Rroxscaffold_1G00074020

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0002282
Physical Location & Seq
Reverse (-)
94944977 .. 94952601
7625 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00074020.1

Sequence Viewer

Length: 711 bp
ATGTCGGGAAAGAGACCTGCTAATGGTTACTTAGAAGAAACATCGAGATGGATCCATCTTGTTCAAATCGGAGAATTCATTGGGAAAGAAAAGCAAATCACTATTACACAATGGAGAGTGTTGATGTTGTCCGGAGCGAGTGGGGAAAGAGGGCCCCGCGACATGGTAGGTCCCGCCTACGATATGCATCCGAGAGACCGACACTCGAGCTACCTCGCCATGGTGGAGGTCGGCCGGTATCTTGCTAGGAGGCCACCCTCCCGTGCTCTCCAAAAGCCTTCAAGAGAAGACACCCCGATGATCTCCGACACGAGCGATACTACCTGGCAATCCGCTACCACTCTAGCTCGATTCCATCTAGGTACCAATTGGGTCTCCTCCGGGAACTCATCACGACCTTCTAAATTAGGTCACTGCATGCAACTTTCGTCAAATCTCGGTGGCCTAACTCCTGCTCTTAGTGGCCCCAATTCTTGGCGAAATTACTCTTGGTACTTCGGTATCTTCATCTCGCTATCCCCACTGAGATTCAAAAAAGCATGGGAGCTTCAAACATCCCGGTATCAGTCTCAGTCTTTCGCCTCATCCCGGCAACATCTTCATCTACCGCCTATCATCTCGAAAACTTTTTTACTTCCCGGATCACCACTAGCAGCCCCCTCACTTCCCAGTCAGGCACTACCCGCCGAGCATGCTCCGAGCACCGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

236

Amino Acids

26.29

Weight (kDa)

10.04

Isoelectric Point (pI)

57.26

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 25
Acc65I GGTACC 1 cut(s) 362
AccB1I GGYRCC 1 cut(s) 362
AccB7I CCANNNNNTGG 1 cut(s) 474
AccII CGCG 1 cut(s) 159
AccIII TCCGGA 1 cut(s) 131
AciI CCGC 5 cut(s) 157, 174, 333, 608, 684
AclWI GGATC 3 cut(s) 46, 59, 649
AcoI YGGCCR 1 cut(s) 232
AcsI RAATTY 1 cut(s) 74
AfaI GTAC 2 cut(s) 364, 494
AfiI CCNNNNNNNGG 5 cut(s) 23, 163, 220, 474, 588
AgsI TTSAA 4 cut(s) 65, 282, 532, 551
AjnI CCWGG 1 cut(s) 323
AjuI GAANNNNNNNTTGG 2 cut(s) 472, 504
AluBI AGCT 3 cut(s) 210, 347, 547
AluI AGCT 3 cut(s) 210, 347, 547
Alw21I GWGCWC 2 cut(s) 268, 704
Alw26I GTCTC 4 cut(s) 7, 189, 379, 573
AlwI GGATC 3 cut(s) 46, 59, 649
Ama87I CYCGRG 1 cut(s) 205
Aor13HI TCCGGA 1 cut(s) 131
AoxI GGCC 5 cut(s) 152, 232, 251, 442, 463
ApaI GGGCCC 1 cut(s) 156
ApeKI GCWGC 1 cut(s) 653
ApoI RAATTY 1 cut(s) 74
Asp718I GGTACC 1 cut(s) 362
AspS9I GGNCC 4 cut(s) 152, 153, 170, 464
AsuC2I CCSGG 4 cut(s) 382, 559, 589, 639
AsuHPI GGTGA 1 cut(s) 636
AvaI CYCGRG 1 cut(s) 205
AvaII GGWCC 1 cut(s) 170
BaeGI GKGCMC 1 cut(s) 156
BaeI ACNNNNGTAYC 4 cut(s) 484, 484, 517, 517
BamHI GGATCC 1 cut(s) 51
BanI GGYRCC 1 cut(s) 362
BanII GRGCYC 1 cut(s) 156
BauI CACGAG 1 cut(s) 310
BbsI GAAGAC 1 cut(s) 294
Bbv12I GWGCWC 2 cut(s) 268, 704
BbvI GCAGC 1 cut(s) 665
BccI CCATC 3 cut(s) 42, 63, 363
BciT130I CCWGG 1 cut(s) 325
BcnI CCSGG 4 cut(s) 382, 559, 589, 639
BcoDI GTCTC 4 cut(s) 7, 189, 379, 573
BfaI CTAG 5 cut(s) 246, 344, 359, 650, 709
BfuAI ACCTGC 1 cut(s) 25
BisI GCNGC 1 cut(s) 654
BlsI GCNGC 1 cut(s) 655
Bme1390I CCNGG 5 cut(s) 325, 382, 559, 589, 639
Bme18I GGWCC 1 cut(s) 170
BmeT110I CYCGRG 1 cut(s) 205
BmgT120I GGNCC 4 cut(s) 152, 153, 170, 464
BmiI GGNNCC 6 cut(s) 53, 154, 155, 172, 364, 466
BmrFI CCNGG 5 cut(s) 325, 382, 559, 589, 639
BmrI ACTGGG 1 cut(s) 663
BmsI GCATC 1 cut(s) 196
BmuI ACTGGG 1 cut(s) 663
BpiI GAAGAC 1 cut(s) 294
BpuMI CCSGG 4 cut(s) 382, 559, 589, 639
BsaBI GATNNNNATC 1 cut(s) 186
BsaI GGTCTC 3 cut(s) 7, 189, 379
BsaJI CCNNGG 1 cut(s) 219
BsaWI WCCGGW 1 cut(s) 131
Bsc4I CCNNNNNNNGG 5 cut(s) 23, 163, 220, 474, 588
Bse118I RCCGGY 1 cut(s) 234
Bse1I ACTGG 1 cut(s) 669
Bse8I GATNNNNATC 1 cut(s) 186
BseAI TCCGGA 1 cut(s) 131
BseBI CCWGG 1 cut(s) 325
BseDI CCNNGG 1 cut(s) 219
BseGI GGATG 3 cut(s) 187, 554, 584
BseJI GATNNNNATC 1 cut(s) 186
BseLI CCNNNNNNNGG 5 cut(s) 23, 163, 220, 474, 588
BseMII CTCAG 2 cut(s) 515, 584
BseNI ACTGG 1 cut(s) 669
BseRI GAGGAG 1 cut(s) 367
BseSI GKGCMC 1 cut(s) 156
BseX3I CGGCCG 1 cut(s) 232
BseXI GCAGC 1 cut(s) 665
Bsh1236I CGCG 1 cut(s) 159
Bsh1285I CGRYCG 1 cut(s) 235
BshFI GGCC 5 cut(s) 154, 234, 253, 444, 465
BshNI GGYRCC 1 cut(s) 362
BsiEI CGRYCG 1 cut(s) 235
BsiHKAI GWGCWC 2 cut(s) 268, 704
BsiHKCI CYCGRG 1 cut(s) 205
BsiSI CCGG 6 cut(s) 132, 235, 381, 559, 589, 639
BslFI GGGAC 1 cut(s) 156
BslI CCNNNNNNNGG 5 cut(s) 23, 163, 220, 474, 588
BsmAI GTCTC 4 cut(s) 7, 189, 379, 573
BsmFI GGGAC 1 cut(s) 156
BsnI GGCC 5 cut(s) 154, 234, 253, 444, 465
Bso31I GGTCTC 3 cut(s) 7, 189, 379
BsoBI CYCGRG 1 cut(s) 205
Bsp120I GGGCCC 1 cut(s) 152
Bsp1286I GDGCHC 3 cut(s) 156, 268, 704
Bsp13I TCCGGA 1 cut(s) 131
Bsp143I GATC 3 cut(s) 51, 300, 641
Bsp19I CCATGG 1 cut(s) 219
BspACI CCGC 5 cut(s) 157, 174, 333, 608, 684
BspANI GGCC 5 cut(s) 154, 234, 253, 444, 465
BspCNI CTCAG 2 cut(s) 516, 583
BspEI TCCGGA 1 cut(s) 131
BspFNI CGCG 1 cut(s) 159
BspLI GGNNCC 6 cut(s) 53, 154, 155, 172, 364, 466
BspMI ACCTGC 1 cut(s) 25
BspPI GGATC 3 cut(s) 46, 59, 649
BspT107I GGYRCC 1 cut(s) 362
BspTNI GGTCTC 3 cut(s) 7, 189, 379
BsrFI RCCGGY 1 cut(s) 234
BsrI ACTGG 1 cut(s) 669
BssAI RCCGGY 1 cut(s) 234
BssECI CCNNGG 1 cut(s) 219
BssMI GATC 3 cut(s) 51, 300, 641
BssSI CACGAG 1 cut(s) 310
BssT1I CCWWGG 1 cut(s) 219
Bst2BI CACGAG 1 cut(s) 310
Bst2UI CCWGG 1 cut(s) 325
BstC8I GCNNGC 2 cut(s) 419, 693
BstDEI CTNAG 4 cut(s) 31, 458, 524, 570
BstDSI CCRYGG 1 cut(s) 219
BstF5I GGATG 3 cut(s) 187, 554, 584
BstFNI CGCG 1 cut(s) 159
BstKTI GATC 3 cut(s) 54, 303, 644
BstMAI GTCTC 4 cut(s) 7, 189, 379, 573
BstMBI GATC 3 cut(s) 51, 300, 641
BstMCI CGRYCG 1 cut(s) 235
BstMWI GCNNNNNNNGC 2 cut(s) 683, 692
BstNI CCWGG 1 cut(s) 325
BstNSI RCATGY 2 cut(s) 421, 695
BstSCI CCNGG 5 cut(s) 323, 380, 557, 587, 637
BstSLI GKGCMC 1 cut(s) 156
BstUI CGCG 1 cut(s) 159
BstV1I GCAGC 1 cut(s) 665
BstV2I GAAGAC 1 cut(s) 294
BstX2I RGATCY 1 cut(s) 51
BstYI RGATCY 1 cut(s) 51
BstZI CGGCCG 1 cut(s) 232
BsuRI GGCC 5 cut(s) 154, 234, 253, 444, 465
BtgI CCRYGG 1 cut(s) 219
BtsCI GGATG 3 cut(s) 187, 554, 584
BtsI GCAGTG 1 cut(s) 412
BtsIMutI CAGTG 2 cut(s) 412, 521
BveI ACCTGC 1 cut(s) 25
Cac8I GCNNGC 2 cut(s) 419, 693
Cfr10I RCCGGY 1 cut(s) 234
Cfr13I GGNCC 4 cut(s) 152, 153, 170, 464
Csp6I GTAC 2 cut(s) 363, 493
CviAII CATG 5 cut(s) 163, 220, 418, 540, 692
CviQI GTAC 2 cut(s) 363, 493
DdeI CTNAG 4 cut(s) 31, 458, 524, 570
DpnI GATC 3 cut(s) 53, 302, 643
DpnII GATC 3 cut(s) 51, 300, 641
EaeI YGGCCR 1 cut(s) 232
EagI CGGCCG 1 cut(s) 232
EclXI CGGCCG 1 cut(s) 232
Eco130I CCWWGG 1 cut(s) 219
Eco24I GRGCYC 1 cut(s) 156
Eco31I GGTCTC 3 cut(s) 7, 189, 379
Eco47I GGWCC 1 cut(s) 170
Eco52I CGGCCG 1 cut(s) 232
Eco88I CYCGRG 1 cut(s) 205
EcoO109I RGGNCCY 3 cut(s) 152, 153, 170
EcoRI GAATTC 1 cut(s) 74
EcoRII CCWGG 1 cut(s) 323
EcoT14I CCWWGG 1 cut(s) 219
EcoT22I ATGCAT 1 cut(s) 189
EcoT38I GRGCYC 1 cut(s) 156
ErhI CCWWGG 1 cut(s) 219
FaeI CATG 5 cut(s) 166, 223, 421, 543, 695
FaiI YATR 6 cut(s) 164, 185, 221, 419, 541, 693
FaqI GGGAC 1 cut(s) 156
FatI CATG 5 cut(s) 162, 219, 417, 539, 691
FauI CCCGC 3 cut(s) 164, 181, 691
Fnu4HI GCNGC 1 cut(s) 654
FokI GGATG 3 cut(s) 174, 541, 571
FriOI GRGCYC 1 cut(s) 156
Fsp4HI GCNGC 1 cut(s) 654
FspBI CTAG 5 cut(s) 246, 344, 359, 650, 709
GluI GCNGC 1 cut(s) 654
HaeIII GGCC 5 cut(s) 154, 234, 253, 444, 465
HapII CCGG 6 cut(s) 132, 235, 381, 559, 589, 639
Hin1II CATG 5 cut(s) 166, 223, 421, 543, 695
HinfI GANTC 2 cut(s) 351, 528
HpaII CCGG 6 cut(s) 132, 235, 381, 559, 589, 639
HphI GGTGA 1 cut(s) 636
Hpy188I TCNGA 4 cut(s) 71, 192, 307, 699
Hpy188III TCNNGA 6 cut(s) 6, 45, 132, 282, 393, 619
HpyAV CCTTC 2 cut(s) 288, 408
HpyCH4V TGCA 3 cut(s) 187, 417, 421
HpyF10VI GCNNNNNNNGC 2 cut(s) 683, 692
HpyF3I CTNAG 4 cut(s) 31, 458, 524, 570
Hsp92II CATG 5 cut(s) 166, 223, 421, 543, 695
Kpn2I TCCGGA 1 cut(s) 131
KpnI GGTACC 1 cut(s) 366
Kzo9I GATC 3 cut(s) 51, 300, 641
LmnI GCTCC 3 cut(s) 134, 544, 700
Lsp1109I GCAGC 1 cut(s) 665
LweI GCATC 1 cut(s) 196
MaeI CTAG 5 cut(s) 246, 344, 359, 650, 709
MaeIII GTNAC 2 cut(s) 26, 410
MalI GATC 3 cut(s) 53, 302, 643
MboI GATC 3 cut(s) 51, 300, 641
MboII GAAGA 4 cut(s) 47, 299, 496, 590
MfeI CAATTG 1 cut(s) 367
MflI RGATCY 1 cut(s) 51
MhlI GDGCHC 3 cut(s) 156, 268, 704
MluCI AATT 5 cut(s) 74, 367, 404, 469, 481
MmeI TCCRAC 1 cut(s) 330
MnlI CCTC 8 cut(s) 143, 220, 224, 243, 268, 388, 592, 670
Mph1103I ATGCAT 1 cut(s) 189
MroI TCCGGA 1 cut(s) 131
MslI CAYNNNNRTG 2 cut(s) 46, 296
MspI CCGG 6 cut(s) 132, 235, 381, 559, 589, 639
MspR9I CCNGG 5 cut(s) 325, 382, 559, 589, 639
MunI CAATTG 1 cut(s) 367
MvaI CCWGG 1 cut(s) 325
MvnI CGCG 1 cut(s) 159
MwoI GCNNNNNNNGC 2 cut(s) 683, 692
NciI CCSGG 4 cut(s) 382, 559, 589, 639
NcoI CCATGG 1 cut(s) 219
NdeII GATC 3 cut(s) 51, 300, 641
NlaIII CATG 5 cut(s) 166, 223, 421, 543, 695
NlaIV GGNNCC 6 cut(s) 53, 154, 155, 172, 364, 466
NmuCI GTSAC 1 cut(s) 410
NsiI ATGCAT 1 cut(s) 189
NspI RCATGY 2 cut(s) 421, 695
PaeI GCATGC 2 cut(s) 421, 695
PaeR7I CTCGAG 1 cut(s) 205
PfeI GAWTC 2 cut(s) 351, 528
PflMI CCANNNNNTGG 1 cut(s) 474
PfoI TCCNGGA 2 cut(s) 380, 637
PkrI GCNGC 1 cut(s) 655
PpuMI RGGWCCY 1 cut(s) 170
Psp5II RGGWCCY 1 cut(s) 170
Psp6I CCWGG 1 cut(s) 323
PspGI CCWGG 1 cut(s) 323
PspN4I GGNNCC 6 cut(s) 53, 154, 155, 172, 364, 466
PspOMI GGGCCC 1 cut(s) 152
PspPI GGNCC 4 cut(s) 152, 153, 170, 464
PspPPI RGGWCCY 1 cut(s) 170
PspXI VCTCGAGB 1 cut(s) 205
PsuI RGATCY 1 cut(s) 51
RsaI GTAC 2 cut(s) 364, 494
RsaNI GTAC 2 cut(s) 363, 493
RseI CAYNNNNRTG 2 cut(s) 46, 296
SatI GCNGC 1 cut(s) 654
Sau3AI GATC 3 cut(s) 51, 300, 641
Sau96I GGNCC 4 cut(s) 152, 153, 170, 464
ScrFI CCNGG 5 cut(s) 325, 382, 559, 589, 639
SduI GDGCHC 3 cut(s) 156, 268, 704
SfaNI GCATC 1 cut(s) 196
Sfr274I CTCGAG 1 cut(s) 205
SinI GGWCC 1 cut(s) 170
SlaI CTCGAG 1 cut(s) 205
SmiMI CAYNNNNRTG 2 cut(s) 46, 296
SmlI CTYRAG 1 cut(s) 205
SmoI CTYRAG 1 cut(s) 205
SphI GCATGC 2 cut(s) 421, 695
Sse9I AATT 5 cut(s) 74, 367, 404, 469, 481
SsiI CCGC 5 cut(s) 157, 174, 333, 608, 684
SspMI CTAG 5 cut(s) 246, 344, 359, 650, 709
StyD4I CCNGG 5 cut(s) 323, 380, 557, 587, 637
StyI CCWWGG 1 cut(s) 219
TaqI TCGA 4 cut(s) 44, 206, 349, 620
TaqII GACCGA 1 cut(s) 213
TasI AATT 5 cut(s) 74, 367, 404, 469, 481
TfiI GAWTC 2 cut(s) 351, 528
TscAI CASTG 2 cut(s) 419, 528
TseFI GTSAC 1 cut(s) 410
TseI GCWGC 1 cut(s) 653
Tsp45I GTSAC 1 cut(s) 410
TspDTI ATGAA 3 cut(s) 67, 496, 590
TspRI CASTG 2 cut(s) 419, 528
Van91I CCANNNNNTGG 1 cut(s) 474
VpaK11BI GGWCC 1 cut(s) 170
XapI RAATTY 1 cut(s) 74
XceI RCATGY 2 cut(s) 421, 695
XhoI CTCGAG 1 cut(s) 205
XspI CTAG 5 cut(s) 246, 344, 359, 650, 709
Zsp2I ATGCAT 1 cut(s) 189
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.