pycom15g32190

Essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0001161
Physical Location & Seq
Forward (+)
31080352 .. 31082877
2526 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 972 bp
ATGGCGAATTCGTTGTTTCTTCCAAATCACACCTTGGTAGCTCCGCTTCCTCGTACCCTCTCTGCTTCCGTCTCCTCGAAACCCAACAAACCCCAACCACCTCTTCTTCCTTGTCCTCCTTCTCTTCGTCATCTAGGGCTTAGCAGGTTCTCTCTAACCACCACCTCTTCCTCCTCCGTTATTAATCACAGCAGTGGCAGCAGTGGCGGCAGTAGGAGGCGTGTCTCTTCCATCAAATCTCGTCTCACCCCTCAAGCCTCGGTTACCCCATTGAATTCCAGAAAGGAAAATAGTAAGAGCAATGCAGGATCTTCTGAAAGCGGAGCGGATCAAGAGCATTTTCCCCCTTTGAAAACCCTCCTTCAAATTTACAAGGAGGCTATTTTTAACGGAGATGGAAAAACTGTTTCCGAAGTAGAGGCTATTATAGAAGTAATAGAAAACGAGAAGAATGAATTAGTCAAGAAAGTATCTTCTGCATCAGCAGAGATAACATCTGGGAAGGAGAAACTTATCCGCTTGCAAGCAGATTTTGATAATTGTAGAAAAAGATTTGAGAAGGAGAGGCTTACTGTAAGGACTGACGCCCAAGGAGAAGTGATTGAGAGTCTTTTGCCCATGGTTGACAATTTTGAGAGAGCCAAACAGTACCTTAAACCCGAGACTGAAAGGGAAGAAAAGATTGATGCAAGTTACCAAGGTATTTACAAGCAATTCATTGAGATTATGAGGAGCTTGCGTGTAGCTGTCGTTCCAACTGTGGGAAAGCCTTTTGATCCTTCACTGCATGAAGCTATTGCACGGGAAGAGTCTCAAGAGTTCACGGAAGGGATTATAATTCAAGAAATCCGCCGCGGGTTTTTACTCGGAGGTCGGCTTCTGAGACCAGCAATGGTTAAAGTCTCATCAGGGCCTGGCAGTAAGAAATCCCCTGTGGCCACCGGGAAAACAACTGCAGGAGTGGAGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GrpE

Protein Analysis

324

Amino Acids

35.39

Weight (kDa)

9.53

Isoelectric Point (pI)

53.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GrpE PF01025 147 - 302 3.5e-39 GrpE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 836
Acc36I ACCTGC 1 cut(s) 135
AccBSI CCGCTC 1 cut(s) 326
AccII CGCG 1 cut(s) 855
AciI CCGC 8 cut(s) 44, 207, 321, 326, 517, 850, 853, 855
AclWI GGATC 3 cut(s) 316, 336, 770
AcoI YGGCCR 1 cut(s) 936
AcsI RAATTY 3 cut(s) 7, 274, 366
AcyI GRCGYC 1 cut(s) 585
AfaI GTAC 2 cut(s) 55, 650
AfiI CCNNNNNNNGG 1 cut(s) 761
AgsI TTSAA 4 cut(s) 274, 352, 365, 842
AjnI CCWGG 1 cut(s) 913
AjuI GAANNNNNNNTTGG 2 cut(s) 87, 119
AleI CACNNNNGTG 1 cut(s) 192
AluBI AGCT 4 cut(s) 41, 735, 746, 794
AluI AGCT 4 cut(s) 41, 735, 746, 794
Alw26I GTCTC 7 cut(s) 76, 229, 248, 656, 816, 877, 907
AlwI GGATC 3 cut(s) 316, 336, 770
AlwNI CAGNNNCTG 1 cut(s) 914
Ama87I CYCGRG 1 cut(s) 659
AoxI GGCC 2 cut(s) 911, 936
ApeKI GCWGC 1 cut(s) 198
ApoI RAATTY 3 cut(s) 7, 274, 366
AseI ATTAAT 1 cut(s) 183
AspS9I GGNCC 1 cut(s) 911
AsuC2I CCSGG 1 cut(s) 943
AsuHPI GGTGA 1 cut(s) 238
AvaI CYCGRG 1 cut(s) 659
BalI TGGCCA 1 cut(s) 938
BarI GAAGNNNNNNTAC 2 cut(s) 30, 62
BbvI GCAGC 1 cut(s) 210
BccI CCATC 2 cut(s) 239, 389
BciT130I CCWGG 1 cut(s) 915
BcnI CCSGG 1 cut(s) 943
BcoDI GTCTC 7 cut(s) 76, 229, 248, 656, 816, 877, 907
BfaI CTAG 1 cut(s) 134
BfmI CTRYAG 1 cut(s) 954
BfuAI ACCTGC 1 cut(s) 135
BisI GCNGC 3 cut(s) 199, 208, 853
BlpI GCTNAGC 1 cut(s) 140
BlsI GCNGC 3 cut(s) 200, 209, 854
Bme1390I CCNGG 2 cut(s) 915, 943
BmeT110I CYCGRG 1 cut(s) 659
BmgT120I GGNCC 1 cut(s) 911
BmrFI CCNGG 2 cut(s) 915, 943
BmsI GCATC 2 cut(s) 488, 676
Bpu1102I GCTNAGC 1 cut(s) 140
BpuEI CTTGAG 2 cut(s) 237, 798
BpuMI CCSGG 1 cut(s) 943
BsaHI GRCGYC 1 cut(s) 585
BsaI GGTCTC 1 cut(s) 877
BsaJI CCNNGG 6 cut(s) 33, 258, 589, 618, 697, 853
BsaXI ACNNNNNCTCC 2 cut(s) 724, 754
Bsc4I CCNNNNNNNGG 1 cut(s) 761
Bse3DI GCAATG 2 cut(s) 307, 897
BseBI CCWGG 1 cut(s) 915
BseDI CCNNGG 6 cut(s) 33, 258, 589, 618, 697, 853
BseLI CCNNNNNNNGG 1 cut(s) 761
BseMI GCAATG 2 cut(s) 307, 897
BseMII CTCAG 1 cut(s) 872
BseRI GAGGAG 3 cut(s) 64, 163, 745
BseXI GCAGC 1 cut(s) 210
Bsh1236I CGCG 1 cut(s) 855
BshFI GGCC 2 cut(s) 913, 938
BsiHKCI CYCGRG 1 cut(s) 659
BsiSI CCGG 1 cut(s) 942
BslI CCNNNNNNNGG 1 cut(s) 761
BsmAI GTCTC 7 cut(s) 76, 229, 248, 656, 816, 877, 907
BsmBI CGTCTC 2 cut(s) 76, 248
BsnI GGCC 2 cut(s) 913, 938
Bso31I GGTCTC 1 cut(s) 877
BsoBI CYCGRG 1 cut(s) 659
Bsp143I GATC 3 cut(s) 308, 328, 775
Bsp1720I GCTNAGC 1 cut(s) 140
Bsp19I CCATGG 1 cut(s) 618
BspACI CCGC 8 cut(s) 44, 207, 321, 326, 517, 850, 853, 855
BspANI GGCC 2 cut(s) 913, 938
BspCNI CTCAG 1 cut(s) 873
BspFNI CGCG 1 cut(s) 855
BspMAI CTGCAG 1 cut(s) 958
BspMI ACCTGC 1 cut(s) 135
BspPI GGATC 3 cut(s) 316, 336, 770
BspTNI GGTCTC 1 cut(s) 877
BsrBI CCGCTC 1 cut(s) 326
BsrDI GCAATG 2 cut(s) 307, 897
BssECI CCNNGG 6 cut(s) 33, 258, 589, 618, 697, 853
BssMI GATC 3 cut(s) 308, 328, 775
BssNI GRCGYC 1 cut(s) 585
BssT1I CCWWGG 4 cut(s) 33, 589, 618, 697
Bst2UI CCWGG 1 cut(s) 915
Bst4CI ACNGT 4 cut(s) 406, 574, 648, 760
Bst6I CTCTTC 5 cut(s) 108, 129, 172, 232, 801
BstACI GRCGYC 1 cut(s) 585
BstC8I GCNNGC 3 cut(s) 521, 525, 737
BstDEI CTNAG 2 cut(s) 140, 881
BstDSI CCRYGG 2 cut(s) 618, 853
BstEII GGTNACC 1 cut(s) 262
BstFNI CGCG 1 cut(s) 855
BstKTI GATC 3 cut(s) 311, 331, 778
BstMAI GTCTC 7 cut(s) 76, 229, 248, 656, 816, 877, 907
BstMBI GATC 3 cut(s) 308, 328, 775
BstMWI GCNNNNNNNGC 3 cut(s) 198, 204, 207
BstNI CCWGG 1 cut(s) 915
BstPI GGTNACC 1 cut(s) 262
BstSCI CCNGG 2 cut(s) 913, 941
BstSFI CTRYAG 1 cut(s) 954
BstUI CGCG 1 cut(s) 855
BstV1I GCAGC 1 cut(s) 210
BstX2I RGATCY 1 cut(s) 308
BstYI RGATCY 1 cut(s) 308
BsuRI GGCC 2 cut(s) 913, 938
BtgI CCRYGG 2 cut(s) 618, 853
BtsI GCAGTG 3 cut(s) 199, 208, 782
BtsIMutI CAGTG 3 cut(s) 199, 208, 782
BveI ACCTGC 1 cut(s) 135
Cac8I GCNNGC 3 cut(s) 521, 525, 737
CaiI CAGNNNCTG 1 cut(s) 914
Cfr13I GGNCC 1 cut(s) 911
Cfr42I CCGCGG 1 cut(s) 856
CseI GACGC 1 cut(s) 593
Csp6I GTAC 2 cut(s) 54, 649
CviAII CATG 2 cut(s) 619, 788
CviQI GTAC 2 cut(s) 54, 649
DdeI CTNAG 2 cut(s) 140, 881
DpnI GATC 3 cut(s) 310, 330, 777
DpnII GATC 3 cut(s) 308, 328, 775
EaeI YGGCCR 1 cut(s) 936
Eam1104I CTCTTC 5 cut(s) 108, 129, 172, 232, 801
EarI CTCTTC 5 cut(s) 108, 129, 172, 232, 801
EciI GGCGGA 1 cut(s) 839
Eco130I CCWWGG 4 cut(s) 33, 589, 618, 697
Eco31I GGTCTC 1 cut(s) 877
Eco88I CYCGRG 1 cut(s) 659
Eco91I GGTNACC 1 cut(s) 262
EcoO109I RGGNCCY 1 cut(s) 911
EcoO65I GGTNACC 1 cut(s) 262
EcoRI GAATTC 2 cut(s) 7, 274
EcoRII CCWGG 1 cut(s) 913
EcoT14I CCWWGG 4 cut(s) 33, 589, 618, 697
ErhI CCWWGG 4 cut(s) 33, 589, 618, 697
Esp3I CGTCTC 2 cut(s) 76, 248
FaeI CATG 2 cut(s) 622, 791
FaiI YATR 5 cut(s) 428, 620, 728, 789, 836
FatI CATG 2 cut(s) 618, 787
FauI CCCGC 1 cut(s) 848
Fnu4HI GCNGC 3 cut(s) 199, 208, 853
Fsp4HI GCNGC 3 cut(s) 199, 208, 853
FspBI CTAG 1 cut(s) 134
GluI GCNGC 3 cut(s) 199, 208, 853
HaeIII GGCC 2 cut(s) 913, 938
HapII CCGG 1 cut(s) 942
HgaI GACGC 1 cut(s) 593
Hin1I GRCGYC 1 cut(s) 585
Hin1II CATG 2 cut(s) 622, 791
HincII GTYRAC 1 cut(s) 625
HindII GTYRAC 1 cut(s) 625
HinfI GANTC 2 cut(s) 607, 809
HpaII CCGG 1 cut(s) 942
HphI GGTGA 1 cut(s) 238
Hpy166II GTNNAC 2 cut(s) 625, 822
Hpy188I TCNGA 4 cut(s) 316, 412, 869, 882
Hpy188III TCNNGA 5 cut(s) 279, 332, 463, 815, 842
Hpy8I GTNNAC 2 cut(s) 625, 822
HpyAV CCTTC 6 cut(s) 129, 371, 496, 553, 789, 821
HpyCH4III ACNGT 4 cut(s) 406, 574, 648, 760
HpyCH4V TGCA 7 cut(s) 305, 479, 523, 689, 787, 800, 956
HpyF10VI GCNNNNNNNGC 3 cut(s) 198, 204, 207
HpyF3I CTNAG 2 cut(s) 140, 881
Hsp92I GRCGYC 1 cut(s) 585
Hsp92II CATG 2 cut(s) 622, 791
KspI CCGCGG 1 cut(s) 856
Kzo9I GATC 3 cut(s) 308, 328, 775
LmnI GCTCC 3 cut(s) 46, 323, 732
Lsp1109I GCAGC 1 cut(s) 210
LweI GCATC 2 cut(s) 488, 676
MaeI CTAG 1 cut(s) 134
MaeIII GTNAC 2 cut(s) 262, 692
MalI GATC 3 cut(s) 310, 330, 777
MbiI CCGCTC 1 cut(s) 326
MboI GATC 3 cut(s) 308, 328, 775
MflI RGATCY 1 cut(s) 308
MlsI TGGCCA 1 cut(s) 938
MluCI AATT 8 cut(s) 7, 274, 366, 455, 538, 628, 713, 837
MluNI TGGCCA 1 cut(s) 938
MlyI GAGTC 2 cut(s) 616, 818
MmeI TCCRAC 1 cut(s) 779
Mox20I TGGCCA 1 cut(s) 938
MscI TGGCCA 1 cut(s) 938
MseI TTAA 4 cut(s) 183, 387, 654, 897
MslI CAYNNNNRTG 1 cut(s) 192
Msp20I TGGCCA 1 cut(s) 938
MspA1I CMGCKG 1 cut(s) 855
MspI CCGG 1 cut(s) 942
MspR9I CCNGG 2 cut(s) 915, 943
MvaI CCWGG 1 cut(s) 915
MvnI CGCG 1 cut(s) 855
MwoI GCNNNNNNNGC 3 cut(s) 198, 204, 207
NciI CCSGG 1 cut(s) 943
NcoI CCATGG 1 cut(s) 618
NdeII GATC 3 cut(s) 308, 328, 775
NlaIII CATG 2 cut(s) 622, 791
OliI CACNNNNGTG 1 cut(s) 192
PkrI GCNGC 3 cut(s) 200, 209, 854
PleI GAGTC 2 cut(s) 615, 817
PpsI GAGTC 2 cut(s) 615, 817
PshBI ATTAAT 1 cut(s) 183
PsiI TTATAA 1 cut(s) 836
Psp6I CCWGG 1 cut(s) 913
PspEI GGTNACC 1 cut(s) 262
PspGI CCWGG 1 cut(s) 913
PspPI GGNCC 1 cut(s) 911
PsrI GAACNNNNNNTAC 2 cut(s) 735, 767
PstI CTGCAG 1 cut(s) 958
PstNI CAGNNNCTG 1 cut(s) 914
PsuI RGATCY 1 cut(s) 308
RsaI GTAC 2 cut(s) 55, 650
RsaNI GTAC 2 cut(s) 54, 649
RseI CAYNNNNRTG 1 cut(s) 192
SacII CCGCGG 1 cut(s) 856
SaqAI TTAA 4 cut(s) 183, 387, 654, 897
SatI GCNGC 3 cut(s) 199, 208, 853
Sau3AI GATC 3 cut(s) 308, 328, 775
Sau96I GGNCC 1 cut(s) 911
SchI GAGTC 2 cut(s) 616, 818
ScrFI CCNGG 2 cut(s) 915, 943
SfaNI GCATC 2 cut(s) 488, 676
SfcI CTRYAG 1 cut(s) 954
Sfr303I CCGCGG 1 cut(s) 856
SgrBI CCGCGG 1 cut(s) 856
SmiMI CAYNNNNRTG 1 cut(s) 192
SmlI CTYRAG 2 cut(s) 252, 813
SmoI CTYRAG 2 cut(s) 252, 813
Sse9I AATT 8 cut(s) 7, 274, 366, 455, 538, 628, 713, 837
SsiI CCGC 8 cut(s) 44, 207, 321, 326, 517, 850, 853, 855
SspMI CTAG 1 cut(s) 134
StyD4I CCNGG 2 cut(s) 913, 941
StyI CCWWGG 4 cut(s) 33, 589, 618, 697
TaaI ACNGT 4 cut(s) 406, 574, 648, 760
TaqI TCGA 1 cut(s) 77
TasI AATT 8 cut(s) 7, 274, 366, 455, 538, 628, 713, 837
TauI GCSGC 2 cut(s) 210, 855
Tru1I TTAA 4 cut(s) 183, 387, 654, 897
Tru9I TTAA 4 cut(s) 183, 387, 654, 897
TscAI CASTG 3 cut(s) 199, 208, 789
TseI GCWGC 1 cut(s) 198
TspDTI ATGAA 3 cut(s) 468, 706, 804
TspGWI ACGGA 4 cut(s) 58, 166, 405, 839
TspRI CASTG 3 cut(s) 199, 208, 789
VspI ATTAAT 1 cut(s) 183
XapI RAATTY 3 cut(s) 7, 274, 366
XspI CTAG 1 cut(s) 134
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.