cut(s)	125, 135, 551, 632, 1255
BfmI	CTRYAG	1 cut(s)	351
BfuI	GTATCC	1 cut(s)	716
BisI	GCNGC	8 cut(s)	218, 221, 366, 467, 608, 651, 656, 832
BlpI	GCTNAGC	1 cut(s)	804
BlsI	GCNGC	8 cut(s)	219, 222, 367, 468, 609, 652, 657, 833
Bme1390I	CCNGG	3 cut(s)	471, 785, 800
BmeRI	GACNNNNNGTC	1 cut(s)	125
BmeT110I	CYCGRG	1 cut(s)	687
BmgT120I	GGNCC	2 cut(s)	436, 437
BmiI	GGNNCC	3 cut(s)	231, 437, 438
BmrFI	CCNGG	3 cut(s)	471, 785, 800
BmsI	GCATC	3 cut(s)	172, 1180, 1210
BpiI	GAAGAC	1 cut(s)	987
Bpu10I	CCTNAGC	1 cut(s)	1029
Bpu1102I	GCTNAGC	1 cut(s)	804
BpuEI	CTTGAG	2 cut(s)	277, 823
BsaJI	CCNNGG	2 cut(s)	798, 884
BsaXI	ACNNNNNCTCC	6 cut(s)	572, 602, 1121, 1151, 1158, 1188
Bsc4I	CCNNNNNNNGG	3 cut(s)	163, 300, 715
Bse1I	ACTGG	2 cut(s)	90, 862
Bse3DI	GCAATG	1 cut(s)	379
BseBI	CCWGG	3 cut(s)	471, 785, 800
BseDI	CCNNGG	2 cut(s)	798, 884
BseGI	GGATG	1 cut(s)	76
BseLI	CCNNNNNNNGG	3 cut(s)	163, 300, 715
BseMI	GCAATG	1 cut(s)	379
BseMII	CTCAG	3 cut(s)	563, 795, 1223
BseNI	ACTGG	2 cut(s)	90, 862
BseRI	GAGGAG	4 cut(s)	276, 426, 731, 1241
BseSI	GKGCMC	2 cut(s)	56, 440
BseXI	GCAGC	6 cut(s)	204, 207, 352, 594, 637, 642
BseYI	CCCAGC	1 cut(s)	462
Bsh1236I	CGCG	1 cut(s)	1231
BshFI	GGCC	1 cut(s)	438
BsiHKAI	GWGCWC	3 cut(s)	108, 315, 854
BsiHKCI	CYCGRG	1 cut(s)	687
BsiSI	CCGG	1 cut(s)	611
BslFI	GGGAC	3 cut(s)	113, 445, 984
BslI	CCNNNNNNNGG	3 cut(s)	163, 300, 715
BsmAI	GTCTC	1 cut(s)	1218
BsmFI	GGGAC	3 cut(s)	113, 445, 984
BsnI	GGCC	1 cut(s)	438
BsoBI	CYCGRG	1 cut(s)	687
Bsp120I	GGGCCC	1 cut(s)	436
Bsp1286I	GDGCHC	5 cut(s)	56, 108, 315, 440, 854
Bsp143I	GATC	5 cut(s)	276, 565, 720, 729, 1141
Bsp1720I	GCTNAGC	1 cut(s)	804
Bsp19I	CCATGG	1 cut(s)	884
BspACI	CCGC	5 cut(s)	131, 467, 832, 1099, 1229
BspANI	GGCC	1 cut(s)	438
BspCNI	CTCAG	3 cut(s)	564, 796, 1222
BspFNI	CGCG	1 cut(s)	1231
BspLI	GGNNCC	3 cut(s)	231, 437, 438
BsrDI	GCAATG	1 cut(s)	379
BsrI	ACTGG	2 cut(s)	90, 862
BssECI	CCNNGG	2 cut(s)	798, 884
BssMI	GATC	5 cut(s)	276, 565, 720, 729, 1141
BssT1I	CCWWGG	1 cut(s)	884
Bst2UI	CCWGG	3 cut(s)	471, 785, 800
Bst4CI	ACNGT	3 cut(s)	247, 1042, 1093
Bst6I	CTCTTC	1 cut(s)	1088
BstAPI	GCANNNNNTGC	1 cut(s)	191
BstC8I	GCNNGC	2 cut(s)	268, 370
BstDEI	CTNAG	4 cut(s)	572, 804, 1029, 1209
BstDSI	CCRYGG	1 cut(s)	884
BstENI	CCTNNNNNAGG	1 cut(s)	713
BstF5I	GGATG	1 cut(s)	76
BstFNI	CGCG	1 cut(s)	1231
BstHHI	GCGC	2 cut(s)	655, 1233
BstKTI	GATC	5 cut(s)	279, 568, 723, 732, 1144
BstMAI	GTCTC	1 cut(s)	1218
BstMBI	GATC	5 cut(s)	276, 565, 720, 729, 1141
BstMWI	GCNNNNNNNGC	2 cut(s)	130, 191
BstNI	CCWGG	3 cut(s)	471, 785, 800
BstNSI	RCATGY	2 cut(s)	60, 372
BstSCI	CCNGG	3 cut(s)	469, 783, 798
BstSFI	CTRYAG	1 cut(s)	351
BstSLI	GKGCMC	2 cut(s)	56, 440
BstUI	CGCG	1 cut(s)	1231
BstV1I	GCAGC	6 cut(s)	204, 207, 352, 594, 637, 642
BstV2I	GAAGAC	1 cut(s)	987
BstXI	CCANNNNNNTGG	1 cut(s)	470
BsuI	GTATCC	1 cut(s)	716
BsuRI	GGCC	1 cut(s)	438
BtgI	CCRYGG	1 cut(s)	884
BtsCI	GGATG	1 cut(s)	76
BtsI	GCAGTG	2 cut(s)	67, 1244
BtsIMutI	CAGTG	4 cut(s)	67, 975, 1089, 1244
Cac8I	GCNNGC	2 cut(s)	268, 370
CfoI	GCGC	2 cut(s)	655, 1233
Cfr13I	GGNCC	2 cut(s)	436, 437
CseI	GACGC	1 cut(s)	659
Csp6I	GTAC	3 cut(s)	304, 557, 589
CviAII	CATG	7 cut(s)	57, 139, 369, 404, 712, 885, 897
CviQI	GTAC	3 cut(s)	304, 557, 589
DdeI	CTNAG	4 cut(s)	572, 804, 1029, 1209
DpnI	GATC	5 cut(s)	278, 567, 722, 731, 1143
DpnII	GATC	5 cut(s)	276, 565, 720, 729, 1141
DriI	GACNNNNNGTC	1 cut(s)	125
Eam1104I	CTCTTC	1 cut(s)	1088
Eam1105I	GACNNNNNGTC	1 cut(s)	125
EarI	CTCTTC	1 cut(s)	1088
Ecl136II	GAGCTC	2 cut(s)	106, 852
Eco130I	CCWWGG	1 cut(s)	884
Eco24I	GRGCYC	3 cut(s)	108, 440, 854
Eco32I	GATATC	2 cut(s)	348, 385
Eco53kI	GAGCTC	2 cut(s)	106, 852
Eco57I	CTGAAG	1 cut(s)	999
Eco88I	CYCGRG	1 cut(s)	687
EcoICRI	GAGCTC	2 cut(s)	106, 852
EcoNI	CCTNNNNNAGG	1 cut(s)	713
EcoO109I	RGGNCCY	2 cut(s)	436, 437
EcoRII	CCWGG	3 cut(s)	469, 783, 798
EcoRV	GATATC	2 cut(s)	348, 385
EcoT14I	CCWWGG	1 cut(s)	884
EcoT38I	GRGCYC	3 cut(s)	108, 440, 854
ErhI	CCWWGG	1 cut(s)	884
FaeI	CATG	7 cut(s)	60, 142, 372, 407, 715, 888, 900
FaqI	GGGAC	3 cut(s)	113, 445, 984
FatI	CATG	7 cut(s)	56, 138, 368, 403, 711, 884, 896
FauI	CCCGC	1 cut(s)	138
FbaI	TGATCA	2 cut(s)	276, 565
FblI	GTMKAC	1 cut(s)	757
Fnu4HI	GCNGC	8 cut(s)	218, 221, 366, 467, 608, 651, 656, 832
FokI	GGATG	1 cut(s)	83
FriOI	GRGCYC	3 cut(s)	108, 440, 854
Fsp4HI	GCNGC	8 cut(s)	218, 221, 366, 467, 608, 651, 656, 832
FspBI	CTAG	5 cut(s)	125, 135, 551, 632, 1255
GlaI	GCGC	2 cut(s)	654, 1232
GluI	GCNGC	8 cut(s)	218, 221, 366, 467, 608, 651, 656, 832
GsaI	CCCAGC	1 cut(s)	466
HaeIII	GGCC	1 cut(s)	438
HapII	CCGG	1 cut(s)	611
HgaI	GACGC	1 cut(s)	659
HhaI	GCGC	2 cut(s)	655, 1233
Hin1II	CATG	7 cut(s)	60, 142, 372, 407, 715, 888, 900
Hin6I	GCGC	2 cut(s)	653, 1231
HinP1I	GCGC	2 cut(s)	653, 1231
HincII	GTYRAC	1 cut(s)	510
HindII	GTYRAC	1 cut(s)	510
HinfI	GANTC	1 cut(s)	901
HpaI	GTTAAC	1 cut(s)	510
HpaII	CCGG	1 cut(s)	611
HphI	GGTGA	2 cut(s)	486, 1096
Hpy166II	GTNNAC	3 cut(s)	510, 674, 758
Hpy188I	TCNGA	3 cut(s)	626, 741, 1141
Hpy188III	TCNNGA	6 cut(s)	280, 322, 526, 687, 689, 840
Hpy8I	GTNNAC	3 cut(s)	510, 674, 758
HpyAV	CCTTC	2 cut(s)	693, 961
HpyCH4III	ACNGT	3 cut(s)	247, 1042, 1093
HpyCH4IV	ACGT	2 cut(s)	537, 665
HpyCH4V	TGCA	6 cut(s)	60, 175, 185, 368, 372, 896
HpyF10VI	GCNNNNNNNGC	2 cut(s)	130, 191
HpyF3I	CTNAG	4 cut(s)	572, 804, 1029, 1209
HpySE526I	ACGT	2 cut(s)	537, 665
Hsp92II	CATG	7 cut(s)	60, 142, 372, 407, 715, 888, 900
HspAI	GCGC	2 cut(s)	653, 1231
Ksp22I	TGATCA	2 cut(s)	276, 565
KspAI	GTTAAC	1 cut(s)	510
Kzo9I	GATC	5 cut(s)	276, 565, 720, 729, 1141
LmnI	GCTCC	5 cut(s)	166, 263, 310, 520, 1254
Lsp1109I	GCAGC	6 cut(s)	204, 207, 352, 594, 637, 642
LweI	GCATC	3 cut(s)	172, 1180, 1210
MaeI	CTAG	5 cut(s)	125, 135, 551, 632, 1255
MaeII	ACGT	2 cut(s)	537, 665
MalI	GATC	5 cut(s)	278, 567, 722, 731, 1143
MboI	GATC	5 cut(s)	276, 565, 720, 729, 1141
MboII	GAAGA	7 cut(s)	86, 855, 863, 911, 992, 1059, 1075
MhlI	GDGCHC	5 cut(s)	56, 108, 315, 440, 854
MlyI	GAGTC	1 cut(s)	895
MseI	TTAA	1 cut(s)	509
MspA1I	CMGCKG	1 cut(s)	650
MspI	CCGG	1 cut(s)	611
MspR9I	CCNGG	3 cut(s)	471, 785, 800
MvaI	CCWGG	3 cut(s)	471, 785, 800
MvnI	CGCG	1 cut(s)	1231
MwoI	GCNNNNNNNGC	2 cut(s)	130, 191
NcoI	CCATGG	1 cut(s)	884
NdeII	GATC	5 cut(s)	276, 565, 720, 729, 1141
NlaIII	CATG	7 cut(s)	60, 142, 372, 407, 715, 888, 900
NlaIV	GGNNCC	3 cut(s)	231, 437, 438
NspI	RCATGY	2 cut(s)	60, 372
PaeI	GCATGC	1 cut(s)	372
PaeR7I	CTCGAG	1 cut(s)	687
PflMI	CCANNNNNTGG	1 cut(s)	300
PfoI	TCCNGGA	1 cut(s)	783
PkrI	GCNGC	8 cut(s)	219, 222, 367, 468, 609, 652, 657, 833
PleI	GAGTC	1 cut(s)	895
PpsI	GAGTC	1 cut(s)	895
Psp124BI	GAGCTC	2 cut(s)	108, 854
Psp1406I	AACGTT	1 cut(s)	665
Psp6I	CCWGG	3 cut(s)	469, 783, 798
PspFI	CCCAGC	1 cut(s)	462
PspGI	CCWGG	3 cut(s)	469, 783, 798
PspN4I	GGNNCC	3 cut(s)	231, 437, 438
PspOMI	GGGCCC	1 cut(s)	436
PspPI	GGNCC	2 cut(s)	436, 437
PvuII	CAGCTG	1 cut(s)	650
RsaI	GTAC	3 cut(s)	305, 558, 590
RsaNI	GTAC	3 cut(s)	304, 557, 589
SacI	GAGCTC	2 cut(s)	108, 854
SaqAI	TTAA	1 cut(s)	509
SatI	GCNGC	8 cut(s)	218, 221, 366, 467, 608, 651, 656, 832
Sau3AI	GATC	5 cut(s)	276, 565, 720, 729, 1141
Sau96I	GGNCC	2 cut(s)	436, 437
SchI	GAGTC	1 cut(s)	895
ScrFI	CCNGG	3 cut(s)	471, 785, 800
SduI	GDGCHC	5 cut(s)	56, 108, 315, 440, 854
SfaNI	GCATC	3 cut(s)	172, 1180, 1210
SfcI	CTRYAG	1 cut(s)	351
Sfr274I	CTCGAG	1 cut(s)	687
SlaI	CTCGAG	1 cut(s)	687
SmlI	CTYRAG	3 cut(s)	256, 687, 838
SmoI	CTYRAG	3 cut(s)	256, 687, 838
SphI	GCATGC	1 cut(s)	372
SsiI	CCGC	5 cut(s)	131, 467, 832, 1099, 1229
SspMI	CTAG	5 cut(s)	125, 135, 551, 632, 1255
SstI	GAGCTC	2 cut(s)	108, 854
StyD4I	CCNGG	3 cut(s)	469, 783, 798
StyI	CCWWGG	1 cut(s)	884
TaaI	ACNGT	3 cut(s)	247, 1042, 1093
TaiI	ACGT	2 cut(s)	540, 668
TaqI	TCGA	3 cut(s)	688, 1073, 1196
TauI	GCSGC	2 cut(s)	469, 834
Tru1I	TTAA	1 cut(s)	509
Tru9I	TTAA	1 cut(s)	509
TscAI	CASTG	4 cut(s)	67, 982, 1096, 1251
TseI	GCWGC	6 cut(s)	217, 220, 365, 607, 650, 655
TspDTI	ATGAA	4 cut(s)	87, 390, 392, 1075
TspRI	CASTG	4 cut(s)	67, 982, 1096, 1251
Van91I	CCANNNNNTGG	1 cut(s)	300
XagI	CCTNNNNNAGG	1 cut(s)	713
XapI	RAATTY	3 cut(s)	89, 599, 771
XceI	RCATGY	2 cut(s)	60, 372
XcmI	CCANNNNNNNNNTGG	1 cut(s)	806
XhoI	CTCGAG	1 cut(s)	687
XmiI	GTMKAC	1 cut(s)	757
XspI	CTAG	5 cut(s)	125, 135, 551, 632, 1255

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.