Rh3AG046600

Cysteine synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0002162
Physical Location & Seq
Forward (+)
3254944 .. 3261908
6965 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG046600.1

Sequence Viewer

Length: 696 bp
ATGGAGGGTGACCCTACTACTGAGGAAGACGATGGAGCTATATCCATGGGTGAATGGGCGCCGGCGCCGAGTTTTGGCTTTTCAATTGGTGGTCAAGGTCTTGTCCCTCTCGCAGGCTATGATCTAATACGGTTTTGCACTCCTCAGTACAAGGATGATGGTGGCTATAGTGTTCTCGCTGGCGACAACCTTGTTGGCTACGGCGGCGGTAGTGGGTTGGAGGCCACCAAGGTTGCAATTGTTCTTGGTGGCCTTGGGCTTTTGCTAGGGTTTCTCATAATTGGGCATATTGGGTTAGCGTGGACTGGTTTAACTTGGGCTCATAGCATGATTAGGGTGTACGGTGTAGAACCAGTTGAGAGTGCAGTCTTGAATGGTGGTCCACCTGGAAAGCATCTGATCCAAGGAATCGGTGCTGGCTTCATTCCTCAATTTCTTGATGTTAGCCTGCCAGACGAAGTTATTCCAGTATCTGGTGAGGAATCTATTGAGACTGCTAAGCTACTTACCTTAAAAGAAGGTCTTTTGGTGGGAATCTCATCTGGTGCTGCAACAGCGCGCAGCAGCAATAAAGTTGGCAAAAAAGCAAGAAAATGCCGGAAAACTAATAGTTGTCGTGTTCCCCAGTTTAGGAGAGCGGTATATATCTGTCTACTGCACTCTTTGATACCATTAGGCATGAGGCAGAAAACATGA

Protein Analysis

231

Amino Acids

24.18

Weight (kDa)

6.42

Isoelectric Point (pI)

31.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PALP PF00291 101 - 188 1.2e-09 Pyridoxal-phosphate dependent enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 58, 64
AccB7I CCANNNNNTGG 1 cut(s) 473
AccBSI CCGCTC 1 cut(s) 638
AccI GTMKAC 1 cut(s) 652
AccII CGCG 1 cut(s) 559
AciI CCGC 3 cut(s) 204, 207, 638
AclWI GGATC 1 cut(s) 394
AcyI GRCGYC 2 cut(s) 59, 65
AfaI GTAC 2 cut(s) 149, 341
AfiI CCNNNNNNNGG 4 cut(s) 74, 113, 473, 630
AgsI TTSAA 2 cut(s) 84, 373
AjnI CCWGG 1 cut(s) 385
AluBI AGCT 2 cut(s) 38, 502
AluI AGCT 2 cut(s) 38, 502
Alw26I GTCTC 1 cut(s) 485
AlwI GGATC 1 cut(s) 394
AlwNI CAGNNNCTG 1 cut(s) 473
AoxI GGCC 2 cut(s) 222, 250
ApeKI GCWGC 3 cut(s) 548, 561, 564
Asp700I GAANNNNTTC 1 cut(s) 462
AspLEI GCGC 4 cut(s) 61, 67, 559, 561
AspS9I GGNCC 1 cut(s) 380
AsuHPI GGTGA 3 cut(s) 20, 62, 488
AvaII GGWCC 1 cut(s) 380
BanI GGYRCC 2 cut(s) 58, 64
BanII GRGCYC 1 cut(s) 322
BbsI GAAGAC 1 cut(s) 33
BbvI GCAGC 3 cut(s) 535, 573, 576
BccI CCATC 2 cut(s) 26, 152
BceAI ACGGC 1 cut(s) 217
BciT130I CCWGG 1 cut(s) 387
BcoDI GTCTC 1 cut(s) 485
BfaI CTAG 1 cut(s) 266
BfmI CTRYAG 1 cut(s) 166
BfoI RGCGCY 2 cut(s) 62, 68
BisI GCNGC 4 cut(s) 205, 549, 562, 565
BlpI GCTNAGC 1 cut(s) 498
BlsI GCNGC 4 cut(s) 206, 550, 563, 566
Bme1390I CCNGG 1 cut(s) 387
Bme18I GGWCC 1 cut(s) 380
BmgT120I GGNCC 1 cut(s) 380
BmiI GGNNCC 2 cut(s) 60, 66
BmrFI CCNGG 1 cut(s) 387
BmrI ACTGGG 1 cut(s) 619
BmsI GCATC 1 cut(s) 403
BmuI ACTGGG 1 cut(s) 619
BpiI GAAGAC 1 cut(s) 33
Bpu1102I GCTNAGC 1 cut(s) 498
BsaHI GRCGYC 2 cut(s) 59, 65
BsaJI CCNNGG 4 cut(s) 45, 228, 253, 403
Bsc4I CCNNNNNNNGG 4 cut(s) 74, 113, 473, 630
Bse118I RCCGGY 1 cut(s) 61
Bse1I ACTGG 4 cut(s) 310, 353, 467, 625
BseBI CCWGG 1 cut(s) 387
BseDI CCNNGG 4 cut(s) 45, 228, 253, 403
BseGI GGATG 1 cut(s) 160
BseLI CCNNNNNNNGG 4 cut(s) 74, 113, 473, 630
BseMII CTCAG 2 cut(s) 12, 158
BseNI ACTGG 4 cut(s) 310, 353, 467, 625
BsePI GCGCGC 1 cut(s) 557
BseRI GAGGAG 1 cut(s) 132
BseXI GCAGC 3 cut(s) 535, 573, 576
BsgI GTGCAG 2 cut(s) 384, 641
Bsh1236I CGCG 1 cut(s) 559
BshFI GGCC 2 cut(s) 224, 252
BshNI GGYRCC 2 cut(s) 58, 64
BsiSI CCGG 2 cut(s) 62, 598
BslFI GGGAC 1 cut(s) 89
BslI CCNNNNNNNGG 4 cut(s) 74, 113, 473, 630
BsmAI GTCTC 1 cut(s) 485
BsmFI GGGAC 1 cut(s) 89
BsnI GGCC 2 cut(s) 224, 252
Bsp1286I GDGCHC 1 cut(s) 322
Bsp143I GATC 2 cut(s) 121, 399
Bsp1720I GCTNAGC 1 cut(s) 498
Bsp19I CCATGG 1 cut(s) 45
BspACI CCGC 3 cut(s) 204, 207, 638
BspANI GGCC 2 cut(s) 224, 252
BspCNI CTCAG 2 cut(s) 13, 157
BspFNI CGCG 1 cut(s) 559
BspLI GGNNCC 2 cut(s) 60, 66
BspPI GGATC 1 cut(s) 394
BspT107I GGYRCC 2 cut(s) 58, 64
BsrBI CCGCTC 1 cut(s) 638
BsrFI RCCGGY 1 cut(s) 61
BsrI ACTGG 4 cut(s) 310, 353, 467, 625
BssAI RCCGGY 1 cut(s) 61
BssECI CCNNGG 4 cut(s) 45, 228, 253, 403
BssHII GCGCGC 1 cut(s) 557
BssMI GATC 2 cut(s) 121, 399
BssNI GRCGYC 2 cut(s) 59, 65
BssT1I CCWWGG 4 cut(s) 45, 228, 253, 403
Bst2UI CCWGG 1 cut(s) 387
Bst4CI ACNGT 2 cut(s) 132, 344
BstACI GRCGYC 2 cut(s) 59, 65
BstC8I GCNNGC 6 cut(s) 63, 115, 181, 418, 449, 559
BstDEI CTNAG 3 cut(s) 21, 144, 498
BstDSI CCRYGG 1 cut(s) 45
BstEII GGTNACC 1 cut(s) 8
BstENI CCTNNNNNAGG 1 cut(s) 111
BstF5I GGATG 1 cut(s) 160
BstFNI CGCG 1 cut(s) 559
BstH2I RGCGCY 2 cut(s) 62, 68
BstHHI GCGC 4 cut(s) 61, 67, 559, 561
BstKTI GATC 2 cut(s) 124, 402
BstMAI GTCTC 1 cut(s) 485
BstMBI GATC 2 cut(s) 121, 399
BstMWI GCNNNNNNNGC 2 cut(s) 204, 554
BstNI CCWGG 1 cut(s) 387
BstPI GGTNACC 1 cut(s) 8
BstSCI CCNGG 1 cut(s) 385
BstSFI CTRYAG 1 cut(s) 166
BstUI CGCG 1 cut(s) 559
BstV1I GCAGC 3 cut(s) 535, 573, 576
BstV2I GAAGAC 1 cut(s) 33
BsuRI GGCC 2 cut(s) 224, 252
BtgI CCRYGG 1 cut(s) 45
BtsCI GGATG 1 cut(s) 160
Cac8I GCNNGC 6 cut(s) 63, 115, 181, 418, 449, 559
CaiI CAGNNNCTG 1 cut(s) 473
CfoI GCGC 4 cut(s) 61, 67, 559, 561
Cfr10I RCCGGY 1 cut(s) 61
Cfr13I GGNCC 1 cut(s) 380
Csp6I GTAC 2 cut(s) 148, 340
CspCI CAANNNNNGTGG 2 cut(s) 214, 249
CviAII CATG 4 cut(s) 46, 328, 679, 693
CviQI GTAC 2 cut(s) 148, 340
DdeI CTNAG 3 cut(s) 21, 144, 498
DinI GGCGCC 2 cut(s) 60, 66
DpnI GATC 2 cut(s) 123, 401
DpnII GATC 2 cut(s) 121, 399
Eco130I CCWWGG 4 cut(s) 45, 228, 253, 403
Eco24I GRGCYC 1 cut(s) 322
Eco47I GGWCC 1 cut(s) 380
Eco91I GGTNACC 1 cut(s) 8
EcoNI CCTNNNNNAGG 1 cut(s) 111
EcoO65I GGTNACC 1 cut(s) 8
EcoRII CCWGG 1 cut(s) 385
EcoT14I CCWWGG 4 cut(s) 45, 228, 253, 403
EcoT38I GRGCYC 1 cut(s) 322
EgeI GGCGCC 2 cut(s) 60, 66
EheI GGCGCC 2 cut(s) 60, 66
ErhI CCWWGG 4 cut(s) 45, 228, 253, 403
FaeI CATG 4 cut(s) 49, 331, 682, 696
FalI AAGNNNNNCTT 2 cut(s) 507, 539
FaqI GGGAC 1 cut(s) 89
FatI CATG 4 cut(s) 45, 327, 678, 692
FblI GTMKAC 1 cut(s) 652
Fnu4HI GCNGC 4 cut(s) 205, 549, 562, 565
FokI GGATG 1 cut(s) 167
FriOI GRGCYC 1 cut(s) 322
Fsp4HI GCNGC 4 cut(s) 205, 549, 562, 565
FspBI CTAG 1 cut(s) 266
GlaI GCGC 4 cut(s) 60, 66, 558, 560
GluI GCNGC 4 cut(s) 205, 549, 562, 565
HaeII RGCGCY 2 cut(s) 62, 68
HaeIII GGCC 2 cut(s) 224, 252
HapII CCGG 2 cut(s) 62, 598
HhaI GCGC 4 cut(s) 61, 67, 559, 561
Hin1I GRCGYC 2 cut(s) 59, 65
Hin1II CATG 4 cut(s) 49, 331, 682, 696
Hin6I GCGC 4 cut(s) 59, 65, 557, 559
HinP1I GCGC 4 cut(s) 59, 65, 557, 559
HinfI GANTC 3 cut(s) 408, 482, 534
HpaII CCGG 2 cut(s) 62, 598
HphI GGTGA 3 cut(s) 20, 62, 488
Hpy166II GTNNAC 4 cut(s) 303, 340, 383, 653
Hpy188I TCNGA 1 cut(s) 399
Hpy188III TCNNGA 2 cut(s) 370, 437
Hpy8I GTNNAC 4 cut(s) 303, 340, 383, 653
HpyAV CCTTC 1 cut(s) 512
HpyCH4III ACNGT 2 cut(s) 132, 344
HpyCH4V TGCA 5 cut(s) 138, 236, 365, 551, 658
HpyF10VI GCNNNNNNNGC 2 cut(s) 204, 554
HpyF3I CTNAG 3 cut(s) 21, 144, 498
Hsp92I GRCGYC 2 cut(s) 59, 65
Hsp92II CATG 4 cut(s) 49, 331, 682, 696
HspAI GCGC 4 cut(s) 59, 65, 557, 559
KasI GGCGCC 2 cut(s) 58, 64
KroI GCCGGC 1 cut(s) 61
KroNI GCCGGC 1 cut(s) 63
Kzo9I GATC 2 cut(s) 121, 399
LmnI GCTCC 1 cut(s) 35
Lsp1109I GCAGC 3 cut(s) 535, 573, 576
LweI GCATC 1 cut(s) 403
MaeI CTAG 1 cut(s) 266
MaeIII GTNAC 1 cut(s) 8
MalI GATC 2 cut(s) 123, 401
MbiI CCGCTC 1 cut(s) 638
MboI GATC 2 cut(s) 121, 399
MboII GAAGA 1 cut(s) 38
MfeI CAATTG 2 cut(s) 84, 237
MhlI GDGCHC 1 cut(s) 322
MluCI AATT 4 cut(s) 84, 237, 279, 431
Mly113I GGCGCC 2 cut(s) 59, 65
MmeI TCCRAC 1 cut(s) 198
MnlI CCTC 7 cut(s) 16, 117, 153, 214, 438, 472, 675
MreI CGCCGGCG 1 cut(s) 61
MroNI GCCGGC 1 cut(s) 61
MroXI GAANNNNTTC 1 cut(s) 462
MseI TTAA 2 cut(s) 311, 512
MspI CCGG 2 cut(s) 62, 598
MspR9I CCNGG 1 cut(s) 387
MunI CAATTG 2 cut(s) 84, 237
MvaI CCWGG 1 cut(s) 387
MvnI CGCG 1 cut(s) 559
MwoI GCNNNNNNNGC 2 cut(s) 204, 554
NaeI GCCGGC 1 cut(s) 63
NarI GGCGCC 2 cut(s) 59, 65
NcoI CCATGG 1 cut(s) 45
NdeII GATC 2 cut(s) 121, 399
NgoMIV GCCGGC 1 cut(s) 61
NlaIII CATG 4 cut(s) 49, 331, 682, 696
NlaIV GGNNCC 2 cut(s) 60, 66
NmeAIII GCCGAG 1 cut(s) 93
NmuCI GTSAC 1 cut(s) 8
PauI GCGCGC 1 cut(s) 557
PdiI GCCGGC 1 cut(s) 63
PdmI GAANNNNTTC 1 cut(s) 462
PfeI GAWTC 3 cut(s) 408, 482, 534
PflMI CCANNNNNTGG 1 cut(s) 473
PkrI GCNGC 4 cut(s) 206, 550, 563, 566
PluTI GGCGCC 2 cut(s) 62, 68
Psp6I CCWGG 1 cut(s) 385
PspEI GGTNACC 1 cut(s) 8
PspGI CCWGG 1 cut(s) 385
PspN4I GGNNCC 2 cut(s) 60, 66
PspPI GGNCC 1 cut(s) 380
PstNI CAGNNNCTG 1 cut(s) 473
PteI GCGCGC 1 cut(s) 557
RsaI GTAC 2 cut(s) 149, 341
RsaNI GTAC 2 cut(s) 148, 340
SaqAI TTAA 2 cut(s) 311, 512
SatI GCNGC 4 cut(s) 205, 549, 562, 565
Sau3AI GATC 2 cut(s) 121, 399
Sau96I GGNCC 1 cut(s) 380
ScrFI CCNGG 1 cut(s) 387
SduI GDGCHC 1 cut(s) 322
SetI ASST 8 cut(s) 40, 100, 192, 234, 388, 504, 512, 523
SfaNI GCATC 1 cut(s) 403
SfcI CTRYAG 1 cut(s) 166
SfoI GGCGCC 2 cut(s) 60, 66
SgrAI CRCCGGYG 1 cut(s) 61
SinI GGWCC 1 cut(s) 380
Sse9I AATT 4 cut(s) 84, 237, 279, 431
SsiI CCGC 3 cut(s) 204, 207, 638
SspDI GGCGCC 2 cut(s) 58, 64
SspMI CTAG 1 cut(s) 266
StyD4I CCNGG 1 cut(s) 385
StyI CCWWGG 4 cut(s) 45, 228, 253, 403
TaaI ACNGT 2 cut(s) 132, 344
TasI AATT 4 cut(s) 84, 237, 279, 431
TatI WGTACW 1 cut(s) 147
TauI GCSGC 1 cut(s) 207
TfiI GAWTC 3 cut(s) 408, 482, 534
Tru1I TTAA 2 cut(s) 311, 512
Tru9I TTAA 2 cut(s) 311, 512
TseFI GTSAC 1 cut(s) 8
TseI GCWGC 3 cut(s) 548, 561, 564
Tsp45I GTSAC 1 cut(s) 8
TspDTI ATGAA 1 cut(s) 412
Van91I CCANNNNNTGG 1 cut(s) 473
VpaK11BI GGWCC 1 cut(s) 380
XagI CCTNNNNNAGG 1 cut(s) 111
XmiI GTMKAC 1 cut(s) 652
XmnI GAANNNNTTC 1 cut(s) 462
XspI CTAG 1 cut(s) 266
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.