RchiOBHm_Chr5g0047151

Secoisolariciresinol dehydrogenase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0001933
Physical Location & Seq
Forward (+)
43484302 .. 43485163
862 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ32508

Sequence Viewer

Length: 702 bp
ATGCGTACTACAACCAATATAGGGCTTGAAGGTAAGGTAGCAGTGATCACCGGCGGAGCCAGTGGCATCGGATTATCCACCGCCAGACTCTTCTCCAGACACGGCGCTAAAGTCGTGATCGCCGACGTGCAAGATGACTTGGGCCAATCCGTTTGTAGATATCTGAACCCTTCCTCCACCACCTTCATCCATTGCAACGTGACGAACGAAAAAGACGTCGAAAACGCTGTGAACACAGCCACCGCCAAGTATGGAAAGCTAGACATTATGTTTAACAACGCCGGCTTCAGCGACTTGGCCAAGCCGAACATCCTCGACAACAACAAGGCCGATTTCGAGCAGGTCCTCAGAGTGAAACTGGTTGGTGTCTTCTTGGGGATCAAGCACGCAACTCGGGTGATGATGGGAGCTCGAAAAGGGATTATTATCAACACGGCTAGCGTTTGCTCAGTCATAGGAGGCGCTGCGTCGCATGCGTACACGAGTTCGAAGCATGGAGTCGAGGGGCTCAAGAAGAATACAGCGGGAGTCGGTGGTGTTTACTCCAACCTCAAAGGGGCGGTGCTGAAGGCGAAAGATGTGGCGGAGGCTGTTCTTTACTTGGGAAGTGATGAGTCCAAGTATGTTAGTGGGCATAATTTGATAGTAGACGGAGGCTACACTATTGTCAACTCAAGGTTCTGTATGTTTGAGCAATCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
adh_short_C2

Protein Analysis

233

Amino Acids

24.61

Weight (kDa)

9.11

Isoelectric Point (pI)

16.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short PF00106 12 - 175 3.5e-39 short chain dehydrogenase
KR PF08659 15 - 170 8.9e-10 KR domain
adh_short_C2 PF13561 20 - 175 1.9e-37 Enoyl-(Acyl carrier protein) reductase
adh_short_C2 PF13561 187 - 221 4.5e-08 Enoyl-(Acyl carrier protein) reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 219
Acc36I ACCTGC 1 cut(s) 331
AccI GTMKAC 1 cut(s) 648
AciI CCGC 6 cut(s) 54, 81, 243, 524, 560, 584
AclWI GGATC 1 cut(s) 386
AcoI YGGCCR 1 cut(s) 297
AcuI CTGAAG 2 cut(s) 271, 587
AcyI GRCGYC 1 cut(s) 216
AfaI GTAC 2 cut(s) 7, 479
AfiI CCNNNNNNNGG 2 cut(s) 21, 556
AgsI TTSAA 1 cut(s) 29
AjiI CACGTC 1 cut(s) 127
AloI GAACNNNNNNTCC 2 cut(s) 158, 190
AluBI AGCT 2 cut(s) 259, 410
AluI AGCT 2 cut(s) 259, 410
Alw21I GWGCWC 1 cut(s) 412
AlwI GGATC 1 cut(s) 386
Ama87I CYCGRG 1 cut(s) 393
AoxI GGCC 3 cut(s) 142, 297, 327
ApeKI GCWGC 1 cut(s) 464
ArsI GACNNNNNNTTYG 2 cut(s) 201, 233
AspLEI GCGC 2 cut(s) 107, 464
AspS9I GGNCC 2 cut(s) 142, 343
AsuHPI GGTGA 2 cut(s) 40, 409
AsuII TTCGAA 1 cut(s) 488
AsuNHI GCTAGC 1 cut(s) 437
AvaI CYCGRG 1 cut(s) 393
AvaII GGWCC 1 cut(s) 343
BalI TGGCCA 1 cut(s) 299
BanII GRGCYC 2 cut(s) 412, 510
BauI CACGAG 1 cut(s) 481
BbsI GAAGAC 1 cut(s) 361
Bbv12I GWGCWC 1 cut(s) 412
BbvI GCAGC 1 cut(s) 451
BccI CCATC 1 cut(s) 397
BceAI ACGGC 2 cut(s) 118, 450
BcgI CGANNNNNNTGC 2 cut(s) 374, 408
BclI TGATCA 1 cut(s) 45
BfaI CTAG 2 cut(s) 260, 438
BfoI RGCGCY 2 cut(s) 108, 465
BfuAI ACCTGC 1 cut(s) 331
BisI GCNGC 1 cut(s) 465
BlsI GCNGC 1 cut(s) 466
Bme18I GGWCC 1 cut(s) 343
BmeT110I CYCGRG 1 cut(s) 393
BmgBI CACGTC 1 cut(s) 127
BmgT120I GGNCC 2 cut(s) 142, 343
BmiI GGNNCC 1 cut(s) 58
BmsI GCATC 1 cut(s) 75
BmtI GCTAGC 1 cut(s) 441
BpiI GAAGAC 1 cut(s) 361
BpmI CTGGAG 1 cut(s) 79
Bpu14I TTCGAA 1 cut(s) 488
BpuEI CTTGAG 2 cut(s) 494, 658
BsaBI GATNNNNATC 1 cut(s) 425
BsaHI GRCGYC 1 cut(s) 216
BsaXI ACNNNNNCTCC 2 cut(s) 158, 188
Bsc4I CCNNNNNNNGG 2 cut(s) 21, 556
Bse118I RCCGGY 2 cut(s) 50, 281
Bse1I ACTGG 2 cut(s) 60, 363
Bse3DI GCAATG 1 cut(s) 190
Bse8I GATNNNNATC 1 cut(s) 425
BseGI GGATG 2 cut(s) 186, 309
BseJI GATNNNNATC 1 cut(s) 425
BseLI CCNNNNNNNGG 2 cut(s) 21, 556
BseMI GCAATG 1 cut(s) 190
BseMII CTCAG 2 cut(s) 361, 462
BseNI ACTGG 2 cut(s) 60, 363
BseXI GCAGC 1 cut(s) 451
BshFI GGCC 3 cut(s) 144, 299, 329
BsiHKAI GWGCWC 1 cut(s) 412
BsiHKCI CYCGRG 1 cut(s) 393
BsiSI CCGG 2 cut(s) 51, 282
BslI CCNNNNNNNGG 2 cut(s) 21, 556
BsnI GGCC 3 cut(s) 144, 299, 329
BsoBI CYCGRG 1 cut(s) 393
Bsp119I TTCGAA 1 cut(s) 488
Bsp1286I GDGCHC 2 cut(s) 412, 510
Bsp143I GATC 3 cut(s) 45, 117, 378
BspACI CCGC 6 cut(s) 54, 81, 243, 524, 560, 584
BspANI GGCC 3 cut(s) 144, 299, 329
BspCNI CTCAG 2 cut(s) 360, 461
BspLI GGNNCC 1 cut(s) 58
BspMI ACCTGC 1 cut(s) 331
BspOI GCTAGC 1 cut(s) 441
BspPI GGATC 1 cut(s) 386
BspT104I TTCGAA 1 cut(s) 488
BsrDI GCAATG 1 cut(s) 190
BsrFI RCCGGY 2 cut(s) 50, 281
BsrI ACTGG 2 cut(s) 60, 363
BssAI RCCGGY 2 cut(s) 50, 281
BssMI GATC 3 cut(s) 45, 117, 378
BssNI GRCGYC 1 cut(s) 216
BssSI CACGAG 1 cut(s) 481
Bst2BI CACGAG 1 cut(s) 481
Bst6I CTCTTC 1 cut(s) 95
BstACI GRCGYC 1 cut(s) 216
BstBI TTCGAA 1 cut(s) 488
BstC8I GCNNGC 4 cut(s) 283, 387, 439, 474
BstDEI CTNAG 2 cut(s) 347, 448
BstF5I GGATG 2 cut(s) 186, 309
BstH2I RGCGCY 2 cut(s) 108, 465
BstHHI GCGC 2 cut(s) 107, 464
BstKTI GATC 3 cut(s) 48, 120, 381
BstMBI GATC 3 cut(s) 45, 117, 378
BstMWI GCNNNNNNNGC 1 cut(s) 473
BstNSI RCATGY 1 cut(s) 476
BstV1I GCAGC 1 cut(s) 451
BstV2I GAAGAC 1 cut(s) 361
BsuRI GGCC 3 cut(s) 144, 299, 329
BtrI CACGTC 1 cut(s) 127
BtsCI GGATG 2 cut(s) 186, 309
BtsI GCAGTG 1 cut(s) 48
BtsIMutI CAGTG 2 cut(s) 48, 67
BveI ACCTGC 1 cut(s) 331
Cac8I GCNNGC 4 cut(s) 283, 387, 439, 474
CfoI GCGC 2 cut(s) 107, 464
Cfr10I RCCGGY 2 cut(s) 50, 281
Cfr13I GGNCC 2 cut(s) 142, 343
CseI GACGC 1 cut(s) 456
Csp6I GTAC 2 cut(s) 6, 478
CviAII CATG 2 cut(s) 473, 494
CviQI GTAC 2 cut(s) 6, 478
DdeI CTNAG 2 cut(s) 347, 448
DpnI GATC 3 cut(s) 47, 119, 380
DpnII GATC 3 cut(s) 45, 117, 378
EaeI YGGCCR 1 cut(s) 297
Eam1104I CTCTTC 1 cut(s) 95
EarI CTCTTC 1 cut(s) 95
EciI GGCGGA 2 cut(s) 69, 599
Ecl136II GAGCTC 1 cut(s) 410
Eco24I GRGCYC 2 cut(s) 412, 510
Eco32I GATATC 1 cut(s) 161
Eco47I GGWCC 1 cut(s) 343
Eco53kI GAGCTC 1 cut(s) 410
Eco57I CTGAAG 2 cut(s) 271, 587
Eco88I CYCGRG 1 cut(s) 393
EcoICRI GAGCTC 1 cut(s) 410
EcoO109I RGGNCCY 1 cut(s) 343
EcoRV GATATC 1 cut(s) 161
EcoT38I GRGCYC 2 cut(s) 412, 510
FaeI CATG 2 cut(s) 476, 497
FaiI YATR 9 cut(s) 20, 252, 269, 455, 474, 495, 624, 636, 686
FatI CATG 2 cut(s) 472, 493
FauI CCCGC 1 cut(s) 517
FbaI TGATCA 1 cut(s) 45
FblI GTMKAC 1 cut(s) 648
Fnu4HI GCNGC 1 cut(s) 465
FokI GGATG 2 cut(s) 173, 296
FriOI GRGCYC 2 cut(s) 412, 510
Fsp4HI GCNGC 1 cut(s) 465
FspBI CTAG 2 cut(s) 260, 438
GlaI GCGC 2 cut(s) 106, 463
GluI GCNGC 1 cut(s) 465
GsuI CTGGAG 1 cut(s) 79
HaeII RGCGCY 2 cut(s) 108, 465
HaeIII GGCC 3 cut(s) 144, 299, 329
HapII CCGG 2 cut(s) 51, 282
HgaI GACGC 1 cut(s) 456
HhaI GCGC 2 cut(s) 107, 464
Hin1I GRCGYC 1 cut(s) 216
Hin1II CATG 2 cut(s) 476, 497
Hin6I GCGC 2 cut(s) 105, 462
HinP1I GCGC 2 cut(s) 105, 462
HincII GTYRAC 1 cut(s) 670
HindII GTYRAC 1 cut(s) 670
HinfI GANTC 4 cut(s) 87, 498, 528, 614
HpaII CCGG 2 cut(s) 51, 282
HphI GGTGA 2 cut(s) 40, 409
Hpy166II GTNNAC 5 cut(s) 232, 480, 541, 649, 670
Hpy188I TCNGA 3 cut(s) 71, 165, 350
Hpy188III TCNNGA 3 cut(s) 96, 115, 511
Hpy8I GTNNAC 5 cut(s) 232, 480, 541, 649, 670
Hpy99I CGWCG 3 cut(s) 128, 221, 472
HpyAV CCTTC 4 cut(s) 23, 180, 193, 562
HpyCH4IV ACGT 3 cut(s) 126, 198, 216
HpyCH4V TGCA 2 cut(s) 130, 195
HpyF10VI GCNNNNNNNGC 1 cut(s) 473
HpyF3I CTNAG 2 cut(s) 347, 448
HpySE526I ACGT 3 cut(s) 126, 198, 216
Hsp92I GRCGYC 1 cut(s) 216
Hsp92II CATG 2 cut(s) 476, 497
HspAI GCGC 2 cut(s) 105, 462
KroI GCCGGC 1 cut(s) 281
KroNI GCCGGC 1 cut(s) 283
Ksp22I TGATCA 1 cut(s) 45
Kzo9I GATC 3 cut(s) 45, 117, 378
LmnI GCTCC 2 cut(s) 56, 407
LpnPI CCDG 7 cut(s) 64, 73, 97, 109, 295, 326, 344
Lsp1109I GCAGC 1 cut(s) 451
LweI GCATC 1 cut(s) 75
MaeI CTAG 2 cut(s) 260, 438
MaeII ACGT 3 cut(s) 126, 198, 216
MaeIII GTNAC 1 cut(s) 199
MalI GATC 3 cut(s) 47, 119, 380
MboI GATC 3 cut(s) 45, 117, 378
MboII GAAGA 3 cut(s) 82, 361, 526
MhlI GDGCHC 2 cut(s) 412, 510
MlsI TGGCCA 1 cut(s) 299
MluCI AATT 1 cut(s) 637
MluNI TGGCCA 1 cut(s) 299
MlyI GAGTC 4 cut(s) 81, 507, 537, 623
MmeI TCCRAC 1 cut(s) 570
MnlI CCTC 8 cut(s) 184, 323, 356, 452, 496, 560, 580, 647
Mox20I TGGCCA 1 cut(s) 299
MroNI GCCGGC 1 cut(s) 281
MscI TGGCCA 1 cut(s) 299
MseI TTAA 2 cut(s) 273, 700
Msp20I TGGCCA 1 cut(s) 299
MspA1I CMGCKG 1 cut(s) 524
MspI CCGG 2 cut(s) 51, 282
MwoI GCNNNNNNNGC 1 cut(s) 473
NaeI GCCGGC 1 cut(s) 283
NdeII GATC 3 cut(s) 45, 117, 378
NgoMIV GCCGGC 1 cut(s) 281
NheI GCTAGC 1 cut(s) 437
NlaIII CATG 2 cut(s) 476, 497
NlaIV GGNNCC 1 cut(s) 58
NmuCI GTSAC 1 cut(s) 199
NspI RCATGY 1 cut(s) 476
NspV TTCGAA 1 cut(s) 488
PaeI GCATGC 1 cut(s) 476
PcsI WCGNNNNNNNCGW 3 cut(s) 120, 204, 213
PdiI GCCGGC 1 cut(s) 283
PkrI GCNGC 1 cut(s) 466
PleI GAGTC 4 cut(s) 81, 506, 536, 622
PpsI GAGTC 4 cut(s) 81, 506, 536, 622
PpuMI RGGWCCY 1 cut(s) 343
Psp124BI GAGCTC 1 cut(s) 412
Psp5II RGGWCCY 1 cut(s) 343
PspN4I GGNNCC 1 cut(s) 58
PspPI GGNCC 2 cut(s) 142, 343
PspPPI RGGWCCY 1 cut(s) 343
RsaI GTAC 2 cut(s) 7, 479
RsaNI GTAC 2 cut(s) 6, 478
SacI GAGCTC 1 cut(s) 412
SaqAI TTAA 2 cut(s) 273, 700
SatI GCNGC 1 cut(s) 465
Sau3AI GATC 3 cut(s) 45, 117, 378
Sau96I GGNCC 2 cut(s) 142, 343
SchI GAGTC 4 cut(s) 81, 507, 537, 623
SduI GDGCHC 2 cut(s) 412, 510
SfaNI GCATC 1 cut(s) 75
SfuI TTCGAA 1 cut(s) 488
SgrAI CRCCGGYG 1 cut(s) 50
SinI GGWCC 1 cut(s) 343
SmlI CTYRAG 2 cut(s) 509, 673
SmoI CTYRAG 2 cut(s) 509, 673
SphI GCATGC 1 cut(s) 476
Sse9I AATT 1 cut(s) 637
SsiI CCGC 6 cut(s) 54, 81, 243, 524, 560, 584
SspMI CTAG 2 cut(s) 260, 438
SstI GAGCTC 1 cut(s) 412
TaiI ACGT 3 cut(s) 129, 201, 219
TaqI TCGA 6 cut(s) 219, 315, 336, 412, 488, 501
TasI AATT 1 cut(s) 637
Tru1I TTAA 2 cut(s) 273, 700
Tru9I TTAA 2 cut(s) 273, 700
TscAI CASTG 2 cut(s) 48, 67
TseFI GTSAC 1 cut(s) 199
TseI GCWGC 1 cut(s) 464
Tsp45I GTSAC 1 cut(s) 199
TspDTI ATGAA 1 cut(s) 175
TspGWI ACGGA 2 cut(s) 139, 666
TspRI CASTG 2 cut(s) 48, 67
VpaK11BI GGWCC 1 cut(s) 343
XceI RCATGY 1 cut(s) 476
XmiI GTMKAC 1 cut(s) 648
XspI CTAG 2 cut(s) 260, 438
ZraI GACGTC 1 cut(s) 217
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.