Phosphoenolpyruvate carboxylase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 2989 |
| AccB1I | GGYRCC | 2 cut(s) | 624, 2844 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 1940, 2031 |
| AccI | GTMKAC | 4 cut(s) | 132, 328, 1624, 2487 |
| AccII | CGCG | 1 cut(s) | 659 |
| AccIII | TCCGGA | 1 cut(s) | 1174 |
| AciI | CCGC | 9 cut(s) | 136, 179, 197, 659, 1049, 1739, 1798, 2267, 2566 |
| AcoI | YGGCCR | 1 cut(s) | 277 |
| AcsI | RAATTY | 5 cut(s) | 908, 1137, 1706, 2327, 2501 |
| AcuI | CTGAAG | 3 cut(s) | 327, 573, 2733 |
| AcyI | GRCGYC | 2 cut(s) | 625, 2845 |
| AfaI | GTAC | 7 cut(s) | 699, 1785, 2036, 2287, 2319, 2732, 3009 |
| AfiI | CCNNNNNNNGG | 7 cut(s) | 927, 1003, 1148, 1879, 1940, 2031, 2443 |
| AflII | CTTAAG | 1 cut(s) | 3011 |
| AgsI | TTSAA | 7 cut(s) | 575, 667, 914, 1136, 1973, 2258, 2722 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 1742 |
| AjnI | CCWGG | 7 cut(s) | 84, 1269, 1939, 2269, 2517, 2532, 2677 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 1092, 1124, 2882, 2914 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 23, 55 |
| Alw21I | GWGCWC | 4 cut(s) | 115, 192, 1725, 2046 |
| Alw26I | GTCTC | 6 cut(s) | 413, 853, 1209, 1241, 1750, 2836 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 385 |
| Ama87I | CYCGRG | 1 cut(s) | 2415 |
| Aor13HI | TCCGGA | 1 cut(s) | 1174 |
| AoxI | GGCC | 3 cut(s) | 277, 2236, 2741 |
| ApaI | GGGCCC | 1 cut(s) | 2240 |
| ApoI | RAATTY | 5 cut(s) | 908, 1137, 1706, 2327, 2501 |
| AseI | ATTAAT | 2 cut(s) | 474, 1577 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 2584 |
| AspLEI | GCGC | 3 cut(s) | 627, 659, 2847 |
| AspS9I | GGNCC | 6 cut(s) | 406, 1172, 1281, 2236, 2237, 2675 |
| AsuC2I | CCSGG | 1 cut(s) | 404 |
| AsuHPI | GGTGA | 5 cut(s) | 580, 812, 1740, 2089, 2867 |
| AsuII | TTCGAA | 2 cut(s) | 51, 233 |
| AsuNHI | GCTAGC | 3 cut(s) | 175, 217, 653 |
| AvaI | CYCGRG | 1 cut(s) | 2415 |
| AvaII | GGWCC | 4 cut(s) | 406, 1172, 1281, 2675 |
| BaeGI | GKGCMC | 2 cut(s) | 1673, 2240 |
| BamHI | GGATCC | 1 cut(s) | 1515 |
| BanI | GGYRCC | 2 cut(s) | 624, 2844 |
| BanII | GRGCYC | 3 cut(s) | 115, 1725, 2240 |
| BarI | GAAGNNNNNNTAC | 4 cut(s) | 1543, 1575, 2554, 2586 |
| BauI | CACGAG | 1 cut(s) | 267 |
| BbsI | GAAGAC | 4 cut(s) | 60, 1385, 1393, 2721 |
| Bbv12I | GWGCWC | 4 cut(s) | 115, 192, 1725, 2046 |
| BceAI | ACGGC | 1 cut(s) | 1895 |
| BciT130I | CCWGG | 7 cut(s) | 86, 1271, 1941, 2271, 2519, 2534, 2679 |
| BciVI | GTATCC | 2 cut(s) | 1783, 2081 |
| BclI | TGATCA | 2 cut(s) | 1081, 2007 |
| BcnI | CCSGG | 1 cut(s) | 404 |
| BcoDI | GTCTC | 6 cut(s) | 413, 853, 1209, 1241, 1750, 2836 |
| BfmI | CTRYAG | 4 cut(s) | 139, 386, 2589, 3084 |
| BfoI | RGCGCY | 2 cut(s) | 628, 2848 |
| BfrI | CTTAAG | 1 cut(s) | 3011 |
| BfuAI | ACCTGC | 1 cut(s) | 2989 |
| BfuI | GTATCC | 2 cut(s) | 1783, 2081 |
| BglII | AGATCT | 3 cut(s) | 1187, 1322, 2448 |
| BlpI | GCTNAGC | 2 cut(s) | 1809, 2538 |
| BmcAI | AGTACT | 1 cut(s) | 1785 |
| Bme1390I | CCNGG | 8 cut(s) | 86, 404, 1271, 1941, 2271, 2519, 2534, 2679 |
| Bme18I | GGWCC | 4 cut(s) | 406, 1172, 1281, 2675 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 1742 |
| BmeT110I | CYCGRG | 1 cut(s) | 2415 |
| BmgT120I | GGNCC | 6 cut(s) | 406, 1172, 1281, 2236, 2237, 2675 |
| BmiI | GGNNCC | 7 cut(s) | 626, 1517, 1962, 2237, 2238, 2549, 2846 |
| BmrFI | CCNGG | 8 cut(s) | 86, 404, 1271, 1941, 2271, 2519, 2534, 2679 |
| BmrI | ACTGGG | 1 cut(s) | 1158 |
| BmsI | GCATC | 7 cut(s) | 353, 992, 1633, 1888, 2077, 2098, 3043 |
| BmtI | GCTAGC | 3 cut(s) | 179, 221, 657 |
| BmuI | ACTGGG | 1 cut(s) | 1158 |
| BoxI | GACNNNNGTC | 1 cut(s) | 1593 |
| BpiI | GAAGAC | 4 cut(s) | 60, 1385, 1393, 2721 |
| BplI | GAGNNNNNCTC | 4 cut(s) | 20, 52, 2435, 2467 |
| BpmI | CTGGAG | 2 cut(s) | 107, 227 |
| Bpu10I | CCTNAGC | 1 cut(s) | 620 |
| Bpu1102I | GCTNAGC | 2 cut(s) | 1809, 2538 |
| Bpu14I | TTCGAA | 2 cut(s) | 51, 233 |
| BpuEI | CTTGAG | 3 cut(s) | 2008, 2133, 2965 |
| BpuMI | CCSGG | 1 cut(s) | 404 |
| BsaBI | GATNNNNATC | 2 cut(s) | 813, 1428 |
| BsaHI | GRCGYC | 2 cut(s) | 625, 2845 |
| BsaJI | CCNNGG | 5 cut(s) | 27, 402, 1269, 2532, 2618 |
| BsaWI | WCCGGW | 1 cut(s) | 1174 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 1824, 1854, 2301, 2331 |
| Bsc4I | CCNNNNNNNGG | 7 cut(s) | 927, 1003, 1148, 1879, 1940, 2031, 2443 |
| Bse1I | ACTGG | 8 cut(s) | 641, 748, 957, 1153, 1284, 1707, 1791, 2752 |
| Bse3DI | GCAATG | 7 cut(s) | 300, 730, 1111, 1239, 1800, 2179, 2393 |
| Bse8I | GATNNNNATC | 2 cut(s) | 813, 1428 |
| BseAI | TCCGGA | 1 cut(s) | 1174 |
| BseBI | CCWGG | 7 cut(s) | 86, 1271, 1941, 2271, 2519, 2534, 2679 |
| BseDI | CCNNGG | 5 cut(s) | 27, 402, 1269, 2532, 2618 |
| BseJI | GATNNNNATC | 2 cut(s) | 813, 1428 |
| BseLI | CCNNNNNNNGG | 7 cut(s) | 927, 1003, 1148, 1879, 1940, 2031, 2443 |
| BseMI | GCAATG | 7 cut(s) | 300, 730, 1111, 1239, 1800, 2179, 2393 |
| BseNI | ACTGG | 8 cut(s) | 641, 748, 957, 1153, 1284, 1707, 1791, 2752 |
| BseRI | GAGGAG | 6 cut(s) | 263, 2007, 2226, 2403, 2459, 2962 |
| BseSI | GKGCMC | 2 cut(s) | 1673, 2240 |
| BseYI | CCCAGC | 1 cut(s) | 2262 |
| BsgI | GTGCAG | 2 cut(s) | 441, 952 |
| Bsh1236I | CGCG | 1 cut(s) | 659 |
| Bsh1285I | CGRYCG | 1 cut(s) | 136 |
| BshFI | GGCC | 3 cut(s) | 279, 2238, 2743 |
| BshNI | GGYRCC | 2 cut(s) | 624, 2844 |
| BsiEI | CGRYCG | 1 cut(s) | 136 |
| BsiHKAI | GWGCWC | 4 cut(s) | 115, 192, 1725, 2046 |
| BsiHKCI | CYCGRG | 1 cut(s) | 2415 |
| BsiSI | CCGG | 2 cut(s) | 404, 1175 |
| BslFI | GGGAC | 2 cut(s) | 1252, 2718 |
| BslI | CCNNNNNNNGG | 7 cut(s) | 927, 1003, 1148, 1879, 1940, 2031, 2443 |
| BsmAI | GTCTC | 6 cut(s) | 413, 853, 1209, 1241, 1750, 2836 |
| BsmBI | CGTCTC | 1 cut(s) | 2836 |
| BsmFI | GGGAC | 2 cut(s) | 1252, 2718 |
| BsmI | GAATGC | 1 cut(s) | 1106 |
| BsnI | GGCC | 3 cut(s) | 279, 2238, 2743 |
| BsoBI | CYCGRG | 1 cut(s) | 2415 |
| Bsp119I | TTCGAA | 2 cut(s) | 51, 233 |
| Bsp120I | GGGCCC | 1 cut(s) | 2236 |
| Bsp1286I | GDGCHC | 6 cut(s) | 115, 192, 1673, 1725, 2046, 2240 |
| Bsp13I | TCCGGA | 1 cut(s) | 1174 |
| Bsp1407I | TGTACA | 1 cut(s) | 2730 |
| Bsp1720I | GCTNAGC | 2 cut(s) | 1809, 2538 |
| Bsp19I | CCATGG | 1 cut(s) | 2618 |
| BspACI | CCGC | 9 cut(s) | 136, 179, 197, 659, 1049, 1739, 1798, 2267, 2566 |
| BspANI | GGCC | 3 cut(s) | 279, 2238, 2743 |
| BspEI | TCCGGA | 1 cut(s) | 1174 |
| BspFNI | CGCG | 1 cut(s) | 659 |
| BspLI | GGNNCC | 7 cut(s) | 626, 1517, 1962, 2237, 2238, 2549, 2846 |
| BspMAI | CTGCAG | 2 cut(s) | 390, 3088 |
| BspMI | ACCTGC | 1 cut(s) | 2989 |
| BspOI | GCTAGC | 3 cut(s) | 179, 221, 657 |
| BspT104I | TTCGAA | 2 cut(s) | 51, 233 |
| BspT107I | GGYRCC | 2 cut(s) | 624, 2844 |
| BspTI | CTTAAG | 1 cut(s) | 3011 |
| BsrDI | GCAATG | 7 cut(s) | 300, 730, 1111, 1239, 1800, 2179, 2393 |
| BsrGI | TGTACA | 1 cut(s) | 2730 |
| BsrI | ACTGG | 8 cut(s) | 641, 748, 957, 1153, 1284, 1707, 1791, 2752 |
| BssECI | CCNNGG | 5 cut(s) | 27, 402, 1269, 2532, 2618 |
| BssNI | GRCGYC | 2 cut(s) | 625, 2845 |
| BssSI | CACGAG | 1 cut(s) | 267 |
| BssT1I | CCWWGG | 1 cut(s) | 2618 |
| Bst2BI | CACGAG | 1 cut(s) | 267 |
| Bst2UI | CCWGG | 7 cut(s) | 86, 1271, 1941, 2271, 2519, 2534, 2679 |
| Bst4CI | ACNGT | 3 cut(s) | 424, 1679, 1978 |
| Bst6I | CTCTTC | 3 cut(s) | 549, 1227, 1546 |
| BstACI | GRCGYC | 2 cut(s) | 625, 2845 |
| BstAFI | CTTAAG | 1 cut(s) | 3011 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 1101, 1907 |
| BstAUI | TGTACA | 1 cut(s) | 2730 |
| BstBI | TTCGAA | 2 cut(s) | 51, 233 |
| BstDSI | CCRYGG | 1 cut(s) | 2618 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 2441 |
| BstFNI | CGCG | 1 cut(s) | 659 |
| BstH2I | RGCGCY | 2 cut(s) | 628, 2848 |
| BstHHI | GCGC | 3 cut(s) | 627, 659, 2847 |
| BstMAI | GTCTC | 6 cut(s) | 413, 853, 1209, 1241, 1750, 2836 |
| BstMCI | CGRYCG | 1 cut(s) | 136 |
| BstMWI | GCNNNNNNNGC | 9 cut(s) | 158, 211, 388, 939, 1101, 1303, 1591, 1859, 1907 |
| BstNI | CCWGG | 7 cut(s) | 86, 1271, 1941, 2271, 2519, 2534, 2679 |
| BstNSI | RCATGY | 3 cut(s) | 1485, 1631, 2172 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 1593 |
| BstSCI | CCNGG | 8 cut(s) | 84, 402, 1269, 1939, 2269, 2517, 2532, 2677 |
| BstSFI | CTRYAG | 4 cut(s) | 139, 386, 2589, 3084 |
| BstSLI | GKGCMC | 2 cut(s) | 1673, 2240 |
| BstUI | CGCG | 1 cut(s) | 659 |
| BstV2I | GAAGAC | 4 cut(s) | 60, 1385, 1393, 2721 |
| BstX2I | RGATCY | 8 cut(s) | 963, 1187, 1322, 1411, 1508, 1515, 2448, 2940 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 1660, 2270 |
| BstYI | RGATCY | 8 cut(s) | 963, 1187, 1322, 1411, 1508, 1515, 2448, 2940 |
| BsuI | GTATCC | 2 cut(s) | 1783, 2081 |
| BsuRI | GGCC | 3 cut(s) | 279, 2238, 2743 |
| BtgI | CCRYGG | 1 cut(s) | 2618 |
| BtgZI | GCGATG | 1 cut(s) | 3042 |
| BtsIMutI | CAGTG | 7 cut(s) | 741, 1019, 1095, 1472, 1684, 2010, 2709 |
| BveI | ACCTGC | 1 cut(s) | 2989 |
| CaiI | CAGNNNCTG | 1 cut(s) | 385 |
| CfoI | GCGC | 3 cut(s) | 627, 659, 2847 |
| Cfr13I | GGNCC | 6 cut(s) | 406, 1172, 1281, 2236, 2237, 2675 |
| CseI | GACGC | 3 cut(s) | 210, 1583, 2387 |
| CsiI | ACCWGGT | 1 cut(s) | 2677 |
| Csp6I | GTAC | 7 cut(s) | 698, 1784, 2035, 2286, 2318, 2731, 3008 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 625, 660 |
| CviQI | GTAC | 7 cut(s) | 698, 1784, 2035, 2286, 2318, 2731, 3008 |
| DinI | GGCGCC | 2 cut(s) | 626, 2846 |
| DraI | TTTAAA | 2 cut(s) | 528, 732 |
| DriI | GACNNNNNGTC | 1 cut(s) | 1742 |
| EaeI | YGGCCR | 1 cut(s) | 277 |
| Eam1104I | CTCTTC | 3 cut(s) | 549, 1227, 1546 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 1742 |
| EarI | CTCTTC | 3 cut(s) | 549, 1227, 1546 |
| EciI | GGCGGA | 1 cut(s) | 212 |
| Ecl136II | GAGCTC | 2 cut(s) | 113, 1723 |
| Eco130I | CCWWGG | 1 cut(s) | 2618 |
| Eco24I | GRGCYC | 3 cut(s) | 115, 1725, 2240 |
| Eco32I | GATATC | 2 cut(s) | 22, 2185 |
| Eco47I | GGWCC | 4 cut(s) | 406, 1172, 1281, 2675 |
| Eco53kI | GAGCTC | 2 cut(s) | 113, 1723 |
| Eco57I | CTGAAG | 3 cut(s) | 327, 573, 2733 |
| Eco88I | CYCGRG | 1 cut(s) | 2415 |
| EcoICRI | GAGCTC | 2 cut(s) | 113, 1723 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 2441 |
| EcoO109I | RGGNCCY | 2 cut(s) | 2236, 2237 |
| EcoRI | GAATTC | 1 cut(s) | 2501 |
| EcoRII | CCWGG | 7 cut(s) | 84, 1269, 1939, 2269, 2517, 2532, 2677 |
| EcoRV | GATATC | 2 cut(s) | 22, 2185 |
| EcoT14I | CCWWGG | 1 cut(s) | 2618 |
| EcoT38I | GRGCYC | 3 cut(s) | 115, 1725, 2240 |
| EgeI | GGCGCC | 2 cut(s) | 626, 2846 |
| EheI | GGCGCC | 2 cut(s) | 626, 2846 |
| ErhI | CCWWGG | 1 cut(s) | 2618 |
| Esp3I | CGTCTC | 1 cut(s) | 2836 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 1356, 1388, 1574, 1606 |
| FaqI | GGGAC | 2 cut(s) | 1252, 2718 |
| FauI | CCCGC | 2 cut(s) | 172, 652 |
| FauNDI | CATATG | 1 cut(s) | 1837 |
| FbaI | TGATCA | 2 cut(s) | 1081, 2007 |
| FblI | GTMKAC | 4 cut(s) | 132, 328, 1624, 2487 |
| FriOI | GRGCYC | 3 cut(s) | 115, 1725, 2240 |
| GlaI | GCGC | 3 cut(s) | 626, 658, 2846 |
| GsaI | CCCAGC | 1 cut(s) | 2266 |
| GsuI | CTGGAG | 2 cut(s) | 107, 227 |
| HaeII | RGCGCY | 2 cut(s) | 628, 2848 |
| HaeIII | GGCC | 3 cut(s) | 279, 2238, 2743 |
| HapII | CCGG | 2 cut(s) | 404, 1175 |
| HgaI | GACGC | 3 cut(s) | 210, 1583, 2387 |
| HhaI | GCGC | 3 cut(s) | 627, 659, 2847 |
| Hin1I | GRCGYC | 2 cut(s) | 625, 2845 |
| Hin6I | GCGC | 3 cut(s) | 625, 657, 2845 |
| HinP1I | GCGC | 3 cut(s) | 625, 657, 2845 |
| HincII | GTYRAC | 1 cut(s) | 133 |
| HindII | GTYRAC | 1 cut(s) | 133 |
| HindIII | AAGCTT | 2 cut(s) | 1295, 1370 |
| HinfI | GANTC | 8 cut(s) | 977, 1178, 1265, 1490, 1616, 1823, 2096, 2635 |
| HpaII | CCGG | 2 cut(s) | 404, 1175 |
| HphI | GGTGA | 5 cut(s) | 580, 812, 1740, 2089, 2867 |
| Hpy166II | GTNNAC | 6 cut(s) | 133, 329, 1625, 2488, 2757, 2912 |
| Hpy8I | GTNNAC | 6 cut(s) | 133, 329, 1625, 2488, 2757, 2912 |
| Hpy99I | CGWCG | 3 cut(s) | 16, 226, 717 |
| HpyAV | CCTTC | 5 cut(s) | 1272, 1804, 1975, 2695, 2782 |
| HpyCH4III | ACNGT | 3 cut(s) | 424, 1679, 1978 |
| HpyCH4IV | ACGT | 2 cut(s) | 712, 2796 |
| HpyF10VI | GCNNNNNNNGC | 9 cut(s) | 158, 211, 388, 939, 1101, 1303, 1591, 1859, 1907 |
| HpySE526I | ACGT | 2 cut(s) | 712, 2796 |
| Hsp92I | GRCGYC | 2 cut(s) | 625, 2845 |
| HspAI | GCGC | 3 cut(s) | 625, 657, 2845 |
| KasI | GGCGCC | 2 cut(s) | 624, 2844 |
| Kpn2I | TCCGGA | 1 cut(s) | 1174 |
| Ksp22I | TGATCA | 2 cut(s) | 1081, 2007 |
| LweI | GCATC | 7 cut(s) | 353, 992, 1633, 1888, 2077, 2098, 3043 |
| MabI | ACCWGGT | 1 cut(s) | 2677 |
| MaeII | ACGT | 2 cut(s) | 712, 2796 |
| MaeIII | GTNAC | 4 cut(s) | 823, 835, 2191, 2432 |
| MfeI | CAATTG | 1 cut(s) | 1329 |
| MflI | RGATCY | 8 cut(s) | 963, 1187, 1322, 1411, 1508, 1515, 2448, 2940 |
| MhlI | GDGCHC | 6 cut(s) | 115, 192, 1673, 1725, 2046, 2240 |
| Mly113I | GGCGCC | 2 cut(s) | 625, 2845 |
| MlyI | GAGTC | 4 cut(s) | 1172, 1274, 1484, 2629 |
| MmeI | TCCRAC | 6 cut(s) | 88, 204, 1082, 1255, 2065, 2653 |
| MroI | TCCGGA | 1 cut(s) | 1174 |
| MroXI | GAANNNNTTC | 1 cut(s) | 2584 |
| MseI | TTAA | 8 cut(s) | 474, 527, 731, 1428, 1577, 1776, 2684, 3012 |
| MslI | CAYNNNNRTG | 3 cut(s) | 2774, 2817, 2853 |
| MspA1I | CMGCKG | 1 cut(s) | 2378 |
| MspCI | CTTAAG | 1 cut(s) | 3011 |
| MspI | CCGG | 2 cut(s) | 404, 1175 |
| MspR9I | CCNGG | 8 cut(s) | 86, 404, 1271, 1941, 2271, 2519, 2534, 2679 |
| MunI | CAATTG | 1 cut(s) | 1329 |
| Mva1269I | GAATGC | 1 cut(s) | 1106 |
| MvaI | CCWGG | 7 cut(s) | 86, 1271, 1941, 2271, 2519, 2534, 2679 |
| MvnI | CGCG | 1 cut(s) | 659 |
| MwoI | GCNNNNNNNGC | 9 cut(s) | 158, 211, 388, 939, 1101, 1303, 1591, 1859, 1907 |
| NarI | GGCGCC | 2 cut(s) | 625, 2845 |
| NciI | CCSGG | 1 cut(s) | 404 |
| NcoI | CCATGG | 1 cut(s) | 2618 |
| NdeI | CATATG | 1 cut(s) | 1837 |
| NheI | GCTAGC | 3 cut(s) | 175, 217, 653 |
| NlaIV | GGNNCC | 7 cut(s) | 626, 1517, 1962, 2237, 2238, 2549, 2846 |
| NmeAIII | GCCGAG | 2 cut(s) | 51, 52 |
| NmuCI | GTSAC | 2 cut(s) | 823, 835 |
| NspI | RCATGY | 3 cut(s) | 1485, 1631, 2172 |
| NspV | TTCGAA | 2 cut(s) | 51, 233 |
| PaeI | GCATGC | 1 cut(s) | 2172 |
| PaeR7I | CTCGAG | 1 cut(s) | 2415 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 24, 230 |
| PctI | GAATGC | 1 cut(s) | 1106 |
| PdmI | GAANNNNTTC | 1 cut(s) | 2584 |
| PfeI | GAWTC | 4 cut(s) | 977, 1616, 1823, 2096 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 1940, 2031 |
| PfoI | TCCNGGA | 2 cut(s) | 84, 2517 |
| PleI | GAGTC | 4 cut(s) | 1172, 1273, 1484, 2629 |
| PluTI | GGCGCC | 2 cut(s) | 628, 2848 |
| PpsI | GAGTC | 4 cut(s) | 1172, 1273, 1484, 2629 |
| PshAI | GACNNNNGTC | 1 cut(s) | 1593 |
| PshBI | ATTAAT | 2 cut(s) | 474, 1577 |
| Psp124BI | GAGCTC | 2 cut(s) | 115, 1725 |
| Psp6I | CCWGG | 7 cut(s) | 84, 1269, 1939, 2269, 2517, 2532, 2677 |
| PspFI | CCCAGC | 1 cut(s) | 2262 |
| PspGI | CCWGG | 7 cut(s) | 84, 1269, 1939, 2269, 2517, 2532, 2677 |
| PspN4I | GGNNCC | 7 cut(s) | 626, 1517, 1962, 2237, 2238, 2549, 2846 |
| PspOMI | GGGCCC | 1 cut(s) | 2236 |
| PspPI | GGNCC | 6 cut(s) | 406, 1172, 1281, 2236, 2237, 2675 |
| PstI | CTGCAG | 2 cut(s) | 390, 3088 |
| PstNI | CAGNNNCTG | 1 cut(s) | 385 |
| PsuI | RGATCY | 8 cut(s) | 963, 1187, 1322, 1411, 1508, 1515, 2448, 2940 |
| PvuII | CAGCTG | 1 cut(s) | 2378 |
| RsaI | GTAC | 7 cut(s) | 699, 1785, 2036, 2287, 2319, 2732, 3009 |
| RsaNI | GTAC | 7 cut(s) | 698, 1784, 2035, 2286, 2318, 2731, 3008 |
| RseI | CAYNNNNRTG | 3 cut(s) | 2774, 2817, 2853 |
| SacI | GAGCTC | 2 cut(s) | 115, 1725 |
| SalI | GTCGAC | 1 cut(s) | 131 |
| SaqAI | TTAA | 8 cut(s) | 474, 527, 731, 1428, 1577, 1776, 2684, 3012 |
| Sau96I | GGNCC | 6 cut(s) | 406, 1172, 1281, 2236, 2237, 2675 |
| ScaI | AGTACT | 1 cut(s) | 1785 |
| SchI | GAGTC | 4 cut(s) | 1172, 1274, 1484, 2629 |
| ScrFI | CCNGG | 8 cut(s) | 86, 404, 1271, 1941, 2271, 2519, 2534, 2679 |
| SduI | GDGCHC | 6 cut(s) | 115, 192, 1673, 1725, 2046, 2240 |
| SexAI | ACCWGGT | 1 cut(s) | 2677 |
| SfaNI | GCATC | 7 cut(s) | 353, 992, 1633, 1888, 2077, 2098, 3043 |
| SfcI | CTRYAG | 4 cut(s) | 139, 386, 2589, 3084 |
| SfoI | GGCGCC | 2 cut(s) | 626, 2846 |
| Sfr274I | CTCGAG | 1 cut(s) | 2415 |
| SfuI | TTCGAA | 2 cut(s) | 51, 233 |
| SinI | GGWCC | 4 cut(s) | 406, 1172, 1281, 2675 |
| SlaI | CTCGAG | 1 cut(s) | 2415 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 2774, 2817, 2853 |
| SmlI | CTYRAG | 5 cut(s) | 1987, 2148, 2415, 2944, 3011 |
| SmoI | CTYRAG | 5 cut(s) | 1987, 2148, 2415, 2944, 3011 |
| SphI | GCATGC | 1 cut(s) | 2172 |
| SsiI | CCGC | 9 cut(s) | 136, 179, 197, 659, 1049, 1739, 1798, 2267, 2566 |
| SspDI | GGCGCC | 2 cut(s) | 624, 2844 |
| SspI | AATATT | 1 cut(s) | 1658 |
| SstI | GAGCTC | 2 cut(s) | 115, 1725 |
| StyD4I | CCNGG | 8 cut(s) | 84, 402, 1269, 1939, 2269, 2517, 2532, 2677 |
| StyI | CCWWGG | 1 cut(s) | 2618 |
| TaaI | ACNGT | 3 cut(s) | 424, 1679, 1978 |
| TaiI | ACGT | 2 cut(s) | 715, 2799 |
| TaqI | TCGA | 9 cut(s) | 51, 132, 233, 441, 889, 1144, 1587, 2416, 2958 |
| TaqII | GACCGA | 1 cut(s) | 549 |
| TatI | WGTACW | 2 cut(s) | 1783, 2730 |
| TauI | GCSGC | 3 cut(s) | 1052, 2269, 2568 |
| TfiI | GAWTC | 4 cut(s) | 977, 1616, 1823, 2096 |
| Tru1I | TTAA | 8 cut(s) | 474, 527, 731, 1428, 1577, 1776, 2684, 3012 |
| Tru9I | TTAA | 8 cut(s) | 474, 527, 731, 1428, 1577, 1776, 2684, 3012 |
| TscAI | CASTG | 7 cut(s) | 748, 1019, 1102, 1479, 1684, 2010, 2716 |
| TseFI | GTSAC | 2 cut(s) | 823, 835 |
| Tsp45I | GTSAC | 2 cut(s) | 823, 835 |
| TspGWI | ACGGA | 3 cut(s) | 20, 29, 2315 |
| TspRI | CASTG | 7 cut(s) | 748, 1019, 1102, 1479, 1684, 2010, 2716 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 1940, 2031 |
| Vha464I | CTTAAG | 1 cut(s) | 3011 |
| VpaK11BI | GGWCC | 4 cut(s) | 406, 1172, 1281, 2675 |
| VspI | ATTAAT | 2 cut(s) | 474, 1577 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 2441 |
| XapI | RAATTY | 5 cut(s) | 908, 1137, 1706, 2327, 2501 |
| XbaI | TCTAGA | 1 cut(s) | 1405 |
| XceI | RCATGY | 3 cut(s) | 1485, 1631, 2172 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 2540 |
| XhoI | CTCGAG | 1 cut(s) | 2415 |
| XmiI | GTMKAC | 4 cut(s) | 132, 328, 1624, 2487 |
| XmnI | GAANNNNTTC | 1 cut(s) | 2584 |
| ZrmI | AGTACT | 1 cut(s) | 1785 |