Rmu_sc0006422.1_g000029

RRP12-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0006422.1
OG0000835
Physical Location & Seq
Forward (+)
118727 .. 121059
2333 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0006422.1_g000029.1.cds

Sequence Viewer

Length: 1632 bp
atggaaaccctaaaccccaccaccagcaccaccgtggacgacggcgcggctaacatcggcttctcaatcttgatgagcttctctgacagccgtcgcgaaatcgacctcgaaatccgcaagctcataaccgtcatgtgccaggtattccgagaccacaaggctcctccgacgccgctggcctacttgggcgcaacgtgctcctctctcgacctgatcgccgcttcgaagccccgaccttccgatgacgtgctccacgcccacatcataattctgtccatcgtcatccccaaagtcccgccagcatttctcatcaacaatctccagttggtctgcaattccttagccccagctcttcgttcttcatccccaagcttcgacggtgccgcctgcacaggagttaaatgcatagctcaattgctcattagcagcactaactgcgtcaacaactggtcacaagtttcgaaacccttcaggctcttgttcagattcgcttatgtctccagcatcgaggtaagagaacaagcaggtttgtgtgttcacgacacactgcaaagttttcgaggaactcccctgtttggccctgcaaataaaaccattactgacttgttaatgaaaaagtatcctccgccacgtgtggaatcaaatactgatggagttaatagcatcgaacaacgcaaggccatgcatactttgaatcttgtgatgctatcccttggcgctatgtcaaccgaggacagaactgctgtgctcgtctactttatggatctcttagaactgcaccatacctatgttacaagactcatcacagatgctctgtacagattctgcctcaatccatcatcaccagatgttgacctcgaattgttgctcgatctaatatgctcaatatgtctttctgtctcccaacacaagacgaattacatcatgaaaactatagctggtttgctcaattttggagtgcctaaagtctattccctgaacaagcaaatctgcaaaattaaactccctattatgtttgatgcactcattggtatagactcttgttttccctcaactctactgtgtgctacttttccaaagttactttcttatcgctgccctactatccggtaccttgtttttgaaatagtctcaacaatgttcaataaattaggagtatactcttcttatttgatgaggggtacagtcaagaccttggcagacatgcagattttgcctgatagagaattccctttcaaggaagaggttaagattcttctagaaatggttagtgagaaatgtgggcttgatgctatttcggccactatccctcaagaacacatggaactgcttgcagagatcaatcaacaactgggttctaaatctgtggatgcgagttctcatgtatccaaagcaactatatctgggaataacttatctcagtccaagaatatggacaattatcgtcgatactacaatatgaaacggaaattggctggtaaagagagtttgtctgatcgccagtggagagcatatgactcatttgacactttggtcactgaagtttcaaatatatcactttggtcactcaactattacattgtcaatcacctaagtcactttgttaattttttttcattaaaaaaattataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NUC173

Protein Analysis

543

Amino Acids

60.77

Weight (kDa)

8.39

Isoelectric Point (pI)

41.35

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1630
AasI GACNNNNNNGTC 1 cut(s) 1532
Acc36I ACCTGC 1 cut(s) 515
Acc65I GGTACC 1 cut(s) 1110
AccB1I GGYRCC 2 cut(s) 380, 1110
AccI GTMKAC 2 cut(s) 753, 1158
AccII CGCG 2 cut(s) 47, 96
AciI CCGC 7 cut(s) 47, 115, 173, 219, 296, 384, 626
AclWI GGATC 1 cut(s) 771
AcoI YGGCCR 1 cut(s) 1299
AcsI RAATTY 1 cut(s) 1226
AcuI CTGAAG 2 cut(s) 454, 1560
AcvI CACGTG 1 cut(s) 632
AcyI GRCGYC 1 cut(s) 170
AfaI GTAC 3 cut(s) 818, 1112, 1183
AfiI CCNNNNNNNGG 2 cut(s) 575, 1237
AflIII ACRYGT 1 cut(s) 631
AgsI TTSAA 5 cut(s) 694, 1124, 1144, 1237, 1548
AjiI CACGTC 1 cut(s) 247
AjnI CCWGG 1 cut(s) 138
AjuI GAANNNNNNNTTGG 4 cut(s) 1069, 1101, 1454, 1486
AleI CACNNNNGTG 1 cut(s) 32
AluBI AGCT 6 cut(s) 78, 121, 350, 372, 410, 938
AluI AGCT 6 cut(s) 78, 121, 350, 372, 410, 938
Alw21I GWGCWC 3 cut(s) 200, 252, 750
Alw26I GTCTC 4 cut(s) 144, 502, 904, 1135
AlwI GGATC 1 cut(s) 771
AlwNI CAGNNNCTG 1 cut(s) 825
AoxI GGCC 4 cut(s) 177, 577, 678, 1299
ApeKI GCWGC 2 cut(s) 426, 1095
ApoI RAATTY 1 cut(s) 1226
Asp700I GAANNNNTTC 1 cut(s) 467
Asp718I GGTACC 1 cut(s) 1110
AspLEI GCGC 3 cut(s) 47, 191, 719
AspS9I GGNCC 1 cut(s) 578
AsuHPI GGTGA 2 cut(s) 834, 1580
AsuII TTCGAA 2 cut(s) 224, 461
BanI GGYRCC 2 cut(s) 380, 1110
BbrPI CACGTG 1 cut(s) 632
Bbv12I GWGCWC 3 cut(s) 200, 252, 750
BbvI GCAGC 2 cut(s) 438, 1082
BccI CCATC 3 cut(s) 284, 644, 844
BceAI ACGGC 2 cut(s) 58, 75
BcgI CGANNNNNNTGC 2 cut(s) 539, 573
BciT130I CCWGG 1 cut(s) 140
BciVI GTATCC 2 cut(s) 630, 1396
BcoDI GTCTC 4 cut(s) 144, 502, 904, 1135
BfaI CTAG 1 cut(s) 1259
BfmI CTRYAG 1 cut(s) 933
BfoI RGCGCY 1 cut(s) 720
BfuAI ACCTGC 1 cut(s) 515
BfuI GTATCC 2 cut(s) 630, 1396
BisI GCNGC 6 cut(s) 48, 173, 219, 384, 427, 1096
BlsI GCNGC 6 cut(s) 49, 174, 220, 385, 428, 1097
Bme1390I CCNGG 1 cut(s) 140
BmgBI CACGTC 1 cut(s) 247
BmgT120I GGNCC 1 cut(s) 578
BmiI GGNNCC 3 cut(s) 162, 382, 1112
BmrFI CCNGG 1 cut(s) 140
BmrI ACTGGG 1 cut(s) 1361
BmsI GCATC 7 cut(s) 513, 672, 693, 799, 1009, 1279, 1360
BmuI ACTGGG 1 cut(s) 1361
BoxI GACNNNNGTC 1 cut(s) 90
BpmI CTGGAG 2 cut(s) 305, 484
Bpu10I CCTNAGC 1 cut(s) 340
Bpu14I TTCGAA 2 cut(s) 224, 461
BpuEI CTTGAG 1 cut(s) 1296
BsaAI YACGTR 1 cut(s) 632
BsaHI GRCGYC 1 cut(s) 170
BsaI GGTCTC 1 cut(s) 144
BsaJI CCNNGG 4 cut(s) 33, 712, 729, 1194
BsaWI WCCGGW 1 cut(s) 1107
Bsc4I CCNNNNNNNGG 2 cut(s) 575, 1237
Bse1I ACTGG 4 cut(s) 322, 452, 1356, 1501
BseBI CCWGG 1 cut(s) 140
BseDI CCNNGG 4 cut(s) 33, 712, 729, 1194
BseGI GGATG 3 cut(s) 282, 362, 1375
BseLI CCNNNNNNNGG 2 cut(s) 575, 1237
BseMII CTCAG 1 cut(s) 1433
BseNI ACTGG 4 cut(s) 322, 452, 1356, 1501
BseRI GAGGAG 2 cut(s) 153, 190
BseXI GCAGC 2 cut(s) 438, 1082
BseYI CCCAGC 1 cut(s) 346
BsgI GTGCAG 2 cut(s) 373, 761
Bsh1236I CGCG 2 cut(s) 47, 96
BshFI GGCC 4 cut(s) 179, 579, 680, 1301
BshNI GGYRCC 2 cut(s) 380, 1110
BsiHKAI GWGCWC 3 cut(s) 200, 252, 750
BsiSI CCGG 1 cut(s) 1108
BslFI GGGAC 1 cut(s) 278
BslI CCNNNNNNNGG 2 cut(s) 575, 1237
BsmAI GTCTC 4 cut(s) 144, 502, 904, 1135
BsmFI GGGAC 1 cut(s) 278
BsnI GGCC 4 cut(s) 179, 579, 680, 1301
Bso31I GGTCTC 1 cut(s) 144
Bsp119I TTCGAA 2 cut(s) 224, 461
Bsp1286I GDGCHC 3 cut(s) 200, 252, 750
Bsp1407I TGTACA 1 cut(s) 816
Bsp143I GATC 5 cut(s) 213, 763, 871, 1338, 1495
Bsp68I TCGCGA 1 cut(s) 96
BspACI CCGC 7 cut(s) 47, 115, 173, 219, 296, 384, 626
BspANI GGCC 4 cut(s) 179, 579, 680, 1301
BspCNI CTCAG 1 cut(s) 1432
BspFNI CGCG 2 cut(s) 47, 96
BspHI TCATGA 1 cut(s) 924
BspLI GGNNCC 3 cut(s) 162, 382, 1112
BspMI ACCTGC 1 cut(s) 515
BspPI GGATC 1 cut(s) 771
BspQI GCTCTTC 1 cut(s) 357
BspT104I TTCGAA 2 cut(s) 224, 461
BspT107I GGYRCC 2 cut(s) 380, 1110
BspTNI GGTCTC 1 cut(s) 144
BsrGI TGTACA 1 cut(s) 816
BsrI ACTGG 4 cut(s) 322, 452, 1356, 1501
BssECI CCNNGG 4 cut(s) 33, 712, 729, 1194
BssMI GATC 5 cut(s) 213, 763, 871, 1338, 1495
BssNAI GTATAC 1 cut(s) 1159
BssNI GRCGYC 1 cut(s) 170
BssT1I CCWWGG 2 cut(s) 712, 1194
Bst1107I GTATAC 1 cut(s) 1159
Bst2UI CCWGG 1 cut(s) 140
Bst4CI ACNGT 5 cut(s) 34, 130, 380, 1062, 1186
Bst6I CTCTTC 3 cut(s) 357, 1168, 1236
BstACI GRCGYC 1 cut(s) 170
BstAPI GCANNNNNTGC 2 cut(s) 435, 1213
BstAUI TGTACA 1 cut(s) 816
BstBAI YACGTR 1 cut(s) 632
BstBI TTCGAA 2 cut(s) 224, 461
BstC8I GCNNGC 5 cut(s) 119, 177, 300, 388, 1332
BstDEI CTNAG 4 cut(s) 340, 769, 1419, 1592
BstDSI CCRYGG 1 cut(s) 33
BstF5I GGATG 3 cut(s) 282, 362, 1375
BstFNI CGCG 2 cut(s) 47, 96
BstH2I RGCGCY 1 cut(s) 720
BstHHI GCGC 3 cut(s) 47, 191, 719
BstKTI GATC 5 cut(s) 216, 766, 874, 1341, 1498
BstMAI GTCTC 4 cut(s) 144, 502, 904, 1135
BstMBI GATC 5 cut(s) 213, 763, 871, 1338, 1495
BstMWI GCNNNNNNNGC 4 cut(s) 195, 435, 1213, 1298
BstNI CCWGG 1 cut(s) 140
BstNSI RCATGY 1 cut(s) 1207
BstPAI GACNNNNGTC 1 cut(s) 90
BstSCI CCNGG 1 cut(s) 138
BstSFI CTRYAG 1 cut(s) 933
BstUI CGCG 2 cut(s) 47, 96
BstV1I GCAGC 2 cut(s) 438, 1082
BstX2I RGATCY 1 cut(s) 763
BstXI CCANNNNNNTGG 1 cut(s) 1432
BstYI RGATCY 1 cut(s) 763
BstZ17I GTATAC 1 cut(s) 1159
BsuI GTATCC 2 cut(s) 630, 1396
BsuRI GGCC 4 cut(s) 179, 579, 680, 1301
BtgI CCRYGG 1 cut(s) 33
BtrI CACGTC 1 cut(s) 247
BtsCI GGATG 3 cut(s) 282, 362, 1375
BtsI GCAGTG 1 cut(s) 545
BtsIMutI CAGTG 3 cut(s) 545, 1508, 1536
BtuMI TCGCGA 1 cut(s) 96
BveI ACCTGC 1 cut(s) 515
Cac8I GCNNGC 5 cut(s) 119, 177, 300, 388, 1332
CaiI CAGNNNCTG 1 cut(s) 825
CciI TCATGA 1 cut(s) 924
CfoI GCGC 3 cut(s) 47, 191, 719
Cfr13I GGNCC 1 cut(s) 578
CseI GACGC 2 cut(s) 178, 427
Csp6I GTAC 3 cut(s) 817, 1111, 1182
CviAII CATG 6 cut(s) 133, 682, 925, 1204, 1321, 1382
CviQI GTAC 3 cut(s) 817, 1111, 1182
DdeI CTNAG 4 cut(s) 340, 769, 1419, 1592
DpnI GATC 5 cut(s) 215, 765, 873, 1340, 1497
DpnII GATC 5 cut(s) 213, 763, 871, 1338, 1495
DrdI GACNNNNNNGTC 1 cut(s) 1532
DseDI GACNNNNNNGTC 1 cut(s) 1532
EaeI YGGCCR 1 cut(s) 1299
Eam1104I CTCTTC 3 cut(s) 357, 1168, 1236
EarI CTCTTC 3 cut(s) 357, 1168, 1236
EciI GGCGGA 1 cut(s) 615
Eco130I CCWWGG 2 cut(s) 712, 1194
Eco31I GGTCTC 1 cut(s) 144
Eco57I CTGAAG 2 cut(s) 454, 1560
Eco72I CACGTG 1 cut(s) 632
EcoRI GAATTC 1 cut(s) 1226
EcoRII CCWGG 1 cut(s) 138
EcoT14I CCWWGG 2 cut(s) 712, 1194
EcoT22I ATGCAT 2 cut(s) 407, 687
ErhI CCWWGG 2 cut(s) 712, 1194
FaeI CATG 6 cut(s) 136, 685, 928, 1207, 1324, 1385
FaqI GGGAC 1 cut(s) 278
FatI CATG 6 cut(s) 132, 681, 924, 1203, 1320, 1381
FauI CCCGC 1 cut(s) 303
FauNDI CATATG 1 cut(s) 1513
FblI GTMKAC 2 cut(s) 753, 1158
Fnu4HI GCNGC 6 cut(s) 48, 173, 219, 384, 427, 1096
FokI GGATG 3 cut(s) 269, 349, 1382
Fsp4HI GCNGC 6 cut(s) 48, 173, 219, 384, 427, 1096
FspBI CTAG 1 cut(s) 1259
GlaI GCGC 3 cut(s) 46, 190, 718
GluI GCNGC 6 cut(s) 48, 173, 219, 384, 427, 1096
GsaI CCCAGC 1 cut(s) 350
GsuI CTGGAG 2 cut(s) 305, 484
HaeII RGCGCY 1 cut(s) 720
HaeIII GGCC 4 cut(s) 179, 579, 680, 1301
HapII CCGG 1 cut(s) 1108
HgaI GACGC 2 cut(s) 178, 427
HhaI GCGC 3 cut(s) 47, 191, 719
Hin1I GRCGYC 1 cut(s) 170
Hin1II CATG 6 cut(s) 136, 685, 928, 1207, 1324, 1385
Hin6I GCGC 3 cut(s) 45, 189, 717
HinP1I GCGC 3 cut(s) 45, 189, 717
HincII GTYRAC 3 cut(s) 442, 726, 853
HindII GTYRAC 3 cut(s) 442, 726, 853
HindIII AAGCTT 1 cut(s) 370
HinfI GANTC 8 cut(s) 486, 638, 694, 798, 822, 1037, 1252, 1517
HpaII CCGG 1 cut(s) 1108
HphI GGTGA 2 cut(s) 834, 1580
Hpy166II GTNNAC 7 cut(s) 37, 442, 538, 726, 754, 853, 1159
Hpy188I TCNGA 6 cut(s) 85, 149, 168, 241, 485, 1495
Hpy188III TCNNGA 8 cut(s) 70, 95, 206, 539, 925, 1189, 1259, 1313
Hpy8I GTNNAC 7 cut(s) 37, 442, 538, 726, 754, 853, 1159
Hpy99I CGWCG 5 cut(s) 44, 96, 172, 380, 1449
HpyAV CCTTC 2 cut(s) 246, 478
HpyCH4III ACNGT 5 cut(s) 34, 130, 380, 1062, 1186
HpyCH4IV ACGT 3 cut(s) 194, 246, 631
HpyF10VI GCNNNNNNNGC 4 cut(s) 195, 435, 1213, 1298
HpyF3I CTNAG 4 cut(s) 340, 769, 1419, 1592
HpySE526I ACGT 3 cut(s) 194, 246, 631
Hsp92I GRCGYC 1 cut(s) 170
Hsp92II CATG 6 cut(s) 136, 685, 928, 1207, 1324, 1385
HspAI GCGC 3 cut(s) 45, 189, 717
KpnI GGTACC 1 cut(s) 1114
Kzo9I GATC 5 cut(s) 213, 763, 871, 1338, 1495
LguI GCTCTTC 1 cut(s) 357
LmnI GCTCC 3 cut(s) 166, 203, 255
Lsp1109I GCAGC 2 cut(s) 438, 1082
LweI GCATC 7 cut(s) 513, 672, 693, 799, 1009, 1279, 1360
MaeI CTAG 1 cut(s) 1259
MaeII ACGT 3 cut(s) 194, 246, 631
MaeIII GTNAC 6 cut(s) 450, 790, 1080, 1534, 1563, 1595
MalI GATC 5 cut(s) 215, 765, 873, 1340, 1497
MboI GATC 5 cut(s) 213, 763, 871, 1338, 1495
MboII GAAGA 5 cut(s) 344, 351, 1155, 1247, 1253
MfeI CAATTG 1 cut(s) 413
MflI RGATCY 1 cut(s) 763
MhlI GDGCHC 3 cut(s) 200, 252, 750
MlyI GAGTC 3 cut(s) 792, 1031, 1511
MmeI TCCRAC 1 cut(s) 191
Mph1103I ATGCAT 2 cut(s) 407, 687
MroXI GAANNNNTTC 1 cut(s) 467
MseI TTAA 7 cut(s) 399, 608, 657, 999, 1248, 1605, 1619
MslI CAYNNNNRTG 2 cut(s) 32, 786
MspA1I CMGCKG 1 cut(s) 175
MspI CCGG 1 cut(s) 1108
MspR9I CCNGG 1 cut(s) 140
MunI CAATTG 1 cut(s) 413
MvaI CCWGG 1 cut(s) 140
MvnI CGCG 2 cut(s) 47, 96
MwoI GCNNNNNNNGC 4 cut(s) 195, 435, 1213, 1298
NdeI CATATG 1 cut(s) 1513
NdeII GATC 5 cut(s) 213, 763, 871, 1338, 1495
NlaIII CATG 6 cut(s) 136, 685, 928, 1207, 1324, 1385
NlaIV GGNNCC 3 cut(s) 162, 382, 1112
NmuCI GTSAC 4 cut(s) 450, 1534, 1563, 1595
NruI TCGCGA 1 cut(s) 96
NsiI ATGCAT 2 cut(s) 407, 687
NspI RCATGY 1 cut(s) 1207
NspV TTCGAA 2 cut(s) 224, 461
OliI CACNNNNGTG 1 cut(s) 32
PagI TCATGA 1 cut(s) 924
PciSI GCTCTTC 1 cut(s) 357
PdmI GAANNNNTTC 1 cut(s) 467
PfeI GAWTC 5 cut(s) 486, 638, 694, 822, 1252
PkrI GCNGC 6 cut(s) 49, 174, 220, 385, 428, 1097
PleI GAGTC 3 cut(s) 792, 1031, 1511
PmaCI CACGTG 1 cut(s) 632
PmlI CACGTG 1 cut(s) 632
PpsI GAGTC 3 cut(s) 792, 1031, 1511
Ppu21I YACGTR 1 cut(s) 632
PshAI GACNNNNGTC 1 cut(s) 90
PsiI TTATAA 1 cut(s) 1630
Psp6I CCWGG 1 cut(s) 138
PspCI CACGTG 1 cut(s) 632
PspFI CCCAGC 1 cut(s) 346
PspGI CCWGG 1 cut(s) 138
PspN4I GGNNCC 3 cut(s) 162, 382, 1112
PspPI GGNCC 1 cut(s) 578
PstNI CAGNNNCTG 1 cut(s) 825
PsuI RGATCY 1 cut(s) 763
RruI TCGCGA 1 cut(s) 96
RsaI GTAC 3 cut(s) 818, 1112, 1183
RsaNI GTAC 3 cut(s) 817, 1111, 1182
RseI CAYNNNNRTG 2 cut(s) 32, 786
SapI GCTCTTC 1 cut(s) 357
SaqAI TTAA 7 cut(s) 399, 608, 657, 999, 1248, 1605, 1619
SatI GCNGC 6 cut(s) 48, 173, 219, 384, 427, 1096
Sau3AI GATC 5 cut(s) 213, 763, 871, 1338, 1495
Sau96I GGNCC 1 cut(s) 578
SchI GAGTC 3 cut(s) 792, 1031, 1511
ScrFI CCNGG 1 cut(s) 140
SduI GDGCHC 3 cut(s) 200, 252, 750
SfaNI GCATC 7 cut(s) 513, 672, 693, 799, 1009, 1279, 1360
SfcI CTRYAG 1 cut(s) 933
SfuI TTCGAA 2 cut(s) 224, 461
SmiMI CAYNNNNRTG 2 cut(s) 32, 786
SmlI CTYRAG 1 cut(s) 1311
SmoI CTYRAG 1 cut(s) 1311
SsiI CCGC 7 cut(s) 47, 115, 173, 219, 296, 384, 626
SspMI CTAG 1 cut(s) 1259
StyD4I CCNGG 1 cut(s) 138
StyI CCWWGG 2 cut(s) 712, 1194
TaaI ACNGT 5 cut(s) 34, 130, 380, 1062, 1186
TaiI ACGT 3 cut(s) 197, 249, 634
TatI WGTACW 1 cut(s) 816
TauI GCSGC 4 cut(s) 50, 175, 221, 386
TfiI GAWTC 5 cut(s) 486, 638, 694, 822, 1252
Tru1I TTAA 7 cut(s) 399, 608, 657, 999, 1248, 1605, 1619
Tru9I TTAA 7 cut(s) 399, 608, 657, 999, 1248, 1605, 1619
TscAI CASTG 3 cut(s) 552, 1508, 1543
TseFI GTSAC 4 cut(s) 450, 1534, 1563, 1595
TseI GCWGC 2 cut(s) 426, 1095
Tsp45I GTSAC 4 cut(s) 450, 1534, 1563, 1595
TspDTI ATGAA 5 cut(s) 351, 626, 941, 1475, 1605
TspGWI ACGGA 1 cut(s) 1480
TspRI CASTG 3 cut(s) 552, 1508, 1543
XapI RAATTY 1 cut(s) 1226
XbaI TCTAGA 1 cut(s) 1258
XceI RCATGY 1 cut(s) 1207
XcmI CCANNNNNNNNNTGG 1 cut(s) 31
XmiI GTMKAC 2 cut(s) 753, 1158
XmnI GAANNNNTTC 1 cut(s) 467
XspI CTAG 1 cut(s) 1259
Zsp2I ATGCAT 2 cut(s) 407, 687
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.