Rorug07G0128400

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0002162
Physical Location & Seq
Reverse (-)
10118890 .. 10120898
2009 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0128400.1

Sequence Viewer

Length: 1476 bp
ATGGAGATTAACAAGTTAAGAGAAAACCTGGCCTCCCAAATCCCATTACTAGTGCCAGAGAACAACAGCTACATGATGAAACCCTACAACATGAAAGATGAGTTCATCCCATTTGAAGCTTCTTCTTCCTCAAAGGGTTACCTTCAAGATTTTCACCATCTTGATAATCAGTTTCATCAATATGCTAATGGGTCCTCATCAAATCCCATGTTTGGGGTCCAAAACCCATGTTTTGAACCTTATTACAATGACAACAATGCCTACACTTGTGACCAAGGCTCACCCACAGATGTAAATTTCTATGAGTGCAAGCCTCTAAACATTGCAGACAATTTGGGACATGGTCAAGTCTTGGACAATTTCCAAAGCTTGAATTTCCCACATCCCAGATCAAATTCCATTGGTCACATGATGGGGATTTCTCATAGGAGTGGTACTAATTTCCTGCCCTTGACAAATTCCCAGGACATTAAGCCTATGAGCTTTGTGGCACCAGATGAAGTCTCTTGCATCAACTCAGAAAATGGTTACTACAAAAGAGTTGGGATCAATCATAAGGGTACTAGTAGATTGTCTACAATAGCAAGGGCAGGGTCAACTTTTAAGGGTCGGAAGAAGAACAATGTGGTCAAGGGTCAATGGACAATTGACGAAGATAGGGTGTTGATTCAGCTGGTTGCACAGTATGGAGTGAGAAAGTGGTCACATATTGCTCAGATGTTGCCGGGAAGGATTGGCAAGCAGTGTAGAGAGAGATGGCATAACCACCTCAGGCCTGATATCAAGAAGGACACGTGGAGTGAGGAGGAAGACAAGATACTGATTCAAGCTCATGCAGAGATAGGAAACAAGTGGGCAGAGATTGCAAAGAGACTTCCCGGAAGGACTGAAAACTCCATCAAAAACCACTGGAATGCAACCAAAAGGAGACAATATTCGAAGCGAAAATGCCGCTCCAAGTACCCCAGAGGCTCTCTTCTTCAGGATTACATCAAGAGTTTGAACTTGGACGCGTCGCCAAACACCGGCCGCCAGAGAAAAAATAACAACAACAACAACAACAGCAGCAATAGTTCACCGGCCGATCACAACAATGCCAGTACTGCCAGCACCAAAGTTCAGTCACAAATCAATGTGGGGTTCAATAGTGAGAGTGATAGCAGGTTGGTTCCGAACTATGAGTTCAGTGAGGAGGACCCGGATTTTTGTTTCGATGAGAACATGTTTCAAGAGGGCTGCAGCATTGATTCTCTGCTTGATGACATTCCTTGTGCTACGGATTTCGGCAAGAATGGTAATGATTTTGATGGGAAAATGATGGGTTCATCAGATCATCAGCCTGTTGCTGTTGATGATGAGAAGAATTTCGAGGTGTTGGATATGCAAGTGGATTTGGGTTCTGCAGTGATGGAGAGTACTGAAGTGAAGAAGGAGCTGGACTTGGTGGAGATGATGTCTCAAGTGTCAAGTCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

491

Amino Acids

55.67

Weight (kDa)

6.03

Isoelectric Point (pI)

44.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-binding PF00249 211 - 257 1.8e-17 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 214 - 271 4.1e-19 Myb-like DNA-binding domain
Myb_DNA-binding PF00249 263 - 305 1.2e-17 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 266 - 317 2.2e-10 Myb-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1152
AccB1I GGYRCC 1 cut(s) 490
AccBSI CCGCTC 1 cut(s) 954
AccI GTMKAC 1 cut(s) 575
AccII CGCG 1 cut(s) 1013
AciI CCGC 2 cut(s) 952, 1030
AclWI GGATC 1 cut(s) 554
AcoI YGGCCR 2 cut(s) 1027, 1080
AcsI RAATTY 5 cut(s) 295, 373, 394, 457, 1363
AcuI CTGAAG 2 cut(s) 965, 1440
AcvI CACGTG 1 cut(s) 795
AfaI GTAC 5 cut(s) 436, 562, 962, 1102, 1417
AfiI CCNNNNNNNGG 3 cut(s) 212, 213, 1025
AflIII ACRYGT 3 cut(s) 792, 1011, 1221
AgsI TTSAA 8 cut(s) 116, 146, 236, 373, 827, 1003, 1144, 1229
AhlI ACTAGT 2 cut(s) 49, 563
AjnI CCWGG 2 cut(s) 27, 462
AluBI AGCT 7 cut(s) 69, 119, 369, 483, 673, 830, 1435
AluI AGCT 7 cut(s) 69, 119, 369, 483, 673, 830, 1435
Alw26I GTCTC 4 cut(s) 508, 865, 922, 1461
AlwI GGATC 1 cut(s) 554
AoxI GGCC 4 cut(s) 30, 773, 1027, 1080
ApeKI GCWGC 3 cut(s) 1065, 1236, 1239
ApoI RAATTY 5 cut(s) 295, 373, 394, 457, 1363
Asp700I GAANNNNTTC 1 cut(s) 1364
AspS9I GGNCC 3 cut(s) 192, 217, 1195
AsuC2I CCSGG 3 cut(s) 726, 879, 1199
AsuHPI GGTGA 3 cut(s) 146, 273, 1068
AsuII TTCGAA 1 cut(s) 938
AvaII GGWCC 3 cut(s) 192, 217, 1195
AxyI CCTNAGG 1 cut(s) 770
BanI GGYRCC 1 cut(s) 490
BarI GAAGNNNNNNTAC 2 cut(s) 801, 833
BbrPI CACGTG 1 cut(s) 795
BbsI GAAGAC 1 cut(s) 816
BbvI GCAGC 3 cut(s) 1077, 1223, 1251
BccI CCATC 7 cut(s) 165, 406, 750, 905, 1301, 1312, 1402
BciT130I CCWGG 2 cut(s) 29, 464
BcnI CCSGG 3 cut(s) 726, 879, 1199
BcoDI GTCTC 4 cut(s) 508, 865, 922, 1461
BcuI ACTAGT 2 cut(s) 49, 563
BfaI CTAG 2 cut(s) 50, 564
BfmI CTRYAG 2 cut(s) 1237, 1401
BfuAI ACCTGC 1 cut(s) 1152
BisI GCNGC 5 cut(s) 952, 1030, 1066, 1237, 1240
BlsI GCNGC 5 cut(s) 953, 1031, 1067, 1238, 1241
BmcAI AGTACT 2 cut(s) 1102, 1417
Bme1390I CCNGG 5 cut(s) 29, 464, 726, 879, 1199
Bme18I GGWCC 3 cut(s) 192, 217, 1195
BmgT120I GGNCC 3 cut(s) 192, 217, 1195
BmiI GGNNCC 5 cut(s) 193, 218, 492, 1170, 1197
BmrFI CCNGG 5 cut(s) 29, 464, 726, 879, 1199
BmsI GCATC 1 cut(s) 519
BpiI GAAGAC 1 cut(s) 816
Bpu14I TTCGAA 1 cut(s) 938
BpuEI CTTGAG 1 cut(s) 1443
BpuMI CCSGG 3 cut(s) 726, 879, 1199
BsaAI YACGTR 1 cut(s) 795
BsaJI CCNNGG 2 cut(s) 274, 462
BsaXI ACNNNNNCTCC 4 cut(s) 17, 47, 1439, 1469
Bsc4I CCNNNNNNNGG 3 cut(s) 212, 213, 1025
Bse118I RCCGGY 2 cut(s) 1025, 1078
Bse1I ACTGG 2 cut(s) 914, 1098
Bse21I CCTNAGG 1 cut(s) 770
Bse3DI GCAATG 1 cut(s) 321
BseBI CCWGG 2 cut(s) 29, 464
BseDI CCNNGG 2 cut(s) 274, 462
BseGI GGATG 2 cut(s) 105, 382
BseLI CCNNNNNNNGG 3 cut(s) 212, 213, 1025
BseMI GCAATG 1 cut(s) 321
BseMII CTCAG 3 cut(s) 531, 728, 784
BseNI ACTGG 2 cut(s) 914, 1098
BseRI GAGGAG 2 cut(s) 818, 1205
BseX3I CGGCCG 2 cut(s) 1027, 1080
BseXI GCAGC 3 cut(s) 1077, 1223, 1251
Bsh1236I CGCG 1 cut(s) 1013
Bsh1285I CGRYCG 2 cut(s) 1030, 1083
BshFI GGCC 4 cut(s) 32, 775, 1029, 1082
BshNI GGYRCC 1 cut(s) 490
BsiEI CGRYCG 2 cut(s) 1030, 1083
BsiSI CCGG 5 cut(s) 725, 879, 1026, 1079, 1199
BslFI GGGAC 1 cut(s) 351
BslI CCNNNNNNNGG 3 cut(s) 212, 213, 1025
BsmAI GTCTC 4 cut(s) 508, 865, 922, 1461
BsmFI GGGAC 1 cut(s) 351
BsmI GAATGC 1 cut(s) 919
BsnI GGCC 4 cut(s) 32, 775, 1029, 1082
Bsp119I TTCGAA 1 cut(s) 938
Bsp143I GATC 4 cut(s) 389, 546, 1084, 1330
BspACI CCGC 2 cut(s) 952, 1030
BspANI GGCC 4 cut(s) 32, 775, 1029, 1082
BspCNI CTCAG 3 cut(s) 530, 727, 783
BspFNI CGCG 1 cut(s) 1013
BspLI GGNNCC 5 cut(s) 193, 218, 492, 1170, 1197
BspMAI CTGCAG 2 cut(s) 1241, 1405
BspMI ACCTGC 1 cut(s) 1152
BspPI GGATC 1 cut(s) 554
BspT104I TTCGAA 1 cut(s) 938
BspT107I GGYRCC 1 cut(s) 490
BsrBI CCGCTC 1 cut(s) 954
BsrDI GCAATG 1 cut(s) 321
BsrFI RCCGGY 2 cut(s) 1025, 1078
BsrI ACTGG 2 cut(s) 914, 1098
BssAI RCCGGY 2 cut(s) 1025, 1078
BssECI CCNNGG 2 cut(s) 274, 462
BssMI GATC 4 cut(s) 389, 546, 1084, 1330
BssT1I CCWWGG 1 cut(s) 274
Bst2UI CCWGG 2 cut(s) 29, 464
Bst4CI ACNGT 1 cut(s) 684
Bst6I CTCTTC 1 cut(s) 981
BstAPI GCANNNNNTGC 1 cut(s) 863
BstBAI YACGTR 1 cut(s) 795
BstBI TTCGAA 1 cut(s) 938
BstC8I GCNNGC 3 cut(s) 311, 740, 1108
BstDEI CTNAG 3 cut(s) 517, 714, 770
BstEII GGTNACC 1 cut(s) 137
BstF5I GGATG 2 cut(s) 105, 382
BstFNI CGCG 1 cut(s) 1013
BstKTI GATC 4 cut(s) 392, 549, 1087, 1333
BstMAI GTCTC 4 cut(s) 508, 865, 922, 1461
BstMBI GATC 4 cut(s) 389, 546, 1084, 1330
BstMCI CGRYCG 2 cut(s) 1030, 1083
BstMWI GCNNNNNNNGC 2 cut(s) 863, 1103
BstNI CCWGG 2 cut(s) 29, 464
BstNSI RCATGY 1 cut(s) 1225
BstPI GGTNACC 1 cut(s) 137
BstSCI CCNGG 5 cut(s) 27, 462, 724, 877, 1197
BstSFI CTRYAG 2 cut(s) 1237, 1401
BstUI CGCG 1 cut(s) 1013
BstV1I GCAGC 3 cut(s) 1077, 1223, 1251
BstV2I GAAGAC 1 cut(s) 816
BstZI CGGCCG 2 cut(s) 1027, 1080
Bsu36I CCTNAGG 1 cut(s) 770
BsuRI GGCC 4 cut(s) 32, 775, 1029, 1082
BtsCI GGATG 2 cut(s) 105, 382
BtsI GCAGTG 2 cut(s) 749, 1410
BtsIMutI CAGTG 4 cut(s) 749, 907, 1192, 1410
BveI ACCTGC 1 cut(s) 1152
Cac8I GCNNGC 3 cut(s) 311, 740, 1108
Cfr10I RCCGGY 2 cut(s) 1025, 1078
Cfr13I GGNCC 3 cut(s) 192, 217, 1195
CseI GACGC 2 cut(s) 1002, 1019
Csp6I GTAC 5 cut(s) 435, 561, 961, 1101, 1416
CviAII CATG 8 cut(s) 73, 91, 208, 228, 341, 409, 833, 1222
CviQI GTAC 5 cut(s) 435, 561, 961, 1101, 1416
DdeI CTNAG 3 cut(s) 517, 714, 770
DpnI GATC 4 cut(s) 391, 548, 1086, 1332
DpnII GATC 4 cut(s) 389, 546, 1084, 1330
EaeI YGGCCR 2 cut(s) 1027, 1080
EagI CGGCCG 2 cut(s) 1027, 1080
Eam1104I CTCTTC 1 cut(s) 981
EarI CTCTTC 1 cut(s) 981
EclXI CGGCCG 2 cut(s) 1027, 1080
Eco130I CCWWGG 1 cut(s) 274
Eco147I AGGCCT 1 cut(s) 775
Eco32I GATATC 1 cut(s) 781
Eco47I GGWCC 3 cut(s) 192, 217, 1195
Eco52I CGGCCG 2 cut(s) 1027, 1080
Eco57I CTGAAG 2 cut(s) 965, 1440
Eco72I CACGTG 1 cut(s) 795
Eco81I CCTNAGG 1 cut(s) 770
Eco91I GGTNACC 1 cut(s) 137
EcoO109I RGGNCCY 2 cut(s) 192, 1195
EcoO65I GGTNACC 1 cut(s) 137
EcoRII CCWGG 2 cut(s) 27, 462
EcoRV GATATC 1 cut(s) 781
EcoT14I CCWWGG 1 cut(s) 274
ErhI CCWWGG 1 cut(s) 274
FaeI CATG 8 cut(s) 76, 94, 211, 231, 344, 412, 836, 1225
FaqI GGGAC 1 cut(s) 351
FatI CATG 8 cut(s) 72, 90, 207, 227, 340, 408, 832, 1221
FblI GTMKAC 1 cut(s) 575
Fnu4HI GCNGC 5 cut(s) 952, 1030, 1066, 1237, 1240
FokI GGATG 2 cut(s) 92, 369
Fsp4HI GCNGC 5 cut(s) 952, 1030, 1066, 1237, 1240
FspBI CTAG 2 cut(s) 50, 564
GluI GCNGC 5 cut(s) 952, 1030, 1066, 1237, 1240
HaeIII GGCC 4 cut(s) 32, 775, 1029, 1082
HapII CCGG 5 cut(s) 725, 879, 1026, 1079, 1199
HgaI GACGC 2 cut(s) 1002, 1019
Hin1II CATG 8 cut(s) 76, 94, 211, 231, 344, 412, 836, 1225
HincII GTYRAC 1 cut(s) 597
HindII GTYRAC 1 cut(s) 597
HindIII AAGCTT 2 cut(s) 117, 367
HinfI GANTC 3 cut(s) 667, 823, 1247
HpaII CCGG 5 cut(s) 725, 879, 1026, 1079, 1199
HphI GGTGA 3 cut(s) 146, 273, 1068
Hpy166II GTNNAC 3 cut(s) 576, 597, 1076
Hpy188I TCNGA 5 cut(s) 520, 612, 717, 1173, 1330
Hpy188III TCNNGA 6 cut(s) 146, 161, 784, 983, 994, 1229
Hpy8I GTNNAC 3 cut(s) 576, 597, 1076
Hpy99I CGWCG 1 cut(s) 1018
HpyAV CCTTC 5 cut(s) 152, 723, 781, 876, 1423
HpyCH4III ACNGT 1 cut(s) 684
HpyCH4IV ACGT 1 cut(s) 794
HpyF10VI GCNNNNNNNGC 2 cut(s) 863, 1103
HpyF3I CTNAG 3 cut(s) 517, 714, 770
HpySE526I ACGT 1 cut(s) 794
Hsp92II CATG 8 cut(s) 76, 94, 211, 231, 344, 412, 836, 1225
Kzo9I GATC 4 cut(s) 389, 546, 1084, 1330
LmnI GCTCC 2 cut(s) 959, 1432
Lsp1109I GCAGC 3 cut(s) 1077, 1223, 1251
LweI GCATC 1 cut(s) 519
MaeI CTAG 2 cut(s) 50, 564
MaeII ACGT 1 cut(s) 794
MaeIII GTNAC 6 cut(s) 137, 269, 404, 527, 702, 1122
MalI GATC 4 cut(s) 391, 548, 1086, 1332
MbiI CCGCTC 1 cut(s) 954
MboI GATC 4 cut(s) 389, 546, 1084, 1330
MfeI CAATTG 1 cut(s) 645
MluCI AATT 9 cut(s) 295, 331, 358, 373, 394, 439, 457, 645, 1363
MluI ACGCGT 1 cut(s) 1011
MmeI TCCRAC 2 cut(s) 590, 1356
MroXI GAANNNNTTC 1 cut(s) 1364
MseI TTAA 4 cut(s) 9, 17, 471, 603
MslI CAYNNNNRTG 4 cut(s) 180, 429, 912, 1092
MspA1I CMGCKG 1 cut(s) 673
MspI CCGG 5 cut(s) 725, 879, 1026, 1079, 1199
MspR9I CCNGG 5 cut(s) 29, 464, 726, 879, 1199
MunI CAATTG 1 cut(s) 645
Mva1269I GAATGC 1 cut(s) 919
MvaI CCWGG 2 cut(s) 29, 464
MvnI CGCG 1 cut(s) 1013
MwoI GCNNNNNNNGC 2 cut(s) 863, 1103
NciI CCSGG 3 cut(s) 726, 879, 1199
NdeII GATC 4 cut(s) 389, 546, 1084, 1330
NlaIII CATG 8 cut(s) 76, 94, 211, 231, 344, 412, 836, 1225
NlaIV GGNNCC 5 cut(s) 193, 218, 492, 1170, 1197
NmuCI GTSAC 4 cut(s) 269, 404, 702, 1122
NspI RCATGY 1 cut(s) 1225
NspV TTCGAA 1 cut(s) 938
PceI AGGCCT 1 cut(s) 775
PciI ACATGT 1 cut(s) 1221
PctI GAATGC 1 cut(s) 919
PdmI GAANNNNTTC 1 cut(s) 1364
PfeI GAWTC 3 cut(s) 667, 823, 1247
PflFI GACNNNGTC 1 cut(s) 342
PfoI TCCNGGA 1 cut(s) 877
PkrI GCNGC 5 cut(s) 953, 1031, 1067, 1238, 1241
PmaCI CACGTG 1 cut(s) 795
PmlI CACGTG 1 cut(s) 795
Ppu21I YACGTR 1 cut(s) 795
PpuMI RGGWCCY 2 cut(s) 192, 1195
PscI ACATGT 1 cut(s) 1221
Psp5II RGGWCCY 2 cut(s) 192, 1195
Psp6I CCWGG 2 cut(s) 27, 462
PspCI CACGTG 1 cut(s) 795
PspEI GGTNACC 1 cut(s) 137
PspGI CCWGG 2 cut(s) 27, 462
PspN4I GGNNCC 5 cut(s) 193, 218, 492, 1170, 1197
PspPI GGNCC 3 cut(s) 192, 217, 1195
PspPPI RGGWCCY 2 cut(s) 192, 1195
PsrI GAACNNNNNNTAC 2 cut(s) 53, 85
PstI CTGCAG 2 cut(s) 1241, 1405
PsyI GACNNNGTC 1 cut(s) 342
PvuII CAGCTG 1 cut(s) 673
RsaI GTAC 5 cut(s) 436, 562, 962, 1102, 1417
RsaNI GTAC 5 cut(s) 435, 561, 961, 1101, 1416
RseI CAYNNNNRTG 4 cut(s) 180, 429, 912, 1092
SaqAI TTAA 4 cut(s) 9, 17, 471, 603
SatI GCNGC 5 cut(s) 952, 1030, 1066, 1237, 1240
Sau3AI GATC 4 cut(s) 389, 546, 1084, 1330
Sau96I GGNCC 3 cut(s) 192, 217, 1195
ScaI AGTACT 2 cut(s) 1102, 1417
ScrFI CCNGG 5 cut(s) 29, 464, 726, 879, 1199
SfaNI GCATC 1 cut(s) 519
SfcI CTRYAG 2 cut(s) 1237, 1401
SfuI TTCGAA 1 cut(s) 938
SinI GGWCC 3 cut(s) 192, 217, 1195
SmiMI CAYNNNNRTG 4 cut(s) 180, 429, 912, 1092
SmlI CTYRAG 1 cut(s) 1458
SmoI CTYRAG 1 cut(s) 1458
SpeI ACTAGT 2 cut(s) 49, 563
Sse9I AATT 9 cut(s) 295, 331, 358, 373, 394, 439, 457, 645, 1363
SseBI AGGCCT 1 cut(s) 775
SsiI CCGC 2 cut(s) 952, 1030
SspI AATATT 1 cut(s) 935
SspMI CTAG 2 cut(s) 50, 564
StuI AGGCCT 1 cut(s) 775
StyD4I CCNGG 5 cut(s) 27, 462, 724, 877, 1197
StyI CCWWGG 1 cut(s) 274
TaaI ACNGT 1 cut(s) 684
TaiI ACGT 1 cut(s) 797
TaqI TCGA 3 cut(s) 938, 1212, 1368
TasI AATT 9 cut(s) 295, 331, 358, 373, 394, 439, 457, 645, 1363
TatI WGTACW 2 cut(s) 1100, 1415
TauI GCSGC 2 cut(s) 954, 1032
TfiI GAWTC 3 cut(s) 667, 823, 1247
Tru1I TTAA 4 cut(s) 9, 17, 471, 603
Tru9I TTAA 4 cut(s) 9, 17, 471, 603
TscAI CASTG 4 cut(s) 749, 914, 1192, 1410
TseFI GTSAC 4 cut(s) 269, 404, 702, 1122
TseI GCWGC 3 cut(s) 1065, 1236, 1239
Tsp45I GTSAC 4 cut(s) 269, 404, 702, 1122
TspDTI ATGAA 6 cut(s) 92, 94, 107, 164, 513, 1314
TspGWI ACGGA 1 cut(s) 1292
TspRI CASTG 4 cut(s) 749, 914, 1192, 1410
Tth111I GACNNNGTC 1 cut(s) 342
VpaK11BI GGWCC 3 cut(s) 192, 217, 1195
XapI RAATTY 5 cut(s) 295, 373, 394, 457, 1363
XceI RCATGY 1 cut(s) 1225
XmiI GTMKAC 1 cut(s) 575
XmnI GAANNNNTTC 1 cut(s) 1364
XspI CTAG 2 cut(s) 50, 564
ZrmI AGTACT 2 cut(s) 1102, 1417
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.