Rroxscaffold_2G00146700

RRP12-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000835
Physical Location & Seq
Reverse (-)
84576282 .. 84579045
2764 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00146700.1

Sequence Viewer

Length: 813 bp
ATGATGTACATCCCAGAAACTGCAGCTGGTTTGCTCAGTATTGGAGTGGCGAAAGTCTATTCCTTTAACCAGAAAATCTGCGAAACTAATCTCCCTATTGTGTTTGATGCACTCAAAGTTATATTGGGAGAGCCTAAAATAGACGAACTTCCTGATGCAGTGCATGCATTTAAGTGTCTAATTCATGCTTGTGTTGATGAAAGCTTGATCAAAGAGGGAGTTGACTCTGTGGTGATGAGTGCAAGTATGGATGATGGAAGCAAGTCTGAGGCAACTATAATTGAAAAATTGTGTGCTACTATTCAAAGCCTACTTGGTCATCGCAAAACTACTATCTGGGCCCTTGCTTTTGAAGTTGTTTCAACAATGTTCGATAAATTAGGGGTATACTCTTCTTATTTGATGAGGGGTACACTCAAGACCTTGGCAGACATGCAGAAATTGCCTGATCAAGAATTTTCTTTCAAGGAAGAGGTTAAGCTTCTTCTAAAAATGCTTGTTGAGAAATGTGGGTTTGATGCTATAATGGCCGCCATGCCTCAAGAACACAAGAAACTGCTTCTAGAGATCAATGAAATGGGTTCTAAATCTGTGGAAGTGATACCTCATGCTTCAGAAGCAACTACAGTCAGGTCCGAAAGTAGTGACAATCAAATGCATGATAAAGCACTTGGACCATGCAAGAATTCAAAACGGAAAATGGATGGTGGAGGTAATGAGTTTTGTGATCGGCATGCCAGGAGTATTGCTAAGAGGGAAATGACAAAAAGGATGTCAGGTGCAGCTCTGATGGCGAAGAAGCTAAAACTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NUC173

Protein Analysis

270

Amino Acids

29.82

Weight (kDa)

7.04

Isoelectric Point (pI)

37.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRP12_HEAT PF08161 53 - 183 3e-18 Ribosomal RNA-processing protein 12, HEAT repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 387
AciI CCGC 1 cut(s) 531
AcoI YGGCCR 1 cut(s) 528
AcsI RAATTY 2 cut(s) 455, 685
AcuI CTGAAG 1 cut(s) 597
AfaI GTAC 2 cut(s) 8, 412
AgsI TTSAA 6 cut(s) 284, 305, 353, 363, 466, 690
AjnI CCWGG 1 cut(s) 737
AluBI AGCT 5 cut(s) 26, 204, 481, 785, 802
AluI AGCT 5 cut(s) 26, 204, 481, 785, 802
AlwNI CAGNNNCTG 1 cut(s) 20
AoxI GGCC 2 cut(s) 339, 528
ApaI GGGCCC 1 cut(s) 343
ApeKI GCWGC 2 cut(s) 23, 782
ApoI RAATTY 2 cut(s) 455, 685
ArsI GACNNNNNNTTYG 2 cut(s) 758, 790
AspS9I GGNCC 4 cut(s) 339, 340, 633, 674
AsuHPI GGTGA 1 cut(s) 244
AvaII GGWCC 2 cut(s) 633, 674
BaeGI GKGCMC 1 cut(s) 343
BanII GRGCYC 1 cut(s) 343
BbvI GCAGC 2 cut(s) 35, 794
BccI CCATC 3 cut(s) 248, 698, 784
BciT130I CCWGG 1 cut(s) 739
BclI TGATCA 2 cut(s) 207, 448
BfaI CTAG 2 cut(s) 563, 811
BfmI CTRYAG 2 cut(s) 21, 624
BisI GCNGC 3 cut(s) 24, 531, 783
BlsI GCNGC 3 cut(s) 25, 532, 784
Bme1390I CCNGG 1 cut(s) 739
Bme18I GGWCC 2 cut(s) 633, 674
BmgT120I GGNCC 4 cut(s) 339, 340, 633, 674
BmiI GGNNCC 1 cut(s) 341
BmrFI CCNGG 1 cut(s) 739
BmsI GCATC 3 cut(s) 97, 145, 508
BpuEI CTTGAG 2 cut(s) 401, 525
BsaBI GATNNNNATC 1 cut(s) 8
BsaJI CCNNGG 1 cut(s) 423
Bse8I GATNNNNATC 1 cut(s) 8
BseBI CCWGG 1 cut(s) 739
BseDI CCNNGG 1 cut(s) 423
BseGI GGATG 4 cut(s) 9, 256, 709, 777
BseJI GATNNNNATC 1 cut(s) 8
BseMII CTCAG 2 cut(s) 49, 258
BseSI GKGCMC 1 cut(s) 343
BseXI GCAGC 2 cut(s) 35, 794
BsgI GTGCAG 1 cut(s) 801
BshFI GGCC 2 cut(s) 341, 530
BsnI GGCC 2 cut(s) 341, 530
Bsp120I GGGCCC 1 cut(s) 339
Bsp1286I GDGCHC 1 cut(s) 343
Bsp1407I TGTACA 1 cut(s) 6
Bsp143I GATC 4 cut(s) 207, 448, 567, 727
BspACI CCGC 1 cut(s) 531
BspANI GGCC 2 cut(s) 341, 530
BspCNI CTCAG 2 cut(s) 48, 259
BspLI GGNNCC 1 cut(s) 341
BspMAI CTGCAG 1 cut(s) 25
BsrGI TGTACA 1 cut(s) 6
BssECI CCNNGG 1 cut(s) 423
BssMI GATC 4 cut(s) 207, 448, 567, 727
BssNAI GTATAC 1 cut(s) 388
BssT1I CCWWGG 1 cut(s) 423
Bst1107I GTATAC 1 cut(s) 388
Bst2UI CCWGG 1 cut(s) 739
Bst4CI ACNGT 1 cut(s) 628
Bst6I CTCTTC 2 cut(s) 397, 465
BstAPI GCANNNNNTGC 2 cut(s) 164, 442
BstAUI TGTACA 1 cut(s) 6
BstC8I GCNNGC 2 cut(s) 165, 735
BstDEI CTNAG 3 cut(s) 35, 267, 750
BstF5I GGATG 4 cut(s) 9, 256, 709, 777
BstKTI GATC 4 cut(s) 210, 451, 570, 730
BstMBI GATC 4 cut(s) 207, 448, 567, 727
BstMWI GCNNNNNNNGC 5 cut(s) 164, 442, 527, 617, 791
BstNI CCWGG 1 cut(s) 739
BstNSI RCATGY 3 cut(s) 167, 436, 737
BstSCI CCNGG 1 cut(s) 737
BstSFI CTRYAG 2 cut(s) 21, 624
BstSLI GKGCMC 1 cut(s) 343
BstV1I GCAGC 2 cut(s) 35, 794
BstZ17I GTATAC 1 cut(s) 388
BsuRI GGCC 2 cut(s) 341, 530
BtgZI GCGATG 1 cut(s) 305
BtsCI GGATG 4 cut(s) 9, 256, 709, 777
BtsI GCAGTG 1 cut(s) 165
BtsIMutI CAGTG 1 cut(s) 165
Cac8I GCNNGC 2 cut(s) 165, 735
CaiI CAGNNNCTG 1 cut(s) 20
Cfr13I GGNCC 4 cut(s) 339, 340, 633, 674
Csp6I GTAC 2 cut(s) 7, 411
CviAII CATG 8 cut(s) 164, 185, 433, 535, 608, 659, 678, 734
CviJI RGCY 9 cut(s) 26, 133, 204, 309, 341, 481, 530, 785, 802
CviKI_1 RGCY 9 cut(s) 26, 133, 204, 309, 341, 481, 530, 785, 802
CviQI GTAC 2 cut(s) 7, 411
DdeI CTNAG 3 cut(s) 35, 267, 750
DpnI GATC 4 cut(s) 209, 450, 569, 729
DpnII GATC 4 cut(s) 207, 448, 567, 727
EaeI YGGCCR 1 cut(s) 528
Eam1104I CTCTTC 2 cut(s) 397, 465
EarI CTCTTC 2 cut(s) 397, 465
Eco130I CCWWGG 1 cut(s) 423
Eco24I GRGCYC 1 cut(s) 343
Eco47I GGWCC 2 cut(s) 633, 674
Eco57I CTGAAG 1 cut(s) 597
EcoO109I RGGNCCY 1 cut(s) 340
EcoRI GAATTC 1 cut(s) 685
EcoRII CCWGG 1 cut(s) 737
EcoT14I CCWWGG 1 cut(s) 423
EcoT22I ATGCAT 2 cut(s) 169, 660
EcoT38I GRGCYC 1 cut(s) 343
ErhI CCWWGG 1 cut(s) 423
FaeI CATG 8 cut(s) 167, 188, 436, 538, 611, 662, 681, 737
FatI CATG 8 cut(s) 163, 184, 432, 534, 607, 658, 677, 733
FbaI TGATCA 2 cut(s) 207, 448
FblI GTMKAC 1 cut(s) 387
Fnu4HI GCNGC 3 cut(s) 24, 531, 783
FokI GGATG 3 cut(s) 263, 716, 784
FriOI GRGCYC 1 cut(s) 343
Fsp4HI GCNGC 3 cut(s) 24, 531, 783
FspBI CTAG 2 cut(s) 563, 811
GluI GCNGC 3 cut(s) 24, 531, 783
HaeIII GGCC 2 cut(s) 341, 530
Hin1II CATG 8 cut(s) 167, 188, 436, 538, 611, 662, 681, 737
HincII GTYRAC 1 cut(s) 223
HindII GTYRAC 1 cut(s) 223
HindIII AAGCTT 2 cut(s) 202, 479
HinfI GANTC 1 cut(s) 224
HphI GGTGA 1 cut(s) 244
Hpy166II GTNNAC 3 cut(s) 223, 388, 413
Hpy188I TCNGA 4 cut(s) 268, 616, 637, 789
Hpy188III TCNNGA 5 cut(s) 152, 418, 452, 542, 563
Hpy8I GTNNAC 3 cut(s) 223, 388, 413
HpyCH4III ACNGT 1 cut(s) 628
HpyF10VI GCNNNNNNNGC 5 cut(s) 164, 442, 527, 617, 791
HpyF3I CTNAG 3 cut(s) 35, 267, 750
Hsp92II CATG 8 cut(s) 167, 188, 436, 538, 611, 662, 681, 737
Ksp22I TGATCA 2 cut(s) 207, 448
Kzo9I GATC 4 cut(s) 207, 448, 567, 727
Lsp1109I GCAGC 2 cut(s) 35, 794
LweI GCATC 3 cut(s) 97, 145, 508
MaeI CTAG 2 cut(s) 563, 811
MaeIII GTNAC 1 cut(s) 644
MalI GATC 4 cut(s) 209, 450, 569, 729
MboI GATC 4 cut(s) 207, 448, 567, 727
MboII GAAGA 4 cut(s) 384, 476, 482, 808
MhlI GDGCHC 1 cut(s) 343
MluCI AATT 7 cut(s) 180, 279, 287, 377, 440, 455, 685
MlyI GAGTC 1 cut(s) 218
MnlI CCTC 8 cut(s) 208, 262, 399, 466, 549, 615, 704, 747
Mph1103I ATGCAT 2 cut(s) 169, 660
MseI TTAA 3 cut(s) 66, 171, 477
MslI CAYNNNNRTG 2 cut(s) 172, 189
MspA1I CMGCKG 1 cut(s) 26
MspR9I CCNGG 1 cut(s) 739
MvaI CCWGG 1 cut(s) 739
MwoI GCNNNNNNNGC 5 cut(s) 164, 442, 527, 617, 791
NdeII GATC 4 cut(s) 207, 448, 567, 727
NlaIII CATG 8 cut(s) 167, 188, 436, 538, 611, 662, 681, 737
NlaIV GGNNCC 1 cut(s) 341
NmuCI GTSAC 1 cut(s) 644
NsiI ATGCAT 2 cut(s) 169, 660
NspI RCATGY 3 cut(s) 167, 436, 737
PaeI GCATGC 2 cut(s) 167, 737
PkrI GCNGC 3 cut(s) 25, 532, 784
PleI GAGTC 1 cut(s) 218
PpsI GAGTC 1 cut(s) 218
Psp6I CCWGG 1 cut(s) 737
PspGI CCWGG 1 cut(s) 737
PspN4I GGNNCC 1 cut(s) 341
PspOMI GGGCCC 1 cut(s) 339
PspPI GGNCC 4 cut(s) 339, 340, 633, 674
PstI CTGCAG 1 cut(s) 25
PstNI CAGNNNCTG 1 cut(s) 20
PvuII CAGCTG 1 cut(s) 26
RsaI GTAC 2 cut(s) 8, 412
RsaNI GTAC 2 cut(s) 7, 411
RseI CAYNNNNRTG 2 cut(s) 172, 189
SaqAI TTAA 3 cut(s) 66, 171, 477
SatI GCNGC 3 cut(s) 24, 531, 783
Sau3AI GATC 4 cut(s) 207, 448, 567, 727
Sau96I GGNCC 4 cut(s) 339, 340, 633, 674
SchI GAGTC 1 cut(s) 218
ScrFI CCNGG 1 cut(s) 739
SduI GDGCHC 1 cut(s) 343
SfaNI GCATC 3 cut(s) 97, 145, 508
SfcI CTRYAG 2 cut(s) 21, 624
SinI GGWCC 2 cut(s) 633, 674
SmiMI CAYNNNNRTG 2 cut(s) 172, 189
SmlI CTYRAG 2 cut(s) 416, 540
SmoI CTYRAG 2 cut(s) 416, 540
SphI GCATGC 2 cut(s) 167, 737
Sse9I AATT 7 cut(s) 180, 279, 287, 377, 440, 455, 685
SsiI CCGC 1 cut(s) 531
SspMI CTAG 2 cut(s) 563, 811
StyD4I CCNGG 1 cut(s) 737
StyI CCWWGG 1 cut(s) 423
TaaI ACNGT 1 cut(s) 628
TaqI TCGA 1 cut(s) 372
TasI AATT 7 cut(s) 180, 279, 287, 377, 440, 455, 685
TatI WGTACW 1 cut(s) 6
TauI GCSGC 1 cut(s) 533
Tru1I TTAA 3 cut(s) 66, 171, 477
Tru9I TTAA 3 cut(s) 66, 171, 477
TscAI CASTG 1 cut(s) 165
TseFI GTSAC 1 cut(s) 644
TseI GCWGC 2 cut(s) 23, 782
Tsp45I GTSAC 1 cut(s) 644
TspDTI ATGAA 3 cut(s) 173, 213, 588
TspGWI ACGGA 1 cut(s) 709
TspRI CASTG 1 cut(s) 165
VpaK11BI GGWCC 2 cut(s) 633, 674
XapI RAATTY 2 cut(s) 455, 685
XbaI TCTAGA 1 cut(s) 562
XceI RCATGY 3 cut(s) 167, 436, 737
XmiI GTMKAC 1 cut(s) 387
XspI CTAG 2 cut(s) 563, 811
Zsp2I ATGCAT 2 cut(s) 169, 660
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.