Rmu_ssc0000118.1_g000006

Domain in Tre-2, BUB2p, and Cdc16p. Probable Rab-GAPs.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000118.1
OG0002282
Physical Location & Seq
Forward (+)
20256 .. 23380
3125 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000118.1_g000006.1.cds

Sequence Viewer

Length: 702 bp
atggcgccaggtcctattgagtcggcattgccggaatcatcgtcgtcgtcgtcgtcgtcgtgttccggcgctaactctgttgttgggagatctgcggttgaggatcttcgtttcaaggagctgagaggtgtgcaatggcgcttaaatcttgggattttgcctctttcttcttccattgatgatcttcgccgcgtcacagcggattgtcgacgaagatatgctagaatgagaagacaccttctagttgatccgcctaaggatgggagtagttctcctgatcttgccatggacaatcctctgtcccagaacccaggtgggccccgcggcatggttagcccggcctatggcatgcatccggtagaccgacacccgagctatgttgccatggtggaggtcggccggtgtctagtcgggaggctgctctcctatgctctccaagaggcttcaagagaagcaaatatgagttcaacctgggaccggaagtcgtttcagccggagatgttgttcagcgtcttggtccagatccagaagcggcgagctgacgagaacgacgtcgttgagctcattgctggagcagcaaggggaagaaggcggagagtactcctttgccgaaagtggagattggagagagagagagatagggagaaacatgatggtatcgggactgagttcaacgagtacgccgaagcagtttttggttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RabGAP-TBC

Protein Analysis

233

Amino Acids

26.05

Weight (kDa)

9.38

Isoelectric Point (pI)

68.61

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 555
AccB1I GGYRCC 1 cut(s) 4
AccI GTMKAC 2 cut(s) 208, 360
AccII CGCG 2 cut(s) 192, 324
AciI CCGC 8 cut(s) 95, 190, 200, 251, 322, 324, 532, 592
AclWI GGATC 3 cut(s) 111, 242, 517
AcoI YGGCCR 1 cut(s) 397
AcyI GRCGYC 2 cut(s) 5, 552
AfaI GTAC 2 cut(s) 600, 680
AfiI CCNNNNNNNGG 4 cut(s) 260, 328, 344, 477
AgsI TTSAA 4 cut(s) 115, 447, 468, 673
AhdI GACNNNNNGTC 1 cut(s) 481
AjnI CCWGG 3 cut(s) 7, 310, 470
AjuI GAANNNNNNNTTGG 1 cut(s) 678
AloI GAACNNNNNNTCC 2 cut(s) 488, 520
AluBI AGCT 4 cut(s) 121, 375, 539, 562
AluI AGCT 4 cut(s) 121, 375, 539, 562
Alw21I GWGCWC 1 cut(s) 564
AlwI GGATC 3 cut(s) 111, 242, 517
Ama87I CYCGRG 1 cut(s) 370
AoxI GGCC 3 cut(s) 317, 339, 397
ApaI GGGCCC 1 cut(s) 321
ApeKI GCWGC 2 cut(s) 418, 575
AspLEI GCGC 3 cut(s) 7, 71, 141
AspS9I GGNCC 5 cut(s) 11, 317, 318, 475, 517
AsuC2I CCSGG 1 cut(s) 338
AvaI CYCGRG 1 cut(s) 370
AvaII GGWCC 3 cut(s) 11, 475, 517
AxyI CCTNAGG 1 cut(s) 255
BaeGI GKGCMC 1 cut(s) 321
BanI GGYRCC 1 cut(s) 4
BanII GRGCYC 2 cut(s) 321, 564
BbsI GAAGAC 1 cut(s) 238
Bbv12I GWGCWC 1 cut(s) 564
BbvI GCAGC 2 cut(s) 405, 587
BccI CCATC 2 cut(s) 254, 647
BciT130I CCWGG 3 cut(s) 9, 312, 472
BcnI CCSGG 1 cut(s) 338
BfaI CTAG 3 cut(s) 222, 242, 407
BfoI RGCGCY 3 cut(s) 8, 72, 142
BglII AGATCT 1 cut(s) 89
BisI GCNGC 5 cut(s) 190, 325, 419, 533, 576
BlsI GCNGC 5 cut(s) 191, 326, 420, 534, 577
BmcAI AGTACT 1 cut(s) 600
Bme1390I CCNGG 4 cut(s) 9, 312, 338, 472
Bme18I GGWCC 3 cut(s) 11, 475, 517
BmeRI GACNNNNNGTC 1 cut(s) 481
BmeT110I CYCGRG 1 cut(s) 370
BmgT120I GGNCC 5 cut(s) 11, 317, 318, 475, 517
BmiI GGNNCC 4 cut(s) 6, 319, 320, 476
BmrFI CCNGG 4 cut(s) 9, 312, 338, 472
BmsI GCATC 1 cut(s) 361
BpiI GAAGAC 1 cut(s) 238
BplI GAGNNNNNCTC 2 cut(s) 256, 288
BpmI CTGGAG 1 cut(s) 591
BpuMI CCSGG 1 cut(s) 338
BsaHI GRCGYC 2 cut(s) 5, 552
BsaJI CCNNGG 5 cut(s) 285, 310, 322, 384, 471
BsaWI WCCGGW 2 cut(s) 355, 477
BsaXI ACNNNNNCTCC 2 cut(s) 488, 518
Bsc4I CCNNNNNNNGG 4 cut(s) 260, 328, 344, 477
Bse118I RCCGGY 1 cut(s) 399
Bse21I CCTNAGG 1 cut(s) 255
Bse3DI GCAATG 3 cut(s) 26, 140, 564
BseBI CCWGG 3 cut(s) 9, 312, 472
BseDI CCNNGG 5 cut(s) 285, 310, 322, 384, 471
BseGI GGATG 2 cut(s) 265, 352
BseLI CCNNNNNNNGG 4 cut(s) 260, 328, 344, 477
BseMI GCAATG 3 cut(s) 26, 140, 564
BseMII CTCAG 2 cut(s) 113, 657
BseSI GKGCMC 1 cut(s) 321
BseX3I CGGCCG 1 cut(s) 397
BseXI GCAGC 2 cut(s) 405, 587
Bsh1236I CGCG 2 cut(s) 192, 324
Bsh1285I CGRYCG 1 cut(s) 400
BshFI GGCC 3 cut(s) 319, 341, 399
BshNI GGYRCC 1 cut(s) 4
BsiEI CGRYCG 1 cut(s) 400
BsiHKAI GWGCWC 1 cut(s) 564
BsiHKCI CYCGRG 1 cut(s) 370
BsiSI CCGG 7 cut(s) 32, 66, 338, 356, 400, 478, 494
BslFI GGGAC 3 cut(s) 286, 488, 676
BslI CCNNNNNNNGG 4 cut(s) 260, 328, 344, 477
BsmFI GGGAC 3 cut(s) 286, 488, 676
BsnI GGCC 3 cut(s) 319, 341, 399
BsoBI CYCGRG 1 cut(s) 370
Bsp120I GGGCCC 1 cut(s) 317
Bsp1286I GDGCHC 2 cut(s) 321, 564
Bsp143I GATC 6 cut(s) 89, 103, 181, 247, 277, 522
Bsp19I CCATGG 2 cut(s) 285, 384
BspACI CCGC 8 cut(s) 95, 190, 200, 251, 322, 324, 532, 592
BspANI GGCC 3 cut(s) 319, 341, 399
BspCNI CTCAG 2 cut(s) 114, 658
BspFNI CGCG 2 cut(s) 192, 324
BspLI GGNNCC 4 cut(s) 6, 319, 320, 476
BspPI GGATC 3 cut(s) 111, 242, 517
BspT107I GGYRCC 1 cut(s) 4
BsrDI GCAATG 3 cut(s) 26, 140, 564
BsrFI RCCGGY 1 cut(s) 399
BssAI RCCGGY 1 cut(s) 399
BssECI CCNNGG 5 cut(s) 285, 310, 322, 384, 471
BssMI GATC 6 cut(s) 89, 103, 181, 247, 277, 522
BssNI GRCGYC 2 cut(s) 5, 552
BssT1I CCWWGG 2 cut(s) 285, 384
Bst2UI CCWGG 3 cut(s) 9, 312, 472
BstACI GRCGYC 2 cut(s) 5, 552
BstC8I GCNNGC 2 cut(s) 350, 537
BstDEI CTNAG 3 cut(s) 122, 255, 666
BstDSI CCRYGG 3 cut(s) 285, 322, 384
BstF5I GGATG 2 cut(s) 265, 352
BstFNI CGCG 2 cut(s) 192, 324
BstH2I RGCGCY 3 cut(s) 8, 72, 142
BstHHI GCGC 3 cut(s) 7, 71, 141
BstKTI GATC 6 cut(s) 92, 106, 184, 250, 280, 525
BstMBI GATC 6 cut(s) 89, 103, 181, 247, 277, 522
BstMCI CGRYCG 1 cut(s) 400
BstMWI GCNNNNNNNGC 2 cut(s) 333, 575
BstNI CCWGG 3 cut(s) 9, 312, 472
BstNSI RCATGY 1 cut(s) 352
BstSCI CCNGG 4 cut(s) 7, 310, 336, 470
BstSLI GKGCMC 1 cut(s) 321
BstUI CGCG 2 cut(s) 192, 324
BstV1I GCAGC 2 cut(s) 405, 587
BstV2I GAAGAC 1 cut(s) 238
BstX2I RGATCY 3 cut(s) 89, 103, 522
BstYI RGATCY 3 cut(s) 89, 103, 522
BstZI CGGCCG 1 cut(s) 397
Bsu36I CCTNAGG 1 cut(s) 255
BsuRI GGCC 3 cut(s) 319, 341, 399
BtgI CCRYGG 3 cut(s) 285, 322, 384
BtsCI GGATG 2 cut(s) 265, 352
Cac8I GCNNGC 2 cut(s) 350, 537
CfoI GCGC 3 cut(s) 7, 71, 141
Cfr10I RCCGGY 1 cut(s) 399
Cfr13I GGNCC 5 cut(s) 11, 317, 318, 475, 517
Cfr42I CCGCGG 1 cut(s) 325
CseI GACGC 2 cut(s) 181, 499
Csp6I GTAC 2 cut(s) 599, 679
CviAII CATG 5 cut(s) 286, 328, 349, 385, 650
CviQI GTAC 2 cut(s) 599, 679
DdeI CTNAG 3 cut(s) 122, 255, 666
DinI GGCGCC 1 cut(s) 6
DpnI GATC 6 cut(s) 91, 105, 183, 249, 279, 524
DpnII GATC 6 cut(s) 89, 103, 181, 247, 277, 522
DriI GACNNNNNGTC 1 cut(s) 481
EaeI YGGCCR 1 cut(s) 397
EagI CGGCCG 1 cut(s) 397
Eam1105I GACNNNNNGTC 1 cut(s) 481
EciI GGCGGA 2 cut(s) 240, 607
Ecl136II GAGCTC 1 cut(s) 562
EclXI CGGCCG 1 cut(s) 397
Eco130I CCWWGG 2 cut(s) 285, 384
Eco24I GRGCYC 2 cut(s) 321, 564
Eco47I GGWCC 3 cut(s) 11, 475, 517
Eco52I CGGCCG 1 cut(s) 397
Eco53kI GAGCTC 1 cut(s) 562
Eco81I CCTNAGG 1 cut(s) 255
Eco88I CYCGRG 1 cut(s) 370
EcoICRI GAGCTC 1 cut(s) 562
EcoO109I RGGNCCY 2 cut(s) 11, 318
EcoRII CCWGG 3 cut(s) 7, 310, 470
EcoT14I CCWWGG 2 cut(s) 285, 384
EcoT22I ATGCAT 1 cut(s) 354
EcoT38I GRGCYC 2 cut(s) 321, 564
EgeI GGCGCC 1 cut(s) 6
EheI GGCGCC 1 cut(s) 6
ErhI CCWWGG 2 cut(s) 285, 384
FaeI CATG 5 cut(s) 289, 331, 352, 388, 653
FaqI GGGAC 3 cut(s) 286, 488, 676
FatI CATG 5 cut(s) 285, 327, 348, 384, 649
FauI CCCGC 1 cut(s) 329
FblI GTMKAC 2 cut(s) 208, 360
Fnu4HI GCNGC 5 cut(s) 190, 325, 419, 533, 576
FokI GGATG 2 cut(s) 272, 339
FriOI GRGCYC 2 cut(s) 321, 564
Fsp4HI GCNGC 5 cut(s) 190, 325, 419, 533, 576
FspBI CTAG 3 cut(s) 222, 242, 407
GlaI GCGC 3 cut(s) 6, 70, 140
GluI GCNGC 5 cut(s) 190, 325, 419, 533, 576
GsuI CTGGAG 1 cut(s) 591
HaeII RGCGCY 3 cut(s) 8, 72, 142
HaeIII GGCC 3 cut(s) 319, 341, 399
HapII CCGG 7 cut(s) 32, 66, 338, 356, 400, 478, 494
HgaI GACGC 2 cut(s) 181, 499
HhaI GCGC 3 cut(s) 7, 71, 141
Hin1I GRCGYC 2 cut(s) 5, 552
Hin1II CATG 5 cut(s) 289, 331, 352, 388, 653
Hin6I GCGC 3 cut(s) 5, 69, 139
HinP1I GCGC 3 cut(s) 5, 69, 139
HincII GTYRAC 1 cut(s) 209
HindII GTYRAC 1 cut(s) 209
HinfI GANTC 2 cut(s) 20, 35
HpaII CCGG 7 cut(s) 32, 66, 338, 356, 400, 478, 494
Hpy166II GTNNAC 2 cut(s) 209, 361
Hpy188III TCNNGA 6 cut(s) 275, 412, 447, 520, 526, 661
Hpy8I GTNNAC 2 cut(s) 209, 361
Hpy99I CGWCG 9 cut(s) 46, 49, 52, 55, 58, 61, 213, 554, 557
HpyAV CCTTC 2 cut(s) 248, 582
HpyCH4IV ACGT 1 cut(s) 552
HpyCH4V TGCA 2 cut(s) 133, 352
HpyF10VI GCNNNNNNNGC 2 cut(s) 333, 575
HpyF3I CTNAG 3 cut(s) 122, 255, 666
HpySE526I ACGT 1 cut(s) 552
Hsp92I GRCGYC 2 cut(s) 5, 552
Hsp92II CATG 5 cut(s) 289, 331, 352, 388, 653
HspAI GCGC 3 cut(s) 5, 69, 139
KasI GGCGCC 1 cut(s) 4
KspI CCGCGG 1 cut(s) 325
Kzo9I GATC 6 cut(s) 89, 103, 181, 247, 277, 522
LmnI GCTCC 2 cut(s) 118, 572
Lsp1109I GCAGC 2 cut(s) 405, 587
LweI GCATC 1 cut(s) 361
MaeI CTAG 3 cut(s) 222, 242, 407
MaeII ACGT 1 cut(s) 552
MaeIII GTNAC 1 cut(s) 193
MalI GATC 6 cut(s) 91, 105, 183, 249, 279, 524
MboI GATC 6 cut(s) 89, 103, 181, 247, 277, 522
MboII GAAGA 7 cut(s) 98, 159, 162, 176, 225, 243, 597
MflI RGATCY 3 cut(s) 89, 103, 522
MhlI GDGCHC 2 cut(s) 321, 564
Mly113I GGCGCC 1 cut(s) 5
MlyI GAGTC 1 cut(s) 29
MnlI CCTC 7 cut(s) 94, 119, 171, 306, 385, 408, 433
Mph1103I ATGCAT 1 cut(s) 354
MseI TTAA 1 cut(s) 143
MspA1I CMGCKG 2 cut(s) 200, 324
MspI CCGG 7 cut(s) 32, 66, 338, 356, 400, 478, 494
MspR9I CCNGG 4 cut(s) 9, 312, 338, 472
MvaI CCWGG 3 cut(s) 9, 312, 472
MvnI CGCG 2 cut(s) 192, 324
MwoI GCNNNNNNNGC 2 cut(s) 333, 575
NarI GGCGCC 1 cut(s) 5
NciI CCSGG 1 cut(s) 338
NcoI CCATGG 2 cut(s) 285, 384
NdeII GATC 6 cut(s) 89, 103, 181, 247, 277, 522
NlaIII CATG 5 cut(s) 289, 331, 352, 388, 653
NlaIV GGNNCC 4 cut(s) 6, 319, 320, 476
NmuCI GTSAC 1 cut(s) 193
NsiI ATGCAT 1 cut(s) 354
NspI RCATGY 1 cut(s) 352
PaeI GCATGC 1 cut(s) 352
PcsI WCGNNNNNNNCGW 6 cut(s) 47, 50, 53, 56, 549, 681
PfeI GAWTC 1 cut(s) 35
PkrI GCNGC 5 cut(s) 191, 326, 420, 534, 577
PleI GAGTC 1 cut(s) 28
PluTI GGCGCC 1 cut(s) 8
PpsI GAGTC 1 cut(s) 28
PpuMI RGGWCCY 1 cut(s) 11
Psp124BI GAGCTC 1 cut(s) 564
Psp5II RGGWCCY 1 cut(s) 11
Psp6I CCWGG 3 cut(s) 7, 310, 470
PspGI CCWGG 3 cut(s) 7, 310, 470
PspN4I GGNNCC 4 cut(s) 6, 319, 320, 476
PspOMI GGGCCC 1 cut(s) 317
PspPI GGNCC 5 cut(s) 11, 317, 318, 475, 517
PspPPI RGGWCCY 1 cut(s) 11
PsuI RGATCY 3 cut(s) 89, 103, 522
RsaI GTAC 2 cut(s) 600, 680
RsaNI GTAC 2 cut(s) 599, 679
SacI GAGCTC 1 cut(s) 564
SacII CCGCGG 1 cut(s) 325
SalI GTCGAC 1 cut(s) 207
SaqAI TTAA 1 cut(s) 143
SatI GCNGC 5 cut(s) 190, 325, 419, 533, 576
Sau3AI GATC 6 cut(s) 89, 103, 181, 247, 277, 522
Sau96I GGNCC 5 cut(s) 11, 317, 318, 475, 517
ScaI AGTACT 1 cut(s) 600
SchI GAGTC 1 cut(s) 29
ScrFI CCNGG 4 cut(s) 9, 312, 338, 472
SduI GDGCHC 2 cut(s) 321, 564
SfaNI GCATC 1 cut(s) 361
SfoI GGCGCC 1 cut(s) 6
Sfr303I CCGCGG 1 cut(s) 325
SgrBI CCGCGG 1 cut(s) 325
SinI GGWCC 3 cut(s) 11, 475, 517
SphI GCATGC 1 cut(s) 352
SsiI CCGC 8 cut(s) 95, 190, 200, 251, 322, 324, 532, 592
SspDI GGCGCC 1 cut(s) 4
SspMI CTAG 3 cut(s) 222, 242, 407
SstI GAGCTC 1 cut(s) 564
StyD4I CCNGG 4 cut(s) 7, 310, 336, 470
StyI CCWWGG 2 cut(s) 285, 384
TaiI ACGT 1 cut(s) 555
TaqI TCGA 1 cut(s) 208
TaqII GACCGA 1 cut(s) 378
TatI WGTACW 1 cut(s) 598
TauI GCSGC 3 cut(s) 192, 327, 535
TfiI GAWTC 1 cut(s) 35
Tru1I TTAA 1 cut(s) 143
Tru9I TTAA 1 cut(s) 143
TseFI GTSAC 1 cut(s) 193
TseI GCWGC 2 cut(s) 418, 575
Tsp45I GTSAC 1 cut(s) 193
VpaK11BI GGWCC 3 cut(s) 11, 475, 517
XceI RCATGY 1 cut(s) 352
XcmI CCANNNNNNNNNTGG 1 cut(s) 311
XmiI GTMKAC 2 cut(s) 208, 360
XspI CTAG 3 cut(s) 222, 242, 407
ZraI GACGTC 1 cut(s) 553
ZrmI AGTACT 1 cut(s) 600
Zsp2I ATGCAT 1 cut(s) 354
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.