Rroxscaffold_2G00136050

RRP12-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000835
Physical Location & Seq
Forward (+)
73608919 .. 73613631
4713 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00136050.1

Sequence Viewer

Length: 675 bp
ATGGCGAGTGAATTGGCGGGATTGGAGCCGAAAGGCGAAATGAATACTAAGCTCGGCTATGCAACGTCTTTGTTGCTCAATAGTGGAGTGGCAAAAGTTTATTCATCCAACAGGGAATACTGTTTGAGTAAACTCCCTCTTGTGTTTGATGCACTCAAAATTTTAACGGGGAAACCTGTACAAGAGAATGAGGGTTGTATAGAAGAGGATATTGACGCCGCAGCACCTGCATTCAAGTGTCTTATTCATGCTTGCATCGATCAAGGCTTGATCAAACGGGAGCCGACTATAGTTGAAAAGCTGTGTTCTATTGTTCAAAGTTTACTTGGTTATCACCGCACAAATGCTCCTGCTCTCGGCATGGACCTTGCATTTGAGGTTGTTTCAACAATGTTCGATAAATTAGGGGCATACGCTTCTTACTTCATGAGGGGCACGCTGGAGAACTTGGCGGACATGCACAAGTTGCTTGATGAAGACTTCCCGTTTAGGAAGCAGGCCACTCTTCTTCTAGGATTGCTCGTCGAGGAGAAATGTGGGCTAAATGATGTTAATAACTTAATATTACTGTTGTCCTGCCTCAAGGACAACTCAAGGACCTGCTTTCAGATTTTAAGGGCCTTAAATGCATATCAAGGTTGGAACTTTCTCATTGAAAACGGAAGATGGGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NUC173

Protein Analysis

224

Amino Acids

24.83

Weight (kDa)

5.31

Isoelectric Point (pI)

43.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRP12_HEAT PF08161 72 - 176 4.5e-10 Ribosomal RNA-processing protein 12, HEAT repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 235
Acc36I ACCTGC 2 cut(s) 235, 608
AciI CCGC 4 cut(s) 17, 219, 337, 452
AcsI RAATTY 1 cut(s) 159
AcyI GRCGYC 1 cut(s) 216
AfaI GTAC 1 cut(s) 180
AfiI CCNNNNNNNGG 1 cut(s) 356
AgsI TTSAA 5 cut(s) 235, 296, 317, 387, 656
AluBI AGCT 2 cut(s) 52, 301
AluI AGCT 2 cut(s) 52, 301
AlwNI CAGNNNCTG 1 cut(s) 227
AoxI GGCC 2 cut(s) 498, 618
ApeKI GCWGC 1 cut(s) 221
ApoI RAATTY 1 cut(s) 159
ArsI GACNNNNNNTTYG 2 cut(s) 356, 388
AspS9I GGNCC 3 cut(s) 364, 597, 618
AsuHPI GGTGA 1 cut(s) 326
AvaII GGWCC 2 cut(s) 364, 597
BaeGI GKGCMC 1 cut(s) 437
BbsI GAAGAC 1 cut(s) 483
BbvI GCAGC 1 cut(s) 233
BccI CCATC 1 cut(s) 660
BclI TGATCA 1 cut(s) 270
BfaI CTAG 1 cut(s) 512
BfmI CTRYAG 1 cut(s) 288
BfuAI ACCTGC 2 cut(s) 235, 608
BisI GCNGC 2 cut(s) 219, 222
BlsI GCNGC 2 cut(s) 220, 223
Bme18I GGWCC 2 cut(s) 364, 597
BmgT120I GGNCC 3 cut(s) 364, 597, 618
BmiI GGNNCC 2 cut(s) 27, 282
BmsI GCATC 2 cut(s) 139, 264
BpiI GAAGAC 1 cut(s) 483
BpmI CTGGAG 1 cut(s) 461
BpuEI CTTGAG 2 cut(s) 566, 577
Bsa29I ATCGAT 1 cut(s) 258
BsaHI GRCGYC 1 cut(s) 216
BsaXI ACNNNNNCTCC 2 cut(s) 331, 361
Bsc4I CCNNNNNNNGG 1 cut(s) 356
BseCI ATCGAT 1 cut(s) 258
BseGI GGATG 1 cut(s) 104
BseLI CCNNNNNNNGG 1 cut(s) 356
BseRI GAGGAG 1 cut(s) 542
BseSI GKGCMC 1 cut(s) 437
BseXI GCAGC 1 cut(s) 233
BshFI GGCC 2 cut(s) 500, 620
BshVI ATCGAT 1 cut(s) 258
BslI CCNNNNNNNGG 1 cut(s) 356
BsmI GAATGC 1 cut(s) 230
BsnI GGCC 2 cut(s) 500, 620
Bsp1286I GDGCHC 1 cut(s) 437
Bsp1407I TGTACA 1 cut(s) 178
Bsp143I GATC 2 cut(s) 259, 270
BspACI CCGC 4 cut(s) 17, 219, 337, 452
BspANI GGCC 2 cut(s) 500, 620
BspDI ATCGAT 1 cut(s) 258
BspHI TCATGA 1 cut(s) 426
BspLI GGNNCC 2 cut(s) 27, 282
BspMI ACCTGC 2 cut(s) 235, 608
BsrGI TGTACA 1 cut(s) 178
BssMI GATC 2 cut(s) 259, 270
BssNI GRCGYC 1 cut(s) 216
Bst4CI ACNGT 2 cut(s) 122, 570
Bst6I CTCTTC 2 cut(s) 198, 510
BstACI GRCGYC 1 cut(s) 216
BstAPI GCANNNNNTGC 2 cut(s) 227, 466
BstAUI TGTACA 1 cut(s) 178
BstC8I GCNNGC 3 cut(s) 253, 437, 498
BstDEI CTNAG 1 cut(s) 48
BstF5I GGATG 1 cut(s) 104
BstKTI GATC 2 cut(s) 262, 273
BstMBI GATC 2 cut(s) 259, 270
BstMWI GCNNNNNNNGC 3 cut(s) 227, 466, 626
BstNSI RCATGY 1 cut(s) 460
BstSFI CTRYAG 1 cut(s) 288
BstSLI GKGCMC 1 cut(s) 437
BstV1I GCAGC 1 cut(s) 233
BstV2I GAAGAC 1 cut(s) 483
Bsu15I ATCGAT 1 cut(s) 258
BsuRI GGCC 2 cut(s) 500, 620
BsuTUI ATCGAT 1 cut(s) 258
BtsCI GGATG 1 cut(s) 104
BveI ACCTGC 2 cut(s) 235, 608
Cac8I GCNNGC 3 cut(s) 253, 437, 498
CaiI CAGNNNCTG 1 cut(s) 227
CciI TCATGA 1 cut(s) 426
Cfr13I GGNCC 3 cut(s) 364, 597, 618
ClaI ATCGAT 1 cut(s) 258
CseI GACGC 1 cut(s) 224
Csp6I GTAC 1 cut(s) 179
CviAII CATG 4 cut(s) 248, 361, 427, 457
CviJI RGCY 9 cut(s) 28, 52, 57, 267, 283, 301, 500, 541, 620
CviKI_1 RGCY 9 cut(s) 28, 52, 57, 267, 283, 301, 500, 541, 620
CviQI GTAC 1 cut(s) 179
DdeI CTNAG 1 cut(s) 48
DpnI GATC 2 cut(s) 261, 272
DpnII GATC 2 cut(s) 259, 270
Eam1104I CTCTTC 2 cut(s) 198, 510
EarI CTCTTC 2 cut(s) 198, 510
EciI GGCGGA 1 cut(s) 467
Eco47I GGWCC 2 cut(s) 364, 597
EcoO109I RGGNCCY 2 cut(s) 597, 618
EcoT22I ATGCAT 1 cut(s) 631
FaeI CATG 4 cut(s) 251, 364, 430, 460
FaiI YATR 9 cut(s) 60, 200, 249, 290, 362, 412, 428, 458, 631
FatI CATG 4 cut(s) 247, 360, 426, 456
FauI CCCGC 1 cut(s) 10
FbaI TGATCA 1 cut(s) 270
Fnu4HI GCNGC 2 cut(s) 219, 222
FokI GGATG 1 cut(s) 91
Fsp4HI GCNGC 2 cut(s) 219, 222
FspBI CTAG 1 cut(s) 512
GluI GCNGC 2 cut(s) 219, 222
GsuI CTGGAG 1 cut(s) 461
HaeIII GGCC 2 cut(s) 500, 620
HgaI GACGC 1 cut(s) 224
Hin1I GRCGYC 1 cut(s) 216
Hin1II CATG 4 cut(s) 251, 364, 430, 460
HphI GGTGA 1 cut(s) 326
Hpy166II GTNNAC 2 cut(s) 131, 323
Hpy188I TCNGA 1 cut(s) 609
Hpy188III TCNNGA 1 cut(s) 427
Hpy8I GTNNAC 2 cut(s) 131, 323
Hpy99I CGWCG 1 cut(s) 527
HpyCH4III ACNGT 2 cut(s) 122, 570
HpyCH4IV ACGT 1 cut(s) 65
HpyCH4V TGCA 7 cut(s) 62, 152, 230, 255, 371, 460, 629
HpyF10VI GCNNNNNNNGC 3 cut(s) 227, 466, 626
HpyF3I CTNAG 1 cut(s) 48
HpySE526I ACGT 1 cut(s) 65
Hsp92I GRCGYC 1 cut(s) 216
Hsp92II CATG 4 cut(s) 251, 364, 430, 460
Ksp22I TGATCA 1 cut(s) 270
Kzo9I GATC 2 cut(s) 259, 270
LmnI GCTCC 3 cut(s) 25, 280, 352
LpnPI CCDG 8 cut(s) 97, 189, 240, 363, 425, 482, 589, 613
Lsp1109I GCAGC 1 cut(s) 233
LweI GCATC 2 cut(s) 139, 264
MaeI CTAG 1 cut(s) 512
MaeII ACGT 1 cut(s) 65
MalI GATC 2 cut(s) 261, 272
MboI GATC 2 cut(s) 259, 270
MboII GAAGA 5 cut(s) 215, 488, 497, 500, 675
MhlI GDGCHC 1 cut(s) 437
MluCI AATT 3 cut(s) 11, 159, 401
MmeI TCCRAC 2 cut(s) 132, 620
MnlI CCTC 7 cut(s) 147, 184, 199, 370, 423, 520, 590
Mph1103I ATGCAT 1 cut(s) 631
MseI TTAA 6 cut(s) 164, 552, 560, 614, 623, 673
MslI CAYNNNNRTG 1 cut(s) 235
Mva1269I GAATGC 1 cut(s) 230
MwoI GCNNNNNNNGC 3 cut(s) 227, 466, 626
NdeII GATC 2 cut(s) 259, 270
NlaIII CATG 4 cut(s) 251, 364, 430, 460
NlaIV GGNNCC 2 cut(s) 27, 282
NmeAIII GCCGAG 2 cut(s) 33, 336
NsiI ATGCAT 1 cut(s) 631
NspI RCATGY 1 cut(s) 460
PagI TCATGA 1 cut(s) 426
PaqCI CACCTGC 1 cut(s) 235
PctI GAATGC 1 cut(s) 230
PkrI GCNGC 2 cut(s) 220, 223
PpuMI RGGWCCY 1 cut(s) 597
Psp5II RGGWCCY 1 cut(s) 597
PspN4I GGNNCC 2 cut(s) 27, 282
PspPI GGNCC 3 cut(s) 364, 597, 618
PspPPI RGGWCCY 1 cut(s) 597
PstNI CAGNNNCTG 1 cut(s) 227
RsaI GTAC 1 cut(s) 180
RsaNI GTAC 1 cut(s) 179
RseI CAYNNNNRTG 1 cut(s) 235
SaqAI TTAA 6 cut(s) 164, 552, 560, 614, 623, 673
SatI GCNGC 2 cut(s) 219, 222
Sau3AI GATC 2 cut(s) 259, 270
Sau96I GGNCC 3 cut(s) 364, 597, 618
SduI GDGCHC 1 cut(s) 437
SetI ASST 9 cut(s) 54, 68, 178, 229, 303, 369, 381, 602, 640
SfaNI GCATC 2 cut(s) 139, 264
SfcI CTRYAG 1 cut(s) 288
SinI GGWCC 2 cut(s) 364, 597
SmiMI CAYNNNNRTG 1 cut(s) 235
SmlI CTYRAG 2 cut(s) 581, 592
SmoI CTYRAG 2 cut(s) 581, 592
Sse9I AATT 3 cut(s) 11, 159, 401
SsiI CCGC 4 cut(s) 17, 219, 337, 452
SspI AATATT 1 cut(s) 564
SspMI CTAG 1 cut(s) 512
TaaI ACNGT 2 cut(s) 122, 570
TaiI ACGT 1 cut(s) 68
TaqI TCGA 3 cut(s) 258, 396, 525
TasI AATT 3 cut(s) 11, 159, 401
TatI WGTACW 1 cut(s) 178
TauI GCSGC 1 cut(s) 221
Tru1I TTAA 6 cut(s) 164, 552, 560, 614, 623, 673
Tru9I TTAA 6 cut(s) 164, 552, 560, 614, 623, 673
TseI GCWGC 1 cut(s) 221
TspDTI ATGAA 5 cut(s) 56, 93, 236, 415, 489
TspGWI ACGGA 1 cut(s) 675
VpaK11BI GGWCC 2 cut(s) 364, 597
XapI RAATTY 1 cut(s) 159
XceI RCATGY 1 cut(s) 460
XspI CTAG 1 cut(s) 512
Zsp2I ATGCAT 1 cut(s) 631
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.