Rh2AG289100

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0002162
Physical Location & Seq
Reverse (-)
35476017 .. 35476514
498 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG289100.1

Sequence Viewer

Length: 498 bp
ATGTGGAGCTGTGTTGTGGAGGGAGGCTCTTCTACTAGAGGCGGTGGTGAGTCTAGACTGGGCTTCCTTCTCCACGATGTCGCTGTGGTAGGTGACCCTACTACTGAGGAAGAGGATGGAGCTATATCCATGGGTGAATGGGCCCCGGCACCGAGTTTTGGCTTTTCAATCGGTGGTCAAGGTCTTGTCCCTCTTGTAGGCTATGATCTAATACGGTTCTGCACTTCTCAGTACAAGGATGATGGTGGCTATGGTGTTCTCGCTGGCGGCAGCCTTGTTGGCTCCAGCGGCGGTAGTGGGTTGGAGGCCACCAGGGTTGGAATTGTTCTTGGTGGCTTTGGGCTTTTGCCAGGGTTTCTCATAATTGGGCCTATTGGGTTAACGTGGGCTGGCTTAACTTGGGCTCATAGCATGATTAGGGTTATTGTTTTTATTTTTGCTTTGGGTTATTATCATTTTTTAAGGAATGATGATGGCTCTATTATTTTCTTTTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

165

Amino Acids

17.22

Weight (kDa)

4.55

Isoelectric Point (pI)

28.72

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 148
AciI CCGC 4 cut(s) 42, 267, 288, 291
AfaI GTAC 1 cut(s) 233
AfiI CCNNNNNNNGG 2 cut(s) 158, 197
AgsI TTSAA 1 cut(s) 168
AjnI CCWGG 2 cut(s) 311, 349
AluBI AGCT 2 cut(s) 9, 122
AluI AGCT 2 cut(s) 9, 122
AoxI GGCC 3 cut(s) 141, 306, 368
ApaI GGGCCC 1 cut(s) 145
ApeKI GCWGC 1 cut(s) 270
AspS9I GGNCC 3 cut(s) 141, 142, 368
AsuC2I CCSGG 1 cut(s) 146
AsuHPI GGTGA 3 cut(s) 59, 104, 146
BaeGI GKGCMC 1 cut(s) 145
BanI GGYRCC 1 cut(s) 148
BanII GRGCYC 2 cut(s) 145, 406
BbvI GCAGC 1 cut(s) 282
BccI CCATC 3 cut(s) 110, 236, 467
BciT130I CCWGG 2 cut(s) 313, 351
BcnI CCSGG 1 cut(s) 146
BfaI CTAG 2 cut(s) 36, 54
BglI GCCNNNNNGGC 1 cut(s) 279
BisI GCNGC 3 cut(s) 268, 271, 289
BlsI GCNGC 3 cut(s) 269, 272, 290
Bme1390I CCNGG 3 cut(s) 146, 313, 351
BmgT120I GGNCC 3 cut(s) 141, 142, 368
BmiI GGNNCC 4 cut(s) 143, 144, 150, 283
BmrFI CCNGG 3 cut(s) 146, 313, 351
BmrI ACTGGG 1 cut(s) 68
BmuI ACTGGG 1 cut(s) 68
BplI GAGNNNNNCTC 2 cut(s) 11, 43
BpmI CTGGAG 1 cut(s) 268
BpuMI CCSGG 1 cut(s) 146
BsaJI CCNNGG 4 cut(s) 129, 144, 312, 350
Bsc4I CCNNNNNNNGG 2 cut(s) 158, 197
Bse1I ACTGG 1 cut(s) 63
BseBI CCWGG 2 cut(s) 313, 351
BseDI CCNNGG 4 cut(s) 129, 144, 312, 350
BseGI GGATG 2 cut(s) 121, 244
BseLI CCNNNNNNNGG 2 cut(s) 158, 197
BseMII CTCAG 2 cut(s) 96, 242
BseNI ACTGG 1 cut(s) 63
BseSI GKGCMC 1 cut(s) 145
BseXI GCAGC 1 cut(s) 282
BsgI GTGCAG 1 cut(s) 205
BshFI GGCC 3 cut(s) 143, 308, 370
BshNI GGYRCC 1 cut(s) 148
BsiSI CCGG 1 cut(s) 146
BslFI GGGAC 1 cut(s) 173
BslI CCNNNNNNNGG 2 cut(s) 158, 197
BsmFI GGGAC 1 cut(s) 173
BsnI GGCC 3 cut(s) 143, 308, 370
Bsp120I GGGCCC 1 cut(s) 141
Bsp1286I GDGCHC 2 cut(s) 145, 406
Bsp143I GATC 1 cut(s) 205
Bsp19I CCATGG 1 cut(s) 129
BspACI CCGC 4 cut(s) 42, 267, 288, 291
BspANI GGCC 3 cut(s) 143, 308, 370
BspCNI CTCAG 2 cut(s) 97, 241
BspLI GGNNCC 4 cut(s) 143, 144, 150, 283
BspQI GCTCTTC 1 cut(s) 34
BspT107I GGYRCC 1 cut(s) 148
BsrI ACTGG 1 cut(s) 63
BssECI CCNNGG 4 cut(s) 129, 144, 312, 350
BssMI GATC 1 cut(s) 205
BssT1I CCWWGG 1 cut(s) 129
Bst2UI CCWGG 2 cut(s) 313, 351
Bst4CI ACNGT 1 cut(s) 216
Bst6I CTCTTC 2 cut(s) 34, 105
BstC8I GCNNGC 2 cut(s) 265, 391
BstDEI CTNAG 2 cut(s) 105, 228
BstDSI CCRYGG 1 cut(s) 129
BstEII GGTNACC 1 cut(s) 92
BstENI CCTNNNNNAGG 1 cut(s) 195
BstF5I GGATG 2 cut(s) 121, 244
BstKTI GATC 1 cut(s) 208
BstMBI GATC 1 cut(s) 205
BstMWI GCNNNNNNNGC 2 cut(s) 279, 288
BstNI CCWGG 2 cut(s) 313, 351
BstPI GGTNACC 1 cut(s) 92
BstSCI CCNGG 3 cut(s) 144, 311, 349
BstSLI GKGCMC 1 cut(s) 145
BstV1I GCAGC 1 cut(s) 282
BsuRI GGCC 3 cut(s) 143, 308, 370
BtgI CCRYGG 1 cut(s) 129
BtsCI GGATG 2 cut(s) 121, 244
Cac8I GCNNGC 2 cut(s) 265, 391
Cfr13I GGNCC 3 cut(s) 141, 142, 368
Csp6I GTAC 1 cut(s) 232
CspCI CAANNNNNGTGG 2 cut(s) 298, 333
CviAII CATG 2 cut(s) 130, 412
CviQI GTAC 1 cut(s) 232
DdeI CTNAG 2 cut(s) 105, 228
DpnI GATC 1 cut(s) 207
DpnII GATC 1 cut(s) 205
Eam1104I CTCTTC 2 cut(s) 34, 105
EarI CTCTTC 2 cut(s) 34, 105
Eco130I CCWWGG 1 cut(s) 129
Eco24I GRGCYC 2 cut(s) 145, 406
Eco91I GGTNACC 1 cut(s) 92
EcoNI CCTNNNNNAGG 1 cut(s) 195
EcoO109I RGGNCCY 1 cut(s) 142
EcoO65I GGTNACC 1 cut(s) 92
EcoRII CCWGG 2 cut(s) 311, 349
EcoT14I CCWWGG 1 cut(s) 129
EcoT38I GRGCYC 2 cut(s) 145, 406
ErhI CCWWGG 1 cut(s) 129
FaeI CATG 2 cut(s) 133, 415
FaiI YATR 7 cut(s) 125, 131, 204, 252, 362, 408, 413
FaqI GGGAC 1 cut(s) 173
FatI CATG 2 cut(s) 129, 411
Fnu4HI GCNGC 3 cut(s) 268, 271, 289
FokI GGATG 2 cut(s) 128, 251
FriOI GRGCYC 2 cut(s) 145, 406
Fsp4HI GCNGC 3 cut(s) 268, 271, 289
FspBI CTAG 2 cut(s) 36, 54
GluI GCNGC 3 cut(s) 268, 271, 289
GsuI CTGGAG 1 cut(s) 268
HaeIII GGCC 3 cut(s) 143, 308, 370
HapII CCGG 1 cut(s) 146
Hin1II CATG 2 cut(s) 133, 415
HincII GTYRAC 1 cut(s) 381
HindII GTYRAC 1 cut(s) 381
HinfI GANTC 1 cut(s) 50
HpaI GTTAAC 1 cut(s) 381
HpaII CCGG 1 cut(s) 146
HphI GGTGA 3 cut(s) 59, 104, 146
Hpy166II GTNNAC 1 cut(s) 381
Hpy188III TCNNGA 1 cut(s) 54
Hpy8I GTNNAC 1 cut(s) 381
HpyAV CCTTC 1 cut(s) 77
HpyCH4III ACNGT 1 cut(s) 216
HpyCH4IV ACGT 1 cut(s) 383
HpyCH4V TGCA 1 cut(s) 222
HpyF10VI GCNNNNNNNGC 2 cut(s) 279, 288
HpyF3I CTNAG 2 cut(s) 105, 228
HpySE526I ACGT 1 cut(s) 383
Hsp92II CATG 2 cut(s) 133, 415
KspAI GTTAAC 1 cut(s) 381
Kzo9I GATC 1 cut(s) 205
LguI GCTCTTC 1 cut(s) 34
LmnI GCTCC 3 cut(s) 6, 119, 287
LpnPI CCDG 9 cut(s) 44, 159, 249, 298, 298, 325, 336, 363, 375
Lsp1109I GCAGC 1 cut(s) 282
MaeI CTAG 2 cut(s) 36, 54
MaeII ACGT 1 cut(s) 383
MaeIII GTNAC 1 cut(s) 92
MalI GATC 1 cut(s) 207
MboI GATC 1 cut(s) 205
MboII GAAGA 2 cut(s) 21, 122
MhlI GDGCHC 2 cut(s) 145, 406
MluCI AATT 2 cut(s) 321, 363
MlyI GAGTC 1 cut(s) 59
MmeI TCCRAC 2 cut(s) 282, 298
MnlI CCTC 7 cut(s) 13, 17, 32, 100, 106, 201, 298
MseI TTAA 3 cut(s) 380, 395, 461
MspA1I CMGCKG 1 cut(s) 288
MspI CCGG 1 cut(s) 146
MspR9I CCNGG 3 cut(s) 146, 313, 351
MvaI CCWGG 2 cut(s) 313, 351
MwoI GCNNNNNNNGC 2 cut(s) 279, 288
NciI CCSGG 1 cut(s) 146
NcoI CCATGG 1 cut(s) 129
NdeII GATC 1 cut(s) 205
NlaIII CATG 2 cut(s) 133, 415
NlaIV GGNNCC 4 cut(s) 143, 144, 150, 283
NmuCI GTSAC 1 cut(s) 92
PciSI GCTCTTC 1 cut(s) 34
PkrI GCNGC 3 cut(s) 269, 272, 290
PleI GAGTC 1 cut(s) 58
PpsI GAGTC 1 cut(s) 58
Psp6I CCWGG 2 cut(s) 311, 349
PspEI GGTNACC 1 cut(s) 92
PspGI CCWGG 2 cut(s) 311, 349
PspN4I GGNNCC 4 cut(s) 143, 144, 150, 283
PspOMI GGGCCC 1 cut(s) 141
PspPI GGNCC 3 cut(s) 141, 142, 368
RsaI GTAC 1 cut(s) 233
RsaNI GTAC 1 cut(s) 232
SapI GCTCTTC 1 cut(s) 34
SaqAI TTAA 3 cut(s) 380, 395, 461
SatI GCNGC 3 cut(s) 268, 271, 289
Sau3AI GATC 1 cut(s) 205
Sau96I GGNCC 3 cut(s) 141, 142, 368
SchI GAGTC 1 cut(s) 59
ScrFI CCNGG 3 cut(s) 146, 313, 351
SduI GDGCHC 2 cut(s) 145, 406
SetI ASST 5 cut(s) 11, 94, 124, 184, 386
Sse9I AATT 2 cut(s) 321, 363
SsiI CCGC 4 cut(s) 42, 267, 288, 291
SspMI CTAG 2 cut(s) 36, 54
StyD4I CCNGG 3 cut(s) 144, 311, 349
StyI CCWWGG 1 cut(s) 129
TaaI ACNGT 1 cut(s) 216
TaiI ACGT 1 cut(s) 386
TasI AATT 2 cut(s) 321, 363
TatI WGTACW 1 cut(s) 231
TauI GCSGC 2 cut(s) 270, 291
Tru1I TTAA 3 cut(s) 380, 395, 461
Tru9I TTAA 3 cut(s) 380, 395, 461
TseFI GTSAC 1 cut(s) 92
TseI GCWGC 1 cut(s) 270
Tsp45I GTSAC 1 cut(s) 92
XagI CCTNNNNNAGG 1 cut(s) 195
XbaI TCTAGA 1 cut(s) 53
XspI CTAG 2 cut(s) 36, 54
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.