Rh5DG333200

Secoisolariciresinol dehydrogenase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0001933
Physical Location & Seq
Forward (+)
44056795 .. 44091581
34787 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG333200.1

Sequence Viewer

Length: 1101 bp
ATGGCGAGTTTCTCGTTACTCTCGGCTATTGCAAGAAGGCTTGAAGGTAAGGTAGCAGTGATCACCGGCGGAGCCAGTGGCATCGGATTATCCACCGCCAGACTCTTCTCCAGACACGGCGCTAAAGTCGTGATCGCCGACGTGCAAGATGACTTGGGCCAATCCGTTTGTAGATATCTGAACCCTTCCTCCACCACCTTCATCCATTGCAACGTGACGAACGAAAAAGACGTCGAAAACGCTGTGAACACAGCCACCGCCAAGTATGGAAAGCTAGACATTATGTTTAACAACGCCGGCTTCAGCGACTTGGCCAAGCCGAACATCCTCGACAACAACAAGGCCGATTTCGAGCAGGTCCTCAGAGTGAAACTGGTTGGTGTCTTCTTGGGGATCAAGCACGCAACTCGGGTGATGATGGGAGCTCGAAAAGGGATTATTATCAACACGGCTAGCGTTTGCTCAGTCATAGGAGGCGCTGCGTCGCATGCGTACACGAGTTCGAAGCATGGAGTCGAGGGGCTCAAGAAGAATACAGCGGTAGAGCTCGTACATGATGATGATGGAGCCGATCATGAACTAAGGGTTTTGTTGCGGACGCTGATACATTCTGCTAGGGTAAGTCCTATAGAGAGGCATAGAGAGAGAGAGAGAGATGGCAAATCAAGGTGCAAAAAAGCGCAAGGAAGAGAATGCTCGGCACATGGCAAATATCCGTCGCCTGATTGTGGCCTGCAATGTGATTTATGTGGTAGTAAGGATGCTCATCTTCCATTCTACTTTTACCTGGAAAAACTGGGTTGCTTTGCTGCTGACATCTACGGCGGATTTGATATGACCACTGGTGGAGTGTGTGGCTATTTACATGACGTAATCTACATTACAAGCTTCGTGCAAGTCATGTCCATAATCTCTGGAAAATTTTGGTATACGTATCTGCTGATACCAGGTTTTGGAGCATACAAATCATTTGGTTTCTTTAGAGGATTTATGTCCCAAAGTTCAGAGGGAGGCTTGGAGGATGAAAAGACACGAAAGAAGAGGGAAAAGATGGAAAAGAAGGCTTCAAGAGCCCAGTTTGTCAAGACAAGAAAAAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
adh_short_C2

Protein Analysis

366

Amino Acids

40.18

Weight (kDa)

9.33

Isoelectric Point (pI)

30.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short PF00106 17 - 184 4.2e-40 short chain dehydrogenase
KR PF08659 20 - 172 3.4e-09 KR domain
adh_short_C2 PF13561 25 - 185 1.4e-38 Enoyl-(Acyl carrier protein) reductase
TMEM208_SND2 PF05620 260 - 355 1.7e-26 SRP-independent targeting protein 2/TMEM208
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 234
Acc36I ACCTGC 1 cut(s) 346
AccB7I CCANNNNNTGG 1 cut(s) 953
AccI GTMKAC 1 cut(s) 929
AciI CCGC 6 cut(s) 69, 96, 258, 539, 595, 825
AclWI GGATC 1 cut(s) 401
AcoI YGGCCR 1 cut(s) 312
AcsI RAATTY 1 cut(s) 920
AcuI CTGAAG 1 cut(s) 286
AcyI GRCGYC 1 cut(s) 231
AfaI GTAC 2 cut(s) 494, 552
AfiI CCNNNNNNNGG 2 cut(s) 728, 953
AgsI TTSAA 2 cut(s) 44, 1068
AjiI CACGTC 1 cut(s) 142
AjnI CCWGG 2 cut(s) 786, 946
AloI GAACNNNNNNTCC 2 cut(s) 173, 205
AluBI AGCT 4 cut(s) 274, 425, 547, 888
AluI AGCT 4 cut(s) 274, 425, 547, 888
Alw21I GWGCWC 2 cut(s) 427, 549
AlwI GGATC 1 cut(s) 401
Ama87I CYCGRG 1 cut(s) 408
AoxI GGCC 4 cut(s) 157, 312, 342, 730
ApeKI GCWGC 2 cut(s) 479, 809
ApoI RAATTY 1 cut(s) 920
ArsI GACNNNNNNTTYG 2 cut(s) 216, 248
AspLEI GCGC 3 cut(s) 122, 479, 682
AspS9I GGNCC 2 cut(s) 157, 358
AsuHPI GGTGA 2 cut(s) 55, 424
AsuII TTCGAA 1 cut(s) 503
AsuNHI GCTAGC 1 cut(s) 452
AvaI CYCGRG 1 cut(s) 408
AvaII GGWCC 1 cut(s) 358
BalI TGGCCA 1 cut(s) 314
BanII GRGCYC 4 cut(s) 427, 525, 549, 1075
BauI CACGAG 1 cut(s) 496
BbsI GAAGAC 1 cut(s) 376
Bbv12I GWGCWC 2 cut(s) 427, 549
BbvI GCAGC 2 cut(s) 466, 796
BccI CCATC 4 cut(s) 412, 557, 650, 1045
BceAI ACGGC 3 cut(s) 133, 465, 838
BcgI CGANNNNNNTGC 2 cut(s) 389, 423
BciT130I CCWGG 2 cut(s) 788, 948
BclI TGATCA 1 cut(s) 60
BfaI CTAG 3 cut(s) 275, 453, 615
BfmI CTRYAG 1 cut(s) 627
BfoI RGCGCY 2 cut(s) 123, 480
BfuAI ACCTGC 1 cut(s) 346
BisI GCNGC 2 cut(s) 480, 810
BlsI GCNGC 2 cut(s) 481, 811
Bme1390I CCNGG 2 cut(s) 788, 948
Bme18I GGWCC 1 cut(s) 358
BmeT110I CYCGRG 1 cut(s) 408
BmgBI CACGTC 1 cut(s) 142
BmgT120I GGNCC 2 cut(s) 157, 358
BmiI GGNNCC 2 cut(s) 73, 568
BmrFI CCNGG 2 cut(s) 788, 948
BmrI ACTGGG 2 cut(s) 806, 1069
BmsI GCATC 2 cut(s) 90, 751
BmtI GCTAGC 1 cut(s) 456
BmuI ACTGGG 2 cut(s) 806, 1069
BpiI GAAGAC 1 cut(s) 376
BpmI CTGGAG 1 cut(s) 94
Bpu14I TTCGAA 1 cut(s) 503
BpuEI CTTGAG 1 cut(s) 509
BsaAI YACGTR 1 cut(s) 933
BsaBI GATNNNNATC 2 cut(s) 440, 765
BsaHI GRCGYC 1 cut(s) 231
BsaXI ACNNNNNCTCC 2 cut(s) 173, 203
Bsc4I CCNNNNNNNGG 2 cut(s) 728, 953
Bse118I RCCGGY 2 cut(s) 65, 296
Bse1I ACTGG 5 cut(s) 75, 378, 801, 847, 1075
Bse3DI GCAATG 2 cut(s) 205, 743
Bse8I GATNNNNATC 2 cut(s) 440, 765
BseBI CCWGG 2 cut(s) 788, 948
BseGI GGATG 4 cut(s) 201, 324, 766, 1027
BseJI GATNNNNATC 2 cut(s) 440, 765
BseLI CCNNNNNNNGG 2 cut(s) 728, 953
BseMI GCAATG 2 cut(s) 205, 743
BseMII CTCAG 2 cut(s) 376, 477
BseNI ACTGG 5 cut(s) 75, 378, 801, 847, 1075
BseXI GCAGC 2 cut(s) 466, 796
BshFI GGCC 4 cut(s) 159, 314, 344, 732
BsiHKAI GWGCWC 2 cut(s) 427, 549
BsiHKCI CYCGRG 1 cut(s) 408
BsiSI CCGG 2 cut(s) 66, 297
BslFI GGGAC 1 cut(s) 979
BslI CCNNNNNNNGG 2 cut(s) 728, 953
BsmFI GGGAC 1 cut(s) 979
BsmI GAATGC 1 cut(s) 698
BsnI GGCC 4 cut(s) 159, 314, 344, 732
BsoBI CYCGRG 1 cut(s) 408
Bsp119I TTCGAA 1 cut(s) 503
Bsp1286I GDGCHC 4 cut(s) 427, 525, 549, 1075
Bsp143I GATC 4 cut(s) 60, 132, 393, 571
BspACI CCGC 6 cut(s) 69, 96, 258, 539, 595, 825
BspANI GGCC 4 cut(s) 159, 314, 344, 732
BspCNI CTCAG 2 cut(s) 375, 476
BspHI TCATGA 1 cut(s) 574
BspLI GGNNCC 2 cut(s) 73, 568
BspMI ACCTGC 1 cut(s) 346
BspOI GCTAGC 1 cut(s) 456
BspPI GGATC 1 cut(s) 401
BspT104I TTCGAA 1 cut(s) 503
BsrDI GCAATG 2 cut(s) 205, 743
BsrFI RCCGGY 2 cut(s) 65, 296
BsrI ACTGG 5 cut(s) 75, 378, 801, 847, 1075
BssAI RCCGGY 2 cut(s) 65, 296
BssMI GATC 4 cut(s) 60, 132, 393, 571
BssNAI GTATAC 1 cut(s) 930
BssNI GRCGYC 1 cut(s) 231
BssSI CACGAG 1 cut(s) 496
Bst1107I GTATAC 1 cut(s) 930
Bst2BI CACGAG 1 cut(s) 496
Bst2UI CCWGG 2 cut(s) 788, 948
Bst6I CTCTTC 3 cut(s) 110, 682, 1034
BstACI GRCGYC 1 cut(s) 231
BstBAI YACGTR 1 cut(s) 933
BstBI TTCGAA 1 cut(s) 503
BstC8I GCNNGC 5 cut(s) 298, 402, 454, 489, 734
BstDEI CTNAG 3 cut(s) 362, 463, 581
BstF5I GGATG 4 cut(s) 201, 324, 766, 1027
BstH2I RGCGCY 2 cut(s) 123, 480
BstHHI GCGC 3 cut(s) 122, 479, 682
BstKTI GATC 4 cut(s) 63, 135, 396, 574
BstMBI GATC 4 cut(s) 60, 132, 393, 571
BstMWI GCNNNNNNNGC 2 cut(s) 488, 1070
BstNI CCWGG 2 cut(s) 788, 948
BstNSI RCATGY 1 cut(s) 491
BstSCI CCNGG 2 cut(s) 786, 946
BstSFI CTRYAG 1 cut(s) 627
BstSNI TACGTA 1 cut(s) 933
BstV1I GCAGC 2 cut(s) 466, 796
BstV2I GAAGAC 1 cut(s) 376
BstZ17I GTATAC 1 cut(s) 930
BsuRI GGCC 4 cut(s) 159, 314, 344, 732
BtrI CACGTC 1 cut(s) 142
BtsCI GGATG 4 cut(s) 201, 324, 766, 1027
BtsI GCAGTG 1 cut(s) 63
BtsIMutI CAGTG 3 cut(s) 63, 82, 840
BveI ACCTGC 1 cut(s) 346
Cac8I GCNNGC 5 cut(s) 298, 402, 454, 489, 734
CciI TCATGA 1 cut(s) 574
CfoI GCGC 3 cut(s) 122, 479, 682
Cfr10I RCCGGY 2 cut(s) 65, 296
Cfr13I GGNCC 2 cut(s) 157, 358
CseI GACGC 2 cut(s) 471, 607
CsiI ACCWGGT 1 cut(s) 946
Csp6I GTAC 2 cut(s) 493, 551
CviAII CATG 7 cut(s) 488, 509, 554, 575, 704, 866, 901
CviQI GTAC 2 cut(s) 493, 551
DdeI CTNAG 3 cut(s) 362, 463, 581
DpnI GATC 4 cut(s) 62, 134, 395, 573
DpnII GATC 4 cut(s) 60, 132, 393, 571
EaeI YGGCCR 1 cut(s) 312
Eam1104I CTCTTC 3 cut(s) 110, 682, 1034
EarI CTCTTC 3 cut(s) 110, 682, 1034
EciI GGCGGA 2 cut(s) 84, 840
Ecl136II GAGCTC 2 cut(s) 425, 547
Eco105I TACGTA 1 cut(s) 933
Eco24I GRGCYC 4 cut(s) 427, 525, 549, 1075
Eco32I GATATC 1 cut(s) 176
Eco47I GGWCC 1 cut(s) 358
Eco53kI GAGCTC 2 cut(s) 425, 547
Eco57I CTGAAG 1 cut(s) 286
Eco88I CYCGRG 1 cut(s) 408
EcoICRI GAGCTC 2 cut(s) 425, 547
EcoO109I RGGNCCY 1 cut(s) 358
EcoRII CCWGG 2 cut(s) 786, 946
EcoRV GATATC 1 cut(s) 176
EcoT38I GRGCYC 4 cut(s) 427, 525, 549, 1075
FaeI CATG 7 cut(s) 491, 512, 557, 578, 707, 869, 904
FaqI GGGAC 1 cut(s) 979
FatI CATG 7 cut(s) 487, 508, 553, 574, 703, 865, 900
FbaI TGATCA 1 cut(s) 60
FblI GTMKAC 1 cut(s) 929
Fnu4HI GCNGC 2 cut(s) 480, 810
FokI GGATG 4 cut(s) 188, 311, 773, 1034
FriOI GRGCYC 4 cut(s) 427, 525, 549, 1075
Fsp4HI GCNGC 2 cut(s) 480, 810
FspBI CTAG 3 cut(s) 275, 453, 615
GlaI GCGC 3 cut(s) 121, 478, 681
GluI GCNGC 2 cut(s) 480, 810
GsuI CTGGAG 1 cut(s) 94
HaeII RGCGCY 2 cut(s) 123, 480
HaeIII GGCC 4 cut(s) 159, 314, 344, 732
HapII CCGG 2 cut(s) 66, 297
HgaI GACGC 2 cut(s) 471, 607
HhaI GCGC 3 cut(s) 122, 479, 682
Hin1I GRCGYC 1 cut(s) 231
Hin1II CATG 7 cut(s) 491, 512, 557, 578, 707, 869, 904
Hin6I GCGC 3 cut(s) 120, 477, 680
HinP1I GCGC 3 cut(s) 120, 477, 680
HindIII AAGCTT 1 cut(s) 886
HinfI GANTC 2 cut(s) 102, 513
HpaII CCGG 2 cut(s) 66, 297
HphI GGTGA 2 cut(s) 55, 424
Hpy166II GTNNAC 3 cut(s) 247, 495, 930
Hpy188I TCNGA 4 cut(s) 86, 180, 365, 1006
Hpy188III TCNNGA 7 cut(s) 111, 130, 526, 575, 915, 1068, 1084
Hpy8I GTNNAC 3 cut(s) 247, 495, 930
Hpy99I CGWCG 4 cut(s) 143, 236, 487, 721
HpyAV CCTTC 5 cut(s) 30, 38, 195, 208, 1054
HpyCH4IV ACGT 5 cut(s) 141, 213, 231, 870, 932
HpyCH4V TGCA 6 cut(s) 32, 145, 210, 672, 736, 895
HpyF10VI GCNNNNNNNGC 2 cut(s) 488, 1070
HpyF3I CTNAG 3 cut(s) 362, 463, 581
HpySE526I ACGT 5 cut(s) 141, 213, 231, 870, 932
Hsp92I GRCGYC 1 cut(s) 231
Hsp92II CATG 7 cut(s) 491, 512, 557, 578, 707, 869, 904
HspAI GCGC 3 cut(s) 120, 477, 680
KroI GCCGGC 1 cut(s) 296
KroNI GCCGGC 1 cut(s) 298
Ksp22I TGATCA 1 cut(s) 60
Kzo9I GATC 4 cut(s) 60, 132, 393, 571
LmnI GCTCC 4 cut(s) 71, 422, 566, 956
Lsp1109I GCAGC 2 cut(s) 466, 796
LweI GCATC 2 cut(s) 90, 751
MabI ACCWGGT 1 cut(s) 946
MaeI CTAG 3 cut(s) 275, 453, 615
MaeII ACGT 5 cut(s) 141, 213, 231, 870, 932
MaeIII GTNAC 2 cut(s) 15, 214
MalI GATC 4 cut(s) 62, 134, 395, 573
MboI GATC 4 cut(s) 60, 132, 393, 571
MboII GAAGA 6 cut(s) 97, 376, 541, 699, 761, 1051
MhlI GDGCHC 4 cut(s) 427, 525, 549, 1075
MlsI TGGCCA 1 cut(s) 314
MluCI AATT 1 cut(s) 920
MluNI TGGCCA 1 cut(s) 314
MlyI GAGTC 2 cut(s) 96, 522
Mox20I TGGCCA 1 cut(s) 314
MroNI GCCGGC 1 cut(s) 296
MscI TGGCCA 1 cut(s) 314
MseI TTAA 1 cut(s) 288
MslI CAYNNNNRTG 1 cut(s) 558
Msp20I TGGCCA 1 cut(s) 314
MspA1I CMGCKG 1 cut(s) 539
MspI CCGG 2 cut(s) 66, 297
MspR9I CCNGG 2 cut(s) 788, 948
Mva1269I GAATGC 1 cut(s) 698
MvaI CCWGG 2 cut(s) 788, 948
MwoI GCNNNNNNNGC 2 cut(s) 488, 1070
NaeI GCCGGC 1 cut(s) 298
NdeII GATC 4 cut(s) 60, 132, 393, 571
NgoMIV GCCGGC 1 cut(s) 296
NheI GCTAGC 1 cut(s) 452
NlaIII CATG 7 cut(s) 491, 512, 557, 578, 707, 869, 904
NlaIV GGNNCC 2 cut(s) 73, 568
NmeAIII GCCGAG 1 cut(s) 677
NmuCI GTSAC 1 cut(s) 214
NspI RCATGY 1 cut(s) 491
NspV TTCGAA 1 cut(s) 503
PaeI GCATGC 1 cut(s) 491
PagI TCATGA 1 cut(s) 574
PcsI WCGNNNNNNNCGW 3 cut(s) 135, 219, 228
PctI GAATGC 1 cut(s) 698
PdiI GCCGGC 1 cut(s) 298
PflMI CCANNNNNTGG 1 cut(s) 953
PkrI GCNGC 2 cut(s) 481, 811
PleI GAGTC 2 cut(s) 96, 521
PpsI GAGTC 2 cut(s) 96, 521
Ppu21I YACGTR 1 cut(s) 933
PpuMI RGGWCCY 1 cut(s) 358
Psp124BI GAGCTC 2 cut(s) 427, 549
Psp5II RGGWCCY 1 cut(s) 358
Psp6I CCWGG 2 cut(s) 786, 946
PspGI CCWGG 2 cut(s) 786, 946
PspN4I GGNNCC 2 cut(s) 73, 568
PspPI GGNCC 2 cut(s) 157, 358
PspPPI RGGWCCY 1 cut(s) 358
RsaI GTAC 2 cut(s) 494, 552
RsaNI GTAC 2 cut(s) 493, 551
RseI CAYNNNNRTG 1 cut(s) 558
SacI GAGCTC 2 cut(s) 427, 549
SaqAI TTAA 1 cut(s) 288
SatI GCNGC 2 cut(s) 480, 810
Sau3AI GATC 4 cut(s) 60, 132, 393, 571
Sau96I GGNCC 2 cut(s) 157, 358
SchI GAGTC 2 cut(s) 96, 522
ScrFI CCNGG 2 cut(s) 788, 948
SduI GDGCHC 4 cut(s) 427, 525, 549, 1075
SexAI ACCWGGT 1 cut(s) 946
SfaNI GCATC 2 cut(s) 90, 751
SfcI CTRYAG 1 cut(s) 627
SfuI TTCGAA 1 cut(s) 503
SgrAI CRCCGGYG 1 cut(s) 65
SinI GGWCC 1 cut(s) 358
SmiMI CAYNNNNRTG 1 cut(s) 558
SmlI CTYRAG 1 cut(s) 524
SmoI CTYRAG 1 cut(s) 524
SnaBI TACGTA 1 cut(s) 933
SphI GCATGC 1 cut(s) 491
Sse9I AATT 1 cut(s) 920
SsiI CCGC 6 cut(s) 69, 96, 258, 539, 595, 825
SspMI CTAG 3 cut(s) 275, 453, 615
SstI GAGCTC 2 cut(s) 427, 549
StyD4I CCNGG 2 cut(s) 786, 946
TaiI ACGT 5 cut(s) 144, 216, 234, 873, 935
TaqI TCGA 6 cut(s) 234, 330, 351, 427, 503, 516
TasI AATT 1 cut(s) 920
Tru1I TTAA 1 cut(s) 288
Tru9I TTAA 1 cut(s) 288
TscAI CASTG 3 cut(s) 63, 82, 847
TseFI GTSAC 1 cut(s) 214
TseI GCWGC 2 cut(s) 479, 809
Tsp45I GTSAC 1 cut(s) 214
TspDTI ATGAA 3 cut(s) 190, 591, 1038
TspGWI ACGGA 2 cut(s) 154, 705
TspRI CASTG 3 cut(s) 63, 82, 847
Van91I CCANNNNNTGG 1 cut(s) 953
VpaK11BI GGWCC 1 cut(s) 358
XapI RAATTY 1 cut(s) 920
XceI RCATGY 1 cut(s) 491
XmiI GTMKAC 1 cut(s) 929
XspI CTAG 3 cut(s) 275, 453, 615
ZraI GACGTC 1 cut(s) 232
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.