Rroxscaffold_2G00130540

RRP12-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000835
Physical Location & Seq
Forward (+)
66755625 .. 66756909
1285 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00130540.1

Sequence Viewer

Length: 1062 bp
ATGGATACCCTACTCGCTCCGAATTCCAACTCCAACTACGACGACTTGAGCATCAGCAGTGCGATCCTGATGCGCTTCACCGATCCGACGCGCAAGGAGCACCTAGAAATCCGCAAAATCATAGTAGGAATGGCCCATGATCAGAAGGTGGCTCCAACGGCCGTAGCTTACTTGAAGGCCACGGGATCGTTTCTCTACCGGATCTCATCCTCTTCCGAAACAAAACCTGATCCGATGTTCCACCACAGCTGTTGCTCGACCTCTTATGCTTGGTATCCAATTCTGTCTCTAGACAAGTTCCGCACGCGCATGCATGAGGGGGCAACCACGTCCTCATTGCTCAACAGTGGAGTGGCAAAAGTTTATTCCTCCAACAGGGGAATCTGTGTGAGTAAACTCCCTCTTGTGTTCGATGCACTCAAAATTTTGATGGGGAAACCTGTACAAGAGGACGACGGCCATATAGAAGAGGATATTGATGCCGCAGCACACGCAATGAAGTGTCTTATTCATGCTTTCATTGATCAAGGCTTGATCGAACAGGAGCAACCTATAGTCGAAAAGCTGTGTTCTACTGTTCAAAGCTTACTTGGTTATCACCGCACAAATGCTCCTGCTCTGAGCATGGACCTTGCATTTGAAGTTGTTTCAACAATGTTTGATAAATTAGGGGCATACGCTTCTTACTTCATGAGGGGCACGCTGGAGAACTTGGCAGACATGCACAAGTTGCCTGATGAAGACTTCCCGTTTAGGAAGCAGGCCACTCTTCTTCTTGGATCGCTTGTCGAGGAGAAATGTGGGCTAAATGATGTTAATATTACTGCTGTCCTGCCTCAAGGACAACTCAGGGAACTGCTTGCAGATTTTAGGGAGCTTAAATGCATATCAAGGTGGGAACTTTCTCATCGAAAACGGAAGATGGGATTAAATATTGAGCCCTTTCTAGGAAGGGACTGGTCAGAGGACATGAGGGCTGAGGAAGCTACAATTACAGCCATATCTGGTGCAGCGCAAATGACAAAGAAGATTAAACTCAATAACCAGGCTTCCTTAACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NUC173

Protein Analysis

353

Amino Acids

39.41

Weight (kDa)

6.14

Isoelectric Point (pI)

42.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRP12_HEAT PF08161 160 - 264 1.3e-10 Ribosomal RNA-processing protein 12, HEAT repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 91, 307
AciI CCGC 4 cut(s) 112, 301, 483, 601
AclWI GGATC 6 cut(s) 58, 77, 193, 209, 224, 787
AcoI YGGCCR 2 cut(s) 159, 457
AcsI RAATTY 2 cut(s) 22, 423
AfaI GTAC 1 cut(s) 444
AfiI CCNNNNNNNGG 2 cut(s) 375, 947
AgsI TTSAA 4 cut(s) 175, 581, 641, 651
AjiI CACGTC 1 cut(s) 330
AjnI CCWGG 1 cut(s) 1044
AluBI AGCT 6 cut(s) 167, 249, 565, 585, 877, 986
AluI AGCT 6 cut(s) 167, 249, 565, 585, 877, 986
Alw21I GWGCWC 1 cut(s) 102
Alw26I GTCTC 1 cut(s) 291
AlwI GGATC 6 cut(s) 58, 77, 193, 209, 224, 787
AoxI GGCC 5 cut(s) 132, 159, 177, 457, 762
ApeKI GCWGC 2 cut(s) 485, 1010
ApoI RAATTY 2 cut(s) 22, 423
ArsI GACNNNNNNTTYG 2 cut(s) 620, 652
AspLEI GCGC 4 cut(s) 75, 93, 309, 1015
AspS9I GGNCC 2 cut(s) 133, 628
AsuHPI GGTGA 2 cut(s) 70, 590
AvaII GGWCC 1 cut(s) 628
BaeGI GKGCMC 1 cut(s) 701
BanII GRGCYC 1 cut(s) 942
BbsI GAAGAC 1 cut(s) 747
Bbv12I GWGCWC 1 cut(s) 102
BbvCI CCTCAGC 1 cut(s) 978
BbvI GCAGC 2 cut(s) 497, 1022
BccI CCATC 2 cut(s) 424, 916
BceAI ACGGC 3 cut(s) 146, 174, 472
BcgI CGANNNNNNTGC 2 cut(s) 52, 86
BciT130I CCWGG 1 cut(s) 1046
BciVI GTATCC 1 cut(s) 285
BclI TGATCA 2 cut(s) 139, 523
BcoDI GTCTC 1 cut(s) 291
BfaI CTAG 3 cut(s) 104, 290, 947
BfmI CTRYAG 1 cut(s) 552
BfuI GTATCC 1 cut(s) 285
BisI GCNGC 3 cut(s) 483, 486, 1011
BlsI GCNGC 3 cut(s) 484, 487, 1012
Bme1390I CCNGG 1 cut(s) 1046
Bme18I GGWCC 1 cut(s) 628
BmgBI CACGTC 1 cut(s) 330
BmgT120I GGNCC 2 cut(s) 133, 628
BmiI GGNNCC 1 cut(s) 153
BmrFI CCNGG 1 cut(s) 1046
BmsI GCATC 4 cut(s) 60, 60, 403, 469
BpiI GAAGAC 1 cut(s) 747
BpmI CTGGAG 1 cut(s) 725
Bpu10I CCTNAGC 1 cut(s) 978
BpuEI CTTGAG 2 cut(s) 67, 822
BsaJI CCNNGG 1 cut(s) 180
BsaWI WCCGGW 1 cut(s) 198
BsaXI ACNNNNNCTCC 2 cut(s) 595, 625
Bsc4I CCNNNNNNNGG 2 cut(s) 375, 947
Bse1I ACTGG 1 cut(s) 962
Bse3DI GCAATG 2 cut(s) 335, 501
BseBI CCWGG 1 cut(s) 1046
BseDI CCNNGG 1 cut(s) 180
BseGI GGATG 1 cut(s) 206
BseLI CCNNNNNNNGG 2 cut(s) 375, 947
BseMI GCAATG 2 cut(s) 335, 501
BseMII CTCAG 3 cut(s) 611, 862, 969
BseNI ACTGG 1 cut(s) 962
BseRI GAGGAG 1 cut(s) 806
BseSI GKGCMC 1 cut(s) 701
BseX3I CGGCCG 1 cut(s) 159
BseXI GCAGC 2 cut(s) 497, 1022
BsgI GTGCAG 1 cut(s) 1029
Bsh1236I CGCG 2 cut(s) 91, 307
Bsh1285I CGRYCG 1 cut(s) 162
BshFI GGCC 5 cut(s) 134, 161, 179, 459, 764
BsiEI CGRYCG 1 cut(s) 162
BsiHKAI GWGCWC 1 cut(s) 102
BsiSI CCGG 1 cut(s) 199
BslFI GGGAC 1 cut(s) 968
BslI CCNNNNNNNGG 2 cut(s) 375, 947
BsmAI GTCTC 1 cut(s) 291
BsmFI GGGAC 1 cut(s) 968
BsnI GGCC 5 cut(s) 134, 161, 179, 459, 764
Bsp1286I GDGCHC 3 cut(s) 102, 701, 942
Bsp1407I TGTACA 1 cut(s) 442
Bsp143I GATC 9 cut(s) 63, 82, 139, 185, 201, 229, 523, 534, 779
BspACI CCGC 4 cut(s) 112, 301, 483, 601
BspANI GGCC 5 cut(s) 134, 161, 179, 459, 764
BspCNI CTCAG 3 cut(s) 612, 861, 970
BspFNI CGCG 2 cut(s) 91, 307
BspHI TCATGA 1 cut(s) 690
BspLI GGNNCC 1 cut(s) 153
BspPI GGATC 6 cut(s) 58, 77, 193, 209, 224, 787
BsrDI GCAATG 2 cut(s) 335, 501
BsrGI TGTACA 1 cut(s) 442
BsrI ACTGG 1 cut(s) 962
BssECI CCNNGG 1 cut(s) 180
BssMI GATC 9 cut(s) 63, 82, 139, 185, 201, 229, 523, 534, 779
Bst2UI CCWGG 1 cut(s) 1046
Bst4CI ACNGT 2 cut(s) 347, 577
Bst6I CTCTTC 3 cut(s) 217, 462, 774
BstAPI GCANNNNNTGC 1 cut(s) 730
BstAUI TGTACA 1 cut(s) 442
BstC8I GCNNGC 5 cut(s) 305, 311, 701, 762, 861
BstDEI CTNAG 3 cut(s) 620, 848, 978
BstDSI CCRYGG 1 cut(s) 180
BstENI CCTNNNNNAGG 1 cut(s) 373
BstF5I GGATG 1 cut(s) 206
BstFNI CGCG 2 cut(s) 91, 307
BstHHI GCGC 4 cut(s) 75, 93, 309, 1015
BstKTI GATC 9 cut(s) 66, 85, 142, 188, 204, 232, 526, 537, 782
BstMAI GTCTC 1 cut(s) 291
BstMBI GATC 9 cut(s) 63, 82, 139, 185, 201, 229, 523, 534, 779
BstMCI CGRYCG 1 cut(s) 162
BstMWI GCNNNNNNNGC 5 cut(s) 97, 158, 491, 730, 983
BstNI CCWGG 1 cut(s) 1046
BstNSI RCATGY 2 cut(s) 313, 724
BstSCI CCNGG 1 cut(s) 1044
BstSFI CTRYAG 1 cut(s) 552
BstSLI GKGCMC 1 cut(s) 701
BstUI CGCG 2 cut(s) 91, 307
BstV1I GCAGC 2 cut(s) 497, 1022
BstV2I GAAGAC 1 cut(s) 747
BstX2I RGATCY 1 cut(s) 201
BstYI RGATCY 1 cut(s) 201
BstZI CGGCCG 1 cut(s) 159
BsuI GTATCC 1 cut(s) 285
BsuRI GGCC 5 cut(s) 134, 161, 179, 459, 764
BtgI CCRYGG 1 cut(s) 180
BtrI CACGTC 1 cut(s) 330
BtsCI GGATG 1 cut(s) 206
BtsI GCAGTG 1 cut(s) 64
BtsIMutI CAGTG 2 cut(s) 64, 352
Cac8I GCNNGC 5 cut(s) 305, 311, 701, 762, 861
CciI TCATGA 1 cut(s) 690
CfoI GCGC 4 cut(s) 75, 93, 309, 1015
Cfr13I GGNCC 2 cut(s) 133, 628
CseI GACGC 1 cut(s) 97
Csp6I GTAC 1 cut(s) 443
CspCI CAANNNNNGTGG 2 cut(s) 233, 268
CviAII CATG 8 cut(s) 137, 310, 314, 512, 625, 691, 721, 970
CviQI GTAC 1 cut(s) 443
DdeI CTNAG 3 cut(s) 620, 848, 978
DpnI GATC 9 cut(s) 65, 84, 141, 187, 203, 231, 525, 536, 781
DpnII GATC 9 cut(s) 63, 82, 139, 185, 201, 229, 523, 534, 779
EaeI YGGCCR 2 cut(s) 159, 457
EagI CGGCCG 1 cut(s) 159
Eam1104I CTCTTC 3 cut(s) 217, 462, 774
EarI CTCTTC 3 cut(s) 217, 462, 774
EclXI CGGCCG 1 cut(s) 159
Eco24I GRGCYC 1 cut(s) 942
Eco47I GGWCC 1 cut(s) 628
Eco52I CGGCCG 1 cut(s) 159
EcoNI CCTNNNNNAGG 1 cut(s) 373
EcoRI GAATTC 1 cut(s) 22
EcoRII CCWGG 1 cut(s) 1044
EcoT22I ATGCAT 2 cut(s) 315, 887
EcoT38I GRGCYC 1 cut(s) 942
FaeI CATG 8 cut(s) 140, 313, 317, 515, 628, 694, 724, 973
FaqI GGGAC 1 cut(s) 968
FatI CATG 8 cut(s) 136, 309, 313, 511, 624, 690, 720, 969
FbaI TGATCA 2 cut(s) 139, 523
Fnu4HI GCNGC 3 cut(s) 483, 486, 1011
FokI GGATG 1 cut(s) 193
FriOI GRGCYC 1 cut(s) 942
Fsp4HI GCNGC 3 cut(s) 483, 486, 1011
FspBI CTAG 3 cut(s) 104, 290, 947
GlaI GCGC 4 cut(s) 74, 92, 308, 1014
GluI GCNGC 3 cut(s) 483, 486, 1011
GsuI CTGGAG 1 cut(s) 725
HaeIII GGCC 5 cut(s) 134, 161, 179, 459, 764
HapII CCGG 1 cut(s) 199
HgaI GACGC 1 cut(s) 97
HhaI GCGC 4 cut(s) 75, 93, 309, 1015
Hin1II CATG 8 cut(s) 140, 313, 317, 515, 628, 694, 724, 973
Hin6I GCGC 4 cut(s) 73, 91, 307, 1013
HinP1I GCGC 4 cut(s) 73, 91, 307, 1013
HindIII AAGCTT 1 cut(s) 583
HinfI GANTC 1 cut(s) 381
HpaII CCGG 1 cut(s) 199
HphI GGTGA 2 cut(s) 70, 590
Hpy166II GTNNAC 1 cut(s) 395
Hpy188I TCNGA 7 cut(s) 21, 87, 144, 217, 234, 621, 964
Hpy188III TCNNGA 3 cut(s) 67, 290, 691
Hpy8I GTNNAC 1 cut(s) 395
Hpy99I CGWCG 3 cut(s) 44, 91, 458
HpyAV CCTTC 3 cut(s) 139, 169, 945
HpyCH4III ACNGT 2 cut(s) 347, 577
HpyCH4IV ACGT 1 cut(s) 329
HpyCH4V TGCA 7 cut(s) 313, 416, 635, 724, 863, 885, 1010
HpyF10VI GCNNNNNNNGC 5 cut(s) 97, 158, 491, 730, 983
HpyF3I CTNAG 3 cut(s) 620, 848, 978
HpySE526I ACGT 1 cut(s) 329
Hsp92II CATG 8 cut(s) 140, 313, 317, 515, 628, 694, 724, 973
HspAI GCGC 4 cut(s) 73, 91, 307, 1013
Ksp22I TGATCA 2 cut(s) 139, 523
Kzo9I GATC 9 cut(s) 63, 82, 139, 185, 201, 229, 523, 534, 779
LmnI GCTCC 6 cut(s) 22, 97, 157, 544, 616, 874
Lsp1109I GCAGC 2 cut(s) 497, 1022
LweI GCATC 4 cut(s) 60, 60, 403, 469
MaeI CTAG 3 cut(s) 104, 290, 947
MaeII ACGT 1 cut(s) 329
MalI GATC 9 cut(s) 65, 84, 141, 187, 203, 231, 525, 536, 781
MboI GATC 9 cut(s) 63, 82, 139, 185, 201, 229, 523, 534, 779
MboII GAAGA 7 cut(s) 204, 479, 752, 761, 764, 931, 1039
MflI RGATCY 1 cut(s) 201
MhlI GDGCHC 3 cut(s) 102, 701, 942
MluCI AATT 5 cut(s) 22, 279, 423, 665, 990
MmeI TCCRAC 5 cut(s) 51, 57, 110, 179, 396
Mph1103I ATGCAT 2 cut(s) 315, 887
MseI TTAA 6 cut(s) 816, 879, 929, 1032, 1055, 1060
MslI CAYNNNNRTG 1 cut(s) 308
MspA1I CMGCKG 1 cut(s) 249
MspI CCGG 1 cut(s) 199
MspR9I CCNGG 1 cut(s) 1046
MvaI CCWGG 1 cut(s) 1046
MvnI CGCG 2 cut(s) 91, 307
MwoI GCNNNNNNNGC 5 cut(s) 97, 158, 491, 730, 983
NdeII GATC 9 cut(s) 63, 82, 139, 185, 201, 229, 523, 534, 779
NlaIII CATG 8 cut(s) 140, 313, 317, 515, 628, 694, 724, 973
NlaIV GGNNCC 1 cut(s) 153
NsiI ATGCAT 2 cut(s) 315, 887
NspI RCATGY 2 cut(s) 313, 724
PaeI GCATGC 1 cut(s) 313
PagI TCATGA 1 cut(s) 690
PfeI GAWTC 1 cut(s) 381
PkrI GCNGC 3 cut(s) 484, 487, 1012
Psp6I CCWGG 1 cut(s) 1044
PspGI CCWGG 1 cut(s) 1044
PspN4I GGNNCC 1 cut(s) 153
PspPI GGNCC 2 cut(s) 133, 628
PsuI RGATCY 1 cut(s) 201
PvuII CAGCTG 1 cut(s) 249
RsaI GTAC 1 cut(s) 444
RsaNI GTAC 1 cut(s) 443
RseI CAYNNNNRTG 1 cut(s) 308
SaqAI TTAA 6 cut(s) 816, 879, 929, 1032, 1055, 1060
SatI GCNGC 3 cut(s) 483, 486, 1011
Sau3AI GATC 9 cut(s) 63, 82, 139, 185, 201, 229, 523, 534, 779
Sau96I GGNCC 2 cut(s) 133, 628
ScrFI CCNGG 1 cut(s) 1046
SduI GDGCHC 3 cut(s) 102, 701, 942
SfaNI GCATC 4 cut(s) 60, 60, 403, 469
SfcI CTRYAG 1 cut(s) 552
SinI GGWCC 1 cut(s) 628
SmiMI CAYNNNNRTG 1 cut(s) 308
SmlI CTYRAG 2 cut(s) 46, 837
SmoI CTYRAG 2 cut(s) 46, 837
SphI GCATGC 1 cut(s) 313
Sse9I AATT 5 cut(s) 22, 279, 423, 665, 990
SsiI CCGC 4 cut(s) 112, 301, 483, 601
SspI AATATT 2 cut(s) 820, 934
SspMI CTAG 3 cut(s) 104, 290, 947
StyD4I CCNGG 1 cut(s) 1044
TaaI ACNGT 2 cut(s) 347, 577
TaiI ACGT 1 cut(s) 332
TaqI TCGA 6 cut(s) 257, 411, 537, 558, 789, 910
TasI AATT 5 cut(s) 22, 279, 423, 665, 990
TatI WGTACW 1 cut(s) 442
TauI GCSGC 1 cut(s) 485
TfiI GAWTC 1 cut(s) 381
Tru1I TTAA 6 cut(s) 816, 879, 929, 1032, 1055, 1060
Tru9I TTAA 6 cut(s) 816, 879, 929, 1032, 1055, 1060
TscAI CASTG 2 cut(s) 64, 352
TseI GCWGC 2 cut(s) 485, 1010
TspDTI ATGAA 5 cut(s) 500, 508, 512, 679, 753
TspGWI ACGGA 1 cut(s) 931
TspRI CASTG 2 cut(s) 64, 352
VpaK11BI GGWCC 1 cut(s) 628
XagI CCTNNNNNAGG 1 cut(s) 373
XapI RAATTY 2 cut(s) 22, 423
XbaI TCTAGA 1 cut(s) 289
XceI RCATGY 2 cut(s) 313, 724
XspI CTAG 3 cut(s) 104, 290, 947
Zsp2I ATGCAT 2 cut(s) 315, 887
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.