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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 1 cut(s) | 24 |
| AcoI | YGGCCR | 1 cut(s) | 337 |
| AfaI | GTAC | 1 cut(s) | 74 |
| AgsI | TTSAA | 1 cut(s) | 387 |
| AluBI | AGCT | 4 cut(s) | 20, 125, 315, 350 |
| AluI | AGCT | 4 cut(s) | 20, 125, 315, 350 |
| Alw26I | GTCTC | 1 cut(s) | 281 |
| AoxI | GGCC | 4 cut(s) | 30, 159, 337, 356 |
| ApeKI | GCWGC | 2 cut(s) | 17, 20 |
| AspS9I | GGNCC | 2 cut(s) | 116, 257 |
| AvaII | GGWCC | 2 cut(s) | 116, 257 |
| BbvI | GCAGC | 2 cut(s) | 7, 29 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 229, 263 |
| BciVI | GTATCC | 1 cut(s) | 420 |
| BcoDI | GTCTC | 1 cut(s) | 281 |
| BfaI | CTAG | 1 cut(s) | 347 |
| BfmI | CTRYAG | 1 cut(s) | 324 |
| BfuI | GTATCC | 1 cut(s) | 420 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 29 |
| BisI | GCNGC | 3 cut(s) | 18, 21, 24 |
| BlsI | GCNGC | 3 cut(s) | 19, 22, 25 |
| Bme18I | GGWCC | 2 cut(s) | 116, 257 |
| BmgT120I | GGNCC | 2 cut(s) | 116, 257 |
| BmiI | GGNNCC | 2 cut(s) | 117, 259 |
| BpmI | CTGGAG | 1 cut(s) | 359 |
| Bpu10I | CCTNAGC | 1 cut(s) | 27 |
| BsaJI | CCNNGG | 1 cut(s) | 111 |
| Bse1I | ACTGG | 2 cut(s) | 142, 340 |
| BseDI | CCNNGG | 1 cut(s) | 111 |
| BseMII | CTCAG | 1 cut(s) | 147 |
| BseNI | ACTGG | 2 cut(s) | 142, 340 |
| BseXI | GCAGC | 2 cut(s) | 7, 29 |
| BseYI | CCCAGC | 1 cut(s) | 350 |
| BshFI | GGCC | 4 cut(s) | 32, 161, 339, 358 |
| BslFI | GGGAC | 1 cut(s) | 243 |
| BsmAI | GTCTC | 1 cut(s) | 281 |
| BsmFI | GGGAC | 1 cut(s) | 243 |
| BsnI | GGCC | 4 cut(s) | 32, 161, 339, 358 |
| BspACI | CCGC | 1 cut(s) | 24 |
| BspANI | GGCC | 4 cut(s) | 32, 161, 339, 358 |
| BspCNI | CTCAG | 1 cut(s) | 148 |
| BspLI | GGNNCC | 2 cut(s) | 117, 259 |
| BsrI | ACTGG | 2 cut(s) | 142, 340 |
| BssECI | CCNNGG | 1 cut(s) | 111 |
| Bst4CI | ACNGT | 1 cut(s) | 185 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 401 |
| BstDEI | CTNAG | 2 cut(s) | 27, 156 |
| BstDSI | CCRYGG | 1 cut(s) | 111 |
| BstMAI | GTCTC | 1 cut(s) | 281 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 23, 29, 43, 401 |
| BstNSI | RCATGY | 1 cut(s) | 292 |
| BstSFI | CTRYAG | 1 cut(s) | 324 |
| BstV1I | GCAGC | 2 cut(s) | 7, 29 |
| BsuI | GTATCC | 1 cut(s) | 420 |
| BsuRI | GGCC | 4 cut(s) | 32, 161, 339, 358 |
| BtgI | CCRYGG | 1 cut(s) | 111 |
| BtsIMutI | CAGTG | 1 cut(s) | 190 |
| Cfr13I | GGNCC | 2 cut(s) | 116, 257 |
| Csp6I | GTAC | 1 cut(s) | 73 |
| CviAII | CATG | 3 cut(s) | 268, 289, 368 |
| CviJI | RGCY | 8 cut(s) | 20, 32, 125, 161, 315, 339, 350, 358 |
| CviKI_1 | RGCY | 8 cut(s) | 20, 32, 125, 161, 315, 339, 350, 358 |
| CviQI | GTAC | 1 cut(s) | 73 |
| DdeI | CTNAG | 2 cut(s) | 27, 156 |
| DraI | TTTAAA | 1 cut(s) | 130 |
| EaeI | YGGCCR | 1 cut(s) | 337 |
| Eco47I | GGWCC | 2 cut(s) | 116, 257 |
| FaeI | CATG | 3 cut(s) | 271, 292, 371 |
| FaiI | YATR | 8 cut(s) | 60, 224, 236, 269, 290, 326, 369, 400 |
| FaqI | GGGAC | 1 cut(s) | 243 |
| FatI | CATG | 3 cut(s) | 267, 288, 367 |
| Fnu4HI | GCNGC | 3 cut(s) | 18, 21, 24 |
| Fsp4HI | GCNGC | 3 cut(s) | 18, 21, 24 |
| FspBI | CTAG | 1 cut(s) | 347 |
| GluI | GCNGC | 3 cut(s) | 18, 21, 24 |
| GsaI | CCCAGC | 1 cut(s) | 354 |
| GsuI | CTGGAG | 1 cut(s) | 359 |
| HaeIII | GGCC | 4 cut(s) | 32, 161, 339, 358 |
| Hin1II | CATG | 3 cut(s) | 271, 292, 371 |
| Hpy166II | GTNNAC | 1 cut(s) | 75 |
| Hpy188I | TCNGA | 3 cut(s) | 157, 297, 421 |
| Hpy188III | TCNNGA | 3 cut(s) | 260, 376, 387 |
| Hpy8I | GTNNAC | 1 cut(s) | 75 |
| Hpy99I | CGWCG | 1 cut(s) | 266 |
| HpyAV | CCTTC | 3 cut(s) | 114, 372, 373 |
| HpyCH4III | ACNGT | 1 cut(s) | 185 |
| HpyCH4V | TGCA | 1 cut(s) | 404 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 23, 29, 43, 401 |
| HpyF3I | CTNAG | 2 cut(s) | 27, 156 |
| Hsp92II | CATG | 3 cut(s) | 271, 292, 371 |
| LpnPI | CCDG | 4 cut(s) | 123, 336, 353, 389 |
| Lsp1109I | GCAGC | 2 cut(s) | 7, 29 |
| MaeI | CTAG | 1 cut(s) | 347 |
| MaeIII | GTNAC | 1 cut(s) | 179 |
| MboII | GAAGA | 1 cut(s) | 205 |
| MluCI | AATT | 2 cut(s) | 131, 141 |
| MseI | TTAA | 2 cut(s) | 129, 144 |
| MspA1I | CMGCKG | 1 cut(s) | 20 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 23, 29, 43, 401 |
| NlaIII | CATG | 3 cut(s) | 271, 292, 371 |
| NlaIV | GGNNCC | 2 cut(s) | 117, 259 |
| NmeAIII | GCCGAG | 1 cut(s) | 214 |
| NmuCI | GTSAC | 1 cut(s) | 179 |
| NspI | RCATGY | 1 cut(s) | 292 |
| PkrI | GCNGC | 3 cut(s) | 19, 22, 25 |
| PspFI | CCCAGC | 1 cut(s) | 350 |
| PspN4I | GGNNCC | 2 cut(s) | 117, 259 |
| PspPI | GGNCC | 2 cut(s) | 116, 257 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 56, 88 |
| PvuII | CAGCTG | 1 cut(s) | 20 |
| RsaI | GTAC | 1 cut(s) | 74 |
| RsaNI | GTAC | 1 cut(s) | 73 |
| SaqAI | TTAA | 2 cut(s) | 129, 144 |
| SatI | GCNGC | 3 cut(s) | 18, 21, 24 |
| Sau96I | GGNCC | 2 cut(s) | 116, 257 |
| SfcI | CTRYAG | 1 cut(s) | 324 |
| SinI | GGWCC | 2 cut(s) | 116, 257 |
| Sse9I | AATT | 2 cut(s) | 131, 141 |
| SsiI | CCGC | 1 cut(s) | 24 |
| SspMI | CTAG | 1 cut(s) | 347 |
| TaaI | ACNGT | 1 cut(s) | 185 |
| TaqII | GACCGA | 1 cut(s) | 318 |
| TasI | AATT | 2 cut(s) | 131, 141 |
| TauI | GCSGC | 1 cut(s) | 26 |
| Tru1I | TTAA | 2 cut(s) | 129, 144 |
| Tru9I | TTAA | 2 cut(s) | 129, 144 |
| TscAI | CASTG | 1 cut(s) | 190 |
| TseFI | GTSAC | 1 cut(s) | 179 |
| TseI | GCWGC | 2 cut(s) | 17, 20 |
| Tsp45I | GTSAC | 1 cut(s) | 179 |
| TspDTI | ATGAA | 1 cut(s) | 17 |
| TspGWI | ACGGA | 1 cut(s) | 108 |
| TspRI | CASTG | 1 cut(s) | 190 |
| VpaK11BI | GGWCC | 2 cut(s) | 116, 257 |
| XceI | RCATGY | 1 cut(s) | 292 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 347 |
| XspI | CTAG | 1 cut(s) | 347 |