Belongs to the iron ascorbate-dependent oxidoreductase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AcoI | YGGCCR | 1 cut(s) | 65 |
| AcsI | RAATTY | 2 cut(s) | 114, 322 |
| AfaI | GTAC | 1 cut(s) | 176 |
| AflIII | ACRYGT | 1 cut(s) | 430 |
| AluBI | AGCT | 3 cut(s) | 53, 158, 183 |
| AluI | AGCT | 3 cut(s) | 53, 158, 183 |
| Alw26I | GTCTC | 2 cut(s) | 93, 126 |
| AoxI | GGCC | 1 cut(s) | 65 |
| ApeKI | GCWGC | 1 cut(s) | 183 |
| ApoI | RAATTY | 2 cut(s) | 114, 322 |
| AseI | ATTAAT | 1 cut(s) | 351 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 40 |
| BbvI | GCAGC | 1 cut(s) | 170 |
| BccI | CCATC | 2 cut(s) | 76, 336 |
| BceAI | ACGGC | 1 cut(s) | 80 |
| BcoDI | GTCTC | 2 cut(s) | 93, 126 |
| BisI | GCNGC | 1 cut(s) | 184 |
| BlsI | GCNGC | 1 cut(s) | 185 |
| BmsI | GCATC | 1 cut(s) | 87 |
| BpuEI | CTTGAG | 3 cut(s) | 170, 302, 341 |
| Bse3DI | GCAATG | 1 cut(s) | 172 |
| BseGI | GGATG | 3 cut(s) | 68, 177, 295 |
| BseMI | GCAATG | 1 cut(s) | 172 |
| BseXI | GCAGC | 1 cut(s) | 170 |
| BseYI | CCCAGC | 1 cut(s) | 49 |
| BshFI | GGCC | 1 cut(s) | 67 |
| BsmAI | GTCTC | 2 cut(s) | 93, 126 |
| BsnI | GGCC | 1 cut(s) | 67 |
| Bsp1407I | TGTACA | 1 cut(s) | 174 |
| BspANI | GGCC | 1 cut(s) | 67 |
| BsrDI | GCAATG | 1 cut(s) | 172 |
| BsrGI | TGTACA | 1 cut(s) | 174 |
| Bst4CI | ACNGT | 1 cut(s) | 217 |
| BstAUI | TGTACA | 1 cut(s) | 174 |
| BstF5I | GGATG | 3 cut(s) | 68, 177, 295 |
| BstMAI | GTCTC | 2 cut(s) | 93, 126 |
| BstNSI | RCATGY | 1 cut(s) | 434 |
| BstV1I | GCAGC | 1 cut(s) | 170 |
| BsuRI | GGCC | 1 cut(s) | 67 |
| BtsCI | GGATG | 3 cut(s) | 68, 177, 295 |
| Csp6I | GTAC | 1 cut(s) | 175 |
| CviAII | CATG | 3 cut(s) | 407, 431, 449 |
| CviJI | RGCY | 7 cut(s) | 47, 53, 67, 158, 183, 197, 302 |
| CviKI_1 | RGCY | 7 cut(s) | 47, 53, 67, 158, 183, 197, 302 |
| CviQI | GTAC | 1 cut(s) | 175 |
| EaeI | YGGCCR | 1 cut(s) | 65 |
| EcoRI | GAATTC | 1 cut(s) | 114 |
| FaeI | CATG | 3 cut(s) | 410, 434, 452 |
| FaiI | YATR | 7 cut(s) | 126, 293, 395, 408, 432, 450, 454 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 170, 202 |
| FatI | CATG | 3 cut(s) | 406, 430, 448 |
| Fnu4HI | GCNGC | 1 cut(s) | 184 |
| FokI | GGATG | 3 cut(s) | 55, 184, 282 |
| Fsp4HI | GCNGC | 1 cut(s) | 184 |
| GluI | GCNGC | 1 cut(s) | 184 |
| GsaI | CCCAGC | 1 cut(s) | 53 |
| HaeIII | GGCC | 1 cut(s) | 67 |
| Hin1II | CATG | 3 cut(s) | 410, 434, 452 |
| HinfI | GANTC | 2 cut(s) | 105, 333 |
| Hpy166II | GTNNAC | 1 cut(s) | 205 |
| Hpy188III | TCNNGA | 2 cut(s) | 149, 319 |
| Hpy8I | GTNNAC | 1 cut(s) | 205 |
| HpyAV | CCTTC | 1 cut(s) | 250 |
| HpyCH4III | ACNGT | 1 cut(s) | 217 |
| HpyCH4IV | ACGT | 1 cut(s) | 22 |
| HpyCH4V | TGCA | 3 cut(s) | 27, 78, 165 |
| HpySE526I | ACGT | 1 cut(s) | 22 |
| Hsp92II | CATG | 3 cut(s) | 410, 434, 452 |
| LpnPI | CCDG | 6 cut(s) | 63, 178, 312, 390, 398, 457 |
| Lsp1109I | GCAGC | 1 cut(s) | 170 |
| LweI | GCATC | 1 cut(s) | 87 |
| MaeII | ACGT | 1 cut(s) | 22 |
| MaeIII | GTNAC | 2 cut(s) | 18, 433 |
| MfeI | CAATTG | 1 cut(s) | 13 |
| MluCI | AATT | 4 cut(s) | 13, 82, 114, 322 |
| MlyI | GAGTC | 1 cut(s) | 114 |
| MmeI | TCCRAC | 2 cut(s) | 304, 326 |
| MnlI | CCTC | 2 cut(s) | 326, 350 |
| MroXI | GAANNNNTTC | 1 cut(s) | 40 |
| MseI | TTAA | 3 cut(s) | 93, 351, 402 |
| MslI | CAYNNNNRTG | 1 cut(s) | 453 |
| MspA1I | CMGCKG | 1 cut(s) | 53 |
| MunI | CAATTG | 1 cut(s) | 13 |
| NlaIII | CATG | 3 cut(s) | 410, 434, 452 |
| NmuCI | GTSAC | 1 cut(s) | 433 |
| NspI | RCATGY | 1 cut(s) | 434 |
| PciI | ACATGT | 1 cut(s) | 430 |
| PdmI | GAANNNNTTC | 1 cut(s) | 40 |
| PfeI | GAWTC | 1 cut(s) | 333 |
| PkrI | GCNGC | 1 cut(s) | 185 |
| PleI | GAGTC | 1 cut(s) | 113 |
| PpsI | GAGTC | 1 cut(s) | 113 |
| PscI | ACATGT | 1 cut(s) | 430 |
| PshBI | ATTAAT | 1 cut(s) | 351 |
| PspFI | CCCAGC | 1 cut(s) | 49 |
| PvuII | CAGCTG | 1 cut(s) | 53 |
| RsaI | GTAC | 1 cut(s) | 176 |
| RsaNI | GTAC | 1 cut(s) | 175 |
| RseI | CAYNNNNRTG | 1 cut(s) | 453 |
| SaqAI | TTAA | 3 cut(s) | 93, 351, 402 |
| SatI | GCNGC | 1 cut(s) | 184 |
| SchI | GAGTC | 1 cut(s) | 114 |
| SetI | ASST | 8 cut(s) | 25, 55, 160, 185, 286, 318, 342, 446 |
| SfaNI | GCATC | 1 cut(s) | 87 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 453 |
| SmlI | CTYRAG | 3 cut(s) | 149, 317, 356 |
| SmoI | CTYRAG | 3 cut(s) | 149, 317, 356 |
| Sse9I | AATT | 4 cut(s) | 13, 82, 114, 322 |
| TaaI | ACNGT | 1 cut(s) | 217 |
| TaiI | ACGT | 1 cut(s) | 25 |
| TaqI | TCGA | 1 cut(s) | 331 |
| TasI | AATT | 4 cut(s) | 13, 82, 114, 322 |
| TatI | WGTACW | 1 cut(s) | 174 |
| TfiI | GAWTC | 1 cut(s) | 333 |
| Tru1I | TTAA | 3 cut(s) | 93, 351, 402 |
| Tru9I | TTAA | 3 cut(s) | 93, 351, 402 |
| TseFI | GTSAC | 1 cut(s) | 433 |
| TseI | GCWGC | 1 cut(s) | 183 |
| Tsp45I | GTSAC | 1 cut(s) | 433 |
| TspGWI | ACGGA | 1 cut(s) | 332 |
| VspI | ATTAAT | 1 cut(s) | 351 |
| XapI | RAATTY | 2 cut(s) | 114, 322 |
| XceI | RCATGY | 1 cut(s) | 434 |
| XmnI | GAANNNNTTC | 1 cut(s) | 40 |