Rmu_sc0005690.1_g000014

short-chain dehydrogenase reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005690.1
OG0002162
Physical Location & Seq
Forward (+)
49974 .. 52135
2162 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 738 bp
atgatcatgagtgctataacatgttctggcctgagtcgacttgttttggaggctggagaggtgcctctagttccggaagcaagttgggtgttgatcatggtgttcgaggtggcggcagcggtgatcaagatggcggcgacggcagaggcacccaagttggctgtaatttttctagggtttgtgttgaatggttgtcttgggcctggatctgggcttggttgggagtccttgtttgggtttcctattcctaggcttggaccaagagggtcaggacgtcgcttcttcgatcggccctcactgcacctacatccactgcgacgtaacagacgaggatcaagtgaagagcctgatcaaatcaacggtccagatgcacggccgcctggacatcatgttcagcaacgctggaatattctgctgtccgagcagacggtgatggagtttgacttatccaagtacgacaagctcatggcggtcaatttacgtgggatggtggcgtgtgtgaagcacgcggcgcgggccatggtggaaggtggagttaaggggagcgtgacatgcacggcgagcgttgtagcgactactggattagagtggttccgactacaccatgtcaaagcacgccattttggggctggtgaggtcggcgagtgtccagctcggtgcgtgaactgcgtgtcgccggggccttgtcatacgccgatcatggactcgttgttgccttcaaggatgtggaggagataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
adh_short_C2

Protein Analysis

245

Amino Acids

26.66

Weight (kDa)

9.2

Isoelectric Point (pI)

51.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short_C2 PF13561 142 - 237 4.4e-08 Enoyl-(Acyl carrier protein) reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 277
AccB1I GGYRCC 2 cut(s) 61, 148
AccI GTMKAC 1 cut(s) 37
AccII CGCG 2 cut(s) 509, 514
AccIII TCCGGA 1 cut(s) 73
AciI CCGC 7 cut(s) 113, 119, 134, 377, 470, 509, 514
AclWI GGATC 2 cut(s) 214, 340
AcoI YGGCCR 1 cut(s) 374
AcyI GRCGYC 1 cut(s) 274
AfaI GTAC 1 cut(s) 455
AfiI CCNNNNNNNGG 4 cut(s) 209, 234, 254, 625
AflIII ACRYGT 1 cut(s) 20
AgsI TTSAA 2 cut(s) 187, 720
AjnI CCWGG 2 cut(s) 202, 379
AloI GAACNNNNNNTCC 2 cut(s) 375, 407
AluBI AGCT 2 cut(s) 463, 653
AluI AGCT 2 cut(s) 463, 653
AlwI GGATC 2 cut(s) 214, 340
Aor13HI TCCGGA 1 cut(s) 73
AoxI GGCC 6 cut(s) 28, 200, 290, 374, 516, 680
ApeKI GCWGC 1 cut(s) 116
ArsI GACNNNNNNTTYG 2 cut(s) 346, 378
AspA2I CCTAGG 1 cut(s) 248
AspLEI GCGC 1 cut(s) 514
AspS9I GGNCC 6 cut(s) 200, 257, 291, 362, 516, 680
AsuC2I CCSGG 1 cut(s) 678
AsuHPI GGTGA 3 cut(s) 133, 442, 644
AvaII GGWCC 2 cut(s) 257, 362
AvrII CCTAGG 1 cut(s) 248
BanI GGYRCC 2 cut(s) 61, 148
BbvI GCAGC 1 cut(s) 128
BccI CCATC 3 cut(s) 124, 427, 481
BceAI ACGGC 3 cut(s) 156, 389, 573
BciT130I CCWGG 2 cut(s) 204, 381
BclI TGATCA 4 cut(s) 3, 93, 123, 349
BcnI CCSGG 1 cut(s) 678
BfaI CTAG 3 cut(s) 68, 173, 249
BisI GCNGC 5 cut(s) 114, 117, 135, 377, 510
BlnI CCTAGG 1 cut(s) 248
BlsI GCNGC 5 cut(s) 115, 118, 136, 378, 511
Bme1390I CCNGG 3 cut(s) 204, 381, 678
Bme18I GGWCC 2 cut(s) 257, 362
BmgT120I GGNCC 6 cut(s) 200, 257, 291, 362, 516, 680
BmiI GGNNCC 4 cut(s) 63, 150, 593, 681
BmrFI CCNGG 3 cut(s) 204, 381, 678
BmsI GCATC 1 cut(s) 358
BpmI CTGGAG 1 cut(s) 75
BpuMI CCSGG 1 cut(s) 678
BsaAI YACGTR 1 cut(s) 482
BsaHI GRCGYC 1 cut(s) 274
BsaJI CCNNGG 3 cut(s) 248, 519, 677
BsaWI WCCGGW 1 cut(s) 73
BsaXI ACNNNNNCTCC 2 cut(s) 215, 245
Bsc4I CCNNNNNNNGG 4 cut(s) 209, 234, 254, 625
Bse1I ACTGG 1 cut(s) 583
BseAI TCCGGA 1 cut(s) 73
BseBI CCWGG 2 cut(s) 204, 381
BseDI CCNNGG 3 cut(s) 248, 519, 677
BseGI GGATG 3 cut(s) 307, 492, 729
BseLI CCNNNNNNNGG 4 cut(s) 209, 234, 254, 625
BseMII CTCAG 1 cut(s) 23
BseNI ACTGG 1 cut(s) 583
BseX3I CGGCCG 1 cut(s) 374
BseXI GCAGC 1 cut(s) 128
BsgI GTGCAG 1 cut(s) 284
Bsh1236I CGCG 2 cut(s) 509, 514
Bsh1285I CGRYCG 2 cut(s) 289, 377
BshFI GGCC 6 cut(s) 30, 202, 292, 376, 518, 682
BshNI GGYRCC 2 cut(s) 61, 148
BsiEI CGRYCG 2 cut(s) 289, 377
BsiSI CCGG 2 cut(s) 74, 677
BslI CCNNNNNNNGG 4 cut(s) 209, 234, 254, 625
BsnI GGCC 6 cut(s) 30, 202, 292, 376, 518, 682
Bsp13I TCCGGA 1 cut(s) 73
Bsp143I GATC 8 cut(s) 3, 93, 123, 206, 286, 332, 349, 696
Bsp19I CCATGG 1 cut(s) 519
BspACI CCGC 7 cut(s) 113, 119, 134, 377, 470, 509, 514
BspANI GGCC 6 cut(s) 30, 202, 292, 376, 518, 682
BspCNI CTCAG 1 cut(s) 24
BspEI TCCGGA 1 cut(s) 73
BspFNI CGCG 2 cut(s) 509, 514
BspHI TCATGA 1 cut(s) 6
BspLI GGNNCC 4 cut(s) 63, 150, 593, 681
BspPI GGATC 2 cut(s) 214, 340
BspQI GCTCTTC 1 cut(s) 336
BspT107I GGYRCC 2 cut(s) 61, 148
BsrI ACTGG 1 cut(s) 583
BssECI CCNNGG 3 cut(s) 248, 519, 677
BssMI GATC 8 cut(s) 3, 93, 123, 206, 286, 332, 349, 696
BssNI GRCGYC 1 cut(s) 274
BssT1I CCWWGG 2 cut(s) 248, 519
Bst2UI CCWGG 2 cut(s) 204, 381
Bst4CI ACNGT 2 cut(s) 362, 430
Bst6I CTCTTC 1 cut(s) 336
BstACI GRCGYC 1 cut(s) 274
BstBAI YACGTR 1 cut(s) 482
BstC8I GCNNGC 4 cut(s) 507, 516, 562, 616
BstDEI CTNAG 1 cut(s) 32
BstDSI CCRYGG 1 cut(s) 519
BstF5I GGATG 3 cut(s) 307, 492, 729
BstFNI CGCG 2 cut(s) 509, 514
BstHHI GCGC 1 cut(s) 514
BstKTI GATC 8 cut(s) 6, 96, 126, 209, 289, 335, 352, 699
BstMBI GATC 8 cut(s) 3, 93, 123, 206, 286, 332, 349, 696
BstMCI CGRYCG 2 cut(s) 289, 377
BstMWI GCNNNNNNNGC 8 cut(s) 140, 298, 421, 511, 515, 552, 561, 666
BstNI CCWGG 2 cut(s) 204, 381
BstNSI RCATGY 2 cut(s) 24, 555
BstSCI CCNGG 3 cut(s) 202, 379, 676
BstUI CGCG 2 cut(s) 509, 514
BstV1I GCAGC 1 cut(s) 128
BstX2I RGATCY 1 cut(s) 206
BstYI RGATCY 1 cut(s) 206
BstZI CGGCCG 1 cut(s) 374
BsuRI GGCC 6 cut(s) 30, 202, 292, 376, 518, 682
BtgI CCRYGG 1 cut(s) 519
BtsCI GGATG 3 cut(s) 307, 492, 729
BtsI GCAGTG 2 cut(s) 296, 311
BtsIMutI CAGTG 2 cut(s) 296, 311
Cac8I GCNNGC 4 cut(s) 507, 516, 562, 616
CciI TCATGA 1 cut(s) 6
CfoI GCGC 1 cut(s) 514
Cfr13I GGNCC 6 cut(s) 200, 257, 291, 362, 516, 680
Csp6I GTAC 1 cut(s) 454
CspCI CAANNNNNGTGG 2 cut(s) 463, 498
CviAII CATG 9 cut(s) 7, 21, 97, 389, 466, 520, 552, 605, 700
CviQI GTAC 1 cut(s) 454
DdeI CTNAG 1 cut(s) 32
DpnI GATC 8 cut(s) 5, 95, 125, 208, 288, 334, 351, 698
DpnII GATC 8 cut(s) 3, 93, 123, 206, 286, 332, 349, 696
EaeI YGGCCR 1 cut(s) 374
EagI CGGCCG 1 cut(s) 374
Eam1104I CTCTTC 1 cut(s) 336
EarI CTCTTC 1 cut(s) 336
EclXI CGGCCG 1 cut(s) 374
Eco130I CCWWGG 2 cut(s) 248, 519
Eco47I GGWCC 2 cut(s) 257, 362
Eco52I CGGCCG 1 cut(s) 374
EcoO109I RGGNCCY 1 cut(s) 680
EcoRII CCWGG 2 cut(s) 202, 379
EcoT14I CCWWGG 2 cut(s) 248, 519
ErhI CCWWGG 2 cut(s) 248, 519
FaeI CATG 9 cut(s) 10, 24, 100, 392, 469, 523, 555, 608, 703
FatI CATG 9 cut(s) 6, 20, 96, 388, 465, 519, 551, 604, 699
FauI CCCGC 1 cut(s) 507
FbaI TGATCA 4 cut(s) 3, 93, 123, 349
FblI GTMKAC 1 cut(s) 37
Fnu4HI GCNGC 5 cut(s) 114, 117, 135, 377, 510
FokI GGATG 2 cut(s) 294, 499
Fsp4HI GCNGC 5 cut(s) 114, 117, 135, 377, 510
FspBI CTAG 3 cut(s) 68, 173, 249
GlaI GCGC 1 cut(s) 513
GluI GCNGC 5 cut(s) 114, 117, 135, 377, 510
GsuI CTGGAG 1 cut(s) 75
HaeIII GGCC 6 cut(s) 30, 202, 292, 376, 518, 682
HapII CCGG 2 cut(s) 74, 677
HhaI GCGC 1 cut(s) 514
Hin1I GRCGYC 1 cut(s) 274
Hin1II CATG 9 cut(s) 10, 24, 100, 392, 469, 523, 555, 608, 703
Hin6I GCGC 1 cut(s) 512
HinP1I GCGC 1 cut(s) 512
HincII GTYRAC 1 cut(s) 38
HindII GTYRAC 1 cut(s) 38
HinfI GANTC 3 cut(s) 34, 224, 704
HpaII CCGG 2 cut(s) 74, 677
HphI GGTGA 3 cut(s) 133, 442, 644
Hpy166II GTNNAC 2 cut(s) 38, 664
Hpy188I TCNGA 2 cut(s) 421, 596
Hpy188III TCNNGA 5 cut(s) 7, 74, 127, 270, 365
Hpy8I GTNNAC 2 cut(s) 38, 664
Hpy99I CGWCG 3 cut(s) 142, 279, 321
HpyAV CCTTC 2 cut(s) 521, 726
HpyCH4III ACNGT 2 cut(s) 362, 430
HpyCH4IV ACGT 3 cut(s) 274, 319, 481
HpyCH4V TGCA 3 cut(s) 301, 371, 555
HpyF10VI GCNNNNNNNGC 8 cut(s) 140, 298, 421, 511, 515, 552, 561, 666
HpyF3I CTNAG 1 cut(s) 32
HpySE526I ACGT 3 cut(s) 274, 319, 481
Hsp92I GRCGYC 1 cut(s) 274
Hsp92II CATG 9 cut(s) 10, 24, 100, 392, 469, 523, 555, 608, 703
HspAI GCGC 1 cut(s) 512
Kpn2I TCCGGA 1 cut(s) 73
Ksp22I TGATCA 4 cut(s) 3, 93, 123, 349
Kzo9I GATC 8 cut(s) 3, 93, 123, 206, 286, 332, 349, 696
LguI GCTCTTC 1 cut(s) 336
LmnI GCTCC 1 cut(s) 543
Lsp1109I GCAGC 1 cut(s) 128
LweI GCATC 1 cut(s) 358
MaeI CTAG 3 cut(s) 68, 173, 249
MaeII ACGT 3 cut(s) 274, 319, 481
MaeIII GTNAC 2 cut(s) 320, 547
MalI GATC 8 cut(s) 5, 95, 125, 208, 288, 334, 351, 698
MboI GATC 8 cut(s) 3, 93, 123, 206, 286, 332, 349, 696
MboII GAAGA 2 cut(s) 274, 353
MflI RGATCY 1 cut(s) 206
MluCI AATT 2 cut(s) 165, 475
MlyI GAGTC 3 cut(s) 43, 233, 698
MmeI TCCRAC 1 cut(s) 619
MroI TCCGGA 1 cut(s) 73
MseI TTAA 1 cut(s) 537
MspA1I CMGCKG 1 cut(s) 119
MspI CCGG 2 cut(s) 74, 677
MspR9I CCNGG 3 cut(s) 204, 381, 678
MvaI CCWGG 2 cut(s) 204, 381
MvnI CGCG 2 cut(s) 509, 514
MwoI GCNNNNNNNGC 8 cut(s) 140, 298, 421, 511, 515, 552, 561, 666
NciI CCSGG 1 cut(s) 678
NcoI CCATGG 1 cut(s) 519
NdeII GATC 8 cut(s) 3, 93, 123, 206, 286, 332, 349, 696
NlaIII CATG 9 cut(s) 10, 24, 100, 392, 469, 523, 555, 608, 703
NlaIV GGNNCC 4 cut(s) 63, 150, 593, 681
NmuCI GTSAC 1 cut(s) 547
NspI RCATGY 2 cut(s) 24, 555
PagI TCATGA 1 cut(s) 6
PciI ACATGT 1 cut(s) 20
PciSI GCTCTTC 1 cut(s) 336
PcsI WCGNNNNNNNCGW 1 cut(s) 325
PkrI GCNGC 5 cut(s) 115, 118, 136, 378, 511
Ple19I CGATCG 1 cut(s) 289
PleI GAGTC 3 cut(s) 42, 232, 698
PpsI GAGTC 3 cut(s) 42, 232, 698
Ppu21I YACGTR 1 cut(s) 482
PscI ACATGT 1 cut(s) 20
Psp6I CCWGG 2 cut(s) 202, 379
PspGI CCWGG 2 cut(s) 202, 379
PspN4I GGNNCC 4 cut(s) 63, 150, 593, 681
PspPI GGNCC 6 cut(s) 200, 257, 291, 362, 516, 680
PsuI RGATCY 1 cut(s) 206
PvuI CGATCG 1 cut(s) 289
RsaI GTAC 1 cut(s) 455
RsaNI GTAC 1 cut(s) 454
SalI GTCGAC 1 cut(s) 36
SapI GCTCTTC 1 cut(s) 336
SaqAI TTAA 1 cut(s) 537
SatI GCNGC 5 cut(s) 114, 117, 135, 377, 510
Sau3AI GATC 8 cut(s) 3, 93, 123, 206, 286, 332, 349, 696
Sau96I GGNCC 6 cut(s) 200, 257, 291, 362, 516, 680
SchI GAGTC 3 cut(s) 43, 233, 698
ScrFI CCNGG 3 cut(s) 204, 381, 678
SfaNI GCATC 1 cut(s) 358
SinI GGWCC 2 cut(s) 257, 362
Sse9I AATT 2 cut(s) 165, 475
SsiI CCGC 7 cut(s) 113, 119, 134, 377, 470, 509, 514
SspI AATATT 1 cut(s) 409
SspMI CTAG 3 cut(s) 68, 173, 249
StyD4I CCNGG 3 cut(s) 202, 379, 676
StyI CCWWGG 2 cut(s) 248, 519
TaaI ACNGT 2 cut(s) 362, 430
TaiI ACGT 3 cut(s) 277, 322, 484
TaqI TCGA 3 cut(s) 37, 105, 285
TasI AATT 2 cut(s) 165, 475
TauI GCSGC 4 cut(s) 116, 137, 379, 512
Tru1I TTAA 1 cut(s) 537
Tru9I TTAA 1 cut(s) 537
TscAI CASTG 2 cut(s) 303, 318
TseFI GTSAC 1 cut(s) 547
TseI GCWGC 1 cut(s) 116
Tsp45I GTSAC 1 cut(s) 547
TspRI CASTG 2 cut(s) 303, 318
VpaK11BI GGWCC 2 cut(s) 257, 362
XceI RCATGY 2 cut(s) 24, 555
XcmI CCANNNNNNNNNTGG 1 cut(s) 626
XmaJI CCTAGG 1 cut(s) 248
XmiI GTMKAC 1 cut(s) 37
XspI CTAG 3 cut(s) 68, 173, 249
ZraI GACGTC 1 cut(s) 275
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.