Rh5BG322100

Secoisolariciresinol dehydrogenase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0001933
Physical Location & Seq
Forward (+)
43253991 .. 43311713
57723 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG322100.1

Sequence Viewer

Length: 813 bp
ATGACGAATTTCCTGTTACTCTCAGCTGCTGCAAGAAGGCTTGAAGGTAAGGTAGCAGTGATCACCGGTGGAGCCAGTGGCATCGGAGAATCCACCGCCAGACTCTTCTCCAGACATGGCGCTAAAGTCGTGATTGCCGACGTGCAAGACGACTTGGGCCAATCCGTTTGCAGAGACCTAAACCCTTCGTCCACCACCTTCATCCATTGTGACGTGACAAATGAAAAAGACGTCGAAAATGCTGTGAACACGGCCACCACCAAGTATGGAAAGCTAGACATTATGTTCAACAATGCTGGCATCAGTGGCGTGGTCAACCCAAACATCCTCGTTAACAACAAGGCCGATTTCGAGCAGGTCCTCAAAGTGAACTTGGTTGGTGTCTTCCTGGGGATCAAGCATGCGGCTCGGGTGATGATGGGAGCTAGAAAAGGGACTATTATCAACACTGCTAGCGTTTGCTCGATCATAGGGGGTGGCGCGTCGCATGCGTACACGAGTTCGAAGCATGGAGTCGAGGGGCTCACGAAGAACACAGCGGTGGAGCTCGGAAAGTACGGAATACGTGTGAATTGTGTGTCACCGTATTTGGTGGAGACGTCAATGACTAAGAAATTCTTTAAGTTTGATGATGAAGGAGTGGGTGGTGCTTACTCCAACCTTAATGGGGTGGTGCTGAAGGCGGAAGATGTGGCAGAGGCTGTTCTTTACTTGGGAAGTGATGAGTCCAAGTATGTTAGTGGGCATAATTTGATAGTAGACGGAGGCTACACTATTGTCAACTCAAGGTTCTGTATGTTTGAGCAATCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
adh_short_C2

Protein Analysis

270

Amino Acids

28.67

Weight (kDa)

7.0

Isoelectric Point (pI)

17.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short PF00106 17 - 208 1.3e-53 short chain dehydrogenase
KR PF08659 20 - 176 1.2e-07 KR domain
adh_short_C2 PF13561 25 - 258 9.6e-66 Enoyl-(Acyl carrier protein) reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 234, 602
Acc36I ACCTGC 1 cut(s) 346
AccI GTMKAC 1 cut(s) 759
AccII CGCG 1 cut(s) 482
AciI CCGC 4 cut(s) 96, 404, 539, 683
AclWI GGATC 1 cut(s) 401
AcoI YGGCCR 1 cut(s) 252
AcsI RAATTY 2 cut(s) 7, 614
AcuI CTGAAG 1 cut(s) 698
AcyI GRCGYC 2 cut(s) 231, 599
AfaI GTAC 2 cut(s) 494, 557
AfiI CCNNNNNNNGG 1 cut(s) 667
AflIII ACRYGT 1 cut(s) 565
AgeI ACCGGT 1 cut(s) 65
AgsI TTSAA 2 cut(s) 44, 289
AjiI CACGTC 2 cut(s) 142, 214
AjnI CCWGG 1 cut(s) 387
AleI CACNNNNGTG 1 cut(s) 539
AluBI AGCT 4 cut(s) 26, 274, 425, 547
AluI AGCT 4 cut(s) 26, 274, 425, 547
Alw21I GWGCWC 1 cut(s) 549
Alw26I GTCTC 2 cut(s) 168, 590
AlwI GGATC 1 cut(s) 401
AlwNI CAGNNNCTG 2 cut(s) 29, 701
Ama87I CYCGRG 1 cut(s) 408
AoxI GGCC 3 cut(s) 157, 252, 342
ApeKI GCWGC 2 cut(s) 26, 29
ApoI RAATTY 2 cut(s) 7, 614
AsiGI ACCGGT 1 cut(s) 65
AspLEI GCGC 2 cut(s) 122, 482
AspS9I GGNCC 2 cut(s) 157, 358
AsuHPI GGTGA 3 cut(s) 55, 424, 573
AsuII TTCGAA 1 cut(s) 503
AsuNHI GCTAGC 1 cut(s) 452
AvaI CYCGRG 1 cut(s) 408
AvaII GGWCC 1 cut(s) 358
BanII GRGCYC 2 cut(s) 525, 549
BauI CACGAG 1 cut(s) 496
BbsI GAAGAC 1 cut(s) 376
Bbv12I GWGCWC 1 cut(s) 549
BbvI GCAGC 2 cut(s) 13, 16
BccI CCATC 1 cut(s) 412
BceAI ACGGC 1 cut(s) 267
BcgI CGANNNNNNTGC 2 cut(s) 389, 423
BciT130I CCWGG 1 cut(s) 389
BclI TGATCA 1 cut(s) 60
BcoDI GTCTC 2 cut(s) 168, 590
BfaI CTAG 3 cut(s) 275, 426, 453
BfoI RGCGCY 1 cut(s) 123
BfuAI ACCTGC 1 cut(s) 346
BisI GCNGC 3 cut(s) 27, 30, 405
BlsI GCNGC 3 cut(s) 28, 31, 406
Bme1390I CCNGG 1 cut(s) 389
Bme18I GGWCC 1 cut(s) 358
BmeT110I CYCGRG 1 cut(s) 408
BmgBI CACGTC 2 cut(s) 142, 214
BmgT120I GGNCC 2 cut(s) 157, 358
BmiI GGNNCC 1 cut(s) 73
BmrFI CCNGG 1 cut(s) 389
BmsI GCATC 2 cut(s) 90, 309
BmtI GCTAGC 1 cut(s) 456
BpiI GAAGAC 1 cut(s) 376
BpmI CTGGAG 1 cut(s) 94
Bpu14I TTCGAA 1 cut(s) 503
BpuEI CTTGAG 1 cut(s) 769
BsaAI YACGTR 1 cut(s) 566
BsaHI GRCGYC 2 cut(s) 231, 599
BsaI GGTCTC 1 cut(s) 168
BsaJI CCNNGG 1 cut(s) 388
BsaWI WCCGGW 1 cut(s) 65
Bsc4I CCNNNNNNNGG 1 cut(s) 667
Bse118I RCCGGY 1 cut(s) 65
Bse1I ACTGG 1 cut(s) 75
BseBI CCWGG 1 cut(s) 389
BseDI CCNNGG 1 cut(s) 388
BseGI GGATG 2 cut(s) 201, 324
BseLI CCNNNNNNNGG 1 cut(s) 667
BseMII CTCAG 1 cut(s) 36
BseNI ACTGG 1 cut(s) 75
BseXI GCAGC 2 cut(s) 13, 16
Bsh1236I CGCG 1 cut(s) 482
BshFI GGCC 3 cut(s) 159, 254, 344
BshTI ACCGGT 1 cut(s) 65
BsiHKAI GWGCWC 1 cut(s) 549
BsiHKCI CYCGRG 1 cut(s) 408
BsiSI CCGG 1 cut(s) 66
BslFI GGGAC 1 cut(s) 448
BslI CCNNNNNNNGG 1 cut(s) 667
BsmAI GTCTC 2 cut(s) 168, 590
BsmBI CGTCTC 1 cut(s) 590
BsmFI GGGAC 1 cut(s) 448
BsnI GGCC 3 cut(s) 159, 254, 344
Bso31I GGTCTC 1 cut(s) 168
BsoBI CYCGRG 1 cut(s) 408
Bsp119I TTCGAA 1 cut(s) 503
Bsp1286I GDGCHC 2 cut(s) 525, 549
Bsp143I GATC 3 cut(s) 60, 393, 465
BspACI CCGC 4 cut(s) 96, 404, 539, 683
BspANI GGCC 3 cut(s) 159, 254, 344
BspCNI CTCAG 1 cut(s) 35
BspFNI CGCG 1 cut(s) 482
BspLI GGNNCC 1 cut(s) 73
BspMI ACCTGC 1 cut(s) 346
BspOI GCTAGC 1 cut(s) 456
BspPI GGATC 1 cut(s) 401
BspT104I TTCGAA 1 cut(s) 503
BspTNI GGTCTC 1 cut(s) 168
BsrFI RCCGGY 1 cut(s) 65
BsrI ACTGG 1 cut(s) 75
BssAI RCCGGY 1 cut(s) 65
BssECI CCNNGG 1 cut(s) 388
BssMI GATC 3 cut(s) 60, 393, 465
BssNI GRCGYC 2 cut(s) 231, 599
BssSI CACGAG 1 cut(s) 496
Bst2BI CACGAG 1 cut(s) 496
Bst2UI CCWGG 1 cut(s) 389
Bst4CI ACNGT 1 cut(s) 585
Bst6I CTCTTC 1 cut(s) 110
BstACI GRCGYC 2 cut(s) 231, 599
BstBAI YACGTR 1 cut(s) 566
BstBI TTCGAA 1 cut(s) 503
BstC8I GCNNGC 4 cut(s) 298, 402, 454, 489
BstDEI CTNAG 2 cut(s) 22, 609
BstF5I GGATG 2 cut(s) 201, 324
BstFNI CGCG 1 cut(s) 482
BstH2I RGCGCY 1 cut(s) 123
BstHHI GCGC 2 cut(s) 122, 482
BstKTI GATC 3 cut(s) 63, 396, 468
BstMAI GTCTC 2 cut(s) 168, 590
BstMBI GATC 3 cut(s) 60, 393, 465
BstMWI GCNNNNNNNGC 2 cut(s) 306, 488
BstNI CCWGG 1 cut(s) 389
BstNSI RCATGY 2 cut(s) 404, 491
BstSCI CCNGG 1 cut(s) 387
BstUI CGCG 1 cut(s) 482
BstV1I GCAGC 2 cut(s) 13, 16
BstV2I GAAGAC 1 cut(s) 376
BsuRI GGCC 3 cut(s) 159, 254, 344
BtrI CACGTC 2 cut(s) 142, 214
BtsCI GGATG 2 cut(s) 201, 324
BtsI GCAGTG 2 cut(s) 63, 447
BtsIMutI CAGTG 4 cut(s) 63, 82, 310, 447
BveI ACCTGC 1 cut(s) 346
Cac8I GCNNGC 4 cut(s) 298, 402, 454, 489
CaiI CAGNNNCTG 2 cut(s) 29, 701
CfoI GCGC 2 cut(s) 122, 482
Cfr10I RCCGGY 1 cut(s) 65
Cfr13I GGNCC 2 cut(s) 157, 358
CseI GACGC 1 cut(s) 471
Csp6I GTAC 2 cut(s) 493, 556
CspAI ACCGGT 1 cut(s) 65
CviAII CATG 4 cut(s) 116, 401, 488, 509
CviQI GTAC 2 cut(s) 493, 556
DdeI CTNAG 2 cut(s) 22, 609
DpnI GATC 3 cut(s) 62, 395, 467
DpnII GATC 3 cut(s) 60, 393, 465
EaeI YGGCCR 1 cut(s) 252
Eam1104I CTCTTC 1 cut(s) 110
EarI CTCTTC 1 cut(s) 110
EciI GGCGGA 1 cut(s) 698
Ecl136II GAGCTC 1 cut(s) 547
Eco24I GRGCYC 2 cut(s) 525, 549
Eco31I GGTCTC 1 cut(s) 168
Eco47I GGWCC 1 cut(s) 358
Eco53kI GAGCTC 1 cut(s) 547
Eco57I CTGAAG 1 cut(s) 698
Eco88I CYCGRG 1 cut(s) 408
EcoICRI GAGCTC 1 cut(s) 547
EcoO109I RGGNCCY 1 cut(s) 358
EcoRII CCWGG 1 cut(s) 387
EcoT38I GRGCYC 2 cut(s) 525, 549
Esp3I CGTCTC 1 cut(s) 590
FaeI CATG 4 cut(s) 119, 404, 491, 512
FalI AAGNNNNNCTT 2 cut(s) 602, 634
FaqI GGGAC 1 cut(s) 448
FatI CATG 4 cut(s) 115, 400, 487, 508
FbaI TGATCA 1 cut(s) 60
FblI GTMKAC 1 cut(s) 759
Fnu4HI GCNGC 3 cut(s) 27, 30, 405
FokI GGATG 2 cut(s) 188, 311
FriOI GRGCYC 2 cut(s) 525, 549
Fsp4HI GCNGC 3 cut(s) 27, 30, 405
FspBI CTAG 3 cut(s) 275, 426, 453
GlaI GCGC 2 cut(s) 121, 481
GluI GCNGC 3 cut(s) 27, 30, 405
GsuI CTGGAG 1 cut(s) 94
HaeII RGCGCY 1 cut(s) 123
HaeIII GGCC 3 cut(s) 159, 254, 344
HapII CCGG 1 cut(s) 66
HgaI GACGC 1 cut(s) 471
HhaI GCGC 2 cut(s) 122, 482
Hin1I GRCGYC 2 cut(s) 231, 599
Hin1II CATG 4 cut(s) 119, 404, 491, 512
Hin6I GCGC 2 cut(s) 120, 480
HinP1I GCGC 2 cut(s) 120, 480
HincII GTYRAC 3 cut(s) 316, 334, 781
HindII GTYRAC 3 cut(s) 316, 334, 781
HinfI GANTC 4 cut(s) 89, 102, 513, 725
HpaI GTTAAC 1 cut(s) 334
HpaII CCGG 1 cut(s) 66
HphI GGTGA 3 cut(s) 55, 424, 573
Hpy166II GTNNAC 8 cut(s) 192, 247, 316, 334, 370, 495, 760, 781
Hpy188I TCNGA 2 cut(s) 86, 551
Hpy188III TCNNGA 3 cut(s) 111, 130, 526
Hpy8I GTNNAC 8 cut(s) 192, 247, 316, 334, 370, 495, 760, 781
Hpy99I CGWCG 3 cut(s) 143, 236, 487
HpyAV CCTTC 6 cut(s) 30, 38, 195, 208, 629, 673
HpyCH4III ACNGT 1 cut(s) 585
HpyCH4IV ACGT 5 cut(s) 141, 213, 231, 565, 599
HpyCH4V TGCA 3 cut(s) 32, 145, 171
HpyF10VI GCNNNNNNNGC 2 cut(s) 306, 488
HpyF3I CTNAG 2 cut(s) 22, 609
HpySE526I ACGT 5 cut(s) 141, 213, 231, 565, 599
Hsp92I GRCGYC 2 cut(s) 231, 599
Hsp92II CATG 4 cut(s) 119, 404, 491, 512
HspAI GCGC 2 cut(s) 120, 480
Ksp22I TGATCA 1 cut(s) 60
KspAI GTTAAC 1 cut(s) 334
Kzo9I GATC 3 cut(s) 60, 393, 465
LmnI GCTCC 3 cut(s) 71, 422, 544
LpnPI CCDG 9 cut(s) 26, 79, 88, 112, 124, 282, 341, 374, 401
Lsp1109I GCAGC 2 cut(s) 13, 16
LweI GCATC 2 cut(s) 90, 309
MaeI CTAG 3 cut(s) 275, 426, 453
MaeII ACGT 5 cut(s) 141, 213, 231, 565, 599
MaeIII GTNAC 4 cut(s) 15, 209, 214, 579
MalI GATC 3 cut(s) 62, 395, 467
MboI GATC 3 cut(s) 60, 393, 465
MboII GAAGA 4 cut(s) 97, 376, 541, 698
MhlI GDGCHC 2 cut(s) 525, 549
MluCI AATT 4 cut(s) 7, 571, 614, 748
MlyI GAGTC 3 cut(s) 96, 522, 734
MmeI TCCRAC 1 cut(s) 681
MnlI CCTC 5 cut(s) 338, 371, 511, 691, 758
MseI TTAA 4 cut(s) 333, 621, 663, 811
MslI CAYNNNNRTG 1 cut(s) 539
MspA1I CMGCKG 2 cut(s) 26, 539
MspI CCGG 1 cut(s) 66
MspR9I CCNGG 1 cut(s) 389
MvaI CCWGG 1 cut(s) 389
MvnI CGCG 1 cut(s) 482
MwoI GCNNNNNNNGC 2 cut(s) 306, 488
NdeII GATC 3 cut(s) 60, 393, 465
NheI GCTAGC 1 cut(s) 452
NlaIII CATG 4 cut(s) 119, 404, 491, 512
NlaIV GGNNCC 1 cut(s) 73
NmuCI GTSAC 3 cut(s) 209, 214, 579
NspI RCATGY 2 cut(s) 404, 491
NspV TTCGAA 1 cut(s) 503
OliI CACNNNNGTG 1 cut(s) 539
PaeI GCATGC 2 cut(s) 404, 491
PcsI WCGNNNNNNNCGW 2 cut(s) 135, 147
PfeI GAWTC 1 cut(s) 89
PinAI ACCGGT 1 cut(s) 65
PkrI GCNGC 3 cut(s) 28, 31, 406
PleI GAGTC 3 cut(s) 96, 521, 733
PpsI GAGTC 3 cut(s) 96, 521, 733
Ppu21I YACGTR 1 cut(s) 566
PpuMI RGGWCCY 1 cut(s) 358
Psp124BI GAGCTC 1 cut(s) 549
Psp5II RGGWCCY 1 cut(s) 358
Psp6I CCWGG 1 cut(s) 387
PspGI CCWGG 1 cut(s) 387
PspN4I GGNNCC 1 cut(s) 73
PspPI GGNCC 2 cut(s) 157, 358
PspPPI RGGWCCY 1 cut(s) 358
PstNI CAGNNNCTG 2 cut(s) 29, 701
PvuII CAGCTG 1 cut(s) 26
RsaI GTAC 2 cut(s) 494, 557
RsaNI GTAC 2 cut(s) 493, 556
RseI CAYNNNNRTG 1 cut(s) 539
SacI GAGCTC 1 cut(s) 549
SaqAI TTAA 4 cut(s) 333, 621, 663, 811
SatI GCNGC 3 cut(s) 27, 30, 405
Sau3AI GATC 3 cut(s) 60, 393, 465
Sau96I GGNCC 2 cut(s) 157, 358
SchI GAGTC 3 cut(s) 96, 522, 734
ScrFI CCNGG 1 cut(s) 389
SduI GDGCHC 2 cut(s) 525, 549
SfaNI GCATC 2 cut(s) 90, 309
SfuI TTCGAA 1 cut(s) 503
SgrAI CRCCGGYG 1 cut(s) 65
SinI GGWCC 1 cut(s) 358
SmiMI CAYNNNNRTG 1 cut(s) 539
SmlI CTYRAG 1 cut(s) 784
SmoI CTYRAG 1 cut(s) 784
SphI GCATGC 2 cut(s) 404, 491
Sse9I AATT 4 cut(s) 7, 571, 614, 748
SsiI CCGC 4 cut(s) 96, 404, 539, 683
SspMI CTAG 3 cut(s) 275, 426, 453
SstI GAGCTC 1 cut(s) 549
StyD4I CCNGG 1 cut(s) 387
TaaI ACNGT 1 cut(s) 585
TaiI ACGT 5 cut(s) 144, 216, 234, 568, 602
TaqI TCGA 5 cut(s) 234, 351, 464, 503, 516
TasI AATT 4 cut(s) 7, 571, 614, 748
TauI GCSGC 1 cut(s) 407
TfiI GAWTC 1 cut(s) 89
Tru1I TTAA 4 cut(s) 333, 621, 663, 811
Tru9I TTAA 4 cut(s) 333, 621, 663, 811
TscAI CASTG 4 cut(s) 63, 82, 310, 454
TseFI GTSAC 3 cut(s) 209, 214, 579
TseI GCWGC 2 cut(s) 26, 29
Tsp45I GTSAC 3 cut(s) 209, 214, 579
TspDTI ATGAA 3 cut(s) 190, 237, 648
TspGWI ACGGA 3 cut(s) 154, 573, 777
TspRI CASTG 4 cut(s) 63, 82, 310, 454
VpaK11BI GGWCC 1 cut(s) 358
XapI RAATTY 2 cut(s) 7, 614
XceI RCATGY 2 cut(s) 404, 491
XmiI GTMKAC 1 cut(s) 759
XspI CTAG 3 cut(s) 275, 426, 453
ZraI GACGTC 2 cut(s) 232, 600
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.