RLG00000034472

Secoisolariciresinol dehydrogenase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0001933
Physical Location & Seq
Forward (+)
43355544 .. 43357981
2438 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000034472

Sequence Viewer

Length: 813 bp
ATGGCGAGTTTCCCGTTACTCTCGGCTACTGCAAGAAGGCTTCAAGGGAAGGTAGCAGTGATCACCGGTGGAGCCAGTGGCATTGGATTATCCACCGCCAAACTCTTCTCCAGACACTGCGCTAAAGTCGTGATTGCCGACGTGCAAGATGACTTGGGCCAATCTGTTTGTAGAGATCTAAACCCTTCCTCCACCACCTTCATCCATTGTGATGTGACAAACGAAAAAGACATCGAAAACGCCGTGAACACAGCCACCACCAAGTATGGAAAGCTAGACATTATGTTCAACAACGCCGGCATCAGCGACGCGGCCAAGCCGAATATCCTTGACAACAACCAGGCTGATTTCGAGCAGGTCCTCAGAGTGAACCTGGTTGGTGTCTTCCTGGGAACCAAGCATGCAGCTCGGGTGATGATGGGAGCTCGAAAAGGGACTATTATCAACATGGCTAGCGTTTGCTCGATCATAGGGGGTGGTGCGTCGCATGCGTACACTAGTTCGAAGCATGGAGTCGAGGGGCTCACGAAGAACACGGCGATAGAGCTCGGACAGTACGGAATACGTGTAAATTGTGTGTCGCCATATCTGGTGGAGACGCCGTTGACCAAGGAATTTTTTAAGTTCGACAATAAGGGAGTCGGTGGTGTTTACTCCAACCTCAAAGGGGCGGTGCTGAAGCCGGAAGATGTGGCAGAGGCTGTTCTTTACTTGGGAAGTGATGAGTCCAAGTATGTTAGTGGGCATAATTTGGTAATAGACGGAGGCTACACTATTGTCAACTCAAGGTTCTGTATGTTTGAGCAAACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
adh_short_C2

Protein Analysis

271

Amino Acids

28.78

Weight (kDa)

7.62

Isoelectric Point (pI)

25.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SDR PF23441 12 - 257 1.8e-08 SDR-like rossmann domain
adh_short PF00106 17 - 208 7.3e-53 short chain dehydrogenase
KR PF08659 20 - 176 3.6e-08 KR domain
adh_short_C2 PF13561 25 - 258 2.2e-65 Enoyl-(Acyl carrier protein) reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 346
AccII CGCG 1 cut(s) 311
AciI CCGC 3 cut(s) 96, 311, 671
AcoI YGGCCR 1 cut(s) 312
AcsI RAATTY 1 cut(s) 614
AcuI CTGAAG 1 cut(s) 698
AcyI GRCGYC 1 cut(s) 599
AfaI GTAC 2 cut(s) 494, 557
AfiI CCNNNNNNNGG 1 cut(s) 667
AflIII ACRYGT 1 cut(s) 565
AgeI ACCGGT 1 cut(s) 65
AgsI TTSAA 2 cut(s) 44, 289
AhlI ACTAGT 1 cut(s) 497
AjiI CACGTC 1 cut(s) 142
AjnI CCWGG 3 cut(s) 339, 372, 387
AluBI AGCT 4 cut(s) 274, 407, 425, 547
AluI AGCT 4 cut(s) 274, 407, 425, 547
Alw21I GWGCWC 2 cut(s) 427, 549
Alw26I GTCTC 1 cut(s) 590
AlwNI CAGNNNCTG 2 cut(s) 117, 701
Ama87I CYCGRG 1 cut(s) 408
AoxI GGCC 2 cut(s) 157, 312
ApeKI GCWGC 1 cut(s) 404
ApoI RAATTY 1 cut(s) 614
AsiGI ACCGGT 1 cut(s) 65
AspLEI GCGC 1 cut(s) 122
AspS9I GGNCC 2 cut(s) 157, 358
AsuHPI GGTGA 2 cut(s) 55, 424
AsuII TTCGAA 1 cut(s) 503
AsuNHI GCTAGC 1 cut(s) 452
AvaI CYCGRG 1 cut(s) 408
AvaII GGWCC 1 cut(s) 358
BanII GRGCYC 3 cut(s) 427, 525, 549
BbsI GAAGAC 1 cut(s) 376
Bbv12I GWGCWC 2 cut(s) 427, 549
BbvI GCAGC 1 cut(s) 416
BccI CCATC 1 cut(s) 412
BceAI ACGGC 3 cut(s) 227, 552, 586
BcgI CGANNNNNNTGC 2 cut(s) 389, 423
BciT130I CCWGG 3 cut(s) 341, 374, 389
BclI TGATCA 1 cut(s) 60
BcoDI GTCTC 1 cut(s) 590
BcuI ACTAGT 1 cut(s) 497
BfaI CTAG 3 cut(s) 275, 453, 498
BfuAI ACCTGC 1 cut(s) 346
BglII AGATCT 1 cut(s) 175
BisI GCNGC 2 cut(s) 312, 405
BlsI GCNGC 2 cut(s) 313, 406
Bme1390I CCNGG 3 cut(s) 341, 374, 389
Bme18I GGWCC 1 cut(s) 358
BmeT110I CYCGRG 1 cut(s) 408
BmgBI CACGTC 1 cut(s) 142
BmgT120I GGNCC 2 cut(s) 157, 358
BmiI GGNNCC 2 cut(s) 73, 394
BmrFI CCNGG 3 cut(s) 341, 374, 389
BmsI GCATC 1 cut(s) 309
BmtI GCTAGC 1 cut(s) 456
BpiI GAAGAC 1 cut(s) 376
BpmI CTGGAG 1 cut(s) 94
Bpu14I TTCGAA 1 cut(s) 503
BpuEI CTTGAG 1 cut(s) 769
BsaAI YACGTR 1 cut(s) 566
BsaHI GRCGYC 1 cut(s) 599
BsaJI CCNNGG 2 cut(s) 388, 609
BsaWI WCCGGW 1 cut(s) 65
BsaXI ACNNNNNCTCC 4 cut(s) 173, 203, 587, 617
Bsc4I CCNNNNNNNGG 1 cut(s) 667
Bse118I RCCGGY 2 cut(s) 65, 296
Bse1I ACTGG 1 cut(s) 75
BseBI CCWGG 3 cut(s) 341, 374, 389
BseDI CCNNGG 2 cut(s) 388, 609
BseGI GGATG 1 cut(s) 201
BseLI CCNNNNNNNGG 1 cut(s) 667
BseMII CTCAG 1 cut(s) 376
BseNI ACTGG 1 cut(s) 75
BseXI GCAGC 1 cut(s) 416
Bsh1236I CGCG 1 cut(s) 311
BshFI GGCC 2 cut(s) 159, 314
BshTI ACCGGT 1 cut(s) 65
BsiHKAI GWGCWC 2 cut(s) 427, 549
BsiHKCI CYCGRG 1 cut(s) 408
BsiSI CCGG 3 cut(s) 66, 297, 683
BslFI GGGAC 1 cut(s) 448
BslI CCNNNNNNNGG 1 cut(s) 667
BsmAI GTCTC 1 cut(s) 590
BsmBI CGTCTC 1 cut(s) 590
BsmFI GGGAC 1 cut(s) 448
BsnI GGCC 2 cut(s) 159, 314
BsoBI CYCGRG 1 cut(s) 408
Bsp119I TTCGAA 1 cut(s) 503
Bsp1286I GDGCHC 3 cut(s) 427, 525, 549
Bsp143I GATC 3 cut(s) 60, 175, 465
BspACI CCGC 3 cut(s) 96, 311, 671
BspANI GGCC 2 cut(s) 159, 314
BspCNI CTCAG 1 cut(s) 375
BspFNI CGCG 1 cut(s) 311
BspLI GGNNCC 2 cut(s) 73, 394
BspMI ACCTGC 1 cut(s) 346
BspOI GCTAGC 1 cut(s) 456
BspT104I TTCGAA 1 cut(s) 503
BsrFI RCCGGY 2 cut(s) 65, 296
BsrI ACTGG 1 cut(s) 75
BssAI RCCGGY 2 cut(s) 65, 296
BssECI CCNNGG 2 cut(s) 388, 609
BssMI GATC 3 cut(s) 60, 175, 465
BssNI GRCGYC 1 cut(s) 599
BssT1I CCWWGG 1 cut(s) 609
Bst2UI CCWGG 3 cut(s) 341, 374, 389
Bst4CI ACNGT 1 cut(s) 555
Bst6I CTCTTC 1 cut(s) 110
BstACI GRCGYC 1 cut(s) 599
BstBAI YACGTR 1 cut(s) 566
BstBI TTCGAA 1 cut(s) 503
BstC8I GCNNGC 4 cut(s) 298, 402, 454, 489
BstDEI CTNAG 1 cut(s) 362
BstF5I GGATG 1 cut(s) 201
BstFNI CGCG 1 cut(s) 311
BstHHI GCGC 1 cut(s) 122
BstKTI GATC 3 cut(s) 63, 178, 468
BstMAI GTCTC 1 cut(s) 590
BstMBI GATC 3 cut(s) 60, 175, 465
BstMWI GCNNNNNNNGC 1 cut(s) 488
BstNI CCWGG 3 cut(s) 341, 374, 389
BstNSI RCATGY 2 cut(s) 404, 491
BstSCI CCNGG 3 cut(s) 339, 372, 387
BstUI CGCG 1 cut(s) 311
BstV1I GCAGC 1 cut(s) 416
BstV2I GAAGAC 1 cut(s) 376
BstX2I RGATCY 1 cut(s) 175
BstYI RGATCY 1 cut(s) 175
BsuRI GGCC 2 cut(s) 159, 314
BtrI CACGTC 1 cut(s) 142
BtsCI GGATG 1 cut(s) 201
BtsI GCAGTG 2 cut(s) 63, 115
BtsIMutI CAGTG 3 cut(s) 63, 82, 115
BveI ACCTGC 1 cut(s) 346
Cac8I GCNNGC 4 cut(s) 298, 402, 454, 489
CaiI CAGNNNCTG 2 cut(s) 117, 701
CfoI GCGC 1 cut(s) 122
Cfr10I RCCGGY 2 cut(s) 65, 296
Cfr13I GGNCC 2 cut(s) 157, 358
CseI GACGC 3 cut(s) 317, 471, 607
CsiI ACCWGGT 1 cut(s) 372
Csp6I GTAC 2 cut(s) 493, 556
CspAI ACCGGT 1 cut(s) 65
CviAII CATG 4 cut(s) 401, 448, 488, 509
CviQI GTAC 2 cut(s) 493, 556
DdeI CTNAG 1 cut(s) 362
DpnI GATC 3 cut(s) 62, 177, 467
DpnII GATC 3 cut(s) 60, 175, 465
EaeI YGGCCR 1 cut(s) 312
Eam1104I CTCTTC 1 cut(s) 110
EarI CTCTTC 1 cut(s) 110
Ecl136II GAGCTC 2 cut(s) 425, 547
Eco130I CCWWGG 1 cut(s) 609
Eco24I GRGCYC 3 cut(s) 427, 525, 549
Eco47I GGWCC 1 cut(s) 358
Eco53kI GAGCTC 2 cut(s) 425, 547
Eco57I CTGAAG 1 cut(s) 698
Eco88I CYCGRG 1 cut(s) 408
EcoICRI GAGCTC 2 cut(s) 425, 547
EcoO109I RGGNCCY 1 cut(s) 358
EcoRII CCWGG 3 cut(s) 339, 372, 387
EcoT14I CCWWGG 1 cut(s) 609
EcoT38I GRGCYC 3 cut(s) 427, 525, 549
ErhI CCWWGG 1 cut(s) 609
Esp3I CGTCTC 1 cut(s) 590
FaeI CATG 4 cut(s) 404, 451, 491, 512
FaqI GGGAC 1 cut(s) 448
FatI CATG 4 cut(s) 400, 447, 487, 508
FbaI TGATCA 1 cut(s) 60
Fnu4HI GCNGC 2 cut(s) 312, 405
FokI GGATG 1 cut(s) 188
FriOI GRGCYC 3 cut(s) 427, 525, 549
Fsp4HI GCNGC 2 cut(s) 312, 405
FspBI CTAG 3 cut(s) 275, 453, 498
GlaI GCGC 1 cut(s) 121
GluI GCNGC 2 cut(s) 312, 405
GsuI CTGGAG 1 cut(s) 94
HaeIII GGCC 2 cut(s) 159, 314
HapII CCGG 3 cut(s) 66, 297, 683
HgaI GACGC 3 cut(s) 317, 471, 607
HhaI GCGC 1 cut(s) 122
Hin1I GRCGYC 1 cut(s) 599
Hin1II CATG 4 cut(s) 404, 451, 491, 512
Hin6I GCGC 1 cut(s) 120
HinP1I GCGC 1 cut(s) 120
HincII GTYRAC 2 cut(s) 606, 781
HindII GTYRAC 2 cut(s) 606, 781
HinfI GANTC 3 cut(s) 513, 639, 725
HpaII CCGG 3 cut(s) 66, 297, 683
HphI GGTGA 2 cut(s) 55, 424
Hpy166II GTNNAC 6 cut(s) 247, 370, 495, 606, 652, 781
Hpy188I TCNGA 2 cut(s) 365, 551
Hpy188III TCNNGA 3 cut(s) 111, 130, 526
Hpy8I GTNNAC 6 cut(s) 247, 370, 495, 606, 652, 781
Hpy99I CGWCG 3 cut(s) 143, 311, 487
HpyAV CCTTC 4 cut(s) 30, 43, 195, 208
HpyCH4III ACNGT 1 cut(s) 555
HpyCH4IV ACGT 2 cut(s) 141, 565
HpyCH4V TGCA 3 cut(s) 32, 145, 404
HpyF10VI GCNNNNNNNGC 1 cut(s) 488
HpyF3I CTNAG 1 cut(s) 362
HpySE526I ACGT 2 cut(s) 141, 565
Hsp92I GRCGYC 1 cut(s) 599
Hsp92II CATG 4 cut(s) 404, 451, 491, 512
HspAI GCGC 1 cut(s) 120
KroI GCCGGC 1 cut(s) 296
KroNI GCCGGC 1 cut(s) 298
Ksp22I TGATCA 1 cut(s) 60
Kzo9I GATC 3 cut(s) 60, 175, 465
LmnI GCTCC 2 cut(s) 71, 422
Lsp1109I GCAGC 1 cut(s) 416
LweI GCATC 1 cut(s) 309
MabI ACCWGGT 1 cut(s) 372
MaeI CTAG 3 cut(s) 275, 453, 498
MaeII ACGT 2 cut(s) 141, 565
MaeIII GTNAC 2 cut(s) 15, 214
MalI GATC 3 cut(s) 62, 177, 467
MboI GATC 3 cut(s) 60, 175, 465
MboII GAAGA 4 cut(s) 97, 376, 541, 698
MflI RGATCY 1 cut(s) 175
MhlI GDGCHC 3 cut(s) 427, 525, 549
MluCI AATT 3 cut(s) 571, 614, 748
MlyI GAGTC 3 cut(s) 522, 648, 734
MmeI TCCRAC 1 cut(s) 681
MnlI CCTC 6 cut(s) 199, 371, 511, 671, 691, 758
MroNI GCCGGC 1 cut(s) 296
MseI TTAA 2 cut(s) 621, 811
MslI CAYNNNNRTG 1 cut(s) 210
MspI CCGG 3 cut(s) 66, 297, 683
MspR9I CCNGG 3 cut(s) 341, 374, 389
MvaI CCWGG 3 cut(s) 341, 374, 389
MvnI CGCG 1 cut(s) 311
MwoI GCNNNNNNNGC 1 cut(s) 488
NaeI GCCGGC 1 cut(s) 298
NdeII GATC 3 cut(s) 60, 175, 465
NgoMIV GCCGGC 1 cut(s) 296
NheI GCTAGC 1 cut(s) 452
NlaIII CATG 4 cut(s) 404, 451, 491, 512
NlaIV GGNNCC 2 cut(s) 73, 394
NmuCI GTSAC 1 cut(s) 214
NspI RCATGY 2 cut(s) 404, 491
NspV TTCGAA 1 cut(s) 503
PaeI GCATGC 2 cut(s) 404, 491
PcsI WCGNNNNNNNCGW 2 cut(s) 135, 240
PdiI GCCGGC 1 cut(s) 298
PinAI ACCGGT 1 cut(s) 65
PkrI GCNGC 2 cut(s) 313, 406
PleI GAGTC 3 cut(s) 521, 647, 733
PpsI GAGTC 3 cut(s) 521, 647, 733
Ppu21I YACGTR 1 cut(s) 566
PpuMI RGGWCCY 1 cut(s) 358
Psp124BI GAGCTC 2 cut(s) 427, 549
Psp5II RGGWCCY 1 cut(s) 358
Psp6I CCWGG 3 cut(s) 339, 372, 387
PspGI CCWGG 3 cut(s) 339, 372, 387
PspN4I GGNNCC 2 cut(s) 73, 394
PspPI GGNCC 2 cut(s) 157, 358
PspPPI RGGWCCY 1 cut(s) 358
PstNI CAGNNNCTG 2 cut(s) 117, 701
PsuI RGATCY 1 cut(s) 175
RsaI GTAC 2 cut(s) 494, 557
RsaNI GTAC 2 cut(s) 493, 556
RseI CAYNNNNRTG 1 cut(s) 210
SacI GAGCTC 2 cut(s) 427, 549
SaqAI TTAA 2 cut(s) 621, 811
SatI GCNGC 2 cut(s) 312, 405
Sau3AI GATC 3 cut(s) 60, 175, 465
Sau96I GGNCC 2 cut(s) 157, 358
SchI GAGTC 3 cut(s) 522, 648, 734
ScrFI CCNGG 3 cut(s) 341, 374, 389
SduI GDGCHC 3 cut(s) 427, 525, 549
SexAI ACCWGGT 1 cut(s) 372
SfaNI GCATC 1 cut(s) 309
SfuI TTCGAA 1 cut(s) 503
SgrAI CRCCGGYG 1 cut(s) 65
SinI GGWCC 1 cut(s) 358
SmiMI CAYNNNNRTG 1 cut(s) 210
SmlI CTYRAG 1 cut(s) 784
SmoI CTYRAG 1 cut(s) 784
SpeI ACTAGT 1 cut(s) 497
SphI GCATGC 2 cut(s) 404, 491
Sse9I AATT 3 cut(s) 571, 614, 748
SsiI CCGC 3 cut(s) 96, 311, 671
SspMI CTAG 3 cut(s) 275, 453, 498
SstI GAGCTC 2 cut(s) 427, 549
StyD4I CCNGG 3 cut(s) 339, 372, 387
StyI CCWWGG 1 cut(s) 609
TaaI ACNGT 1 cut(s) 555
TaiI ACGT 2 cut(s) 144, 568
TaqI TCGA 7 cut(s) 234, 351, 427, 464, 503, 516, 627
TasI AATT 3 cut(s) 571, 614, 748
TauI GCSGC 1 cut(s) 314
Tru1I TTAA 2 cut(s) 621, 811
Tru9I TTAA 2 cut(s) 621, 811
TscAI CASTG 3 cut(s) 63, 82, 122
TseFI GTSAC 1 cut(s) 214
TseI GCWGC 1 cut(s) 404
Tsp45I GTSAC 1 cut(s) 214
TspDTI ATGAA 1 cut(s) 190
TspGWI ACGGA 2 cut(s) 573, 777
TspRI CASTG 3 cut(s) 63, 82, 122
VpaK11BI GGWCC 1 cut(s) 358
XapI RAATTY 1 cut(s) 614
XceI RCATGY 2 cut(s) 404, 491
XspI CTAG 3 cut(s) 275, 453, 498
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.