Rh2BG262700

RRP12-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0000835
Physical Location & Seq
Forward (+)
28111266 .. 28114058
2793 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG262700.1

Sequence Viewer

Length: 516 bp
ATGGAGGTTGAGGACTGCGACTTCAACATGGGCAGCTGGATCCTGATGCGCTTCACCAATCCGACGCGCAAGGATCACCTAGAGATTCGCCAAATCACAGTCGCCATGGCCCATGACTTCATAGCGCAGAAGGTGAGGAAACCATCATCTCATGTGATTGGGGAGGTGCTGCAAAGTTTGCAAGGCACACCGCATCTTGCCCCTGCAAGTGAAGCCCTTACTAGCTTGTTTGAAAGGTGGTGGCTTCCTGTGCTTTCTGGTGGTGGCGCATCAAATACTGCTGCTGCTGCTGCCTTGACTCGGCGAGGAGTCCCAGAGTACTGGTATCTTTTGGATGTTATGAAAATATGTCTCGTCTTCATGCCTACCAAGTTGAAGCTAGCAGTTCTCAACTACTTTAAGGCTATCTTCGAACTCATCTTGGAACTGGAAGCGCATAGACCTCCTGTTACGGTGCGCCTAGAGTGGTGCCTTCAAAGAGTCTGCCTCCACCCATCTACAGATGTTCCACCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NUC173

Protein Analysis

171

Amino Acids

19.37

Weight (kDa)

7.01

Isoelectric Point (pI)

45.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HEAT_RRP12_N PF25772 43 - 169 2e-17 RRP12-like, N-terminal HEAT repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 468
AccII CGCG 1 cut(s) 67
AciI CCGC 1 cut(s) 191
AclWI GGATC 3 cut(s) 34, 47, 81
AfaI GTAC 1 cut(s) 320
AfiI CCNNNNNNNGG 1 cut(s) 300
AgsI TTSAA 4 cut(s) 25, 233, 376, 476
AluBI AGCT 3 cut(s) 36, 225, 379
AluI AGCT 3 cut(s) 36, 225, 379
Alw26I GTCTC 1 cut(s) 356
AlwI GGATC 3 cut(s) 34, 47, 81
AoxI GGCC 1 cut(s) 108
ApeKI GCWGC 6 cut(s) 33, 169, 281, 284, 287, 290
AspLEI GCGC 6 cut(s) 51, 69, 127, 269, 436, 459
AspS9I GGNCC 1 cut(s) 109
AsuHPI GGTGA 3 cut(s) 46, 68, 145
AsuII TTCGAA 1 cut(s) 411
AsuNHI GCTAGC 1 cut(s) 379
BamHI GGATCC 1 cut(s) 39
BanI GGYRCC 1 cut(s) 468
BbsI GAAGAC 1 cut(s) 349
BbvI GCAGC 6 cut(s) 45, 156, 268, 271, 274, 277
BccI CCATC 2 cut(s) 151, 502
BcoDI GTCTC 1 cut(s) 356
BfaI CTAG 4 cut(s) 80, 222, 380, 461
BfmI CTRYAG 1 cut(s) 498
BisI GCNGC 6 cut(s) 34, 170, 282, 285, 288, 291
BlsI GCNGC 6 cut(s) 35, 171, 283, 286, 289, 292
BmcAI AGTACT 1 cut(s) 320
BmgT120I GGNCC 1 cut(s) 109
BmiI GGNNCC 2 cut(s) 41, 470
BmsI GCATC 3 cut(s) 36, 202, 278
BmtI GCTAGC 1 cut(s) 383
BpiI GAAGAC 1 cut(s) 349
BplI GAGNNNNNCTC 2 cut(s) 471, 503
Bpu14I TTCGAA 1 cut(s) 411
BsaJI CCNNGG 1 cut(s) 105
Bsc4I CCNNNNNNNGG 1 cut(s) 300
Bse1I ACTGG 2 cut(s) 326, 432
BseDI CCNNGG 1 cut(s) 105
BseGI GGATG 1 cut(s) 340
BseLI CCNNNNNNNGG 1 cut(s) 300
BseNI ACTGG 2 cut(s) 326, 432
BseRI GAGGAG 1 cut(s) 321
BseXI GCAGC 6 cut(s) 45, 156, 268, 271, 274, 277
Bsh1236I CGCG 1 cut(s) 67
BshFI GGCC 1 cut(s) 110
BshNI GGYRCC 1 cut(s) 468
BslFI GGGAC 1 cut(s) 296
BslI CCNNNNNNNGG 1 cut(s) 300
BsmAI GTCTC 1 cut(s) 356
BsmFI GGGAC 1 cut(s) 296
BsnI GGCC 1 cut(s) 110
Bsp119I TTCGAA 1 cut(s) 411
Bsp143I GATC 2 cut(s) 39, 73
Bsp19I CCATGG 1 cut(s) 105
BspACI CCGC 1 cut(s) 191
BspANI GGCC 1 cut(s) 110
BspFNI CGCG 1 cut(s) 67
BspLI GGNNCC 2 cut(s) 41, 470
BspOI GCTAGC 1 cut(s) 383
BspPI GGATC 3 cut(s) 34, 47, 81
BspT104I TTCGAA 1 cut(s) 411
BspT107I GGYRCC 1 cut(s) 468
BsrI ACTGG 2 cut(s) 326, 432
BssECI CCNNGG 1 cut(s) 105
BssMI GATC 2 cut(s) 39, 73
BssT1I CCWWGG 1 cut(s) 105
Bst4CI ACNGT 2 cut(s) 100, 454
BstAPI GCANNNNNTGC 1 cut(s) 178
BstBI TTCGAA 1 cut(s) 411
BstC8I GCNNGC 1 cut(s) 381
BstDSI CCRYGG 1 cut(s) 105
BstF5I GGATG 1 cut(s) 340
BstFNI CGCG 1 cut(s) 67
BstHHI GCGC 6 cut(s) 51, 69, 127, 269, 436, 459
BstKTI GATC 2 cut(s) 42, 76
BstMAI GTCTC 1 cut(s) 356
BstMBI GATC 2 cut(s) 39, 73
BstMWI GCNNNNNNNGC 5 cut(s) 178, 212, 250, 287, 290
BstSFI CTRYAG 1 cut(s) 498
BstUI CGCG 1 cut(s) 67
BstV1I GCAGC 6 cut(s) 45, 156, 268, 271, 274, 277
BstV2I GAAGAC 1 cut(s) 349
BstX2I RGATCY 1 cut(s) 39
BstXI CCANNNNNNTGG 1 cut(s) 321
BstYI RGATCY 1 cut(s) 39
BsuRI GGCC 1 cut(s) 110
BtgI CCRYGG 1 cut(s) 105
BtsCI GGATG 1 cut(s) 340
Cac8I GCNNGC 1 cut(s) 381
CfoI GCGC 6 cut(s) 51, 69, 127, 269, 436, 459
Cfr13I GGNCC 1 cut(s) 109
CseI GACGC 1 cut(s) 73
Csp6I GTAC 1 cut(s) 319
CviAII CATG 5 cut(s) 28, 106, 113, 152, 361
CviJI RGCY 7 cut(s) 36, 110, 215, 225, 244, 379, 404
CviKI_1 RGCY 7 cut(s) 36, 110, 215, 225, 244, 379, 404
CviQI GTAC 1 cut(s) 319
DpnI GATC 2 cut(s) 41, 75
DpnII GATC 2 cut(s) 39, 73
Eco130I CCWWGG 1 cut(s) 105
EcoT14I CCWWGG 1 cut(s) 105
ErhI CCWWGG 1 cut(s) 105
FaeI CATG 5 cut(s) 31, 109, 116, 155, 364
FalI AAGNNNNNCTT 2 cut(s) 392, 424
FaqI GGGAC 1 cut(s) 296
FatI CATG 5 cut(s) 27, 105, 112, 151, 360
Fnu4HI GCNGC 6 cut(s) 34, 170, 282, 285, 288, 291
FokI GGATG 1 cut(s) 347
Fsp4HI GCNGC 6 cut(s) 34, 170, 282, 285, 288, 291
FspBI CTAG 4 cut(s) 80, 222, 380, 461
GlaI GCGC 6 cut(s) 50, 68, 126, 268, 435, 458
GluI GCNGC 6 cut(s) 34, 170, 282, 285, 288, 291
HaeIII GGCC 1 cut(s) 110
HgaI GACGC 1 cut(s) 73
HhaI GCGC 6 cut(s) 51, 69, 127, 269, 436, 459
Hin1II CATG 5 cut(s) 31, 109, 116, 155, 364
Hin6I GCGC 6 cut(s) 49, 67, 125, 267, 434, 457
HinP1I GCGC 6 cut(s) 49, 67, 125, 267, 434, 457
HinfI GANTC 4 cut(s) 85, 298, 309, 480
HphI GGTGA 3 cut(s) 46, 68, 145
Hpy188I TCNGA 1 cut(s) 63
Hpy188III TCNNGA 1 cut(s) 43
Hpy99I CGWCG 1 cut(s) 67
HpyAV CCTTC 2 cut(s) 124, 482
HpyCH4III ACNGT 2 cut(s) 100, 454
HpyCH4V TGCA 3 cut(s) 172, 181, 206
HpyF10VI GCNNNNNNNGC 5 cut(s) 178, 212, 250, 287, 290
Hsp92II CATG 5 cut(s) 31, 109, 116, 155, 364
HspAI GCGC 6 cut(s) 49, 67, 125, 267, 434, 457
Kzo9I GATC 2 cut(s) 39, 73
LpnPI CCDG 9 cut(s) 22, 56, 216, 243, 261, 307, 327, 413, 459
Lsp1109I GCAGC 6 cut(s) 45, 156, 268, 271, 274, 277
LweI GCATC 3 cut(s) 36, 202, 278
MaeI CTAG 4 cut(s) 80, 222, 380, 461
MaeIII GTNAC 1 cut(s) 448
MalI GATC 2 cut(s) 41, 75
MboI GATC 2 cut(s) 39, 73
MboII GAAGA 2 cut(s) 349, 400
MflI RGATCY 1 cut(s) 39
MlyI GAGTC 3 cut(s) 292, 318, 489
MmeI TCCRAC 1 cut(s) 86
MnlI CCTC 6 cut(s) 4, 129, 157, 299, 453, 497
MseI TTAA 1 cut(s) 399
MspA1I CMGCKG 1 cut(s) 36
MvnI CGCG 1 cut(s) 67
MwoI GCNNNNNNNGC 5 cut(s) 178, 212, 250, 287, 290
NcoI CCATGG 1 cut(s) 105
NdeII GATC 2 cut(s) 39, 73
NheI GCTAGC 1 cut(s) 379
NlaIII CATG 5 cut(s) 31, 109, 116, 155, 364
NlaIV GGNNCC 2 cut(s) 41, 470
NmeAIII GCCGAG 1 cut(s) 280
NspV TTCGAA 1 cut(s) 411
PfeI GAWTC 1 cut(s) 85
PkrI GCNGC 6 cut(s) 35, 171, 283, 286, 289, 292
PleI GAGTC 3 cut(s) 292, 317, 488
PpsI GAGTC 3 cut(s) 292, 317, 488
PspN4I GGNNCC 2 cut(s) 41, 470
PspPI GGNCC 1 cut(s) 109
PsuI RGATCY 1 cut(s) 39
PvuII CAGCTG 1 cut(s) 36
RsaI GTAC 1 cut(s) 320
RsaNI GTAC 1 cut(s) 319
SaqAI TTAA 1 cut(s) 399
SatI GCNGC 6 cut(s) 34, 170, 282, 285, 288, 291
Sau3AI GATC 2 cut(s) 39, 73
Sau96I GGNCC 1 cut(s) 109
ScaI AGTACT 1 cut(s) 320
SchI GAGTC 3 cut(s) 292, 318, 489
SetI ASST 9 cut(s) 9, 38, 81, 135, 168, 227, 239, 381, 445
SfaNI GCATC 3 cut(s) 36, 202, 278
SfcI CTRYAG 1 cut(s) 498
SfuI TTCGAA 1 cut(s) 411
SsiI CCGC 1 cut(s) 191
SspMI CTAG 4 cut(s) 80, 222, 380, 461
StyI CCWWGG 1 cut(s) 105
TaaI ACNGT 2 cut(s) 100, 454
TaqI TCGA 1 cut(s) 411
TatI WGTACW 1 cut(s) 318
TfiI GAWTC 1 cut(s) 85
Tru1I TTAA 1 cut(s) 399
Tru9I TTAA 1 cut(s) 399
TseI GCWGC 6 cut(s) 33, 169, 281, 284, 287, 290
TspDTI ATGAA 3 cut(s) 109, 349, 356
XspI CTAG 4 cut(s) 80, 222, 380, 461
ZrmI AGTACT 1 cut(s) 320
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.