Rh5CG348600

Secoisolariciresinol dehydrogenase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0001933
Physical Location & Seq
Forward (+)
40159143 .. 40160051
909 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG348600.1

Sequence Viewer

Length: 813 bp
ATGGCGAGTTTTCCATTACTCTCGACTGCTGCAAGAAGGCTCGAAGGTATGGTAGCAGTGATCACCGGCGGAGCCAGTGGCATCGGAGAATCCACTACTAGACTCTTCTCCAGACACGGCGCTAAAGTTGTGATCGCTGACGTGCAAGATGACTTGGGCCAATCCGTTTGCAGAGACCTGAACCCTTCCTCCACCACCTTTATCCATTGTGATGTGACGAACGAAAAAGACGTCGAAAATGCAGTGAACATAGCCACCGCCAAGTATGGAAAGTTAGACATTATGTTCAACAATGCTGGCATTAGCGACGTGGCCAAGCTGAACATCCTCGACAACAACAAGGCCGATTTCGAGCAGGTCCTCAGAGTGAACGTGGTAGGTGTCTTCCTAGGGATCAAGCACGCGGCTCGGGTGATGATGGGAGCTCGAAAAGGGACTATTATCAACACGGCTAGCGTTTGCTCGATTATAGGGGGCGGTGCGTCGCATGCGTACACGAGTTCGAAGCATGGAGTCGAGGGGCTCACGAAGAACACGGCGGTAGAGCTCGGACAATACGGAATACGTGTGAATTGTGTATTGCCGTATTTGGTGGAGACGTTGTTGACCAAGGATTTCTTTAAGCTTGACGATGAGGGAGTCGGTGGTGTTTACTCCAACCTCAAAGGGGCGGTGCTGAAGGTGGAAGATGTAGCGGAGGCTGTTCTTTACTTGGGGAGTGATGAGTCCAAGTATGTTAGTGGGCATAATTTGGTAGTAGATGGAGGCTACACTATTGTCAATGGCAGGTTCTGCCTGTTTAGGCAATCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
adh_short_C2

Protein Analysis

270

Amino Acids

28.73

Weight (kDa)

6.3

Isoelectric Point (pI)

19.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short PF00106 18 - 206 1.1e-50 short chain dehydrogenase
KR PF08659 19 - 177 1e-08 KR domain
adh_short_C2 PF13561 25 - 258 4e-63 Enoyl-(Acyl carrier protein) reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 234
Acc36I ACCTGC 2 cut(s) 346, 777
AccII CGCG 1 cut(s) 404
AciI CCGC 7 cut(s) 69, 258, 404, 477, 539, 671, 695
AclWI GGATC 1 cut(s) 401
AcoI YGGCCR 1 cut(s) 312
AcuI CTGAAG 1 cut(s) 698
AcyI GRCGYC 1 cut(s) 231
AfaI GTAC 1 cut(s) 494
AfiI CCNNNNNNNGG 1 cut(s) 667
AflIII ACRYGT 1 cut(s) 565
AgsI TTSAA 1 cut(s) 289
AjiI CACGTC 2 cut(s) 142, 310
AloI GAACNNNNNNTCC 2 cut(s) 173, 205
AluBI AGCT 4 cut(s) 319, 425, 547, 625
AluI AGCT 4 cut(s) 319, 425, 547, 625
Alw21I GWGCWC 2 cut(s) 427, 549
Alw26I GTCTC 2 cut(s) 168, 590
AlwI GGATC 1 cut(s) 401
AlwNI CAGNNNCTG 1 cut(s) 792
Ama87I CYCGRG 1 cut(s) 408
AoxI GGCC 3 cut(s) 157, 312, 342
ApeKI GCWGC 1 cut(s) 29
ArsI GACNNNNNNTTYG 2 cut(s) 216, 248
AspA2I CCTAGG 1 cut(s) 388
AspLEI GCGC 1 cut(s) 122
AspS9I GGNCC 2 cut(s) 157, 358
AsuHPI GGTGA 2 cut(s) 55, 424
AsuII TTCGAA 1 cut(s) 503
AsuNHI GCTAGC 1 cut(s) 452
AvaI CYCGRG 1 cut(s) 408
AvaII GGWCC 1 cut(s) 358
AvrII CCTAGG 1 cut(s) 388
BalI TGGCCA 1 cut(s) 314
BanII GRGCYC 3 cut(s) 427, 525, 549
BauI CACGAG 1 cut(s) 496
BbsI GAAGAC 1 cut(s) 376
Bbv12I GWGCWC 2 cut(s) 427, 549
BbvI GCAGC 1 cut(s) 16
BccI CCATC 2 cut(s) 412, 755
BceAI ACGGC 4 cut(s) 133, 465, 552, 568
BcgI CGANNNNNNTGC 2 cut(s) 389, 423
BclI TGATCA 1 cut(s) 60
BcoDI GTCTC 2 cut(s) 168, 590
BfaI CTAG 3 cut(s) 99, 389, 453
BfoI RGCGCY 1 cut(s) 123
BfuAI ACCTGC 2 cut(s) 346, 777
BisI GCNGC 2 cut(s) 30, 405
BlnI CCTAGG 1 cut(s) 388
BlsI GCNGC 2 cut(s) 31, 406
Bme18I GGWCC 1 cut(s) 358
BmeT110I CYCGRG 1 cut(s) 408
BmgBI CACGTC 2 cut(s) 142, 310
BmgT120I GGNCC 2 cut(s) 157, 358
BmiI GGNNCC 1 cut(s) 73
BmsI GCATC 1 cut(s) 90
BmtI GCTAGC 1 cut(s) 456
BpiI GAAGAC 1 cut(s) 376
BpmI CTGGAG 1 cut(s) 94
Bpu14I TTCGAA 1 cut(s) 503
BsaAI YACGTR 1 cut(s) 566
BsaHI GRCGYC 1 cut(s) 231
BsaI GGTCTC 1 cut(s) 168
BsaJI CCNNGG 2 cut(s) 388, 609
BsaXI ACNNNNNCTCC 4 cut(s) 173, 203, 587, 617
Bsc4I CCNNNNNNNGG 1 cut(s) 667
Bse118I RCCGGY 1 cut(s) 65
Bse1I ACTGG 1 cut(s) 75
BseDI CCNNGG 2 cut(s) 388, 609
BseGI GGATG 1 cut(s) 324
BseLI CCNNNNNNNGG 1 cut(s) 667
BseMII CTCAG 1 cut(s) 376
BseNI ACTGG 1 cut(s) 75
BseXI GCAGC 1 cut(s) 16
Bsh1236I CGCG 1 cut(s) 404
BshFI GGCC 3 cut(s) 159, 314, 344
BsiHKAI GWGCWC 2 cut(s) 427, 549
BsiHKCI CYCGRG 1 cut(s) 408
BsiSI CCGG 1 cut(s) 66
BslFI GGGAC 1 cut(s) 448
BslI CCNNNNNNNGG 1 cut(s) 667
BsmAI GTCTC 2 cut(s) 168, 590
BsmBI CGTCTC 1 cut(s) 590
BsmFI GGGAC 1 cut(s) 448
BsnI GGCC 3 cut(s) 159, 314, 344
Bso31I GGTCTC 1 cut(s) 168
BsoBI CYCGRG 1 cut(s) 408
Bsp119I TTCGAA 1 cut(s) 503
Bsp1286I GDGCHC 3 cut(s) 427, 525, 549
Bsp143I GATC 3 cut(s) 60, 132, 393
BspACI CCGC 7 cut(s) 69, 258, 404, 477, 539, 671, 695
BspANI GGCC 3 cut(s) 159, 314, 344
BspCNI CTCAG 1 cut(s) 375
BspFNI CGCG 1 cut(s) 404
BspLI GGNNCC 1 cut(s) 73
BspMI ACCTGC 2 cut(s) 346, 777
BspOI GCTAGC 1 cut(s) 456
BspPI GGATC 1 cut(s) 401
BspT104I TTCGAA 1 cut(s) 503
BspTNI GGTCTC 1 cut(s) 168
BsrFI RCCGGY 1 cut(s) 65
BsrI ACTGG 1 cut(s) 75
BssAI RCCGGY 1 cut(s) 65
BssECI CCNNGG 2 cut(s) 388, 609
BssMI GATC 3 cut(s) 60, 132, 393
BssNI GRCGYC 1 cut(s) 231
BssSI CACGAG 1 cut(s) 496
BssT1I CCWWGG 2 cut(s) 388, 609
Bst2BI CACGAG 1 cut(s) 496
Bst6I CTCTTC 1 cut(s) 110
BstACI GRCGYC 1 cut(s) 231
BstAPI GCANNNNNTGC 1 cut(s) 792
BstBAI YACGTR 1 cut(s) 566
BstBI TTCGAA 1 cut(s) 503
BstC8I GCNNGC 4 cut(s) 298, 402, 454, 489
BstDEI CTNAG 1 cut(s) 362
BstF5I GGATG 1 cut(s) 324
BstFNI CGCG 1 cut(s) 404
BstH2I RGCGCY 1 cut(s) 123
BstHHI GCGC 1 cut(s) 122
BstKTI GATC 3 cut(s) 63, 135, 396
BstMAI GTCTC 2 cut(s) 168, 590
BstMBI GATC 3 cut(s) 60, 132, 393
BstMWI GCNNNNNNNGC 2 cut(s) 488, 792
BstNSI RCATGY 1 cut(s) 491
BstUI CGCG 1 cut(s) 404
BstV1I GCAGC 1 cut(s) 16
BstV2I GAAGAC 1 cut(s) 376
BsuRI GGCC 3 cut(s) 159, 314, 344
BtrI CACGTC 2 cut(s) 142, 310
BtsCI GGATG 1 cut(s) 324
BtsI GCAGTG 2 cut(s) 63, 249
BtsIMutI CAGTG 3 cut(s) 63, 82, 249
BveI ACCTGC 2 cut(s) 346, 777
Cac8I GCNNGC 4 cut(s) 298, 402, 454, 489
CaiI CAGNNNCTG 1 cut(s) 792
CfoI GCGC 1 cut(s) 122
Cfr10I RCCGGY 1 cut(s) 65
Cfr13I GGNCC 2 cut(s) 157, 358
CseI GACGC 1 cut(s) 471
Csp6I GTAC 1 cut(s) 493
CviAII CATG 2 cut(s) 488, 509
CviQI GTAC 1 cut(s) 493
DdeI CTNAG 1 cut(s) 362
DpnI GATC 3 cut(s) 62, 134, 395
DpnII GATC 3 cut(s) 60, 132, 393
EaeI YGGCCR 1 cut(s) 312
Eam1104I CTCTTC 1 cut(s) 110
EarI CTCTTC 1 cut(s) 110
EciI GGCGGA 1 cut(s) 84
Ecl136II GAGCTC 2 cut(s) 425, 547
Eco130I CCWWGG 2 cut(s) 388, 609
Eco24I GRGCYC 3 cut(s) 427, 525, 549
Eco31I GGTCTC 1 cut(s) 168
Eco47I GGWCC 1 cut(s) 358
Eco53kI GAGCTC 2 cut(s) 425, 547
Eco57I CTGAAG 1 cut(s) 698
Eco88I CYCGRG 1 cut(s) 408
EcoICRI GAGCTC 2 cut(s) 425, 547
EcoO109I RGGNCCY 1 cut(s) 358
EcoT14I CCWWGG 2 cut(s) 388, 609
EcoT38I GRGCYC 3 cut(s) 427, 525, 549
ErhI CCWWGG 2 cut(s) 388, 609
Esp3I CGTCTC 1 cut(s) 590
FaeI CATG 2 cut(s) 491, 512
FaiI YATR 9 cut(s) 50, 251, 267, 284, 470, 489, 510, 735, 747
FalI AAGNNNNNCTT 2 cut(s) 602, 634
FaqI GGGAC 1 cut(s) 448
FatI CATG 2 cut(s) 487, 508
FbaI TGATCA 1 cut(s) 60
Fnu4HI GCNGC 2 cut(s) 30, 405
FokI GGATG 1 cut(s) 311
FriOI GRGCYC 3 cut(s) 427, 525, 549
Fsp4HI GCNGC 2 cut(s) 30, 405
FspBI CTAG 3 cut(s) 99, 389, 453
GlaI GCGC 1 cut(s) 121
GluI GCNGC 2 cut(s) 30, 405
GsuI CTGGAG 1 cut(s) 94
HaeII RGCGCY 1 cut(s) 123
HaeIII GGCC 3 cut(s) 159, 314, 344
HapII CCGG 1 cut(s) 66
HgaI GACGC 1 cut(s) 471
HhaI GCGC 1 cut(s) 122
Hin1I GRCGYC 1 cut(s) 231
Hin1II CATG 2 cut(s) 491, 512
Hin6I GCGC 1 cut(s) 120
HinP1I GCGC 1 cut(s) 120
HincII GTYRAC 1 cut(s) 606
HindII GTYRAC 1 cut(s) 606
HindIII AAGCTT 1 cut(s) 623
HinfI GANTC 5 cut(s) 89, 102, 513, 639, 725
HpaII CCGG 1 cut(s) 66
HphI GGTGA 2 cut(s) 55, 424
Hpy166II GTNNAC 5 cut(s) 247, 370, 495, 606, 652
Hpy188I TCNGA 3 cut(s) 86, 365, 551
Hpy188III TCNNGA 3 cut(s) 22, 111, 526
Hpy8I GTNNAC 5 cut(s) 247, 370, 495, 606, 652
Hpy99I CGWCG 3 cut(s) 236, 311, 487
HpyAV CCTTC 4 cut(s) 30, 38, 195, 673
HpyCH4IV ACGT 6 cut(s) 141, 231, 309, 372, 565, 599
HpyCH4V TGCA 4 cut(s) 32, 145, 171, 242
HpyF10VI GCNNNNNNNGC 2 cut(s) 488, 792
HpyF3I CTNAG 1 cut(s) 362
HpySE526I ACGT 6 cut(s) 141, 231, 309, 372, 565, 599
Hsp92I GRCGYC 1 cut(s) 231
Hsp92II CATG 2 cut(s) 491, 512
HspAI GCGC 1 cut(s) 120
Ksp22I TGATCA 1 cut(s) 60
Kzo9I GATC 3 cut(s) 60, 132, 393
LmnI GCTCC 2 cut(s) 71, 422
LpnPI CCDG 8 cut(s) 79, 88, 124, 191, 282, 341, 772, 809
Lsp1109I GCAGC 1 cut(s) 16
LweI GCATC 1 cut(s) 90
MaeI CTAG 3 cut(s) 99, 389, 453
MaeII ACGT 6 cut(s) 141, 231, 309, 372, 565, 599
MaeIII GTNAC 1 cut(s) 214
MalI GATC 3 cut(s) 62, 134, 395
MboI GATC 3 cut(s) 60, 132, 393
MboII GAAGA 4 cut(s) 97, 376, 541, 698
MhlI GDGCHC 3 cut(s) 427, 525, 549
MlsI TGGCCA 1 cut(s) 314
MluCI AATT 2 cut(s) 571, 748
MluNI TGGCCA 1 cut(s) 314
MlyI GAGTC 4 cut(s) 96, 522, 648, 734
MmeI TCCRAC 1 cut(s) 681
MnlI CCTC 8 cut(s) 199, 338, 371, 511, 628, 671, 691, 758
Mox20I TGGCCA 1 cut(s) 314
MscI TGGCCA 1 cut(s) 314
MseI TTAA 2 cut(s) 621, 811
MslI CAYNNNNRTG 1 cut(s) 210
Msp20I TGGCCA 1 cut(s) 314
MspI CCGG 1 cut(s) 66
MvnI CGCG 1 cut(s) 404
MwoI GCNNNNNNNGC 2 cut(s) 488, 792
NdeII GATC 3 cut(s) 60, 132, 393
NheI GCTAGC 1 cut(s) 452
NlaIII CATG 2 cut(s) 491, 512
NlaIV GGNNCC 1 cut(s) 73
NmuCI GTSAC 1 cut(s) 214
NspI RCATGY 1 cut(s) 491
NspV TTCGAA 1 cut(s) 503
PaeI GCATGC 1 cut(s) 491
PcsI WCGNNNNNNNCGW 1 cut(s) 228
PfeI GAWTC 1 cut(s) 89
PkrI GCNGC 2 cut(s) 31, 406
PleI GAGTC 4 cut(s) 96, 521, 647, 733
PpsI GAGTC 4 cut(s) 96, 521, 647, 733
Ppu21I YACGTR 1 cut(s) 566
PpuMI RGGWCCY 1 cut(s) 358
Psp124BI GAGCTC 2 cut(s) 427, 549
Psp5II RGGWCCY 1 cut(s) 358
PspN4I GGNNCC 1 cut(s) 73
PspPI GGNCC 2 cut(s) 157, 358
PspPPI RGGWCCY 1 cut(s) 358
PstNI CAGNNNCTG 1 cut(s) 792
RsaI GTAC 1 cut(s) 494
RsaNI GTAC 1 cut(s) 493
RseI CAYNNNNRTG 1 cut(s) 210
SacI GAGCTC 2 cut(s) 427, 549
SaqAI TTAA 2 cut(s) 621, 811
SatI GCNGC 2 cut(s) 30, 405
Sau3AI GATC 3 cut(s) 60, 132, 393
Sau96I GGNCC 2 cut(s) 157, 358
SchI GAGTC 4 cut(s) 96, 522, 648, 734
SduI GDGCHC 3 cut(s) 427, 525, 549
SfaNI GCATC 1 cut(s) 90
SfuI TTCGAA 1 cut(s) 503
SgrAI CRCCGGYG 1 cut(s) 65
SinI GGWCC 1 cut(s) 358
SmiMI CAYNNNNRTG 1 cut(s) 210
SphI GCATGC 1 cut(s) 491
Sse9I AATT 2 cut(s) 571, 748
SsiI CCGC 7 cut(s) 69, 258, 404, 477, 539, 671, 695
SspMI CTAG 3 cut(s) 99, 389, 453
SstI GAGCTC 2 cut(s) 427, 549
StyI CCWWGG 2 cut(s) 388, 609
TaiI ACGT 6 cut(s) 144, 234, 312, 375, 568, 602
TaqI TCGA 9 cut(s) 23, 42, 234, 330, 351, 427, 464, 503, 516
TasI AATT 2 cut(s) 571, 748
TauI GCSGC 1 cut(s) 407
TfiI GAWTC 1 cut(s) 89
Tru1I TTAA 2 cut(s) 621, 811
Tru9I TTAA 2 cut(s) 621, 811
TscAI CASTG 3 cut(s) 63, 82, 249
TseFI GTSAC 1 cut(s) 214
TseI GCWGC 1 cut(s) 29
Tsp45I GTSAC 1 cut(s) 214
TspGWI ACGGA 2 cut(s) 154, 573
TspRI CASTG 3 cut(s) 63, 82, 249
VpaK11BI GGWCC 1 cut(s) 358
XceI RCATGY 1 cut(s) 491
XmaJI CCTAGG 1 cut(s) 388
XspI CTAG 3 cut(s) 99, 389, 453
ZraI GACGTC 1 cut(s) 232
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.