Rroxscaffold_2G00151140

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0002162
Physical Location & Seq
Forward (+)
88426668 .. 88427434
767 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00151140.1

Sequence Viewer

Length: 450 bp
ATGCCTATTCCCGACTCCTGCAAGGGAGATTTCTCGACTGCGATTGACTTCGTTGATGGTAGTCTTGTACCTTGGAAGGGAGGTAGTGCTCTCCTTCCTTCTCCCATTTTCCGTGGTGGTGACAACGACGGTATAGGTGGTATTGCGGTGACTCTACACCGGAGTTGCTTGTCGGCGACGATGATTATGAGTGTTGTGACATGTTCTAGCTCGAGTCGACTTGTTTTGGAGGCCGGAGAGGCGGCTCTAGTTACGGAAGCGAGGTTGGGTGTTGATCATGGCGTTCGAGGTGGCGGCGGCGGTGATCAAGATGGCGGCGGCGGCGGAGGTGCCCAAGTTGGCCGTAATTCTTCTAGGGTTTGTGTTGAATGGTTGTCTTTGGCCGGATCCGGGCTTGGGCGGGAGTCCTTGTTTGAGTTTCCTATTCCTGGGCTTGGTGGGCACTGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

149

Amino Acids

14.9

Weight (kDa)

4.99

Isoelectric Point (pI)

44.31

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 329
AccI GTMKAC 1 cut(s) 217
AclWI GGATC 2 cut(s) 381, 394
AcoI YGGCCR 2 cut(s) 340, 381
AfaI GTAC 1 cut(s) 69
AfiI CCNNNNNNNGG 5 cut(s) 77, 390, 396, 428, 434
AflIII ACRYGT 1 cut(s) 200
AgsI TTSAA 1 cut(s) 368
AjnI CCWGG 1 cut(s) 427
AluBI AGCT 1 cut(s) 210
AluI AGCT 1 cut(s) 210
Alw21I GWGCWC 1 cut(s) 91
AlwI GGATC 2 cut(s) 381, 394
Ama87I CYCGRG 1 cut(s) 211
AoxI GGCC 3 cut(s) 231, 340, 381
AsuC2I CCSGG 1 cut(s) 391
AsuHPI GGTGA 3 cut(s) 131, 160, 314
AvaI CYCGRG 1 cut(s) 211
BaeGI GKGCMC 2 cut(s) 334, 444
BamHI GGATCC 1 cut(s) 386
BanI GGYRCC 1 cut(s) 329
Bbv12I GWGCWC 1 cut(s) 91
BccI CCATC 2 cut(s) 50, 305
BceAI ACGGC 1 cut(s) 327
BciT130I CCWGG 1 cut(s) 429
BclI TGATCA 2 cut(s) 274, 304
BcnI CCSGG 1 cut(s) 391
BfaI CTAG 3 cut(s) 207, 248, 354
BglI GCCNNNNNGGC 1 cut(s) 239
BisI GCNGC 6 cut(s) 243, 295, 298, 316, 319, 322
BlsI GCNGC 6 cut(s) 244, 296, 299, 317, 320, 323
Bme1390I CCNGG 2 cut(s) 391, 429
BmeT110I CYCGRG 1 cut(s) 211
BmiI GGNNCC 2 cut(s) 331, 388
BmrFI CCNGG 2 cut(s) 391, 429
BpuMI CCSGG 1 cut(s) 391
BsaJI CCNNGG 3 cut(s) 71, 112, 428
BsaWI WCCGGW 1 cut(s) 159
BsaXI ACNNNNNCTCC 2 cut(s) 395, 425
Bsc4I CCNNNNNNNGG 5 cut(s) 77, 390, 396, 428, 434
BseBI CCWGG 1 cut(s) 429
BseDI CCNNGG 3 cut(s) 71, 112, 428
BseLI CCNNNNNNNGG 5 cut(s) 77, 390, 396, 428, 434
BseSI GKGCMC 2 cut(s) 334, 444
BshFI GGCC 3 cut(s) 233, 342, 383
BshNI GGYRCC 1 cut(s) 329
BsiHKAI GWGCWC 1 cut(s) 91
BsiHKCI CYCGRG 1 cut(s) 211
BsiSI CCGG 4 cut(s) 160, 234, 384, 390
BslI CCNNNNNNNGG 5 cut(s) 77, 390, 396, 428, 434
BsnI GGCC 3 cut(s) 233, 342, 383
BsoBI CYCGRG 1 cut(s) 211
Bsp1286I GDGCHC 3 cut(s) 91, 334, 444
Bsp143I GATC 3 cut(s) 274, 304, 386
BspANI GGCC 3 cut(s) 233, 342, 383
BspLI GGNNCC 2 cut(s) 331, 388
BspPI GGATC 2 cut(s) 381, 394
BspT107I GGYRCC 1 cut(s) 329
BssECI CCNNGG 3 cut(s) 71, 112, 428
BssMI GATC 3 cut(s) 274, 304, 386
BssT1I CCWWGG 1 cut(s) 71
Bst2UI CCWGG 1 cut(s) 429
Bst4CI ACNGT 2 cut(s) 131, 446
BstDSI CCRYGG 1 cut(s) 112
BstKTI GATC 3 cut(s) 277, 307, 389
BstMBI GATC 3 cut(s) 274, 304, 386
BstMWI GCNNNNNNNGC 3 cut(s) 239, 321, 439
BstNI CCWGG 1 cut(s) 429
BstNSI RCATGY 1 cut(s) 204
BstSCI CCNGG 2 cut(s) 389, 427
BstSLI GKGCMC 2 cut(s) 334, 444
BstX2I RGATCY 1 cut(s) 386
BstYI RGATCY 1 cut(s) 386
BsuRI GGCC 3 cut(s) 233, 342, 383
BtgI CCRYGG 1 cut(s) 112
BtsIMutI CAGTG 1 cut(s) 442
Csp6I GTAC 1 cut(s) 68
CviAII CATG 2 cut(s) 201, 278
CviJI RGCY 7 cut(s) 210, 233, 245, 342, 383, 394, 433
CviKI_1 RGCY 7 cut(s) 210, 233, 245, 342, 383, 394, 433
CviQI GTAC 1 cut(s) 68
DpnI GATC 3 cut(s) 276, 306, 388
DpnII GATC 3 cut(s) 274, 304, 386
EaeI YGGCCR 2 cut(s) 340, 381
EciI GGCGGA 1 cut(s) 339
Eco130I CCWWGG 1 cut(s) 71
Eco88I CYCGRG 1 cut(s) 211
EcoRII CCWGG 1 cut(s) 427
EcoT14I CCWWGG 1 cut(s) 71
ErhI CCWWGG 1 cut(s) 71
FaeI CATG 2 cut(s) 204, 281
FaiI YATR 4 cut(s) 134, 188, 202, 279
FatI CATG 2 cut(s) 200, 277
FauI CCCGC 1 cut(s) 393
FbaI TGATCA 2 cut(s) 274, 304
FblI GTMKAC 1 cut(s) 217
Fnu4HI GCNGC 6 cut(s) 243, 295, 298, 316, 319, 322
Fsp4HI GCNGC 6 cut(s) 243, 295, 298, 316, 319, 322
FspBI CTAG 3 cut(s) 207, 248, 354
GluI GCNGC 6 cut(s) 243, 295, 298, 316, 319, 322
HaeIII GGCC 3 cut(s) 233, 342, 383
HapII CCGG 4 cut(s) 160, 234, 384, 390
Hin1II CATG 2 cut(s) 204, 281
HincII GTYRAC 1 cut(s) 218
HindII GTYRAC 1 cut(s) 218
HinfI GANTC 4 cut(s) 14, 151, 214, 404
HpaII CCGG 4 cut(s) 160, 234, 384, 390
HphI GGTGA 3 cut(s) 131, 160, 314
Hpy166II GTNNAC 1 cut(s) 218
Hpy188III TCNNGA 3 cut(s) 11, 34, 308
Hpy8I GTNNAC 1 cut(s) 218
Hpy99I CGWCG 2 cut(s) 131, 181
HpyAV CCTTC 3 cut(s) 70, 104, 108
HpyCH4III ACNGT 2 cut(s) 131, 446
HpyCH4V TGCA 1 cut(s) 21
HpyF10VI GCNNNNNNNGC 3 cut(s) 239, 321, 439
Hsp92II CATG 2 cut(s) 204, 281
Ksp22I TGATCA 2 cut(s) 274, 304
Kzo9I GATC 3 cut(s) 274, 304, 386
LpnPI CCDG 7 cut(s) 31, 173, 247, 397, 403, 414, 441
MaeI CTAG 3 cut(s) 207, 248, 354
MaeIII GTNAC 4 cut(s) 119, 148, 196, 250
MalI GATC 3 cut(s) 276, 306, 388
MboI GATC 3 cut(s) 274, 304, 386
MboII GAAGA 1 cut(s) 342
MflI RGATCY 1 cut(s) 386
MhlI GDGCHC 3 cut(s) 91, 334, 444
MluCI AATT 1 cut(s) 346
MlyI GAGTC 4 cut(s) 8, 145, 223, 413
MnlI CCTC 6 cut(s) 74, 223, 232, 255, 281, 320
MspI CCGG 4 cut(s) 160, 234, 384, 390
MspR9I CCNGG 2 cut(s) 391, 429
MvaI CCWGG 1 cut(s) 429
MwoI GCNNNNNNNGC 3 cut(s) 239, 321, 439
NciI CCSGG 1 cut(s) 391
NdeII GATC 3 cut(s) 274, 304, 386
NlaIII CATG 2 cut(s) 204, 281
NlaIV GGNNCC 2 cut(s) 331, 388
NmuCI GTSAC 3 cut(s) 119, 148, 196
NspI RCATGY 1 cut(s) 204
PaeR7I CTCGAG 1 cut(s) 211
PciI ACATGT 1 cut(s) 200
PkrI GCNGC 6 cut(s) 244, 296, 299, 317, 320, 323
PleI GAGTC 4 cut(s) 8, 145, 222, 412
PpsI GAGTC 4 cut(s) 8, 145, 222, 412
PscI ACATGT 1 cut(s) 200
Psp6I CCWGG 1 cut(s) 427
PspGI CCWGG 1 cut(s) 427
PspN4I GGNNCC 2 cut(s) 331, 388
PspXI VCTCGAGB 1 cut(s) 211
PsuI RGATCY 1 cut(s) 386
RsaI GTAC 1 cut(s) 69
RsaNI GTAC 1 cut(s) 68
SalI GTCGAC 1 cut(s) 216
SatI GCNGC 6 cut(s) 243, 295, 298, 316, 319, 322
Sau3AI GATC 3 cut(s) 274, 304, 386
SchI GAGTC 4 cut(s) 8, 145, 223, 413
ScrFI CCNGG 2 cut(s) 391, 429
SduI GDGCHC 3 cut(s) 91, 334, 444
SetI ASST 7 cut(s) 73, 85, 139, 212, 266, 292, 331
Sfr274I CTCGAG 1 cut(s) 211
SlaI CTCGAG 1 cut(s) 211
SmlI CTYRAG 1 cut(s) 211
SmoI CTYRAG 1 cut(s) 211
Sse9I AATT 1 cut(s) 346
SspMI CTAG 3 cut(s) 207, 248, 354
StyD4I CCNGG 2 cut(s) 389, 427
StyI CCWWGG 1 cut(s) 71
TaaI ACNGT 2 cut(s) 131, 446
TaqI TCGA 4 cut(s) 35, 212, 217, 286
TasI AATT 1 cut(s) 346
TauI GCSGC 6 cut(s) 245, 297, 300, 318, 321, 324
TscAI CASTG 1 cut(s) 449
TseFI GTSAC 3 cut(s) 119, 148, 196
Tsp45I GTSAC 3 cut(s) 119, 148, 196
TspGWI ACGGA 2 cut(s) 101, 269
TspRI CASTG 1 cut(s) 449
XceI RCATGY 1 cut(s) 204
XhoI CTCGAG 1 cut(s) 211
XmiI GTMKAC 1 cut(s) 217
XspI CTAG 3 cut(s) 207, 248, 354
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.