Rh5DG333100

Secoisolariciresinol dehydrogenase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0001933
Physical Location & Seq
Forward (+)
44018916 .. 44020122
1207 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG333100.1

Sequence Viewer

Length: 822 bp
ATGGTGCCATTCAGATTAAATTTAACTGTTTGTGGATTGATACGTAGGCTTGAAGGTAAGGTAGCAGTGATCACCGGTGGAGCCAGTGGCATCGGAGAATCCACCGCCAGACTCTTCTCCAGACATGGCGCTAAAGTCGTGATTGCCGACGTGCAAGACGACTTGGGCCAATCCGTTTGCAGAGACCTAAACCCTTCGTCCACCACCTTCATCCATTGTGACGTGACAAATGAAAAAGACGTCGAAAATGCTGTGAACACGGCCACCACCAAGTATGGAAAGCTAGACATTATGTTCAACAATGCTGGCATCAGTGGCGTGGTCAACCCAAACATCCTCGTTAACAACAAGGCCGATTTCGAGCAGGTCCTCAAAGTGAACTTGGTTGGTGTCTTCCTGGGGATCAAGCATGCGGCTCGGGTGATGATGGGAGCTAGAAAAGGGACTATTATCAACACTGCTAGCGTTTGCTCGATCATAGGGGGTGGCGCGTCGCATGCGTACACGAGTTCGAAGCATGGAGTCGAGGGGCTCACGAAGAACACAGCGGTGGAGCTCGGAAAGTACGGAATACGTGTGAATTGTGTGTCACCGTATTTGGTGGAGACGTCAATGACTAAGAAATTCTTTAAGTTTGATGATGAAGGAGTGGGTGGTGCTTACTCCAACCTTAATGGGGTGGTGCTGAAGGCGGAAGATGTGGCAGAGGCTGTTCTATACTTGGGGAGTGATGAGTCCAAGTATGTTAGTGGGCATAATTTGATAGTAGATGGAGGCTACACTATTACCAACTCAGGGTTCTGTATCTTTGAGCAATCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
adh_short_C2

Protein Analysis

273

Amino Acids

28.98

Weight (kDa)

6.99

Isoelectric Point (pI)

18.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short PF00106 20 - 211 1.4e-53 short chain dehydrogenase
KR PF08659 23 - 179 1.2e-07 KR domain
adh_short_C2 PF13561 28 - 261 5.8e-66 Enoyl-(Acyl carrier protein) reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 243, 611
Acc36I ACCTGC 1 cut(s) 355
AccB1I GGYRCC 1 cut(s) 4
AccII CGCG 1 cut(s) 491
AciI CCGC 4 cut(s) 105, 413, 548, 692
AclWI GGATC 1 cut(s) 410
AcoI YGGCCR 1 cut(s) 261
AcsI RAATTY 2 cut(s) 19, 623
AcuI CTGAAG 1 cut(s) 707
AcyI GRCGYC 2 cut(s) 240, 608
AfaI GTAC 2 cut(s) 503, 566
AfiI CCNNNNNNNGG 2 cut(s) 676, 795
AflIII ACRYGT 1 cut(s) 574
AgeI ACCGGT 1 cut(s) 74
AgsI TTSAA 2 cut(s) 53, 298
AjiI CACGTC 2 cut(s) 151, 223
AjnI CCWGG 1 cut(s) 396
AjuI GAANNNNNNNTTGG 2 cut(s) 782, 814
AleI CACNNNNGTG 1 cut(s) 548
AluBI AGCT 3 cut(s) 283, 434, 556
AluI AGCT 3 cut(s) 283, 434, 556
Alw21I GWGCWC 1 cut(s) 558
Alw26I GTCTC 2 cut(s) 177, 599
AlwI GGATC 1 cut(s) 410
AlwNI CAGNNNCTG 1 cut(s) 710
Ama87I CYCGRG 1 cut(s) 417
AoxI GGCC 3 cut(s) 166, 261, 351
ApoI RAATTY 2 cut(s) 19, 623
AsiGI ACCGGT 1 cut(s) 74
AspLEI GCGC 2 cut(s) 131, 491
AspS9I GGNCC 2 cut(s) 166, 367
AsuHPI GGTGA 3 cut(s) 64, 433, 582
AsuII TTCGAA 1 cut(s) 512
AsuNHI GCTAGC 1 cut(s) 461
AvaI CYCGRG 1 cut(s) 417
AvaII GGWCC 1 cut(s) 367
BanI GGYRCC 1 cut(s) 4
BanII GRGCYC 2 cut(s) 534, 558
BauI CACGAG 1 cut(s) 505
BbsI GAAGAC 1 cut(s) 385
Bbv12I GWGCWC 1 cut(s) 558
BccI CCATC 2 cut(s) 421, 764
BceAI ACGGC 1 cut(s) 276
BcgI CGANNNNNNTGC 2 cut(s) 398, 432
BciT130I CCWGG 1 cut(s) 398
BclI TGATCA 1 cut(s) 69
BcoDI GTCTC 2 cut(s) 177, 599
BfaI CTAG 3 cut(s) 284, 435, 462
BfoI RGCGCY 1 cut(s) 132
BfuAI ACCTGC 1 cut(s) 355
BisI GCNGC 1 cut(s) 414
BlsI GCNGC 1 cut(s) 415
Bme1390I CCNGG 1 cut(s) 398
Bme18I GGWCC 1 cut(s) 367
BmeT110I CYCGRG 1 cut(s) 417
BmgBI CACGTC 2 cut(s) 151, 223
BmgT120I GGNCC 2 cut(s) 166, 367
BmiI GGNNCC 2 cut(s) 6, 82
BmrFI CCNGG 1 cut(s) 398
BmsI GCATC 2 cut(s) 99, 318
BmtI GCTAGC 1 cut(s) 465
BpiI GAAGAC 1 cut(s) 385
BpmI CTGGAG 1 cut(s) 103
Bpu14I TTCGAA 1 cut(s) 512
BsaAI YACGTR 2 cut(s) 44, 575
BsaHI GRCGYC 2 cut(s) 240, 608
BsaI GGTCTC 1 cut(s) 177
BsaJI CCNNGG 1 cut(s) 397
BsaWI WCCGGW 1 cut(s) 74
Bsc4I CCNNNNNNNGG 2 cut(s) 676, 795
Bse118I RCCGGY 1 cut(s) 74
Bse1I ACTGG 1 cut(s) 84
BseBI CCWGG 1 cut(s) 398
BseDI CCNNGG 1 cut(s) 397
BseGI GGATG 2 cut(s) 210, 333
BseLI CCNNNNNNNGG 2 cut(s) 676, 795
BseMII CTCAG 1 cut(s) 807
BseNI ACTGG 1 cut(s) 84
Bsh1236I CGCG 1 cut(s) 491
BshFI GGCC 3 cut(s) 168, 263, 353
BshNI GGYRCC 1 cut(s) 4
BshTI ACCGGT 1 cut(s) 74
BsiHKAI GWGCWC 1 cut(s) 558
BsiHKCI CYCGRG 1 cut(s) 417
BsiSI CCGG 1 cut(s) 75
BslFI GGGAC 1 cut(s) 457
BslI CCNNNNNNNGG 2 cut(s) 676, 795
BsmAI GTCTC 2 cut(s) 177, 599
BsmBI CGTCTC 1 cut(s) 599
BsmFI GGGAC 1 cut(s) 457
BsnI GGCC 3 cut(s) 168, 263, 353
Bso31I GGTCTC 1 cut(s) 177
BsoBI CYCGRG 1 cut(s) 417
Bsp119I TTCGAA 1 cut(s) 512
Bsp1286I GDGCHC 2 cut(s) 534, 558
Bsp143I GATC 3 cut(s) 69, 402, 474
BspACI CCGC 4 cut(s) 105, 413, 548, 692
BspANI GGCC 3 cut(s) 168, 263, 353
BspCNI CTCAG 1 cut(s) 806
BspFNI CGCG 1 cut(s) 491
BspLI GGNNCC 2 cut(s) 6, 82
BspMI ACCTGC 1 cut(s) 355
BspOI GCTAGC 1 cut(s) 465
BspPI GGATC 1 cut(s) 410
BspT104I TTCGAA 1 cut(s) 512
BspT107I GGYRCC 1 cut(s) 4
BspTNI GGTCTC 1 cut(s) 177
BsrFI RCCGGY 1 cut(s) 74
BsrI ACTGG 1 cut(s) 84
BssAI RCCGGY 1 cut(s) 74
BssECI CCNNGG 1 cut(s) 397
BssMI GATC 3 cut(s) 69, 402, 474
BssNI GRCGYC 2 cut(s) 240, 608
BssSI CACGAG 1 cut(s) 505
Bst2BI CACGAG 1 cut(s) 505
Bst2UI CCWGG 1 cut(s) 398
Bst4CI ACNGT 2 cut(s) 28, 594
Bst6I CTCTTC 1 cut(s) 119
BstACI GRCGYC 2 cut(s) 240, 608
BstBAI YACGTR 2 cut(s) 44, 575
BstBI TTCGAA 1 cut(s) 512
BstC8I GCNNGC 4 cut(s) 307, 411, 463, 498
BstDEI CTNAG 2 cut(s) 618, 793
BstF5I GGATG 2 cut(s) 210, 333
BstFNI CGCG 1 cut(s) 491
BstH2I RGCGCY 1 cut(s) 132
BstHHI GCGC 2 cut(s) 131, 491
BstKTI GATC 3 cut(s) 72, 405, 477
BstMAI GTCTC 2 cut(s) 177, 599
BstMBI GATC 3 cut(s) 69, 402, 474
BstMWI GCNNNNNNNGC 2 cut(s) 315, 497
BstNI CCWGG 1 cut(s) 398
BstNSI RCATGY 2 cut(s) 413, 500
BstSCI CCNGG 1 cut(s) 396
BstSNI TACGTA 1 cut(s) 44
BstUI CGCG 1 cut(s) 491
BstV2I GAAGAC 1 cut(s) 385
BsuRI GGCC 3 cut(s) 168, 263, 353
BtrI CACGTC 2 cut(s) 151, 223
BtsCI GGATG 2 cut(s) 210, 333
BtsI GCAGTG 2 cut(s) 72, 456
BtsIMutI CAGTG 4 cut(s) 72, 91, 319, 456
BveI ACCTGC 1 cut(s) 355
Cac8I GCNNGC 4 cut(s) 307, 411, 463, 498
CaiI CAGNNNCTG 1 cut(s) 710
CfoI GCGC 2 cut(s) 131, 491
Cfr10I RCCGGY 1 cut(s) 74
Cfr13I GGNCC 2 cut(s) 166, 367
CseI GACGC 1 cut(s) 480
Csp6I GTAC 2 cut(s) 502, 565
CspAI ACCGGT 1 cut(s) 74
CviAII CATG 4 cut(s) 125, 410, 497, 518
CviQI GTAC 2 cut(s) 502, 565
DdeI CTNAG 2 cut(s) 618, 793
DpnI GATC 3 cut(s) 71, 404, 476
DpnII GATC 3 cut(s) 69, 402, 474
EaeI YGGCCR 1 cut(s) 261
Eam1104I CTCTTC 1 cut(s) 119
EarI CTCTTC 1 cut(s) 119
EciI GGCGGA 1 cut(s) 707
Ecl136II GAGCTC 1 cut(s) 556
Eco105I TACGTA 1 cut(s) 44
Eco24I GRGCYC 2 cut(s) 534, 558
Eco31I GGTCTC 1 cut(s) 177
Eco47I GGWCC 1 cut(s) 367
Eco53kI GAGCTC 1 cut(s) 556
Eco57I CTGAAG 1 cut(s) 707
Eco88I CYCGRG 1 cut(s) 417
EcoICRI GAGCTC 1 cut(s) 556
EcoO109I RGGNCCY 1 cut(s) 367
EcoRII CCWGG 1 cut(s) 396
EcoT38I GRGCYC 2 cut(s) 534, 558
Esp3I CGTCTC 1 cut(s) 599
FaeI CATG 4 cut(s) 128, 413, 500, 521
FalI AAGNNNNNCTT 2 cut(s) 611, 643
FaqI GGGAC 1 cut(s) 457
FatI CATG 4 cut(s) 124, 409, 496, 517
FbaI TGATCA 1 cut(s) 69
Fnu4HI GCNGC 1 cut(s) 414
FokI GGATG 2 cut(s) 197, 320
FriOI GRGCYC 2 cut(s) 534, 558
Fsp4HI GCNGC 1 cut(s) 414
FspBI CTAG 3 cut(s) 284, 435, 462
GlaI GCGC 2 cut(s) 130, 490
GluI GCNGC 1 cut(s) 414
GsuI CTGGAG 1 cut(s) 103
HaeII RGCGCY 1 cut(s) 132
HaeIII GGCC 3 cut(s) 168, 263, 353
HapII CCGG 1 cut(s) 75
HgaI GACGC 1 cut(s) 480
HhaI GCGC 2 cut(s) 131, 491
Hin1I GRCGYC 2 cut(s) 240, 608
Hin1II CATG 4 cut(s) 128, 413, 500, 521
Hin6I GCGC 2 cut(s) 129, 489
HinP1I GCGC 2 cut(s) 129, 489
HincII GTYRAC 2 cut(s) 325, 343
HindII GTYRAC 2 cut(s) 325, 343
HinfI GANTC 4 cut(s) 98, 111, 522, 734
HpaI GTTAAC 1 cut(s) 343
HpaII CCGG 1 cut(s) 75
HphI GGTGA 3 cut(s) 64, 433, 582
Hpy166II GTNNAC 6 cut(s) 201, 256, 325, 343, 379, 504
Hpy188I TCNGA 3 cut(s) 14, 95, 560
Hpy188III TCNNGA 3 cut(s) 120, 139, 535
Hpy8I GTNNAC 6 cut(s) 201, 256, 325, 343, 379, 504
Hpy99I CGWCG 3 cut(s) 152, 245, 496
HpyAV CCTTC 5 cut(s) 47, 204, 217, 638, 682
HpyCH4III ACNGT 2 cut(s) 28, 594
HpyCH4IV ACGT 6 cut(s) 43, 150, 222, 240, 574, 608
HpyCH4V TGCA 2 cut(s) 154, 180
HpyF10VI GCNNNNNNNGC 2 cut(s) 315, 497
HpyF3I CTNAG 2 cut(s) 618, 793
HpySE526I ACGT 6 cut(s) 43, 150, 222, 240, 574, 608
Hsp92I GRCGYC 2 cut(s) 240, 608
Hsp92II CATG 4 cut(s) 128, 413, 500, 521
HspAI GCGC 2 cut(s) 129, 489
Ksp22I TGATCA 1 cut(s) 69
KspAI GTTAAC 1 cut(s) 343
Kzo9I GATC 3 cut(s) 69, 402, 474
LmnI GCTCC 3 cut(s) 80, 431, 553
LpnPI CCDG 9 cut(s) 88, 97, 121, 133, 291, 350, 383, 410, 780
LweI GCATC 2 cut(s) 99, 318
MaeI CTAG 3 cut(s) 284, 435, 462
MaeII ACGT 6 cut(s) 43, 150, 222, 240, 574, 608
MaeIII GTNAC 3 cut(s) 218, 223, 588
MalI GATC 3 cut(s) 71, 404, 476
MboI GATC 3 cut(s) 69, 402, 474
MboII GAAGA 4 cut(s) 106, 385, 550, 707
MhlI GDGCHC 2 cut(s) 534, 558
MluCI AATT 4 cut(s) 19, 580, 623, 757
MlyI GAGTC 3 cut(s) 105, 531, 743
MmeI TCCRAC 1 cut(s) 690
MnlI CCTC 5 cut(s) 347, 380, 520, 700, 767
MseI TTAA 6 cut(s) 17, 23, 342, 630, 672, 820
MslI CAYNNNNRTG 1 cut(s) 548
MspA1I CMGCKG 1 cut(s) 548
MspI CCGG 1 cut(s) 75
MspR9I CCNGG 1 cut(s) 398
MvaI CCWGG 1 cut(s) 398
MvnI CGCG 1 cut(s) 491
MwoI GCNNNNNNNGC 2 cut(s) 315, 497
NdeII GATC 3 cut(s) 69, 402, 474
NheI GCTAGC 1 cut(s) 461
NlaIII CATG 4 cut(s) 128, 413, 500, 521
NlaIV GGNNCC 2 cut(s) 6, 82
NmuCI GTSAC 3 cut(s) 218, 223, 588
NspI RCATGY 2 cut(s) 413, 500
NspV TTCGAA 1 cut(s) 512
OliI CACNNNNGTG 1 cut(s) 548
PaeI GCATGC 2 cut(s) 413, 500
PcsI WCGNNNNNNNCGW 2 cut(s) 144, 156
PfeI GAWTC 1 cut(s) 98
PinAI ACCGGT 1 cut(s) 74
PkrI GCNGC 1 cut(s) 415
PleI GAGTC 3 cut(s) 105, 530, 742
PpsI GAGTC 3 cut(s) 105, 530, 742
Ppu21I YACGTR 2 cut(s) 44, 575
PpuMI RGGWCCY 1 cut(s) 367
Psp124BI GAGCTC 1 cut(s) 558
Psp5II RGGWCCY 1 cut(s) 367
Psp6I CCWGG 1 cut(s) 396
PspGI CCWGG 1 cut(s) 396
PspN4I GGNNCC 2 cut(s) 6, 82
PspPI GGNCC 2 cut(s) 166, 367
PspPPI RGGWCCY 1 cut(s) 367
PstNI CAGNNNCTG 1 cut(s) 710
RsaI GTAC 2 cut(s) 503, 566
RsaNI GTAC 2 cut(s) 502, 565
RseI CAYNNNNRTG 1 cut(s) 548
SacI GAGCTC 1 cut(s) 558
SaqAI TTAA 6 cut(s) 17, 23, 342, 630, 672, 820
SatI GCNGC 1 cut(s) 414
Sau3AI GATC 3 cut(s) 69, 402, 474
Sau96I GGNCC 2 cut(s) 166, 367
SchI GAGTC 3 cut(s) 105, 531, 743
ScrFI CCNGG 1 cut(s) 398
SduI GDGCHC 2 cut(s) 534, 558
SfaNI GCATC 2 cut(s) 99, 318
SfuI TTCGAA 1 cut(s) 512
SgrAI CRCCGGYG 1 cut(s) 74
SinI GGWCC 1 cut(s) 367
SmiMI CAYNNNNRTG 1 cut(s) 548
SnaBI TACGTA 1 cut(s) 44
SphI GCATGC 2 cut(s) 413, 500
Sse9I AATT 4 cut(s) 19, 580, 623, 757
SsiI CCGC 4 cut(s) 105, 413, 548, 692
SspMI CTAG 3 cut(s) 284, 435, 462
SstI GAGCTC 1 cut(s) 558
StyD4I CCNGG 1 cut(s) 396
TaaI ACNGT 2 cut(s) 28, 594
TaiI ACGT 6 cut(s) 46, 153, 225, 243, 577, 611
TaqI TCGA 5 cut(s) 243, 360, 473, 512, 525
TasI AATT 4 cut(s) 19, 580, 623, 757
TauI GCSGC 1 cut(s) 416
TfiI GAWTC 1 cut(s) 98
Tru1I TTAA 6 cut(s) 17, 23, 342, 630, 672, 820
Tru9I TTAA 6 cut(s) 17, 23, 342, 630, 672, 820
TscAI CASTG 4 cut(s) 72, 91, 319, 463
TseFI GTSAC 3 cut(s) 218, 223, 588
Tsp45I GTSAC 3 cut(s) 218, 223, 588
TspDTI ATGAA 3 cut(s) 199, 246, 657
TspGWI ACGGA 2 cut(s) 163, 582
TspRI CASTG 4 cut(s) 72, 91, 319, 463
VpaK11BI GGWCC 1 cut(s) 367
XapI RAATTY 2 cut(s) 19, 623
XceI RCATGY 2 cut(s) 413, 500
XspI CTAG 3 cut(s) 284, 435, 462
ZraI GACGTC 2 cut(s) 241, 609
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.