Rw2G007220

RRP12-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000835
Physical Location & Seq
Forward (+)
6996499 .. 6999315
2817 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G007220.1

Sequence Viewer

Length: 1437 bp
ATGGAAACCCTAACCGCCGTAGACGACGGAAACATAGGCACCTCAATCCTGATGCGCTGCACCGACCCCAACAACCAAGTCGACCTCGAAATCCGCAAACTCATCGTCGTCATGGCCCAGCTCTTCCGAGACCACAACGCTGCTCGGACGCCGGAAGCCTACCTGGGCTCCACCTGCTCCTCTCTCGACCTCGTCGCCTCCACCTTCGACGTTTCCAACCCCAAACGTCCCACTGACGTCATCGACGCCCACCTCACAGTTCTCTCCATCGTCGTTCCGAAAGCCCCCGCCAGATTTCTGATGAGCAATCTCCAGTTGATCGTTGCTTGTTTGAGGCGACCTCTCCGGTCTAAGTTTCCGTTTCCGTTGGACGCCACTACCTGCTGCTTAGGAGTTAAATGCCTTGCTCACTTGATCATCGTTATCACAAAACGCTTCAACAGCTATATCTGCAAGGAGAGTCAAGTAGTTCAGCGCAAAGTTTTTAAGGCCATGCTTGCTTTGGATGCTGTGGCAGTACTTCTTCCCGTTATGTCAACCCAGGACAGAACTGCTGTGCTCTTCTACCATAAAACTCTGTTTGAACTGCAACAAGGCTGTGTTACATTTCGCATAATAAATGGTGTGCATAGAGTTTGTCTCAACCCATCTCCAGACGTTTCTCCTGAATTGTTGCTCGATCTAATATGCTCGATATGTCATTCTGTCTCCAAACATGAGACACATTATATTGTGAAAAATATATCTGAATTGCTGGGTACTGGAGTGGCGAAAGTCTATTCCTTGAACATTAAAATCTGTGAAACTAAGCTCCCTATTGTGTTTGATGCACTCAAAGTTATAATGGTAAAGCCTGAAGAAGAAGACATCGCTCATGCAGCACAGGCATTTAGGTGTTTAATTCGTGCTTGCATTGATGAAAGCTTGATCAAACAGGGAGTTGACCGTATTCTGATGAATGCAAATATGTATAACAGAACAAACTCTGTGCCAACTATAATTGAAAAATTGTGTGCTACTATTCAAAGCTTATTTGGTTATTCCGACGCTACTATCCTGTCCCTTGCTTTTGAAATTGTCTCAACAATGTTCGAGAAACTAGGGGTATACTCTTCTTATTTGATGAAGGGTACACTGAAGACCTTAGCAGACATGCAGAAGTTGCCTGATCAAGAGTTCCGTTTCAAGGATGAGGTCAAGCTTGTTCTACAAGTGGTTGTTGAGAAATGGCGGCGTGATGCTATAATGGCTGTCTTGCCTCAAGAACACATGGAACTGCTTGCAGAGATCAATGAACAGCTGGGTTCTAAATCTGTGGAGGTGAGATCTAATGTGTCCACAGCAACTACTGTCAGAAACAATCAAGCGTATGATAAAGCACCATGTAACAATTCAAAATGGGAGATGGATGATCCAGATATTGAGTATGAGGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NUC173

Protein Analysis

478

Amino Acids

53.53

Weight (kDa)

6.57

Isoelectric Point (pI)

43.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HEAT_RRP12_N PF25772 6 - 144 5.4e-19 RRP12-like, N-terminal HEAT repeats
HEAT_RRP12_N PF25772 155 - 219 3.7e-07 RRP12-like, N-terminal HEAT repeats
RRP12_HEAT PF08161 289 - 422 4.7e-15 Ribosomal RNA-processing protein 12, HEAT repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 842
AarI CACCTGC 1 cut(s) 182
AatII GACGTC 1 cut(s) 240
Acc36I ACCTGC 2 cut(s) 182, 389
AccB1I GGYRCC 1 cut(s) 38
AccI GTMKAC 3 cut(s) 21, 81, 1107
AciI CCGC 4 cut(s) 15, 94, 288, 1231
AclWI GGATC 1 cut(s) 1406
AcuI CTGAAG 2 cut(s) 876, 1157
AcyI GRCGYC 4 cut(s) 149, 237, 246, 372
AfaI GTAC 3 cut(s) 519, 760, 1132
AfiI CCNNNNNNNGG 1 cut(s) 1186
AgsI TTSAA 8 cut(s) 439, 584, 787, 1004, 1025, 1073, 1186, 1395
AjnI CCWGG 2 cut(s) 162, 540
AluBI AGCT 7 cut(s) 121, 444, 811, 924, 1029, 1201, 1300
AluI AGCT 7 cut(s) 121, 444, 811, 924, 1029, 1201, 1300
Alw21I GWGCWC 1 cut(s) 561
Alw26I GTCTC 5 cut(s) 123, 644, 712, 713, 1084
AlwI GGATC 1 cut(s) 1406
AoxI GGCC 3 cut(s) 114, 489, 1431
ApeKI GCWGC 4 cut(s) 57, 140, 384, 878
ArsI GACNNNNNNTTYG 2 cut(s) 90, 122
AspLEI GCGC 2 cut(s) 57, 477
AspS9I GGNCC 1 cut(s) 115
AsuHPI GGTGA 1 cut(s) 1333
BaeI ACNNNNGTAYC 2 cut(s) 750, 783
BanI GGYRCC 1 cut(s) 38
BanII GRGCYC 1 cut(s) 170
BbsI GAAGAC 2 cut(s) 870, 1145
Bbv12I GWGCWC 1 cut(s) 561
BbvI GCAGC 4 cut(s) 44, 127, 371, 890
BccI CCATC 3 cut(s) 275, 655, 1399
BceAI ACGGC 1 cut(s) 2
BcgI CGANNNNNNTGC 2 cut(s) 85, 119
BciT130I CCWGG 2 cut(s) 164, 542
BclI TGATCA 3 cut(s) 414, 927, 1168
BcoDI GTCTC 5 cut(s) 123, 644, 712, 713, 1084
BfaI CTAG 1 cut(s) 1100
BfuAI ACCTGC 2 cut(s) 182, 389
BglII AGATCT 1 cut(s) 1325
BisI GCNGC 5 cut(s) 58, 141, 385, 879, 1232
BlsI GCNGC 5 cut(s) 59, 142, 386, 880, 1233
BmcAI AGTACT 1 cut(s) 519
Bme1390I CCNGG 2 cut(s) 164, 542
BmgT120I GGNCC 1 cut(s) 115
BmiI GGNNCC 2 cut(s) 40, 169
BmrFI CCNGG 2 cut(s) 164, 542
BmsI GCATC 4 cut(s) 42, 496, 817, 1228
BpiI GAAGAC 2 cut(s) 870, 1145
BplI GAGNNNNNCTC 4 cut(s) 325, 357, 624, 656
BpmI CTGGAG 3 cut(s) 296, 636, 783
Bpu10I CCTNAGC 2 cut(s) 388, 1144
BpuEI CTTGAG 1 cut(s) 1245
BsaHI GRCGYC 4 cut(s) 149, 237, 246, 372
BsaI GGTCTC 1 cut(s) 123
BsaJI CCNNGG 2 cut(s) 163, 540
BsaWI WCCGGW 1 cut(s) 345
BsaXI ACNNNNNCTCC 2 cut(s) 152, 182
Bsc4I CCNNNNNNNGG 1 cut(s) 1186
Bse1I ACTGG 2 cut(s) 313, 766
BseBI CCWGG 2 cut(s) 164, 542
BseDI CCNNGG 2 cut(s) 163, 540
BseGI GGATG 3 cut(s) 511, 1195, 1414
BseLI CCNNNNNNNGG 1 cut(s) 1186
BseNI ACTGG 2 cut(s) 313, 766
BseRI GAGGAG 1 cut(s) 169
BseXI GCAGC 4 cut(s) 44, 127, 371, 890
BseYI CCCAGC 3 cut(s) 117, 754, 1300
BsgI GTGCAG 1 cut(s) 43
BshFI GGCC 3 cut(s) 116, 491, 1433
BshNI GGYRCC 1 cut(s) 38
BsiHKAI GWGCWC 1 cut(s) 561
BsiSI CCGG 2 cut(s) 152, 346
BslFI GGGAC 2 cut(s) 213, 1045
BslI CCNNNNNNNGG 1 cut(s) 1186
BsmAI GTCTC 5 cut(s) 123, 644, 712, 713, 1084
BsmFI GGGAC 2 cut(s) 213, 1045
BsmI GAATGC 1 cut(s) 964
BsnI GGCC 3 cut(s) 116, 491, 1433
Bso31I GGTCTC 1 cut(s) 123
Bsp1286I GDGCHC 2 cut(s) 170, 561
Bsp143I GATC 8 cut(s) 318, 414, 679, 927, 1168, 1287, 1325, 1411
BspACI CCGC 4 cut(s) 15, 94, 288, 1231
BspANI GGCC 3 cut(s) 116, 491, 1433
BspLI GGNNCC 2 cut(s) 40, 169
BspMI ACCTGC 2 cut(s) 182, 389
BspPI GGATC 1 cut(s) 1406
BspQI GCTCTTC 2 cut(s) 128, 566
BspT107I GGYRCC 1 cut(s) 38
BspTNI GGTCTC 1 cut(s) 123
BsrI ACTGG 2 cut(s) 313, 766
BssECI CCNNGG 2 cut(s) 163, 540
BssMI GATC 8 cut(s) 318, 414, 679, 927, 1168, 1287, 1325, 1411
BssNAI GTATAC 1 cut(s) 1108
BssNI GRCGYC 4 cut(s) 149, 237, 246, 372
Bst1107I GTATAC 1 cut(s) 1108
Bst2UI CCWGG 2 cut(s) 164, 542
Bst4CI ACNGT 3 cut(s) 259, 947, 1351
Bst6I CTCTTC 3 cut(s) 128, 566, 1117
BstACI GRCGYC 4 cut(s) 149, 237, 246, 372
BstAPI GCANNNNNTGC 1 cut(s) 1162
BstC8I GCNNGC 3 cut(s) 498, 910, 1281
BstDEI CTNAG 4 cut(s) 351, 388, 807, 1144
BstF5I GGATG 3 cut(s) 511, 1195, 1414
BstHHI GCGC 2 cut(s) 57, 477
BstKTI GATC 8 cut(s) 321, 417, 682, 930, 1171, 1290, 1328, 1414
BstMAI GTCTC 5 cut(s) 123, 644, 712, 713, 1084
BstMBI GATC 8 cut(s) 318, 414, 679, 927, 1168, 1287, 1325, 1411
BstMWI GCNNNNNNNGC 9 cut(s) 174, 441, 450, 497, 506, 878, 884, 1162, 1247
BstNI CCWGG 2 cut(s) 164, 542
BstNSI RCATGY 1 cut(s) 1156
BstSCI CCNGG 2 cut(s) 162, 540
BstV1I GCAGC 4 cut(s) 44, 127, 371, 890
BstV2I GAAGAC 2 cut(s) 870, 1145
BstX2I RGATCY 1 cut(s) 1325
BstYI RGATCY 1 cut(s) 1325
BstZ17I GTATAC 1 cut(s) 1108
BsuRI GGCC 3 cut(s) 116, 491, 1433
BtgZI GCGATG 1 cut(s) 853
BtsCI GGATG 3 cut(s) 511, 1195, 1414
BtsIMutI CAGTG 2 cut(s) 231, 1133
BveI ACCTGC 2 cut(s) 182, 389
Cac8I GCNNGC 3 cut(s) 498, 910, 1281
CfoI GCGC 2 cut(s) 57, 477
Cfr13I GGNCC 1 cut(s) 115
CseI GACGC 4 cut(s) 157, 254, 380, 1055
Csp6I GTAC 3 cut(s) 518, 759, 1131
CviAII CATG 7 cut(s) 112, 493, 716, 875, 1153, 1270, 1383
CviQI GTAC 3 cut(s) 518, 759, 1131
DdeI CTNAG 4 cut(s) 351, 388, 807, 1144
DpnI GATC 8 cut(s) 320, 416, 681, 929, 1170, 1289, 1327, 1413
DpnII GATC 8 cut(s) 318, 414, 679, 927, 1168, 1287, 1325, 1411
Eam1104I CTCTTC 3 cut(s) 128, 566, 1117
EarI CTCTTC 3 cut(s) 128, 566, 1117
Eco147I AGGCCT 1 cut(s) 1433
Eco24I GRGCYC 1 cut(s) 170
Eco31I GGTCTC 1 cut(s) 123
Eco57I CTGAAG 2 cut(s) 876, 1157
EcoRII CCWGG 2 cut(s) 162, 540
EcoT38I GRGCYC 1 cut(s) 170
FaeI CATG 7 cut(s) 115, 496, 719, 878, 1156, 1273, 1386
FaqI GGGAC 2 cut(s) 213, 1045
FatI CATG 7 cut(s) 111, 492, 715, 874, 1152, 1269, 1382
FauI CCCGC 1 cut(s) 295
FbaI TGATCA 3 cut(s) 414, 927, 1168
FblI GTMKAC 3 cut(s) 21, 81, 1107
Fnu4HI GCNGC 5 cut(s) 58, 141, 385, 879, 1232
FokI GGATG 3 cut(s) 518, 1202, 1421
FriOI GRGCYC 1 cut(s) 170
Fsp4HI GCNGC 5 cut(s) 58, 141, 385, 879, 1232
FspBI CTAG 1 cut(s) 1100
GlaI GCGC 2 cut(s) 56, 476
GluI GCNGC 5 cut(s) 58, 141, 385, 879, 1232
GsaI CCCAGC 3 cut(s) 121, 758, 1304
GsuI CTGGAG 3 cut(s) 296, 636, 783
HaeIII GGCC 3 cut(s) 116, 491, 1433
HapII CCGG 2 cut(s) 152, 346
HgaI GACGC 4 cut(s) 157, 254, 380, 1055
HhaI GCGC 2 cut(s) 57, 477
Hin1I GRCGYC 4 cut(s) 149, 237, 246, 372
Hin1II CATG 7 cut(s) 115, 496, 719, 878, 1156, 1273, 1386
Hin6I GCGC 2 cut(s) 55, 475
HinP1I GCGC 2 cut(s) 55, 475
HincII GTYRAC 3 cut(s) 82, 537, 943
HindII GTYRAC 3 cut(s) 82, 537, 943
HindIII AAGCTT 3 cut(s) 922, 1027, 1199
HinfI GANTC 1 cut(s) 460
HpaII CCGG 2 cut(s) 152, 346
HphI GGTGA 1 cut(s) 1333
Hpy166II GTNNAC 7 cut(s) 22, 82, 537, 943, 1108, 1133, 1338
Hpy188I TCNGA 8 cut(s) 128, 147, 279, 300, 748, 954, 1045, 1355
Hpy188III TCNNGA 8 cut(s) 49, 185, 653, 665, 1093, 1172, 1262, 1415
Hpy8I GTNNAC 7 cut(s) 22, 82, 537, 943, 1108, 1133, 1338
Hpy99I CGWCG 7 cut(s) 29, 110, 197, 212, 248, 275, 1049
HpyAV CCTTC 2 cut(s) 214, 1120
HpyCH4III ACNGT 3 cut(s) 259, 947, 1351
HpyCH4IV ACGT 4 cut(s) 210, 226, 237, 657
HpyF10VI GCNNNNNNNGC 9 cut(s) 174, 441, 450, 497, 506, 878, 884, 1162, 1247
HpyF3I CTNAG 4 cut(s) 351, 388, 807, 1144
HpySE526I ACGT 4 cut(s) 210, 226, 237, 657
Hsp92I GRCGYC 4 cut(s) 149, 237, 246, 372
Hsp92II CATG 7 cut(s) 115, 496, 719, 878, 1156, 1273, 1386
HspAI GCGC 2 cut(s) 55, 475
Ksp22I TGATCA 3 cut(s) 414, 927, 1168
Kzo9I GATC 8 cut(s) 318, 414, 679, 927, 1168, 1287, 1325, 1411
LguI GCTCTTC 2 cut(s) 128, 566
LmnI GCTCC 3 cut(s) 173, 182, 816
Lsp1109I GCAGC 4 cut(s) 44, 127, 371, 890
LweI GCATC 4 cut(s) 42, 496, 817, 1228
MaeI CTAG 1 cut(s) 1100
MaeII ACGT 4 cut(s) 210, 226, 237, 657
MaeIII GTNAC 2 cut(s) 601, 1385
MalI GATC 8 cut(s) 320, 416, 681, 929, 1170, 1289, 1327, 1413
MboI GATC 8 cut(s) 318, 414, 679, 927, 1168, 1287, 1325, 1411
MboII GAAGA 8 cut(s) 115, 515, 553, 869, 872, 875, 1104, 1150
MflI RGATCY 1 cut(s) 1325
MhlI GDGCHC 2 cut(s) 170, 561
MluCI AATT 7 cut(s) 668, 749, 900, 999, 1007, 1074, 1390
MlyI GAGTC 1 cut(s) 469
MmeI TCCRAC 3 cut(s) 240, 348, 1068
MseI TTAA 4 cut(s) 396, 486, 792, 899
MslI CAYNNNNRTG 1 cut(s) 892
MspA1I CMGCKG 1 cut(s) 1300
MspI CCGG 2 cut(s) 152, 346
MspR9I CCNGG 2 cut(s) 164, 542
Mva1269I GAATGC 1 cut(s) 964
MvaI CCWGG 2 cut(s) 164, 542
MwoI GCNNNNNNNGC 9 cut(s) 174, 441, 450, 497, 506, 878, 884, 1162, 1247
NdeII GATC 8 cut(s) 318, 414, 679, 927, 1168, 1287, 1325, 1411
NlaIII CATG 7 cut(s) 115, 496, 719, 878, 1156, 1273, 1386
NlaIV GGNNCC 2 cut(s) 40, 169
NspI RCATGY 1 cut(s) 1156
PaqCI CACCTGC 1 cut(s) 182
PceI AGGCCT 1 cut(s) 1433
PciSI GCTCTTC 2 cut(s) 128, 566
PctI GAATGC 1 cut(s) 964
PflFI GACNNNGTC 1 cut(s) 191
PkrI GCNGC 5 cut(s) 59, 142, 386, 880, 1233
PleI GAGTC 1 cut(s) 468
PpsI GAGTC 1 cut(s) 468
PsiI TTATAA 1 cut(s) 842
Psp6I CCWGG 2 cut(s) 162, 540
PspFI CCCAGC 3 cut(s) 117, 754, 1300
PspGI CCWGG 2 cut(s) 162, 540
PspN4I GGNNCC 2 cut(s) 40, 169
PspPI GGNCC 1 cut(s) 115
PsuI RGATCY 1 cut(s) 1325
PsyI GACNNNGTC 1 cut(s) 191
PvuII CAGCTG 1 cut(s) 1300
RsaI GTAC 3 cut(s) 519, 760, 1132
RsaNI GTAC 3 cut(s) 518, 759, 1131
RseI CAYNNNNRTG 1 cut(s) 892
SalI GTCGAC 1 cut(s) 80
SapI GCTCTTC 2 cut(s) 128, 566
SaqAI TTAA 4 cut(s) 396, 486, 792, 899
SatI GCNGC 5 cut(s) 58, 141, 385, 879, 1232
Sau3AI GATC 8 cut(s) 318, 414, 679, 927, 1168, 1287, 1325, 1411
Sau96I GGNCC 1 cut(s) 115
ScaI AGTACT 1 cut(s) 519
SchI GAGTC 1 cut(s) 469
ScrFI CCNGG 2 cut(s) 164, 542
SduI GDGCHC 2 cut(s) 170, 561
SfaNI GCATC 4 cut(s) 42, 496, 817, 1228
SmiMI CAYNNNNRTG 1 cut(s) 892
SmlI CTYRAG 1 cut(s) 1260
SmoI CTYRAG 1 cut(s) 1260
Sse9I AATT 7 cut(s) 668, 749, 900, 999, 1007, 1074, 1390
SseBI AGGCCT 1 cut(s) 1433
SsiI CCGC 4 cut(s) 15, 94, 288, 1231
SspMI CTAG 1 cut(s) 1100
StuI AGGCCT 1 cut(s) 1433
StyD4I CCNGG 2 cut(s) 162, 540
TaaI ACNGT 3 cut(s) 259, 947, 1351
TaiI ACGT 4 cut(s) 213, 229, 240, 660
TaqI TCGA 8 cut(s) 81, 87, 186, 207, 243, 678, 692, 1092
TasI AATT 7 cut(s) 668, 749, 900, 999, 1007, 1074, 1390
TatI WGTACW 1 cut(s) 517
TauI GCSGC 1 cut(s) 1234
Tru1I TTAA 4 cut(s) 396, 486, 792, 899
Tru9I TTAA 4 cut(s) 396, 486, 792, 899
TscAI CASTG 2 cut(s) 238, 1140
TseI GCWGC 4 cut(s) 57, 140, 384, 878
TspDTI ATGAA 4 cut(s) 933, 971, 1139, 1308
TspGWI ACGGA 4 cut(s) 42, 348, 354, 1169
TspRI CASTG 2 cut(s) 238, 1140
Tth111I GACNNNGTC 1 cut(s) 191
XceI RCATGY 1 cut(s) 1156
XcmI CCANNNNNNNNNTGG 1 cut(s) 499
XmiI GTMKAC 3 cut(s) 21, 81, 1107
XspI CTAG 1 cut(s) 1100
ZraI GACGTC 1 cut(s) 238
ZrmI AGTACT 1 cut(s) 519
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.