Rorug05G0234700

Secoisolariciresinol dehydrogenase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0001933
Physical Location & Seq
Forward (+)
24593635 .. 24597831
4197 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0234700.1

Sequence Viewer

Length: 1260 bp
ATGGGGTCTGCAAACATGGAGAAGAAATGGCTATTCCCTCTTGTCATAAGCTCTTGTGTATGCTTATTCCTTCTAGCCACTTCCTTGAACTTGGGTCTTGTCTCTTCACCACACAAACTCTTCTCCTCGATGTTACAATCTCGAGTAGCAACAAACCAAACCGGTGCAGTTTTCGCTGAGGCAAAAGTTTCACAAGCTCCACCCCCTCCCGCAGTACCAGCTATTCCTCGTTTTGCTTATCTAATTTCAGGGTCAAAAGGGGATATGGAAAAGCTTTGGAGGACCCTTAAGGCTCTATACCATCCTTTGAATCACTATGTTCTTCATTTAGACCTTGAGGCACCAGTAGCAGAAAGATTGGAGCTTGCTTCACGAGTGCAAAACGAAACAATGTTTAATAATGTTGGGAATGTATTTATGATAAAAAAGGCTAATATGGTCACTTATAGAGGACCAACAATGGTTGCTAATACTCTTCATGCCTGTGCCATTCTGCTCAGGAGGAGTAAGGATTGGGATTGGTTTATCAACCTCAGCGCCTCGGATTATCCGCTTGTTACTCAAGATGATCTTCTTCACACCTTTTCAGGTCTAAATCGAGAACTGAATTTCATTGAGCATACTAGCCAGCTTGGTTGGAAGGAGGAAAAAAGAGCAATGCCTTTAATTCTAGACCCTGGTCTCTACTCAGCAAATAAACAAGATGTCCTTTGGGTTACACCCCGGCGAACATTGCCAACTTCCTTTAAATTGTTTACTGGTTCGGCATGGATGGTACTGTCACGATGGTTTGTTGAGTACTGCGTTTGGGGTTGGGACAATCTACCAAGGACCCTTCTCATGTATTACACAAACTTTGTCTCCTCGCCTGAAGGCTACTTTCACACTGTTATATGCAATGTGCCAGAGTTTGCTAAAACTGCAGTCAACCATGATTTGCACTATATTTCTTGGGATATTCCTCCCAAACAGCATCCTCACACCCTTAACATAAGTGACACAAGCAAGATGATTGGAAGTGGTGCTGCCTTTGCCCGGAAATTCAAACACAATGACCCTGCCCTAGATAAGATTGATAAAGAGTTACTTCACAGAAGAAAAGATGGCTTCACTCCTGGTGGTTGGTGTGCTGGAAAACCGAAATGCACTAGGGTTGGGAACCCGAACGAGATCAAACCAGGTCAAGGGGCTGAAAGGCTTCGTGCCCTTGTGGATAGGCTGACATTGACAGCTAAGTTTGGTCAAAACCAGTGTAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
adh_short_C2

Protein Analysis

419

Amino Acids

47.35

Weight (kDa)

9.32

Isoelectric Point (pI)

41.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 78 - 337 1e-80 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 340
AciI CCGC 2 cut(s) 210, 551
AcsI RAATTY 2 cut(s) 607, 1040
AcuI CTGAAG 1 cut(s) 891
AfaI GTAC 3 cut(s) 216, 777, 800
AfiI CCNNNNNNNGG 2 cut(s) 1035, 1184
AflII CTTAAG 1 cut(s) 287
AgeI ACCGGT 1 cut(s) 161
AgsI TTSAA 3 cut(s) 88, 310, 1045
AjnI CCWGG 3 cut(s) 676, 1114, 1177
AluBI AGCT 7 cut(s) 51, 197, 221, 274, 364, 631, 1232
AluI AGCT 7 cut(s) 51, 197, 221, 274, 364, 631, 1232
Alw26I GTCTC 3 cut(s) 106, 686, 865
Ama87I CYCGRG 1 cut(s) 141
ApeKI GCWGC 1 cut(s) 1025
ApoI RAATTY 2 cut(s) 607, 1040
AsiGI ACCGGT 1 cut(s) 161
Asp700I GAANNNNTTC 1 cut(s) 1197
AspLEI GCGC 1 cut(s) 539
AspS9I GGNCC 3 cut(s) 282, 452, 831
AsuC2I CCSGG 2 cut(s) 724, 1036
AsuHPI GGTGA 1 cut(s) 99
AvaI CYCGRG 1 cut(s) 141
AvaII GGWCC 3 cut(s) 282, 452, 831
BaeGI GKGCMC 1 cut(s) 1207
BanI GGYRCC 1 cut(s) 340
BauI CACGAG 1 cut(s) 372
BbvCI CCTCAGC 2 cut(s) 177, 533
BbvI GCAGC 1 cut(s) 1012
BccI CCATC 4 cut(s) 309, 766, 780, 1097
BciT130I CCWGG 3 cut(s) 678, 1116, 1179
BcnI CCSGG 2 cut(s) 724, 1036
BcoDI GTCTC 3 cut(s) 106, 686, 865
BfaI CTAG 5 cut(s) 74, 624, 671, 1064, 1149
BfmI CTRYAG 1 cut(s) 921
BfoI RGCGCY 1 cut(s) 540
BfrI CTTAAG 1 cut(s) 287
BisI GCNGC 1 cut(s) 1026
BlsI GCNGC 1 cut(s) 1027
BmcAI AGTACT 1 cut(s) 800
Bme1390I CCNGG 5 cut(s) 678, 724, 1036, 1116, 1179
Bme18I GGWCC 3 cut(s) 282, 452, 831
BmeT110I CYCGRG 1 cut(s) 141
BmgT120I GGNCC 3 cut(s) 282, 452, 831
BmiI GGNNCC 4 cut(s) 284, 342, 833, 1160
BmrFI CCNGG 5 cut(s) 678, 724, 1036, 1116, 1179
BmsI GCATC 1 cut(s) 982
BoxI GACNNNNGTC 1 cut(s) 678
Bpu10I CCTNAGC 3 cut(s) 177, 497, 533
BpuEI CTTGAG 2 cut(s) 356, 546
BpuMI CCSGG 2 cut(s) 724, 1036
BsaI GGTCTC 1 cut(s) 686
BsaJI CCNNGG 4 cut(s) 540, 676, 722, 827
BsaWI WCCGGW 1 cut(s) 161
BsaXI ACNNNNNCTCC 2 cut(s) 845, 875
Bsc4I CCNNNNNNNGG 2 cut(s) 1035, 1184
Bse118I RCCGGY 1 cut(s) 161
Bse1I ACTGG 3 cut(s) 344, 763, 1249
Bse3DI GCAATG 3 cut(s) 663, 731, 904
BseBI CCWGG 3 cut(s) 678, 1116, 1179
BseDI CCNNGG 4 cut(s) 540, 676, 722, 827
BseGI GGATG 3 cut(s) 301, 777, 973
BseLI CCNNNNNNNGG 2 cut(s) 1035, 1184
BseMI GCAATG 3 cut(s) 663, 731, 904
BseMII CTCAG 4 cut(s) 168, 511, 547, 702
BseNI ACTGG 3 cut(s) 344, 763, 1249
BseRI GAGGAG 3 cut(s) 115, 517, 853
BseSI GKGCMC 1 cut(s) 1207
BseXI GCAGC 1 cut(s) 1012
BsgI GTGCAG 1 cut(s) 186
BshNI GGYRCC 1 cut(s) 340
BshTI ACCGGT 1 cut(s) 161
BsiHKCI CYCGRG 1 cut(s) 141
BsiSI CCGG 3 cut(s) 162, 724, 1036
BslFI GGGAC 1 cut(s) 830
BslI CCNNNNNNNGG 2 cut(s) 1035, 1184
BsmAI GTCTC 3 cut(s) 106, 686, 865
BsmFI GGGAC 1 cut(s) 830
Bso31I GGTCTC 1 cut(s) 686
BsoBI CYCGRG 1 cut(s) 141
Bsp1286I GDGCHC 1 cut(s) 1207
Bsp143I GATC 2 cut(s) 568, 1170
BspACI CCGC 2 cut(s) 210, 551
BspCNI CTCAG 4 cut(s) 169, 510, 546, 701
BspLI GGNNCC 4 cut(s) 284, 342, 833, 1160
BspMAI CTGCAG 1 cut(s) 925
BspT107I GGYRCC 1 cut(s) 340
BspTI CTTAAG 1 cut(s) 287
BspTNI GGTCTC 1 cut(s) 686
BsrDI GCAATG 3 cut(s) 663, 731, 904
BsrFI RCCGGY 1 cut(s) 161
BsrI ACTGG 3 cut(s) 344, 763, 1249
BssAI RCCGGY 1 cut(s) 161
BssECI CCNNGG 4 cut(s) 540, 676, 722, 827
BssMI GATC 2 cut(s) 568, 1170
BssSI CACGAG 1 cut(s) 372
BssT1I CCWWGG 1 cut(s) 827
Bst2BI CACGAG 1 cut(s) 372
Bst2UI CCWGG 3 cut(s) 678, 1116, 1179
Bst4CI ACNGT 2 cut(s) 780, 889
Bst6I CTCTTC 3 cut(s) 109, 125, 480
BstAFI CTTAAG 1 cut(s) 287
BstC8I GCNNGC 2 cut(s) 366, 629
BstDEI CTNAG 5 cut(s) 177, 497, 533, 688, 1233
BstF5I GGATG 3 cut(s) 301, 777, 973
BstH2I RGCGCY 1 cut(s) 540
BstHHI GCGC 1 cut(s) 539
BstKTI GATC 2 cut(s) 571, 1173
BstMAI GTCTC 3 cut(s) 106, 686, 865
BstMBI GATC 2 cut(s) 568, 1170
BstMWI GCNNNNNNNGC 6 cut(s) 173, 218, 347, 733, 920, 1031
BstNI CCWGG 3 cut(s) 678, 1116, 1179
BstPAI GACNNNNGTC 1 cut(s) 678
BstSCI CCNGG 5 cut(s) 676, 722, 1034, 1114, 1177
BstSFI CTRYAG 1 cut(s) 921
BstSLI GKGCMC 1 cut(s) 1207
BstV1I GCAGC 1 cut(s) 1012
BtsCI GGATG 3 cut(s) 301, 777, 973
BtsIMutI CAGTG 2 cut(s) 885, 1256
Cac8I GCNNGC 2 cut(s) 366, 629
CfoI GCGC 1 cut(s) 539
Cfr10I RCCGGY 1 cut(s) 161
Cfr13I GGNCC 3 cut(s) 282, 452, 831
CsiI ACCWGGT 1 cut(s) 1177
Csp6I GTAC 3 cut(s) 215, 776, 799
CspAI ACCGGT 1 cut(s) 161
CviAII CATG 5 cut(s) 16, 479, 768, 841, 932
CviQI GTAC 3 cut(s) 215, 776, 799
DdeI CTNAG 5 cut(s) 177, 497, 533, 688, 1233
DpnI GATC 2 cut(s) 570, 1172
DpnII GATC 2 cut(s) 568, 1170
DraI TTTAAA 1 cut(s) 748
Eam1104I CTCTTC 3 cut(s) 109, 125, 480
EarI CTCTTC 3 cut(s) 109, 125, 480
Eco130I CCWWGG 1 cut(s) 827
Eco31I GGTCTC 1 cut(s) 686
Eco47I GGWCC 3 cut(s) 282, 452, 831
Eco57I CTGAAG 1 cut(s) 891
Eco88I CYCGRG 1 cut(s) 141
EcoO109I RGGNCCY 2 cut(s) 282, 831
EcoRII CCWGG 3 cut(s) 676, 1114, 1177
EcoT14I CCWWGG 1 cut(s) 827
ErhI CCWWGG 1 cut(s) 827
FaeI CATG 5 cut(s) 19, 482, 771, 844, 935
FalI AAGNNNNNCTT 6 cut(s) 555, 587, 693, 725, 1071, 1103
FaqI GGGAC 1 cut(s) 830
FatI CATG 5 cut(s) 15, 478, 767, 840, 931
FauI CCCGC 1 cut(s) 217
Fnu4HI GCNGC 1 cut(s) 1026
FokI GGATG 3 cut(s) 288, 784, 960
Fsp4HI GCNGC 1 cut(s) 1026
FspBI CTAG 5 cut(s) 74, 624, 671, 1064, 1149
GlaI GCGC 1 cut(s) 538
GluI GCNGC 1 cut(s) 1026
HaeII RGCGCY 1 cut(s) 540
HapII CCGG 3 cut(s) 162, 724, 1036
HhaI GCGC 1 cut(s) 539
Hin1II CATG 5 cut(s) 19, 482, 771, 844, 935
Hin6I GCGC 1 cut(s) 537
HinP1I GCGC 1 cut(s) 537
HincII GTYRAC 1 cut(s) 928
HindII GTYRAC 1 cut(s) 928
HindIII AAGCTT 1 cut(s) 272
HinfI GANTC 1 cut(s) 310
HpaII CCGG 3 cut(s) 162, 724, 1036
HphI GGTGA 1 cut(s) 99
Hpy166II GTNNAC 2 cut(s) 756, 928
Hpy188I TCNGA 1 cut(s) 544
Hpy188III TCNNGA 7 cut(s) 141, 372, 499, 563, 599, 671, 783
Hpy8I GTNNAC 2 cut(s) 756, 928
HpyAV CCTTC 4 cut(s) 80, 634, 845, 866
HpyCH4III ACNGT 2 cut(s) 780, 889
HpyCH4V TGCA 7 cut(s) 11, 167, 379, 897, 923, 940, 1146
HpyF10VI GCNNNNNNNGC 6 cut(s) 173, 218, 347, 733, 920, 1031
HpyF3I CTNAG 5 cut(s) 177, 497, 533, 688, 1233
Hsp92II CATG 5 cut(s) 19, 482, 771, 844, 935
HspAI GCGC 1 cut(s) 537
Kzo9I GATC 2 cut(s) 568, 1170
LmnI GCTCC 2 cut(s) 202, 361
Lsp1109I GCAGC 1 cut(s) 1012
LweI GCATC 1 cut(s) 982
MabI ACCWGGT 1 cut(s) 1177
MaeI CTAG 5 cut(s) 74, 624, 671, 1064, 1149
MaeIII GTNAC 7 cut(s) 132, 439, 556, 715, 780, 995, 1083
MalI GATC 2 cut(s) 570, 1172
MboI GATC 2 cut(s) 568, 1170
MboII GAAGA 8 cut(s) 34, 96, 112, 314, 467, 563, 566, 1107
MhlI GDGCHC 1 cut(s) 1207
MluCI AATT 5 cut(s) 243, 607, 666, 749, 1040
MmeI TCCRAC 1 cut(s) 617
MroXI GAANNNNTTC 1 cut(s) 1197
MseI TTAA 5 cut(s) 288, 396, 665, 747, 987
MslI CAYNNNNRTG 1 cut(s) 483
MspCI CTTAAG 1 cut(s) 287
MspI CCGG 3 cut(s) 162, 724, 1036
MspR9I CCNGG 5 cut(s) 678, 724, 1036, 1116, 1179
MvaI CCWGG 3 cut(s) 678, 1116, 1179
MwoI GCNNNNNNNGC 6 cut(s) 173, 218, 347, 733, 920, 1031
NciI CCSGG 2 cut(s) 724, 1036
NdeII GATC 2 cut(s) 568, 1170
NlaIII CATG 5 cut(s) 19, 482, 771, 844, 935
NlaIV GGNNCC 4 cut(s) 284, 342, 833, 1160
NmuCI GTSAC 3 cut(s) 439, 780, 995
PaeR7I CTCGAG 1 cut(s) 141
PdmI GAANNNNTTC 1 cut(s) 1197
PfeI GAWTC 1 cut(s) 310
PinAI ACCGGT 1 cut(s) 161
PkrI GCNGC 1 cut(s) 1027
PpuMI RGGWCCY 2 cut(s) 282, 831
PshAI GACNNNNGTC 1 cut(s) 678
Psp5II RGGWCCY 2 cut(s) 282, 831
Psp6I CCWGG 3 cut(s) 676, 1114, 1177
PspGI CCWGG 3 cut(s) 676, 1114, 1177
PspN4I GGNNCC 4 cut(s) 284, 342, 833, 1160
PspPI GGNCC 3 cut(s) 282, 452, 831
PspPPI RGGWCCY 2 cut(s) 282, 831
PstI CTGCAG 1 cut(s) 925
RsaI GTAC 3 cut(s) 216, 777, 800
RsaNI GTAC 3 cut(s) 215, 776, 799
RseI CAYNNNNRTG 1 cut(s) 483
SaqAI TTAA 5 cut(s) 288, 396, 665, 747, 987
SatI GCNGC 1 cut(s) 1026
Sau3AI GATC 2 cut(s) 568, 1170
Sau96I GGNCC 3 cut(s) 282, 452, 831
ScaI AGTACT 1 cut(s) 800
ScrFI CCNGG 5 cut(s) 678, 724, 1036, 1116, 1179
SduI GDGCHC 1 cut(s) 1207
SexAI ACCWGGT 1 cut(s) 1177
SfaNI GCATC 1 cut(s) 982
SfcI CTRYAG 1 cut(s) 921
Sfr274I CTCGAG 1 cut(s) 141
SinI GGWCC 3 cut(s) 282, 452, 831
SlaI CTCGAG 1 cut(s) 141
SmiMI CAYNNNNRTG 1 cut(s) 483
SmlI CTYRAG 4 cut(s) 141, 287, 335, 561
SmoI CTYRAG 4 cut(s) 141, 287, 335, 561
Sse9I AATT 5 cut(s) 243, 607, 666, 749, 1040
SsiI CCGC 2 cut(s) 210, 551
SspMI CTAG 5 cut(s) 74, 624, 671, 1064, 1149
StyD4I CCNGG 5 cut(s) 676, 722, 1034, 1114, 1177
StyI CCWWGG 1 cut(s) 827
TaaI ACNGT 2 cut(s) 780, 889
TaqI TCGA 3 cut(s) 128, 142, 598
TasI AATT 5 cut(s) 243, 607, 666, 749, 1040
TatI WGTACW 1 cut(s) 798
TfiI GAWTC 1 cut(s) 310
Tru1I TTAA 5 cut(s) 288, 396, 665, 747, 987
Tru9I TTAA 5 cut(s) 288, 396, 665, 747, 987
TscAI CASTG 2 cut(s) 892, 1256
TseFI GTSAC 3 cut(s) 439, 780, 995
TseI GCWGC 1 cut(s) 1025
Tsp45I GTSAC 3 cut(s) 439, 780, 995
TspDTI ATGAA 3 cut(s) 314, 467, 601
TspRI CASTG 2 cut(s) 892, 1256
Vha464I CTTAAG 1 cut(s) 287
VpaK11BI GGWCC 3 cut(s) 282, 452, 831
XapI RAATTY 2 cut(s) 607, 1040
XbaI TCTAGA 1 cut(s) 670
XhoI CTCGAG 1 cut(s) 141
XmnI GAANNNNTTC 1 cut(s) 1197
XspI CTAG 5 cut(s) 74, 624, 671, 1064, 1149
ZrmI AGTACT 1 cut(s) 800
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.