RLG00000005258

short-chain dehydrogenase reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0002162
Physical Location & Seq
Reverse (-)
66259857 .. 66260325
469 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000005258

Sequence Viewer

Length: 438 bp
ATGACAGAACTCAACAAGAAGCTTCAAGACAAGGTGGCCATAGTCACCGGTGGCGCCAGCGGCATTGGCGAAGCCACCGCGCCTCATTTTGCATTGCATGGTGCATGTGCGGTGGTCATCGCCGACGTTCAGGACCAAAAGCGCTTCGATCGGCCCTCACTACGAGGATCAAGTGAAGAGCCTGGTCGAATCAACGGTCCAGATGCACGGCCGCCTGGACATCATGTTCAGCAACGCCGGAATATTCTGCTGTCCAAGCAGACGGTGCTGGAGTTTGACTTATCCAAGTACGACAAGCTCATGGCGGTCAATGTACGTGGGATGGAGGCGTGTGTGAAGCACGCTGCGCGGGCCATGGTGGCAGGTGGAGTTAAGGGGAGCGTGATATGCACGGCGAGCGTTGTAGCGACTACTGGATTAGAGTGGTTCACAGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
adh_short_C2

Protein Analysis

146

Amino Acids

15.48

Weight (kDa)

8.88

Isoelectric Point (pI)

45.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short PF00106 86 - 142 5.6e-06 short chain dehydrogenase
adh_short_C2 PF13561 86 - 139 3.8e-06 Enoyl-(Acyl carrier protein) reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 353
Acc36I ACCTGC 1 cut(s) 353
AccB1I GGYRCC 1 cut(s) 53
AccII CGCG 2 cut(s) 80, 349
AciI CCGC 6 cut(s) 60, 78, 110, 212, 305, 349
AclWI GGATC 1 cut(s) 175
AcoI YGGCCR 2 cut(s) 36, 209
AcyI GRCGYC 1 cut(s) 54
AfaI GTAC 2 cut(s) 290, 315
AfeI AGCGCT 1 cut(s) 143
AgeI ACCGGT 1 cut(s) 47
AgsI TTSAA 1 cut(s) 26
AjnI CCWGG 2 cut(s) 181, 214
AloI GAACNNNNNNTCC 2 cut(s) 210, 242
AluBI AGCT 2 cut(s) 22, 298
AluI AGCT 2 cut(s) 22, 298
AlwI GGATC 1 cut(s) 175
Aor51HI AGCGCT 1 cut(s) 143
AoxI GGCC 4 cut(s) 36, 152, 209, 351
ApeKI GCWGC 1 cut(s) 344
ArsI GACNNNNNNTTYG 2 cut(s) 181, 213
AsiGI ACCGGT 1 cut(s) 47
AspLEI GCGC 4 cut(s) 56, 82, 144, 349
AspS9I GGNCC 4 cut(s) 133, 153, 197, 351
AsuHPI GGTGA 1 cut(s) 37
AvaII GGWCC 2 cut(s) 133, 197
BalI TGGCCA 1 cut(s) 38
BanI GGYRCC 1 cut(s) 53
BbvI GCAGC 1 cut(s) 331
BccI CCATC 1 cut(s) 316
BceAI ACGGC 2 cut(s) 224, 408
BciT130I CCWGG 2 cut(s) 183, 216
BfaI CTAG 1 cut(s) 436
BfoI RGCGCY 2 cut(s) 57, 145
BfuAI ACCTGC 1 cut(s) 353
BglI GCCNNNNNGGC 1 cut(s) 359
BisI GCNGC 3 cut(s) 61, 212, 345
BlsI GCNGC 3 cut(s) 62, 213, 346
Bme1390I CCNGG 2 cut(s) 183, 216
Bme18I GGWCC 2 cut(s) 133, 197
BmgT120I GGNCC 4 cut(s) 133, 153, 197, 351
BmiI GGNNCC 1 cut(s) 55
BmrFI CCNGG 2 cut(s) 183, 216
BmsI GCATC 1 cut(s) 193
BpmI CTGGAG 1 cut(s) 290
BsaAI YACGTR 1 cut(s) 317
BsaHI GRCGYC 1 cut(s) 54
BsaJI CCNNGG 1 cut(s) 354
BsaWI WCCGGW 1 cut(s) 47
Bse118I RCCGGY 1 cut(s) 47
Bse1I ACTGG 1 cut(s) 418
Bse3DI GCAATG 1 cut(s) 92
BseBI CCWGG 2 cut(s) 183, 216
BseDI CCNNGG 1 cut(s) 354
BseGI GGATG 1 cut(s) 327
BseMI GCAATG 1 cut(s) 92
BseNI ACTGG 1 cut(s) 418
BseX3I CGGCCG 1 cut(s) 209
BseXI GCAGC 1 cut(s) 331
Bsh1236I CGCG 2 cut(s) 80, 349
Bsh1285I CGRYCG 2 cut(s) 151, 212
BshFI GGCC 4 cut(s) 38, 154, 211, 353
BshNI GGYRCC 1 cut(s) 53
BshTI ACCGGT 1 cut(s) 47
BsiEI CGRYCG 2 cut(s) 151, 212
BsiSI CCGG 2 cut(s) 48, 238
BsnI GGCC 4 cut(s) 38, 154, 211, 353
Bsp143I GATC 2 cut(s) 148, 167
Bsp19I CCATGG 1 cut(s) 354
BspACI CCGC 6 cut(s) 60, 78, 110, 212, 305, 349
BspANI GGCC 4 cut(s) 38, 154, 211, 353
BspFNI CGCG 2 cut(s) 80, 349
BspLI GGNNCC 1 cut(s) 55
BspMI ACCTGC 1 cut(s) 353
BspPI GGATC 1 cut(s) 175
BspQI GCTCTTC 1 cut(s) 171
BspT107I GGYRCC 1 cut(s) 53
BsrDI GCAATG 1 cut(s) 92
BsrFI RCCGGY 1 cut(s) 47
BsrI ACTGG 1 cut(s) 418
BssAI RCCGGY 1 cut(s) 47
BssECI CCNNGG 1 cut(s) 354
BssMI GATC 2 cut(s) 148, 167
BssNI GRCGYC 1 cut(s) 54
BssT1I CCWWGG 1 cut(s) 354
Bst2UI CCWGG 2 cut(s) 183, 216
Bst4CI ACNGT 2 cut(s) 197, 265
Bst6I CTCTTC 1 cut(s) 171
BstACI GRCGYC 1 cut(s) 54
BstAPI GCANNNNNTGC 1 cut(s) 265
BstBAI YACGTR 1 cut(s) 317
BstC8I GCNNGC 4 cut(s) 58, 342, 351, 397
BstDSI CCRYGG 1 cut(s) 354
BstF5I GGATG 1 cut(s) 327
BstFNI CGCG 2 cut(s) 80, 349
BstH2I RGCGCY 2 cut(s) 57, 145
BstHHI GCGC 4 cut(s) 56, 82, 144, 349
BstKTI GATC 2 cut(s) 151, 170
BstMBI GATC 2 cut(s) 148, 167
BstMCI CGRYCG 2 cut(s) 151, 212
BstMWI GCNNNNNNNGC 9 cut(s) 60, 66, 256, 265, 346, 350, 359, 387, 396
BstNI CCWGG 2 cut(s) 183, 216
BstNSI RCATGY 1 cut(s) 108
BstSCI CCNGG 2 cut(s) 181, 214
BstUI CGCG 2 cut(s) 80, 349
BstV1I GCAGC 1 cut(s) 331
BstZI CGGCCG 1 cut(s) 209
BsuRI GGCC 4 cut(s) 38, 154, 211, 353
BtgI CCRYGG 1 cut(s) 354
BtgZI GCGATG 1 cut(s) 103
BtsCI GGATG 1 cut(s) 327
BveI ACCTGC 1 cut(s) 353
Cac8I GCNNGC 4 cut(s) 58, 342, 351, 397
CfoI GCGC 4 cut(s) 56, 82, 144, 349
Cfr10I RCCGGY 1 cut(s) 47
Cfr13I GGNCC 4 cut(s) 133, 153, 197, 351
Csp6I GTAC 2 cut(s) 289, 314
CspAI ACCGGT 1 cut(s) 47
CspCI CAANNNNNGTGG 2 cut(s) 298, 333
CviAII CATG 5 cut(s) 98, 105, 224, 301, 355
CviJI RGCY 8 cut(s) 22, 38, 74, 154, 181, 211, 298, 353
CviKI_1 RGCY 8 cut(s) 22, 38, 74, 154, 181, 211, 298, 353
CviQI GTAC 2 cut(s) 289, 314
DinI GGCGCC 1 cut(s) 55
DpnI GATC 2 cut(s) 150, 169
DpnII GATC 2 cut(s) 148, 167
EaeI YGGCCR 2 cut(s) 36, 209
EagI CGGCCG 1 cut(s) 209
Eam1104I CTCTTC 1 cut(s) 171
EarI CTCTTC 1 cut(s) 171
EclXI CGGCCG 1 cut(s) 209
Eco130I CCWWGG 1 cut(s) 354
Eco47I GGWCC 2 cut(s) 133, 197
Eco47III AGCGCT 1 cut(s) 143
Eco52I CGGCCG 1 cut(s) 209
EcoRII CCWGG 2 cut(s) 181, 214
EcoT14I CCWWGG 1 cut(s) 354
EgeI GGCGCC 1 cut(s) 55
EheI GGCGCC 1 cut(s) 55
ErhI CCWWGG 1 cut(s) 354
FaeI CATG 5 cut(s) 101, 108, 227, 304, 358
FaiI YATR 7 cut(s) 41, 99, 106, 225, 302, 356, 388
FatI CATG 5 cut(s) 97, 104, 223, 300, 354
FauI CCCGC 1 cut(s) 342
Fnu4HI GCNGC 3 cut(s) 61, 212, 345
FokI GGATG 1 cut(s) 334
Fsp4HI GCNGC 3 cut(s) 61, 212, 345
FspBI CTAG 1 cut(s) 436
GlaI GCGC 4 cut(s) 55, 81, 143, 348
GluI GCNGC 3 cut(s) 61, 212, 345
GsuI CTGGAG 1 cut(s) 290
HaeII RGCGCY 2 cut(s) 57, 145
HaeIII GGCC 4 cut(s) 38, 154, 211, 353
HapII CCGG 2 cut(s) 48, 238
HhaI GCGC 4 cut(s) 56, 82, 144, 349
Hin1I GRCGYC 1 cut(s) 54
Hin1II CATG 5 cut(s) 101, 108, 227, 304, 358
Hin6I GCGC 4 cut(s) 54, 80, 142, 347
HinP1I GCGC 4 cut(s) 54, 80, 142, 347
HindIII AAGCTT 1 cut(s) 20
HinfI GANTC 1 cut(s) 189
HpaII CCGG 2 cut(s) 48, 238
HphI GGTGA 1 cut(s) 37
Hpy166II GTNNAC 1 cut(s) 429
Hpy188III TCNNGA 3 cut(s) 26, 131, 200
Hpy8I GTNNAC 1 cut(s) 429
Hpy99I CGWCG 1 cut(s) 128
HpyCH4III ACNGT 2 cut(s) 197, 265
HpyCH4IV ACGT 2 cut(s) 126, 316
HpyCH4V TGCA 5 cut(s) 92, 97, 104, 206, 390
HpyF10VI GCNNNNNNNGC 9 cut(s) 60, 66, 256, 265, 346, 350, 359, 387, 396
HpySE526I ACGT 2 cut(s) 126, 316
Hsp92I GRCGYC 1 cut(s) 54
Hsp92II CATG 5 cut(s) 101, 108, 227, 304, 358
HspAI GCGC 4 cut(s) 54, 80, 142, 347
KasI GGCGCC 1 cut(s) 53
Kzo9I GATC 2 cut(s) 148, 167
LguI GCTCTTC 1 cut(s) 171
LmnI GCTCC 1 cut(s) 378
Lsp1109I GCAGC 1 cut(s) 331
LweI GCATC 1 cut(s) 193
MaeI CTAG 1 cut(s) 436
MaeII ACGT 2 cut(s) 126, 316
MaeIII GTNAC 1 cut(s) 43
MalI GATC 2 cut(s) 150, 169
MboI GATC 2 cut(s) 148, 167
MboII GAAGA 1 cut(s) 188
MlsI TGGCCA 1 cut(s) 38
MluNI TGGCCA 1 cut(s) 38
Mly113I GGCGCC 1 cut(s) 54
MnlI CCTC 4 cut(s) 93, 158, 166, 319
Mox20I TGGCCA 1 cut(s) 38
MscI TGGCCA 1 cut(s) 38
MseI TTAA 1 cut(s) 372
Msp20I TGGCCA 1 cut(s) 38
MspA1I CMGCKG 1 cut(s) 60
MspI CCGG 2 cut(s) 48, 238
MspR9I CCNGG 2 cut(s) 183, 216
MvaI CCWGG 2 cut(s) 183, 216
MvnI CGCG 2 cut(s) 80, 349
MwoI GCNNNNNNNGC 9 cut(s) 60, 66, 256, 265, 346, 350, 359, 387, 396
NarI GGCGCC 1 cut(s) 54
NcoI CCATGG 1 cut(s) 354
NdeII GATC 2 cut(s) 148, 167
NlaIII CATG 5 cut(s) 101, 108, 227, 304, 358
NlaIV GGNNCC 1 cut(s) 55
NmuCI GTSAC 1 cut(s) 43
NspI RCATGY 1 cut(s) 108
PaqCI CACCTGC 1 cut(s) 353
PciSI GCTCTTC 1 cut(s) 171
PfeI GAWTC 1 cut(s) 189
PinAI ACCGGT 1 cut(s) 47
PkrI GCNGC 3 cut(s) 62, 213, 346
Ple19I CGATCG 1 cut(s) 151
PluTI GGCGCC 1 cut(s) 57
Ppu21I YACGTR 1 cut(s) 317
Psp6I CCWGG 2 cut(s) 181, 214
PspGI CCWGG 2 cut(s) 181, 214
PspN4I GGNNCC 1 cut(s) 55
PspPI GGNCC 4 cut(s) 133, 153, 197, 351
PvuI CGATCG 1 cut(s) 151
RsaI GTAC 2 cut(s) 290, 315
RsaNI GTAC 2 cut(s) 289, 314
SapI GCTCTTC 1 cut(s) 171
SaqAI TTAA 1 cut(s) 372
SatI GCNGC 3 cut(s) 61, 212, 345
Sau3AI GATC 2 cut(s) 148, 167
Sau96I GGNCC 4 cut(s) 133, 153, 197, 351
ScrFI CCNGG 2 cut(s) 183, 216
SetI ASST 6 cut(s) 24, 36, 129, 300, 319, 367
SfaNI GCATC 1 cut(s) 193
SfoI GGCGCC 1 cut(s) 55
SgrAI CRCCGGYG 1 cut(s) 47
SinI GGWCC 2 cut(s) 133, 197
SsiI CCGC 6 cut(s) 60, 78, 110, 212, 305, 349
SspDI GGCGCC 1 cut(s) 53
SspI AATATT 1 cut(s) 244
SspMI CTAG 1 cut(s) 436
StyD4I CCNGG 2 cut(s) 181, 214
StyI CCWWGG 1 cut(s) 354
TaaI ACNGT 2 cut(s) 197, 265
TaiI ACGT 2 cut(s) 129, 319
TaqI TCGA 2 cut(s) 147, 187
TauI GCSGC 2 cut(s) 63, 214
TfiI GAWTC 1 cut(s) 189
Tru1I TTAA 1 cut(s) 372
Tru9I TTAA 1 cut(s) 372
TseFI GTSAC 1 cut(s) 43
TseI GCWGC 1 cut(s) 344
Tsp45I GTSAC 1 cut(s) 43
VpaK11BI GGWCC 2 cut(s) 133, 197
XceI RCATGY 1 cut(s) 108
XspI CTAG 1 cut(s) 436
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.