Rroxscaffold_2G00146720

RRP12-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000835
Physical Location & Seq
Reverse (-)
84590512 .. 84594251
3740 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00146720.1

Sequence Viewer

Length: 705 bp
ATGTCAACCCAGGACAGGACTGCTGTAACAGGACAGGACTTCTACTATAAAACTCTGTTTGAACTGCAACAAGGCTATGTTACATTTCGCCTAATAAATGGTGTGCATAGAGTTTGTCTCAACCCATCTCCAGACGTTTCTCCTGAATTGTTGCTCGATCTAATATGCTCGATATGTCATTCTGTCTCCAAACATGAGACAGATTATATTGTGAAAAGTATATCTGAATTGCTGGGTACTGGAGTGGCGAAAGTCTATTCCTTGAACAGGAAAATCTGCGAATCTAAGCTCCCTATTGTGTTTGATGCACTCAAAGTTATAATGGTAAAGCCTAAAGAAGAAGACATCGCTCATGCAGCACAGGCATTTAAGTGTTTAATTCGTGCTTGCATCGATGAAAGCTTGATCAAACAGGGAGTTGACCGTATTCTGATGAATGCAAATGTGTATAACAGAACAAACTCTGAGCCAACTATAATTGAAAAATTGTGTGCTACTATTCAAAGCTTATTTGGTTATTCCGACACTGCTATCCTGTACCTTGCTTTTGAAATTGTCTCAACAATGTTCGAGAAACTAGGGGTATACTCTTCTTATTTGATGAAGGCTACACTGAAGACCTTAGCAGACATGCAGAAGTTGCCTGATCAAGAGTTCCGTTTCAAGGAGGAGGTCAAGCTTGTTCTACAAGAAATGGGGGCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NUC173

Protein Analysis

234

Amino Acids

26.43

Weight (kDa)

6.12

Isoelectric Point (pI)

32.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRP12_HEAT PF08161 113 - 230 2.5e-14 Ribosomal RNA-processing protein 12, HEAT repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 320
AccI GTMKAC 1 cut(s) 585
AcuI CTGAAG 1 cut(s) 635
AfaI GTAC 2 cut(s) 238, 539
AfiI CCNNNNNNNGG 3 cut(s) 15, 267, 664
AgsI TTSAA 6 cut(s) 62, 265, 482, 503, 551, 664
AjnI CCWGG 1 cut(s) 9
AluBI AGCT 4 cut(s) 289, 402, 507, 679
AluI AGCT 4 cut(s) 289, 402, 507, 679
Alw26I GTCTC 4 cut(s) 122, 190, 191, 562
ApeKI GCWGC 1 cut(s) 356
BaeI ACNNNNGTAYC 2 cut(s) 228, 261
BbsI GAAGAC 2 cut(s) 348, 623
BbvI GCAGC 1 cut(s) 368
BccI CCATC 1 cut(s) 133
BciT130I CCWGG 1 cut(s) 11
BclI TGATCA 2 cut(s) 405, 646
BcoDI GTCTC 4 cut(s) 122, 190, 191, 562
BfaI CTAG 1 cut(s) 578
BisI GCNGC 1 cut(s) 357
BlsI GCNGC 1 cut(s) 358
Bme1390I CCNGG 1 cut(s) 11
BmrFI CCNGG 1 cut(s) 11
BmsI GCATC 2 cut(s) 295, 399
BpiI GAAGAC 2 cut(s) 348, 623
BplI GAGNNNNNCTC 2 cut(s) 102, 134
BpmI CTGGAG 2 cut(s) 114, 261
Bpu10I CCTNAGC 1 cut(s) 622
Bsa29I ATCGAT 1 cut(s) 393
BsaJI CCNNGG 1 cut(s) 9
Bsc4I CCNNNNNNNGG 3 cut(s) 15, 267, 664
Bse1I ACTGG 1 cut(s) 244
BseBI CCWGG 1 cut(s) 11
BseCI ATCGAT 1 cut(s) 393
BseDI CCNNGG 1 cut(s) 9
BseLI CCNNNNNNNGG 3 cut(s) 15, 267, 664
BseMII CTCAG 1 cut(s) 456
BseNI ACTGG 1 cut(s) 244
BseRI GAGGAG 1 cut(s) 683
BseXI GCAGC 1 cut(s) 368
BseYI CCCAGC 1 cut(s) 232
BshVI ATCGAT 1 cut(s) 393
BslI CCNNNNNNNGG 3 cut(s) 15, 267, 664
BsmAI GTCTC 4 cut(s) 122, 190, 191, 562
BsmI GAATGC 1 cut(s) 442
Bsp143I GATC 3 cut(s) 157, 405, 646
BspCNI CTCAG 1 cut(s) 457
BspDI ATCGAT 1 cut(s) 393
BsrI ACTGG 1 cut(s) 244
BssECI CCNNGG 1 cut(s) 9
BssMI GATC 3 cut(s) 157, 405, 646
BssNAI GTATAC 1 cut(s) 586
Bst1107I GTATAC 1 cut(s) 586
Bst2UI CCWGG 1 cut(s) 11
Bst4CI ACNGT 1 cut(s) 425
Bst6I CTCTTC 1 cut(s) 595
BstAPI GCANNNNNTGC 1 cut(s) 640
BstC8I GCNNGC 1 cut(s) 388
BstDEI CTNAG 3 cut(s) 285, 465, 622
BstENI CCTNNNNNAGG 1 cut(s) 265
BstKTI GATC 3 cut(s) 160, 408, 649
BstMAI GTCTC 4 cut(s) 122, 190, 191, 562
BstMBI GATC 3 cut(s) 157, 405, 646
BstMWI GCNNNNNNNGC 3 cut(s) 356, 362, 640
BstNI CCWGG 1 cut(s) 11
BstNSI RCATGY 1 cut(s) 634
BstSCI CCNGG 1 cut(s) 9
BstV1I GCAGC 1 cut(s) 368
BstV2I GAAGAC 2 cut(s) 348, 623
BstZ17I GTATAC 1 cut(s) 586
Bsu15I ATCGAT 1 cut(s) 393
BsuTUI ATCGAT 1 cut(s) 393
BtgZI GCGATG 1 cut(s) 331
BtsI GCAGTG 1 cut(s) 525
BtsIMutI CAGTG 2 cut(s) 525, 611
Cac8I GCNNGC 1 cut(s) 388
ClaI ATCGAT 1 cut(s) 393
Csp6I GTAC 2 cut(s) 237, 538
CviAII CATG 3 cut(s) 194, 353, 631
CviJI RGCY 8 cut(s) 75, 289, 331, 402, 469, 507, 608, 679
CviKI_1 RGCY 8 cut(s) 75, 289, 331, 402, 469, 507, 608, 679
CviQI GTAC 2 cut(s) 237, 538
DdeI CTNAG 3 cut(s) 285, 465, 622
DpnI GATC 3 cut(s) 159, 407, 648
DpnII GATC 3 cut(s) 157, 405, 646
Eam1104I CTCTTC 1 cut(s) 595
EarI CTCTTC 1 cut(s) 595
Eco57I CTGAAG 1 cut(s) 635
EcoNI CCTNNNNNAGG 1 cut(s) 265
EcoRII CCWGG 1 cut(s) 9
FaeI CATG 3 cut(s) 197, 356, 634
FatI CATG 3 cut(s) 193, 352, 630
FbaI TGATCA 2 cut(s) 405, 646
FblI GTMKAC 1 cut(s) 585
Fnu4HI GCNGC 1 cut(s) 357
Fsp4HI GCNGC 1 cut(s) 357
FspBI CTAG 1 cut(s) 578
GluI GCNGC 1 cut(s) 357
GsaI CCCAGC 1 cut(s) 236
GsuI CTGGAG 2 cut(s) 114, 261
Hin1II CATG 3 cut(s) 197, 356, 634
HincII GTYRAC 2 cut(s) 6, 421
HindII GTYRAC 2 cut(s) 6, 421
HindIII AAGCTT 3 cut(s) 400, 505, 677
HinfI GANTC 1 cut(s) 281
Hpy166II GTNNAC 3 cut(s) 6, 421, 586
Hpy188I TCNGA 4 cut(s) 226, 432, 466, 523
Hpy188III TCNNGA 4 cut(s) 131, 143, 571, 650
Hpy8I GTNNAC 3 cut(s) 6, 421, 586
HpyAV CCTTC 1 cut(s) 598
HpyCH4III ACNGT 1 cut(s) 425
HpyCH4IV ACGT 1 cut(s) 135
HpyCH4V TGCA 7 cut(s) 67, 106, 308, 356, 390, 440, 634
HpyF10VI GCNNNNNNNGC 3 cut(s) 356, 362, 640
HpyF3I CTNAG 3 cut(s) 285, 465, 622
HpySE526I ACGT 1 cut(s) 135
Hsp92II CATG 3 cut(s) 197, 356, 634
Ksp22I TGATCA 2 cut(s) 405, 646
Kzo9I GATC 3 cut(s) 157, 405, 646
LmnI GCTCC 1 cut(s) 294
Lsp1109I GCAGC 1 cut(s) 368
LweI GCATC 2 cut(s) 295, 399
MaeI CTAG 1 cut(s) 578
MaeII ACGT 1 cut(s) 135
MaeIII GTNAC 2 cut(s) 25, 79
MalI GATC 3 cut(s) 159, 407, 648
MboI GATC 3 cut(s) 157, 405, 646
MboII GAAGA 4 cut(s) 350, 353, 582, 628
MluCI AATT 6 cut(s) 146, 227, 378, 477, 485, 552
MmeI TCCRAC 1 cut(s) 546
MnlI CCTC 2 cut(s) 661, 664
MseI TTAA 2 cut(s) 369, 377
MslI CAYNNNNRTG 1 cut(s) 370
MspR9I CCNGG 1 cut(s) 11
Mva1269I GAATGC 1 cut(s) 442
MvaI CCWGG 1 cut(s) 11
MwoI GCNNNNNNNGC 3 cut(s) 356, 362, 640
NdeII GATC 3 cut(s) 157, 405, 646
NlaIII CATG 3 cut(s) 197, 356, 634
NspI RCATGY 1 cut(s) 634
PctI GAATGC 1 cut(s) 442
PfeI GAWTC 1 cut(s) 281
PkrI GCNGC 1 cut(s) 358
PsiI TTATAA 1 cut(s) 320
Psp6I CCWGG 1 cut(s) 9
PspFI CCCAGC 1 cut(s) 232
PspGI CCWGG 1 cut(s) 9
RsaI GTAC 2 cut(s) 238, 539
RsaNI GTAC 2 cut(s) 237, 538
RseI CAYNNNNRTG 1 cut(s) 370
SaqAI TTAA 2 cut(s) 369, 377
SatI GCNGC 1 cut(s) 357
Sau3AI GATC 3 cut(s) 157, 405, 646
ScrFI CCNGG 1 cut(s) 11
SetI ASST 8 cut(s) 138, 291, 404, 509, 543, 623, 675, 681
SfaNI GCATC 2 cut(s) 295, 399
SmiMI CAYNNNNRTG 1 cut(s) 370
Sse9I AATT 6 cut(s) 146, 227, 378, 477, 485, 552
SspMI CTAG 1 cut(s) 578
StyD4I CCNGG 1 cut(s) 9
TaaI ACNGT 1 cut(s) 425
TaiI ACGT 1 cut(s) 138
TaqI TCGA 4 cut(s) 156, 170, 393, 570
TasI AATT 6 cut(s) 146, 227, 378, 477, 485, 552
TfiI GAWTC 1 cut(s) 281
Tru1I TTAA 2 cut(s) 369, 377
Tru9I TTAA 2 cut(s) 369, 377
TscAI CASTG 2 cut(s) 532, 618
TseI GCWGC 1 cut(s) 356
TspDTI ATGAA 3 cut(s) 411, 449, 617
TspGWI ACGGA 1 cut(s) 647
TspRI CASTG 2 cut(s) 532, 618
XagI CCTNNNNNAGG 1 cut(s) 265
XceI RCATGY 1 cut(s) 634
XmiI GTMKAC 1 cut(s) 585
XspI CTAG 1 cut(s) 578
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.