pycom16g09290

Vinorine synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0000852
Physical Location & Seq
Forward (+)
6215915 .. 6217213
1299 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1299 bp
ATGAAGATTGAAGTGATTGAGAGAGCTTACATTAAGCCTTCTATGCCAACCCCACCCCATCTTTGTAAGCATGAGTTTTCTTTTCTCGACCAAATCGCAACACAAATTTTCATGCCTATGATCTTTTTCTACAATAATCATCATGTCAACGATGCAAGAACTTATTCAGATAGGGTAAAACAATCTTTATCTGAGGCCTTGACACAATACTATCCGTTGGCTGGACGTATCATTGAAAATGCCTTCATCGATTGCAACGACCAAGGTGCGCCTTATGTCCAAGCCCGAGTGAGGTCCCCACCACTTTCCGATGTCATATCCGAACCAGACCCAAACCAGCTCAACAAACTACTCCCATGTGAACTAGACAATGTCGGTGACTTGGTTTTTGCTATCCAAGTCAACATGTTCGATTGTGGTGGCATGGCTATTGGAATTTGCTTTTCTCACAAAGTTGCCGATGCATTGTCATTCATCACGTTCCTCAACAGTTGGGCGTCGATATCTCGTGGAGATTGCAGCATTAAAAATAGTACAGTCAGTCCTCCAATTTTTGATTTGGCCACGCTCTTTCCACCTAGAAGCATATCAGGATTCAAACCAAGCGTTGAGAAAGCTAGAGAAAAAATCGTCACAAAAAGGTTTGTTTTCAGTGCTTCCACGATTGCTTCTCTTAGGTCAAGATATACAGATAATAAAGCCAATGAGATAAAGCCAAGTAGGATTGAGGCCTTGTCAGCATTTATATGGGCCCGGTTTATTGCCTCCACTCAAGGAAAGCCGAACCCCAACACTCTTTACAGAGTGGCTCACGCCGTTAACTTGCGGACGAGGATGGACCCACCACCGCCTGAATACCATTTCGGGAACGTGAGCCGGTTTGTCATGTCCTCGCCGTCGTTTGACGAGAGAGAGGACACGTGTTACGGGATGGTGCGCCAGATGAGGCAGGCGATAAAGACAATCGACGGTGATTACATGGCCAAAATCAAAGAGGGCAACGAGCACGTGGAGTTTTTGAAGCAGCTAGCGGAGGAGTCTATGAAGCGTGAAGTAGCGTCGTTTAGCTTTAGCAGCTTGTGCAGGTTCCCATATTATGAGACGGACTTCGGGTGGGGCAAGCCTGTGTGGGTCGCGTCGGCCAGCTTTCCATTTAAGAACCTGGTTTGTTTCATTGATGCTGGGTCGAGTGGTGGGATTGAAGCATGGGTGAATTTGAAGGAGGAAGACATGGCTAAGTTCCAACATGATGAGCAGCTGCTGGCATGTACTTATCCAGCACAAGATGCTAAGGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko00941 ko00945 ko01100 ko01110 map00940 map00941 map00945 map01100 map01110
Pfam Domains
Protein Families
Transferase

Protein Analysis

433

Amino Acids

48.8

Weight (kDa)

6.52

Isoelectric Point (pI)

44.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 1 - 419 3.2e-86 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1074
AccII CGCG 1 cut(s) 1136
AciI CCGC 3 cut(s) 826, 848, 1031
AcoI YGGCCR 3 cut(s) 561, 981, 1140
AcsI RAATTY 3 cut(s) 105, 435, 1213
AcvI CACGTG 2 cut(s) 921, 1009
AcyI GRCGYC 1 cut(s) 497
AfaI GTAC 2 cut(s) 535, 1270
AfiI CCNNNNNNNGG 2 cut(s) 221, 291
AflIII ACRYGT 3 cut(s) 405, 918, 920
AgsI TTSAA 6 cut(s) 11, 236, 598, 1021, 1202, 1219
AjnI CCWGG 1 cut(s) 1161
AluBI AGCT 8 cut(s) 26, 340, 617, 1027, 1068, 1077, 1146, 1258
AluI AGCT 8 cut(s) 26, 340, 617, 1027, 1068, 1077, 1146, 1258
Alw21I GWGCWC 1 cut(s) 1008
Alw26I GTCTC 1 cut(s) 1094
AlwNI CAGNNNCTG 1 cut(s) 1261
Ama87I CYCGRG 1 cut(s) 285
AoxI GGCC 6 cut(s) 195, 561, 729, 750, 981, 1140
ApaI GGGCCC 1 cut(s) 754
ApeKI GCWGC 5 cut(s) 519, 1024, 1074, 1255, 1258
ApoI RAATTY 3 cut(s) 105, 435, 1213
ArsI GACNNNNNNTTYG 2 cut(s) 371, 403
Asp700I GAANNNNTTC 1 cut(s) 163
AspLEI GCGC 2 cut(s) 271, 939
AspS9I GGNCC 4 cut(s) 294, 750, 751, 838
AsuC2I CCSGG 1 cut(s) 754
AsuHPI GGTGA 3 cut(s) 389, 983, 1222
AsuNHI GCTAGC 1 cut(s) 1027
AvaI CYCGRG 1 cut(s) 285
AvaII GGWCC 2 cut(s) 294, 838
BaeGI GKGCMC 1 cut(s) 754
BalI TGGCCA 2 cut(s) 563, 983
BanII GRGCYC 1 cut(s) 754
BauI CACGAG 1 cut(s) 507
BbrPI CACGTG 2 cut(s) 921, 1009
BbsI GAAGAC 1 cut(s) 1233
Bbv12I GWGCWC 1 cut(s) 1008
BbvI GCAGC 5 cut(s) 531, 1036, 1086, 1245, 1267
BccI CCATC 3 cut(s) 66, 829, 925
BceAI ACGGC 2 cut(s) 800, 880
BcgI CGANNNNNNTGC 2 cut(s) 248, 282
BciT130I CCWGG 1 cut(s) 1163
BcnI CCSGG 1 cut(s) 754
BcoDI GTCTC 1 cut(s) 1094
BfaI CTAG 4 cut(s) 365, 579, 618, 1028
BfuAI ACCTGC 1 cut(s) 1074
BisI GCNGC 5 cut(s) 520, 1025, 1075, 1256, 1259
BlsI GCNGC 5 cut(s) 521, 1026, 1076, 1257, 1260
Bme1390I CCNGG 2 cut(s) 754, 1163
Bme18I GGWCC 2 cut(s) 294, 838
BmeT110I CYCGRG 1 cut(s) 285
BmgT120I GGNCC 4 cut(s) 294, 750, 751, 838
BmiI GGNNCC 4 cut(s) 296, 752, 840, 1088
BmrFI CCNGG 2 cut(s) 754, 1163
BmsI GCATC 4 cut(s) 142, 451, 1168, 1276
BmtI GCTAGC 1 cut(s) 1031
BpiI GAAGAC 1 cut(s) 1233
Bpu10I CCTNAGC 1 cut(s) 1290
BpuEI CTTGAG 1 cut(s) 756
BpuMI CCSGG 1 cut(s) 754
Bsa29I ATCGAT 1 cut(s) 249
BsaAI YACGTR 2 cut(s) 921, 1009
BsaHI GRCGYC 1 cut(s) 497
BsaJI CCNNGG 1 cut(s) 262
Bsc4I CCNNNNNNNGG 2 cut(s) 221, 291
Bse118I RCCGGY 1 cut(s) 876
BseBI CCWGG 1 cut(s) 1163
BseCI ATCGAT 1 cut(s) 249
BseDI CCNNGG 1 cut(s) 262
BseGI GGATG 2 cut(s) 840, 936
BseLI CCNNNNNNNGG 2 cut(s) 221, 291
BseMII CTCAG 1 cut(s) 183
BseRI GAGGAG 1 cut(s) 1049
BseSI GKGCMC 1 cut(s) 754
BseXI GCAGC 5 cut(s) 531, 1036, 1086, 1245, 1267
BseYI CCCAGC 1 cut(s) 1181
BsgI GTGCAG 1 cut(s) 1102
Bsh1236I CGCG 1 cut(s) 1136
BshFI GGCC 6 cut(s) 197, 563, 731, 752, 983, 1142
BshVI ATCGAT 1 cut(s) 249
BsiHKAI GWGCWC 1 cut(s) 1008
BsiHKCI CYCGRG 1 cut(s) 285
BsiSI CCGG 2 cut(s) 754, 877
BslFI GGGAC 1 cut(s) 280
BslI CCNNNNNNNGG 2 cut(s) 221, 291
BsmAI GTCTC 1 cut(s) 1094
BsmBI CGTCTC 1 cut(s) 1094
BsmFI GGGAC 1 cut(s) 280
BsnI GGCC 6 cut(s) 197, 563, 731, 752, 983, 1142
BsoBI CYCGRG 1 cut(s) 285
Bsp120I GGGCCC 1 cut(s) 750
Bsp1286I GDGCHC 2 cut(s) 754, 1008
Bsp143I GATC 1 cut(s) 120
BspACI CCGC 3 cut(s) 826, 848, 1031
BspANI GGCC 6 cut(s) 197, 563, 731, 752, 983, 1142
BspCNI CTCAG 1 cut(s) 184
BspDI ATCGAT 1 cut(s) 249
BspFNI CGCG 1 cut(s) 1136
BspLI GGNNCC 4 cut(s) 296, 752, 840, 1088
BspMI ACCTGC 1 cut(s) 1074
BspOI GCTAGC 1 cut(s) 1031
BsrFI RCCGGY 1 cut(s) 876
BssAI RCCGGY 1 cut(s) 876
BssECI CCNNGG 1 cut(s) 262
BssMI GATC 1 cut(s) 120
BssNI GRCGYC 1 cut(s) 497
BssSI CACGAG 1 cut(s) 507
BssT1I CCWWGG 1 cut(s) 262
Bst2BI CACGAG 1 cut(s) 507
Bst2UI CCWGG 1 cut(s) 1163
Bst4CI ACNGT 3 cut(s) 491, 538, 971
BstACI GRCGYC 1 cut(s) 497
BstAPI GCANNNNNTGC 2 cut(s) 1080, 1286
BstBAI YACGTR 2 cut(s) 921, 1009
BstC8I GCNNGC 5 cut(s) 951, 1029, 1121, 1144, 1263
BstDEI CTNAG 4 cut(s) 192, 674, 1236, 1290
BstF5I GGATG 2 cut(s) 840, 936
BstFNI CGCG 1 cut(s) 1136
BstHHI GCGC 2 cut(s) 271, 939
BstKTI GATC 1 cut(s) 123
BstMAI GTCTC 1 cut(s) 1094
BstMBI GATC 1 cut(s) 120
BstMWI GCNNNNNNNGC 5 cut(s) 43, 737, 1074, 1080, 1286
BstNI CCWGG 1 cut(s) 1163
BstNSI RCATGY 2 cut(s) 409, 1269
BstSCI CCNGG 2 cut(s) 752, 1161
BstSLI GKGCMC 1 cut(s) 754
BstUI CGCG 1 cut(s) 1136
BstV1I GCAGC 5 cut(s) 531, 1036, 1086, 1245, 1267
BstV2I GAAGAC 1 cut(s) 1233
Bsu15I ATCGAT 1 cut(s) 249
BsuRI GGCC 6 cut(s) 197, 563, 731, 752, 983, 1142
BsuTUI ATCGAT 1 cut(s) 249
BtsCI GGATG 2 cut(s) 840, 936
BtsIMutI CAGTG 1 cut(s) 658
BveI ACCTGC 1 cut(s) 1074
Cac8I GCNNGC 5 cut(s) 951, 1029, 1121, 1144, 1263
CaiI CAGNNNCTG 1 cut(s) 1261
CfoI GCGC 2 cut(s) 271, 939
Cfr10I RCCGGY 1 cut(s) 876
Cfr13I GGNCC 4 cut(s) 294, 750, 751, 838
ClaI ATCGAT 1 cut(s) 249
CseI GACGC 3 cut(s) 486, 1047, 1125
CsiI ACCWGGT 1 cut(s) 1161
Csp6I GTAC 2 cut(s) 534, 1269
CviQI GTAC 2 cut(s) 534, 1269
DdeI CTNAG 4 cut(s) 192, 674, 1236, 1290
DpnI GATC 1 cut(s) 122
DpnII GATC 1 cut(s) 120
EaeI YGGCCR 3 cut(s) 561, 981, 1140
Eco130I CCWWGG 1 cut(s) 262
Eco147I AGGCCT 2 cut(s) 197, 731
Eco24I GRGCYC 1 cut(s) 754
Eco32I GATATC 1 cut(s) 504
Eco47I GGWCC 2 cut(s) 294, 838
Eco72I CACGTG 2 cut(s) 921, 1009
Eco88I CYCGRG 1 cut(s) 285
EcoO109I RGGNCCY 1 cut(s) 294
EcoRII CCWGG 1 cut(s) 1161
EcoRV GATATC 1 cut(s) 504
EcoT14I CCWWGG 1 cut(s) 262
EcoT22I ATGCAT 1 cut(s) 466
EcoT38I GRGCYC 1 cut(s) 754
ErhI CCWWGG 1 cut(s) 262
Esp3I CGTCTC 1 cut(s) 1094
FaqI GGGAC 1 cut(s) 280
Fnu4HI GCNGC 5 cut(s) 520, 1025, 1075, 1256, 1259
FokI GGATG 2 cut(s) 847, 943
FriOI GRGCYC 1 cut(s) 754
Fsp4HI GCNGC 5 cut(s) 520, 1025, 1075, 1256, 1259
FspBI CTAG 4 cut(s) 365, 579, 618, 1028
GlaI GCGC 2 cut(s) 270, 938
GluI GCNGC 5 cut(s) 520, 1025, 1075, 1256, 1259
GsaI CCCAGC 1 cut(s) 1185
HaeIII GGCC 6 cut(s) 197, 563, 731, 752, 983, 1142
HapII CCGG 2 cut(s) 754, 877
HgaI GACGC 3 cut(s) 486, 1047, 1125
HhaI GCGC 2 cut(s) 271, 939
Hin1I GRCGYC 1 cut(s) 497
Hin6I GCGC 2 cut(s) 269, 937
HinP1I GCGC 2 cut(s) 269, 937
HincII GTYRAC 3 cut(s) 148, 403, 820
HindII GTYRAC 3 cut(s) 148, 403, 820
HinfI GANTC 2 cut(s) 594, 1037
HpaI GTTAAC 1 cut(s) 820
HpaII CCGG 2 cut(s) 754, 877
HphI GGTGA 3 cut(s) 389, 983, 1222
Hpy166II GTNNAC 4 cut(s) 148, 362, 403, 820
Hpy188I TCNGA 4 cut(s) 169, 193, 310, 322
Hpy188III TCNNGA 4 cut(s) 86, 591, 681, 865
Hpy8I GTNNAC 4 cut(s) 148, 362, 403, 820
Hpy99I CGWCG 5 cut(s) 502, 901, 971, 1063, 1141
HpyAV CCTTC 3 cut(s) 48, 253, 1213
HpyCH4III ACNGT 3 cut(s) 491, 538, 971
HpyCH4IV ACGT 5 cut(s) 226, 479, 870, 920, 1008
HpyCH4V TGCA 5 cut(s) 155, 255, 464, 519, 1083
HpyF10VI GCNNNNNNNGC 5 cut(s) 43, 737, 1074, 1080, 1286
HpyF3I CTNAG 4 cut(s) 192, 674, 1236, 1290
HpySE526I ACGT 5 cut(s) 226, 479, 870, 920, 1008
Hsp92I GRCGYC 1 cut(s) 497
HspAI GCGC 2 cut(s) 269, 937
KspAI GTTAAC 1 cut(s) 820
Kzo9I GATC 1 cut(s) 120
Lsp1109I GCAGC 5 cut(s) 531, 1036, 1086, 1245, 1267
LweI GCATC 4 cut(s) 142, 451, 1168, 1276
MabI ACCWGGT 1 cut(s) 1161
MaeI CTAG 4 cut(s) 365, 579, 618, 1028
MaeII ACGT 5 cut(s) 226, 479, 870, 920, 1008
MaeIII GTNAC 3 cut(s) 377, 631, 923
MalI GATC 1 cut(s) 122
MboI GATC 1 cut(s) 120
MboII GAAGA 2 cut(s) 16, 1238
MhlI GDGCHC 2 cut(s) 754, 1008
MlsI TGGCCA 2 cut(s) 563, 983
MluCI AATT 4 cut(s) 105, 435, 549, 1213
MluNI TGGCCA 2 cut(s) 563, 983
MlyI GAGTC 1 cut(s) 1046
MmeI TCCRAC 1 cut(s) 1267
Mox20I TGGCCA 2 cut(s) 563, 983
Mph1103I ATGCAT 1 cut(s) 466
MroXI GAANNNNTTC 1 cut(s) 163
MscI TGGCCA 2 cut(s) 563, 983
MseI TTAA 4 cut(s) 33, 525, 819, 1155
MslI CAYNNNNRTG 2 cut(s) 116, 745
Msp20I TGGCCA 2 cut(s) 563, 983
MspA1I CMGCKG 1 cut(s) 1258
MspI CCGG 2 cut(s) 754, 877
MspR9I CCNGG 2 cut(s) 754, 1163
MvaI CCWGG 1 cut(s) 1163
MvnI CGCG 1 cut(s) 1136
MwoI GCNNNNNNNGC 5 cut(s) 43, 737, 1074, 1080, 1286
NciI CCSGG 1 cut(s) 754
NdeII GATC 1 cut(s) 120
NheI GCTAGC 1 cut(s) 1027
NlaIV GGNNCC 4 cut(s) 296, 752, 840, 1088
NmuCI GTSAC 2 cut(s) 377, 631
NsiI ATGCAT 1 cut(s) 466
NspI RCATGY 2 cut(s) 409, 1269
PceI AGGCCT 2 cut(s) 197, 731
PciI ACATGT 1 cut(s) 405
PcsI WCGNNNNNNNCGW 1 cut(s) 255
PdmI GAANNNNTTC 1 cut(s) 163
PfeI GAWTC 1 cut(s) 594
PflFI GACNNNGTC 1 cut(s) 371
PkrI GCNGC 5 cut(s) 521, 1026, 1076, 1257, 1260
PleI GAGTC 1 cut(s) 1045
PmaCI CACGTG 2 cut(s) 921, 1009
PmlI CACGTG 2 cut(s) 921, 1009
PpsI GAGTC 1 cut(s) 1045
Ppu21I YACGTR 2 cut(s) 921, 1009
PpuMI RGGWCCY 1 cut(s) 294
PscI ACATGT 1 cut(s) 405
Psp5II RGGWCCY 1 cut(s) 294
Psp6I CCWGG 1 cut(s) 1161
PspCI CACGTG 2 cut(s) 921, 1009
PspFI CCCAGC 1 cut(s) 1181
PspGI CCWGG 1 cut(s) 1161
PspN4I GGNNCC 4 cut(s) 296, 752, 840, 1088
PspOMI GGGCCC 1 cut(s) 750
PspPI GGNCC 4 cut(s) 294, 750, 751, 838
PspPPI RGGWCCY 1 cut(s) 294
PstNI CAGNNNCTG 1 cut(s) 1261
PsyI GACNNNGTC 1 cut(s) 371
PvuII CAGCTG 1 cut(s) 1258
RsaI GTAC 2 cut(s) 535, 1270
RsaNI GTAC 2 cut(s) 534, 1269
RseI CAYNNNNRTG 2 cut(s) 116, 745
SaqAI TTAA 4 cut(s) 33, 525, 819, 1155
SatI GCNGC 5 cut(s) 520, 1025, 1075, 1256, 1259
Sau3AI GATC 1 cut(s) 120
Sau96I GGNCC 4 cut(s) 294, 750, 751, 838
SchI GAGTC 1 cut(s) 1046
ScrFI CCNGG 2 cut(s) 754, 1163
SduI GDGCHC 2 cut(s) 754, 1008
SexAI ACCWGGT 1 cut(s) 1161
SfaNI GCATC 4 cut(s) 142, 451, 1168, 1276
SinI GGWCC 2 cut(s) 294, 838
SmiMI CAYNNNNRTG 2 cut(s) 116, 745
SmlI CTYRAG 1 cut(s) 771
SmoI CTYRAG 1 cut(s) 771
Sse9I AATT 4 cut(s) 105, 435, 549, 1213
SseBI AGGCCT 2 cut(s) 197, 731
SsiI CCGC 3 cut(s) 826, 848, 1031
SspMI CTAG 4 cut(s) 365, 579, 618, 1028
StuI AGGCCT 2 cut(s) 197, 731
StyD4I CCNGG 2 cut(s) 752, 1161
StyI CCWWGG 1 cut(s) 262
TaaI ACNGT 3 cut(s) 491, 538, 971
TaiI ACGT 5 cut(s) 229, 482, 873, 923, 1011
TaqI TCGA 6 cut(s) 87, 249, 411, 500, 966, 1187
TasI AATT 4 cut(s) 105, 435, 549, 1213
TatI WGTACW 2 cut(s) 533, 1268
TfiI GAWTC 1 cut(s) 594
Tru1I TTAA 4 cut(s) 33, 525, 819, 1155
Tru9I TTAA 4 cut(s) 33, 525, 819, 1155
TscAI CASTG 1 cut(s) 658
TseFI GTSAC 2 cut(s) 377, 631
TseI GCWGC 5 cut(s) 519, 1024, 1074, 1255, 1258
Tsp45I GTSAC 2 cut(s) 377, 631
TspDTI ATGAA 6 cut(s) 17, 100, 235, 463, 1058, 1162
TspGWI ACGGA 2 cut(s) 204, 1118
TspRI CASTG 1 cut(s) 658
Tth111I GACNNNGTC 1 cut(s) 371
VpaK11BI GGWCC 2 cut(s) 294, 838
XapI RAATTY 3 cut(s) 105, 435, 1213
XceI RCATGY 2 cut(s) 409, 1269
XmnI GAANNNNTTC 1 cut(s) 163
XspI CTAG 4 cut(s) 365, 579, 618, 1028
Zsp2I ATGCAT 1 cut(s) 466
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.