RLG00000004354

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0002162
Physical Location & Seq
Forward (+)
58640660 .. 58641257
598 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000004354

Sequence Viewer

Length: 567 bp
ATGGTGTTTGCAAAGGCCAACGTTGGTCATGGGGTTGCTGGTTGGATAACAGATCCAGGCCCATGTGCTATAGGGACGATGGCAAAAAGGTCCGGCATCGGTGTATGCTCACCCGATGATAGTCGGTTGCCTGCCATTGGTGACGGGGATGGGTGGGATTCGGATTGGATGGATCTCTTTTCTCCATCTGATGGTGGTGACGGATCTTTTGGTGTGTTGGACGGCAGCGGTGCTGGTTTGGTGACTTGGTCATGGCAGTTTTCTAGCGGAGGTGGAGACTGGTCGTGGTATTCTACTCTTGTCGGCAGCAATGATCATGAGTGCTGTGACATGTTCTGGCCTGAGTCGACTTGTTTTGGAGGTTGGAGAGGCGGCTCTAGTTTTGGAAGTAGGTTGGGTGTTGATCATGGCGTGCGAGGTGGTGGCAGCAGTGATCAACATTGCGGCGGCGGCGGCAGAGGCGGAGGCGACCAAAATGGTTGTCTTGGGTCTGGATCTGGGCTTGGTTGGGAGTCCTTGTTTGGGTTTACTATTTCTGGGCTTGGTGGGCCTGTTGGGGTTGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

189

Amino Acids

18.93

Weight (kDa)

4.29

Isoelectric Point (pI)

41.18

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 191
AccI GTMKAC 1 cut(s) 347
AciI CCGC 8 cut(s) 228, 267, 372, 444, 447, 450, 453, 462
AclI AACGTT 1 cut(s) 21
AclWI GGATC 4 cut(s) 47, 180, 211, 502
AfiI CCNNNNNNNGG 3 cut(s) 137, 191, 522
AflIII ACRYGT 1 cut(s) 330
AjnI CCWGG 1 cut(s) 55
Alw26I GTCTC 1 cut(s) 270
AlwI GGATC 4 cut(s) 47, 180, 211, 502
AoxI GGCC 4 cut(s) 15, 58, 338, 548
ApeKI GCWGC 3 cut(s) 225, 306, 426
ArsI GACNNNNNNTTYG 4 cut(s) 191, 223, 466, 498
AspS9I GGNCC 3 cut(s) 59, 90, 548
AsuHPI GGTGA 4 cut(s) 102, 152, 209, 253
AvaII GGWCC 1 cut(s) 90
BbvI GCAGC 3 cut(s) 237, 318, 438
BccI CCATC 5 cut(s) 73, 143, 163, 185, 193
BceAI ACGGC 1 cut(s) 238
BciT130I CCWGG 1 cut(s) 57
BclI TGATCA 3 cut(s) 313, 403, 433
BcoDI GTCTC 1 cut(s) 270
BfaI CTAG 2 cut(s) 264, 378
BfmI CTRYAG 1 cut(s) 69
BisI GCNGC 8 cut(s) 226, 307, 373, 427, 445, 448, 451, 454
BlsI GCNGC 8 cut(s) 227, 308, 374, 428, 446, 449, 452, 455
Bme1390I CCNGG 1 cut(s) 57
Bme18I GGWCC 1 cut(s) 90
BmgT120I GGNCC 3 cut(s) 59, 90, 548
BmrFI CCNGG 1 cut(s) 57
BmsI GCATC 1 cut(s) 105
BsaXI ACNNNNNCTCC 2 cut(s) 503, 533
Bsc4I CCNNNNNNNGG 3 cut(s) 137, 191, 522
Bse1I ACTGG 1 cut(s) 284
Bse3DI GCAATG 2 cut(s) 316, 439
BseBI CCWGG 1 cut(s) 57
BseGI GGATG 2 cut(s) 154, 174
BseLI CCNNNNNNNGG 3 cut(s) 137, 191, 522
BseMI GCAATG 2 cut(s) 316, 439
BseMII CTCAG 1 cut(s) 333
BseNI ACTGG 1 cut(s) 284
BseXI GCAGC 3 cut(s) 237, 318, 438
BshFI GGCC 4 cut(s) 17, 60, 340, 550
BsiSI CCGG 1 cut(s) 93
BslFI GGGAC 1 cut(s) 88
BslI CCNNNNNNNGG 3 cut(s) 137, 191, 522
BsmAI GTCTC 1 cut(s) 270
BsmFI GGGAC 1 cut(s) 88
BsnI GGCC 4 cut(s) 17, 60, 340, 550
Bsp143I GATC 7 cut(s) 52, 172, 203, 313, 403, 433, 494
BspACI CCGC 8 cut(s) 228, 267, 372, 444, 447, 450, 453, 462
BspANI GGCC 4 cut(s) 17, 60, 340, 550
BspCNI CTCAG 1 cut(s) 334
BspHI TCATGA 1 cut(s) 316
BspPI GGATC 4 cut(s) 47, 180, 211, 502
BsrDI GCAATG 2 cut(s) 316, 439
BsrI ACTGG 1 cut(s) 284
BssMI GATC 7 cut(s) 52, 172, 203, 313, 403, 433, 494
Bst2UI CCWGG 1 cut(s) 57
BstC8I GCNNGC 2 cut(s) 132, 413
BstDEI CTNAG 1 cut(s) 342
BstF5I GGATG 2 cut(s) 154, 174
BstKTI GATC 7 cut(s) 55, 175, 206, 316, 406, 436, 497
BstMAI GTCTC 1 cut(s) 270
BstMBI GATC 7 cut(s) 52, 172, 203, 313, 403, 433, 494
BstMWI GCNNNNNNNGC 4 cut(s) 450, 453, 459, 547
BstNI CCWGG 1 cut(s) 57
BstNSI RCATGY 1 cut(s) 334
BstSCI CCNGG 1 cut(s) 55
BstSFI CTRYAG 1 cut(s) 69
BstV1I GCAGC 3 cut(s) 237, 318, 438
BstX2I RGATCY 4 cut(s) 52, 172, 203, 494
BstYI RGATCY 4 cut(s) 52, 172, 203, 494
BsuRI GGCC 4 cut(s) 17, 60, 340, 550
BtsCI GGATG 2 cut(s) 154, 174
BtsI GCAGTG 1 cut(s) 436
BtsIMutI CAGTG 1 cut(s) 436
Cac8I GCNNGC 2 cut(s) 132, 413
CciI TCATGA 1 cut(s) 316
Cfr13I GGNCC 3 cut(s) 59, 90, 548
CviAII CATG 6 cut(s) 29, 63, 252, 317, 331, 407
CviJI RGCY 7 cut(s) 17, 60, 340, 375, 502, 541, 550
CviKI_1 RGCY 7 cut(s) 17, 60, 340, 375, 502, 541, 550
DdeI CTNAG 1 cut(s) 342
DpnI GATC 7 cut(s) 54, 174, 205, 315, 405, 435, 496
DpnII GATC 7 cut(s) 52, 172, 203, 313, 403, 433, 494
EciI GGCGGA 1 cut(s) 477
Eco47I GGWCC 1 cut(s) 90
EcoRII CCWGG 1 cut(s) 55
FaeI CATG 6 cut(s) 32, 66, 255, 320, 334, 410
FaiI YATR 8 cut(s) 30, 64, 71, 106, 253, 318, 332, 408
FaqI GGGAC 1 cut(s) 88
FatI CATG 6 cut(s) 28, 62, 251, 316, 330, 406
FbaI TGATCA 3 cut(s) 313, 403, 433
FblI GTMKAC 1 cut(s) 347
Fnu4HI GCNGC 8 cut(s) 226, 307, 373, 427, 445, 448, 451, 454
FokI GGATG 2 cut(s) 161, 181
Fsp4HI GCNGC 8 cut(s) 226, 307, 373, 427, 445, 448, 451, 454
FspBI CTAG 2 cut(s) 264, 378
GluI GCNGC 8 cut(s) 226, 307, 373, 427, 445, 448, 451, 454
HaeIII GGCC 4 cut(s) 17, 60, 340, 550
HapII CCGG 1 cut(s) 93
Hin1II CATG 6 cut(s) 32, 66, 255, 320, 334, 410
HincII GTYRAC 1 cut(s) 348
HindII GTYRAC 1 cut(s) 348
HinfI GANTC 3 cut(s) 158, 344, 512
HpaII CCGG 1 cut(s) 93
HphI GGTGA 4 cut(s) 102, 152, 209, 253
Hpy166II GTNNAC 2 cut(s) 348, 528
Hpy188I TCNGA 2 cut(s) 163, 190
Hpy188III TCNNGA 2 cut(s) 317, 492
Hpy8I GTNNAC 2 cut(s) 348, 528
HpyCH4IV ACGT 1 cut(s) 21
HpyCH4V TGCA 1 cut(s) 11
HpyF10VI GCNNNNNNNGC 4 cut(s) 450, 453, 459, 547
HpyF3I CTNAG 1 cut(s) 342
HpySE526I ACGT 1 cut(s) 21
Hsp92II CATG 6 cut(s) 32, 66, 255, 320, 334, 410
Ksp22I TGATCA 3 cut(s) 313, 403, 433
Kzo9I GATC 7 cut(s) 52, 172, 203, 313, 403, 433, 494
Lsp1109I GCAGC 3 cut(s) 237, 318, 438
LweI GCATC 1 cut(s) 105
MaeI CTAG 2 cut(s) 264, 378
MaeII ACGT 1 cut(s) 21
MaeIII GTNAC 4 cut(s) 140, 197, 241, 326
MalI GATC 7 cut(s) 54, 174, 205, 315, 405, 435, 496
MboI GATC 7 cut(s) 52, 172, 203, 313, 403, 433, 494
MflI RGATCY 4 cut(s) 52, 172, 203, 494
MlyI GAGTC 2 cut(s) 353, 521
MmeI TCCRAC 3 cut(s) 23, 198, 344
MnlI CCTC 6 cut(s) 263, 353, 362, 410, 452, 458
MseI TTAA 1 cut(s) 565
MspA1I CMGCKG 1 cut(s) 228
MspI CCGG 1 cut(s) 93
MspR9I CCNGG 1 cut(s) 57
MvaI CCWGG 1 cut(s) 57
MwoI GCNNNNNNNGC 4 cut(s) 450, 453, 459, 547
NdeII GATC 7 cut(s) 52, 172, 203, 313, 403, 433, 494
NlaIII CATG 6 cut(s) 32, 66, 255, 320, 334, 410
NmuCI GTSAC 4 cut(s) 140, 197, 241, 326
NspI RCATGY 1 cut(s) 334
PagI TCATGA 1 cut(s) 316
PciI ACATGT 1 cut(s) 330
PfeI GAWTC 1 cut(s) 158
PflFI GACNNNGTC 1 cut(s) 247
PflMI CCANNNNNTGG 1 cut(s) 191
PkrI GCNGC 8 cut(s) 227, 308, 374, 428, 446, 449, 452, 455
PleI GAGTC 2 cut(s) 352, 520
PpsI GAGTC 2 cut(s) 352, 520
PscI ACATGT 1 cut(s) 330
Psp1406I AACGTT 1 cut(s) 21
Psp6I CCWGG 1 cut(s) 55
PspGI CCWGG 1 cut(s) 55
PspPI GGNCC 3 cut(s) 59, 90, 548
PsuI RGATCY 4 cut(s) 52, 172, 203, 494
PsyI GACNNNGTC 1 cut(s) 247
SalI GTCGAC 1 cut(s) 346
SaqAI TTAA 1 cut(s) 565
SatI GCNGC 8 cut(s) 226, 307, 373, 427, 445, 448, 451, 454
Sau3AI GATC 7 cut(s) 52, 172, 203, 313, 403, 433, 494
Sau96I GGNCC 3 cut(s) 59, 90, 548
SchI GAGTC 2 cut(s) 353, 521
ScrFI CCNGG 1 cut(s) 57
SetI ASST 6 cut(s) 24, 92, 274, 364, 395, 421
SfaNI GCATC 1 cut(s) 105
SfcI CTRYAG 1 cut(s) 69
SinI GGWCC 1 cut(s) 90
SsiI CCGC 8 cut(s) 228, 267, 372, 444, 447, 450, 453, 462
SspMI CTAG 2 cut(s) 264, 378
StyD4I CCNGG 1 cut(s) 55
TaiI ACGT 1 cut(s) 24
TaqI TCGA 1 cut(s) 347
TauI GCSGC 5 cut(s) 375, 447, 450, 453, 456
TfiI GAWTC 1 cut(s) 158
Tru1I TTAA 1 cut(s) 565
Tru9I TTAA 1 cut(s) 565
TscAI CASTG 1 cut(s) 436
TseFI GTSAC 4 cut(s) 140, 197, 241, 326
TseI GCWGC 3 cut(s) 225, 306, 426
Tsp45I GTSAC 4 cut(s) 140, 197, 241, 326
TspGWI ACGGA 1 cut(s) 216
TspRI CASTG 1 cut(s) 436
Tth111I GACNNNGTC 1 cut(s) 247
Van91I CCANNNNNTGG 1 cut(s) 191
VpaK11BI GGWCC 1 cut(s) 90
XceI RCATGY 1 cut(s) 334
XmiI GTMKAC 1 cut(s) 347
XspI CTAG 2 cut(s) 264, 378
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.